CN107058099A - A kind of Embryo Culture and conveying arrangement - Google Patents

A kind of Embryo Culture and conveying arrangement Download PDF

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Publication number
CN107058099A
CN107058099A CN201710273934.6A CN201710273934A CN107058099A CN 107058099 A CN107058099 A CN 107058099A CN 201710273934 A CN201710273934 A CN 201710273934A CN 107058099 A CN107058099 A CN 107058099A
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conveying arrangement
culture
embryo
embryo culture
regulating system
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CN107058099B (en
Inventor
刁云飞
李晓霞
许保增
王士勇
张宇飞
常彤
李丹丽
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Institute Special Animal and Plant Sciences CAAS
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Institute Special Animal and Plant Sciences CAAS
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

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Abstract

The invention provides a kind of Embryo Culture and conveying arrangement, belong to cell transport field, this Embryo Culture and conveying arrangement, including incubator, supersonic generator, heat generating member, air passage one-way valve and for sense gas concentration lwevel, temperature and humidity inductor.Incubator includes culture chamber, and inductor is fixedly installed in culture chamber;Holding part is provided with the outside of one end of incubator, holding part includes system accommodating cavity and water tank, and regulating system is placed in system accommodating cavity;Embryo Culture and conveying arrangement also include regulating system, and regulating system is electrically connected with supersonic generator, and regulating system is electrically connected with heat generating member, and regulating system is connected with air passage one-way valve.By this Embryo Culture and conveying arrangement, temperature adjustment, humidity regulation and gas concentration lwevel regulation can be simultaneously provided in embryo or egg mother cell transportation, make the ecotopia that embryo or egg mother cell existence are reached in culture chamber.

Description

A kind of Embryo Culture and conveying arrangement
Technical field
The present invention relates to cell transport field, in particular to a kind of Embryo Culture and conveying arrangement.
Background technology
Embryo engineering technology is the research field quickly grown in recent years, not only to domestic animal genetic improvement, and open country is treasured in preservation Raw Animal resources play an important roll, and are also had a major impact in the infertile treatment of the mankind.However, due to animal-breeding base Often distant with laboratory, test specimen needs to transport go back to laboratory by the long period, and this frequently results in cell contamination, Reduce Cell viability.Simultaneously as cell is in transportation, in order to ensure the stability of transport, generally by test sample Product fixed placement, it is difficult to while meeting cell in the condition of culture needed for incubation.
Moreover, existing embryo or egg mother cell conveying arrangement can only all play insulation effect, it can not simulate well The ecotopia survived suitable for embryo.
The content of the invention
The invention provides a kind of Embryo Culture and conveying arrangement, it is intended to solves Embryo Culture in the prior art and transport dress Put the above mentioned problem of presence.
What the present invention was realized in:
A kind of Embryo Culture and conveying arrangement, including incubator, supersonic generator, heat generating member, air passage one-way valve and For sense gas concentration lwevel, temperature and humidity inductor.
The incubator includes culture chamber, and the inductor is fixedly installed in the culture chamber;
Holding part is provided with the outside of one end of the incubator, the holding part includes system accommodating cavity and water tank, described Regulating system is placed in the system accommodating cavity;
The Embryo Culture and conveying arrangement also include regulating system, the regulating system and supersonic generator electricity It is connected, the regulating system is electrically connected with the heat generating member, and the regulating system is connected with the air passage one-way valve.
In preferred embodiments of the present invention, the supersonic generator is arranged in the water tank, and the heat generating member is set Put the one side close to the holding part in the culture chamber.
In preferred embodiments of the present invention, the Embryo Culture and conveying arrangement also include pre- gas mixing device, described pre- Gas mixing device include premix gas holder and premix controlled atmosphere section Inductance valve, the gas outlet of the premix controlled atmosphere section Inductance valve with it is described The air intake connection of air passage one-way valve.
In preferred embodiments of the present invention, the Embryo Culture and conveying arrangement also include air flue, the air flue connection The water tank and culture chamber side wall, the gas outlet of the air passage one-way valve is connected with the air flue.
In preferred embodiments of the present invention, the Embryo Culture and conveying arrangement also include pallet, the culture chamber Interior one end close to the opening is provided with slideway, fix bar that the pallet includes and for placing straw or centrifuge tube Support body, the fix bar and the support body connected by flexible cord, and the fix bar can be fixed on the training by the slideway Support intracavitary.
