CN111979125A - Serum-free cell culture medium - Google Patents
Serum-free cell culture medium Download PDFInfo
- Publication number
- CN111979125A CN111979125A CN202010889266.1A CN202010889266A CN111979125A CN 111979125 A CN111979125 A CN 111979125A CN 202010889266 A CN202010889266 A CN 202010889266A CN 111979125 A CN111979125 A CN 111979125A
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- 239000006143 cell culture medium Substances 0.000 title claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims description 25
- 230000007246 mechanism Effects 0.000 claims description 16
- 238000007789 sealing Methods 0.000 claims description 10
- 238000009413 insulation Methods 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000002775 capsule Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Thermal Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a serum-free cell culture medium, and belongs to the field of culture media. A serum-free cell culture medium comprising: a base and a plurality of sealed cabins. The base is provided with a screw rod which is horizontally arranged, a horizontal sliding rail is arranged on the upper end face of the base and is parallel to the screw rod, one end of the screw rod is rotatably connected with a hinged support, and the hinged support is fixedly arranged on the base. A plurality of the sealed cabin is arranged along vertical direction in proper order, and two are adjacent be equipped with the chassis between the sealed cabin, the below the sealed cabin with base fixed connection, logical groove has been seted up to the lateral wall of sealed cabin, logical groove link up extremely the inner wall of sealed cabin. The sliding rail is connected with a sliding block in a sliding manner, and the screw rod penetrates through the sliding block and is in threaded connection with the sliding block; the slider is fixedly provided with a rack, and the rack is fixedly provided with a plurality of object stages.
Description
Technical Field
The invention relates to the field of culture media, in particular to a serum-free cell culture medium.
Background
In some biomedical research and treatment processes, specific cells are cultured to obtain test results or diagnostic results. When the experimental sample is large, a plurality of cell populations need to be cultured simultaneously, the test article needs to be placed at one time and sealed, and the operation efficiency is low.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a serum-free cell culture medium.
The purpose of the invention can be realized by the following technical scheme:
a serum-free cell culture medium comprising: a base and a plurality of sealed cabins.
The base is provided with a screw rod which is horizontally arranged, a horizontal sliding rail is arranged on the upper end face of the base and is parallel to the screw rod, one end of the screw rod is rotatably connected with a hinged support, and the hinged support is fixedly arranged on the base.
A plurality of the sealed cabin is arranged along vertical direction in proper order, and two are adjacent be equipped with the chassis between the sealed cabin, the below the sealed cabin with base fixed connection, logical groove has been seted up to the lateral wall of sealed cabin, logical groove link up extremely the inner wall of sealed cabin.
The sliding rail is connected with a sliding block in a sliding manner, and the screw rod penetrates through the sliding block and is in threaded connection with the sliding block; the sliding block is fixedly provided with a rack, the rack is fixedly provided with a plurality of object carrying tables, the object carrying tables are sequentially arranged along the vertical direction, and the object carrying tables are respectively in one-to-one correspondence with the sealed cabins; and a side edge is formed on the upper end surface of the object stage, corresponds to the through groove, and corresponds to the bottom surface of the sealed cabin.
The object stage can be inserted into the bottom of the sealed cabin through the through groove, and the side edge and the side wall of the sealed cabin form a closed space.
Furthermore, a driving mechanism is arranged on one side of the sealed cabin, the driving mechanism is fixedly installed on the base, the output end of the driving mechanism is fixedly connected with a rotating shaft, and a plurality of driving belt wheels are sequentially and fixedly sleeved on the rotating shaft; the driving mechanism is used for driving the rotating shaft to rotate.
The chassis is provided with a hollow inner cavity, driven belt wheels which are horizontally arranged are arranged in the inner cavity, the driven belt wheels are in one-to-one correspondence with the driving belt wheels, and a belt is sleeved between the driving belt wheels and the driven belt wheels.
A vertical driven shaft is fixedly installed on the lower end face of the driven belt wheel, and the lower end of the driven shaft extends into the sealed cabin and is rotatably connected with the sealed cabin; and a branch pipe is fixedly arranged on the side wall of the lower part of the driven shaft and is communicated with a container fixedly arranged on the chassis.
Furthermore, a sleeve is sleeved outside the driven shaft and fixedly connected with the top surface of the inside of the sealed cabin, and a plurality of mounting plates are fixedly mounted on the outer wall of the sleeve; the mounting plates are fan-shaped and are arranged in an annular array around the driven shaft; the mounting panel extends to, the top of branch pipe, the lower extreme fixed mounting of mounting panel has the heating resistor silk.
The heating device comprises a branch pipe, a plurality of baffles and a plurality of heating resistance wires, wherein the baffles are fixedly mounted on the outer wall of the branch pipe, a heat insulation plate is fixedly mounted on the upper end face of each baffle and covers the heating resistance wires, and a gap for exposing the heating resistance wires is formed between every two adjacent baffles.
Furthermore, a sealing gasket is fixedly installed on the side edge.
Further, a temperature sensor is disposed within the capsule.
Further, the outer wall of the branch pipe is attached to the heating resistance wire.
Further, the heating resistance wires are arranged in an S shape.
The invention has the beneficial effects that:
the screw rod will be rotated earlier for the objective table deviates from the sealed cabin, and the cultivation object is placed on the objective table in proper order, then rotates the screw rod once more, and the drive slider pushes the objective table into the sealed cabin, and the side aligns the block with the spout, seals the cultivation object in the sealed cabin. So far, accomplished placing and sealed of culture object, through setting up the screw rod with promoting a plurality of objective tables to objective table and logical groove cooperation realize holistic sealed, need not be equipped with external sealing mechanism and sealed action, simplified operation process, improved work efficiency.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a perspective view of the culture medium of the present application;
FIG. 2 is a perspective cross-sectional view of a culture medium of the present application;
FIG. 3 is a front cross-sectional view of the culture medium of the present application.
