CN113583872A - Embryo incubator - Google Patents
Embryo incubator Download PDFInfo
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- CN113583872A CN113583872A CN202110894616.8A CN202110894616A CN113583872A CN 113583872 A CN113583872 A CN 113583872A CN 202110894616 A CN202110894616 A CN 202110894616A CN 113583872 A CN113583872 A CN 113583872A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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Abstract
The invention relates to the technical field of embryo culture and discloses an embryo incubator, which comprises a box body, wherein the cross section of an inner cavity of the box body is circular, a vertical central shaft is rotatably arranged at the center in the box body, and a plurality of support plates which are horizontally arranged are distributed on the outer wall of the central shaft along the circumferential direction in an array manner; the top surface of each support plate is provided with a plurality of culture components. The culture assembly comprises a culture dish placing barrel and a barrel cover, wherein the two sides of the culture dish placing barrel are respectively sunken with a placing cavity and an installing cavity, the culture dish is fixedly arranged in the placing cavity, the barrel cover is used for sealing the placing cavity, a detection module is installed on the barrel cover, and the detection module is used for detecting and placing the environment in the placing cavity. The casing of the culture dish placing barrel is internally provided with an air inlet hole communicated with the placing cavity and the mounting cavity, and the casing of the culture dish placing barrel is internally provided with an interlayer. The invention is convenient to monitor and adjust the internal environment of the embryo incubator, so that the embryo is always cultured in a proper environment, and the culture of high-quality embryos is facilitated.
Description
Technical Field
The invention relates to the technical field of embryo culture, in particular to an embryo incubator.
Background
Embryo in vitro culture is a key link of the assisted reproduction technology. Embryo incubator is embryo culture's instrument commonly used, however, general desktop formula or box incubator can only realize essential cultivation function, and inconvenient inside environment to the incubator monitors, also is not convenient for adjust basic environment such as temperature, humidity and mist concentration, is unfavorable for the cultivation of high quality embryo.
Disclosure of Invention
The invention aims to provide an embryo incubator to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
an embryo incubator comprises a box body, wherein the cross section of an inner cavity of the box body is circular, a vertical central shaft is rotatably arranged at the center in the box body, and a plurality of support plates which are horizontally arranged are distributed on the outer wall of the central shaft along the circumferential direction in an array manner; the top surface of each support plate is provided with a plurality of culture components.
The culture assembly comprises a culture dish placing barrel and a barrel cover, wherein the two sides of the culture dish placing barrel are respectively sunken with a placing cavity and an installing cavity, the culture dish is fixedly arranged in the placing cavity, the barrel cover is used for sealing the placing cavity, a detection module is installed on the barrel cover, and the detection module is used for detecting and placing the environment in the placing cavity.
The casing of the culture dish placing barrel is internally provided with an air inlet hole communicated with the placing cavity and the mounting cavity, and the casing of the culture dish placing barrel is internally provided with an interlayer.
The internal control assembly that is provided with of box is used for controlling each culture dish and places the internal environment of placing the chamber in the section of thick bamboo.
Furthermore, a horizontal partition plate is arranged in the box body, the control assembly is arranged below the partition plate, and the support plate and the culture assembly are both positioned above the partition plate.
Further, the control assembly still is equipped with the locating rack of fixed gaseous preparation subassembly including fixing the mounting box in the box and locating the gaseous preparation subassembly in the mounting box, heat preservation liquid has still been stored in the mounting box, and the externally mounted of mounting box has the liquid pump, and the input of liquid pump communicates inside the mounting box, and the output of liquid pump passes through the intermediate layer that the infusion passageway communicates in each casing, and the box is stretched out to the discharge pipe of gaseous preparation subassembly, and the output of gaseous preparation subassembly communicates through the gas transmission passageway and places the chamber of placing of a section of thick bamboo in each culture dish.
Furthermore, the top surface distribution of extension board has the location cassette of placing a section of thick bamboo with the culture dish and corresponding, and the inside intercommunication of installation cavity of bobbin base portion is placed with the culture dish in the location cassette, all is equipped with hollowly in the extension board, and hollow inside and each location cassette are inside to be communicated, and the outer wall sealing connection of a section of thick bamboo is placed to the inner wall of location cassette and culture dish.
