CN115747056A

CN115747056A - Tumor cell culture device

Info

Publication number: CN115747056A
Application number: CN202211470750.6A
Authority: CN
Inventors: 李婷; 陈卫; 刘肖洁
Original assignee: North Sichuan Medical College
Current assignee: North Sichuan Medical College
Priority date: 2022-11-23
Filing date: 2022-11-23
Publication date: 2023-03-07

Abstract

The invention discloses a tumor cell culture device, which comprises a box body, wherein a disinfection component is arranged on the box body, a plurality of electric push doors are arranged in the box body, and the box body is divided into a culture cavity and a taking cavity by the electric push doors; the taking cavity is provided with a taking window; a plurality of placing racks are arranged in the culture cavity at intervals up and down, a plurality of culture dishes are detachably connected to the placing racks, and the liquid inlet ends of the culture dishes are connected with elastic top covers in a sliding manner; the culture cavity is provided with a movable liquid feeding mechanism for feeding liquid into a plurality of culture dishes, and the output end of the movable liquid feeding mechanism is provided with a lifting rod for controlling the opening and closing of the elastic top cover; the intracavity of taking is installed and is used for getting to put and transmit the mechanical clamping jaw of culture dish, installs the regulation and control system on the box for the wind pressure of regulation and control intracavity greenhouse, humidity, wind pressure and regulation and control intracavity of taking. The invention can complete liquid feeding in the culture cavity, and can avoid exposing all culture dishes in the air during sampling, thereby reducing bacterial infection and being beneficial to the culture of tumor cells.

Description

Tumor cell culture device

Technical Field

The invention relates to the technical field of cell culture devices, in particular to a tumor cell culture device.

Background

Tumors are one of the most difficult diseases to cure in human, and in order to prevent and treat tumors at an early stage and avoid tumor formation, it is necessary to perform repeated and intensive studies by obtaining a new tumor cell population through cell culture, and to study a pharmaceutical agent and the like capable of targeting tumor cells. When tumor cells are cultured, a plurality of culture dishes are generally placed in an incubator, nutrient solution needs to be added into the culture dishes in the culture process, the culture dishes are taken out for sampling, observation and recording, and the incubator needs to be opened and closed frequently, so that all the culture dishes are exposed in the air frequently, the original environment in the incubator is damaged, the growth of the tumor cells is not facilitated, bacteria are easy to invade in the incubator, the tumor cells in the culture dishes are infected, and the experiment is influenced; in addition, most of the culture dishes on the market are mainly cultured in a cell-adherent way when tumor cells are cultured, but the culture way does not accord with the growth characteristics of the tumor cells. The special culture dish arranged in the culture box is specially designed aiming at the characteristic of the aggregate growth of the tumor cells, accords with the natural growth characteristic of the tumor cells, and is more favorable for tumor research.

Therefore, a tumor cell culture device is provided.

Disclosure of Invention

The present invention aims to provide a tumor cell culture apparatus, which aims to solve or improve at least one of the technical problems.

In order to achieve the purpose, the invention provides the following scheme: the present invention provides a tumor cell culture apparatus comprising:

the sterilization device comprises a box body, wherein a sterilization component is arranged on the box body, a plurality of electric push doors are arranged in the box body, and the box body is divided into a culture cavity and a taking cavity by the electric push doors; the taking cavity is provided with a taking window;

the placing racks are fixedly connected in the culture cavity at intervals up and down, the placing racks are detachably connected with a plurality of culture dishes, liquid inlet ends of the culture dishes are connected with an elastic top cover in a sliding mode, and a plurality of second grooves are formed in the bottoms of the culture dishes;

the movable liquid adding mechanism is arranged on the culture cavity and is used for adding liquid into the culture dishes; the output end of the movable liquid adding mechanism is provided with a lifting rod for controlling the opening and closing of the elastic top cover;

the mechanical clamping jaw is arranged in the taking cavity and used for taking, placing and transferring the culture dish;

the regulation and control system is installed on the box body and used for regulating and controlling greenhouse, humidity, wind pressure and regulation and control in the culture cavity and the wind pressure in the taking cavity.

Preferably, the movable liquid adding mechanism comprises a first lead screw motor fixedly connected to the outer wall of the box body, an output shaft of the first lead screw motor extends into the culture cavity and is provided with a first lead screw, and a first sliding sleeve slidably connected with the bottom of the culture cavity is slidably sleeved on the first lead screw; a support rod is fixedly connected to the first sliding sleeve, and a liquid adding assembly and the lifting rod are fixedly connected to the support rod;

the lifting rod comprises a plurality of supporting rods fixedly connected to the supporting rod, the supporting rods and the placing frames are arranged in a one-to-one correspondence mode, first air cylinders are fixedly connected to the bottoms of the supporting rods, and piston ends of the first air cylinders are detachably connected with the elastic top covers; the liquid feeding assembly is used for feeding liquid into the culture dishes.

