CN2397122Y - Rotary tube cell/tissue culture device - Google Patents
Rotary tube cell/tissue culture device Download PDFInfo
- Publication number
- CN2397122Y CN2397122Y CN99244872U CN99244872U CN2397122Y CN 2397122 Y CN2397122 Y CN 2397122Y CN 99244872 U CN99244872 U CN 99244872U CN 99244872 U CN99244872 U CN 99244872U CN 2397122 Y CN2397122 Y CN 2397122Y
- Authority
- CN
- China
- Prior art keywords
- fixed
- motor
- tissue culture
- air
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
Abstract
The utility model relates to a rotating tube cell/tissue culture device which comprises one pair of brackets for supporting the rotating tube cell/tissue culture device and a motor, wherein the brackets and the motor are fixed on a bottom seat, and another motor is fixed on the brackets; a composite inner cylinder which is composed of gas and liquid exchange membranes is fixed on a hollowed cylinder which is arranged on the center of a cell culture chamber, both ends of the inner cylinder are fixed on the brackets through a rotating shaft, and the rotating shaft of one end is connected with a rotating mechanism which is composed of a motor and a speed changing mechanism; the bottom end of the inner cylinder is connected with an isolating mechanism; a hollow bolt is arranged an another end plate of the main body of the rotating tube cell/tissue culture device, and a movable valve is pressed downwards and fixed on the hollow bolt; a gas system and a liquid system are connected with a gas inlet and a liquid inlet of the rotating tube cell/tissue culture device.
Description
The utility model relates to the biomedical tissue engineering, particularly relates to a kind of swivel pipe cell/tissue dimensional culture device in the organizational project.
A kind of utility unit roll cell culture apparatus (RCCS) of simplification has been developed at NASA (NASA) Johnson center on the basis of rotary biological tissue incubator (RWV), at document [1] Culturing a Future, Fall, 1998, introduction is arranged among the MicrogravityNews, see accompanying drawing 1.This device is characterized in seeing through the online air feed of gas/liquid exchange membrane by the inner core of coaxial rotation by the inside and outside tube that has a support to be used for to support incubator on the base, upgrades nutrient solution by the end spout during shutdown.Experience shows, pair cell/tissue culture, the oxygen supply is more more urgent than nutritive substance supply, big and this incubator of the consumption of oxygen can only online air feed can not oxygen supply, unfavorable for growth the cultivation of some tissue (as tumor tissues etc.).After cell aggregation formed and links a dimensioning, it is not necessarily big that short period of time sedimentation and contact with wall influences.For the cultivation of cell one degradation material matrix framework aggregate, situation also is like this.And this simplification device is easy and simple to handle, and whole culturing process is visual, is suitable for being used for tumor tissues drug toxicology test etc.But this class incubator has only the rotation of a direction, cell settlement is arranged easily cultivating on the locular wall, is unfavorable for real dimensional culture.
The utility model purpose is: the incubator that overcomes prior art has only the inside and outside tube of single stent support incubator, and causes unsettled shortcoming in the rotation process, and shutdown adds the shortcoming that nutrient solution causes the growth of cell settlement supression cell; In order to simplify the incubator structure, be convenient to operation, visual in whole culturing process; Thereby provide a kind of inside and outside rotary tube of incubator not only can to rotate synchronously but also can differentially rotate, be beneficial to Rotary pipe type cell/tissue three-dimensional incubator that the regulation and control planted agent of culturing room distributes.
