CN112410205A

CN112410205A - Biological cell culture device with micro-vibration stress stimulation

Info

Publication number: CN112410205A
Application number: CN202011520789.5A
Authority: CN
Inventors: 郭世建
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-12-21
Filing date: 2020-12-21
Publication date: 2021-02-26

Abstract

The invention discloses a biological cell culture device with micro-vibration stress stimulation, which comprises a box body with an opening at the upper end. The invention is provided with a front shaft provided with a ratchet wheel, a middle shaft provided with a handle and a limit bolt which can limit a push rod, and a movable latch which is movably propped against the ratchet wheel is arranged in a box body, and a bearing plate is utilized to provide continuous support which can move up and down, so that a culture medium can vibrate in a stable vertical direction; the first-stage fixed magnetic block, the second-stage fixed magnetic block and the movable magnetic block which have the same poles repelling each other are arranged in the bearing plate, and the movable magnetic block is used for providing a movable support which can move horizontally for a culture medium, so that the culture medium can be subjected to stress stimulation; a cover plate connected with the rack is driven by a five-stage gear on the vertical shaft to horizontally move at the upper end of the box body, so that an opening at the upper end of the box body is controlled to be opened and closed; the high-light lamp can be adjusted horizontally and vertically according to actual requirements, so that sufficient illumination conditions can be provided for biological cells on the culture medium.

Description

Biological cell culture device with micro-vibration stress stimulation

Technical Field

The invention relates to the technical field of biological cell culture, in particular to a biological cell culture device with micro-vibration stress stimulation.

Background

With the continuous improvement of the manufacturing technology level, the scientific research on the microorganisms is supported by more powerful and hard conditions, and because of the needs of medical treatment and biological development, the research on the biological cells is in a state of high-speed development in recent years, and particularly, the research on the bone cells is advanced to a great extent. However, under the existing conditions, the technical short plate causes a barrier to the deep research of biological cells, and further research is influenced. Mechanical stress in vivo is a key inducing factor and plays a crucial role in the growth and repair of bones. Studies have shown that vibration, as one of the stress stimuli, has good effects of promoting bone formation and bone remodeling. The vibration stress is beneficial to the maturity and reconstruction of bones, and the vibration strength, the vibration frequency and the vibration amplitude influence the bone formation effect of vibration.

However, the specific biological action mechanism of micro-vibration is not completely clear at present, and the prior art lacks of related technical means, can realize stress stimulation on two-dimensional and three-dimensional systems in the process of cell culture, and can not detect the change of biochemical level of various intracellular molecules, although the micro-vibration instrument is adopted, the micro-vibration instrument is used as an independent device singly, and is combined by an excitation power amplifier, a communication module, a vibration signal adjusting controller, a vibration table and the like, but since the micro-vibration stimulation is usually very low amplitude, low intensity or low frequency vibration, and is also continuously or discontinuously performed for a long time, too many unstable factors exist in the culture of biological cells, so that the irregular interference to the biological cells is easily caused by the unstable structure of an internal box body of a common cell culture box, and the accuracy of the culture and research data of the biological cells is influenced.

In addition, the culture of the biological cells, which are the objects of the existing research, needs to be performed under aerobic and luminous conditions, but the natural environment is difficult to effectively regulate and control the aerobic condition, so that a plurality of uncertain factors exist in the process of culturing the biological cells, and the biological cells have great interference effect on the survival and the change of the biological cells.

Disclosure of Invention

The invention aims to solve the problems of stress stimulation and insufficient light oxygen in biological cell culture in the prior art, and provides a biological cell culture device with micro-vibration stress stimulation.