Preferably, the liquid adding assembly comprises a liquid adding main pipe fixedly connected to the support rod, a plurality of liquid adding branch pipes are fixedly connected to the liquid adding main pipe, the liquid adding branch pipes and the placing racks are arranged in a one-to-one correspondence manner, a liquid adding head and an electromagnetic valve are fixedly connected to the liquid adding branch pipes, and the liquid adding head is located above the elastic top cover; the utility model discloses a culture apparatus, including culture chamber, liquid feeding house steward, culture chamber and first water pump, the liquid feeding house steward is kept away from the one end rigid coupling that the liquid feeding was in charge of has flexible hose, flexible hose stretches out to it has the liquid reserve tank to cultivate the chamber outer and rigid coupling, the liquid reserve tank rigid coupling is in on the box outer wall, install first water pump in the liquid reserve tank, the last flow valve of installing of flexible hose.

Preferably, the mechanical clamping jaw comprises a second lead screw motor fixedly connected to the outer wall of the box body, an output shaft of the second lead screw motor extends into the taking cavity and is provided with a second lead screw, and a second sliding sleeve slidably connected with the inner wall of the taking cavity is sleeved on the second lead screw in a sliding manner;

install first step motor on the second sliding sleeve, the rigid coupling has the second cylinder on the output shaft of first step motor, the piston end rigid coupling of second cylinder has the third cylinder, the second cylinder with the third cylinder sets up perpendicularly, the piston end rigid coupling of third cylinder has the clamping jaw motor, the clamping jaw that two are relative to setting is installed to the output of clamping jaw motor, two the clamping jaw with the connection can be dismantled to the culture dish.

A plurality of fourth sliding chutes arranged in parallel and a plurality of third sliding chutes arranged opposite to the fourth sliding chutes are formed in the inner side wall of the box body;

the electric sliding door is connected including rotating third lead screw in the third spout, third lead screw one end rigid coupling has third lead screw motor, third lead screw motor rigid coupling is in on the box outer wall, on the third lead screw slide the cover be equipped with third spout sliding connection's third slider, the rigid coupling has the slide on the third slider, adjacent two sliding contact between the slide, the slide is kept away from the one end of third slider with fourth spout sliding connection.

Preferably, the placing rack is provided with a plurality of first air holes, and one side, far away from the clamping jaw, of the top surface of the placing rack is fixedly connected with a limit stop;

the culture dish comprises a clamping shell, and the clamping shell is placed on the placing frame and is clamped with the limit stop; the clamping jaw is detachably connected with the clamping shell; the bottom of the clamping shell is provided with a second air hole, the clamping shell is internally clamped with a culture shell, the top of the culture shell is in threaded connection with a culture cover, the culture cover is fixedly connected with a liquid inlet pipe, and the liquid inlet pipe is provided with the elastic top cover; the bottom wall in the culture shell is provided with a plurality of second grooves.

Preferably, the elastic top cover comprises:

the sleeve is fixedly connected to the outer wall of the liquid inlet pipe; two first sliding grooves are formed in the inner wall of the sleeve;

the top cover is connected with the top of the liquid inlet pipe in a sliding manner, and the liquid feeding head is positioned above the top cover;

the sliding rod is fixedly connected with one end of the top cover close to the sleeve, two sliding blocks are fixedly connected to the side wall of the sliding rod, the two sliding blocks are respectively in sliding connection with the two first sliding chutes,

the spring is fixedly connected between the sliding rod and the sleeve;

the groove is formed in one end, far away from the sliding rod, of the top cover; the groove is clamped with the piston end of the first cylinder.

Preferably, the regulatory system comprises:

the temperature adjusting component is arranged on the culture cavity and is used for adjusting the air temperature in the culture cavity;

the pressure regulating assembly is arranged on the culture cavity and the taking cavity and is used for regulating the air pressure in the culture cavity and the taking cavity;

the humidifying component comprises a water storage bin fixedly connected to the outer wall of the box body, a second water pump is fixedly connected in the water storage bin, a water outlet pipe is fixedly connected on the water storage bin, the water outlet pipe extends into the culture cavity and is fixedly connected with a water distribution pipe, and a plurality of nozzles are fixedly connected on the water distribution pipe; the plurality of nozzles are positioned below the temperature adjusting assembly and above the plurality of placing frames; a humidity sensor is arranged in the culture cavity;

an air outlet assembly; the air outlet assembly comprises an exhaust pipe fixedly connected to the culture cavity, and one end of the exhaust pipe extends out of the culture cavity and is fixedly connected with a one-way valve.

Preferably, the temperature adjusting component comprises a housing fixedly connected to the top wall of the culture chamber, the housing is positioned above the plurality of nozzles, a plurality of electric heating plates are embedded in the side wall of the housing, and a third air hole is formed in the bottom of the housing; a temperature sensor is arranged in the culture cavity;

the pressure regulating assembly comprises two air pumps fixedly connected to the outer wall of the box body, and the air outlet ends of the two air pumps are respectively communicated with the taking cavity and the inner cavity of the housing; an air filter element is arranged at the air inlet end of the air pump; and the taking cavity and the culture cavity are internally provided with air pressure sensors.

Preferably, the disinfection component comprises a plurality of ultraviolet disinfection lamps fixedly connected to the inner wall of the culture cavity and the inner wall of the taking cavity.

