CN210765342U

CN210765342U - In-vitro embryo culture device

Info

Publication number: CN210765342U
Application number: CN201921199499.8U
Authority: CN
Inventors: 陶识博
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-07-26
Filing date: 2019-07-26
Publication date: 2020-06-16
Anticipated expiration: 2029-07-26

Abstract

The utility model discloses an external embryo culture device relates to external embryo culture technical field, a plurality of cultivation chambeies have been seted up in the box, cultivation chamber bottom is provided with the chamber of ventilating that is linked together with the cultivation chamber, ventilate the chamber and be connected with and be used for carrying CO₂The first delivery pipe and CO₂The air source is communicated, the first conveying pipe is provided with a one-way electromagnetic valve, and the culture cavity is internally provided with a culture cavityIs provided with CO₂The sensor, the sensor is connected with a controlling means with the one-way solenoid valve electricity, cultivate the intracavity be provided with cultivate chamber sliding connection place the drawer, it has a plurality of air vents to place drawer bottom equipartition, it is provided with the baffle that the size is greater than cultivates chamber opening size to place the drawer and be located the outer one end of box. The utility model provides an external embryo culture device can reduce the influence of external gas to other embryos in the box when getting the embryo put, improves the embryonic survival rate.

Description

In-vitro embryo culture device

Technical Field

The utility model belongs to the technical field of external embryo culture technique and specifically relates to an external embryo culture device is related to.

Background

The carbon dioxide incubator is a device for culturing cells/tissues in vitro by simulating and forming a growth environment similar to cells/tissues in organisms in an incubator body, wherein the incubator requires stable temperature (37 ℃), stable CO2 level (5%), constant pH value (pH value: 7.2-7.4) and higher relative saturation humidity (95%).

The existing culture device is not generally divided into zones, and when a plurality of embryos are cultured, different embryos are difficult to be divided and are easy to be mistaken. And chinese patent that patent application number is CN201720667670.8 discloses a precious animal oocyte and embryo culture device, the power distribution box comprises a box body, the top at the base is installed to the box, the top of interlayer is provided with adjustable division board, electric heating pipe and electric box all with control button electric connection, the utility model discloses at culture device internally mounted division board and interlayer, just so can cut apart the region of incubator inside, when cultivateing a plurality of cells and placenta, the condition of every cultivation just can be very convenient the clear.

However, the device still has some problems, in the actual culture process, a plurality of embryos are generally placed in the incubator to be cultured together, the box door needs to be opened when the embryos are observed and operated, and at the moment, gas in the incubator is exchanged with external gas, so that the gas composition and the temperature in the incubator are greatly changed, and the embryo survival rate is reduced; meanwhile, the outside air contains some harmful substances, and the embryos are damaged or even die when entering the incubator, so that the embryo culture effect is poor.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem, the utility model provides an external embryo culture device, it can reduce the influence that causes the box internal environment when opening the door operation, has improved embryonal survival rate.

The utility model discloses a realize above-mentioned purpose and specifically adopt following technical scheme:

an in vitro embryo culture device comprises a box body and a box door hinged with the box body, wherein a plurality of culture cavities are formed in the box body, and the bottoms of the culture cavities are provided with a culture plateThe culture cavity is communicated with a ventilation cavity, and the ventilation cavity is connected with a pipeline for conveying CO₂The first delivery pipe and CO₂The air source is communicated, the first conveying pipe is provided with a one-way electromagnetic valve, and the culture cavity is internally provided with CO₂The sensor, the sensor is connected with a controlling means with the one-way solenoid valve electricity, cultivate the intracavity be provided with cultivate chamber sliding connection place the drawer, it has a plurality of air vents to place drawer bottom equipartition, it is provided with the baffle that the size is greater than cultivates chamber opening size to place the drawer and be located the outer one end of box.

Furthermore, the box body is provided with a groove which is arranged on the outer side of the opening of the culture cavity, the baffle is provided with a locking block matched with the groove, and the locking block is sleeved with a first sealing ring.

Furthermore, a first gas channel transversely arranged is arranged below the ventilation cavity, a second gas channel communicated with the first gas channel is arranged at one end, away from the box door, of the box body, the second gas channel extends from the bottom of the box body to the top of the box body, and one end of the second gas channel penetrates through the box body and the CO₂The air source is communicated, and the first conveying pipe is communicated with the first air channel and the ventilation cavity.

Furthermore, first conveyer pipe one end extends to the intracavity of ventilating, it is provided with the chock plug with first conveyer pipe matched with to place drawer bottom, the chock plug setting is keeping away from baffle one end placing the drawer, it is provided with the slide rail to cultivate the chamber both sides, it is provided with the slider with slide rail matched with to place on the drawer.

Furthermore, a second sealing ring is arranged on the plug head.

The utility model has the advantages as follows:

the utility model provides an external embryo culture device is when using, and the culture dish that will be equipped with different embryos is placed in the drawer is placed to the difference, and when opening one of them and placing the drawer, the gas of this cultivation intracavity can produce the convection current with the outside air, and other cultivation chambeies are provided with the baffle owing to placing the drawer, and it can completely cut off the outside air, in the short time that the chamber door was opened, other embryos of cultivateing the intracavity can not receive the influence.