In order to achieve the purpose, the invention adopts the following technical scheme:

a biological cell culture device with micro-vibration stress stimulation comprises a box body with an opening at the upper end, wherein a cover body capable of being opened and closed is movably arranged at the opening at the upper end of the box body, and a stress mechanism and an illumination mechanism are respectively arranged in the box body and the cover body;

the stress mechanism comprises a first-stage support and a second-stage support which are fixedly arranged in a box body, a front shaft and a middle shaft are respectively arranged on the first-stage support, a limit bolt is arranged on the middle shaft, a first-stage motor connected with the middle shaft is fixedly arranged outside the box body, a ratchet wheel and a handle are respectively and fixedly connected with the front shaft and the middle shaft, a push rod corresponding to the ratchet wheel is connected to a pin shaft on the handle, a limit hole sleeved with the limit bolt in a sliding manner is arranged on the push rod, a movable latch corresponding to the ratchet wheel is arranged on the box body, a lifting frame is sleeved in the second-stage support in a sliding manner, a traction rod is connected between the movable latch and the lifting frame, a strong spring is fixedly sleeved on the lifting frame, a transmission shaft and a rotating shaft are respectively and rotatably arranged in the second-stage support, a first-stage gear and a second-stage, the lifting frame is fixedly provided with a bearing plate, the upper end of the bearing plate is respectively sleeved with a primary fixed magnetic block, a secondary fixed magnetic block and a movable magnetic block, the movable magnetic block is connected with a culture medium through a bolt, and cams positioned on two sides of the culture medium are fixedly sleeved on a rotating shaft;

the illumination mechanism comprises a second-stage motor fixedly mounted on the cover body, a vertical shaft connected with the output end of the second-stage motor is rotatably arranged on the outer side of the cover body, a fifth-stage gear is fixedly sleeved on the vertical shaft, a rack connected with the fifth-stage gear in a meshed mode is slidably arranged in the side wall of the cover body, a cover plate is fixedly connected onto the rack, two hydraulic cylinders are fixedly mounted at the upper end of the cover body and are connected with moving blocks together, a cylinder is fixedly connected to the lower end of each moving block, and a strong light is connected to the cylinder through bolts.

Preferably, the first-stage support and the second-stage support are horizontally distributed in the box body from bottom to top, the front shaft and the middle shaft are respectively rotatably sleeved at the front end and the middle end of the first-stage support, and the limiting bolt is fixedly connected to the rear end of the first-stage support.

Preferably, the front end of the push rod is movably abutted against the middle end of the ratchet wheel, the limiting hole is located at the rear end of the push rod, and the movable latch is movably abutted against the upper end of the ratchet wheel.

Preferably, the strong spring is positioned at the lower end of the lifting frame, and the upper end of the strong spring is welded with the secondary support.

Preferably, the first-stage gear and the second-stage gear are bevel gears, and the third-stage gear, the fourth-stage gear and the fifth-stage gear are flat gears.

Preferably, the bearing plate is connected to the upper end of the lifting frame above the secondary support, and the upper end of the bearing plate is provided with a guide groove for mounting the primary fixed magnetic block, the secondary fixed magnetic block and the movable magnetic block.

Preferably, the first-stage fixed magnetic block and the second-stage fixed magnetic block are respectively and fixedly installed at two ends of the guide groove, the movable magnetic block is slidably installed at the middle end of the guide groove, and like poles of the movable magnetic block, the first-stage fixed magnetic block and the second-stage fixed magnetic block are mutually repellent.

Preferably, the two hydraulic cylinders are horizontally arranged outside the upper end of the cover body, the air cylinders are vertically arranged in the lower end of the cover body, and the horizontal opening in the cover body is provided with a long hole in which the moving block is sleeved by a sliding sleeve.

Compared with the prior art, the invention has the following advantages:

1. the invention utilizes the primary bracket to sequentially arrange a front shaft provided with a ratchet wheel, a middle shaft provided with a handle and a limit bolt capable of limiting a push rod in a box body, and also arranges a movable latch which is movably propped against the ratchet wheel in the box body, and the secondary bracket is provided with a lifting frame which is elastically supported by a strong spring and is connected with the movable latch so as to provide continuous support capable of moving up and down for the bearing plate, thereby leading the culture medium on the bearing plate to be capable of vibrating stably in the vertical direction.

