CN216513911U - Low-oxygen culture device for scale production of stem cells - Google Patents
Low-oxygen culture device for scale production of stem cells Download PDFInfo
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- CN216513911U CN216513911U CN202220849417.5U CN202220849417U CN216513911U CN 216513911 U CN216513911 U CN 216513911U CN 202220849417 U CN202220849417 U CN 202220849417U CN 216513911 U CN216513911 U CN 216513911U
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Abstract
The utility model provides a hypoxia culture device for scale production of stem cells, which relates to the technical field of cell culture devices and comprises an incubator and a control unit, wherein the incubator is internally provided with a cavity and a plurality of culture chambers, each culture chamber is internally provided with a culture disc in a sliding way, one end of each culture disc extends out of the incubator through a taking and placing opening, one end of each culture disc extending out of the incubator is fixedly provided with a sealing baffle, each taking and placing opening is provided with a locking mechanism, the other end of each culture disc is provided with a popup mechanism, each culture chamber is internally provided with a guide cover, a lighting lamp and an atomizing nozzle, an air inlet main pipe and a water inlet main pipe are arranged in the cavity, the middle part of each guide cover is provided with an air inlet branch pipe communicated with the air inlet main pipe, and each atomizing nozzle is communicated with the water inlet main pipe through the water inlet branch pipe, the utility model realizes the rapid taking and placing of a culture medium, the problem of slow operation of taking and placing the culture medium is solved, and the efficiency and the quality of stem cell culture are improved.
Description
Technical Field
The utility model relates to the technical field of cell culture devices, in particular to a hypoxia culture device for large-scale production of stem cells.
Background
Hypoxic culture refers to a state of culture when the oxygen content in cells is lower than that in air, and the change of oxygen content is one of the most important regulators in the whole life cycle, and extends from embryonic development, maintenance of normal body functions, to pathological processes such as diseases and aging, wherein hypoxia is the most common basic environment.
In chinese utility model patent with patent application number 201820538680.6, a hypoxia incubator is proposed, and the main problems that exist in this patent are: in this patent, only with placing at will the culture medium in the culture chamber, operating personnel need get many times and put the operation and just can accomplish getting of whole culture medium and put, and is more troublesome, makes getting of culture medium put speed ratio slower, therefore has influenced stem cell culture's efficiency, and long-time getting of the while is put the operation and also is made the outside air enter into the culture chamber easily, also influences stem cell culture's quality easily.
Therefore, there is a need for a hypoxic culture apparatus for the large-scale production of stem cells that can solve the above problems.
SUMMERY OF THE UTILITY MODEL
The utility model provides a hypoxia culture device for large-scale production of stem cells, which realizes quick taking and placing of a culture medium, solves the problem of slow operation of taking and placing the culture medium, and improves the efficiency and quality of stem cell culture.
The technical scheme of the utility model is realized as follows:
the hypoxia culture device for the large-scale production of the stem cells comprises an incubator, wherein a cavity is vertically arranged in the middle of the incubator, and a plurality of culture chambers are respectively arranged on two sides of the cavity;
a culture disc is slidably mounted in each culture chamber along the horizontal direction, a plurality of placing grooves for placing culture media are formed in each culture disc, a taking and placing opening is formed in a side wall, far away from the cavity, of each culture chamber, one end of each culture disc extends out of the incubator through the taking and placing opening, a sealing baffle is fixed to one end, extending out of the incubator, of each culture disc, the inner wall of each sealing baffle abuts against the outer wall of the incubator, a locking mechanism for locking the sealing baffle is mounted at each taking and placing opening, a popup mechanism is mounted on a side wall, close to the cavity, of each culture chamber, and is used for pushing the culture discs to extend outwards;
each culture room is internally fixed with a horn-shaped guide cover, each guide cover is arranged right above the placement groove, an air inlet branch pipe is arranged in the middle of each guide cover, an air inlet main pipe and an air inlet main pipe are arranged in the cavity, each air inlet branch pipe is communicated with the air inlet main pipe, the air inlet main pipe and the air inlet branch pipe are used for introducing low-oxygen gas into the culture room, an illuminating lamp is arranged at the bottom of each guide cover, an atomizing nozzle is arranged on the side wall of each culture room, and each atomizing nozzle is communicated with the air inlet main pipe through the water inlet branch pipe;
also comprises a control unit.
As a preferred technical scheme, the control unit includes control panel and treater, each cultivate indoor heater, oxygen sensor, humidity transducer, light sensor and the temperature sensor of all installing, each a first solenoid valve is all installed to the inlet end of inlet branch pipe, each a second solenoid valve, each are all installed to the end of intaking of inlet branch pipe light, control panel, heater, oxygen sensor, humidity transducer, light sensor, temperature sensor, first solenoid valve and second solenoid valve all with treater electric connection.
