CN217418709U

CN217418709U - Cell culture case

Info

Publication number: CN217418709U
Application number: CN202220730799.XU
Authority: CN
Inventors: 杜万永; 尹金玉
Original assignee: Anhui Hesen Medical Laboratory Co ltd
Current assignee: Anhui Hesen Medical Laboratory Co ltd
Priority date: 2022-03-30
Filing date: 2022-03-30
Publication date: 2022-09-13
Anticipated expiration: 2032-03-30

Abstract

The utility model relates to a cell culture box, belonging to the biotechnology field, comprising a box body, wherein the top end inside the box body is provided with an LED lamp tube; the inside loading board that is provided with through elevating system of box, be provided with the square mouth of a plurality of on the loading board, square mouth one side is provided with the spout, the inside L shape pole that is provided with of spout, L shape pole one end extends to the inside and fixedly connected with movable block of square mouth, be provided with the spring between movable block and the square mouth, the tight piece of the first rubber of movable block one side fixedly connected with, the fixed block that is equipped with in square mouth one side, the tight piece of fixed block fixedly connected with second rubber clamp, adjust the distance between loading board and the LED fluorescent tube through the loading board up-and-down motion, control LED light source irradiation distance, mechanism setting through centre gripping cell culture bottle on the loading board, guarantee cell culture bottle's stability, provide suitable growing environment for the cell.

Description

Cell culture case

Technical Field

The utility model belongs to the technical field of it is biological, specifically, relate to a cell culture case.

Background

Light is an important natural factor affecting the biological characteristics of cells, and particularly, for photoreceptor cells, light rays with different wavelengths and different illumination intensities have different influences on the biological behaviors of the photoreceptor cells. In the present research aimed at in vitro of cells, the cell culture box with controllable light source is more rare, chinese patent CN204509353U discloses a cell culture box with controllable light source, which comprises a cell culture box body, an LED lamp panel is fixedly arranged on the upper portion in the cell culture box body, a cell culture dish placing plate capable of moving up and down for placing a cell culture dish is arranged on the lower portion in the cell culture box body, the continuous adjustment of illumination intensity is realized through the continuous adjustment of the distance between the LED lamp panel and the cell culture dish placing plate, but the culture box is only suitable for the condition of culturing cells by using the culture dish, which is not beneficial to the condition of culture by using a culture bottle, if the culture bottle is placed on the cell culture dish placing plate, on one hand, the culture bottle cannot be fixed to realize multi-directional light contact, so that the cell growth states at different positions in the cell culture bottle are different, on the other hand, in the process of adjusting the distance between the LED lamp panel and the cell culture dish placing plate, the culture flask is crooked, emptys easily, consequently, provides the controllable type cell culture case of light source that can be fixed to the cell culture flask centre gripping and is the technical problem that needs to solve at present.

SUMMERY OF THE UTILITY MODEL

For solving the technical problem existing in the prior art, the utility model provides a cell culture box.

The purpose of the utility model can be realized by the following technical scheme:

a cell culture box comprises a transport plate, universal wheels are fixedly mounted at four corners of the lower end of the transport plate, a box body is fixedly mounted at the upper end of the transport plate, a box door is movably hinged to the box body, an observation window made of toughened glass is mounted on the box door, the observation window is of a double-layer structure, and heating wires for heating the observation window are mounted on the inner side of the observation window;

a cavity and a T-shaped groove are formed in the side wall of one side of the box body, the cavity and the T-shaped groove are not communicated with each other, and a groove is formed in the side wall of the other side of the box body;

a bearing plate is arranged in the box body through a lifting mechanism, the two sides of the bearing plate are provided with raised rectangular plates, a plurality of square openings are formed in the bearing plate, a sliding groove is formed in one side of each square opening and is located at the top of the bearing plate, one side of the sliding groove is communicated with the corresponding square opening, an L-shaped rod is arranged in the sliding groove and is in clearance fit with the sliding groove, one end of the L-shaped rod penetrates through the sliding groove, the other end of the L-shaped rod penetrates through one side of the square opening and extends to the inside of the square opening, a movable block is fixedly connected to the inside of the square opening, a spring is arranged between the movable block and the square opening, the spring is sleeved on the L-shaped rod, a first rubber clamping block is fixedly connected to one side of the movable block, a fixed block is fixedly arranged on one side, away from the sliding groove, and a second rubber clamping block is fixedly connected to the fixed block;

furthermore, the lifting mechanism comprises a motor and a slide bar, the motor is positioned in the cavity, the output end of the motor is fixedly connected with a threaded rod through a coupler, one end of the threaded rod penetrates through the side wall of the cavity, extends into the T-shaped groove and is rotatably connected with the T-shaped groove, a rotary drum is connected to the threaded rod in a threaded manner, and a slide rail is fixedly connected to one side of the rotary drum; the sliding rod is fixedly arranged inside the groove, the sliding rod is connected with a sliding block in a sliding manner, and one side of the sliding block is fixedly connected with a sliding rail; the two sliding rails are matched with the bulges at the two sides of the bearing plate;

further, the bearing plate is internally provided with a through hole.

