CN205924550U - Novel multi-direction tissue engineering porous material perfusion system - Google Patents
Novel multi-direction tissue engineering porous material perfusion system Download PDFInfo
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- CN205924550U CN205924550U CN201620555886.0U CN201620555886U CN205924550U CN 205924550 U CN205924550 U CN 205924550U CN 201620555886 U CN201620555886 U CN 201620555886U CN 205924550 U CN205924550 U CN 205924550U
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- Prior art keywords
- filling cavity
- porous material
- negative pressure
- conduit
- organizational project
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- 239000011148 porous material Substances 0.000 title claims abstract description 71
- 230000010412 perfusion Effects 0.000 title claims abstract description 19
- 235000015097 nutrients Nutrition 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 230000002572 peristaltic effect Effects 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 8
- 239000011797 cavity material Substances 0.000 claims 37
- 235000016709 nutrition Nutrition 0.000 claims 1
- 230000035764 nutrition Effects 0.000 claims 1
- 239000000243 solution Substances 0.000 description 21
- 230000000694 effects Effects 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- -1 ECM) Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 102000007072 Nerve Growth Factors Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000003900 neurotrophic factor Substances 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000033667 organ regeneration Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
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Abstract
The utility model discloses a novel multi-direction tissue engineering porous material system of infusing, including nutrient solution container, first pipe, first filling the chamber joint, fill chamber, outer tube, first negative pressure equipment, negative pressure pipe and tissue engineering porous material. The utility model arranges the porous material of tissue engineering in the perfusion cavity, and the nutrient solution flows in the perfusion cavity through the negative pressure equipment, thereby realizing the pressure contact between the porous material of tissue engineering and the nutrient solution; thereby greatly improving the speed and the uniformity of the porous material of the tissue engineering for absorbing the nutrient solution; the multistage pump is adopted, so that the liquid bearing size of the tissue engineering porous material in the perfusion cavity can be adjusted, and the speed of the tissue engineering porous material for absorbing nutrient solution is controlled.
Description
Technical field
The utility model is related to tissue engineering technique field, specifically a kind of new multi-direction organizational project porous material
Material perfusion system.
Background technology
Build bio-imitability organizational project reparation/graft materials with organizational project principle and carry out tissue/organ Regeneration and Repair
It is current study hotspot.Its general principle is：First pass through the complex arts such as physics, chemistry, biology preparation have structure imitate
Tissue engineering bracket/the repair materials of natural disposition, usually porous material；Secondly being dipped to porous material can promotion organization regeneration
Nutrient solution in, such as some extracellular matrixs (extracellular matrix, ECM), neurotrophic factor, medicine
Or the solution of stem cell or mixed solution, make organizational project porous material obtain biological activity bionic；Finally will be obtained bionical
Property tissue engineering material is implanted to defect/injured tissue site and carries out tissue repair, or by obtained bio-imitability tissue engineering material
After plantation target cell, after carrying out cell culture certain time in vitro, it is implanted to defect/injured tissue site again.
In prior art, organizational project porous material is carried out with nutriment load or the method for cell seeding mainly will
Organizational project porous material is immersed in nutrient solution and carries out nature absorption carriage.Using this kind of mode, organizational project is many
Porous materials and nutrient solution, mainly act on by passive adsorption completing load it is contemplated that various organizational project porous material
The pore size of material differs, differing property of shape, this mode of loading, is typically difficult to make nutriment fully, uniformly, effectively bear
It is loaded in organizational project porous material, and loading speed is slow, does not reach nutriment load effect.Especially for containing longitudinal hole
, its load efficiency, effect are worse for the organizational project porous of passage and horizontal hole.
It is accordingly required in particular to a kind of nutriment or Cell infusion organizational project porous material perfusion system, existing to solve
There is problem present in technology.