As a preferred technical scheme, the locking mechanism includes a first mounting seat, the first mounting seat is fixed in take and put the open-ended top, be equipped with an installation cavity in the first mounting seat, install an installation pole in the installation cavity, the one end of installation pole is fixed with limit baffle, limit baffle with install a compression spring between the installation intracavity wall, a compression spring cover is located on the installation pole, the other end of installation pole stretches out first mounting seat, the one end that the installation pole stretches out first mounting seat is fixed with a locking pole, the locking pole support by in on the outer wall of seal baffle, be fixed with the supporting shoe on the first mounting seat, the supporting shoe set up in one side of locking pole.
As a preferred technical scheme, the pop-up mechanism comprises a second mounting seat fixed on the inner wall of the culture chamber, a sliding sleeve is sleeved on the second mounting seat, the sliding sleeve is slidably mounted with the second mounting seat, the end of the sliding sleeve abuts against the end of the culture disc, a second compression spring is mounted inside the sliding sleeve, one end of the second compression spring is connected with the inner wall of the sliding sleeve, and the other end of the second compression spring is connected with the second mounting seat.
As a preferred technical scheme, a sealing rubber ring is fixed on the inner wall of each sealing baffle plate, and the sealing rubber ring is tightly attached to the outer wall of the culture chamber.
As a preferred technical scheme, the water inlet main pipe is connected with a water storage tank, and a water guide pump is installed at the water inlet end of the water inlet main pipe.
In a preferred embodiment, an observation window for observing the culture condition of the stem cells in the culture medium and an exhaust pipe for exhausting the gas in the culture chamber are mounted on a side wall of each of the culture chambers.
By adopting the technical scheme, the utility model has the beneficial effects that:
because the hypoxia culture device for the large-scale production of the stem cells comprises the culture box, the cavity and the culture chamber are arranged in the culture box, and the culture disc is arranged in the culture chamber, in the hypoxia culture device, the culture medium for culturing the stem cells is placed in the placing groove on the culture disc, the culture disc is pulled out of the culture box, all the culture medium in the culture chamber can be taken and placed, the culture medium is greatly convenient for operators to take and place, the problem that the taking and placing operation is slow due to the fact that the culture medium needs to be taken and placed one by one is solved, the culture medium is taken and placed fast, and the culture efficiency of the stem cells is improved.
Because the sealing baffle is fixed at one end of each culture disc extending out of the incubator, and a locking mechanism for locking the sealing baffle is installed at each pick-and-place opening, in the utility model, the locking rod in the locking mechanism tightly presses the sealing baffle on the outer wall of the incubator, so that the culture disc is locked and the culture chamber is sealed, and the oxygen environment in the culture chamber is prevented from being influenced by the external air entering the culture chamber, thereby ensuring the culture effect of the utility model on stem cells; meanwhile, when the culture medium needs to be taken and placed, an operator can rotate the locking rod to separate the locking rod from the outer wall of the sealing baffle plate, the structure and operation of the locking mechanism are very simple, and the locking and unlocking operation of the operator is facilitated.
Because the side wall of each culture chamber close to the cavity is provided with the pop-up mechanism, when an operator rotates the locking rod to leave the sealing baffle plate, the sliding sleeve in the pop-up mechanism pushes the culture disc to move outwards under the action of the elastic force of the second compression spring, so that the operator can pull the culture disc out of the culture chamber to take and place culture medium conveniently.
Because each culture chamber is fixed with a trumpet-shaped guide cover, the guide cover is used for facilitating the hypoxic gas to be more uniformly and rapidly diffused into the whole culture chamber so as to ensure that the oxygen concentration in the culture chamber is always maintained in a proper range; the air inlet branch pipe is arranged in the middle of the guide cover, the air inlet main pipe and the water inlet main pipe are arranged in the cavity, the illuminating lamp is arranged at the bottom of the guide cover, the atomizing nozzles and the heaters are arranged on the side walls of the culture chamber, in the utility model, the air inlet main pipe and the air inlet branch pipe are used for introducing low-oxygen gas with proper oxygen concentration into the culture chamber, the atomizing nozzles are used for spraying water mist into the culture chamber to humidify and cool the culture chamber, the illuminating lamp is used for illuminating the culture chamber, and the heaters are used for heating the culture chamber to keep the culture chamber in an optimal culture temperature range, so that the culture environment in the culture chamber is ensured, and the culture quality of stem cells is further ensured.