The purpose of this utility model is achieved in that
Rotary pipe type cell/tissue three-dimensional incubator that the utility model provides, comprise that the two-layer cylinder concentric locking collar of cell culture chamber and air chamber dresses up the incubator main body that one constitutes, be fixed with a pair of support and motor that is used to support the incubator main body on one base, another motor is fixed on the support, it is characterized in that: comprise that also 2 motors electrically connect the rotating mechanism of composition respectively with 2 speed change mechanisms, center at cell culture chamber has-fix the compound inner core that a gas/liquid exchange membrane constitutes on the tube of hollow out garden, inner core is an air chamber, its two ends are fixed on the support by rotating shaft, wherein an end rotating shaft is connected with rotating mechanism, the inner core end links to each other with isolation mechanism, there is a gas to advance on the isolation mechanism, outlet, one sealing-ring is arranged between it, 3 hollow bolts are arranged on another end plate of incubator main body, press solidly a Movable valve under the hollow bolt, be convenient to change nutrient solution; When the needs liquid feeding, the liquid fillers of the tubing in liquid-supplying system liquid feeding in Movable valve, the liquid pipe of the infusion pump in the liquid-supplying system links to each other with the nutrient solution memory, after passing deoscillator earlier by the perforated tube pipeline again, enter cell culture chamber to terminal, the output channel of cell culture chamber is connected in the nutrient solution dialyzer mutually, and it communicates with nutrient solution withdrawer and meta-bolites collector respectively; Be connected air inlet of air chamber in the airing system on the incubator inlet mouth and source of the gas by the voltage stabilizing on the tracheae, relief valve connects; The air outlet of air chamber is connected on the residual air collector by vapor pipe.
Advantage of the present utility model:
1, the inside and outside tube of culturing room both can rotate synchronously, can differentially rotate again; Rotate synchronously and make in the culturing room shearing force very low, be beneficial to cell aggregation, adhesion; Stress of fluidity in the adjustable culturing room of differential rotation is broken up with inducing cell; (1) the rotating shaft double support supports incubator main body, trembling in the time of can avoiding speed of rotation higher
Moving, in order to avoid therefore influence the cell/tissue dimensional culture, improved spin stabilization
The property.(2) this incubator is compared with other dimensional culture device, has lacked a ring-shaped air chamber, in
Tube changes air supply channel into, and this structure brings whole culturing process visual.(3) this incubator is simple in structure, and is easy to operate.Below in conjunction with drawings and Examples the utility model is described in detail;
Fig. 1 is that the utility model structural representation Fig. 2 is that airing system block diagram 3 of the present utility model is the utility model liquid-supplying system skeleton diagrams
Embodiment 1
Manufacture a Rotary pipe type cell/tissue three-dimensional incubator by Fig. 1-3, the air chamber outside surface Φ 1000mm of this incubator; Internal surface Φ 80mm * long 60mm; Air chamber outside surface Φ 25mm, Φ
In12mm; The incubator main body is made with polycarbonate material, and the hollow rotation axis is made with stainless steel or fluorinated ethylene propylene material, the helmet cancer cells BGC-823 of cultivator, and the microballon carrier is Cytodcx-3, nutrient solution sample and method are carried out according to a conventional method.This incubator comprises in Fig. 2: (1) cell/tissue culturing room, and its inwall is gas/liquid exchange membrane-hollow out support composite structure (2); (2) be gas/liquid exchange membrane one hollow out support composite structure, it and inner core rotating shaft (4) link; (3) air chamber, i.e. hollow rotating inner core; (4) inner core rotating shaft, it links to each other with inner core speed change mechanism (6); (5) urceolus rotation speed change mechanism links to each other with motor (7); (6) inner core rotation speed change mechanism links to each other with motor (8); (7), (8) commercially available torque motor; (9), (10) be respectively inlet mouth and air outlet, link to each other with gas circuit; 11) be stainless steel stent, be used to support the incubator main body; 12) be the stainless steel base, stainless steel stent is fixed thereon; 13) isolation mechanism; Inlet mouth (9) and air outlet (10) are not rotated with axle, and import and outlet are kept apart; Nutrient solution transport system wherein, as shown in Figure 3, in the drawings: (18) are bright nutrient solution memory; (19) be the infusion pump of flow, adjustable in pressure; (20) for the perforated tube pipeline of nutrient solution input usefulness, enter culturing room to culturing room terminal through deoscillator.Deoscillator is in order to regulate culturing room's internal pressure (Pb (ζ)); (22) be the nutrient solution dialyzer; (23) be the nutrient solution withdrawer; (24) be the meta-bolites collector; Wherein the liquid pipe of infusion pump (19) links to each other with nutrient solution memory (18), after passing deoscillator (21) earlier by perforated tube pipeline (20) again, enter cell culture chamber to culturing room's terminal, the output channel of cell culture chamber links to each other and trains liquid dialyzer (22), and it communicates with nutrient solution withdrawer (23) and meta-bolites collector (24) respectively; Air feed---exhaust system wherein, as shown in Figure 8, in the drawings: (25) are source of the gas, and with tracheae and voltage stabilizing, relief valve links to each other, and air pipe outlet is connected to incubator air chamber (7);
(26) be voltage stabilizing, relief valve is installed among the carrier gas road;
(27) be that inlet pipe links to each other with incubator inlet mouth (9);
(28) be that vapor pipe links to each other with incubator air outlet (10);
(29) be the residual air collector; Wherein air inlet of air chamber and source of the gas (25) are by the voltage stabilizing on the tracheae, and relief valve (26) connects; The air chamber air outlet connects on the residual air collector (29) by vapor pipe (28).