Utility model content
The purpose of this utility model is to solve in prior art, and organizational project porous material culture speed is slow and uneven
Even defect, provides a kind of new multi-direction organizational project porous material to irrigate system, to solve to ask present in prior art
Topic.
For achieving the above object, the technical solution of the utility model is as follows：
A kind of new multi-direction organizational project porous material irrigates system, including reservoir, the first conduit, the first filling
Note chamber joint, filling cavity, outer tube, the first negative pressure equipment, negative pressure catheter and organizational project porous material, described reservoir
Connect one end of the first conduit, the other end of described first conduit passes through one end of the first filling cavity fittings filling cavity, institute
State the sheathed outer tube in outside of filling cavity, between the inside portion of described outer tube and the outside portion of filling cavity, form a negative pressure
Chamber, the sidepiece of described filling cavity arranges multiple holes making negative pressure cavity and connecting inside filling cavity, one end of described negative pressure catheter
Connection negative pressure cavity, the other end arranges the first negative pressure equipment, described first negative pressure equipment make in reservoir nutrient solution successively by
First conduit, filling cavity, hole and negative pressure cavity flow in negative pressure catheter, setting organizational project porous material in described filling cavity.
In the utility model, also include the second filling cavity joint, the second conduit and the second negative pressure equipment, described filling cavity
The other end by one end of second filling cavity fittings the second conduit, described second negative pressure equipment makes battalion in reservoir
Nutrient solution is flowed in the second conduit by filling cavity by the first conduit.
In the utility model, also include the 3rd conduit, described first negative pressure equipment is the first peristaltic pump, and described first is compacted
Dynamic pump comprises the first inlet and the first liquid outlet, and the first inlet of described first peristaltic pump connects the another of negative pressure catheter
End, the first liquid outlet connects one end of the 3rd conduit, the other end connection reservoir of described 3rd conduit.
In the utility model, described first peristaltic pump is multistage peristaltic pump.
In the utility model, also include the 4th conduit, described second negative pressure equipment is the second peristaltic pump, and described second is compacted
Dynamic pump comprises the second inlet and the second liquid outlet, and the second inlet of described second peristaltic pump connects the another of the second conduit
End, the second liquid outlet connects one end of the 3rd conduit, the other end connection reservoir of described 3rd conduit.
In the utility model, also include returnable bottle, the other end of described second conduit and/or the other end of negative pressure catheter
Setting returnable bottle, described negative pressure catheter and the second conduit are respectively communicated with returnable bottle.
In the utility model, in described filling cavity, organizational project porous material be arranged on the first filling cavity joint and
Between second filling cavity joint.
In the utility model, described organizational project porous material comprises the first end positioned at filling cavity one end and is located at
The second end of the filling cavity other end, the first end of described organizational project porous material and the second end are close to perfusion respectively
On cavity wall, outer wall, filling cavity one end and/or the filling cavity other end, described first filling cavity joint and the second filling cavity joint
Pass through the first end of organizational project porous material respectively and the second end connects filling cavity.
In the utility model, described first filling cavity joint and the second filling cavity joint pass through organizational project porous respectively
The first end of material and the second end clamping and/or threaded filling cavity.
In the utility model, described first filling cavity joint and the second filling cavity joint comprise tapered pole respectively, described
First filling cavity joint and the second filling cavity joint connect filling cavity by tapered pole respectively.
Beneficial effect
1st, organizational project porous material is arranged in filling cavity the utility model, and nutrient solution is flowed by negative pressure equipment
In filling cavity, realize the pressure contact between organizational project porous material and nutrient solution, and then greatly improve organizational project porous
Material absorbs speed and the uniformity of nutrient solution；
2nd, multiple holes on the sidepiece of filling cavity, make nutrient solution permeate by hole, reach organizational project porous material and longitudinally soak
The effect of bubble；
3rd, adopt multistage pump, the size that organizational project porous material bears liquid in filling cavity, Jin Er can be adjusted
It is controlled to absorb the speed of nutrient solution in organizational project porous material.