Furthermore, the infusion passageway is including connecting the transfer line at the liquid pump output end, fixing branch liquid pipe in the hollow and set up the infusion way of placing a bottom surface at the culture dish, divide and distribute on the liquid pipe and have the infusion head that corresponds with cassette on this extension board, the bottom port that the infusion said communicates with the end that corresponds the infusion head, and wherein, the one end that divides the liquid pipe stretches out and corresponds the extension board and pass through coupling mechanism with the transfer line and connect.
Further, coupling mechanism is including fixing the accepting table at the box inner wall, has seted up annular groove of keeping in the accepting table, and the ring channel department coaxial rotation in groove of keeping in is connected with annular sealing plate, and the one end intercommunication that the branch liquid pipe stretches out corresponding extension board has the connector, the other end and the annular sealing plate of connector connect and with the inside intercommunication in groove of keeping in, the end of transfer line runs through the baffle, and link to each other in the groove of keeping in.
Furthermore, the gas transmission channel comprises a cavity arranged in the central shaft and a first communication hole arranged on the shell of the central shaft, one end of the first communication hole is communicated with the cavity, the other end of the first communication hole is communicated with the hollow part in the support plate, the hollow part is communicated with the interior of the clamping seat on the support plate, the inner wall of the clamping seat is communicated with the installation cavity corresponding to the culture dish placing cylinder, the output end of the gas preparation assembly is connected with a transition mechanism on the outer side of the central shaft, and the other end of the transition mechanism is communicated with the cavity.
Further, the transition mechanism comprises an annular sleeve and a gas pipe, wherein the annular sleeve is movably sleeved on the outer side of the central shaft, the gas pipe is connected to the outer ring of the annular sleeve, a hollow part is formed in the annular sleeve, an annular groove communicated with the hollow part is formed in the inner ring of the annular sleeve, an annular plate is rotatably installed at the annular groove, and gas holes communicated with the hollow part and the cavity are fixed on the annular plate and the central shaft shell.
Further, the detection module includes a gas concentration sensor, a temperature sensor and a plc controller, wherein the gas concentration sensor, the temperature sensor and a signal input terminal of the plc controller are electrically connected, and a signal output terminal of the plc controller is electrically connected to an electrical component in the control assembly.
The invention has the beneficial effects that:
the invention provides an embryo culture device, wherein an embryo culture dish is placed in a culture dish placing barrel, a corresponding barrel cover is closed, when a gas preparation component works, mixed gas is discharged, the mixed gas enters the hollow part of each support plate through a gas transmission channel, the mixed gas in the hollow part enters a clamping seat and finally enters a placing cavity through a gas inlet hole, so that the concentration of the gas in the placing cavity reaches a set value, an embryo storage environment is conveniently created, the heat generated by the work of the gas preparation component heats the liquid in an installation box, the liquid is conveyed to the inner part of an interlayer of each culture dish placing barrel through a liquid pump, the temperature in the culture dish placing barrel reaches the set value, a detection module is also installed on the barrel cover of the culture dish placing barrel, the detection module comprises a gas concentration sensor and a temperature sensor, and is convenient for detecting the environment in the culture dish placing barrel in real time, the real-time monitoring is convenient, and the success rate of embryo culture is ensured.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is an enlarged view taken at A of FIG. 1 in accordance with the present invention;
FIG. 3 is an enlarged view of FIG. 1 at B of the present invention;
FIG. 4 is an enlarged view of the invention at C of FIG. 2;
fig. 5 is an enlarged view of fig. 2D of the present invention.