Compare with the RCCS of the U.S., principal feature is that (1) inside and outside rotor (coaxial) can rotate (ω 1=ω 2=ω) synchronously, also can differentially rotate (ω 1 ≠ ω 2).During rotation synchronously, stress of fluidity is very low in the incubator, goes to zero, and helps cell aggregation, adhesion; During differential rotation, stress of fluidity distributes in the adjustable culturing room, is beneficial to the inducing cell differentiation, grows up to tissues needed.(2) two supports are arranged, incubator can not shaken when high speed rotating, thereby has improved the stability of instrument.
Claims (1)
1, a kind of Rotary pipe type cell/tissue culture device comprises that the incubator main body is fixed on the base with stent support, and motor is electrically connected with speed change mechanism, and motor is fixed on the base, links to each other with speed change mechanism by rotating shaft again; It is characterized in that: comprise that also a motor is fixed on another support, center at cell culture chamber has-hollow out garden tube on the compound inner core that constitutes of fixing-gas/liquid exchange membrane, the inner core two ends are fixed on the support by rotating shaft, wherein an end rotating shaft is connected with speed change mechanism, speed change mechanism is electrically connected with motor again, the end plate that the inner core end exposes the incubator main body links to each other with isolation mechanism, one air inlet is arranged on the isolation mechanism, the air outlet, one sealing-ring is arranged between it, 3 hollow bolts are arranged on another end plate of incubator main body, press solidly a Movable valve under the empty bolt, the liquid pipe of the infusion pump in the liquid-supplying system links to each other with the nutrient solution memory, after passing deoscillator earlier by the perforated tube pipeline again, enter cell culture chamber to terminal, the output channel of cell culture chamber is connected in the nutrient solution dialyzer mutually, and it communicates with nutrient solution withdrawer and meta-bolites collector respectively; Air inlet of air chamber in the airing system and source of the gas are by the voltage stabilizing on the tracheae, and relief valve connects; The air outlet of air chamber is connected on the residual air collector by vapor pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99244872U CN2397122Y (en) | 1999-09-20 | 1999-09-20 | Rotary tube cell/tissue culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99244872U CN2397122Y (en) | 1999-09-20 | 1999-09-20 | Rotary tube cell/tissue culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2397122Y true CN2397122Y (en) | 2000-09-20 |
Family
ID=34030876
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99244872U Expired - Fee Related CN2397122Y (en) | 1999-09-20 | 1999-09-20 | Rotary tube cell/tissue culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2397122Y (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199537A (en) * | 2011-04-13 | 2011-09-28 | 北京理工大学 | Membrane bioreactor used in microgravity environment and simulated microgravity environment |
| CN101372664B (en) * | 2008-05-16 | 2012-01-11 | 北京航空航天大学 | Tissue engineering reactor having tissue cultures tension-compression and rotation functions |
| CN108085254A (en) * | 2018-02-09 | 2018-05-29 | 中国人民解放军第四军医大学 | Tissue engineering product culture apparatus |
| CN109804059A (en) * | 2016-08-03 | 2019-05-24 | 诺斯威克公园医学研究所有限公司 | Bioreactor and the method for handling biomaterial |
| WO2019190874A1 (en) * | 2018-03-29 | 2019-10-03 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| US10478822B1 (en) | 2018-04-13 | 2019-11-19 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10550363B1 (en) | 2018-08-14 | 2020-02-04 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US11268061B2 (en) | 2018-08-14 | 2022-03-08 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US11591592B2 (en) | 2020-04-24 | 2023-02-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells using microcarriers |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| US11965154B2 (en) | 2019-08-20 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
-
1999
