CN112708560B

CN112708560B - High-efficient formula biological cell culture ware

Info

Publication number: CN112708560B
Application number: CN202110113936.5A
Authority: CN
Inventors: 韩霜; 张可慧; 张庆琛; 石贤萌; 白军令
Original assignee: Shangqiu Normal University
Current assignee: Shangqiu Normal University
Priority date: 2021-01-27
Filing date: 2021-01-27
Publication date: 2022-12-06
Anticipated expiration: 2041-01-27
Also published as: CN112708560A

Abstract

The invention discloses a high-efficiency biological cell incubator, which comprises a box body, wherein a box door is arranged on the outer wall of the box body, a water tank is fixedly connected to the inner side wall of the box body, an electric heating wire is fixedly connected to the inner wall of the bottom of the water tank, a water inlet pipe is fixedly connected to the side wall of the box body, a water valve is slidably connected to the side wall of the water inlet pipe, two opposite sliding grooves are formed in the inner side wall of the box body, two buffer springs are fixedly connected to the bottoms of the inner sides of the two sliding grooves, and a placing table is fixedly connected to the upper surfaces of the buffer springs.

Description

High-efficient formula biological cell culture ware

Technical Field

The invention relates to the technical field of biological culture, in particular to a high-efficiency biological cell culture device.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, suitable temperature, pH value, certain nutritional conditions, etc.) in vitro to make it live, grow, reproduce and maintain main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology, and cell culture is an essential process for both the whole bioengineering technology and one of the techniques, and cell culture technology is an important and common technology in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction of cells, anabolism of cells, growth and proliferation of cells and the like can be researched through the cell culture.

The existing cell culture device is generally used for static culture during culture, partial fungi and partial plant cells are in the static culture process, a culture medium on the bottom layer cannot be in sufficient contact with oxygen, so that the supply of dissolved oxygen is reduced, the culture efficiency is influenced, and partial cell culture devices are in shaking culture during culture.

Disclosure of Invention

The invention aims to solve the defects that the stratum of a culture medium cannot be fully contacted with oxygen in the static culture process of some cells in the prior art, so that the supply of dissolved oxygen is reduced, and the culture efficiency is influenced, and provides a high-efficiency biological cell culture device.

In order to achieve the purpose, the invention adopts the following technical scheme:

the utility model provides a high-efficient formula biological cell culture ware, includes the box, be provided with the chamber door on the outer wall of box, the inside wall fixedly connected with water tank of box, the bottom inner wall fixedly connected with heating wire of water tank, the lateral wall fixedly connected with inlet tube of box, the lateral wall sliding connection of inlet tube has the water valve, two relative spouts, two are seted up to the inside wall of box two buffer springs of inboard bottom fixedly connected with of spout, buffer spring's last fixed surface is connected with places the platform, two last fixed surface fixedly connected with two limiting plates of place the platform, two sets of spring wires of the relative one side fixedly connected with of limiting plate, two sets of two fixed plates of the relative one side fixedly connected with of spring wire, the last fixed surface sliding connection who places the platform has the culture dish, the last fixed surface fixedly connected with exhaust mechanism who places the platform, the lateral wall fixedly connected with blast pipe of box, the blast pipe is the L shape, the other end outer wall of blast pipe and the inside sliding connection who places the platform, the one of the one end fixedly connected with fixed block, one of spout the inside fixedly connected with spring rod, the last fixed surface fixedly connected with mounting panel of spring rod, the intake pipe fixedly connected with of mounting panel, the intake pipe is connected with mechanism.

Preferably, the exhaust mechanism comprises an annular plate fixedly connected to the upper surface of the placing table, the upper surface of the annular plate is rotatably connected with two sliding plates, the side walls of the two sliding plates are fixedly connected with two spring ropes, and the other ends of the two spring ropes are fixedly connected with the side wall of the annular plate.

