CN106010966A - Multidirectional tissue-engineering porous material perfusion system - Google Patents
Multidirectional tissue-engineering porous material perfusion system Download PDFInfo
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- CN106010966A CN106010966A CN201610404357.5A CN201610404357A CN106010966A CN 106010966 A CN106010966 A CN 106010966A CN 201610404357 A CN201610404357 A CN 201610404357A CN 106010966 A CN106010966 A CN 106010966A
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- 239000011148 porous material Substances 0.000 title claims abstract description 71
- 230000010412 perfusion Effects 0.000 title claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 230000002572 peristaltic effect Effects 0.000 claims description 24
- 235000016709 nutrition Nutrition 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 abstract description 12
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 19
- 230000000694 effects Effects 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 230000017423 tissue regeneration Effects 0.000 description 3
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 239000011664 nicotinic acid Substances 0.000 description 2
- -1 ECM) Proteins 0.000 description 1
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 102000007072 Nerve Growth Factors Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000003900 neurotrophic factor Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000033667 organ regeneration Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Dermatology (AREA)
- Immunology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- External Artificial Organs (AREA)
- Media Introduction/Drainage Providing Device (AREA)
Abstract
The invention discloses a multidirectional tissue-engineering porous material perfusion system, comprising a nutrient solution container, a first conduit, a first perfusion chamber joint, a perfusion chamber, an outer sleeve pipe, a first negative-pressure unit, a negative-pressure conduit and a tissue-engineering porous material. According to the invention, the tissue-engineering porous material is arranged in the perfusion chamber, and a nutrient solution flows in the perfusion chamber in virtue of the negative-pressure unit, so pressure contact between the tissue-engineering porous material and the nutrient solution is realized; and thus, the speed and uniformity of nutrient solution absorption by the tissue-engineering porous material is improved. A multistage pump is employed, so the capacity of the tissue-engineering porous material in bearing liquid in the perfusion chamber can be adjusted; and thus, the nutrient solution absorption speed of the tissue-engineering porous material is controlled.
Description
Technical field
The present invention relates to tissue engineering technique field, a kind of multi-direction organizational project porous material perfusion system.
Background technology
It is current for using organizational project principle build bio-imitability organizational project reparation/graft materials to carry out tissue/organ Regeneration and Repair
Study hotspot.Its ultimate principle is: first passes through the complex art preparations such as physics, chemistry, biology and has bionic structure
Tissue engineering bracket/repair materials, usually porous material；Secondly it is dipped to promote tissue regeneration by porous material
In nutrient solution, such as some extracellular matrix (extracellular matrix, ECM), neurotrophic factor, medicine or dry
The solution of cell or mixed solution, make organizational project porous material obtain biological activity bionic；Finally by obtained bio-imitability group
Knit engineering material to be implanted to defect/injured tissue site and carry out tissue repair, or obtained bio-imitability tissue engineering material is planted
After target cell, carry out in vitro being implanted to defect/injured tissue site again after cell cultivates certain time.
In prior art, the method that organizational project porous material carries out nutrient substance load or cell seeding mainly will tissue
Engineering stephanoporate material is immersed in nutrient solution and carries out nature absorption carriage.Use this kind of mode, organizational project porous material
Material and nutrient solution, mainly complete load by passive adsorption effect, it is contemplated that various organizational project porous materials
Pore size differs, differing property of shape, this mode of loading, is typically difficult to make nutrient substance fully, uniformly, effectively bear
It is loaded in organizational project porous material, and loading speed is slow, does not reaches nutrient substance load effect.Especially for containing longitudinally
, its load efficiency, effect are worse for the organizational project porous of hole path and horizontal hole.
It is accordingly required in particular to a kind of nutrient substance or Cell infusion organizational project porous material perfusion system, to solve existing skill
Problem present in art.
Summary of the invention
The invention aims to solve in prior art, slow and uneven the lacking of speed cultivated by organizational project porous material
Fall into, it is provided that a kind of multi-direction organizational project porous material perfusion system, solve problems of the prior art.
For achieving the above object, technical scheme is as follows:
A kind of multi-direction organizational project porous material perfusion system, connects including reservoir, the first conduit, the first filling cavity
Head, filling cavity, trocar sheath, the first negative pressure equipment, negative pressure catheter and organizational project porous material, described reservoir is even
One end of logical first conduit, the other end of described first conduit one end by the first filling cavity fittings filling cavity, described
The sheathed trocar sheath in outside of filling cavity, forms a negative pressure cavity between medial part and the lateral part of filling cavity of described trocar sheath,
Multiple hole making negative pressure cavity and filling cavity inside connect, one end connection of described negative pressure catheter are set on the sidepiece of described filling cavity
Negative pressure cavity, the other end arranges the first negative pressure equipment, described first negative pressure equipment make reservoir in nutritional solution successively by first
Conduit, filling cavity, hole and negative pressure cavity flow in negative pressure catheter, arrange organizational project porous material in described filling cavity.