- 1999-09-20 CN CN99244872U patent/CN2397122Y/en not_active Expired - Fee Related
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372664B (en) * | 2008-05-16 | 2012-01-11 | 北京航空航天大学 | Tissue engineering reactor having tissue cultures tension-compression and rotation functions |
| CN102199537B (en) * | 2011-04-13 | 2013-06-19 | 北京理工大学 | Membrane bioreactor used in microgravity environment and simulated microgravity environment |
| CN102199537A (en) * | 2011-04-13 | 2011-09-28 | 北京理工大学 | Membrane bioreactor used in microgravity environment and simulated microgravity environment |
| CN109804059A (en) * | 2016-08-03 | 2019-05-24 | 诺斯威克公园医学研究所有限公司 | Bioreactor and the method for handling biomaterial |
| CN108085254A (en) * | 2018-02-09 | 2018-05-29 | 中国人民解放军第四军医大学 | Tissue engineering product culture apparatus |
| WO2019190874A1 (en) * | 2018-03-29 | 2019-10-03 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| US10435662B1 (en) | 2018-03-29 | 2019-10-08 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| US10443031B1 (en) | 2018-03-29 | 2019-10-15 | Inscripta, Inc. | Methods for controlling the growth of prokaryotic and eukaryotic cells |
| CN112204131A (en) * | 2018-03-29 | 2021-01-08 | 因思科瑞普特公司 | Automated control of cell growth rate for induction and transformation |
| US10478822B1 (en) | 2018-04-13 | 2019-11-19 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US10550363B1 (en) | 2018-08-14 | 2020-02-04 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11268061B2 (en) | 2018-08-14 | 2022-03-08 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US11078458B2 (en) | 2019-06-21 | 2021-08-03 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US11066675B2 (en) | 2019-06-25 | 2021-07-20 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US11965154B2 (en) | 2019-08-20 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| US11591592B2 (en) | 2020-04-24 | 2023-02-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells using microcarriers |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN2397122Y (en) | Rotary tube cell/tissue culture device | |
| EP0086539B1 (en) | Device for culturing micro-organisms | |
| JP2001502526A (en) | Apparatus and method for culturing cells / tissue | |
| CN101914438B (en) | Low-intensity pulse ultrasound-assisted rotary tubular cell/tissue culture system | |
| CN103305417A (en) | High-yield reactor for protein production, and production method and application thereof | |
| US3769176A (en) | Apparatus and method for microbial fermentation in a zero gravity environment | |
| CN102321537B (en) | Bubbling bioreactor used for plant tissue culture | |
| CN206545023U (en) | Solid microbe production equipment and microbial manure production system | |
| Saxton et al. | Receptor-mediated endocytosis in plants is energetically possible | |
| US20040241835A1 (en) | Dual axis bioreactor, system and method for growing cell or tissue cultures | |
| CN207193305U (en) | Doughnut exchanger and doughnut switch type culture systems | |
| JP2008502331A (en) | Liquid / gas phase exposure reactor for cell culture | |
| CN111019803B (en) | Integrated device for culturing and separating microalgae and system and method thereof | |
| CN1131302C (en) | Threed-dimensional stress adjustable rotary cell/tissue cultivator and its cultivating method | |
| CN103160433A (en) | Rotary barrel type tissue culture device | |
| CN111363680A (en) | Pulse stirring type bioreactor for secretion, separation and collection of exosome | |
| CN113459508A (en) | Multichannel converging type biological printing nozzle | |
| CN102382764B (en) | Rotary cell culture device | |
| CN206783694U (en) | A kind of device for improving cell inoculation dispersiveness | |
| CN112852594A (en) | Cell culture device for biotechnology research | |
| CN218478758U (en) | Microbial inoculum culture apparatus | |
| CN110591913A (en) | Animal and plant cell large-scale culture equipment | |
| CN218755789U (en) | Three-dimensional rotatory culture apparatus of hypoxemia of adjustable speed | |
| CN220564640U (en) | Reactor-based cell culture system | |
| CN111363679B (en) | Method for stimulating large-scale secretion of exosomes by cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |