CN209854174U

CN209854174U - Cell culture device for biotechnology

Info

Publication number: CN209854174U
Application number: CN201821864989.0U
Authority: CN
Inventors: 林博杰; 严玉勇; 黄丹; 邱军
Original assignee: First Affiliated Hospital of Guangxi Medical University
Current assignee: First Affiliated Hospital of Guangxi Medical University
Priority date: 2018-11-13
Filing date: 2018-11-13
Publication date: 2019-12-27
Anticipated expiration: 2028-11-13

Abstract

The utility model discloses a cell culture device for biotechnology, the inside swing joint of through-hole has the straw, the top of straw link up in sampling device's bottom, there is the oxygen hose top one side of incubator, bottom one side of incubator is equipped with U type blast pipe, one side pin joint of incubator has the case lid, one side of case lid and the opposite side of incubator are equipped with the hasp lock of mutual matching, one side of case lid is equipped with the thermometer, inside one side of incubator is equipped with temperature controller, the inside top of incubator is equipped with the light. The utility model discloses place the cell in the culture dish, then put into two sets of incubators, can set up temperature, illumination and oxygen concentration in two sets of incubators respectively, cultivate the cell, the dispersion degree of the contrast of being convenient for cell under different conditions.

Description

Cell culture device for biotechnology

Technical Field

The utility model relates to a cell culture technical field specifically is a cell culture device for biotechnology.

Background

Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. A large number of cells or their metabolites are obtained by cell culture. Since biological products are all derived from cells, it can be said that cell culture technology is the most core and basic technology in biotechnology.

However, the currently commercialized cell culture apparatus, whether it is a relatively small plate, dish, or bottle, or a relatively large spinner flask or cell factory, is an open culture, and it is inconvenient to sample, observe, and compare cells during the cell culture process. To this end, we propose a cell culture device for biotechnology.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell culture device for biotechnology places the cell in the culture dish, then puts into two sets of incubators, can set up temperature, illumination and oxygen concentration in two sets of incubators respectively, cultivates the cell, at the cell culture in-process, need not take out the culture dish, through sampling device, just can directly take a sample to the contrast that provides the cell of not being convenient for in solving above-mentioned background art and the problem of the sample of not being convenient for.

In order to achieve the above object, the utility model provides a following technical scheme: a cell culture device for biotechnology comprises an incubator, wherein a first fixed block with threads is fixedly connected to the bottom of the inner side of the incubator, a threaded sleeve is connected to the outer side of the first fixed block through threads, a second fixed block is connected to the upper end of the threaded sleeve through threads, a sucker is fixedly connected to the top end of the second fixed block, a culture dish is movably connected to the top of the sucker, a through hole is formed in the top of the incubator, a suction pipe is movably connected to the inside of the through hole, the top of the suction pipe penetrates through the bottom of a sampling device, an oxygen pipe penetrates through one side of the top of the incubator, a U-shaped exhaust pipe is arranged on one side of the bottom of the incubator, a case cover is connected to one side of the incubator through a pin, mutually matched hasp locks are arranged on one side of the case cover and the other side of the incubator, a thermometer is arranged on one side of the case, the inside top of incubator is equipped with the light.

Preferably, the sampling device comprises a piston cylinder, the piston is movably connected inside the plug cylinder, a linkage rod is fixedly connected to the top of the piston, a holding part is fixedly connected to the top end of the linkage rod, and the lower end of one side of the plug cylinder is communicated with a liquid outlet pipe.

Preferably, the incubator is provided with two sets of, and the one end of the U type blast pipe of two sets of incubator bottoms all is linked together with the top of blast pipe, and all is equipped with the check valve on the U type blast pipe.

Preferably, the position of the through hole and the center of the culture dish are arranged on the same horizontal plane.

Preferably, a valve is arranged on the oxygen pipe.

Preferably, a sealing ring is arranged inside the through hole.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model places the cells in the culture dish, then places the cells in two groups of culture boxes, and can respectively set the temperature, illumination and oxygen concentration in the two groups of culture boxes to culture the cells, thereby being convenient for comparing the dispersion degree of the cells under different conditions;

2. the utility model discloses a sampling device can be at cell culture's in-process, need not take out the culture dish, takes a sample to the cell between just can, and the straw is inserted in the culture dish, through pulling the piston from top to bottom, just can take out the cell in the culture dish, has avoided taking out the culture dish after, takes a sample again, prevents that the cell from receiving the change of temperature and light and producing the phenomenon of cultivateing the failure.

3. The utility model discloses a sucking disc can be with the firm fixing of culture dish, the height of culture dish is convenient for adjust by the thread bush for the straw can insert in the culture dish, is convenient for take a sample

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of area A in FIG. 1;

fig. 3 is a side view of the present invention.

In the figure: the device comprises a culture box 1, a first fixing block 2, a thread bush 3, a second fixing block 4, a sucker 5, a culture dish 6, a through hole 7, a suction pipe 8, a piston cylinder 9, a piston 10, a linkage rod 11, a holding part 12, a liquid outlet pipe 13, an oxygen pipe 14, a 15U-shaped exhaust pipe, an exhaust pipe 16, a check valve 17, a valve 18, a lighting lamp 19, a temperature controller 20, a snap lock 21, a box cover 22, a thermometer 23, a sealing ring 25 and a sampling device 26.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 3, the present invention provides a technical solution: a cell culture device for biotechnology comprises an incubator 1, wherein a first fixed block 2 with threads is fixedly connected to the bottom of the inner side of the incubator 1, a threaded sleeve 3 is connected to the outer side of the first fixed block 2 in a threaded manner, a second fixed block 4 is connected to the upper end of the threaded sleeve 3 in a threaded manner, a sucker 5 is fixedly connected to the top end of the second fixed block 4, a culture dish 6 is movably connected to the top of the sucker 5, a through hole 7 is formed in the top of the incubator 1, a sucker 8 is movably connected to the inner portion of the through hole 7, the top of the sucker 8 is communicated with the bottom of a sampling device 26, an oxygen pipe 14 is communicated with one side of the top of the incubator 1, a U-shaped exhaust pipe 15 is arranged on one side of the bottom of the incubator 1, a case cover 22 is in pin joint with one side of the incubator 1, and a hasp lock 21 matched with each other is arranged on, a thermometer 23 is arranged on one side of the case cover 22, a temperature controller 20 is arranged on one side of the interior of the incubator 1, the temperature controller 20 is used for controlling the temperature of the interior of the incubator 1, and an illuminating lamp 19 is arranged at the top end of the interior of the incubator 1, so that the illuminating lamp 19 can provide illumination for cells according to needs.