Preferably, the antibiotic mechanism includes the filtration cotton of fixed connection at the intake pipe lateral wall, the inside wall fixedly connected with ultraviolet lamp of intake pipe, ultraviolet lamp slope sets up, two mirrors of inside wall fixedly connected with of intake pipe, the ultraviolet ray that ultraviolet lamp jetted out is located between two mirrors.

Preferably, a through hole is formed in the annular plate, and the inner side wall of the through hole is in sliding connection with the outer wall of one end of the exhaust pipe.

Preferably, the bottom of the fixing plate is connected with the upper surface of the placing table in a sliding mode.

Preferably, one end of the fixing block, which is far away from the placing table, is in contact with the side wall of the connecting plate.

Preferably, the mounting plate has a width greater than a width of the connecting plate.

Preferably, the one end that the mounting panel was kept away from to the connecting plate extends to the inside of intake pipe, the one end that the mounting panel was kept away from to the connecting plate contacts with the lateral wall of intake pipe.

Compared with the prior art, the invention has the beneficial effects that:

1. open the valve, make water flow into the water tank through the inlet tube in, then start the heating wire, the inside temperature of box will rise, the inside pressure of box can increase when the temperature rises, inside pressure increase can make and place platform compress buffer spring downwards, make buffer spring move down, place the platform when moving down simultaneously, the blast pipe can support the slide, make the slide rebound, open the check valve, the inside unnecessary air of box will pass through the blast pipe discharge this moment, along with the decline of placing the platform, fix and also can descend thereupon placing the fixed block of platform one end, when the fixed block descends, can contact the mounting panel, make the mounting panel move down, the mounting panel moves down can drive the spring beam downward compression, make the connecting plate move down, the sterile air in the intake pipe can enter into the inside of box this moment, make the inside circulation of air of box, when the inside sterile air of intake pipe comes in, can reduce the inside temperature of box.

To sum up, through mutually supporting between heating wire, blast pipe, the intake pipe, realized the inside temperature rise of box, solved the inside cell of incubator when cultivateing the temperature and hang down to influence the reproduction problem of cell, the inside circulation of air of box has avoided the inside high temperature of box to lead to the cell death simultaneously, has realized that the box is inside can keep homothermal state, makes the cell reproduction in aseptic environment.

2. Through the inside pressure increase of box, make and place the platform downstream, through buffer spring, the blast pipe, the slide, the spring rope, the fixed block, the mounting panel, the spring beam, mutually support between the connecting plate, can make and place the platform at the uniform velocity and reciprocate in the box, make the inside culture medium of culture dish can vibrate, the culture medium and the oxygen of bottom fully contact, thereby improve the supply volume of dissolved oxygen, simultaneously through the limiting plate, the spring silk, cooperation between the fixed plate, it is fixed to carry out the centre gripping to the culture dish in the box, it can appear sliding or even toppling over to have avoided the oscillation in-process culture dish, and the sample that leads to in the culture dish spills the condition in the box that falls.

3. When outside air comes in through the intake pipe, can pass through lens, ultraviolet lamp, filtration cotton in proper order, when the air comes in, the ultraviolet ray that the ultraviolet lamp shines out can shine on the lens, forms folding ray, effectually disinfects to the air, and the air after disinfecting simultaneously is filtered the back through the filtration cotton and is reentrant in the box, avoids leading to the cell in the culture dish to be polluted and die because of harmful gas such as virus, bacterium in the air.

Drawings

FIG. 1 is a schematic structural diagram of a high-efficiency biological cell culture apparatus according to the present invention;

FIG. 2 is a front sectional view of a high efficiency biological cell culture apparatus according to the present invention;

FIG. 3 is an enlarged close-up view of the position B in the high-efficiency biological cell culture apparatus according to the present invention;

FIG. 4 is an enlarged view of the high efficiency biological cell culture apparatus of the present invention at B;

FIG. 5 is an enlarged view of the position A in the high efficiency biological cell culture apparatus according to the present invention;

FIG. 6 is an enlarged view of the position C of the high efficiency biological cell culture apparatus according to the present invention.