The invention discloses the following technical effects: the culture dish is used for placing the cultured tumor cells, and the second groove is formed in the bottom of the culture dish, so that the tumor cells can conveniently aggregate and grow at the second groove in the culture process, the growth characteristics of the tumor cells are adapted, the natural growth rule of the tumor cells is closer to, the culture of the tumor cells is facilitated, and the subsequent analysis and research on the tumor cells are improved; the regulation and control system enables the culture cavity to keep proper temperature, humidity and pressure to keep normal growth of tumor cells, enables the air pressure of the culture cavity to be larger than that of the taking cavity, and enables the air pressure of the taking cavity to be larger than that of the outside, so that the entering of outside air into the culture cavity is reduced, the risk of bacterial infection is reduced, when nutrient solution needs to be added, the liquid adding mechanism is moved to the position above a culture dish needing liquid adding, the lifting rod is synchronously driven to open the elastic top cover, and then the nutrient solution is added into the culture dish by moving the liquid adding mechanism; liquid can be added in the culture cavity without taking out the culture dish, so that the environment in the culture cavity is not influenced and bacteria are prevented from entering the culture cavity; when one of the culture dishes needs to be taken out, the electric push door is opened, the mechanical clamping jaw grabs the corresponding culture dish and transmits the culture dish to the taking window, the electric push door is closed, the taking window is opened, and the culture dish is taken out for sampling, observing and recording; avoid exposing all culture dishes in the outside air, reduce the influence to the culture intracavity environment simultaneously, do benefit to the normal growth of tumour cell in the culture dish, to a little entering culture chamber, take the outside air of intracavity, the disinfection subassembly carry out germicidal treatment can.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a front sectional view of a tumor cell culture apparatus according to the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1;

FIG. 3 is a top cross-sectional view of the present invention;

FIG. 4 is a partial enlarged view of B in FIG. 3;

FIG. 5 is a schematic view of the mounting of the housing of the present invention;

FIG. 6 is a schematic view of the installation of the flexible top cover of the present invention;

FIG. 7 is a schematic structural view in example 2 of the present invention;

FIG. 8 is a rear view in the embodiment 2 of the present invention;

FIG. 9 is a schematic structural view in example 3 of the present invention;

wherein, 1, a box body; 2. a culture chamber; 3. a cavity is fetched; 4. taking a window; 5. placing a rack; 6. a liquid inlet pipe; 7. a first sliding sleeve; 8. a top cover; 9. a groove; 10. a sleeve; 11. a first chute; 12. a slide bar; 13. a slider; 14. a spring; 15. a liquid feeding main pipe; 16. liquid adding branch pipe; 17. a liquid adding head; 18. a flexible hose; 19. a liquid storage tank; 20. a collection box; 21. a strut; 22. a first cylinder; 23. a first lead screw motor; 24. a first lead screw; 25. a support bar; 26. a second lead screw motor; 27. a second lead screw; 28. a second sliding sleeve; 29. a first stepper motor; 30. a second cylinder; 31. a third cylinder; 32. a jaw motor; 33. a clamping jaw; 34. a fourth chute; 35. a third chute; 36. a slide plate; 37. a third slider; 38. a third lead screw; 39. a third lead screw motor; 40. a first air vent; 41. a limit stop block; 42. a clamping shell; 43. a second air hole; 44. culturing the shell; 45. a culture cover; 46. a buffer net; 47. a housing; 48. an electric hot plate; 49. a third air hole; 50. an air pressure sensor; 51. an exhaust pipe; 52. a one-way valve; 53. a water storage bin; 54. a water outlet pipe; 55. a water outlet valve; 56. a water distribution pipe; 57. a nozzle; 58. an air pump; 59. an ultraviolet disinfection lamp; 60. a humidity sensor; 61. a temperature sensor; 62. an electromagnetic valve; 63. a water tank; 64. cleaning the pipe; 65. a three-way valve; 66. a water outlet pipe; 67. a stopper; 68. a buffer layer; 69. a flow-through hole; 70. a second groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

Example 1

Referring to fig. 1 to 7, the present invention provides a tumor cell culture apparatus comprising:

the disinfection device comprises a box body 1, wherein a disinfection component is arranged on the box body 1, a plurality of electric push doors are arranged in the box body 1, and the electric push doors divide the interior of the box body 1 into a culture cavity 2 and a taking cavity 3; the taking cavity 3 is provided with a taking window 4;

the top of the box body 1 is provided with a box door (not shown in the figure) which is detachably connected, so that the maintenance of the interior of the box body 1 is facilitated; the size of the section of the taking window 4 is larger than that of the culture dish, so that the culture dish can be taken out, and the external air is reduced to the greatest extent to enter the taking cavity 3; the box body 1 is provided with a control system for controlling the regulation and control system and the on-off of each electric device; the culture cavity 2 is communicated with a sealing pipe (not shown in the figure), the sealing pipe is fixedly connected with a carbon dioxide bottle (not shown in the figure), a carbon dioxide concentration sensor (not shown in the figure) is installed in the culture cavity 2, carbon dioxide is introduced into the culture cavity 2 through the carbon dioxide bottle, the carbon dioxide concentration sensor detects the concentration of the carbon dioxide in the culture cavity 2 and transmits detected concentration information to the control system, and when the carbon dioxide in the culture cavity 2 reaches a specified concentration, the control system controls the carbon dioxide bottle to be closed and stops supplying air, so that the culture cavity 2 keeps a proper carbon dioxide concentration, and the tumor cells are cultured;

