CN114806873A

CN114806873A - Device for culturing islet cells in islet transplantation

Info

Publication number: CN114806873A
Application number: CN202210560311.8A
Authority: CN
Inventors: 何莹
Original assignee: West China Hospital of Sichuan University
Current assignee: West China Hospital of Sichuan University
Priority date: 2022-05-23
Filing date: 2022-05-23
Publication date: 2022-07-29

Abstract

The invention provides a device for culturing islet cells in islet transplantation, and relates to the field of islet transplantation. The device for culturing the islet cells in the islet transplantation comprises a base, wherein a connecting frame is in sliding connection with a side frame, and a pressure bar assembly is arranged between a rack bar and a box body. The device for culturing the islet cells in the islet transplantation drives the output shaft to rotate through the output end of the motor, so that the rotating plate rotates ninety degrees at the opening in the middle of the partition plate, and the guy rope drives the piston disc to simultaneously slide between the partition plate and the wall of the box body, and the timed automatic air supplement for the interior of the box body can be realized, so that the oxygen content in the box body can be kept in a proper state under a closed temperature control state; the temperature sensing of the dispersed fins is conveyed to the inner part of the bar-shaped cavity through the temperature conducting strip, the built-in slider of the bar drives the thin rod to slide relative to the vertical cavity, and the contact piece at the top end of the thin rod slides to be abutted against the long contact strip, so that the refrigeration strip works to refrigerate and adjust the ambient temperature in the box body.

Description

Device for culturing islet cells in islet transplantation

Technical Field

The invention relates to the field of islet transplantation, in particular to a device for culturing islet cells in islet transplantation.

Background

The islet transplantation greatly promotes the relevant research of diabetes treatment, has important treatment value for insulin-dependent diabetes mellitus and diabetes complicated end-stage nephropathy, enables patients not to depend on exogenous insulin any more, reduces or delays the occurrence and development of complications, and greatly improves the life quality and clinical outcome of the patients. However, islet transplantation has many difficulties, which restrict its wide clinical application. The islet cell culture device is generally used for islet cell culture in islet transplantation.

Most of the existing devices for culturing the islet cells in the islet transplantation have the same structure, and because the suitable culture conditions of the islet cells require the temperature of the culture environment, the temperature of the islet cells needs to be constantly ensured to be in a stable and suitable state, and the temperature in the culture device is constantly monitored and adjusted by workers, so that the workload of the culture work is greatly increased; in the process of culturing the islet cells, in order to ensure that the temperature is suitable for cell culture at any time, the culture device is required to be in a closed state, so that the fluctuation of the external temperature on the temperature inside the culture device due to certain influence is avoided, the oxygen content of the air inside the device in the closed state is gradually reduced, and the influence on the cell culture is caused to a certain extent.

Therefore, there is a need to provide a new device for islet cell culture in islet transplantation to solve the above-mentioned technical problems.

Disclosure of Invention

In order to solve the technical problem, the invention provides a device for culturing islet cells in islet transplantation.

The invention provides a device for culturing islet cells in islet transplantation, which comprises: the refrigerator comprises a base, wherein one end of the base is fixedly connected with a side frame, the base is fixedly connected with a box body, one side of the box body is provided with a box door, the interior of the box body is fixedly connected with a partition plate, the middle part of the partition plate is provided with a rotating plate, the lower end of the box body is fixedly connected with a placing seat, the top end of the side frame is connected with a motor in an installing manner, the outer wall of the box body is connected with a timing controller in an installing manner, the lower end face of the partition plate is connected with a refrigerating strip in an installing manner, and the lower end face of the partition plate is fixedly connected with a porous barrel frame; supplementary mechanism of admitting air, supplementary mechanism of admitting air includes the embedded pole, embedded pole and rotor plate fixed connection, the one end fixedly connected with gear of embedded pole, be equipped with the lug on the lateral wall of box, sliding connection has the rack bar on the lug, the gear meshes with the rack bar mutually, fixedly connected with link on the rack bar, the output fixedly connected with output shaft of motor, the middle part of output shaft is equipped with the screw thread, the middle part and the output shaft threaded connection of link, link and side bearer sliding connection, be equipped with the pressure row subassembly between rack bar and the box.