Drawings

In order to more clearly illustrate the technical solution of the present invention using the embodiments of the present invention, the drawings which are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic view of the overall structure of a culture apparatus according to the present invention;

FIG. 2 is a schematic view of the internal structure of the culture apparatus provided by the present invention;

reference numerals: 1-box body, 2-placing drawer, 3-box door, 4-ventilation cavity, 5-culture cavity, 6-first conveying pipe, 7-first gas channel, 8-plug, 9-baffle, 10-locking block, 11-ventilation hole, 12-second gas channel, 13-CO₂A sensor.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship that the products of the present invention are conventionally placed when used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed" and "in communication" are to be interpreted broadly, e.g., as either fixed or removable communication, or integrally connected; either mechanically or electrically; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Examples

Referring to fig. 1 and 2, the present embodiment provides an in vitro embryo culture apparatus, including a box body 1 and a box door 3 hinged to the box body 1, wherein a plurality of culture chambers 5 are formed in the box body 1, a ventilation chamber 4 communicated with the culture chambers 5 is formed at the bottom of the culture chambers 5, the ventilation chamber 4 is connected to a first delivery pipe 6 for delivering CO2, the first delivery pipe 6 is communicated with a CO2 gas source, wherein the CO2 gas source may be a gas cylinder or a CO2 generator or other device capable of providing carbon dioxide, the first delivery pipe 6 is provided with a one-way electromagnetic valve, the one-way electromagnetic valve is a common product on the market, and is an existing structure, which is not described herein again, a CO2 sensor 13 is disposed in the culture chamber 5, the sensor and the one-way electromagnetic valve are electrically connected to a control device, the CO2 sensor 13 is used for detecting the concentration of CO2 in the culture chamber, when the CO2 sensor 13 detects that the concentration of CO2 in the culture cavity 5 is lower than the preset value of the control device, the control device controls the one-way electromagnetic valve to be opened, the CO2 gas source enters the ventilation cavity 4 through the first delivery pipe 6 and then enters the culture cavity 5 to increase the concentration of CO2 in the culture cavity 5, when the concentration reaches the preset value of the control device, the control device controls the one-way electromagnetic valve to be closed, and the CO2 is stopped to be introduced into the culture cavity 5, wherein the control device is the existing equipment, is a known technology, and is not described again; in addition, a pressure relief pipe and a pressure detection device which are communicated with the culture cavity 5 can be arranged at the top of the culture cavity 5, a pressure relief groove is arranged at the top or the bottom of the box body 1, water is stored in the pressure relief groove, the upper end of the pressure relief groove is communicated with the outside, the outlet end of the pressure relief pipe is inserted from the top of the pressure relief groove and is positioned below the liquid level of the pressure relief groove, an electromagnetic valve is arranged on the pressure relief pipe, the electromagnetic valve is also arranged in a one-way mode, the pressure detection device and the electromagnetic valve are both electrically connected with a control device, when the pressure detection device detects that the pressure in the culture cavity 5 is overhigh, the control device controls the electromagnetic valve to be opened, and gas leaves the culture cavity 5 from the pressure; cultivate the drawer 2 of placing that is provided with and cultivates 5 sliding connection in the chamber 5, it has a plurality of air vents 11 to place 2 bottom equipartitions of drawer, it is provided with the baffle 9 that the size is greater than 5 opening sizes in cultivation chamber to place drawer 2 and lie in the outer one end of box 1, CO2 in the air vent 4 can get into more easily through air vent 11 and cultivates in the chamber 5, so that adjust the CO2 concentration of cultivateing in the chamber 5, and can set up second baffle 9 placing drawer 2 and baffle 9 relative one end, when taking out and placing drawer 2 in order to get the culture dish, second baffle 9 can be when cultivateing chamber 5 and open, play certain effect of blockking, reduce the convection current of cultivateing chamber 5 and outside air, be favorable to maintaining the CO2 concentration stability of cultivateing in the chamber 5. In addition, as with the existing incubator, a heat preservation device can be arranged in the box body 1, the heat preservation device can be an electric heating device, and can also be a heat preservation solution, when the electric heating device is the electric heating device, the electric heating pipes can be arranged at the top and the bottom of the culture cavity 5, namely, the electric heating pipes are positioned in the box body 1 partition above the culture cavity 5, at the two sides of the ventilation cavity 4 or in the box body 1 partition below the ventilation cavity 4, and the electric heating pipes positioned between the two culture cavities 5 are shared by 2 culture cavities 5; for the heat preservation liquid time, can set up the cavity that supplies the heat preservation liquid to flow at 5 tops of cultivateing the chamber and bottom, lie in two cavities of cultivateing 5 between promptly and cultivate 5 shares for two cultivation chambeies to make the cavity intercommunication in one end, make only need set up a inlet in 1 bottom of box, the top set up a liquid outlet can, this inlet and liquid outlet all link to each other with heating device, this heat preservation liquid can set up to the pure water.

