CN205924549U - Novel tissue engineering porous material perfusion system - Google Patents
Novel tissue engineering porous material perfusion system Download PDFInfo
- Publication number
- CN205924549U CN205924549U CN201620555025.2U CN201620555025U CN205924549U CN 205924549 U CN205924549 U CN 205924549U CN 201620555025 U CN201620555025 U CN 201620555025U CN 205924549 U CN205924549 U CN 205924549U
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- China
- Prior art keywords
- filling cavity
- conduit
- porous material
- tissue engineering
- nutrient solution
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 239000011148 porous material Substances 0.000 title claims abstract description 52
- 230000010412 perfusion Effects 0.000 title claims abstract description 21
- 235000015097 nutrients Nutrition 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims description 24
- 230000002572 peristaltic effect Effects 0.000 claims description 16
- 239000000243 solution Substances 0.000 description 21
- 210000001519 tissue Anatomy 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- -1 ECM) Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 102000007072 Nerve Growth Factors Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000003900 neurotrophic factor Substances 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000033667 organ regeneration Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a novel tissue engineering porous material system of infusing, the one end of nutrient solution container intercommunication first pipe, the other end of first pipe connect the one end that the intercommunication perfused the chamber through first chamber of infusing, and the other end of perfusing the chamber connects the one end of intercommunication second pipe through the second, and negative pressure equipment makes nutrient solution in the nutrient solution container flow in the second pipe through the chamber of perfusing by first pipe, and it sets up tissue engineering porous material to fill the intracavity. The utility model arranges the porous material of tissue engineering in the perfusion cavity, and the nutrient solution flows in the perfusion cavity through the negative pressure equipment, thereby realizing the pressure contact between the porous material of tissue engineering and the nutrient solution, and further greatly improving the speed and the uniformity of the porous material of tissue engineering for absorbing the nutrient solution; the multistage pump is adopted, so that the liquid bearing size of the tissue engineering porous material in the perfusion cavity can be adjusted, and the speed of the tissue engineering porous material for absorbing nutrient solution is controlled.
Description
Technical field
The present invention relates to tissue engineering technique field, a kind of specifically novel tissue engineering stephanoporate material perfusion system
System.
Background technology
Build bio-imitability organizational project reparation/graft materials with organizational project principle and carry out tissue/organ Regeneration and Repair
It is current study hotspot.Its general principle is：First pass through the complex arts such as physics, chemistry, biology preparation have structure imitate
Tissue engineering bracket/the repair materials of natural disposition, usually porous material；Secondly being dipped to porous material can promotion organization regeneration
Nutrient solution in, such as some extracellular matrixs (extracellular matrix, ECM), neurotrophic factor, medicine
Or the solution of stem cell or mixed solution, make organizational project porous material obtain biological activity bionic；Finally will be obtained bionical
Property tissue engineering material is implanted to defect/injured tissue site and carries out tissue repair, or by obtained bio-imitability tissue engineering material
After plantation target cell, after carrying out cell culture certain time in vitro, it is implanted to defect/injured tissue site again.
In prior art, organizational project porous material is carried out with nutriment load or the method for cell seeding mainly will
Organizational project porous material is immersed in nutrient solution and carries out nature absorption carriage.Using this kind of mode, organizational project is many
Porous materials and nutrient solution, mainly act on by passive adsorption completing load it is contemplated that various organizational project porous material
The pore size of material differs, differing property of shape, this mode of loading, is typically difficult to make nutriment fully, uniformly, effectively bear
It is loaded in organizational project porous material, and loading speed is slow, does not reach nutriment load effect.
It is accordingly required in particular to a kind of nutriment or Cell infusion novel tissue engineering stephanoporate material perfusion system, to solve
Certainly problems of the prior art.
Content of the invention
The invention aims in solution prior art, organizational project porous material culture speed is slow and uneven
Defect, provides a kind of novel tissue engineering stephanoporate material perfusion system, to solve problems of the prior art.
For achieving the above object, technical scheme is as follows：
A kind of novel tissue engineering stephanoporate material irrigates system, connects including reservoir, the first conduit, the first filling cavity
Head, filling cavity, the second filling cavity joint, the second conduit, negative pressure equipment and organizational project porous material, described reservoir is even
One end of logical first conduit, the other end of described first conduit passes through one end of the first filling cavity fittings filling cavity, described
The other end of filling cavity passes through one end of second filling cavity fittings the second conduit, and described negative pressure equipment makes in reservoir
Nutrient solution flows in the second conduit by the first conduit by filling cavity, setting organizational project porous material in described filling cavity.
In the present invention, also include returnable bottle, the other end of described second conduit is provided for making battalion in reservoir
Nutrient solution is flowed into the negative pressure equipment in the second conduit by the first conduit by filling cavity, and described second conduit connects returnable bottle, described
The negative pressure of negative pressure equipment makes the nutrient solution in reservoir pass sequentially through the first conduit, filling cavity, the second conduit inflow recovery
In bottle.
In the present invention, described negative pressure equipment is pump, and described pump is arranged on the second conduit.
In the present invention, described pump is multistage pump.
In the present invention, also include the 3rd conduit, described negative pressure equipment be peristaltic pump, described peristaltic pump comprise inlet and
Liquid outlet, the inlet of described peristaltic pump connects the other end of the second conduit, and liquid outlet connects one end of the 3rd conduit, and described the
The other end connection reservoir of three conduits.
In the present invention, described peristaltic pump is multistage peristaltic pump.
In the present invention, in described filling cavity, organizational project porous material is arranged on the first filling cavity joint and second
Between filling cavity joint.
In the present invention, described organizational project porous material comprises the first end positioned at filling cavity one end and is located at perfusion
The second end of the chamber other end, the first end of described organizational project porous material and the second end are close in filling cavity respectively
On wall, outer wall, filling cavity one end and/or the filling cavity other end, described first filling cavity joint and the second filling cavity joint are respectively
Filling cavity is connected by the first end and the second end of organizational project porous material.
In the present invention, described first filling cavity joint and the second filling cavity joint pass through organizational project porous material respectively
First end and the second end clamping and/or threaded filling cavity.
In the present invention, described first filling cavity joint and the second filling cavity joint comprise tapered pole respectively, and described first
Filling cavity joint and the second filling cavity joint connect filling cavity by tapered pole respectively.
Beneficial effect
1st, organizational project porous material is arranged in filling cavity the present invention, and nutrient solution is flowed in perfusion by negative pressure equipment
In chamber, realize the pressure contact between organizational project porous material and nutrient solution, and then greatly improve organizational project porous material
Absorb speed and the uniformity of nutrient solution；
2nd, adopt multistage pump, the size that organizational project porous material bears liquid in filling cavity, Jin Er can be adjusted
It is controlled to absorb the speed of nutrient solution in organizational project porous material.
3rd, in preferred embodiment, the first filling cavity joint and the second filling cavity joint connect filling by tapered pole respectively
Note chamber, reaches the effect conveniently connecting and being fastenedly connected.
Brief description
Fig. 1 the structural representation of present invention；
Fig. 2 present invention arranges structural representation during returnable bottle；
Fig. 3 is the A portion structural representation of Fig. 1；
Fig. 4 present invention adopts structural representation during peristaltic pump；
Fig. 5 is the B portion structural representation of Fig. 4；
Fig. 6 is the stereogram of Fig. 4.
In figure：1st, reservoir；2nd, the first conduit；3rd, the first filling cavity joint；4th, filling cavity；5th, the second filling cavity connects
Head；6th, the second conduit；7th, negative pressure equipment；8th, organizational project porous material；9th, returnable bottle；10th, the 3rd conduit；11st, peristaltic pump；
12nd, inlet；13rd, liquid outlet；14th, nutrient solution.
Specific embodiment
In the view of whole accompanying drawings, corresponding reference symbol represents corresponding part.
A kind of novel tissue engineering stephanoporate material irrigates system, including reservoir 1, the first conduit 2, the first filling cavity
Joint 3, filling cavity 4, the second filling cavity joint 5, the second conduit 6, negative pressure equipment 7 and organizational project porous material 8.
Reservoir 1 connects one end of the first conduit 2, and the other end of the first conduit 2 passes through the first filling cavity joint 3 even
One end of logical filling cavity 4, the other end of filling cavity 4 connects one end of the second conduit 6 by the second filling cavity joint 5, and negative pressure sets
Standby 7 (negative pressure equipment 7 may be provided on the first conduit 2 or the second conduit 6) make in reservoir 1 nutrient solution 14 by the first conduit
2 flow in the second conduit 6 by filling cavity 4, setting organizational project porous material 8 in filling cavity 4, and the length of filling cavity 4 can root
Length customization according to organizational project porous material.
Nutrient solution is flowed in filling cavity 4 by negative pressure equipment 7, realizes between organizational project porous material 8 and nutrient solution
Pressure contact, and then greatly improve organizational project porous material 8 and absorb the speed of nutrient solution and the uniformity.
Embodiment 1
Novel tissue engineering stephanoporate material irrigates system, also includes returnable bottle 9, the other end of the second conduit 6 is provided for
Nutrient solution in reservoir 1 is made to be flowed into the negative pressure equipment 7 in the second conduit 6 by filling cavity 4 by the first conduit 2, second leads
Pipe 6 connects returnable bottle 9, and the negative pressure of negative pressure equipment 7 makes the nutrient solution in reservoir 1 pass sequentially through the first conduit 2, filling cavity
4th, the second conduit 6 flows in returnable bottle 9.
In preferred embodiment, negative pressure equipment 7 is pump (pump can be water pump, membrane pump or mechanical pump etc.), and pump is arranged
On the second conduit 6.
Pump can adopt multistage pump, can adjust the size that organizational project porous material bears fluid pressure in filling cavity,
And then control it to absorb the speed of nutrient solution in organizational project porous material.
Embodiment 2
Novel tissue engineering stephanoporate material irrigates system, also includes the 3rd conduit 10, and negative pressure equipment 7 is peristaltic pump 11, compacted
Dynamic pump 11 comprises inlet 12 and liquid outlet 13, and the inlet 12 of peristaltic pump 11 connects the other end of the second conduit 6, liquid outlet
One end of 13 connection the 3rd conduits 10, the other end connection reservoir 1 of the 3rd conduit 10.
Peristaltic pump 11 is multistage peristaltic pump, can adjust organizational project according to the growth cycle of organizational project porous material 8
Porous material 8 bears the size of liquid in filling cavity 4, and then controls it in organizational project porous material 8 different growth periods
Absorb the speed of nutrient solution.
In filling cavity 4, organizational project porous material 8 be arranged on the first filling cavity joint 3 and the second filling cavity joint 5 it
Between.
Organizational project porous material 8 comprises first end positioned at filling cavity 4 one end and be located at filling cavity 4 other end the
Two ends, the first end of organizational project porous material 8 and the second end are close to filling cavity 4 inwall, outer wall, filling cavity respectively
On 4 one end and/or filling cavity 4 other end, the first filling cavity joint 3 and the second filling cavity joint 5 are many by organizational project respectively
The first end of Porous materials 8 and the second end connect filling cavity 4.
In preferred embodiment, the first filling cavity joint 3 and the second filling cavity joint 5 pass through organizational project porous respectively
The first end of material 8 and the second end clamping and/or threaded filling cavity 4.
First filling cavity joint 3 and the second filling cavity joint 5 comprise tapered pole respectively, the first filling cavity joint 3 and second
Filling cavity joint 5 connects filling cavity 4 by tapered pole respectively, reaches the effect conveniently connecting and being fastenedly connected.
The above-mentioned description to embodiment is to be understood that for ease of those skilled in the art and use this
Bright.Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein
General Principle is applied in other embodiment without through performing creative labour.Therefore, the invention is not restricted to above-described embodiment,
, according to the announcement of the present invention, the improvement made without departing from scope and modification all should be at these for those skilled in the art
Within bright protection domain.
Claims (10)
1. a kind of novel tissue engineering stephanoporate material perfusion system it is characterised in that：Including reservoir, the first conduit,
One filling cavity joint, filling cavity, the second filling cavity joint, the second conduit, negative pressure equipment and organizational project porous material, described battalion
One end of nutrient solution reservoir first conduit, the other end of described first conduit passes through the first filling cavity fittings filling cavity
One end, the other end of described filling cavity passes through one end of second filling cavity fittings the second conduit, and described negative pressure equipment makes battalion
In nutrient solution container, nutrient solution flows in the second conduit by the first conduit by filling cavity, and in described filling cavity, setting organizational project is many
Porous materials.
2. novel tissue engineering stephanoporate material according to claim 1 perfusion system it is characterised in that：Also include reclaiming
Bottle, the other end of described second conduit is provided for making in reservoir nutrient solution to pass through filling cavity inflow the by the first conduit
Negative pressure equipment in two conduits, described second conduit connection returnable bottle, the negative pressure of described negative pressure equipment makes in reservoir
Nutrient solution pass sequentially through the first conduit, filling cavity, second conduit flow into returnable bottle in.
3. organizational project according to claim 2 many skies material perfusion system it is characterised in that：Described negative pressure equipment is
Pump, described pump is arranged on the second conduit.
4. organizational project according to claim 3 many skies material perfusion system it is characterised in that：Described pump is multistage pump.
5. novel tissue engineering stephanoporate material according to claim 1 perfusion system it is characterised in that：Also include the 3rd to lead
Pipe, described negative pressure equipment is peristaltic pump, and described peristaltic pump comprises inlet and liquid outlet, the inlet connection of described peristaltic pump the
The other end of two conduits, liquid outlet connects one end of the 3rd conduit, the other end connection reservoir of described 3rd conduit.
6. novel tissue engineering stephanoporate material according to claim 5 perfusion system it is characterised in that：Described peristaltic pump is
Multistage peristaltic pump.
7. according to the arbitrary described novel tissue engineering stephanoporate material perfusion system of claim 1 to 6 it is characterised in that：Institute
State in filling cavity, organizational project porous material is arranged between the first filling cavity joint and the second filling cavity joint.
8. according to the arbitrary described novel tissue engineering stephanoporate material perfusion system of claim 1 to 6 it is characterised in that：Described
Organizational project porous material comprises the first end and the second end being located at the filling cavity other end positioned at filling cavity one end, described
The first end of organizational project porous material and the second end be close to respectively perfusion cavity wall, outer wall, filling cavity one end and/or
On the filling cavity other end, described first filling cavity joint and the second filling cavity joint pass through the of organizational project porous material respectively
One end and the second end connect filling cavity.
9. novel tissue engineering stephanoporate material according to claim 8 perfusion system it is characterised in that：Described first perfusion
Chamber joint and the second filling cavity joint are respectively by first end and the second end clamping and/or the spiral shell of organizational project porous material
Line connects filling cavity.
10. novel tissue engineering stephanoporate material according to claim 8 perfusion system it is characterised in that：Described first filling
Note chamber joint and the second filling cavity joint comprise tapered pole respectively, and described first filling cavity joint and the second filling cavity joint are respectively
Filling cavity is connected by tapered pole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620555025.2U CN205924549U (en) | 2016-06-08 | 2016-06-08 | Novel tissue engineering porous material perfusion system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620555025.2U CN205924549U (en) | 2016-06-08 | 2016-06-08 | Novel tissue engineering porous material perfusion system |
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| Publication Number | Publication Date |
|---|---|
| CN205924549U true CN205924549U (en) | 2017-02-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620555025.2U Expired - Fee Related CN205924549U (en) | 2016-06-08 | 2016-06-08 | Novel tissue engineering porous material perfusion system |
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| Country | Link |
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| CN (1) | CN205924549U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105963794A (en) * | 2016-06-08 | 2016-09-28 | 中山大学附属第医院 | Tissue engineering porous material perfusion system |
| CN108490854A (en) * | 2018-03-19 | 2018-09-04 | 哈尔滨市第医院 | A kind of control system in multi-direction organizational project porous material perfusion |
-
2016
- 2016-06-08 CN CN201620555025.2U patent/CN205924549U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105963794A (en) * | 2016-06-08 | 2016-09-28 | 中山大学附属第医院 | Tissue engineering porous material perfusion system |
| CN108490854A (en) * | 2018-03-19 | 2018-09-04 | 哈尔滨市第医院 | A kind of control system in multi-direction organizational project porous material perfusion |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170208 Termination date: 20210608 |
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| CF01 | Termination of patent right due to non-payment of annual fee |