According to the utility model, the oxygen sensor is used for monitoring the oxygen concentration in the culture chamber, and an operator can adjust the oxygen content in the hypoxic gas according to the monitored oxygen concentration data; the humidity sensor is used for monitoring the humidity in the culture chamber, when the humidity in the culture chamber is monitored to be smaller than the preset humidity, the processor can control the second electromagnetic valve and the water guide pump to be opened, and the mist-shaped spray head is used for spraying water into the culture chamber to humidify and cool the interior of the culture chamber; the illumination sensor is used for monitoring the illumination intensity in the culture chamber, and when the monitored illumination intensity is smaller than the preset illumination intensity, the processor can control the illumination lamp to be turned on to illuminate the interior of the culture chamber; temperature sensor is used for monitoring the temperature in the cultivation room, and when the temperature of monitoring was less than preset temperature, the treater can control the heater and start, heats cultivation indoor portion to make oxygen concentration, humiture and illuminance in cultivation indoor all be in the most suitable temperature range of stem cell culture, and then effectively guaranteed stem cell culture's quality.
Because the inner wall of each sealing baffle is fixed with the sealing rubber ring, the arrangement of the sealing rubber ring effectively ensures the sealing property in the culture chamber.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the inside of a culture chamber in the present invention;
fig. 3 is a schematic diagram of the connection of the control unit according to the present invention.
Wherein: 1. an incubator; 2. a cavity; 3. a culture chamber; 4. a culture tray; 5. placing a groove; 6. an access opening; 7. sealing the baffle; 8. a guide cover; 9. an intake branch pipe; 10. an intake manifold; 11. a water inlet main pipe; 12. an illuminating lamp; 13. an atomizing spray head; 14. a water inlet branch pipe; 15. a control panel; 16. a processor; 17. a heater; 18. an oxygen sensor; 19. a humidity sensor; 20. an illumination sensor; 21. a temperature sensor; 22. a first solenoid valve; 23. a second solenoid valve; 24. a first mounting seat; 25. a mounting cavity; 26. mounting a rod; 27. a limit baffle; 28. a first compression spring; 29. a locking lever; 30. a support block; 31. a second mounting seat; 32. a sliding sleeve; 33. a second compression spring; 34. sealing the rubber ring; 35. a water storage tank; 36. a priming pump; 37. an observation window; 38. and (4) exhausting the gas.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in the figures 1-3 together, the hypoxia culture device for the large-scale production of stem cells comprises an incubator 1, wherein a cavity 2 is vertically arranged in the middle of the incubator 1, and a plurality of culture chambers 3 are respectively arranged on two sides of the cavity 2.
All there is a cultivation dish 4 along horizontal direction slidable mounting in each cultivation room 3, all be equipped with the recess 5 of placing that a plurality of is used for placing the culture medium on each cultivation dish 4, it gets on and puts opening 6 all to be equipped with one on the lateral wall that cavity 2 was kept away from to each cultivation room 3, the one end of each cultivation dish 4 all stretches out incubator 1 through getting to put opening 6, the one end that each cultivation dish 4 stretches out incubator 1 all is fixed with a sealing baffle 7, the inner wall of each sealing baffle 7 all supports and leans on incubator 1's outer wall, each is got and is put opening 6 department and all installs a locking mechanical system who is used for locking sealing baffle 7, each cultivation room 3 all installs a pop-up mechanism on being close to cavity 2's the lateral wall, pop-up mechanism is used for promoting cultivation dish 4 and outwards stretches out.
In the utility model, the culture medium for culturing the stem cells is placed in the placing groove 5 on the culture disc 4, so that the whole culture medium in each culture is integrally taken and placed, great convenience is brought to the taking and placing of the culture medium by operators, the problem that the taking and placing operation is slow because the culture medium needs to be taken and placed one by one is solved, the culture medium is rapidly taken and placed, and the culture efficiency of the stem cells is improved.
All be fixed with a guide cover 8 that is the loudspeaker form in each cultivates room 3, each guide cover 8 all sets up directly over placing recess 5, an air intake branch pipe 9 is all installed at the middle part of each guide cover 8, install inlet manifold 10 and inlet manifold 11 in the cavity 2, each inlet manifold 9 all is linked together with inlet manifold 10, inlet manifold 10 and inlet branch pipe 9 are used for letting in the hypoxemia gas in 3 to cultivateing the room, light 12 is all installed to the bottom of each guide cover 8, all install atomizer 13 on the lateral wall of each cultivateing room 3, each atomizer 13 all is linked together through inlet branch pipe 14 and inlet manifold 11.
Also comprises a control unit.