In preferred embodiments of the present invention, the support body includes bottom and fixed bed, and the bottom is fixedly mounted on institute One side of the fixed bed away from the fix bar is stated, the bottom is provided with bracket.
In preferred embodiments of the present invention, the fixed bed is provided with the through hole coordinated with the bracket.
In preferred embodiments of the present invention, buffer bar is additionally provided with the culture chamber, the buffer bar includes first The body of rod and sealing are socketed in second body of rod in first body of rod, and described buffer bar one end is fixed with the culture cavity wall to be connected Connect, the other end abuts the support body.
In preferred embodiments of the present invention, the buffer bar is provided with multiple projections close to the side of the opening, many The individual projection is generally aligned in the same plane.
A kind of Embryo Culture and conveying arrangement, including handle, incubator, supersonic generator, heat generating member, air passage one-way valve And for sense gas concentration lwevel, temperature and humidity inductor.
The incubator includes culture chamber, and the inductor is fixedly installed in the culture chamber;
Holding part is provided with the outside of one end of the incubator, the holding part includes system accommodating cavity and water tank, described Regulating system is placed in the system accommodating cavity;
The Embryo Culture and conveying arrangement also include regulating system, the regulating system and supersonic generator electricity It is connected, the regulating system is electrically connected with the heat generating member, and the regulating system is connected with the air passage one-way valve;
The handle is arranged on the outside of the incubator.
The beneficial effects of the invention are as follows:The Embryo Culture and conveying arrangement provided by the present invention, can be in embryo or ovum Temperature adjustment, humidity regulation and gas concentration lwevel regulation are provided simultaneously in mother cell transportation, make to reach embryo in culture chamber Tire or the ecotopia of egg mother cell existence.Set by dismountable pallet and allow Embryo Culture and conveying arrangement root , in this state can be gentle by flexible cord according to the Embryo Culture being actually needed under selection installation and transportation frame progress travel position The effect of jumper bar, buffering is provided for support body, it is to avoid embryo damages in transportation.During without transporting, pallet is taken out, Plate support is replaced with, the static culture that plate carries out embryo is directly placed into.The device can be not only used for embryo's transport, exchange Section system can also carry out the transport of active seminal fluid after being adjusted.
Brief description of the drawings
In order to illustrate more clearly of the technical scheme of embodiment of the present invention, it will use below required in embodiment Accompanying drawing be briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore be not to be seen as It is the restriction to scope, for those of ordinary skill in the art, on the premise of not paying creative work, can be with root Other related accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the sectional view of the Embryo Culture that the embodiment of the present invention one is provided and conveying arrangement;
Fig. 2 is the section view under the Embryo Culture that the embodiment of the present invention one is provided and conveying arrangement placement centrifuge tube state Figure;
Fig. 3 is the structural representation of the Embryo Culture that the embodiment of the present invention one is provided and conveying arrangement;
Fig. 4 be in Fig. 1 II-II to sectional view;
Fig. 5 is the partial enlarged drawing of a-quadrant in Fig. 1;
Fig. 6 is the structural representation of plate support provided in an embodiment of the present invention;
Fig. 7 is the structural representation of the Embryo Culture that the embodiment of the present invention two is provided and conveying arrangement.
Icon:001- Embryo Cultures and conveying arrangement;010- incubators;020- supersonic generators;030- net with heating function; 040- air passage one-way valves;013- displays;100- culture chambers;200- inductors;300- holding parts;310- system accommodating cavities; 330- water tanks;311- regulating systems;011- extensions;331- air flues;410- premixes gas air bag;430- premix controlled atmosphere section sensings Valve;500- pallets;110- is open;111- slideways;510- fix bars;530- support bodys;550- flexible cords;570- centrifuge tubes; 531- bottoms;533- fixed beds;5331- through holes;5311- brackets;534- elastic pinching members;The fixture blocks of 5341- first;5343- second Fixture block;5345- fastening springs;590- buffer bars;The bodies of rod of 591- first;The bodies of rod of 593- second;580- projections;600- plate branch Frame;610- rack ventilating holes;050- handles.