the culture device comprises a plurality of placing frames 5, wherein the placing frames 5 are fixedly connected in a culture cavity 2 at intervals up and down, a plurality of culture dishes are detachably connected to the placing frames 5, the liquid inlet ends of the culture dishes are connected with an elastic top cover in a sliding mode, and a plurality of second grooves 70 are formed in the bottoms of the culture dishes; the bottom of the culture dish is of an uneven structure by arranging the second grooves 70, the section of each second groove 70 is U-shaped, when tumor cells are cultured, the tumor cells can grow in the second grooves 70 in an aggregating manner, the natural growth rule of the tumor cells is closer to, the existing adherent culture mode of the tumor cells is changed, the culture of the tumor cells is more facilitated, and the subsequent analysis and research on the tumor cells are improved;

the movable liquid adding mechanism is arranged on the culture cavity 2 and is used for adding liquid into the culture dishes; the output end of the movable liquid adding mechanism is provided with a lifting rod for controlling the opening and closing of the elastic top cover;

the mechanical clamping jaw is arranged in the taking cavity 3 and is used for taking, placing and transferring the culture dish;

the regulating system is arranged on the box body 1 and is used for regulating and controlling the greenhouse, the humidity and the air pressure in the culture cavity 2 and the air pressure in the taking cavity 3;

the regulation and control system ensures that the culture cavity 2 is kept at proper temperature, humidity and pressure, thereby being beneficial to the normal growth of tumor cells in the culture dish; the regulation and control system enables the air pressure of the taking cavity 3 to be larger than the external air pressure, and when the taking window 4 is opened, the external air is reduced from entering the taking cavity 3, so that the risk of bacterial infection is reduced; the regulation and control system enables the air pressure of the taking cavity 3 to be smaller than that of the culture cavity 2, when the culture dish is clamped from the culture cavity 2 by the mechanical clamping jaw, the air of the taking cavity 3 is reduced to enter the culture cavity 2, and a small amount of air entering the taking cavity 3 and the culture cavity 2 is disinfected by the disinfection component, so that bacterial infection of cells is prevented;

placing tumor cells to be cultured in a culture dish, disinfecting the culture dish, opening a taking window 4, grabbing the culture dish by a mechanical clamping jaw, closing the taking window 4, opening an electric push door, placing the culture dish on a placing frame 5 by the mechanical clamping jaw, closing the electric push door, and repeating the steps to sequentially place the culture dish;

when nutrient solution needs to be added into the culture dish, the movable liquid adding mechanism moves to the position above the culture dish needing liquid adding, the elastic top cover is driven to open by the lifting rod, and the nutrient solution is added into the culture dish by moving the liquid adding mechanism; liquid can be added in the culture cavity without taking out the culture dish, so that the environment in the culture cavity is not influenced, and bacteria are prevented from entering the culture cavity; when one of the culture dishes needs to be taken out, the electric push door is opened, the mechanical clamping jaw grabs the corresponding culture dish and transfers the culture dish to the taking window 4, the electric push door is closed, the taking window 4 is opened, and the culture dish is taken out for sampling, observing and recording; avoid exposing all culture dishes in the outside air, reduce the influence to culture intracavity environment simultaneously, do benefit to the normal growth of tumour cell in the culture dish.

According to a further optimized scheme, the movable liquid adding mechanism comprises a first lead screw motor 23 fixedly connected to the outer wall of the box body 1, an output shaft of the first lead screw motor 23 extends into the culture chamber 2 and is provided with a first lead screw 24, and a first sliding sleeve 7 in sliding connection with the bottom of the culture chamber 2 is slidably sleeved on the first lead screw 24; a support rod 25 is fixedly connected to the first sliding sleeve 7, and a liquid feeding assembly and a lifting rod are fixedly connected to the support rod 25;

the lifting rod comprises a plurality of supporting rods 21 fixedly connected to the supporting rod 25, the supporting rods 21 are arranged in one-to-one correspondence with the placing frames 5, the bottoms of the supporting rods 21 are fixedly connected with first air cylinders 22, and the piston ends of the first air cylinders 22 are detachably connected with the elastic top cover; the liquid adding assembly is used for adding liquid into the culture dishes;

the liquid adding assembly comprises a liquid adding main pipe 15 fixedly connected on the support rod 25, a plurality of liquid adding branch pipes 16 are fixedly connected on the liquid adding main pipe 15, the plurality of liquid adding branch pipes 16 are arranged in one-to-one correspondence with the plurality of placing frames 5, a liquid adding head 17 and an electromagnetic valve 62 are fixedly connected on the liquid adding branch pipes 16, and the liquid adding head 17 is positioned above the elastic top cover; one end of the main liquid adding pipe 15, which is far away from the branch liquid adding pipe 16, is fixedly connected with a flexible hose 18, the flexible hose 18 extends out of the culture chamber 2 and is fixedly connected with a liquid storage tank 19, the liquid storage tank 19 is fixedly connected to the outer wall of the box body 1, a first water pump is arranged in the liquid storage tank 19, and a flow valve is arranged on the flexible hose 18;