2. The invention uses the second-stage bracket to rotationally arrange the transmission shaft and the rotating shaft, and uses the first-stage gear and the second-stage gear which are connected in a meshed manner, the third-stage gear and the fourth-stage gear to drive the cam on the rotating shaft to rotate; the first-stage fixed magnetic block, the second-stage fixed magnetic block and the movable magnetic block with the same poles repelling each other are arranged in the bearing plate, and the movable magnetic block is used for providing a movable support capable of moving horizontally for the culture medium, so that the culture medium is subjected to stress stimulation in the horizontal moving process.

3. The invention arranges a vertical shaft driven by a second-stage motor to rotate outside the cover body, and utilizes a five-stage gear on the vertical shaft to drive a cover plate connected with a rack to horizontally move at the upper end of the box body, thereby controlling the opening and closing of the upper end opening of the box body and realizing the aerobic supply of a culture medium in the box body.

4. According to the invention, the cover body is internally provided with the moving block which is pulled by the hydraulic cylinder, and the moving block is provided with the cylinder which is connected with the floodlight, so that the floodlight can be horizontally and vertically adjusted according to actual requirements, and thus, sufficient illumination conditions can be provided for biological cells on a culture medium.

In conclusion, the invention is provided with the front shaft provided with the ratchet wheel, the middle shaft provided with the handle and the limit bolt which can limit the push rod, and the box body is internally provided with the movable latch which is movably propped against the ratchet wheel, and the ratchet wheel is urged to rotate by the node type to provide continuous support which can move up and down for the bearing plate, so that the culture medium can vibrate stably in the vertical direction; the first-stage fixed magnetic block, the second-stage fixed magnetic block and the movable magnetic block which have the same poles repelling each other are arranged in the bearing plate, and the movable magnetic block is used for providing a movable support which can move horizontally for the culture medium, so that the culture medium is subjected to stress stimulation in the horizontal moving process; a cover plate connected with the rack is driven by a five-stage gear on the vertical shaft to horizontally move at the upper end of the box body, so that an opening at the upper end of the box body is controlled to be opened and closed; the high-light lamp can be adjusted horizontally and vertically according to actual requirements, so that sufficient illumination conditions can be provided for biological cells on the culture medium.

Drawings

FIG. 1 is a schematic structural diagram of a biological cell culture device with micro-vibration stress stimulation according to the present invention;

FIG. 2 is an enlarged schematic view of part A of a biological cell culture apparatus with micro-vibration stress stimulation according to the present invention;

FIG. 3 is an enlarged schematic view of part B of a biological cell culture apparatus with micro-vibration stress stimulation according to the present invention;

FIG. 4 is an enlarged schematic view of part C of a biological cell culture apparatus with micro-vibration stress stimulation according to the present invention;

FIG. 5 is a schematic structural diagram of a case of a biological cell culture apparatus with micro-vibration stress stimulation according to the present invention;

FIG. 6 is a partial structural diagram of a stress mechanism of a biological cell culture device with micro-vibration stress stimulation according to the present invention;

FIG. 7 is a schematic diagram showing the distribution of the center shaft, the transmission shaft and the rotation shaft of the biological cell culture apparatus with micro-vibration stress stimulation according to the present invention.