Embodiment
To make the purpose, technical scheme and advantage of embodiment of the present invention clearer, below in conjunction with present invention implementation Accompanying drawing in mode, the technical scheme in embodiment of the present invention is clearly and completely described, it is clear that described reality The mode of applying is a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, ability The every other embodiment that domain those of ordinary skill is obtained under the premise of creative work is not made, belongs to the present invention The scope of protection.Therefore, the detailed description of embodiments of the present invention below to providing in the accompanying drawings, which is not intended to limit, wants The scope of the present invention of protection is sought, but is merely representative of the selected embodiment of the present invention.Based on the embodiment in the present invention, The every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, belongs to this Invent the scope of protection.
In the description of the invention, it is to be understood that the term of indicating position or position relationship is based on shown in accompanying drawing Orientation or position relationship, be for only for ease of the description present invention and simplify description, rather than indicate or imply signified equipment Or element there must be specific orientation, with specific azimuth configuration and operation, therefore it is not considered as limiting the invention.
In the present invention, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " fixation " etc. Term should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integrally;Can be that machinery connects Connect or electrically connect;Can be joined directly together, can also be indirectly connected to by intermediary, can be in two elements The connection in portion or the interaction relationship of two elements.For the ordinary skill in the art, can be according to specific feelings Condition understands the concrete meaning of above-mentioned term in the present invention.
In the present invention, unless otherwise clearly defined and limited, fisrt feature can be with or below second feature Directly contacted including the first and second features, can also include the first and second features not be direct contact but by them it Between other characterisation contact.Moreover, fisrt feature is on second feature, top and above to include fisrt feature special second Directly over levying and oblique upper, or fisrt feature level height is merely representative of higher than second feature.Fisrt feature second feature it Under, lower section and fisrt feature included below are immediately below second feature and obliquely downward, or are merely representative of fisrt feature level height Less than second feature.
Embodiment one
A kind of Embryo Culture and conveying arrangement 001 are present embodiments provided, referring to Fig. 1, this Embryo Culture and transport Device 001 includes incubator 010, supersonic generator 020, heat generating member and air passage one-way valve 040.
Include internal culture chamber 100 in incubator 010, inductor is installed with the side wall of culture chamber 100 200, inductor 200 can sense gas concentration lwevel, humidity and temperature in culture chamber 100.
The outer side bottom surface of incubator 010 is provided with holding part 300, holding part 300 and culture chamber 100 pass through incubator 010 bottom is mutually isolated.Holding part 300 includes system accommodating cavity 310 and water tank 330, is housed wherein in system holding part 300 There is regulating system 311, regulating system 311 is electrically connected with inductor 200, regulating system 311 can receive to transmit from inductor 200 Gas concentration lwevel signal, temperature signal and moisture signal, and analyzed and processed, and then corresponding is launched to corresponding equipment Adjustment signal.And be provided with the outside of incubator 010 gas concentration lwevel that display 013 is used to show in incubator 010, Temperature and humidity.The display 013 is touch-screen, can be by the virtual key on touch-screen to gas concentration lwevel, temperature Artificially adjusted with humidity.
Water tank 330 is arranged on the inner side away from inductor 200 of holding part 300, in incubator 010 away from inductor 200 Side be additionally provided with extension 011, extension 011 is hollow to form the connection culture chamber 100 of air flue 331 and water tank 330.Pass through gas Water in road 331, water tank 330 can enter in incubator 010, and the humidity in incubator 010 is adjusted.
Supersonic generator 020 is arranged in water tank 330, and supersonic generator 020 is electrically connected with regulating system 311, is led to Overregulate the switch for the adjustment Signal Regulation supersonic generator 020 that system 311 is sent.It is super that supersonic generator 020 is sent Sound wave can be atomized the water in water tank 330 so that the humidity that water smoke enters in the adjustment culture chamber 100 of culture chamber 100.
In the present embodiment, heat generating member is net with heating function 030, and net with heating function 030, which tiles, is arranged on the bottom of culture chamber 100, hair The current switch of heat supply network 030 is controlled by regulating system 311, to control the temperature in culture chamber 100.
