CN113583872B - Embryo incubator - Google Patents
Embryo incubator Download PDFInfo
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- CN113583872B CN113583872B CN202110894616.8A CN202110894616A CN113583872B CN 113583872 B CN113583872 B CN 113583872B CN 202110894616 A CN202110894616 A CN 202110894616A CN 113583872 B CN113583872 B CN 113583872B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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Abstract
The invention relates to the technical field of embryo culture and discloses an embryo incubator, which comprises a case body, wherein the cross section of the inner cavity of the case body is circular, a vertical central shaft is rotatably arranged in the center of the case body, and a plurality of horizontally arranged support plates are distributed on the outer wall of the central shaft along a circumferential array; and a plurality of culture components are arranged on the top surface of each support plate. The culture assembly comprises a culture dish placing cylinder and a cylinder cover, wherein the two sides of the culture dish placing cylinder are respectively sunken with a placing cavity and a mounting cavity, the culture dish is fixed in the placing cavity, the cylinder cover is used for sealing the placing cavity, a detection module is mounted on the cylinder cover, and the detection module is used for detecting the internal environment of the placing cavity. An air inlet communicated with the placing cavity and the mounting cavity is formed in the shell of the culture dish placing cylinder, and an interlayer is further formed in the shell of the culture dish placing cylinder. The invention is convenient to monitor and regulate the internal environment of the embryo incubator, so that the embryo is cultivated in a proper environment all the time, and the invention is beneficial to the cultivation of high-quality embryos.
Description
Technical Field
The invention relates to the technical field of embryo culture, in particular to an embryo incubator.
Background
Embryo in vitro culture is a key element of auxiliary reproduction technology. The embryo incubator is a common tool for embryo culture, however, the common desktop or box incubator can only realize basic culture function, is inconvenient to monitor the internal environment of the incubator, is inconvenient to adjust basic environments such as temperature, humidity and mixed gas concentration, and is unfavorable for culturing high-quality embryos.
Disclosure of Invention
The present invention is directed to an embryo incubator for solving the above-mentioned problems of the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
an embryo incubator comprises a incubator body, wherein the cross section of the inner cavity of the incubator body is circular, a vertical central shaft is rotatably arranged in the center of the incubator body, and a plurality of horizontally arranged support plates are distributed on the outer wall of the central shaft along a circumferential array; and a plurality of culture components are arranged on the top surface of each support plate.
The culture assembly comprises a culture dish placing cylinder and a cylinder cover, wherein the two sides of the culture dish placing cylinder are respectively sunken with a placing cavity and a mounting cavity, the culture dish is fixed in the placing cavity, the cylinder cover is used for sealing the placing cavity, a detection module is mounted on the cylinder cover, and the detection module is used for detecting the internal environment of the placing cavity.
An air inlet communicated with the placing cavity and the mounting cavity is formed in the shell of the culture dish placing cylinder, and an interlayer is further formed in the shell of the culture dish placing cylinder.
The control assembly is arranged in the box body and used for controlling the internal environment of the placing cavity in each culture dish placing barrel.
Further, be provided with horizontal baffle in the box, control assembly locates the below of baffle, and extension board and culture assembly all are located the top of baffle.
Further, the control assembly comprises an installation box fixed in the box and a gas preparation assembly arranged in the installation box, a positioning frame for fixing the gas preparation assembly is further arranged in the installation box, heat preservation liquid is further stored in the installation box, a liquid pump is arranged outside the installation box, the input end of the liquid pump is communicated inside the installation box, the output end of the liquid pump is communicated with an interlayer in each shell through an infusion channel, a discharge pipe of the gas preparation assembly extends out of the box, and the output end of the gas preparation assembly is communicated with a placing cavity of each culture dish placing barrel through an air transmission channel.
Further, the top surface of extension board distributes has the location cassette that corresponds with the culture dish and places a section of thick bamboo, and the installation cavity inside intercommunication of a section of thick bamboo bottom is placed to the location cassette in with the culture dish, all is equipped with the hollow in the extension board, hollow inside and each location cassette inside intercommunication, and the inner wall of location cassette and the outer wall sealing connection of a section of thick bamboo is placed to the culture dish.