Further, be provided with the recess around the 5 opening outsides in cultivation chamber on the box 1, be provided with on the baffle 9 with recess matched with locking piece 10, the cover is equipped with first sealing washer on the locking piece 10, when will placing drawer 2 and push into cultivation chamber 5, locking piece 10 card goes into in the recess, and first sealing washer can carry out better closure to cultivation chamber 5, prevents that external gas from getting into during embryo cultivation and cultivates chamber 5 to make the CO2 concentration of cultivateing in the chamber 5 more stable, improve the survival rate of embryo.

Furthermore, a first gas channel 7 is transversely arranged below the ventilation cavity 4, a second gas channel 12 communicated with the first gas channel 7 is arranged at one end, away from the box door 3, of the box body 1, the second gas channel 12 extends from the bottom of the box body 1 to the top of the box body 1, one end of the second gas channel 12 penetrates through the box body 1 to be communicated with a CO2 gas source, the first conveying pipe 6 is communicated with the first gas channel 7 and the ventilation cavity 4, in the actual use process, a pipeline for introducing gas is not required to be buried in the box body 1, and the gas is directly conveyed through the first gas channel 7 and the second gas channel 12, so that the introduction of the CO2 gas is more convenient, and the condition of gas leakage caused by the looseness of the pipeline connection position is avoided; in order to prevent the waste of CO2 gas, one end of the first conveying pipe 6 extends into the ventilation cavity 4, a plug 8 matched with the first conveying pipe 6 is arranged at the bottom of the placing drawer 2, the plug 8 is arranged at one end, far away from the baffle 9, of the placing drawer 2, slide rails are arranged on two sides of the culture cavity 5, a slide block matched with the slide rails is arranged on the placing drawer 2, when a culture dish is taken and placed, the placing drawer 2 is pulled out, the plug 8 moves along with the placing drawer 2, when the placing drawer 2 moves to the outermost side, the plug 8 is inserted into the first conveying pipe 6 to seal the first conveying pipe 6, the CO2 gas is prevented from leaving the first conveying pipe 6 to enter the culture cavity 5 to cause waste, a second sealing ring can be arranged on the plug 8, and the sealing effect between the plug 8 and the first conveying pipe 6 is improved; in addition, the vent hole 11 can be arranged between the plug 8 and the baffle 9, namely, the vent hole 11 is not arranged between the plug 8 and the second baffle 9, so that when the drawer 2 is pulled out and placed, the exchange of the outside air and the gas in the culture cavity 5 can be reduced.

The above description is only for the preferred embodiment of the present invention, and the present invention is not limited thereto, the protection scope of the present invention is defined by the claims, and all structural changes equivalent to the contents of the description and drawings of the present invention should be included in the protection scope of the present invention.

Claims

1. The in vitro embryo culture device comprises a box body (1) and a box door (3) hinged with the box body (1), and is characterized in that a plurality of culture cavities (5) are formed in the box body (1), a ventilation cavity (4) communicated with the culture cavities (5) is formed in the bottom of the culture cavity (5), the ventilation cavity (4) is connected with a first conveying pipe (6) used for conveying CO2, the first conveying pipe (6) is communicated with a CO2 air source, a one-way electromagnetic valve is arranged on the first conveying pipe (6), a CO2 sensor (13) is arranged in the culture cavity (5), the CO2 sensor (13) is electrically connected with the one-way electromagnetic valve to form a control device, a placing drawer (2) in sliding connection with the culture cavity (5) is arranged in the culture cavity (5), and a plurality of vent holes (11) are uniformly distributed at the bottom of the placing drawer (2), one end of the placing drawer (2) positioned outside the box body (1) is provided with a baffle (9) with the size larger than the opening size of the culture cavity (5).

2. The in vitro embryo culture device according to claim 1, wherein the box body (1) is provided with a groove around the outside of the opening of the culture chamber (5), the baffle (9) is provided with a locking block (10) matched with the groove, and the locking block (10) is sleeved with a first sealing ring.

3. The in vitro embryo culture device according to claim 1, wherein a first gas channel (7) is transversely arranged below the ventilation cavity (4), a second gas channel (12) communicated with the first gas channel (7) is arranged at one end of the box body (1) far away from the box door (3), the second gas channel (12) extends from the bottom of the box body (1) to the top of the box body (1), one end of the second gas channel (12) passes through the box body (1) and is communicated with a CO2 gas source, and the first conveying pipe (6) is communicated with the first gas channel (7) and the ventilation cavity (4).

4. The in vitro embryo culture device according to claim 3, wherein one end of the first delivery pipe (6) extends into the ventilation cavity (4), the bottom of the placing drawer (2) is provided with a plug (8) matched with the first delivery pipe (6), the plug (8) is arranged at one end of the placing drawer (2) far away from the baffle (9), two sides of the culture cavity (5) are provided with slide rails, and the placing drawer (2) is provided with slide blocks matched with the slide rails.

5. The in vitro embryo culture device according to claim 4, wherein the plug (8) is provided with a second sealing ring.