In the present invention, also include the second filling cavity joint, the second conduit and the second negative pressure equipment, another of described filling cavity
Hold by one end of second filling cavity fittings the second conduit, described second negative pressure equipment make in reservoir nutritional solution by
First conduit is flowed in the second conduit by filling cavity.
In the present invention, also include that the 3rd conduit, described first negative pressure equipment are the first peristaltic pump, described first peristaltic pump bag
Containing the first inlet and the first liquid outlet, the other end of the first inlet connection negative pressure catheter of described first peristaltic pump, first
One end of liquid outlet connection the 3rd conduit, the other end connection reservoir of described 3rd conduit.
In the present invention, described first peristaltic pump is multistage peristaltic pump.
In the present invention, also include that the 4th conduit, described second negative pressure equipment are the second peristaltic pump, described second peristaltic pump bag
Containing the second inlet and the second liquid outlet, the second inlet of described second peristaltic pump connects the other end of the second conduit, and second
One end of liquid outlet connection the 3rd conduit, the other end connection reservoir of described 3rd conduit.
In the present invention, also include that returnable bottle, the described other end of the second conduit and/or the other end of negative pressure catheter are arranged back
Receiving bottle, described negative pressure catheter and the second conduit are respectively communicated with returnable bottle.
In the present invention, in described filling cavity, organizational project porous material is arranged on the first filling cavity joint and the second perfusion
Between the joint of chamber.
In the present invention, described organizational project porous material comprise be positioned at the first end of filling cavity one end and be positioned at filling cavity another
The second end of one end, the first end of described organizational project porous material and the second end be close to respectively filling cavity inwall,
On outer wall, filling cavity one end and/or the filling cavity other end, described first filling cavity joint and the second filling cavity joint lead to respectively
First end and the second end of crossing organizational project porous material connect filling cavity.
In the present invention, described first filling cavity joint and the second filling cavity joint are respectively by the of organizational project porous material
One end and the second end clamping and/or threaded filling cavity.
In the present invention, described first filling cavity joint and the second filling cavity joint comprise tapered pole, described first perfusion respectively
Chamber joint and the second filling cavity joint connect filling cavity by tapered pole respectively.
Beneficial effect
1, organizational project porous material is arranged in filling cavity by the present invention, and nutritional solution is flowed at filling cavity by negative pressure equipment
In, it is achieved the pressure contact between organizational project porous material and nutritional solution, and then it is greatly improved the suction of organizational project porous material
Receive speed and the uniformity of nutritional solution；
2, multiple holes on the sidepiece of filling cavity, make nutritional solution be permeated by hole, reach organizational project porous material and longitudinally soak
Effect；
3, multistage pump is used, it is possible to adjust organizational project porous material in filling cavity, bear the size of liquid, and then in group
Weaver's journey porous material controls its speed absorbing nutritional solution.
Accompanying drawing explanation
The structural representation of Fig. 1 present invention；
Fig. 2 is the structural representation in the A portion of Fig. 1；
The structural representation of the organizational project porous material arranged in Fig. 3 filling cavity of the present invention；
Fig. 4 is the axonometric chart of Fig. 1；
Fig. 5 present invention uses schematic diagram during peristaltic pump structure；
Fig. 6 present invention uses schematic diagram during returnable bottle structure.
In figure: 1, reservoir；2, the first conduit；3, the first filling cavity joint；4, filling cavity；5、
Trocar sheath；6, the first negative pressure equipment；7, negative pressure catheter；8, organizational project porous material；9, negative pressure cavity；
10, hole；11, the second conduit；12, the second negative pressure equipment；13, the 3rd conduit；14, the first inlet；
15, the first liquid outlet；16, the 4th conduit；17, the second inlet；18, the second liquid outlet；19, reclaim
Bottle；20, the second filling cavity joint.
Detailed description of the invention
In the view of whole accompanying drawings, corresponding reference marks represents corresponding parts.
A kind of multi-direction organizational project porous material perfusion system, irrigates including reservoir the 1, first conduit 2, first
Chamber joint 3, filling cavity 4, trocar sheath the 5, first negative pressure equipment 6, negative pressure catheter 7 and organizational project porous material 8.
Reservoir 1 connects one end of the first conduit 2, and the other end of the first conduit 2 is by the first filling cavity joint 3 even
One end of logical filling cavity 4, the sheathed trocar sheath in outside 5 of filling cavity 4, the medial part of trocar sheath 5 and the outside of filling cavity 4
Form a negative pressure cavity 9 between portion, the sidepiece of filling cavity 4 is arranged and multiple makes to connect inside negative pressure cavity 9 and filling cavity 4
Hole 10, one end connection negative pressure cavity 9 of negative pressure catheter 7, the other end arranges the first negative pressure equipment 6, and the first negative pressure equipment 6 makes
In reservoir 1, nutritional solution is flow in negative pressure catheter 7 by the first conduit 2, filling cavity 4, hole 10 and negative pressure cavity 9 successively,
Organizational project porous material 8 is set in filling cavity 4.