Brief description
Fig. 1 structural representation of the present utility model；
Fig. 2 is the structural representation in the A portion of Fig. 1；
The structural representation of the organizational project porous material of setting in Fig. 3 the utility model filling cavity；
Fig. 4 is the stereogram of Fig. 1；
Fig. 5 the utility model adopts schematic diagram during peristaltic pump structure；
Fig. 6 the utility model is using the schematic diagram reclaiming during bottle structure.
In figure：1st, reservoir；2nd, the first conduit；3rd, the first filling cavity joint；4th, filling cavity；5th, outer tube；6th,
One negative pressure equipment；7th, negative pressure catheter；8th, organizational project porous material；9th, negative pressure cavity；10th, hole；11st, the second conduit；12nd, second is negative
Pressure equipment；13rd, the 3rd conduit；14th, the first inlet；15th, the first liquid outlet；16th, the 4th conduit；17th, the second inlet；18、
Second liquid outlet；19th, returnable bottle；20th, the second filling cavity joint.
Specific embodiment
In the view of whole accompanying drawings, corresponding reference symbol represents corresponding part.
A kind of new multi-direction organizational project porous material irrigates system, including reservoir 1, the first conduit 2, first
Filling cavity joint 3, filling cavity 4, outer tube 5, the first negative pressure equipment 6, negative pressure catheter 7 and organizational project porous material 8.
Reservoir 1 connects one end of the first conduit 2, and the other end of the first conduit 2 passes through the first filling cavity joint 3 even
One end of logical filling cavity 4, the sheathed outer tube in outside 5 of filling cavity 4, between the outside portion of the inside portion of outer tube 5 and filling cavity 4
Form a negative pressure cavity 9, the sidepiece of filling cavity 4 is arranged multiple holes 10 making negative pressure cavity 9 be connected with filling cavity 4 inside, negative pressure
One end connection negative pressure cavity 9 of conduit 7, the other end arranges the first negative pressure equipment 6, and the first negative pressure equipment 6 makes battalion in reservoir 1
Nutrient solution is flow in negative pressure catheter 7 by the first conduit 2, filling cavity 4, hole 10 and negative pressure cavity 9 successively, setting group weaver in filling cavity 4
Journey porous material 8.
Organizational project porous material 8 is arranged in filling cavity 4 the utility model, and nutrient solution is flowed by negative pressure equipment
In filling cavity 4, realize the pressure contact between organizational project porous material 8 and nutrient solution, and then it is many to greatly improve organizational project
Porous materials 8 absorb speed and the uniformity of nutrient solution；
Multiple holes 10 on the sidepiece of filling cavity 4, make nutrient solution pass through hole 10 and permeate, and reach organizational project porous material 8 and indulge
To the effect soaked.
Embodiment 1
New multi-direction organizational project porous material irrigates system, also includes the second filling cavity joint 20, the second conduit 11
With the second negative pressure equipment 12, the other end of filling cavity 4 connects one end of the second conduit 11 by the second filling cavity joint 20, and second
Negative pressure equipment 12 makes nutrient solution in reservoir 1 be flowed in the second conduit 11 by filling cavity 4 by the first conduit 2, reaches group
The effect that weaver's journey porous material 8 laterally soaks.
Embodiment 2
New multi-direction organizational project porous material irrigates system, also includes the 3rd conduit 13, and the first negative pressure equipment 6 is the
One peristaltic pump, the first peristaltic pump comprises the first inlet 14 and the first liquid outlet 15, and first inlet 14 of the first peristaltic pump is even
The other end of logical negative pressure catheter 7, the first liquid outlet 15 connects one end of the 3rd conduit 13, the other end connection battalion of the 3rd conduit 13
Nutrient solution container 1.