when liquid needs to be added, the first lead screw motor 23 is started, the piston end of the first air cylinder 22 descends to a specified height, the first lead screw motor 23 drives the first air cylinder 22 and the liquid adding head 17 to move to the position above a culture dish needing liquid adding, the piston end of the first air cylinder 22 is clamped with the elastic top cover and pushes the elastic top cover to open the liquid inlet of the culture dish and stop after reaching a specified position, the electromagnetic valve 62 on the liquid adding branch pipe 16 above the placing frame 5 where the culture dish is located is opened, other electromagnetic valves 62 are closed, the first water pump is started, nutrient liquid is added into the culture dish along the telescopic hose 18, the liquid adding main pipe 15, the liquid adding branch pipe 16 and the liquid adding head 17, the flow valve measures the adding flow, the first water pump is closed after the specified flow is reached, the amount of the added nutrient liquid is controlled, the first air cylinder 22 resets after the liquid adding is completed, and the elastic top cover resets; first cylinder 22 and liquid feeding are in charge of 16 and are set up side by side on bracing piece 25, mutual noninterference, through setting up first cylinder 22 and liquid feeding head 17 side by side, when first cylinder 22 pushes away the elasticity top cap, liquid feeding head 17 can just in time remove the liquid feeding mouth department of culture dish and carry out the liquid feeding, and easy operation is convenient.

According to a further optimized scheme, the mechanical clamping jaw comprises a second lead screw motor 26 fixedly connected to the outer wall of the box body 1, an output shaft of the second lead screw motor 26 extends into the taking cavity 3 and is provided with a second lead screw 27, and a second sliding sleeve 28 in sliding connection with the inner wall of the taking cavity 3 is slidably sleeved on the second lead screw 27;

a first stepping motor 29 is installed on the second sliding sleeve 28, a second air cylinder 30 is fixedly connected to an output shaft of the first stepping motor 29, a piston end of the second air cylinder 30 is fixedly connected with a third air cylinder 31, the second air cylinder 30 and the third air cylinder 31 are vertically arranged, a piston end of the third air cylinder 31 is fixedly connected with a clamping jaw motor 32, two clamping jaws 33 which are oppositely arranged are installed at an output end of the clamping jaw motor 32, and the two clamping jaws 33 are detachably connected with a culture dish;

when liquid needs to be added into the culture dish, the first stepping motor 29 is started to drive the clamping jaw 33 to reach a specified position, the second air cylinder 30 controls the clamping jaw 33 to reach a specified height, the third air cylinder 31 controls the clamping jaw 33 to stretch into the culture cavity 2, the clamping jaw motor 32 controls the two clamping jaws 33 to open and grab the culture dish, then the culture dish is conveyed to the position of the taking window 4, and when the culture dish on the placing frame 5 at the bottom layer needs to be grabbed, the first stepping motor 29 controls the second air cylinder 30 to rotate 180 degrees.

A plurality of fourth sliding chutes 34 arranged in parallel and a plurality of third sliding chutes 35 arranged opposite to the fourth sliding chutes 34 are formed on the inner side wall of the box body 1;

the electric sliding door comprises a third lead screw 38 which is rotatably connected in a third sliding chute 35, one end of the third lead screw 38 is fixedly connected with a third lead screw motor 39, the third lead screw motor 39 is fixedly connected on the outer wall of the box body 1, a third sliding block 37 which is in sliding connection with the third sliding chute 35 is slidably sleeved on the third lead screw 38, a sliding plate 36 is fixedly connected on the third sliding block 37, two adjacent sliding plates 36 are in sliding contact, and one end, far away from the third sliding block 37, of the sliding plate 36 is in sliding connection with a fourth sliding chute 34;

a sealing strip is arranged between two adjacent sliding plates 36, and after the sliding plates 36 are closed, the sealing property in the culture cavity 2 is improved; when needing to snatch the culture dish, need remove the slide 36 that corresponds with the rack 5 at culture dish place, third lead screw motor 39 starts, drives third slider 37 and slides, makes slide 36 remove, and slide 36 is opened, and the clamping jaw 33 can stretch into and snatch the culture dish in the culture chamber 2.

According to a further optimized scheme, a plurality of first air holes 40 are formed in the placing frame 5, and a limit stop 41 is fixedly connected to one side, far away from the clamping jaw 33, of the top surface of the placing frame 5;

the culture dish comprises a clamping shell 42, and the clamping shell 42 is placed on the placing frame 5 and clamped with the limit stop 41; the clamping jaw 33 is detachably connected with the clamping shell 42; the bottom of the clamping shell 42 is provided with a second air hole 43, the clamping shell 42 is internally clamped with a culture shell 44, the top of the culture shell 44 is in threaded connection with a culture cover 45, the culture cover 45 is fixedly connected with a liquid inlet pipe 6, and the liquid inlet pipe 6 is provided with an elastic top cover; a plurality of second grooves 70 are formed on the inner bottom wall of the culture shell 44; the culture shell 44 with the second groove 70 at the bottom is used in a matched manner according to the agglomeration growth characteristics of tumor cells, the culture shell 44 can be made of disposable materials or reusable materials such as glass, and the invention is not limited; the first air hole 40 and the second air hole 43 increase the contact area between the culture shell 44 and the air in the culture cavity 2, so that the proper temperature of the tumor cells in the culture shell 44 can be kept, and the normal growth of the tumor cells can be facilitated; when the culture shell 44 needs to be taken out, the clamping jaws 33 clamp the clamping shell 42, so that the damage caused by directly clamping the culture shell 44 is avoided; the limit stop 41 is arranged on one side close to the first sliding sleeve 7, so that the clamping shell 42 is not clamped by the clamping jaw 33, the clamping shell 42 can be positioned, and when the first air cylinder 22 pushes the elastic top cover to slide, the clamping shell 42 is prevented from shifting.