Organizational project porous material 8 is arranged in filling cavity 4 by the present invention, and nutritional solution is flowed at filling cavity by negative pressure equipment
In 4, it is achieved the pressure contact between organizational project porous material 8 and nutritional solution, and then it is greatly improved organizational project porous material
The speed of material 8 absorption nutritional solution and the uniformity；
Multiple holes 10 on the sidepiece of filling cavity 4, make nutritional solution pass through hole 10 and permeate, and reach organizational project porous material 8 and indulge
To the effect soaked.
Embodiment 1
Multi-direction organizational project porous material perfusion system, also includes second filling cavity joint the 20, second conduit 11 and second
Negative pressure equipment 12, the other end of filling cavity 4 connects one end of the second conduit 11 by the second filling cavity joint 20, and second is negative
In pressure equipment 12 makes reservoir 1, nutritional solution is flowed in the second conduit 11 by filling cavity 4 by the first conduit 2, reaches
The effect that organizational project porous material 8 laterally soaks.
Embodiment 2
Multi-direction organizational project porous material perfusion system, also includes that the 3rd conduit 13, the first negative pressure equipment 6 are first compacted
Dynamic pump, the first peristaltic pump comprises the first inlet 14 and the first liquid outlet 15, and the first inlet 14 of the first peristaltic pump is even
The other end of logical negative pressure catheter 7, the first liquid outlet 15 connects one end of the 3rd conduit 13, the other end of the 3rd conduit 13
Connection reservoir 1.
First peristaltic pump is multistage peristaltic pump；Use multistage pump and multistage peristaltic pump structure structure, it is possible to adjust organizational project many
Porous materials bears the size of fluid pressure in filling cavity, and then controls its absorption nutritional solution in organizational project porous material
Speed.
Embodiment 3
Multi-direction organizational project porous material perfusion system, also includes that the 4th conduit 16, the second negative pressure equipment 12 are second compacted
Dynamic pump, the second peristaltic pump comprises the second inlet 17 and the second liquid outlet 18, and the second inlet 17 of the second peristaltic pump is even
The other end of logical second conduit 11, the second liquid outlet 18 connects one end of the 3rd conduit 13, the other end of the 3rd conduit 13
Connection reservoir 1, reaches the effect that nutritional solution recycles.
Embodiment 4
Multi-direction organizational project porous material perfusion system, also includes returnable bottle 19, the other end of the second conduit 11 and/or negative
The other end of pressure conduit 7 arranges returnable bottle 19, negative pressure catheter 7 and the second conduit 11 and connects returnable bottle by conduit 21 respectively
19。
In filling cavity 4, organizational project porous material 8 is arranged on the first filling cavity joint 3 and the second filling cavity joint 20
Between.
In preferred embodiment:
Organizational project porous material 8 comprises the first end being positioned at filling cavity 4 one end and is positioned at the second of filling cavity 4 other end
End, the first end of organizational project porous material 8 and the second end are close to filling cavity 4 inwall, outer wall, perfusion respectively
On one end, chamber 4 and/or filling cavity 4 other end, the first filling cavity joint 3 and the second filling cavity joint 20 are respectively by tissue
The first end of engineering stephanoporate material 8 and the second end connect filling cavity 4.
In preferred embodiment, the first filling cavity joint 3 and the second filling cavity joint 20 are respectively by organizational project porous
The first end of material 8 and the second end clamping and/or threaded filling cavity 4.
First filling cavity joint 3 and the second filling cavity joint 20 comprise tapered pole, the first filling cavity joint 3 and second respectively
Filling cavity joint 20 connects filling cavity 4 by tapered pole respectively, reaches the effect conveniently connecting and being fastenedly connected.
The present invention, the first negative pressure equipment 6 and the second negative pressure equipment can use identical negative pressure structure or different negative pressure structure,
All can reach the perfusion on different directions.
The above-mentioned description to embodiment is to be understood that for ease of those skilled in the art and use the present invention.Ripe
These embodiments obviously easily can be made various amendment by the personnel knowing art technology, and described herein the most former
Ought to use in other embodiments without through performing creative labour.Therefore, the invention is not restricted to above-described embodiment, ability
Field technique personnel should be the present invention's according to the announcement of the present invention, the improvement made without departing from scope and amendment
Within protection domain.