Preferably, the middle part of division board is equipped with the opening, be equipped with the screens groove on the open-ended inner circle wall, the one end and the screens groove of embedded pole rotate to be connected, the relative one side in screens groove is equipped with and runs through the groove, the embedded pole with run through the groove and rotate to be connected.

Preferably, the pressure-discharge assembly comprises an upper guide wheel, the upper guide wheel is fixedly installed on the outer wall of the box body, the upper end of the rack rod is connected with a traction rope, the traction rope is attached to the upper guide wheel, the traction rope is connected with the box wall of the box body in a sliding mode, a lower guide wheel is installed on the inner wall of the box body, the traction rope is attached to the lower guide wheel, a pull rod is connected to the side wall of the box body in a sliding mode, a piston disc is fixedly connected to one end of the pull rod, a spring is sleeved on the pull rod, one end of the spring is connected with the pull rod, and the other end of the spring is connected with the outer wall of the box body.

Preferably, be equipped with the automatic constant temperature mechanism on the inner wall of box, the automatic constant temperature mechanism includes dispersion fin, dispersion fin fixed mounting is on the inner wall of box, be equipped with the bar chamber on the lateral wall of box, fixedly connected with leads the temperature strip on the dispersion fin, the one end of leading the temperature strip extends to in the bar chamber, be equipped with built-in slider in the bar chamber, fixedly connected with slender pole on the built-in slider, be equipped with inflation liquid between built-in slider and the bar chamber, the upper end in bar chamber is equipped with perpendicular chamber, the upper end of slender pole extends to the inside of perpendicular chamber, the top fixedly connected with contact piece of slender pole, the chamber wall mounting in perpendicular chamber is connected with long touch strip.

Preferably, the lower end of the box body is provided with an exhaust port, and an exhaust valve is installed and connected at the exhaust port.

Preferably, the upper end of box is equipped with circular mouthful, circular mouthful department erection joint has the admission valve, circular mouthful department is equipped with the filter screen.

Preferably, the side frame is provided with a touch time delay switch, the piston disc is provided with a magnetic block in an embedded mode, the top of the box body is internally provided with an electromagnet in an embedded mode, and the magnetic pole of the magnetic block is opposite to that of the electromagnet.

Compared with the related art, the device for culturing the islet cells in the islet transplantation has the following beneficial effects:

1. the invention provides a device for culturing islet cells in islet transplantation, wherein an output shaft is driven by an output end of a motor to rotate, so that a rotating plate rotates ninety degrees at an opening in the middle of a partition plate, and a pulling rope drives a piston disc to simultaneously slide between the partition plate and the wall of a box body, so that the interior of the box body can be automatically supplemented with air at regular time, and the oxygen content in the box body can be kept in a proper state under a closed temperature control state;

2. the invention provides a device for culturing islet cells in islet transplantation, which is characterized in that dispersed fin temperature sensing is conveyed to the inside of a strip-shaped cavity through a temperature conducting strip, a strip built-in slide piece drives a thin rod to slide relative to a vertical cavity, a contact piece at the top end of the thin rod slides to be abutted against a long contact strip, so that a refrigeration strip can work, and the ambient temperature in a box body can be automatically refrigerated and adjusted.

Drawings

FIG. 1 is a schematic structural diagram of an apparatus for islet cell culture in islet transplantation according to a preferred embodiment of the present invention;

FIG. 2 is a schematic structural diagram of an auxiliary air intake mechanism of the auxiliary air intake mechanism shown in FIG. 1;

FIG. 3 is a schematic structural view of the automatic thermostatic mechanism shown in FIG. 1;

FIG. 4 is a schematic view of the structure shown at A in FIG. 1;

FIG. 5 is a schematic view of the structure shown at B in FIG. 1;

fig. 6 is a schematic structural view at C shown in fig. 1.

