CN106010966B - System is perfused in multi-direction organizational project porous material - Google Patents
System is perfused in multi-direction organizational project porous material Download PDFInfo
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- CN106010966B CN106010966B CN201610404357.5A CN201610404357A CN106010966B CN 106010966 B CN106010966 B CN 106010966B CN 201610404357 A CN201610404357 A CN 201610404357A CN 106010966 B CN106010966 B CN 106010966B
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- filling cavity
- porous material
- organizational project
- negative pressure
- conduit
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
Abstract
The present invention discloses a kind of multi-direction organizational project porous material perfusion system, including reservoir, the first conduit, the first filling cavity connector, filling cavity, outer tube, the first negative pressure equipment, negative pressure catheter and organizational project porous material.Organizational project porous material is arranged in filling cavity the present invention, nutrient solution is flowed in filling cavity by negative pressure equipment, it realizes the pressure contact between organizational project porous material and nutrient solution, and then greatly improves speed and the uniformity that organizational project porous material absorbs nutrient solution；Using multistage pump, the size that organizational project porous material bears liquid in filling cavity can be adjusted, and then controls its speed for absorbing nutrient solution in organizational project porous material.
Description
Technical field
The present invention relates to tissue engineering technique field, a kind of specifically multi-direction organizational project porous material perfusion is
System.
Background technique
Tissue/organ Regeneration and Repair is carried out with organizational project principle building bio-imitability organizational project reparation/graft materials
It is current research hotspot.Its basic principle are as follows: imitative with structure by the preparation of the complex arts such as physics, chemistry, biology first
Tissue engineering bracket/repair materials of natural disposition, usually porous material；Secondly porous material is dipped to can promote regeneration
Nutrient solution in, such as certain extracellular matrixs (extracellular matrix, ECM), neurotrophic factor, drug
Or the solution or mixed solution of stem cell, so that organizational project porous material is obtained biological activity bionic；It finally will be obtained bionical
Property tissue engineering material be implanted to defect/injured tissue site and carry out tissue repair, or by obtained bio-imitability tissue engineering material
After planting target cell, cell culture is carried out in vitro and is implanted to defect/injured tissue site again after a certain period of time.
In the prior art, the method for carrying out nutriment load or cell seeding to organizational project porous material mainly will
Organizational project porous material is immersed in progress nature absorption carriage in nutrient solution.Using such mode, organizational project is more
Porous materials and nutrient solution are mainly completed to load by passive adsorption effect, it is contemplated that the various porous materials of organizational project
The pore size of material is different, the different property of shape, this mode of loading, is typically difficult to bear nutriment sufficiently, uniformly, effectively
It is loaded in organizational project porous material, and loading speed is slow, nutriment load effect is not achieved.Especially for containing longitudinal hole
The porous ability of organizational project in channel and lateral hole, load efficiency, effect are worse.
It is accordingly required in particular to system is perfused in a kind of nutriment or Cell infusion organizational project porous material, it is existing to solve
There is the problem of technology.
Summary of the invention
The purpose of the present invention is to solve in the prior art, organizational project porous material culture speed is slowly and non-uniform
Defect provides a kind of multi-direction organizational project porous material perfusion system, to solve problems of the prior art.
To achieve the above object, technical scheme is as follows:
A kind of multi-direction organizational project porous material perfusion system, including reservoir, the first conduit, the first filling cavity
Connector, filling cavity, outer tube, the first negative pressure equipment, negative pressure catheter and organizational project porous material, the reservoir connection
One end of first conduit, the other end of first conduit pass through one end of the first filling cavity fittings filling cavity, the filling
It is arranged outer tube on the outside of note chamber, forms a negative pressure cavity, institute between the inside portion of the outer tube and the outside portion of filling cavity
It states and multiple holes for making to be connected to inside negative pressure cavity and filling cavity is set on the side of filling cavity, one end connection of the negative pressure catheter is negative
Chamber is pressed, the first negative pressure equipment is arranged in the other end, and first negative pressure equipment leads nutrient solution in reservoir successively by first
Pipe, filling cavity, hole and negative pressure cavity are flow in negative pressure catheter, and organizational project porous material is arranged in the filling cavity.
In the present invention, further include the second filling cavity connector, the second conduit and the second negative pressure equipment, the filling cavity it is another
One end passes through one end of second the second conduit of filling cavity fittings, and second negative pressure equipment makes nutrient solution in reservoir
It is flowed into the second conduit by the first conduit by filling cavity.