Further, the infusion channel comprises an infusion tube connected to the output end of the liquid pump, a liquid distribution tube fixed in the hollow cavity and an infusion channel arranged on the bottom surface of the culture dish placement barrel, infusion heads corresponding to the positioning clamping seats on the support plates are distributed on the liquid distribution tube, the bottom ports of the infusion channel are communicated with the tail ends of the corresponding infusion heads, and one ends of the liquid distribution tube extend out of the corresponding support plates and are connected with the infusion tube through a connecting mechanism.
Further, the connecting mechanism comprises a bearing table fixed on the inner wall of the box body, an annular temporary storage groove is formed in the bearing table, an annular sealing plate is coaxially connected to the annular notch of the temporary storage groove in a rotating mode, one end of the liquid distribution pipe, which extends out of the corresponding support plate, is communicated with a connector, the other end of the connector is connected with the annular sealing plate and is communicated with the interior of the temporary storage groove, and the tail end of the infusion pipe penetrates through the partition plate and is communicated with the temporary storage groove.
Further, the gas transmission channel comprises a cavity arranged in the central shaft, a first communication hole arranged on the central shaft shell, one end of the first communication hole is communicated with the cavity, the other end of the first communication hole is communicated with the hollow in the support plate, the hollow is communicated with the inside of the positioning clamping seat on the support plate, the inner wall of the positioning clamping seat is communicated with the mounting cavity of the corresponding culture dish placing barrel, the output end of the gas preparation assembly is connected with a transition mechanism on the outer side of the central shaft, and the other end of the transition mechanism is communicated with the cavity.
Further, the transition mechanism comprises an annular sleeve movably sleeved outside the central shaft and a gas pipe connected to the outer ring of the annular sleeve, a hollow is formed in the annular sleeve, an annular groove communicated with the hollow is formed in the inner ring of the annular sleeve, an annular plate is rotatably mounted at the annular groove, and air holes communicated with the hollow and the hollow are formed in the annular plate and the central shaft shell.
Further, the detection module comprises a gas concentration sensor, a temperature sensor and a plc controller, wherein the gas concentration sensor, the temperature sensor and a signal input end of the plc controller are electrically connected, and a signal output end of the plc controller is electrically connected with an electrical element in the control assembly.
The beneficial effects of the invention are as follows:
according to the embryo culture device provided by the invention, the embryo culture dishes are placed in the culture dish placing barrels, the corresponding barrel covers are closed, when the gas preparation assembly works, mixed gas is discharged, the mixed gas enters the hollow of each support plate through the gas transmission channel, the mixed gas in the hollow enters the clamping seat and finally enters the placing cavity through the gas inlet holes, so that the gas concentration in the placing cavity reaches a set value, the embryo storage environment is conveniently created, the heat generated by the working of the gas preparation assembly heats the liquid in the mounting box, the liquid is conveyed into the interlayer of each culture dish placing barrel through the liquid pump, the temperature in the culture dish placing barrel reaches the set value, the detection module is further arranged on the barrel covers of the culture dish placing barrels, and comprises the gas concentration sensor and the temperature sensor, so that the environment in the culture dish placing barrel is conveniently detected in real time, the real-time monitoring is convenient, and the success rate of embryo culture is ensured.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiments of the invention and together with the description serve to explain the invention and do not constitute a limitation on the invention. In the drawings:
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is an enlarged view of FIG. 1 at A in accordance with the present invention;
FIG. 3 is an enlarged view of FIG. 1 at B in accordance with the present invention;
FIG. 4 is an enlarged view of FIG. 2 at C in accordance with the present invention;
fig. 5 is an enlarged view of fig. 2D of the present invention.