In the figure: the device comprises a box body 1, a limiting plate 2, a culture dish 3, a buffer spring 4, an exhaust pipe 5, a placing table 6, an air inlet pipe 7, air filter cotton 8, an ultraviolet lamp 9, a mirror 10, ultraviolet rays 11, a ring-shaped plate 12, a sliding plate 13, a spring rope 14, a water tank 15, an electric heating wire 16, a water inlet pipe 17, a box door 18, a spring rod 19, a mounting plate 20, a connecting plate 21, a fixing block 22, a water valve 23, a check valve 24, a spring wire 25 and a fixing plate 26.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-6, a high-efficiency biological cell incubator comprises a box body 1, a box door 18 is arranged on the outer wall of the box body 1, a water tank 15 is fixedly connected to the inner side wall of the box body 1, an electric heating wire 16 is fixedly connected to the inner wall of the bottom of the water tank 15, the electric heating wire 16 heats water in the water tank 15, so that a certain humidity can be maintained in the box body 1, and reproduction of cells is facilitated, a valve 23 is opened, so that water flows into the water tank 15 through a water inlet pipe 17, then the electric heating wire 16 is started, the temperature in the box body 1 rises, the pressure in the box body 1 increases while the temperature rises, the placing table 6 compresses a buffer spring 4 downwards to enable the buffer spring 4 to move downwards, meanwhile, when the placing table 6 moves downwards, an exhaust pipe 5 abuts against a sliding plate 13, the sliding plate 13 moves upwards, a one-way valve 24 is opened, redundant air in the box body 1 can be exhausted through the exhaust pipe 5, as the placing table 6 descends, when the fixing block 22 descends, the mounting plate 20 contacts with the mounting plate 20, the mounting plate 20 moves downwards, the mounting plate 20 drives a germ-free air rod 7 to move downwards, the air in the box body 1 can not enter, and the box body 1, and when the air in the box body 1, the sterile air in the box body 1 can not enter, and the sterile air, so that the sterile air in the sterile box body 1, and the sterile air is prevented from entering the sterile air, and the sterile air in the sterile box body 1, and the sterile air in the sterile box body 7, and the sterile air is prevented from being compressed air in the sterile air, and the sterile air in the sterile box body 1, and the sterile box body 7.

The side wall of the box body 1 is fixedly connected with a water inlet pipe 17, the side wall of the water inlet pipe 17 is connected with a water valve 23 in a sliding manner, the inner side wall of the box body 1 is provided with two opposite sliding chutes, the bottoms of the inner sides of the two sliding chutes are fixedly connected with two buffer springs 4, the upper surface of the buffer springs 4 is fixedly connected with a placing table 6, the upper surface of the placing table 6 is fixedly connected with two limiting plates 2, one opposite sides of the two limiting plates 2 are fixedly connected with two groups of spring wires 25, one opposite sides of the two groups of spring wires 25 are fixedly connected with two fixing plates 26, the bottoms of the fixing plates 26 are connected with the upper surface of the placing table 6 in a sliding manner, the upper surface of the placing table 6 is connected with a culture dish 3 in a sliding manner, the upper surface of the placing table 6 is fixedly connected with an exhaust mechanism, the exhaust mechanism comprises a ring-shaped plate 12 fixedly connected with the upper surface of the placing table 6, and the upper surface of the ring-shaped plate 12 is rotatably connected with two sliding plates 13, the side walls of the two sliding plates 13 are fixedly connected with two spring ropes 14, the other ends of the two spring ropes 14 are fixedly connected with the side wall of the annular plate 12, the side wall of the box body 1 is fixedly connected with an exhaust pipe 5, a through hole is arranged inside the annular plate 12, the inner side wall of the through hole is in sliding connection with the outer wall of one end of the exhaust pipe 5, the exhaust pipe 5 is L-shaped, the outer wall of the other end of the exhaust pipe 5 is in sliding connection with the inner side wall of the placing table 6, one end of the placing table 6 is fixedly connected with a fixed block 22, the inner side wall of the bottom of one sliding chute is fixedly connected with a spring rod 19, the upper surface of the spring rod 19 is fixedly connected with a mounting plate 20, the upper surface of the mounting plate 20 is fixedly connected with a connecting plate 21, the width of the mounting plate 20 is larger than that of the connecting plate 21, one end of the connecting plate 21, which is far away from the mounting plate 20, extends to the inside of the air inlet pipe 7, and one end of the connecting plate 21, which is far away from the mounting plate 20, is in contact with the side wall of the air inlet pipe 7, the lateral wall fixedly connected with intake pipe 7 of box 1, increase through the inside pressure of box 1, make and place platform 6 downstream, through buffer spring 4, blast pipe 5, slide 13, spring rope 14, fixed block 22, mounting panel 20, spring beam 19, mutually support between the connecting plate 21, can make and place platform 6 reciprocate in box 1, make the inside culture medium of culture dish 3 shake, the culture medium and the oxygen of bottom fully contact, thereby improve the supply of dissolved oxygen, the culture efficiency of cell has been improved, simultaneously through limiting plate 2, spring wire 25, cooperation between the fixed plate 26, it is fixed to carry out the centre gripping to culture dish 3 in the box 1, it can appear slipping even toppling over to have avoided oscillation process culture dish 3, and lead to the sample in the culture dish 3 to spill the condition in the box 1.