In the figure: the automatic culture device comprises a box body 1, a cover body 2, a primary support 3, a secondary support 4, a front shaft 5, a middle shaft 6, a limiting bolt 7, a ratchet wheel 8, a handle 9, a push rod 10, a limiting hole 11, a movable latch 12, a lifting frame 13, a traction rod 14, a powerful spring 15, a transmission shaft 16, a rotating shaft 17, a primary gear 18, a secondary gear 19, a tertiary gear 20, a quaternary gear 21, a bearing plate 22, a primary fixed magnet 23, a secondary fixed magnet 24, a movable magnet 25, a culture medium 26, a cam 27, a secondary motor 28, a vertical shaft 29, a five-stage gear 30, a rack 31, a cover plate 32, a hydraulic cylinder 33, a movable block 34, a cylinder 35, a powerful light 36, a slot 37 and a.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-7, a biological cell culture device with micro-vibration stress stimulation comprises a box body 1 with an opening at the upper end, a cover body 2 which can be opened and closed is movably arranged at the opening at the upper end of the box body 1, as can be seen particularly with reference to fig. 5 of the specification, the box body 1 and the cover body 2 adopt a freely detachable connection mode so as to carry out specific operation on biological cells on a culture medium 26 in the box body 1, and a stress mechanism and an illumination mechanism are respectively arranged in the box body 1 and the cover body 2;

the stress mechanism comprises a first-stage bracket 3 and a second-stage bracket 4 which are fixedly arranged in a box body 1, the first-stage bracket 3 and the second-stage bracket 4 are arranged in two sides of the box body 1, a front shaft 5 is respectively arranged on the first-stage bracket 3, a limit bolt 7 is arranged on a middle shaft 6, a first-stage motor 38 connected with the middle shaft 6 is fixedly arranged outside the box body 1, the first-stage motor 38 adopts a servo motor with the product model of HC-KFS13B, the middle shaft 6 can continuously rotate under the driving action of the first-stage motor 38, a ratchet 8 and a handle 9 are respectively and fixedly connected on the front shaft 5 and the middle shaft 6, a push rod 10 corresponding to the ratchet 8 is connected on the handle 9 through a pin shaft, a limit hole 11 sleeved with the limit bolt 7 in a sliding manner is arranged on the push rod 10, a movable latch 12 corresponding to the ratchet 8 is arranged on the pin shaft in the box body 1, the push rod 10 is pulled by, thereby, the ratchet wheel 8 is driven, so that the ratchet wheel 8 can perform staged rotary motion, a lifting frame 13 is slidably sleeved in the secondary support 4, a traction rod 14 is connected between the movable latch 12 and the lifting frame 13, a strong spring 15 is fixedly sleeved on the lifting frame 13, the movable latch 12 deflects up and down, the lifting frame 13 is supported by the traction rod 14, so that the lifting frame 13 drives the bearing plate 22 and the culture medium 26 to move up and down, a transmission shaft 16 and a rotating shaft 17 are respectively rotatably installed in the secondary support 4, a primary gear 18 and a secondary gear 19 which are connected in a meshing manner are respectively fixedly sleeved on the middle shaft 6 and the transmission shaft 16, and a tertiary gear 20 and a quaternary gear 21 which are connected in a meshing manner are respectively fixedly sleeved on the transmission shaft 16 and the rotating shaft 17, as can be known by specifically referring to the attached figure 3 of the specification, the bearing plate 22 is fixedly arranged on the lifting frame, The culture medium is characterized by comprising a secondary fixed magnetic block 24 and a movable magnetic block 25, wherein the movable magnetic block 25 is mutually repelled by the primary fixed magnetic block 23 and the secondary fixed magnetic block 24 in the horizontal moving process, so that the movable magnetic block 25 can support the culture medium 26 to quickly restore the original position, the culture medium 26 is connected to the movable magnetic block 25 through a bolt, cams 27 located on two sides of the culture medium 26 are fixedly sleeved on a rotating shaft 17, and the cams 27 are in intermittent contact with the outer side wall of the culture medium 26 in the rotating process, so that the culture medium 26 is squeezed, and the culture medium 26 can vibrate in the horizontal direction;