In a further preferred embodiment, the elastic top cover comprises:

the sleeve 10 is fixedly connected to the outer wall of the liquid inlet pipe 6; two first sliding grooves 11 are formed in the inner wall of the sleeve 10;

the top cover 8, the top cover 8 is connected with the top of the liquid inlet pipe 6 in a sliding way, and the liquid adding head 17 is positioned above the top cover 8;

a slide bar 12, the slide bar 12 is fixedly connected with one end of the top cover 8 close to the sleeve 10, two slide blocks 13 are fixedly connected with the side wall of the slide bar 12, the two slide blocks 13 are respectively connected with the two first slide grooves 11 in a sliding way,

the spring 14, the spring 14 is fixedly connected between the slide bar 12 and the sleeve 10;

the groove 9 is formed in one end, far away from the sliding rod 12, of the top cover 8; the groove 9 is clamped with the piston end of the first cylinder 22.

Further optimizing the scheme, the regulation and control system includes:

the temperature adjusting component is arranged on the culture cavity 2 and is used for adjusting the air temperature in the culture cavity 2;

the pressure regulating assembly is arranged on the culture cavity 2 and the taking cavity 3 and is used for regulating the air pressure in the culture cavity 2 and the taking cavity 3;

the humidifying component comprises a water storage bin 53 fixedly connected to the outer wall of the box body 1, a second water pump is fixedly connected in the water storage bin 53, a water outlet pipe 54 is fixedly connected on the water storage bin 53, the water outlet pipe 54 extends into the culture cavity 2 and is fixedly connected with a water distribution pipe 56, and a plurality of nozzles 57 are fixedly connected on the water distribution pipe 56; the nozzles 57 are positioned below the temperature adjusting assembly and above the placing frames 5; a humidity sensor 60 is arranged in the culture cavity 2;

an air outlet assembly; the air outlet component comprises an air outlet pipe 51 fixedly connected on the culture cavity 2, one end of the air outlet pipe 51 extends out of the culture cavity 2 and is fixedly connected with a one-way valve 52;

the temperature adjusting component comprises a cover shell 47 fixedly connected on the inner top wall of the culture cavity 2, the cover shell 47 is positioned above the nozzles 57, a plurality of electric heating plates 48 are embedded in the side wall of the cover shell 47, and the bottom of the cover shell 47 is provided with third air holes 49; a temperature sensor 61 is arranged in the culture cavity 2;

the pressure regulating component comprises two air pumps 58 fixedly connected on the outer wall of the box body 1, and the air outlet ends of the two air pumps 58 are respectively communicated with the taking cavity 3 and the inner cavity of the housing 47; an air filter element is arranged at the air inlet end of the air pump 58; the taking cavity 3 and the culture cavity 2 are both internally provided with air pressure sensors 50;

the disinfection component comprises a plurality of ultraviolet disinfection lamps 59 fixedly connected on the inner wall of the culture chamber 2 and the inner wall of the taking chamber 3;

the air pump 58 supplies filtered air into the housing 47, the electric heating plate 48 heats the air in the housing 47, the heated air is blown into the culture cavity 2 through the third air hole 49, the temperature sensor 61 detects the temperature in the culture cavity 2, and the temperature in the culture cavity 2 is controlled by controlling the opening and closing of the electric heating plate 48; the air pump 58 blows air to accelerate air circulation in the culture cavity 2, so that the temperature and the humidity of each part in the culture cavity 2 are kept consistent;

the water storage bin 53 supplies water vapor into the culture cavity 2 through a nozzle 57 to keep the humidity in the culture cavity 2, and the nozzle 57 is positioned below the housing 47 to blow the moisture by the gas out of the housing 47 to accelerate the circulation; through holes are formed in the placing frames 5 below the nozzles 57, so that the flow of hot air and moisture among the placing frames 5 in each layer is accelerated, and the temperature and the humidity among the placing frames 5 are kept consistent quickly; the ultraviolet disinfection lamp 59 is used for sterilizing and disinfecting the taking cavity 3 and the culture cavity 2.

When the tumor cell culture device is used, the sliding plate 36 is opened, and the culture shell 44 is placed on the placing frame 5 through the clamping jaw 33; when liquid is added, the first lead screw 24 is started to drive the first air cylinder 22 and the liquid adding head 17 to move, the first air cylinder 22 pushes the top cover 8, and the first water pump is started to add liquid; when the culture housing 44 is taken out, the slide plate 36 is opened, the holding jaws 33 hold the holding case 42 and move to the taking chamber 3, the slide plate 36 is closed, and the taking window 4 is opened to take out the culture housing.