Air passage one-way valve 040 is arranged on side of the extension 011 away from culture chamber 100, the outlet court of air passage one-way valve 040 Into air flue 331, the entrance of air passage one-way valve 040 is connected with pre- gas mixing device.Pre- gas mixing device include premix gas holder and Controlled atmosphere section Inductance valve 430 is premixed, it is premix gas air bag 410 that gas holder is premixed in the present embodiment, in premix gas air bag 410 It is adapted to the premix gas of gas concentration lwevel filled with embryonic development.Premix controlled atmosphere section Inductance valve 430 is arranged on premix gas air bag 410 It is interior, it can sense the gas concentration lwevel and premix gas air bag in premix gas air bag 410 by premixing controlled atmosphere section Inductance valve 430 Premix gas air pressure in 410, the controlling switch of air passage one-way valve 040 is connected with regulating system 311, can by regulating system 311 To control air passage one-way valve 040 to close or open, to control gas concentration lwevel in culture chamber 100.
Fig. 2, Fig. 3 and Fig. 4 are referred to, this Embryo Culture and conveying arrangement 001 also include pallet 500, in culture chamber 100 one end away from holding part 300 are provided with opening 110, and four slideways 111 towards bottom are provided with opening 110, fortune Defeated frame 500 includes fix bar 510 and support body 530, and fix bar 510 has two, and the two ends of fix bar 510 can pass through slideway 111 Slip into culture chamber 100 and abutted in the tip position of slideway 111.Pass through the phase of flexible cord 550 between fix bar 510 and support body 530 Even, to buffer the rigid collision of centrifuge tube 570 in transportation.
In the present invention, flexible cord 550 can wrap up in elastic component in addition or have what certain elastic material was made using rubber etc. Anti-flutter wire so that can be with bidirectional damping between fix bar 510 and support body 530 so that Embryo Culture and conveying arrangement 001 are above and below During vibration, with cushioning effect.
Support body 530 includes bottom 531 and fixed bed 533, and bottom 531 and fixed bed 533 are fixedly linked by connector, bottom Layer 531 is arranged on the one end of fixed bed 533 away from fix bar 510.Through hole 5331, centrifuge tube are provided with fixed bed 533 570 can be connected on bottom 531 by through hole 5331.The position corresponding with through hole 5331 is provided with support on bottom 531 Groove 5311, when centrifuge tube 570 is placed by through hole 5331 and is connected on bottom 531, the bottom of centrifuge tube 570 and support Groove 5311 coordinates so that the placement of centrifuge tube 570 is more stablized.
The elastic pinching member 534 being additionally provided with the both sides of through hole 5331, elastic pinching member 534 includes the first fixture block 5341, the Two fixture blocks 5343 and two fastening springs 5345, the first fixture block 5341 and the second fixture block 5343 are slidably disposed on through hole 5331 Both sides, when the first fixture block 5341 and the second fixture block 5343 are away from through hole 5331, through hole 5331 opens completely, two clampings Spring 5345 connects the first fixture block 5341 and the second fixture block 5343 allows the first fixture block 5341 and the second fixture block 5343 to pass through Elastic force is automatically close to each other.The stability of centrifuge tube 570 can not only be strengthened by elastic pinching member 534, while shortening the The distance of one fixture block 5341 and the second fixture block 5343 can clamp straw.Can be towards difference according to the needs of putting that straw is actual Direction be clipped between the first fixture block 5341 and the second fixture block 5343.And elastic pinching member 534 can be by setting on fixed bed 533 Put groove and recessiveness is located in fixed bed 533.
Fig. 1 and Fig. 5 are referred to, in the present embodiment, culture chamber 100 is rectangular cavity, is all provided with each side wall of rectangular cavity Three buffer bars 590 are equipped with, buffer bar 590 is set perpendicular to side wall, and buffer bar 590 includes first body of rod 591 and second body of rod 593, the sealing of the part of first body of rod 591 is set in second body of rod 593, and air cavity, first body of rod are internally formed in second body of rod 593 591 when pressing to second body of rod 593, can be by the air pressure formation cushioning effect of air cavity, and can pass through air cavity during distress resolves Interior air pressure returns to original position.In the present embodiment, the one end of second body of rod 593 away from first body of rod 591 is fixedly installed on side wall On, the one end of first body of rod 591 away from second body of rod 593 abuts the side of bottom 531.When support body 530 vibrates, it can pass through Row buffering is entered in vibration of the buffer bar 590 to support body 530.