In the figure: the device comprises a box body 1, a turnover cover 2, a discharge pipe 3, a positioning frame 4, a mounting box 5, a central shaft 6, a cavity 601, a shaft seat 7, a gas preparation component 8, a liquid pump 9, a liquid conveying pipe 10, a partition plate 11, a culture dish placing barrel 12, a culture dish 13, a detection module 14, a barrel cover 15, a gas conveying pipe 16, an annular sleeve 17, a gas hole 18, a hollow part 19, an annular plate 20, a liquid separating pipe 21, a support plate 22, a liquid conveying head 23, a liquid conveying channel 24, a positioning clamping seat 25, a gas inlet hole 26, an interlayer 27, a baffle plate 28, a rotary table 29, an adjusting motor 30, a connecting plate 31, an elastic telescopic mechanism 32, a bearing table 33, a connector 34, an annular sealing plate 35, a liquid conveying pipe, a positioning clamping seat 25, an air inlet 26, an interlayer 27, a baffle plate 28, a rotary table 29, an adjusting motor 30, a connecting plate 31, an elastic telescopic mechanism 32, a bearing table 33, a connecting plate 34, an annular sealing plate 35, a sealing plate, a sealing structure and a sealing structure, and a sealing structure, and a sealing structure,
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
referring to fig. 1-5, an embryo incubator comprises an incubator body 1, wherein the cross section of the inner cavity of the incubator body 1 is circular, a vertical central shaft 6 is rotatably mounted at the inner center of the incubator body 1, and a plurality of support plates 22 which are horizontally arranged are distributed on the outer wall of the central shaft 6 along the circumferential direction in an array manner; each plate 22 has a plurality of culture modules mounted to its top surface.
The culture assembly comprises a culture dish placing cylinder 12 and a cylinder cover 15, wherein the two sides of the culture dish placing cylinder 12 are respectively sunken with a placing cavity and an installing cavity, the placing cavity is internally fixed with a culture dish 13, the cylinder cover 15 is used for hermetically placing the cavity, a detection module 14 is installed on the cylinder cover 15, and the detection module 14 is used for detecting and placing the internal environment of the cavity.
An air inlet 26 communicated with the placing cavity and the mounting cavity is formed in the shell of the culture dish placing cylinder 12, and an interlayer 27 is further formed in the shell of the culture dish placing cylinder 12.
A control component is arranged in the box body 1 and used for controlling the internal environment of the placing cavity in each culture dish placing cylinder 12.
Referring to fig. 1, in order to make the device more convenient to use, a horizontal partition plate 11 is arranged in the box body 1, the control component is arranged below the partition plate 11, and the support plate 22 and the culture component are both arranged above the partition plate 11.
Referring to fig. 1, the control assembly includes a mounting box 5 fixed in the box body 1 and a gas preparation assembly 8 disposed in the mounting box 5, a positioning frame 4 for fixing the gas preparation assembly 8 is further disposed in the mounting box 5, a heat preservation liquid is further stored in the mounting box 5, a liquid pump 9 is mounted outside the mounting box 5, an input end of the liquid pump 9 is communicated with the inside of the mounting box 5, an output end of the liquid pump 9 is communicated with the interlayers 27 in the respective shells through a liquid conveying channel, a discharge pipe 3 of the gas preparation assembly 8 extends out of the box body 1, and an output end of the gas preparation assembly 8 is communicated with the placing cavities of the respective culture dish placing cylinders 12 through a liquid conveying channel.
Further, the top surface distribution of extension board 22 has the location cassette 25 of placing a section of thick bamboo 12 with the culture dish and corresponding, and the inside intercommunication of installation cavity of a section of thick bamboo 12 bottom is placed with the culture dish in the location cassette 25, all is equipped with hollowly in the extension board 22, and hollow inside and the inside intercommunication of each location cassette 25, and the outer wall sealing connection of a section of thick bamboo 12 is placed to the inner wall of location cassette 25 and culture dish.
Referring to fig. 2, further, the infusion path includes an infusion tube 10 connected to an output end of the liquid pump 9, a liquid distribution tube 21 fixed in the hollow, and an infusion path 24 formed on a bottom surface of the dish placing cylinder 12, an infusion head 23 corresponding to the clamping seat 25 on the support plate 22 is distributed on the liquid distribution tube 21, a bottom port of the infusion path 24 is communicated with a terminal corresponding to the infusion head 23, wherein one end of the liquid distribution tube 21 extends out of the corresponding support plate 22 and is connected to the infusion tube 10 through a connection mechanism. Through the mechanism, heat preservation liquid can be conveyed into the interlayers of the culture dish placing cylinders.