Reference numbers in the figures: 1. a base; 11. a side frame; 111. a touch delay switch; 2. a box body; 21. a box door; 22. a partition plate; 23. a rotating plate; 24. a placing seat; 25. an electromagnet; 3. a motor; 4. a timing controller; 5. refrigerating the strips; 51. a porous cartridge holder; 6. an auxiliary air intake mechanism; 61. a rod is embedded; 611. a gear; 62. a rack bar; 63. a connecting frame; 64. an output shaft; 65. an upper guide wheel; 66. a pulling rope; 67. a lower guide wheel; 68. a pull rod; 681. a piston disc; 6811. a magnetic block; 682. a spring; 7. an automatic constant temperature mechanism; 71. dispersing fins; 72. a heat conducting strip; 73. a built-in slider; 74. a thin rod; 75. an expansion liquid; 76. A contact piece; 77. a long touch bar; 8. an exhaust valve; 9. an intake valve; 91. and (5) filtering by using a filter screen.

Detailed Description

The invention is further explained below with reference to the drawings and the embodiments.

Referring to fig. 1 to 6, an apparatus for culturing islet cells in islet transplantation includes: the refrigerator comprises a base 1, wherein one end of the base 1 is fixedly connected with a side frame 11, the base 1 is fixedly connected with a box body 2, one side of the box body 2 is provided with a box door 21, the inside of the box body 2 is fixedly connected with a partition plate 22, the middle part of the partition plate 22 is provided with a rotating plate 23, the lower end of the box body 2 is fixedly connected with a placing seat 24, the top end of the side frame 11 is fixedly connected with a motor 3, the outer wall of the box body 2 is fixedly connected with a timing controller 4, the lower end face of the partition plate 22 is fixedly connected with a refrigerating strip 5, and the lower end face of the partition plate 22 is fixedly connected with a porous barrel frame 51; the auxiliary air inlet mechanism 6 is characterized in that the auxiliary air inlet mechanism 6 comprises an embedded rod 61, the embedded rod 61 is fixedly connected with the rotating plate 23, one end of the embedded rod 61 is fixedly connected with a gear 611, a convex block is arranged on the side wall of the box body 2, a rack rod 62 is connected on the convex block in a sliding mode, the gear 611 is meshed with the rack rod 62, a connecting frame 63 is fixedly connected onto the rack rod 62, an output end of the motor 3 is fixedly connected with an output shaft 64, threads are arranged in the middle of the output shaft 64, the middle of the connecting frame 63 is connected with the output shaft 64 in a threaded mode, the connecting frame 63 is connected with the side frame 11 in a sliding mode, and a pressure row assembly is arranged between the rack rod 62 and the box body 2.

In the specific implementation process, as shown in fig. 1 and 2, an opening is formed in the middle of the partition plate 22, a clamping groove is formed in the inner ring wall of the opening, one end of the embedded rod 61 is rotatably connected with the clamping groove, a through groove is formed in the side, opposite to the clamping groove, of the embedded rod 61, and the embedded rod 61 is rotatably connected with the through groove.

It should be noted that: the rotating plate 23 rotates ninety degrees at the opening in the middle of the partition plate 22, and the rotating plate 23 is vertical relative to the opening at this time, that is, the opening can be opened.

Referring to fig. 1, the pressure-discharge assembly includes an upper guide wheel 65, the upper guide wheel 65 is fixedly mounted on the outer wall of the box body 2, the upper end of the rack rod 62 is connected with a guy rope 66, the guy rope 66 is attached to the upper guide wheel 65, the guy rope 66 is connected to the wall of the box body 2 in a sliding manner, a lower guide wheel 67 is mounted on the inner wall of the box body 2, the guy rope 66 is attached to the lower guide wheel 67, a pull rod 68 is slidably connected to the side wall of the box body 2, a piston disc 681 is fixedly connected to one end of the pull rod 68, a spring 682 is sleeved on the pull rod 68, one end of the spring 682 is connected to the pull rod 68, and the other end of the spring 682 is connected to the outer wall of the box body 2.