Claims (10)
1. multi-direction organizational project porous material perfusion system, it is characterised in that: include reservoir, first lead
Pipe, the first filling cavity joint, filling cavity, trocar sheath, the first negative pressure equipment, negative pressure catheter and organizational project porous material,
Described reservoir connects one end of the first conduit, and the other end of described first conduit is filled by the first filling cavity fittings
The one end in note chamber, the sheathed trocar sheath in outside of described filling cavity, between medial part and the lateral part of filling cavity of described trocar sheath
Form a negative pressure cavity, the sidepiece of described filling cavity arranges multiple internal hole connected of negative pressure cavity and filling cavity that makes, described negative
One end connection negative pressure cavity of pressure conduit, the other end arranges the first negative pressure equipment, in described first negative pressure equipment makes reservoir
Nutritional solution is flow in negative pressure catheter by the first conduit, filling cavity, hole and negative pressure cavity successively, arranges tissue work in described filling cavity
Journey porous material.
Multi-direction organizational project porous material the most according to claim 1 perfusion system, it is characterised in that: also include
Second filling cavity joint, the second conduit and the second negative pressure equipment, the other end of described filling cavity is by the second filling cavity joint even
One end of logical second conduit, in described second negative pressure equipment makes reservoir, nutritional solution is flowed into by filling cavity by the first conduit
In second conduit.
Multi-direction organizational project porous material the most according to claim 1 perfusion system, it is characterised in that: also include
3rd conduit, described first negative pressure equipment is the first peristaltic pump, and described first peristaltic pump comprises the first inlet and first and goes out liquid
Mouthful, the other end of the first inlet connection negative pressure catheter of described first peristaltic pump, the one of the first liquid outlet connection the 3rd conduit
End, the other end connection reservoir of described 3rd conduit.
Multi-direction organizational project porous material the most according to claim 3 perfusion system, it is characterised in that: described the
One peristaltic pump is multistage peristaltic pump.
Multi-direction organizational project porous material the most according to claim 2 perfusion system, it is characterised in that: also include
4th conduit, described second negative pressure equipment is the second peristaltic pump, and described second peristaltic pump comprises the second inlet and second and goes out liquid
Mouthful, the second inlet of described second peristaltic pump connects the other end of the second conduit, the one of the second liquid outlet connection the 3rd conduit
End, the other end connection reservoir of described 3rd conduit.
Multi-direction organizational project porous material the most according to claim 2 perfusion system, it is characterised in that: also include
Returnable bottle, the described other end of the second conduit and/or the other end of negative pressure catheter arrange returnable bottle, described negative pressure catheter and
Two conduits are respectively communicated with returnable bottle.
7. according to the arbitrary described multi-direction organizational project porous material perfusion system of claim 1 to 6, it is characterised in that:
In described filling cavity, organizational project porous material is arranged between the first filling cavity joint and the second filling cavity joint.
8. according to the arbitrary described multi-direction organizational project porous material perfusion system of claim 1 to 6, it is characterised in that:
Described organizational project porous material comprises the first end being positioned at filling cavity one end and the second end being positioned at the filling cavity other end,
The first end of described organizational project porous material and the second end are close to filling cavity inwall, outer wall, filling cavity one end respectively
And/or on the filling cavity other end, described first filling cavity joint and the second filling cavity joint are respectively by organizational project porous material
The first end of material and the second end connect filling cavity.
Multi-direction organizational project porous material the most according to claim 8 perfusion system, it is characterised in that: described the
One filling cavity joint and the second filling cavity joint are respectively by first end and the second end clamping of organizational project porous material
And/or threaded filling cavity.
Multi-direction organizational project porous material the most according to claim 8 perfusion system, it is characterised in that: described the
One filling cavity joint and the second filling cavity joint comprise tapered pole, described first filling cavity joint and the second filling cavity joint respectively
Filling cavity is connected respectively by tapered pole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610404357.5A CN106010966B (en) | 2016-06-08 | 2016-06-08 | System is perfused in multi-direction organizational project porous material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610404357.5A CN106010966B (en) | 2016-06-08 | 2016-06-08 | System is perfused in multi-direction organizational project porous material |
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| Publication Number | Publication Date |
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| CN106010966A true CN106010966A (en) | 2016-10-12 |
| CN106010966B CN106010966B (en) | 2019-03-15 |
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| CN201610404357.5A Expired - Fee Related CN106010966B (en) | 2016-06-08 | 2016-06-08 | System is perfused in multi-direction organizational project porous material |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085254A (en) * | 2018-02-09 | 2018-05-29 | 中国人民解放军第四军医大学 | Tissue engineering product culture apparatus |
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2016
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085254A (en) * | 2018-02-09 | 2018-05-29 | 中国人民解放军第四军医大学 | Tissue engineering product culture apparatus |
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| CN106010966B (en) | 2019-03-15 |
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