Referring to fig. 4, in the present embodiment, the connection mechanism includes a receiving platform 33 fixed on the inner wall of the box body 1, an annular temporary storage groove 36 is formed in the receiving platform 33, an annular sealing plate 35 is coaxially and rotatably connected to an annular groove opening of the temporary storage groove 36, one end of the liquid distribution pipe 21 extending out of the corresponding support plate 22 is communicated with a connector 34, the other end of the connector 34 is connected with the annular sealing plate 35 and is communicated with the inside of the temporary storage groove 36, and the tail end of the infusion pipe 10 penetrates through the partition plate 11 and is communicated with the temporary storage groove 36.
Referring to fig. 5, the gas transmission channel includes a cavity 601 formed in the central shaft 6, a first communication hole formed in the shell of the central shaft 6, one end of the first communication hole is communicated with the cavity 601, the other end of the first communication hole is communicated with the hollow in the support plate 22, the hollow is communicated with the inside of the clamping seat 25 on the support plate 22, the inner wall of the clamping seat is communicated with the installation cavity corresponding to the culture dish placing cylinder 12, the output end of the gas preparation assembly 8 is connected with the transition mechanism outside the central shaft 6, and the other end of the transition mechanism is communicated with the cavity 601.
This scheme is in, each culture dish is placed the installation intracavity of a section of thick bamboo 12 and is still equipped with the shutoff mechanism that the shutoff corresponds inlet port 26, wherein, the cross section of placing the chamber is circular, the inlet port circumference is seted up and is being placed the intracavity wall, shutoff mechanism locates the regulation motor 30 of placing intracavity bottom surface middle part including rotating, the output shaft drive hookup of regulation motor 30 has carousel 29, carousel 29 is the cam structure, and place the intracavity wall and have baffle 28 along its radial sliding connection, baffle 28 slides and to block corresponding inlet port 26, the one end of baffle 28 and the outer anchor ring of carousel 29 are contradicted, be fixed with connecting plate 31 on the baffle 28, connecting plate 31 passes through elastic expansion mechanism 32 and places the interior wall connection in chamber, through the work of adjusting motor 30 circular telegram, drive carousel 29 is rotatory, drive baffle 28 slides, realize opening, closing of inlet port 26.
Referring to fig. 3, in order to facilitate the rotation of the rotating shaft 6, a transition mechanism is designed to ensure that the gas transmission channel normally works to transmit the mixed gas, the transition mechanism includes an annular sleeve 17 movably sleeved on the outer side of the central shaft 6 and a gas transmission pipe 16 connected to the outer ring of the annular sleeve 17, a hollow 19 is formed in the annular sleeve 17, an annular groove communicated with the hollow 19 is formed in the inner ring of the annular sleeve 17, an annular plate 20 is rotatably installed at the annular groove, and gas holes 18 communicated with the hollow 19 and the cavity 601 are fixed on the annular plate 20 and the shell of the central shaft 6.
In this embodiment, the detection module 14 includes a gas concentration sensor, a temperature sensor and a plc controller, wherein the gas concentration sensor, the temperature sensor and a signal input terminal of the plc controller are electrically connected, and a signal output terminal of the plc controller is electrically connected to an electrical component in the control assembly. Detect gas concentration and temperature value in the culture dish placed a section of thick bamboo internal environment through gas concentration sensor, temperature sensor, after gas concentration is less than the setting value, the work of plc controller control gas preparation subassembly continues to produce the mist to place the intracavity, intelligent control through gas transmission channel to each culture dish placed a section of thick bamboo.
In this embodiment, the detection module 14 further includes a humidity sensor, and the humidity sensor is electrically connected to the plc controller.
When the temperature sensor detects that the temperature in the placing cavity is lower than the set value, the liquid pump works to convey the heat preservation liquid in the placing box 5 to the interlayer 27 through the infusion channel.