the illumination mechanism comprises a secondary motor 28 fixedly arranged on the cover body 2, the secondary motor 28 adopts a servo motor with the product model of HC-SFS103B, the outer side of the cover body 2 is rotatably provided with a vertical shaft 29 connected with the output end of the secondary motor 28, the vertical shaft 29 is vertically arranged, a five-stage gear 30 is fixedly sleeved on the vertical shaft 29, a rack 31 meshed with the five-stage gear 30 is arranged in the side wall of the cover body 2 in a sliding manner, the rack 31 penetrates through the side wall of the cover body 2 in a sliding manner, a cover plate 32 is fixedly connected on the rack 31, the cover plate 32 can horizontally slide on the upper end of the box body 1 to control the opening and closing of the upper end opening of the box body 1, two hydraulic cylinders 33 are fixedly arranged at the upper end of the cover body 2, the hydraulic cylinders 33 are YGS70 x 30 x 520 to enable the high-intensity light 36 to horizontally move and adjust, the two hydraulic cylinders 33 are commonly connected with a moving block 34, the lower, so that the floodlights 36 move in the vertical direction, the floodlights 36 are connected to the cylinder 35 by bolts, and the floodlights 36 can be used for fully and sufficiently illuminating the biological cells on the culture medium 26.

Specifically, referring to fig. 1-2 of the specification, the first-stage support 3 and the second-stage support 4 are horizontally distributed in the box body 1 from bottom to top, the front shaft 5 and the middle shaft 6 are respectively rotatably sleeved at the front end and the middle end of the first-stage support 3, the limit bolt 7 is fixedly connected to the rear end of the first-stage support 3, and the limit bolt 7 can guide and limit the push rod 10 which moves back and forth in a telescopic manner.

The front end of the push rod 10 is movably abutted against the middle end of the ratchet 8, the limiting hole 11 is positioned at the rear end of the push rod 10, the movable latch 12 is movably abutted against the upper end of the ratchet 8, and the movable latch 12 can be lifted upwards once when the ratchet 8 is pushed to deflect once so as to support the lifting frame 13 to vertically vibrate once with the bearing plate 22.

The strong spring 15 is positioned at the lower end of the lifting frame 13, the upper end of the strong spring 15 is welded with the secondary support 4, the lifting frame 13 can reset automatically under the elastic action of the strong spring 15, and meanwhile, the up-and-down moving stability of the lifting frame 13 and the bearing plate 22 can be improved.

Referring to fig. 2 in the specification, the first-stage gear 18 and the second-stage gear 19 are bevel gears, and the third-stage gear 20, the fourth-stage gear 21 and the fifth-stage gear 30 are flat gears.

The bearing plate 22 is connected to the upper end of the crane 13 above the secondary support 4, and the upper end of the bearing plate 22 is provided with a guide groove for installing the primary fixed magnetic block 23, the secondary fixed magnetic block 24 and the movable magnetic block 25, and the culture medium 26 is pushed in the horizontal direction, so that the movable magnetic block 25 moves in the range of the guide groove, and the stability of the horizontal movement of the culture medium 26 is ensured.

The primary fixed magnetic block 23 and the secondary fixed magnetic block 24 are respectively fixedly installed at two end positions of the guide groove, the movable magnetic block 25 is slidably installed at the middle end position of the guide groove, like poles of the movable magnetic block 25 and the primary fixed magnetic block 23 and the secondary fixed magnetic block 24 are mutually repulsive, and as can be seen in specific reference to the attached figure 3 of the specification, the culture medium 26 can be ensured to perform reciprocating horizontal reverse vibration.

Two pneumatic cylinders 33 level sets up outside lid 2 upper end, and cylinder 35 sets up perpendicularly in the lid 2 lower extreme, and the level is equipped with the slot hole 37 that the movable block 34 was established to the slip cap in the lid 2, can guarantee the stationarity that highlight 36 removed in the horizontal direction.