The inside fixedly connected with antibacterial mechanism of intake pipe 7, antibacterial mechanism includes the filtration cotton 8 of fixed connection at the 7 lateral walls of intake pipe, the inside wall fixedly connected with ultraviolet lamp 9 of intake pipe 7, the slope of ultraviolet lamp 9 sets up, two mirrors 10 of inside wall fixedly connected with of intake pipe 7, ultraviolet lamp 9 emits ultraviolet ray 11 between two mirrors 10, when outside air comes in through intake pipe 7, can pass through lens 10 in proper order, ultraviolet lamp 9, filtration cotton 8, when the air comes in, ultraviolet lamp 9 shines out ultraviolet ray 11 can shine on lens 10, form folding ray, effectually disinfect to the air, the air after disinfecting simultaneously gets into in box 1 after filtration of filtration cotton 8, avoid containing the virus in the air, harmful gas such as bacterium leads to the cell in the culture dish 3 to be polluted and die.

The functional principle of the invention can be illustrated by the following operation modes:

when the incubator works, the culture dish 3 is placed between the two fixing blocks 26, the valve 23 is opened, water flows into the water tank 15 through the water inlet pipe 17, then the heating wire 16 is started to heat the water inside the water tank 15, certain humidity can be kept inside the box body 1, the temperature inside the box body 1 can rise during heating, the pressure inside the box body 1 can increase during temperature rising, the placing table 6 can compress the buffer spring 4 downwards due to the increase of the internal pressure, the buffer spring 4 can move downwards, meanwhile, when the placing table 6 moves downwards, the exhaust pipe 5 can abut against the sliding plate 13, the sliding plate 13 can move upwards, the one-way valve 24 is opened, redundant air inside the box body 1 can be discharged through the exhaust pipe 5, the fixing block 22 fixed at one end of the placing table 6 can also descend along with the descending of the placing table 6, when the fixing block 22 descends, the mounting plate 20 can be contacted, the mounting plate 20 can move downwards, the mounting plate 20 can drive the spring rod 19 to compress downwards, the air inside the connecting plate 21 can move downwards, at the sterile air inside the air in the box body 1 can enter, the connecting pipe 7, and the sterile air can be prevented from flowing into the box body 1 when the temperature is high.