It should be noted that: the downward movement of the rack bar 62 drives the pulling rope 66 to move along with the pulling rope, the movement of the pulling rope 66 enables the upper guide wheel 65 at the upper end of the box body 2 and the lower guide wheel 67 in the box body 2 to rotate simultaneously, namely, the other end of the pulling rope 66 drives the piston disc 681 to slide between the partition plate 22 and the box wall of the box body 2 simultaneously, the movement of the piston disc 681 drives the pull rod 68 to slide relative to the side wall of the box body 2, the spring 682 is elastically deformed along with the spring 682, and the movement of the piston disc 681 presses a part of air above the partition plate 22 to a space below the partition plate 22 through the opening to complete air supplement.

Referring to fig. 1 and 2, an automatic constant temperature mechanism 7 is arranged on the inner wall of the box body 2, the automatic constant temperature mechanism 7 includes a dispersion fin 71, the dispersion fin 71 is fixedly mounted on the inner wall of the box body 2, a strip-shaped cavity is arranged on the side wall of the box body 2, a temperature conducting strip 72 is fixedly connected to the dispersion fin 71, one end of the temperature conducting strip 72 extends into the strip-shaped cavity, a built-in slider 73 is arranged in the strip-shaped cavity, a thin rod 74 is fixedly connected to the built-in slider 73, an expansion liquid 75 is arranged between the built-in slider 73 and the strip-shaped cavity, a vertical cavity is arranged at the upper end of the strip-shaped cavity, the upper end of the thin rod 74 extends into the vertical cavity, a contact 76 is fixedly connected to the top end of the thin rod 74, an automatic constant temperature mechanism 7 is arranged on the inner wall of the vertical cavity, a long contact strip 77 is fixedly connected to the inner wall of the box body 2, the automatic constant temperature mechanism 7 includes a dispersion fin 71, the dispersion fin 71 is fixedly mounted on the inner wall of the box body 2, the side wall of the box body 2 is provided with a strip-shaped cavity, the dispersing fins 71 are fixedly connected with temperature conducting strips 72, one ends of the temperature conducting strips 72 extend into the strip-shaped cavity, a built-in slider 73 is arranged in the strip-shaped cavity, thin rods 74 are fixedly connected to the built-in slider 73, expansion liquid 75 is arranged between the built-in slider 73 and the strip-shaped cavity, the upper end of the strip-shaped cavity is provided with a vertical cavity, the upper end of each thin rod 74 extends into the vertical cavity, the top end of each thin rod 74 is fixedly connected with a contact sheet 76, and long contact strips 77 are installed on the wall of the vertical cavity.

It should be noted that: the temperature sensed by the dispersion fin 71 is transmitted to the inside of the bar-shaped cavity through the temperature conducting strip 72, so that the temperature of expansion liquid 75 (low boiling point solution with temperature change morphological characteristics) in the bar-shaped cavity is changed, the volume of the expansion liquid 75 is changed to push the built-in slide piece 73 to slide upwards in the bar-shaped cavity, the upper end of the thin rod 74 is driven to slide relative to the vertical cavity, the contact piece 76 at the top end of the thin rod 74 slides to be abutted against the long contact strip 77, and a complete electric path is formed to enable the refrigeration strip piece 5 to automatically work;

when the contact 76 slides to abut against the long contact bar 77, an electric path is formed between the contact 76, the long contact bar 77, the refrigeration strip 5 and an external power supply.

Referring to fig. 1, the lower end of the box body 2 is provided with an exhaust port, and an exhaust valve 8 is installed and connected at the exhaust port.

It should be noted that: the setting of gas vent can guarantee that the inside atmospheric pressure of box 2 is stable.

Referring to fig. 1 and 3, the upper end of the box body 2 is provided with a circular opening, the circular opening is provided with an air inlet valve 9, and the circular opening is provided with a filter screen 91.

It should be noted that: the arrangement of the air inlet valve 9 can ensure that the air can be automatically stored after the piston disc 681 is reset;

the filter screen 91 is provided to filter dust from air introduced into the interior of the cabinet 2.

Referring to fig. 1 and 3, a touch delay switch 111 is mounted and connected on the side frame 11, a magnetic block 6811 is mounted on the piston disk 681 in an embedded manner, an electromagnet 25 is mounted on the top inside of the case 2 in an embedded manner, and the magnetic block 6811 is opposite to the magnetic pole of the electromagnet 25.