The invention can be illustrated by the following operating modes:

under the condition that the two modified plates 32 horizontally abut against each other to seal the opening at the upper end of the box body 1, the first-stage motor 38 is controlled to be started outside the box body 1;

the output end of the primary motor 38 drives the middle shaft 6 to rotate on the primary support 3, and the middle shaft 6 drives the handle 9 to rotate so as to draw the push rod 10, so that the push rod 10 moves by taking the limit bolt 7 as one end for guiding;

the front end of the push rod 10 is abutted against the middle end of the ratchet 8 in the forward moving process, the ratchet 8 and the front shaft 5 are pushed to rotate for a certain angle on the primary support 3, and the ratchet 8 extrudes the movable latch 12 in the rotating process, so that the movable latch 12 takes the pin shaft mounting end as a fulcrum to lift the front end;

the movable latch 12 extrudes the lifting frame 13 upwards through the traction rod 14, the lifting frame 13 vertically moves upwards in the secondary support 4 and extrudes the strong spring 15, so that the lifting frame 13 moves up and down under the combined action of the elasticity of the strong spring 15 and the movable latch 12, and the bearing plate 22 supports the culture medium 26 to shake up and down;

the middle shaft 6 drives the transmission shaft 16 to rotate through a first-stage gear 18 and a second-stage gear 19 which are connected in a meshed mode, the transmission shaft 16 drives the rotating shaft 17 to rotate through a third-stage gear 20 and a fourth-stage gear 21 which are connected in a meshed mode, and the rotating shaft 17 drives the cam 27 to rotate so as to extrude the culture medium 26 from two side positions;

the stressed culture medium 26 enables the movable magnetic block 25 to slide left and right in the transverse groove on the bearing plate 22, and the homopolar repulsion of the movable magnetic block 25 and the primary fixed magnetic block 23 and the secondary fixed magnetic block 24 is facilitated, so that the culture medium 26 can reciprocate in the horizontal direction;

when illumination and oxygen are required to be provided for biological cells on the culture medium 26, the second-stage motor 28 is started, the output end of the second-stage motor 28 drives the vertical shaft 29 to rotate, the vertical shaft 29 drives the fifth-stage gear 30 to rotate, the fifth-stage gear 30 drives the rack 31 which is meshed and connected with the fifth-stage gear 30 to extend out of the cover body 2, so that the two cover plates 32 are reversely evacuated, and the upper end opening of the box body 1 is opened;

two hydraulic cylinders 33 are controlled to be opened and closed on the cover body 2, so that the moving block 34 horizontally moves in the long hole 37 and drives the air cylinder 35 to synchronously and horizontally move, then the air cylinder 35 is controlled to be opened, and the air cylinder 35 drives the highlight lamp 36 to move downwards so as to illuminate biological cells on the culture medium 26.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A biological cell culture device with micro-vibration stress stimulation comprises a box body (1) with an opening at the upper end, and is characterized in that the opening at the upper end of the box body (1) is movably provided with a cover body (2) which can be opened and closed, and a stress mechanism and an illumination mechanism are respectively arranged in the box body (1) and the cover body (2);

the stress mechanism comprises a first-stage support (3) and a second-stage support (4) which are fixedly arranged in a box body (1), a front shaft (5) and a limit bolt (7) are respectively arranged on the first-stage support (3), a first-stage motor (38) connected with the middle shaft (6) is fixedly arranged outside the box body (1), a ratchet wheel (8) and a handle (9) are respectively and fixedly connected onto the front shaft (5) and the middle shaft (6), a push rod (10) corresponding to the ratchet wheel (8) is connected onto the handle (9) through a pin shaft, a limit hole (11) of the limit bolt (7) is formed in the push rod (10) in a sliding sleeved mode, a movable latch (12) corresponding to the ratchet wheel (8) is arranged on the inner pin shaft of the box body (1), a lifting frame (13) is arranged in the second-stage support (4) in a sliding sleeved mode, and a traction rod (14) is connected between the movable latch (12), the culture medium lifting device is characterized in that a powerful spring (15) is fixedly sleeved on the lifting frame (13), a transmission shaft (16) and a rotating shaft (17) are respectively rotatably installed in the secondary support (4), a primary gear (18) and a secondary gear (19) which are connected in a meshed mode are respectively and fixedly sleeved on the middle shaft (6) and the transmission shaft (16), a tertiary gear (20) and a quaternary gear (21) which are connected in a meshed mode are respectively and fixedly sleeved on the transmission shaft (16) and the rotating shaft (17), a bearing plate (22) is fixedly arranged on the lifting frame (13), a primary fixed magnetic block (23), a secondary fixed magnetic block (24) and a movable magnetic block (25) are respectively sleeved on the upper end of the bearing plate (22), a culture medium (26) is connected on the movable magnetic block (25) through bolts, and cams (27) located on two sides of the culture medium (26;