Multiple projections 580 are additionally provided with the side wall of culture chamber 100, multiple projections 580 are arranged on same plane, projection 580 are arranged on the top of buffer bar 590, and the distance on the top of projection 580 to place side wall is than the top of buffer bar 590 to place side wall Distance it is short, this Embryo Culture and conveying arrangement 001 also include plate support 600 (referring to Fig. 6), and plate support 600 can It is used to place plate to be erected on projection 580, is provided with rack ventilating hole 610 uniformly dispersing on plate support 600.When not When needing pallet 500, placement plate can be abutted in projection embryo is cultivated.
In the present embodiment, culture chamber 100 can be not only rectangular cavity, or cylinder chamber, as long as passing through culture chamber 100 can place pallet 500, and buffer bar 590 not only can be arranged on side wall with second body of rod 593, and first body of rod 591 is supported Connect bottom 531, can also the first rod member be arranged on side wall, the second rod member abut bottom 531., rectangular cavity each side wall not Three buffer bars 590 can be only set, can also set four, five or other, the actual big of incubator 010 can be passed through Small, weight carries out appropriate adjustment.
The Embryo Culture and conveying arrangement 001 provided by the present invention, can be in embryo or egg mother cell transportation Temperature adjustment, humidity regulation and gas concentration lwevel regulation are provided simultaneously, make to reach embryo or egg mother cell life in culture chamber 100 The ecotopia deposited.Set by dismountable pallet 500 and allow Embryo Culture and conveying arrangement 001 according to actual need Select installation and transportation frame 500 to carry out the Embryo Culture under travel position, flexible cord 550 and buffering can be passed through in this state The effect of bar 590, is that support body 530 provides buffering, it is to avoid embryo damages in transportation.During without transporting, fortune is taken out Defeated frame 500, is directly placed into the static culture that plate carries out embryo.The device can be not only used for embryo's transport, to regulating system 311 be adjusted after can also carry out the transport of active seminal fluid.
Embodiment two
Present embodiments provide a kind of Embryo Culture and conveying arrangement 001, refer to Fig. 1 and Fig. 7, this Embryo Culture and Conveying arrangement 001 includes incubator 010, supersonic generator 020, heat generating member and air passage one-way valve 040.
Include internal culture chamber 100 in incubator 010, inductor is installed with the side wall of culture chamber 100 200, inductor 200 can sense gas concentration lwevel, humidity and temperature in culture chamber 100.
The outer side bottom surface of incubator 010 is provided with holding part 300, holding part 300 and culture chamber 100 pass through incubator 010 bottom is mutually isolated.Holding part 300 includes system accommodating cavity 310 and water tank 330, is housed wherein in system holding part 300 There is regulating system 311, regulating system 311 is electrically connected with inductor 200, regulating system 311 can receive to transmit from inductor 200 Gas concentration lwevel signal, temperature signal and moisture signal, and analyzed and processed, and then corresponding is launched to corresponding equipment Adjustment signal.
Water tank 330 is arranged on the inner side away from inductor 200 of holding part 300, in incubator 010 away from inductor 200 Side be additionally provided with extension 011, extension 011 is hollow and connection culture chamber 100 and the formation air flue 331 of water tank 330.Pass through Water in air flue 331, water tank 330 can enter in incubator 010, and the humidity in incubator 010 is adjusted.
Supersonic generator 020 is arranged in water tank 330, and supersonic generator 020 is electrically connected with regulating system 311, is led to Overregulate the switch for the adjustment Signal Regulation supersonic generator 020 that system 311 is sent.It is super that supersonic generator 020 is sent Sound wave can be atomized the water in water tank 330 so that the humidity that water smoke enters in the adjustment culture chamber 100 of culture chamber 100.
In the present embodiment, heat generating member is net with heating function 030, and net with heating function 030, which tiles, is arranged on the bottom and side of culture chamber 100 Wall, and set by metal cap cover to strengthen the uniformity of temperature, the current switch of net with heating function 030 is controlled by regulating system 311, To control the temperature in culture chamber 100.
Air passage one-way valve 040 is arranged on side of the extension 011 away from culture chamber 100, the outlet court of air passage one-way valve 040 Into air flue 331, the entrance of air passage one-way valve 040 is connected with pre- gas mixing device.In the present embodiment, pre- gas mixing device is premix Gas air bag 410, is adapted to the premix gas of gas concentration lwevel in premix gas air bag 410 filled with embryonic development.Air passage one-way valve 040 Controlling switch be connected with regulating system 311, by regulating system 311 air passage one-way valve 040 can be controlled to close or open, To control gas concentration lwevel in culture chamber 100.