Specifically, sampling device 26 includes a piston cylinder 9, the inside swing joint who fills in a section of thick bamboo 9 has piston 10, the top fixedly connected with gangbar 11 of piston 10, the top fixedly connected with portion of gripping 12 of gangbar 11, one side lower extreme intercommunication that fills in a section of thick bamboo 9 has drain pipe 13, and when needs sample, holding portion of gripping 12 and pulling from top to bottom, drive piston 10 up-and-down motion draws out the cell from culture dish 6, and the convenient sample to the cell need not take out culture dish 6.

Specifically, incubator 1 is provided with two sets ofly, and the one end of the U type blast pipe 15 of two sets of incubators 1 bottoms all is linked together with the top of blast pipe 16, and all be equipped with check valve 17 on the U type blast pipe 15, cell culture's in-process, waste gas can discharge out, waste gas can be under the extrusion of oxygen, discharge in this U type blast pipe 15, this check valve 17 is favorable to preventing the inside of waste gas reflux incubator 1, this two sets of incubators 1's structure is the same setting, but temperature controller 20, light 19 and oxygen hose 14 are mutual independent setting, can carry out the independent control as required.

In particular, the position of the through hole 7 and the centre of the culture dish 6 are arranged at the same level, which facilitates the insertion of the suction tube 8 into the culture dish 6.

Specifically, a valve 18 is arranged on the oxygen pipe 14, and the valve 18 is manually adjusted, so that the oxygen content in the two groups of culture boxes 1 can be respectively adjusted according to needs.

Specifically, the inside of the through hole 7 is provided with a sealing ring 25, and the sealing ring 25 helps to improve the sealing performance inside the incubator 1, so that air does not enter the inside of the incubator 1.

The working principle is as follows: when the device is used, cells are placed in the culture dish 6, then the culture dish 6 is adsorbed on the sucker 5, the second fixing block 4 is driven to adjust up and down by rotating the threaded sleeve 3, the culture dish 6 is driven to move upwards, the suction pipe 8 is inserted into the culture dish 6 and can be carried out as required, the temperature and the light of the culture box 1 are adjusted by the temperature controller 20 and the illuminating lamp 19, oxygen is introduced into the culture box 1 through the oxygen pipe 14, the oxygen can discharge waste gas released by the cells from the U-shaped exhaust pipe 15, when the cells need to be sampled, the holding part 12 is pulled up and down to drive the piston 10 to move up and down, the cells are sucked out of the culture dish 6, the phenomenon that the cells are failed to be cultured due to the change of temperature and light is prevented, the success rate of cell culture is improved, different culture conditions are manufactured by holding the two groups of culture boxes 1, and the cells are cultured, facilitating comparison to cells cultured under which conditions.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell culture device for biotechnology, includes incubator (1), its characterized in that: the utility model discloses a novel incubator, including incubator (1), the inboard bottom fixedly connected with of incubator (1) has screwed first fixed block (2), the outside threaded connection of first fixed block (2) has thread bush (3), the upper end threaded connection of thread bush (3) has second fixed block (4), the top fixedly connected with sucking disc (5) of second fixed block (4), the top swing joint of sucking disc (5) has culture dish (6), the top of incubator (1) is equipped with through-hole (7), the inside swing joint of through-hole (7) has straw (8), the top of straw (8) link up in the bottom of sampling device (26), it has oxygen pipe (14) to link up top one side of incubator (1), bottom one side of incubator (1) is equipped with U type blast pipe (15), one side pin joint of incubator (1) has case lid (22), one side of case lid (22) and the opposite side of incubator (1) are equipped with hasp lock (21) that matches each other, one side of case lid (22) is equipped with thermometer (23), inside one side of incubator (1) is equipped with temperature controller (20), the inside top of incubator (1) is equipped with light (19).

2. A biotechnological cell culture device according to claim 1 characterised by: sampling device (26) include piston cylinder (9), the inside swing joint of stopper cylinder (9) has piston (10), the top fixedly connected with gangbar (11) of piston (10), the top fixedly connected with portion of gripping (12) of gangbar (11), the intercommunication of one side lower extreme of stopper cylinder (9) has drain pipe (13).

3. A biotechnological cell culture device according to claim 1 characterised by: incubator (1) is provided with two sets ofly, and the one end of the U type blast pipe (15) of two sets of incubators (1) bottom all is linked together with the top of blast pipe (16), and all is equipped with check valve (17) on U type blast pipe (15).

4. A biotechnological cell culture device according to claim 1 characterised by: the position of the through hole (7) and the center of the culture dish (6) are arranged on the same horizontal plane.

5. A biotechnological cell culture device according to claim 1 characterised by: a valve (18) is arranged on the oxygen pipe (14).

6. A biotechnological cell culture device according to claim 1 characterised by: and a sealing ring (25) is arranged in the through hole (7).