Further, the top end in the box body is provided with an LED lamp tube, and the bottom end in the box body is provided with a water tank.

The utility model has the advantages that:

the cell culture box body of the utility model is internally provided with the lifting mechanism and the bearing plate, the bearing plate is driven to move up and down by the lifting mechanism, the distance between the bearing plate and the LED lamp tube is adjusted by the up-and-down movement of the bearing plate, the LED light source wavelength and the illumination intensity are accurately controlled to create the appropriate growth condition for the cells in the culture bottle on the bearing plate, the mechanism for clamping the cell culture bottle is arranged on the bearing plate, the cell culture bottle is placed by clamping, compared with the existing flat lying placement, the contact area between the whole cell culture bottle and the light source is increased, the phenomenon that the cell culture bottle inclines and even collides when moving along with the lifting mechanism can be prevented, the problem that the cell culture bottle is unstable and can fall when being inserted and pulled away from the bearing plate can be prevented, therefore, the device not only has the characteristic of adjusting the light source intensity, but also has the function of stabilizing the culture bottle, has high practicability.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic view of the external structure of a cell incubator according to the present invention;

FIG. 2 is a schematic view of the internal structure of a cell incubator according to the present invention;

fig. 3 is a schematic structural diagram of the loading plate of the present invention.

In the drawings, the reference numbers indicate the following list of parts:

1. a transport plate; 11. a universal wheel; 2. a box body; 21. a cavity; 22. a T-shaped slot; 23. a groove; 3. an observation window; 4. a motor; 41. a threaded rod; 42. a rotating drum; 43. a slide rail; 5. a slide bar; 51. a slider; 6. a carrier plate; 61. a square opening; 62. a chute; 63. an L-shaped rod; 64. a movable block; 65. a first rubber clamping block; 66. a fixed block; 67. a second rubber clamping block; 68. a spring; 7. a water tank; 8. an LED lamp tube.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

Referring to fig. 1-3, a cell culture box comprises a transport plate 1, universal wheels 11 are fixedly mounted at four corners of the lower end of the transport plate 1, a box body 2 is fixedly mounted at the upper end of the transport plate 1, a box door is movably hinged on the box body 2, an observation window 3 made of toughened glass is mounted on the box door, the observation window 3 is of a double-layer structure, a heating wire for heating the observation window 3 is mounted on the inner side of the observation window 3, the observation window 3 can be heated, the temperature of the observation window 3 can be effectively increased, the observation window 3 is effectively prevented from generating condensate water, and the possibility of biological pollution caused by the condensate water of the observation window 3 is prevented;

an electronic device (not shown in the figure) for controlling the temperature, the humidity and the ratio of air and carbon dioxide is arranged in the box body 2, and the electronic device is a standard design for ion cell culture;

a cavity 21 and a T-shaped groove 22 are formed in the side wall of one side of the box body 2, the cavity 21 and the T-shaped groove 22 are not communicated with each other, and a groove 23 is formed in the side wall of the other side of the box body 2;

the box body 2 is internally provided with a bearing plate 6 through a lifting mechanism, the bearing plate 6 is a rectangular plate with two sides provided with protrusions, the bearing plate 6 is provided with a plurality of square openings 61, one side of each square opening 61 is provided with a sliding groove 62, the sliding groove 62 is positioned at the top of the bearing plate 6, one side of the sliding groove 62 is communicated with the corresponding square opening 61, the sliding groove 62 is internally provided with an L-shaped rod 63, the L-shaped rod 63 is in clearance fit with the sliding groove 62, one end of the L-shaped rod 63 penetrates through the sliding groove 62, the other end of the L-shaped rod 63 penetrates through one side of the square opening 61 and extends into the square opening 61, a movable block 64 is fixedly connected with the inside of the square opening 61, a spring 68 is arranged between the movable block 64 and the square opening 61, the spring 68 is sleeved on the L-shaped rod 63, one side of the movable block 64 is fixedly connected with a first rubber clamping block 65, one side of the square opening 61, which is far away from the sliding groove 62, and is fixedly provided with a fixed block 66, and fixedly connected with a second rubber clamping block 67, through the arrangement of the L-shaped rod 63, the movable block 64, the second rubber clamping block 67, the fixed block 66 and other mechanisms, the cell culture bottle can be firmly arranged on the bearing plate 6, and then the distance between the bearing plate 6 and the light source is adjusted, so that the optimal growth environment is provided for the cells;