Wherein, the control unit comprises a control panel 15 and a processor 16, each culture chamber 3 is internally provided with a heater 17, an oxygen sensor 18, a humidity sensor 19, a light sensor 20 and a temperature sensor 21, the air inlet end of each air inlet branch pipe 9 is provided with a first electromagnetic valve 22, the water inlet end of each water inlet branch pipe 14 is provided with a second electromagnetic valve 23, each illuminating lamp 12, the control panel 15, the heater 17, the oxygen sensor 18, the humidity sensor 19, the light sensor 20, the temperature sensor 21, the first electromagnetic valve 22 and the second electromagnetic valve 23 are electrically connected with the processor 16, in the embodiment, the processor 16 can adopt a C51 series single chip microcomputer, the control panel 15 can adopt a DL203 model control panel, the illuminating lamp 12 can adopt an LED light source, the heater 17 can adopt a commercially available electric heater, the oxygen sensor 18 can adopt an O2-A2 model oxygen sensor of English alphasense company, the temperature sensor 21 can be a DS18B20 model temperature sensor, the humidity sensor 19 can be a HOH-4602-C model humidity sensor of Honeywell, and the illumination sensor 20 can be an RS-GZ series illumination transmitter of Minandaucai.
As shown in fig. 2, the locking mechanism includes a first mounting seat 24, the first mounting seat 24 is fixed above the pick-and-place opening 6, a mounting cavity 25 is provided in the first mounting seat 24, a mounting rod 26 is installed in the mounting cavity 25, a limiting baffle 27 is fixed at one end of the mounting rod 26, a first compression spring 28 is installed between the limiting baffle 27 and an inner wall of the mounting cavity 25, the first compression spring 28 is sleeved on the mounting rod 26, the other end of the mounting rod 26 extends out of the first mounting seat 24, a locking rod 29 is fixed at one end of the mounting rod 26 extending out of the first mounting seat 24, the locking rod 29 abuts against an outer wall of the sealing baffle 7, a supporting block 30 is fixed on the first mounting seat 24, and the supporting block 30 is disposed at one side of the locking rod 29.
In addition, the ejecting mechanism includes a second mounting seat 31 fixed on the inner wall of the culture chamber 3, a sliding sleeve 32 is sleeved on the second mounting seat 31, the sliding sleeve 32 and the second mounting seat 31 are slidably mounted together, the end of the sliding sleeve 32 abuts against the end of the culture tray 4, a second compression spring 33 is mounted inside the sliding sleeve 32, one end of the second compression spring 33 is connected with the inner wall of the sliding sleeve 32, and the other end of the second compression spring 33 is connected with the second mounting seat 31, in the present invention, when an operator rotates the locking rod 29 away from the sealing baffle 7, the culture tray 4 loses the abutting force of the locking rod 29 on the sealing baffle 7, so that the sliding sleeve 32 pushes the culture tray 4 to move outwards under the elastic force of the second compression spring 33, thereby facilitating the operator to pull the culture tray 4 out of the culture chamber 3 to take and place the culture medium.
In the utility model, when stem cells are cultured, the locking rod 29 is tightly pressed on the outer wall of the sealing baffle 7 under the action of the elastic force of the first compression spring 28, so that the culture disc 4 and the sealing baffle 7 are locked; when taking and placing the culture medium, an operator firstly pulls the locking rod 29 outwards, then rotates the locking rod 29 to press the locking rod 29 on the supporting block 30, so as to unlock the culture disk 4 and the sealing baffle 7, and the sliding sleeve 32 pushes the culture disk 4 to extend outwards out of the incubator 1 under the action of the elastic force of the second compression spring 33.
In addition, a sealing rubber ring 34 is fixed on the inner wall of each sealing baffle 7, and the sealing rubber ring 34 is tightly attached to the outer wall of the culture chamber 3.
The water inlet main pipe 11 is connected with a water storage tank 35, and a water guide pump 36 is installed at the water inlet end of the water inlet main pipe 11.
An observation window 37 and an exhaust pipe 38 are mounted on the side wall of each culture chamber 3, the observation window 37 is used for observing the culture condition of stem cells in the culture medium, and the exhaust pipe 38 is used for exhausting the gas in the culture chamber 3 out of the culture chamber 3.
