CN103160433A - Rotary barrel type tissue culture device - Google Patents
Rotary barrel type tissue culture device Download PDFInfo
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- CN103160433A CN103160433A CN 201110414688 CN201110414688A CN103160433A CN 103160433 A CN103160433 A CN 103160433A CN 201110414688 CN201110414688 CN 201110414688 CN 201110414688 A CN201110414688 A CN 201110414688A CN 103160433 A CN103160433 A CN 103160433A
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- motor
- speed change
- change mechanism
- incubator
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Abstract
The invention discloses a rotary barrel type tissue culture device, comprising an incubator main body, a pedestal, a motor and a speed change mechanism. The incubator main body is supported and fixed on the pedestal by supports; the motor is electrically connected with the speed change mechanism; the motor is fixed on the pedestal and then is connected with the speed change mechanism through a rotating shaft. The device is simple in structure and convenient for operations. An inner rotating barrel and an outer rotating barrel (coaxial) can rotate synchronously or differentially. When the inner rotating barrel and the outer rotating barrel rotate synchronously, a flow stress in the incubator is very low, specifically near to zero, thereby facilitating aggregation and adherence of cells; and when the inner rotating barrel and the outer rotating barrel rotate differentially, the flow stress distribution in the incubator can be regulated to facilitate the induction of cell differentiation and the growth of the cells into required tissues. Two supports are arranged, so that the incubator cannot shake when rotating at a high speed, thereby increasing stability of the device.
Description
Technical field
The invention belongs to the tissue engineering technique field, be specifically related to a kind of three-dimensional cultivation device that rotates the cartridge type tissue.
Background technology
Organizational project is the emerging interdisciplinary field that grows up in the last thirty years, and its final purpose is to produce for recovering, keep or promote the product of human body damaged organ or weave construction and function, such as organization engineering skin, bone, cartilage, tendon etc.At present, the bone due to tissue defect and bone tumor or osteopathy excision, bone tuberculosis and infection etc. is damaged day by day common, and both at home and abroad in treatment, the implants (allosome, autologous tissue, artificial tissue) transplanted etc. of adopting carry out operative treatment more.The implant of transplanting due to needs is cellular after treatment, bubble in inner a large amount of space of loosening is difficult to timely eliminating, therefore be rich in marrow blood that stem cell, somatomedin, thrombocyte etc. are conducive to the implant growth and be difficult for entering into transplant inside, be unfavorable for surviving as early as possible and growing of transplant, be difficult to the surgical state that reaches desirable.Need in surgical procedure like this human tissue engineering or allosome, Self-tissue repair material are built enrichment, damaged as example take bone, collect marrow blood in operation, the implant (as allograph bone, artificial bone) that needs are transplanted is conducive to being rich in stem cell, somatomedin, thrombocyte etc. the marrow blood structure enrichment that implant is grown, process again, can accelerate implant and absorbed by the host after transplanting and grow, accelerate rehabilitation.
Summary of the invention
For the problems referred to above, of the present inventionly provide a kind of structure reasonable, operation, rotation cartridge type tissue culture device easy to use.
The technical solution adopted in the present invention is:
a kind of rotation cartridge type tissue culture device, comprise the incubator main body, base, motor and speed change mechanism, the incubator main body is fixed on base with stent support, motor is electrically connected to speed change mechanism, motor is fixed on base, be connected with speed change mechanism by rotating shaft again, also comprise a motor, be fixed on another support, have on a hollow out garden cylinder at the center of cell culture chamber and fix the compound inner core that a gas/liquid exchange membrane consists of, the inner core two ends are fixed on support by rotating shaft, wherein an end rotating shaft is connected with speed change mechanism, speed change mechanism is electrically connected to motor, the end plate that the inner core end exposes the incubator main body is connected with isolation mechanism, isolation mechanism is provided with air inlet, the air outlet, one sealing-ring is arranged between air inlet and air outlet, 3 hollow Piao bolts are arranged on another end plate of incubator main body, press solidly a movable valve under empty Piao bolt, liquid pipe and the nutrient solution of the infusion pump in liquid-supplying system deposited busy device and is connected, after first passing deoscillator by porous tube conduit again, enter cell culture chamber to terminal, the output channel of cell culture chamber is connected in the nutrient solution dialyzer mutually, communicate with nutrient solution withdrawer and metabolite collector respectively, air inlet of air chamber in airing system and source of the gas are by the voltage stabilizing on tracheae, and relief valve connects, the air outlet of air chamber is connected on the residual air collector by vapor pipe.
With respect to prior art, the invention has the advantages that:
1) the inside and outside cylinder of culturing room both can synchronously rotate, and can differentially rotate again; The synchronous rotation makes in culturing room shearing force very low, is beneficial to cell aggregation, adhesion; Differential rotation can regulate and control stress of fluidity in culturing room, with Cell differentiation inducing activity;
2) the rotating shaft double support supports the incubator main body, and the shake in the time of can avoiding speed of rotation higher in order to avoid therefore affect the cell tissue dimensional culture, has improved rotational stabilization.
3) this incubator is simple in structure, and is easy to operate, and whole culturing process is visual.
Description of drawings:
Fig. 1 is the structural representation of apparatus of the present invention.
Embodiment:
Below in conjunction with accompanying drawing, the present invention is further explained explanation.
As shown in Figure 1, a kind of rotation cartridge type tissue culture device comprises: tissue culture room 1, its inwall are gas/liquid exchange membrane-hollow out support composite structures 2; Described gas/liquid exchange membrane-hollow out support composite structure 2 links with inner core rotating shaft 4; Air chamber 3 is the hollow rotating inner core; Inner core rotating shaft 4 is connected with inner core speed change mechanism 6; Urceolus rotary speed shifting mechanism 5 is connected with motor 7; Inner cylinder rotating speed change mechanism 6 is connected with motor 8; Motor 7,8 is commercially available torque motor; Inlet mouth 9 is connected with gas circuit with air outlet 10; Stainless steel stent 11 is used for supporting the incubator main body; Stainless steel stent 11 is fixed on stainless steel base 12; Isolation mechanism 13 does not rotate inlet mouth 9 and air outlet 10 with axle, and import and outlet are kept apart; Bright nutrient solution storage 18; Flow, the infusion pump 19 that pressure is adjustable; The porous tube conduit 20 of nutrient solution input use enters culturing room to culturing room terminal through deoscillator.Deoscillator is in order to regulate culturing room's internal pressure nutrient solution dialyzer 22; Nutrient solution withdrawer 23; Metabolite collector 24: infusion pump wherein: 19 liquid pipe is connected with nutrient solution memory 18, after first passing deoscillator 21 by porous tube conduit 20 again, enter cell culture chamber to culturing room's terminal, the output channel of cell culture chamber is connected and trains liquid dialyzer 22, communicates with nutrient solution withdrawer 23 and metabolite collector 24 respectively; Wherein in air feed-exhaust system: source of the gas 25 use tracheaes are connected with pressure-stabilizing pressure regulation valve, and air pipe outlet is connected to incubator air chamber 7; Among pressure-stabilizing pressure regulation valve 26 is installed the carrier gas road, inlet pipe 27 is connected with incubator inlet mouth 9; Vapor pipe 28 is connected with incubator air outlet 10; In residual air collector 29, air inlet of air chamber is connected by the pressure-stabilizing pressure regulation valve 26 on tracheae with source of the gas 25; The air chamber air outlet connects on She's gas collector 29 by vapor pipe 28.
The inside and outside rotor of this device (coaxial) can synchronous rotary, also can differentially rotate.During synchronous rotary, in incubator, stress of fluidity is very low, goes to zero, and is conducive to cell aggregation, adhesion; During differential rotation, can regulate and control stress of fluidity distribution in culturing room, be beneficial to Cell differentiation inducing activity, grow up to needed tissue.Be provided with two supports, incubator can not shaken when high-speed rotation, thereby has improved the stability of instrument.
Claims (1)
1. one kind is rotated the cartridge type tissue culture device, comprise the incubator main body, base, motor and speed change mechanism, the incubator main body is fixed on base with stent support, motor is electrically connected to speed change mechanism, motor is fixed on base, be connected with speed change mechanism by rotating shaft again, it is characterized in that: comprise that also a motor is fixed on another support, have on a hollow out garden cylinder at the center of cell culture chamber and fix the compound inner core that a gas/liquid exchange membrane consists of, the inner core two ends are fixed on support by rotating shaft, wherein an end rotating shaft is connected with speed change mechanism, speed change mechanism is electrically connected to motor, the end plate that the inner core end exposes the incubator main body is connected with isolation mechanism, isolation mechanism is provided with air inlet, the air outlet, one sealing-ring is arranged between air inlet and air outlet, 3 hollow Piao bolts are arranged on another end plate of incubator main body, press solidly a movable valve under empty Piao bolt, liquid pipe and the nutrient solution of the infusion pump in liquid-supplying system deposited busy device and is connected, after first passing deoscillator by porous tube conduit again, enter cell culture chamber to terminal, the output channel of cell culture chamber is connected in the nutrient solution dialyzer mutually, communicate with nutrient solution withdrawer and metabolite collector respectively, air inlet of air chamber in airing system and source of the gas are by the voltage stabilizing on tracheae, and relief valve connects, the air outlet of air chamber is connected on the residual air collector by vapor pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110414688 CN103160433A (en) | 2011-12-13 | 2011-12-13 | Rotary barrel type tissue culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110414688 CN103160433A (en) | 2011-12-13 | 2011-12-13 | Rotary barrel type tissue culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103160433A true CN103160433A (en) | 2013-06-19 |
Family
ID=48583969
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110414688 Withdrawn CN103160433A (en) | 2011-12-13 | 2011-12-13 | Rotary barrel type tissue culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103160433A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774750A (en) * | 2015-04-24 | 2015-07-15 | 亿利资源集团有限公司 | Photoreactor for culturing microalgae |
| CN114250147A (en) * | 2021-12-29 | 2022-03-29 | 上海日泰医药设备工程有限公司 | Biological reaction device |
| CN114276979A (en) * | 2021-12-29 | 2022-04-05 | 上海日泰医药设备工程有限公司 | Animal cell culture method |
-
2011
- 2011-12-13 CN CN 201110414688 patent/CN103160433A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774750A (en) * | 2015-04-24 | 2015-07-15 | 亿利资源集团有限公司 | Photoreactor for culturing microalgae |
| CN114250147A (en) * | 2021-12-29 | 2022-03-29 | 上海日泰医药设备工程有限公司 | Biological reaction device |
| CN114276979A (en) * | 2021-12-29 | 2022-04-05 | 上海日泰医药设备工程有限公司 | Animal cell culture method |
| CN114276979B (en) * | 2021-12-29 | 2024-05-14 | 上海日泰医药设备工程有限公司 | Animal cell culture method |
| CN114250147B (en) * | 2021-12-29 | 2024-05-14 | 上海日泰医药设备工程有限公司 | Biological reaction device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C04 | Withdrawal of patent application after publication (patent law 2001) | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20130619 |