It in the present invention, further include third conduit, first negative pressure equipment is the first peristaltic pump, first peristaltic pump
Comprising the first inlet and the first liquid outlet, the other end of the first inlet connection negative pressure catheter of first peristaltic pump, the
One liquid outlet is connected to one end of third conduit, and the other end of the third conduit is connected to reservoir.
In the present invention, first peristaltic pump is multistage peristaltic pump.
It in the present invention, further include the 4th conduit, second negative pressure equipment is the second peristaltic pump, second peristaltic pump
Comprising the second inlet and the second liquid outlet, the second inlet of second peristaltic pump is connected to the other end of the second conduit, the
Two liquid outlets are connected to one end of the 4th conduit, and the other end of the 4th conduit is connected to reservoir.
It in the present invention, further include returnable bottle, the other end setting of the other end and/or negative pressure catheter of second conduit
Returnable bottle, the negative pressure catheter and the second conduit are respectively communicated with returnable bottle.
In the present invention, in the filling cavity, organizational project porous material is arranged in the first filling cavity connector and second
Between filling cavity connector.
In the present invention, the organizational project porous material includes positioned at the first end of filling cavity one end and positioned at perfusion
The second end of the chamber other end, the first end and the second end of the organizational project porous material are tightly attached in filling cavity respectively
On wall, the first filling cavity connector and the second filling cavity connector pass through the first end and of organizational project porous material respectively
Two ends connect filling cavity.
In the present invention, the first filling cavity connector and the second filling cavity connector pass through organizational project porous material respectively
First end and the second end clamping and/or be threadedly coupled filling cavity.
In the present invention, the first filling cavity connector and the second filling cavity connector separately include tapered pole, and described first
Filling cavity connector and the second filling cavity connector pass through tapered pole respectively and connect filling cavity.
Beneficial effect
1, organizational project porous material is arranged in filling cavity the present invention, and nutrient solution is being perfused by negative pressure equipment flowing
It is intracavitary, realize the pressure contact between organizational project porous material and nutrient solution, and then greatly improve organizational project porous material
Absorb the speed and the uniformity of nutrient solution；
2, multiple holes on the side of filling cavity permeate nutrient solution through hole, reach organizational project porous material and longitudinally soak
The effect of bubble；
3, using multistage pump, the size that organizational project porous material bears liquid in filling cavity, Jin Er can be adjusted
Its speed for absorbing nutrient solution is controlled in organizational project porous material.
Detailed description of the invention
Fig. 1 the structural representation of present invention；
Fig. 2 is the structural schematic diagram in the portion A of Fig. 1；
The structural schematic diagram for the organizational project porous material being arranged in Fig. 3 filling cavity of the present invention；
Fig. 4 is the perspective view of Fig. 1；
Fig. 5 present invention uses schematic diagram when peristaltic pump structure；
Schematic diagram when Fig. 6 present invention is using recycling bottle structure.
In figure: 1, reservoir；2, the first conduit；3, the first filling cavity connector；4, filling cavity；5, outer tube；6,
One negative pressure equipment；7, negative pressure catheter；8, organizational project porous material；9, negative pressure cavity；10, hole；11, the second conduit；12, second is negative
Press equipment；13, third conduit；14, the first inlet；15, the first liquid outlet；16, the 4th conduit；17, the second inlet；18,
Second liquid outlet；19, returnable bottle；20, the second filling cavity connector.
Specific embodiment
In the view of whole attached drawings, corresponding reference symbol indicates corresponding component.
A kind of multi-direction organizational project porous material perfusion system, including the perfusion of reservoir 1, the first conduit 2, first
Chamber connector 3, filling cavity 4, outer tube 5, the first negative pressure equipment 6, negative pressure catheter 7 and organizational project porous material 8.
Reservoir 1 is connected to one end of the first conduit 2, and the other end of the first conduit 2 is connected by the first filling cavity connector 3
One end of logical filling cavity 4, the outside of filling cavity 4 is arranged outer tube 5, between the inside portion of outer tube 5 and the outside portion of filling cavity 4
A negative pressure cavity 9 is formed, multiple holes 10 for making to be connected to inside negative pressure cavity 9 and filling cavity 4, negative pressure are set on the side of filling cavity 4
One end of conduit 7 is connected to negative pressure cavity 9, and the first negative pressure equipment 6 is arranged in the other end, and the first negative pressure equipment 6 makes battalion in reservoir 1
Nutrient solution is successively flow in negative pressure catheter 7 by the first conduit 2, filling cavity 4, hole 10 and negative pressure cavity 9, setting group weaver in filling cavity 4
Journey porous material 8.