the illumination mechanism comprises a secondary motor (28) fixedly mounted on a cover body (2), a vertical shaft (29) connected with the output end of the secondary motor (28) is rotatably arranged on the outer side of the cover body (2), a five-stage gear (30) is fixedly sleeved on the vertical shaft (29), a rack (31) meshed with the five-stage gear (30) is slidably arranged in the side wall of the cover body (2), a cover plate (32) is fixedly connected onto the rack (31), two hydraulic cylinders (33) are fixedly mounted at the upper end of the cover body (2), a moving block (34) is jointly connected onto the hydraulic cylinders (33), a cylinder (35) is fixedly connected onto the lower end of the moving block (34), and a strong light (36) is connected onto the cylinder (35) through bolts.

2. The biological cell culture device with the micro-vibration stress stimulation function as claimed in claim 1, wherein the primary support (3) and the secondary support (4) are horizontally distributed in the box body (1) from bottom to top, the front shaft (5) and the middle shaft (6) are respectively rotatably sleeved at the front end and the middle end of the primary support (3), and the limiting bolt (7) is fixedly connected to the rear end of the primary support (3).

3. The biological cell culture device with the micro-vibration stress stimulation function according to claim 1, wherein the front end of the push rod (10) movably supports against the middle end of the ratchet wheel (8), the limiting hole (11) is located at the rear end of the push rod (10), and the movable latch (12) movably supports against the upper end of the ratchet wheel (8).

4. The biological cell culture device with micro-vibration stress stimulation according to claim 2, characterized in that the strong spring (15) is positioned at the lower end of the lifting frame (13), and the upper end of the strong spring (15) is welded with the secondary bracket (4).

5. The device for culturing biological cells with micro-vibration stress stimulation according to claim 1, wherein the primary gear (18) and the secondary gear (19) are bevel gears, and the tertiary gear (20), the quaternary gear (21) and the quintuplet gear (30) are flat gears.

6. The biological cell culture device with micro-vibration stress stimulation as claimed in claim 4, wherein the bearing plate (22) is connected to the upper end of the lifting frame (13) above the secondary bracket (4), and the upper end of the bearing plate (22) is provided with a guide groove for mounting the primary fixed magnetic block (23), the secondary fixed magnetic block (24) and the moving magnetic block (25).

7. The device for culturing biological cells with micro-vibration stress stimulation according to claim 6, wherein the primary fixed magnetic block (23) and the secondary fixed magnetic block (24) are respectively fixedly arranged at two ends of the guide slot, the movable magnetic block (25) is slidably arranged at the middle end of the guide slot, and the same poles of the movable magnetic block (25) and the primary fixed magnetic block (23) and the secondary fixed magnetic block (24) are mutually repelled.

8. The device for culturing biological cells with micro-vibration stress stimulation according to claim 1, wherein the two hydraulic cylinders (33) are horizontally arranged outside the upper end of the cover body (2), the air cylinder (35) is vertically arranged in the lower end of the cover body (2), and the cover body (2) is horizontally provided with a long hole (37) which is sleeved with the moving block (34).