The gas preparation subassembly that this device provided includes carbon dioxide preparation mechanism, and carbon dioxide preparation mechanism includes carbon dioxide generator and intensification ware, and carbon dioxide generator adopts calcium bicarbonate heating preparation, and the chemical equation of its preparation is:
and the temperature increasing device is an electric heater to provide high temperature for the gas generator and can heat the heat preservation liquid.
The embryo culture device provided by the device, when in use, the embryo culture dish is placed in the culture dish placing barrel 12, then the corresponding barrel cover 15 is closed, the gas preparation component 8 provided by the device works to release mixed gas, the mixed gas enters the hollow of each support plate 22 through the gas transmission channel, the mixed gas in the hollow enters the clamping seat and finally enters the placing cavity through the gas inlet hole, so that the concentration of the mixed gas in the placing cavity reaches a set value, the embryo is convenient to store, the mixed gas preparation component works to generate heat, the liquid in the mounting box 5 is heated, the liquid is conveyed to the inner part of the interlayer 27 of each culture dish placing barrel 12 through the liquid pump 9, the temperature in the culture dish placing barrel 12 reaches the set value, the device is also provided with the detection module 14 on the barrel cover 15 of the culture dish placing barrel 12, and the detection module 14 comprises a gas concentration sensor and a temperature sensor, the internal environment of the culture dish placing cylinder 12 can be detected conveniently in real time, real-time monitoring is facilitated, and the success rate of embryo culture is ensured.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. The utility model provides an embryo incubator which characterized in that: the cross section of an inner cavity of the box body (1) is circular, a vertical central shaft (6) is rotatably mounted at the inner center of the box body (1), a plurality of horizontally arranged support plates (22) are distributed on the outer wall of the central shaft (6) in a circumferential array manner, and a plurality of culture assemblies are mounted on the top surface of each support plate (22);
the culture assembly comprises a culture dish placing cylinder (12) and a cylinder cover (15), wherein a placing cavity and an installation cavity are respectively recessed on two sides of the culture dish placing cylinder (12), a culture dish (13) is fixed in the placing cavity, the cylinder cover (15) is used for sealing the placing cavity, a detection module (14) is installed on the cylinder cover (15), and the detection module (14) is used for detecting the internal environment of the placing cavity;
an air inlet hole (26) communicated with the placing cavity and the mounting cavity is formed in the shell of the culture dish placing cylinder (12), and an interlayer (27) is further formed in the shell of the culture dish placing cylinder (12);
a control assembly is arranged in the box body (1) and used for controlling the internal environment of the placing cavity in each culture dish placing cylinder (12).
2. An embryo incubator according to claim 1 wherein: the novel incubator is characterized in that a horizontal partition plate (11) is arranged in the incubator body (1), the control assembly is arranged below the partition plate (11), and the support plate (22) and the culture assembly are located above the partition plate (11).
3. An embryo incubator according to claim 1 wherein: control assembly is including fixing mounting box (5) in box (1) and locating gaseous preparation subassembly (8) in mounting box (5), still be equipped with locating rack (4) of fixed gaseous preparation subassembly (8) in mounting box (5), heat preservation liquid has still been stored in mounting box (5), the externally mounted of mounting box (5) has liquid pump (9), the input of liquid pump (9) communicates inside mounting box (5), the output of liquid pump (9) communicates through infusion passageway in intermediate layer (27) of each casing, discharge pipe (3) of gaseous preparation subassembly (8) stretch out box (1), the output of gaseous preparation subassembly (8) communicates through the infusion passageway in the chamber of placing of each culture dish placing section of thick bamboo (12).
4. An embryo incubator according to claim 3 wherein: the top surface distribution of extension board (22) has location cassette (25) that place a section of thick bamboo (12) with the culture dish and correspond, all is equipped with hollowly in location cassette (25) and the culture dish and places the inside intercommunication of installation cavity of a section of thick bamboo (12) bottom, all is equipped with hollow in extension board (22), and hollow inside and each location cassette (25) inside intercommunication, and the inner wall of location cassette (25) and the outer wall sealing connection that a section of thick bamboo (12) was placed to the culture dish.