Organizational project porous material 8 is arranged in filling cavity 4 present invention, and nutrient solution is being perfused by negative pressure equipment flowing
In chamber 4, the pressure contact between organizational project porous material 8 and nutrient solution is realized, and then greatly improve the porous material of organizational project
Material 8 absorbs the speed and the uniformity of nutrient solution；
Multiple holes 10 on the side of filling cavity 4 permeate nutrient solution through hole 10, and it is vertical to reach organizational project porous material 8
To the effect of immersion.
Embodiment 1
System is perfused in multi-direction organizational project porous material, further includes the second filling cavity connector 20, the second conduit 11 and the
Two negative pressure equipments 12, the other end of filling cavity 4 are connected to one end of the second conduit 11, the second negative pressure by the second filling cavity connector 20
Equipment 12 flows into nutrient solution in reservoir 1 in second conduit 11 by filling cavity 4 by the first conduit 2, reaches a group weaver
The effect that journey porous material 8 laterally impregnates.
Embodiment 2
System is perfused in multi-direction organizational project porous material, further includes third conduit 13, and the first negative pressure equipment 6 is first compacted
Dynamic pump, the first peristaltic pump include the first inlet 14 and the first liquid outlet 15, and the first inlet 14 connection of the first peristaltic pump is negative
The other end of conduit 7 is pressed, the first liquid outlet 15 is connected to one end of third conduit 13, and the other end of third conduit 13 is connected to nutrient solution
Container 1.
First peristaltic pump is multistage peristaltic pump；Using multistage pump and multistage peristaltic pump structure structure, a group weaver can be adjusted
Journey porous material bears the size of fluid pressure in filling cavity, and then controls it in organizational project porous material and absorb nutrition
The speed of liquid.
Embodiment 3
System is perfused in multi-direction organizational project porous material, further includes the 4th conduit 16, and the second negative pressure equipment 12 is second
Peristaltic pump, the second peristaltic pump include the second inlet 17 and the second liquid outlet 18, and the second inlet 17 of the second peristaltic pump is connected to
The other end of second conduit 11, the second liquid outlet 18 are connected to one end of the 4th conduit 16, and the other end of the 4th conduit 16 is connected to battalion
Nutrient solution container 1 achievees the effect that nutrient solution is recycled.
Embodiment 4
System is perfused in multi-direction organizational project porous material, further includes returnable bottle 19, the other end of the second conduit 11 and/or
Returnable bottle 19 is arranged in the other end of negative pressure catheter 7, and negative pressure catheter 7 is connected to returnable bottle by conduit 21 respectively with the second conduit 11
19。
In filling cavity 4, organizational project porous material 8 is arranged in the first filling cavity connector 3 and the second filling cavity connector 20
Between.
In preferred embodiment:
Organizational project porous material 8 includes the first end positioned at 4 one end of filling cavity and the positioned at 4 other end of filling cavity
Two ends, the first end and the second end of organizational project porous material 8 are tightly attached to 4 inner wall of filling cavity, outer wall, filling cavity respectively
On 4 other end of 4 one end and/or filling cavity, the first filling cavity connector 3 and the second filling cavity connector 20 are more by organizational project respectively
The first end of Porous materials 8 connects filling cavity 4 with the second end.
In preferred embodiment, the first filling cavity connector 3 and the second filling cavity connector 20 are more by organizational project respectively
The first end and the second end of Porous materials 8 are clamped and/or are threadedly coupled filling cavity 4.
First filling cavity connector 3 and the second filling cavity connector 20 separately include tapered pole, the first filling cavity connector 3 and second
Filling cavity connector 20 connects filling cavity 4 by tapered pole respectively, achievees the effect that facilitate connection and be fastenedly connected.
Identical negative pressure structure or different negative pressure knots can be used in the present invention, the first negative pressure equipment 6 and the second negative pressure equipment
Structure all can reach the perfusion on different directions.
This hair can be understood and used the above description of the embodiments is intended to facilitate those skilled in the art
It is bright.Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein
General Principle is applied in other embodiments without having to go through creative labor.Therefore, the present invention is not limited to the above embodiments,
Those skilled in the art's announcement according to the present invention, improvement and modification made without departing from the scope of the present invention all should be in this hairs
Within bright protection scope.