Through the increase of the pressure inside the box 1, make and place platform 6 and move down, through buffer spring 4, blast pipe 5, slide 13, spring rope 14, fixed block 22, mounting panel 20, spring beam 19, mutually support between the connecting plate 21, can make and place platform 6 and reciprocate in the box 1, make the culture medium inside culture dish 3 shake, the culture medium of bottom fully contacts with oxygen, thereby improve the supply of dissolved oxygen, the culture efficiency of cell has been improved, simultaneously through the limiting plate 2, spring wire 25, cooperation between the fixed plate 26, it is fixed to carry out the centre gripping to culture dish 3 in the box 1, it can appear slipping even toppling over to have avoided oscillation process culture dish 3, and lead to the sample in the culture dish 3 to spill the condition in the box 1.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. The high-efficiency biological cell culture device comprises a box body (1) and is characterized in that a box door (18) is arranged on the outer wall of the box body (1), a water tank (15) is fixedly connected to the inner side wall of the box body (1), heating wires (16) are fixedly connected to the inner bottom wall of the water tank (15), a water inlet pipe (17) is fixedly connected to the side wall of the box body (1), a water valve (23) is slidably connected to the side wall of the water inlet pipe (17), two opposite sliding grooves are formed in the inner side wall of the box body (1), two buffer springs (4) are fixedly connected to the inner bottom of the two sliding grooves, a placing table (6) is fixedly connected to the upper surface of each buffer spring (4), two limiting plates (2) are fixedly connected to the upper surface of the placing table (6), two sets of spring wires (25) are fixedly connected to one side opposite to the two limiting plates (2), two sets of spring wires (25) are fixedly connected to one side opposite to the other fixing plates (26), a culture dish (3) is slidably connected to the upper surface of the placing table (6), an exhaust mechanism is fixedly connected to the side wall of the placing table (6), the box body (1) is fixedly connected to the other end of the exhaust pipe (5), and the exhaust pipe (5) is connected to the outer wall of the exhaust pipe (6) and is connected to the exhaust pipe (5) and is connected to the inner side wall of the exhaust pipe (6), one end of the placing table (6) is fixedly connected with a fixed block (22), the inner side wall of the bottom of one sliding groove is fixedly connected with a spring rod (19), the upper surface of the spring rod (19) is fixedly connected with an installation plate (20), the upper surface of the installation plate (20) is fixedly connected with a connecting plate (21), the side wall of the box body (1) is fixedly connected with an air inlet pipe (7), and the interior of the air inlet pipe (7) is fixedly connected with an antibacterial mechanism; the exhaust mechanism comprises an annular plate (12) fixedly connected to the upper surface of the placing table (6), the upper surface of the annular plate (12) is rotatably connected with two sliding plates (13), the side walls of the two sliding plates (13) are fixedly connected with two spring ropes (14), and the other ends of the two spring ropes (14) are fixedly connected with the side wall of the annular plate (12); fungi-proofing mechanism includes air filter cotton (8) of fixed connection at intake pipe (7) lateral wall, the inside wall fixedly connected with ultraviolet lamp (9) of intake pipe (7), ultraviolet lamp (9) slope sets up, two mirrors (10) of inside wall fixedly connected with of intake pipe (7), ultraviolet ray (11) that ultraviolet lamp (9) jetted out are located between two mirrors (10).

2. The high-efficiency biological cell culture device according to claim 1, wherein the annular plate (12) is provided with a through hole therein, and the inner side wall of the through hole is slidably connected with the outer wall of one end of the exhaust pipe (5).

3. The high-efficiency biological cell culture device according to claim 1, wherein the bottom of the fixing plate (26) is slidably connected with the upper surface of the placing table (6).

4. The high-efficiency biological cell culture device according to claim 1, wherein one end of the fixing block (22) far from the placing table (6) is in contact with the side wall of the connecting plate (21).

5. A high efficiency biological cell culture apparatus as claimed in claim 1, wherein the width of the mounting plate (20) is greater than the width of the connection plate (21).

6. The high-efficiency biological cell culture device according to claim 1, wherein one end of the connecting plate (21) far away from the mounting plate (20) extends to the inside of the air inlet pipe (7), and one end of the connecting plate (21) far away from the mounting plate (20) is in contact with the side wall of the air inlet pipe (7).