Example 2

Referring to fig. 7-8, the difference from embodiment 1 is that a water tank 63 is fixedly connected to the outer wall of the box body 1, a third water pump (not shown) is installed in the water tank 63, a cleaning pipe 64 is fixedly connected to the water tank 63, and one end of the cleaning pipe 64 far away from the water tank 63 is communicated with the flexible hose 18 through a three-way valve 65; the bottom of the culture cavity 2 is fixedly connected with a collecting box 20, the collecting box 20 is positioned right below the placing rack 5, the collecting box 20 is fixedly connected with a water outlet pipe 66, and the water outlet pipe 66 penetrates through the bottom wall of the culture cavity 2 and extends out of the culture cavity 2; the water outlet pipe 66 is fixedly connected with a water outlet valve 55;

after the cultivation, the cleaning liquid is added into the water tank 63, during cleaning, the three-way valve 65 is adjusted to enable the flexible hose 18 to be communicated with the cleaning pipe 64, the inner cavities of the flexible hose 18, the main liquid adding pipe 15, the branch liquid adding pipe 16 and the liquid adding head 17 are cleaned and disinfected through the cleaning liquid, bacterial infection is prevented, and the cleaning liquid flowing out of the liquid adding head 17 flows into the collecting box 20 at the bottom through the first air holes 40 in the placing frame 5 and is discharged through the water outlet pipe 66.

Example 3

Referring to FIG. 9, the difference from example 1 is that a buffer layer 68 is fixed to the inner wall of the culture shell 44, and a gap is provided between the buffer layer 68 and the inner top of the culture shell 44; the buffer layer 68 is fixedly connected with a stop block 67, the stop block 67 is positioned right below the liquid inlet pipe 6, and the buffer layer 68 is provided with a plurality of circulation holes 69;

when the nutrient solution added into feed liquor pipe 6, dog 67 cushions the nutrient solution, reduces the impact force of nutrient solution, reduces the impact to tumor cell, does benefit to the completeness that keeps tumor cell, does benefit to tumor cell normal growth, and the nutrient solution scatters all around under the effect of dog 67, evenly falls into buffer layer 68 to flow into through circulation hole 69 and cultivate the casing 44 in, accelerate the diffusion of nutrient solution.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. A tumor cell culture apparatus, comprising:

the disinfection box comprises a box body (1), wherein a disinfection component is installed on the box body (1), a plurality of electric push doors are installed in the box body (1), and the electric push doors divide the interior of the box body (1) into a culture cavity (2) and a taking cavity (3); a taking window (4) is arranged on the taking cavity (3);

the culture device comprises a plurality of placing frames (5), wherein the placing frames (5) are fixedly connected in the culture cavity (2) at intervals up and down, a plurality of culture dishes are detachably connected to the placing frames (5), the liquid inlet ends of the culture dishes are connected with elastic top covers in a sliding mode, and a plurality of second grooves (70) are formed in the bottoms of the culture dishes;

the movable liquid adding mechanism is arranged on the culture cavity (2) and is used for adding liquid into a plurality of culture dishes; the output end of the movable liquid adding mechanism is provided with a lifting rod for controlling the opening and closing of the elastic top cover;

the mechanical clamping jaw is arranged in the taking cavity (3) and is used for taking, placing and transferring the culture dish;

the regulation and control system is installed on the box body (1) and used for regulating and controlling greenhouse, humidity, wind pressure and the wind pressure in the culture cavity (2) and the taking cavity (3).

2. The tumor cell culture apparatus according to claim 1, wherein: the movable liquid adding mechanism comprises a first lead screw motor (23) fixedly connected to the outer wall of the box body (1), an output shaft of the first lead screw motor (23) extends into the culture cavity (2) and is provided with a first lead screw (24), and a first sliding sleeve (7) in sliding connection with the bottom of the culture cavity (2) is slidably sleeved on the first lead screw (24); a supporting rod (25) is fixedly connected to the first sliding sleeve (7), and a liquid adding assembly and the lifting rod are fixedly connected to the supporting rod (25);

the lifting rod comprises a plurality of supporting rods (21) fixedly connected to the supporting rod (25), the supporting rods (21) and the placing frames (5) are arranged in a one-to-one correspondence mode, first air cylinders (22) are fixedly connected to the bottoms of the supporting rods (21), and piston ends of the first air cylinders (22) are detachably connected with the elastic top cover; the liquid feeding assembly is used for feeding liquid into the culture dishes.

3. The tumor cell culture apparatus according to claim 2, wherein: the liquid adding assembly comprises a liquid adding main pipe (15) fixedly connected to the supporting rod (25), a plurality of liquid adding branch pipes (16) are fixedly connected to the liquid adding main pipe (15), the liquid adding branch pipes (16) are arranged in one-to-one correspondence with the placing frames (5), a liquid adding head (17) and an electromagnetic valve (62) are fixedly connected to the liquid adding branch pipes (16), and the liquid adding head (17) is located above the elastic top cover; keep away from main liquid feeding pipe (15) the one end rigid coupling of liquid feeding branch pipe (16) has expansion hose (18), expansion hose (18) stretch out to cultivate chamber (2) outer and rigid coupling has liquid reserve tank (19), liquid reserve tank (19) rigid coupling is in on box (1) outer wall, install first water pump in liquid reserve tank (19), install the flow valve on expansion hose (18).