In the figure: the box body 1, the flip cover 2, the discharge pipe 3, the positioning frame 4, the mounting box 5, the central shaft 6, the cavity 601, the shaft seat 7, the gas preparation assembly 8, the liquid pump 9, the infusion tube 10, the partition 11, the culture dish placing cylinder 12, the culture dish 13, the detection module 14, the cylinder cover 15, the gas transmission tube 16, the annular sleeve 17, the air hole 18, the hollow 19, the annular plate 20, the liquid separation tube 21, the support 22, the infusion head 23, the infusion channel 24, the positioning clamping seat 25, the air inlet 26, the interlayer 27, the baffle 28, the turntable 29, the adjusting motor 30, the connecting plate 31, the elastic telescopic mechanism 32, the bearing table 33, the connecting head 34, the annular sealing plate 35, the liquid separation tube 21 and the connecting plate 34,
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1:
referring to fig. 1-5, an embryo incubator comprises a case body 1, wherein the cross section of the inner cavity of the case body 1 is circular, a vertical central shaft 6 is rotatably arranged at the center of the inner cavity of the case body 1, and a plurality of horizontally arranged support plates 22 are distributed on the outer wall of the central shaft 6 along a circumferential array; a plurality of culturing components are arranged on the top surface of each supporting plate 22.
The culture assembly comprises a culture dish placing barrel 12 and a barrel cover 15, wherein placing cavities and mounting cavities are respectively recessed in two sides of the culture dish placing barrel 12, the culture dish 13 is fixed in the placing cavities, the barrel cover 15 is used for sealing the placing cavities, the barrel cover 15 is provided with a detection module 14, and the detection module 14 is used for detecting the internal environment of the placing cavities.
An air inlet hole 26 communicated with the placing cavity and the mounting cavity is formed in the shell of the culture dish placing cylinder 12, and an interlayer 27 is further formed in the shell of the culture dish placing cylinder 12.
A control component is arranged in the box body 1 and is used for controlling the internal environment of the placing cavity in each culture dish placing cylinder 12.
Referring to fig. 1, in order to make the apparatus more convenient to use, a horizontal partition 11 is disposed in the case 1, the control assembly is disposed below the partition 11, and the support plate 22 and the culture assembly are disposed above the partition 11.
Referring to fig. 1, the control assembly includes an installation box 5 fixed in the box 1 and a gas preparation assembly 8 disposed in the installation box 5, a positioning frame 4 for fixing the gas preparation assembly 8 is further disposed in the installation box 5, a heat preservation liquid is further stored in the installation box 5, a liquid pump 9 is installed outside the installation box 5, an input end of the liquid pump 9 is communicated with the inside of the installation box 5, an output end of the liquid pump 9 is communicated with an interlayer 27 in each shell through a transfusion channel, a discharge pipe 3 of the gas preparation assembly 8 extends out of the box 1, and an output end of the gas preparation assembly 8 is communicated with a placement cavity of each culture dish placement barrel 12 through a gas transmission channel.
Further, the top surface of the support plate 22 is provided with positioning clamping seats 25 corresponding to the culture dish placing cylinder 12, the positioning clamping seats 25 are internally communicated with the inside of the mounting cavity at the bottom of the culture dish placing cylinder 12, the support plate 22 is internally provided with a hollow part, the hollow part is internally communicated with the inside of each positioning clamping seat 25, and the inner wall of the positioning clamping seat 25 is in sealing connection with the outer wall of the culture dish placing cylinder 12.
Referring to fig. 2, further, the infusion channel includes an infusion tube 10 connected to an output end of the liquid pump 9, a liquid distribution tube 21 fixed in the hollow space, and an infusion channel 24 provided on a bottom surface of the culture dish placement barrel 12, wherein the liquid distribution tube 21 is distributed with infusion heads 23 corresponding to positioning holders 25 on the support plates 22, and a bottom port of the infusion channel 24 is communicated with a tail end of the corresponding infusion head 23, wherein one end of the liquid distribution tube 21 extends out of the corresponding support plate 22 and is connected with the infusion tube 10 through a connection mechanism. The heat preservation liquid can be conveyed into the interlayer of each culture dish placing cylinder through the mechanism.
Referring to fig. 4, in this solution, the connection mechanism includes a receiving table 33 fixed on the inner wall of the case 1, an annular temporary storage groove 36 is formed in the receiving table 33, an annular sealing plate 35 is coaxially rotatably connected to the annular groove opening of the temporary storage groove 36, one end of the liquid separating tube 21 extending out of the corresponding support plate 22 is communicated with a connector 34, the other end of the connector 34 is connected with the annular sealing plate 35 and is communicated with the interior of the temporary storage groove 36, and the end of the infusion tube 10 penetrates through the partition plate 11 and is communicated with the temporary storage groove 36.