First peristaltic pump is multistage peristaltic pump；Using multistage pump, organizational project porous material can be adjusted in filling cavity
Bear the size of fluid pressure, and then control it to absorb the speed of nutrient solution in organizational project porous material.
Embodiment 3
New multi-direction organizational project porous material irrigates system, also includes the 4th conduit 16, the second negative pressure equipment 12 is
Second peristaltic pump, the second peristaltic pump comprises the second inlet 17 and the second liquid outlet 18, the second inlet 17 of the second peristaltic pump
Connect the other end of the second conduit 11, the second liquid outlet 18 connects one end of the 3rd conduit 13, the other end of the 3rd conduit 13 is even
Logical reservoir 1, reaches the effect of nutrient solution recycling.
Embodiment 4
New multi-direction organizational project porous material irrigates system, also includes returnable bottle 19, the other end of the second conduit 11
And/or the other end of negative pressure catheter 7 arranges returnable bottle 19, negative pressure catheter 7 is connected back by conduit 21 respectively with the second conduit 11
Receive bottle 19.
In filling cavity 4, organizational project porous material 8 is arranged on the first filling cavity joint 3 and the second filling cavity joint 20
Between.
In preferred embodiment：
Organizational project porous material 8 comprises first end positioned at filling cavity 4 one end and be located at filling cavity 4 other end the
Two ends, the first end of organizational project porous material 8 and the second end are close to filling cavity 4 inwall, outer wall, filling cavity respectively
On 4 one end and/or filling cavity 4 other end, the first filling cavity joint 3 and the second filling cavity joint 20 are many by organizational project respectively
The first end of Porous materials 8 and the second end connect filling cavity 4.
In preferred embodiment, the first filling cavity joint 3 and the second filling cavity joint 20 are many by organizational project respectively
The first end of Porous materials 8 and the second end clamping and/or threaded filling cavity 4.
First filling cavity joint 3 and the second filling cavity joint 20 comprise tapered pole respectively, the first filling cavity joint 3 and second
Filling cavity joint 20 connects filling cavity 4 by tapered pole respectively, reaches the effect conveniently connecting and being fastenedly connected.
The utility model, the first negative pressure equipment 6 and the second negative pressure equipment can be using identical negative pressure structure or different bearing
Laminated structure, all can reach the perfusion on different directions.
The above-mentioned description to embodiment is to be understood that for ease of those skilled in the art and use this reality
With new.Person skilled in the art obviously easily can make various modifications to these embodiments, and here is said
Bright General Principle is applied in other embodiment without through performing creative labour.Therefore, the utility model is not limited to
State embodiment, those skilled in the art are according to announcement of the present utility model, the improvement made without departing from the utility model category
All should be within protection domain of the present utility model with modification.
Claims (10)
1. a kind of new multi-direction organizational project porous material perfusion system it is characterised in that：Including reservoir, first lead
Pipe, the first filling cavity joint, filling cavity, outer tube, the first negative pressure equipment, negative pressure catheter and organizational project porous material, described
Reservoir connects one end of the first conduit, and the other end of described first conduit passes through the first filling cavity fittings filling cavity
One end, the sheathed outer tube in outside of described filling cavity, between the inside portion of described outer tube and the outside portion of filling cavity formed
One negative pressure cavity, the sidepiece of described filling cavity arranges multiple holes making negative pressure cavity and connecting inside filling cavity, and described negative pressure is led
One end connection negative pressure cavity of pipe, the other end arranges the first negative pressure equipment, and described first negative pressure equipment makes nutrition in reservoir
Liquid is flow in negative pressure catheter by the first conduit, filling cavity, hole and negative pressure cavity successively, setting organizational project porous in described filling cavity
Material.
2. new multi-direction organizational project porous material perfusion system according to claim 1 it is characterised in that：Also include
Second filling cavity joint, the second conduit and the second negative pressure equipment, the other end of described filling cavity passes through the second filling cavity joint even
One end of logical second conduit, described second negative pressure equipment makes nutrient solution in reservoir be flowed into by filling cavity by the first conduit
In second conduit.