the lifting mechanism comprises a motor 4 and a sliding rod 5, the motor 4 is positioned in the cavity 21, the output end of the motor 4 is fixedly connected with a threaded rod 41 through a coupler, one end of the threaded rod 41 penetrates through the side wall of the cavity 21, extends into the T-shaped groove 22 and is rotatably connected with the T-shaped groove 22, a rotary drum 42 is in threaded connection with the threaded rod 41, and a sliding rail 43 is fixedly connected to one side of the rotary drum 42; the sliding rod 5 is fixedly arranged in the groove 23, the sliding rod 5 is connected with a sliding block 51 in a sliding manner, and one side of the sliding block 51 is fixedly connected with a sliding rail 43; the two slide rails 43 are matched with the bulges at the two sides of the bearing plate 6; driven by the lifting mechanism, the bearing plate 6 can move up and down along the sliding rod 5;

the through holes are formed in the bearing plate 6, so that the flowing of light and air flow is facilitated, and the internal environment of the box body 2 is kept stable.

An LED lamp tube 8 is arranged at the top end inside the box body 2, and the wavelength and the illumination of an LED light source are accurately controlled to create proper growth conditions for cells in the culture bottle;

the bottom end in the box 2 is provided with a water tank 7 for maintaining the humidity inside the box 2 stable.

The working principle is as follows:

when the device is used, a cell bottle filled with cells to be cultured is placed on the bearing plate 6, the L-shaped rod 63 is pulled to one side away from the square opening 61, the L-shaped rod 63 drives the movable block 64 and the first rubber clamping block 65 to move, the spring 68 is compressed while the movable block 64 moves, then the cell culture bottle is placed between the first rubber clamping block 65 and the second rubber clamping block 67, the L-shaped rod 63 is slowly released, the elastic force of the spring 68 is released to push the first rubber clamping block 65 to clamp the cell culture bottle, the clamping mode is convenient and simple to operate, then the bearing plate 6 is placed between the two sliding rails 43, the motor 4 is started, the motor 4 drives the threaded rod 41 to rotate, the threaded rod 41 drives the rotary cylinder 42 to further drive the bearing plate 6 to move up and down, the distance between the cell culture bottle and the LED lamp tube 8 is adjusted through the up-down movement of the bearing plate 6, and the wavelength and the illumination of the LED light source are accurately controlled, creates proper growth conditions for the cells in the culture bottle.

In the description of the specification, reference to the description of "one embodiment," "an example," "a specific example," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing is merely exemplary and illustrative of the structure of the invention and various modifications, additions or substitutions as are known to those skilled in the art may be made to the described embodiments without departing from the scope of the invention as defined in the accompanying claims.

Claims

1. A cell culture box comprises a conveying plate (1), wherein a box body (2) is fixedly mounted at the upper end of the conveying plate (1), and an LED lamp tube (8) is arranged at the top end inside the box body (2), and is characterized in that a cavity (21) and a T-shaped groove (22) are formed in the side wall of one side of the box body (2), and a groove (23) is formed in the side wall of the other side of the box body (2);

the box (2) is inside to be provided with loading board (6) through elevating system, be provided with square mouthful of a plurality of (61) on loading board (6), square mouthful of (61) one side is provided with spout (62), spout (62) one side is linked together with square mouthful (61), spout (62) inside is provided with L shape pole (63), spout (62) are run through to L shape pole (63) one end, the other end extends to inside and fixedly connected with movable block (64) of square mouthful (61), be provided with spring (68) between movable block (64) and square mouthful (61), spring (68) cover is located on L shape pole (63), the first rubber of movable block (64) one side fixedly connected with presss from both sides tight piece (65), square mouthful (61) one side is fixed and is equipped with fixed block (66), fixed block (66) fixedly connected with second rubber presss from both sides tight piece (67).

2. The cell culture box according to claim 1, wherein the lifting mechanism comprises a motor (4) and a slide rod (5), the motor (4) is positioned inside the cavity (21), the output end of the motor (4) is fixedly connected with a threaded rod (41), the threaded rod (41) is rotatably connected with the T-shaped groove (22), the threaded rod (41) is in threaded connection with a rotary drum (42), and one side of the rotary drum (42) is fixedly connected with a slide rail (43).

3. A cell culture chamber according to claim 2, characterized in that the slide bar (5) is fixedly arranged inside the groove (23), a slide block (51) is slidably connected on the slide bar (5), and a slide rail (43) is fixedly connected on one side of the slide block (51).

4. A cell culture chamber according to claim 3, wherein the carrier plate (6) is a rectangular plate having protrusions formed on both sides thereof, and the slide rails (43) are engaged with the protrusions formed on both sides of the carrier plate (6).

5. A cell culture chamber according to claim 1, wherein the L-shaped bar (63) is a clearance fit with the slide groove (62).

6. A cell culture chamber according to claim 1, characterized in that the carrier plate (6) is provided with through holes in its interior.