Referring to fig. 5, the gas delivery channel includes a cavity 601 formed in the central shaft 6, a first communicating hole formed in a casing of the central shaft 6, one end of the first communicating hole is communicated with the cavity 601, the other end of the first communicating hole is communicated with a hollow in the support plate 22, the hollow is communicated with the inside of a positioning clamping seat 25 on the support plate 22, the inner wall of the positioning clamping seat 25 is communicated with a mounting cavity corresponding to the culture dish placing barrel 12, the output end of the gas preparation assembly 8 is connected with a transition mechanism outside the central shaft 6, and the other end of the transition mechanism is communicated with the cavity 601.
This scheme is still equipped with the shutoff in the installation cavity of each culture dish placement drum 12 and corresponds the shutoff mechanism of inlet port 26, wherein, place the cross section in chamber and be circular, the inlet port circumference is offered and is being placed the intracavity wall, the shutoff mechanism is including rotating the accommodate motor 30 of locating the intracavity bottom surface middle part of placing, accommodate motor 30's output shaft drive hookup has carousel 29, carousel 29 is cam structure, and place intracavity wall along its radial sliding connection has baffle 28, baffle 28 slip can shutoff corresponding inlet port 26, baffle 28's one end is contradicted with carousel 29's outer ring face, be fixed with connecting plate 31 on the baffle 28, connecting plate 31 is through elastic telescoping mechanism 32 and the inner wall connection who places the chamber, through accommodate motor 30 circular telegram work, drive carousel 29 is rotatory, drive baffle 28 slides, realize opening and closing of inlet port 26.
Referring to fig. 3, for facilitating rotation of the rotating shaft 6, a transition mechanism is designed to ensure that the gas transmission channel normally works to transmit mixed gas, the transition mechanism comprises an annular sleeve 17 movably sleeved on the outer side of the central shaft 6, and a gas transmission pipe 16 connected to the outer ring of the annular sleeve 17, a hollow 19 is formed in the annular sleeve 17, an annular groove communicated with the hollow 19 is formed in the inner ring of the annular sleeve 17, an annular plate 20 is rotatably mounted at the annular groove, and air holes 18 communicated with the hollow 19 and the hollow 601 are fixed on the annular plate 20 and the shell of the central shaft 6.
In this embodiment, the detection module 14 includes a gas concentration sensor, a temperature sensor, and a plc controller, where the gas concentration sensor, the temperature sensor, and a signal input terminal of the plc controller are electrically connected, and a signal output terminal of the plc controller is electrically connected to an electrical element in the control assembly. The gas concentration and the temperature value in the environment in the culture dish placing cylinder are detected by the gas concentration sensor and the temperature sensor, and when the gas concentration is lower than a set value, the plc controller controls the gas preparation assembly to work, continuously generates mixed gas and is intelligently controlled to the placing cavity of each culture dish placing cylinder through the gas transmission channel.
In this embodiment, the detection module 14 further includes a humidity sensor, and the humidity sensor is electrically connected to the plc controller.
When the temperature sensor detects that the temperature in the placing cavity is lower than the set value, the liquid pump works to convey the heat preservation liquid in the placing box 5 into the interlayer 27 through the infusion channel.
The gas preparation subassembly that this device put forward includes carbon dioxide preparation mechanism, and carbon dioxide preparation mechanism includes carbon dioxide generator and intensifiers, and carbon dioxide generator adopts the calcium bicarbonate heating preparation, and the chemical equation of its preparation is:
the temperature rising device is an electric heater to provide high temperature for the gas generator and can heat the heat preservation liquid.