It should be noted that: the upper end of the rack rod 62 moves and abuts against the touch delay switch 111, the electromagnet 25 is controlled to be magnetic within a period of time, the magnetic block 6811 on the piston disc 681 is adsorbed, and the position of the piston disc 681 is kept unchanged within a period of time.

The working principle of the device for culturing the islet cells in the islet transplantation provided by the invention is as follows: if the internal temperature of the box body 2 cannot reach the culture temperature of the islet cells, the temperature sensed by the dispersing fins 71 is transmitted to the inside of the strip-shaped cavity through the temperature conducting strips 72, so that the temperature of the expansion liquid 75 (low-boiling-point solution with a temperature change form) in the strip-shaped cavity is changed, the volume of the expansion liquid 75 is changed to push the built-in slide piece 73 to slide upwards in the strip-shaped cavity, the upper end of the thin rod 74 is driven to slide relative to the vertical cavity, the contact piece 76 at the top end of the thin rod 74 slides to abut against the long contact strip 77, and a complete electric path is formed to enable the refrigerating strip piece 5 to automatically work, so that the internal ambient temperature of the box body 2 is adjusted in a refrigerating mode.

The timing controller 4 controls the motor 3 to work at a timing, the output end of the motor 3 drives the output shaft 64 to rotate, so that the connecting frame 63 slides relative to the side frame 11, one end of the connecting frame 63 drives the rack rod 62 to move along with the rack rod, the gear 611 meshed with the rack at the lower end of the rack rod 62 rotates simultaneously, the rotation of the gear 611 drives the embedded rod 61 to rotate along with the rack rod, the rotating plate 23 rotates ninety degrees at the opening in the middle of the partition plate 22, the rotating plate 23 is in a vertical state relative to the opening at the moment, and the opening is in an open state; the pulling rope 66 is driven by the downward movement of the rack bar 62 to move, the upper guide wheel 65 at the upper end of the box body 2 and the lower guide wheel 67 in the box body 2 rotate simultaneously by the movement of the pulling rope 66, so that the other end of the pulling rope 66 drives the piston disc 681 to slide between the partition plate 22 and the box wall of the box body 2, the movement of the piston disc 681 drives the pull rod 68 to slide relative to the side wall of the box body 2, the spring 682 elastically deforms accordingly, the movement of the piston disc 681 presses a part of air above the partition plate 22 to the space below the partition plate 22 through the opening, the air is uniformly discharged through a plurality of round holes at the lower end of the porous barrel frame 51, the upper end of the rack bar 62 moves and abuts against the touch delay switch 111, the electromagnet 25 is controlled to have magnetism in a period of time to adsorb the magnetic block 6811 on the piston disc 681, the position of the piston disc 681 is kept unchanged for a period of time, if the culture temperature is not proper and the refrigeration strip 5 works, the temperature of the entering air can be pretreated under the air supplementing state, and the change of the culture temperature in the box body 2 cannot be influenced to a good degree;

the air in the space below the partition plate 22 is increased, so that the air pressure is increased, and the air in the space can be automatically discharged through the exhaust valve 8 at the lower end of the box body 2; and the output end of the motor 3 rotates reversely after air supplement is finished, so that the pulling rope 66 moves reversely, and when the rotating plate 23 rotates ninety degrees reversely at the opening in the middle of the partition plate 22, so that the opening is in a closed state, the electromagnet 25 loses magnetism and is separated from the magnetic block 6811, the spring 682 recovers elastic deformation, the piston disc 681 moves reversely, external air flows in automatically through the air inlet valve 9, and automatic air storage is realized.