4. The tumor cell culture apparatus of claim 3, wherein: the mechanical clamping jaw comprises a second lead screw motor (26) fixedly connected to the outer wall of the box body (1), an output shaft of the second lead screw motor (26) extends into the taking cavity (3) and is provided with a second lead screw (27), and a second sliding sleeve (28) in sliding connection with the inner wall of the taking cavity (3) is slidably sleeved on the second lead screw (27);

install first step motor (29) on second sliding sleeve (28), the rigid coupling has second cylinder (30) on the output shaft of first step motor (29), the piston end rigid coupling of second cylinder (30) has third cylinder (31), second cylinder (30) with third cylinder (31) set up perpendicularly, the piston end rigid coupling of third cylinder (31) has clamping jaw motor (32), two clamping jaw (33) that set up relatively are installed to the output of clamping jaw motor (32), two clamping jaw (33) with the connection can be dismantled to the culture dish.

5. The tumor cell culture apparatus according to claim 1, wherein: a plurality of fourth sliding grooves (34) which are arranged in parallel and a plurality of third sliding grooves (35) which are arranged opposite to the fourth sliding grooves (34) are formed in the inner side wall of the box body (1);

electric sliding door is connected including rotating third lead screw (38) in third spout (35), third lead screw (38) one end rigid coupling has third lead screw motor (39), third lead screw motor (39) rigid coupling is in on box (1) outer wall, sliding sleeve is equipped with on third lead screw (38) with third spout (35) sliding connection's third slider (37), the rigid coupling has slide (36) on third slider (37), adjacent two sliding contact between slide (36), slide (36) are kept away from the one end of third slider (37) with fourth spout (34) sliding connection.

6. The tumor cell culture apparatus according to claim 4, wherein: a plurality of first air holes (40) are formed in the placing frame (5), and a limit stop (41) is fixedly connected to one side, far away from the clamping jaw (33), of the top surface of the placing frame (5);

the culture dish comprises a clamping shell (42), and the clamping shell (42) is placed on the placing frame (5) and clamped with the limit stop (41); the clamping jaw (33) is detachably connected with the clamping shell (42); the bottom of the clamping shell (42) is provided with a second air hole (43), a culture shell (44) is clamped in the clamping shell (42), the top of the culture shell (44) is in threaded connection with a culture cover (45), a liquid inlet pipe (6) is fixedly connected to the culture cover (45), and the elastic top cover is installed on the liquid inlet pipe (6); the inner bottom wall of the culture shell (44) is provided with a plurality of second grooves (70).

7. The tumor cell culture apparatus of claim 6, wherein: the elastic top cover comprises:

the sleeve (10), the said sleeve (10) is affixed to the outer wall of the said liquid inlet pipe (6); two first sliding grooves (11) are formed in the inner wall of the sleeve (10);

the top cover (8), the top cover (8) and the top of the liquid inlet pipe (6) are connected in a sliding mode, and the liquid adding head (17) is located above the top cover (8);

the sliding rod (12), the sliding rod (12) is fixedly connected with one end of the top cover (8) close to the sleeve (10), two sliding blocks (13) are fixedly connected to the side wall of the sliding rod (12), the two sliding blocks (13) are respectively in sliding connection with the two first sliding grooves (11),

the spring (14), the said spring (14) is affixed between said slide bar (12) and said bush (10);

the groove (9), the groove (9) is arranged at one end of the top cover (8) far away from the sliding rod (12); the groove (9) is clamped with the piston end of the first cylinder (22).

8. The tumor cell culture apparatus according to claim 1, wherein: the regulation and control system comprises:

the temperature adjusting component is arranged on the culture cavity (2) and is used for adjusting the air temperature in the culture cavity (2);

the pressure regulating assembly is arranged on the culture cavity (2) and the taking cavity (3) and is used for regulating the air pressure in the culture cavity (2) and the taking cavity (3);

the humidifying component comprises a water storage bin (53) fixedly connected to the outer wall of the box body (1), a second water pump is fixedly connected in the water storage bin (53), a water outlet pipe (54) is fixedly connected to the water storage bin (53), the water outlet pipe (54) extends into the culture cavity (2) and is fixedly connected with a water distribution pipe (56), and a plurality of nozzles (57) are fixedly connected to the water distribution pipe (56); the plurality of nozzles (57) are positioned below the temperature adjusting assembly and above the plurality of placing frames (5); a humidity sensor (60) is arranged in the culture cavity (2);

an air outlet assembly; the air outlet assembly comprises an exhaust pipe (51) fixedly connected to the culture cavity (2), one end of the exhaust pipe (51) extends out of the culture cavity (2) and is fixedly connected with a one-way valve (52).

9. The tumor cell culture apparatus of claim 8, wherein: the temperature adjusting component comprises a cover shell (47) fixedly connected to the inner top wall of the culture cavity (2), the cover shell (47) is positioned above the plurality of nozzles (57), a plurality of electric heating plates (48) are embedded in the side wall of the cover shell (47), and a third air hole (49) is formed in the bottom of the cover shell (47); a temperature sensor (61) is arranged in the culture cavity (2);

the pressure regulating assembly comprises two air pumps (58) fixedly connected to the outer wall of the box body (1), and the air outlet ends of the two air pumps (58) are respectively communicated with the taking cavity (3) and the inner cavity of the housing (47); an air filter element is mounted at the air inlet end of the air pump (58); air pressure sensors (50) are arranged in the taking cavity (3) and the culture cavity (2).

10. The tumor cell culture apparatus according to claim 1, wherein: the disinfection component comprises a plurality of ultraviolet disinfection lamps (59) fixedly connected to the inner wall of the culture chamber (2) and the inner wall of the taking chamber (3).