5. An embryo incubator according to claim 4 wherein: infusion passageway is including connecting transfer line (10) at liquid pump (9) output, fixing branch liquid pipe (21) in the hollow and set up infusion way (24) of placing a section of thick bamboo (12) bottom surface at the culture dish, divide on liquid pipe (21) to distribute and go up infusion head (23) that cassette (25) correspond with this extension board (22), the bottom port of infusion way (24) and the terminal intercommunication that corresponds infusion head (23), wherein, the one end of dividing liquid pipe (21) is stretched out and is corresponded extension board (22) to pass through coupling mechanism with transfer line (10) and be connected.
6. An embryo incubator according to claim 1 wherein: the connecting mechanism comprises a bearing table (33) fixed on the inner wall of the box body (1), an annular temporary storage groove (36) is formed in the bearing table (33), an annular groove opening of the temporary storage groove (36) is connected with an annular sealing plate (35) in a coaxial rotating mode, one end of the liquid distribution pipe (21) extending out of the corresponding supporting plate (22) is communicated with a connector (34), the other end of the connector (34) is connected with the annular sealing plate (35) and is communicated with the temporary storage groove (36) in an internal mode, and the tail end of the infusion pipe (10) penetrates through the partition plate (11) and is communicated with the temporary storage groove (36).
7. An embryo incubator according to claim 3 or 6 wherein: the gas transmission channel comprises a cavity (601) arranged in a central shaft (6), a first communication hole arranged on a shell of the central shaft (6), one end of the first communication hole is communicated with the cavity (601), the other end of the first communication hole is communicated with the hollow part in a support plate (22), the hollow part is communicated with the inside of a clamping seat (25) on the support plate (22), the inner wall of the clamping seat is communicated with an installation cavity corresponding to a culture dish placing cylinder (12), the output end of a gas preparation assembly (8) is connected with a transition mechanism on the outer side of the central shaft (6), and the other end of the transition mechanism is communicated with the cavity (601).
8. An embryo incubator according to claim 7 wherein: the transition mechanism comprises an annular sleeve (17) movably sleeved on the outer side of the central shaft (6) and a gas conveying pipe (16) connected to the outer ring of the annular sleeve (17), wherein a hollow part (19) is formed in the annular sleeve (17), an annular groove communicated with the hollow part (19) is formed in the inner ring of the annular sleeve (17), an annular plate (20) is rotatably installed at the annular groove, and gas holes (18) communicated with the hollow part (19) and the cavity (601) are fixed on the annular plate (20) and the shell of the central shaft (6).
9. An embryo incubator according to claim 8 wherein: the detection module comprises a gas concentration sensor, a temperature sensor and a plc controller, wherein the gas concentration sensor, the temperature sensor and a signal input end of the plc controller are electrically connected, and a signal output end of the plc controller is electrically connected to an electric element in the control assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110894616.8A CN113583872B (en) | 2021-08-05 | 2021-08-05 | Embryo incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110894616.8A CN113583872B (en) | 2021-08-05 | 2021-08-05 | Embryo incubator |
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| Publication Number | Publication Date |
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| CN113583872A true CN113583872A (en) | 2021-11-02 |
| CN113583872B CN113583872B (en) | 2023-07-21 |
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| CN202110894616.8A Active CN113583872B (en) | 2021-08-05 | 2021-08-05 | Embryo incubator |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007267701A (en) * | 2006-03-31 | 2007-10-18 | Miyazaki Prefecture | Culture container, culture device and culture method |
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| CN112877214A (en) * | 2021-04-07 | 2021-06-01 | 中南大学湘雅医院 | Pressure controlled cell culture device for glaucoma research |
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| JP2007267701A (en) * | 2006-03-31 | 2007-10-18 | Miyazaki Prefecture | Culture container, culture device and culture method |
| CN205275616U (en) * | 2015-12-12 | 2016-06-01 | 内蒙古草原生态畜牧产业研究院股份有限公司 | Carbon dioxide incubator |
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| CN211999752U (en) * | 2019-12-24 | 2020-11-24 | 青岛大学附属医院 | Test tube baby embryo incubator |
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| CN113583872B (en) | 2023-07-21 |
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