The above description is only an embodiment of the present invention, and is not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes performed by the present specification and the attached drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. An apparatus for islet cell culture in islet transplantation, comprising:

the refrigerator comprises a base (1), wherein one end of the base (1) is fixedly connected with a side frame (11), a box body (2) is fixedly connected onto the base (1), a box door (21) is arranged on one side of the box body (2), a partition plate (22) is fixedly connected into the box body (2), a rotating plate (23) is arranged in the middle of the partition plate (22), a placing seat (24) is fixedly connected to the lower end of the box body (2), a motor (3) is fixedly connected to the top end of the side frame (11), a timing controller (4) is fixedly connected to the outer wall of the box body (2), a refrigerating strip (5) is fixedly connected to the lower end face of the partition plate (22), and a porous barrel frame (51) is fixedly connected to the lower end face of the partition plate (22);

supplementary mechanism (6) of admitting air, supplementary mechanism (6) of admitting air includes embedded bar (61), embedded bar (61) and rotor plate (23) fixed connection, the one end fixedly connected with gear (611) of embedded bar (61), be equipped with the lug on the lateral wall of box (2), sliding connection has rack bar (62) on the lug, gear (611) and rack bar (62) mesh mutually, fixedly connected with link (63) on rack bar (62), the output end fixedly connected with output shaft (64) of motor (3), the middle part of output shaft (64) is equipped with the screw thread, the middle part and output shaft (64) threaded connection of link (63), link (63) and side bearer (11) sliding connection, be equipped with the pressure row subassembly between rack bar (62) and box (2).

2. The device for culturing the islet cells in the islet transplantation as recited in claim 1, wherein an opening is formed in the middle of the partition plate (22), a retaining groove is formed in an inner circumferential wall of the opening, one end of the embedded rod (61) is rotatably connected with the retaining groove, a through groove is formed in the opposite side of the retaining groove, and the embedded rod (61) is rotatably connected with the through groove.

3. The apparatus for islet cell culture in islet transplantation according to claim 1, the pressure bar component comprises an upper guide wheel (65), the upper guide wheel (65) is fixedly arranged on the outer wall of the box body (2), the upper end of the rack bar (62) is connected with a guy rope (66), the guy rope (66) is jointed with the upper guide wheel (65), the pulling rope (66) is connected with the wall of the box body (2) in a sliding way, the inner wall of the box body (2) is provided with a lower guide wheel (67), the pulling rope (66) is attached to the lower guide wheel (67), the side wall of the box body (2) is connected with a pull rod (68) in a sliding way, one end of the pull rod (68) is fixedly connected with a piston disc (681), a spring (682) is sleeved on the pull rod (68), one end of the spring (682) is connected with the pull rod (68), and the other end of the spring (682) is connected with the outer wall of the box body (2).

4. The device for culturing the islet cells in the islet transplantation according to claim 1, wherein an automatic constant temperature mechanism (7) is arranged on the inner wall of the box body (2), the automatic constant temperature mechanism (7) comprises a dispersion fin (71), the dispersion fin (71) is fixedly installed on the inner wall of the box body (2), a strip-shaped cavity is arranged on the side wall of the box body (2), a temperature guide strip (72) is fixedly connected to the dispersion fin (71), one end of the temperature guide strip (72) extends into the strip-shaped cavity, an internal slider (73) is arranged in the strip-shaped cavity, a thin rod (74) is fixedly connected to the internal slider (73), an expansion liquid (75) is arranged between the internal slider (73) and the strip-shaped cavity, a vertical cavity is arranged at the upper end of the strip-shaped cavity, the upper end of the thin rod (74) extends into the vertical cavity, and a contact piece (76) is fixedly connected to the top end of the thin rod (74), the cavity wall of the vertical cavity is provided with a long touch strip (77).

5. The device for culturing the islet cells in the islet transplantation according to claim 1, wherein an exhaust port is formed at the lower end of the box body (2), and an exhaust valve (8) is installed and connected to the exhaust port.

6. The device for culturing the islet cells in the islet transplantation as recited in claim 1, wherein a circular opening is formed in the upper end of the box body (2), an air inlet valve (9) is connected to the circular opening in an installing mode, and a filter screen (91) is arranged at the circular opening.

7. The device for culturing the islet cells in the islet transplantation as claimed in claim 3, wherein the side frame (11) is provided with a touch delay switch (111), the piston disc (681) is provided with a magnetic block (6811) in an embedded manner, the top inside of the box body (2) is provided with an electromagnet (25) in an embedded manner, and the magnetic block (6811) and the electromagnet (25) have opposite magnetic poles.