The embryo culture device that this device provided, when using, place embryo culture dish in culture dish place a section of thick bamboo 12, then close corresponding cover 15, the work of gas preparation subassembly 8 that this device provided, emit the mixed gas, the mixed gas enters into the hollow of each extension board 22 through the gas-supply passageway, the mixed gas in the hollow enters into the cassette, and finally enter into through the inlet port and place the intracavity, make the mixed gas concentration of placing the intracavity reach the setting value, be convenient for embryo storage, mixed gas preparation subassembly work produces heat, liquid in the heating installation box 5, liquid is carried to each culture dish through liquid pump 9 and is placed the intermediate layer 27 of section of thick bamboo 12, make culture dish place a section of thick bamboo 12 inside temperature and reach the setting value, this device still installs detection module 14 on cover 15 of culture dish place a section of thick bamboo 12, detection module 14 includes gas concentration sensor and temperature sensor, the real-time detection culture dish place a section of thick bamboo 12 internal environment of thick bamboo is convenient for real-time monitoring, the success rate of embryo culture is guaranteed.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. An embryo incubator, characterized in that: the culture device comprises a box body (1), wherein the cross section of an inner cavity of the box body (1) is circular, a vertical central shaft (6) is rotatably arranged at the center of the inner cavity of the box body (1), a plurality of horizontally arranged support plates (22) are distributed on the outer wall of the central shaft (6) along a circumferential array, and a plurality of culture components are arranged on the top surface of each support plate (22);
the culture assembly comprises a culture dish placing barrel (12) and a barrel cover (15), wherein a placing cavity and an installing cavity are respectively recessed at two sides of the culture dish placing barrel (12), a culture dish (13) is fixed in the placing cavity, the barrel cover (15) is used for sealing the placing cavity, a detection module (14) is arranged on the barrel cover (15), and the detection module (14) is used for detecting the internal environment of the placing cavity;
an air inlet hole (26) communicated with the placing cavity and the mounting cavity is formed in the shell of the culture dish placing cylinder (12), and an interlayer (27) is also formed in the shell of the culture dish placing cylinder (12);
a control component is arranged in the box body (1) and is used for controlling the internal environment of the placing cavity in each culture dish placing cylinder (12);
a horizontal partition plate (11) is arranged in the box body (1), the control assembly is arranged below the partition plate (11), and the support plate (22) and the culture assembly are both arranged above the partition plate (11);
the control assembly comprises a mounting box (5) fixed in the box body (1) and a gas preparation assembly (8) arranged in the mounting box (5), a positioning frame (4) for fixing the gas preparation assembly (8) is further arranged in the mounting box (5), heat preservation liquid is further stored in the mounting box (5), a liquid pump (9) is arranged outside the mounting box (5), the input end of the liquid pump (9) is communicated with the inside of the mounting box (5), the output end of the liquid pump (9) is communicated with an interlayer (27) in each shell through a transfusion channel, a discharge pipe (3) of the gas preparation assembly (8) extends out of the box body (1), and the output end of the gas preparation assembly (8) is communicated with a placing cavity of each culture dish placing cylinder (12) through a gas transmission channel;
the top surface of the support plate (22) is provided with positioning clamping seats (25) corresponding to the culture dish placing barrels (12), the inside of the positioning clamping seats (25) is communicated with the inside of a mounting cavity at the bottom of the culture dish placing barrel (12), the support plate (22) is internally provided with a hollow, the hollow is communicated with the inside of each positioning clamping seat (25), and the inner wall of each positioning clamping seat (25) is connected with the outer wall of the culture dish placing barrel (12) in a sealing way;
the infusion channel comprises an infusion tube (10) connected to the output end of the liquid pump (9), a liquid distribution tube (21) fixed in the hollow space and an infusion channel (24) arranged on the bottom surface of the culture dish placement barrel (12), infusion heads (23) corresponding to positioning clamping seats (25) on the support plates (22) are distributed on the liquid distribution tube (21), bottom ports of the infusion channel (24) are communicated with the tail ends of the corresponding infusion heads (23), and one end of the liquid distribution tube (21) extends out of the corresponding support plates (22) and is connected with the infusion tube (10) through a connecting mechanism.
2. An embryo incubator as claimed in claim 1, wherein: the connecting mechanism comprises a bearing table (33) fixed on the inner wall of the box body (1), an annular temporary storage groove (36) is formed in the bearing table (33), an annular sealing plate (35) is coaxially connected to the annular notch of the temporary storage groove (36) in a rotating mode, one end of the liquid separating pipe (21) extending out of the corresponding support plate (22) is communicated with a connector (34), the other end of the connector (34) is connected with the annular sealing plate (35) and is communicated with the inside of the temporary storage groove (36), and the tail end of the infusion pipe (10) penetrates through the partition plate (11) and is communicated with the temporary storage groove (36).