Handle 050 is symmetrically arranged with the outside of incubator 010, Embryo Culture and conveying arrangement are facilitated by handle 050 001 carrying.
The Embryo Culture and conveying arrangement 001 provided by the present invention, can be in embryo or egg mother cell transportation Temperature adjustment, humidity regulation and gas concentration lwevel regulation are provided simultaneously, make to reach embryo or egg mother cell life in culture chamber 100 The ecotopia deposited.The device can be not only used for embryo's transport, and activity can also be carried out after being adjusted to regulating system 311 The transport of seminal fluid.
The preferred embodiment of the present invention is the foregoing is only, is not intended to limit the invention, for this area For technical staff, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made is any Modification, equivalent substitution, improvement etc., should be included in the scope of the protection.

Claims (10)

1. a kind of Embryo Culture and conveying arrangement, it is characterised in that including incubator, supersonic generator, heat generating member, gas circuit list To valve and for sense gas concentration lwevel, temperature and humidity inductor;
The incubator includes culture chamber, and the inductor is fixedly installed in the culture chamber;
Holding part is provided with the outside of one end of the incubator, the holding part includes system accommodating cavity and water tank, the regulation System is placed in the system accommodating cavity;
The Embryo Culture and conveying arrangement also include regulating system, the regulating system and the electric phase of the supersonic generator Even, the regulating system is electrically connected with the heat generating member, and the regulating system is connected with the air passage one-way valve.
2. Embryo Culture according to claim 1 and conveying arrangement, it is characterised in that the supersonic generator is arranged on In the water tank, the heat generating member is arranged on one side of the culture chamber close to the holding part.
3. Embryo Culture according to claim 1 and conveying arrangement, it is characterised in that the Embryo Culture and conveying arrangement Also include pre- gas mixing device, the pre- gas mixing device includes premix gas holder and premix controlled atmosphere section Inductance valve, the premix gas The gas outlet of regulation Inductance valve is connected with the air intake of the air passage one-way valve.
4. Embryo Culture according to claim 3 and conveying arrangement, it is characterised in that the Embryo Culture and conveying arrangement Also include air flue, the air flue connects the water tank and culture chamber side wall, the gas outlet of the air passage one-way valve with it is described Air flue is connected.
5. Embryo Culture according to claim 3 and conveying arrangement, it is characterised in that the Embryo Culture and conveying arrangement Also include one end in pallet, the culture chamber close to the opening and be provided with slideway, the fix bar that the pallet includes With the support body for placing straw or centrifuge tube, the fix bar and the support body are connected by flexible cord, and the fix bar can It is fixed on by the slideway in the culture chamber.
6. Embryo Culture according to claim 5 and conveying arrangement, it is characterised in that the support body includes bottom and fixation Layer, the bottom is fixedly mounted on one side of the fixed bed away from the fix bar, and the bottom is provided with bracket.
7. Embryo Culture according to claim 6 and conveying arrangement, it is characterised in that the fixed bed be provided with it is described The through hole that bracket coordinates.
8. Embryo Culture according to claim 5 and conveying arrangement, it is characterised in that be additionally provided with the culture chamber slow Jumper bar, the buffer bar includes first body of rod and seals second body of rod being socketed in first body of rod, the buffer bar one End is fixedly connected with the culture cavity wall, and the other end abuts the support body.
9. Embryo Culture according to claim 8 and conveying arrangement, it is characterised in that the buffer bar is close to the opening Side be provided with multiple projections, multiple projections are generally aligned in the same plane.
10. a kind of Embryo Culture and conveying arrangement, it is characterised in that including handle, incubator, supersonic generator, heat generating member, Air passage one-way valve and for sense gas concentration lwevel, temperature and humidity inductor;
The incubator includes culture chamber, and the inductor is fixedly installed in the culture chamber;
Holding part is provided with the outside of one end of the incubator, the holding part includes system accommodating cavity and water tank, the regulation System is placed in the system accommodating cavity;
The Embryo Culture and conveying arrangement also include regulating system, the regulating system and the electric phase of the supersonic generator Even, the regulating system is electrically connected with the heat generating member, and the regulating system is connected with the air passage one-way valve;
The handle is arranged on the outside of the incubator.
CN201710273934.6A 2017-04-24 2017-04-24 Embryo culture and transportation device Active CN107058099B (en)

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