In conclusion, the hypoxia culture device for the large-scale production of the stem cells, provided by the utility model, realizes the quick taking and placing of the culture medium, solves the problem of slow taking and placing operation of the culture medium, and improves the efficiency and quality of stem cell culture.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. The hypoxia culture device for the large-scale production of the stem cells is characterized by comprising an incubator, wherein a cavity is vertically arranged in the middle of the incubator, and a plurality of culture chambers are respectively arranged on two sides of the cavity;
a culture disc is slidably mounted in each culture chamber along the horizontal direction, a plurality of placing grooves for placing culture media are formed in each culture disc, a taking and placing opening is formed in a side wall, far away from the cavity, of each culture chamber, one end of each culture disc extends out of the incubator through the taking and placing opening, a sealing baffle is fixed to one end, extending out of the incubator, of each culture disc, the inner wall of each sealing baffle abuts against the outer wall of the incubator, a locking mechanism for locking the sealing baffle is mounted at each taking and placing opening, a popup mechanism is mounted on a side wall, close to the cavity, of each culture chamber, and is used for pushing the culture discs to extend outwards;
each cultivation room is internally and fixedly provided with a trumpet-shaped guide cover, each guide cover is arranged right above the placement groove, the middle of each guide cover is provided with an air inlet branch pipe, an air inlet main pipe and an air inlet main pipe are arranged in the cavity, each air inlet branch pipe is communicated with the air inlet main pipe, the air inlet main pipe and the air inlet branch pipes are used for introducing hypoxic gas into the cultivation room, the bottom of each guide cover is provided with an illuminating lamp, each side wall of the cultivation room is provided with an atomizing nozzle, and each atomizing nozzle is communicated with the air inlet main pipe through the water inlet branch pipe;
also comprises a control unit.
2. The hypoxia culture device for scale production of stem cells according to claim 1, wherein the control unit comprises a control panel and a processor, each culture chamber is internally provided with a heater, an oxygen sensor, a humidity sensor, an illumination sensor and a temperature sensor, each air inlet end of each air inlet branch pipe is provided with a first electromagnetic valve, each water inlet end of each water inlet branch pipe is provided with a second electromagnetic valve, and each illuminating lamp, the control panel, the heater, the oxygen sensor, the humidity sensor, the illumination sensor, the temperature sensor, the first electromagnetic valve and the second electromagnetic valve are electrically connected with the processor.
3. The hypoxia culture device for large-scale production of stem cells according to claim 2, wherein the locking mechanism comprises a first mounting seat, the first mounting seat is fixed above the picking and placing opening, a mounting cavity is arranged in the first mounting seat, a mounting rod is arranged in the mounting cavity, a limiting baffle is fixed at one end of the mounting rod, a first compression spring is arranged between the limiting baffle and the inner wall of the mounting cavity, the first compression spring is sleeved on the mounting rod, the other end of the mounting rod extends out of the first mounting seat, a locking rod is fixed at one end of the mounting rod extending out of the first mounting seat, the locking rod abuts against the outer wall of the sealing baffle, a support block is fixed on the first mounting seat, and the support block is arranged on one side of the locking rod.
4. A hypoxia culture device for large-scale production of stem cells according to any one of claims 1 to 3, wherein the ejection mechanism comprises a second mounting seat fixed on the inner wall of the culture chamber, a sliding sleeve is sleeved on the second mounting seat, the sliding sleeve and the second mounting seat are slidably mounted together, the end of the sliding sleeve abuts against the end of the culture tray, a second compression spring is mounted inside the sliding sleeve, one end of the second compression spring is connected with the inner wall of the sliding sleeve, and the other end of the second compression spring is connected with the second mounting seat.
5. A hypoxia culture device for large-scale production of stem cells according to claim 4, wherein a sealing rubber ring is fixed on the inner wall of each sealing baffle, and the sealing rubber ring is tightly attached to the outer wall of the culture chamber.
6. A hypoxia culture device for scale production of stem cells according to claim 5, wherein the water inlet main pipe is connected with a water storage tank, and a water guide pump is installed at the water inlet end of the water inlet main pipe.
7. A hypoxic culture apparatus for large-scale production of stem cells according to claim 6, wherein an observation window and an exhaust pipe are installed on the side wall of each culture chamber, the observation window is used for observing the culture condition of stem cells in the culture medium, and the exhaust pipe is used for exhausting gas in the culture chamber out of the culture chamber.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220849417.5U CN216513911U (en) | 2022-04-14 | 2022-04-14 | Low-oxygen culture device for scale production of stem cells |
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| CN202220849417.5U CN216513911U (en) | 2022-04-14 | 2022-04-14 | Low-oxygen culture device for scale production of stem cells |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979125A (en) * | 2020-08-28 | 2020-11-24 | 安徽惠恩生物科技股份有限公司 | Serum-free cell culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979125A (en) * | 2020-08-28 | 2020-11-24 | 安徽惠恩生物科技股份有限公司 | Serum-free cell culture medium |
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