3. An embryo incubator as claimed in claim 1 or claim 2, in which: the gas transmission channel comprises a cavity (601) formed in a central shaft (6), a first communication hole formed in a shell of the central shaft (6), one end of the first communication hole is communicated with the cavity (601), the other end of the first communication hole is communicated with a hollow in a support plate (22), the hollow is communicated with the inside of a positioning clamping seat (25) on the support plate (22), the inner wall of the positioning clamping seat (25) is communicated with a mounting cavity of a corresponding culture dish placing barrel (12), the output end of a gas preparation assembly (8) is connected with a transition mechanism on the outer side of the central shaft (6), and the other end of the transition mechanism is communicated with the cavity (601).
4. An embryo incubator as claimed in claim 3, in which: the transition mechanism comprises an annular sleeve (17) movably sleeved on the outer side of the central shaft (6), and a gas pipe (16) connected to the outer ring of the annular sleeve (17), wherein a hollow (19) is formed in the annular sleeve (17), an annular groove communicated with the hollow (19) is formed in the inner ring of the annular sleeve (17), an annular plate (20) is rotatably installed at the annular groove, and air holes (18) communicated with the hollow (19) and the cavity (601) are formed in the annular plate (20) and the shell of the central shaft (6).
5. An embryo incubator as claimed in claim 4, wherein: the detection module comprises a gas concentration sensor, a temperature sensor and a plc controller, wherein the gas concentration sensor, the temperature sensor and a signal input end of the plc controller are electrically connected, and a signal output end of the plc controller is electrically connected with an electrical element in the control assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110894616.8A CN113583872B (en) | 2021-08-05 | 2021-08-05 | Embryo incubator |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110894616.8A CN113583872B (en) | 2021-08-05 | 2021-08-05 | Embryo incubator |
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| Publication Number | Publication Date |
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| CN113583872A CN113583872A (en) | 2021-11-02 |
| CN113583872B true CN113583872B (en) | 2023-07-21 |
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| CN202110894616.8A Active CN113583872B (en) | 2021-08-05 | 2021-08-05 | Embryo incubator |
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| CN107058099A (en) * | 2017-04-24 | 2017-08-18 | 中国农业科学院特产研究所 | A kind of Embryo Culture and conveying arrangement |
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| CN110317730A (en) * | 2019-06-19 | 2019-10-11 | 禹州市龙跃牧业有限公司 | One kind is for the outer culture apparatus of bovine embryo |
| CN210765342U (en) * | 2019-07-26 | 2020-06-16 | 陶识博 | In-vitro embryo culture device |
| CN211814496U (en) * | 2020-02-15 | 2020-10-30 | 青岛农业大学 | Visual observation embryo development's device |
| CN211999752U (en) * | 2019-12-24 | 2020-11-24 | 青岛大学附属医院 | Test tube baby embryo incubator |
| CN112877214A (en) * | 2021-04-07 | 2021-06-01 | 中南大学湘雅医院 | Pressure controlled cell culture device for glaucoma research |
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| WO2018088245A1 (en) * | 2016-11-10 | 2018-05-17 | 株式会社片岡製作所 | Culture vessel housing apparatus |
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| JP2007267701A (en) * | 2006-03-31 | 2007-10-18 | Miyazaki Prefecture | Culture container, culture device and culture method |
| CN205275616U (en) * | 2015-12-12 | 2016-06-01 | 内蒙古草原生态畜牧产业研究院股份有限公司 | Carbon dioxide incubator |
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| CN110317730A (en) * | 2019-06-19 | 2019-10-11 | 禹州市龙跃牧业有限公司 | One kind is for the outer culture apparatus of bovine embryo |
| CN210765342U (en) * | 2019-07-26 | 2020-06-16 | 陶识博 | In-vitro embryo culture device |
| CN211999752U (en) * | 2019-12-24 | 2020-11-24 | 青岛大学附属医院 | Test tube baby embryo incubator |
| CN211814496U (en) * | 2020-02-15 | 2020-10-30 | 青岛农业大学 | Visual observation embryo development's device |
| CN112877214A (en) * | 2021-04-07 | 2021-06-01 | 中南大学湘雅医院 | Pressure controlled cell culture device for glaucoma research |
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| CN113583872A (en) | 2021-11-02 |
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