3. new multi-direction organizational project porous material perfusion system according to claim 1 it is characterised in that：Also include
3rd conduit, described first negative pressure equipment is the first peristaltic pump, and described first peristaltic pump comprises the first inlet and first and goes out liquid
Mouthful, the first inlet of described first peristaltic pump connects the other end of negative pressure catheter, and the first liquid outlet connects the one of the 3rd conduit
End, the other end connection reservoir of described 3rd conduit.
4. new multi-direction organizational project porous material perfusion system according to claim 3 it is characterised in that：Described
One peristaltic pump is multistage peristaltic pump.
5. new multi-direction organizational project porous material perfusion system according to claim 2 it is characterised in that：Also include
4th conduit, described second negative pressure equipment is the second peristaltic pump, and described second peristaltic pump comprises the second inlet and second and goes out liquid
Mouthful, the second inlet of described second peristaltic pump connects the other end of the second conduit, and the second liquid outlet connects the one of the 3rd conduit
End, the other end connection reservoir of described 3rd conduit.
6. new multi-direction organizational project porous material perfusion system according to claim 2 it is characterised in that：Also include
Returnable bottle, the other end setting returnable bottle of the other end of described second conduit and/or negative pressure catheter, described negative pressure catheter and second
Conduit is respectively communicated with returnable bottle.
7., according to the arbitrary described new multi-direction organizational project porous material perfusion system of claim 1 to 6, its feature exists
In：In described filling cavity, organizational project porous material is arranged between the first filling cavity joint and the second filling cavity joint.
8., according to the arbitrary described new multi-direction organizational project porous material perfusion system of claim 1 to 6, its feature exists
In：Described organizational project porous material comprises first end and the second end being located at the filling cavity other end positioned at filling cavity one end
Portion, the first end of described organizational project porous material and the second end are close to perfusion cavity wall, outer wall, filling cavity one respectively
On end and/or the filling cavity other end, described first filling cavity joint and the second filling cavity joint pass through organizational project porous respectively
The first end of material and the second end connect filling cavity.
9. new multi-direction organizational project porous material perfusion system according to claim 8 it is characterised in that：Described
One filling cavity joint and the second filling cavity joint are respectively by first end and the second end clamping of organizational project porous material
And/or threaded filling cavity.
10. new multi-direction organizational project porous material perfusion system according to claim 8 it is characterised in that：Described
First filling cavity joint and the second filling cavity joint comprise tapered pole respectively, and described first filling cavity joint and the second filling cavity connect
Head connects filling cavity by tapered pole respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620555886.0U CN205924550U (en) | 2016-06-08 | 2016-06-08 | Novel multi-direction tissue engineering porous material perfusion system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620555886.0U CN205924550U (en) | 2016-06-08 | 2016-06-08 | Novel multi-direction tissue engineering porous material perfusion system |
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| Publication Number | Publication Date |
|---|---|
| CN205924550U true CN205924550U (en) | 2017-02-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620555886.0U Withdrawn - After Issue CN205924550U (en) | 2016-06-08 | 2016-06-08 | Novel multi-direction tissue engineering porous material perfusion system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106010966A (en) * | 2016-06-08 | 2016-10-12 | 中山大学附属第医院 | Multidirectional tissue engineering porous material perfusion system |
-
2016
- 2016-06-08 CN CN201620555886.0U patent/CN205924550U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106010966A (en) * | 2016-06-08 | 2016-10-12 | 中山大学附属第医院 | Multidirectional tissue engineering porous material perfusion system |
| CN106010966B (en) * | 2016-06-08 | 2019-03-15 | 中山大学附属第一医院 | Multidirectional tissue engineering porous material perfusion system |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170208 Effective date of abandoning: 20190315 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20170208 Effective date of abandoning: 20190315 |