Claims (10)
1. system is perfused in a kind of multi-direction organizational project porous material, it is characterised in that: including reservoir, the first conduit,
First filling cavity connector, filling cavity, outer tube, the first negative pressure equipment, negative pressure catheter and organizational project porous material, the nutrition
Liquid container is connected to one end of the first conduit, and the other end of first conduit passes through the one of the first filling cavity fittings filling cavity
It holds, is arranged outer tube on the outside of the filling cavity, form one between the inside portion of the outer tube and the outside portion of filling cavity
Multiple holes for making to be connected to inside negative pressure cavity and filling cavity are arranged on the side of the filling cavity for negative pressure cavity, the negative pressure catheter
One end is connected to negative pressure cavity, and the other end is arranged the first negative pressure equipment, first negative pressure equipment make in reservoir nutrient solution according to
It is secondary to be flow in negative pressure catheter by the first conduit, filling cavity, hole and negative pressure cavity, organizational project porous material is set in the filling cavity
Material.
2. system is perfused in multi-direction organizational project porous material according to claim 1, it is characterised in that: further include second
Filling cavity connector, the second conduit and the second negative pressure equipment, the other end of the filling cavity pass through the second filling cavity fittings the
One end of two conduits, second negative pressure equipment make in reservoir nutrient solution pass through filling cavity by the first conduit to flow into second
In conduit.
3. system is perfused in multi-direction organizational project porous material according to claim 1, it is characterised in that: further include third
Conduit, first negative pressure equipment are the first peristaltic pump, and first peristaltic pump includes the first inlet and the first liquid outlet, institute
The other end of the first inlet connection negative pressure catheter of the first peristaltic pump is stated, the first liquid outlet is connected to one end of third conduit, institute
State the other end connection reservoir of third conduit.
4. system is perfused in multi-direction organizational project porous material according to claim 3, it is characterised in that: described first is compacted
Dynamic pump is multistage peristaltic pump.
5. system is perfused in multi-direction organizational project porous material according to claim 2, it is characterised in that: further include the 4th
Conduit, second negative pressure equipment are the second peristaltic pump, and second peristaltic pump includes the second inlet and the second liquid outlet, institute
The second inlet for stating the second peristaltic pump is connected to the other end of the second conduit, and the second liquid outlet is connected to one end of the 4th conduit, institute
State the other end connection reservoir of the 4th conduit.
6. system is perfused in multi-direction organizational project porous material according to claim 2, it is characterised in that: further include recycling
Returnable bottle, the negative pressure catheter and the second conduit is arranged in bottle, the other end of second conduit and/or the other end of negative pressure catheter
It is respectively communicated with returnable bottle.
7. system is perfused in multi-direction organizational project porous material according to any one of claims 1 to 6, it is characterised in that:
In the filling cavity, organizational project porous material is arranged between the first filling cavity connector and the second filling cavity connector.
8. system is perfused in multi-direction organizational project porous material according to any one of claims 1 to 6, it is characterised in that: institute
Stating organizational project porous material includes the second end positioned at the first end of filling cavity one end and positioned at the filling cavity other end, institute
The first end and the second end for stating organizational project porous material are tightly attached to respectively on perfusion cavity wall, and first filling cavity connects
The first end that head passes through organizational project porous material with the second filling cavity connector respectively connects filling cavity with the second end.
9. system is perfused in multi-direction organizational project porous material according to claim 8, it is characterised in that: described first fills
Note chamber connector and the second filling cavity connector pass through respectively organizational project porous material first end and the second end clamping and/or
It is threadedly coupled filling cavity.
10. system is perfused in multi-direction organizational project porous material according to claim 8, it is characterised in that: described first
Filling cavity connector and the second filling cavity connector separately include tapered pole, the first filling cavity connector and the second filling cavity connector point
Filling cavity is not connected by tapered pole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610404357.5A CN106010966B (en) | 2016-06-08 | 2016-06-08 | System is perfused in multi-direction organizational project porous material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610404357.5A CN106010966B (en) | 2016-06-08 | 2016-06-08 | System is perfused in multi-direction organizational project porous material |
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| Publication Number | Publication Date |
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| CN106010966A CN106010966A (en) | 2016-10-12 |
| CN106010966B true CN106010966B (en) | 2019-03-15 |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108085254A (en) * | 2018-02-09 | 2018-05-29 | 中国人民解放军第四军医大学 | Tissue engineering product culture apparatus |
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