The parts corresponding to the reference numerals in the figures are as follows:
1. a base; 2. a baffle plate; 3. mounting a plate; 4. a driving pulley; 5. a rotating shaft; 6. a belt; 7. a chassis; 8. a driven pulley; 9. an object stage; 10. a side edge; 11. a frame; 12. a slider; 13. a hinged support; 14. sealing the cabin; 15. a screw; 16. a driven shaft.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "opening," "upper," "lower," "thickness," "top," "middle," "length," "inner," "peripheral," and the like are used in an orientation or positional relationship that is merely for convenience in describing and simplifying the description, and do not indicate or imply that the referenced component or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present invention.
As shown in fig. 1-3, a serum-free cell culture medium comprising: a base 1 and a plurality of capsules 14. The base 1 is provided with a screw rod 15 which is horizontally arranged, the upper end surface of the base 1 is provided with a horizontal sliding rail, the sliding rail is parallel to the screw rod 15, one end of the screw rod 15 is rotatably connected with a hinged support 13, and the hinged support 13 is fixedly arranged on the base 1. A plurality of the sealed cabin 14 is arranged along the vertical direction in proper order, two adjacent be equipped with the chassis 7 between the sealed cabin 14, the below the sealed cabin 14 with base 1 fixed connection, logical groove has been seted up to the lateral wall of sealed cabin 14, logical groove link up to the inner wall of sealed cabin 14.
The sliding rail is connected with a sliding block 12 in a sliding mode, and the screw rod 15 penetrates through the sliding block 12 and is in threaded connection with the sliding block 12; a rack 11 is fixedly installed on the sliding block 12, a plurality of object stages 9 are fixedly installed on the rack 11, the object stages 9 are sequentially arranged along the vertical direction, and the object stages 9 are respectively in one-to-one correspondence with the sealed cabins 14; a side edge 10 is formed on the upper end surface of the object stage 9, the side edge 10 corresponds to the through groove, and the object stage 9 corresponds to the bottom surface of the sealed cabin 14. The object stage 9 can penetrate into the bottom of the sealed cabin 14 through the through groove, and the side edge 10 and the side wall of the sealed cabin 14 form a closed space.
In practical use, the screw 15 is rotated to make the object stage 9 come out of the sealed chamber 14, the objects to be cultured are placed on the object stage 9 in sequence, then the screw 15 is rotated again to drive the slide block 12 to push the object stage 9 into the sealed chamber 14, and the side 10 is aligned with and engaged with the sliding groove to seal the objects to be cultured in the sealed chamber 14. By this, the culture object is placed and sealed, the screw 15 is arranged to push the object stages 9, the object stages 9 are matched with the through grooves, the whole sealing is realized, external sealing mechanisms and sealing actions are not needed, the operation process is simplified, and the working efficiency is improved.
Furthermore, a driving mechanism is arranged on one side of the sealed cabin 14, the driving mechanism is fixedly installed on the base 1, an output end of the driving mechanism is fixedly connected with a rotating shaft 5, and a plurality of driving belt wheels 4 are sequentially and fixedly sleeved on the rotating shaft 5; the driving mechanism is used for driving the rotating shaft 5 to rotate. The chassis 7 is provided with a hollow inner cavity, driven belt wheels 8 which are horizontally arranged are arranged in the inner cavity, the driven belt wheels 8 correspond to the driving belt wheels 4 one by one, and a belt 6 is sleeved between the driving belt wheels 4 and the driven belt wheels 8. A vertical driven shaft 16 is fixedly mounted on the lower end face of the driven pulley 8, and the lower end of the driven shaft 16 extends into the sealed cabin 14 and is rotatably connected with the sealed cabin 14; and a branch pipe is fixedly arranged on the side wall of the lower part of the driven shaft 16 and is communicated with a container fixedly arranged on the chassis 7.
That is, when extra reagent is needed to be added to multiple cultures on the object stage 9 during the culture process, the reagent stored in the container on the bottom plate 7 can be directly discharged to the culture object on the object stage 9 through the branch pipes, the number and arrangement of the branch pipes correspond to those of the cultures on the object stage 9, and therefore the rotating shaft 5 is also rotated by starting the driving mechanism, and the driven shaft 16 is driven to rotate, so that the outlets of the branch pipes are aligned with the culture object.
Further, a sleeve is sleeved outside the driven shaft 16 and fixedly connected with the inner top surface of the sealed cabin 14, and a plurality of mounting plates 3 are fixedly mounted on the outer wall of the sleeve; the mounting plate 3 is fan-shaped and arranged in an annular array around the driven shaft 16; the mounting plate 3 extends to the upper part of the branch pipe, and the lower end of the mounting plate 3 is fixedly provided with a heating resistance wire. The heating device is characterized in that a plurality of baffles 2 are fixedly mounted on the outer wall of the branch pipe, a heat insulation plate is fixedly mounted on the upper end face of each baffle 2 and covers the heating resistance wires, and a gap for exposing the heating resistance wires is formed between every two adjacent baffles 2.
That is, the temperature of the capsule 14 can be raised by energizing the resistance heater. When the heat insulation plate partially covers the heating resistance wire, the heat energy of the heating resistance wire cannot be directly radiated into the sealed cabin 14, and the sealed cabin 14 is heated. The heat-conducting wires can be additionally arranged on the heat-insulating plate to conduct heat to the outside of the sealing plate. When the driving mechanism rotates the rotating shaft 5, the driven shaft 16 rotates, and when the baffle 2 driving the branch pipes rotates, the overlapping part of the mounting plate 3 and the baffle 2 can be adjusted, so that the exposed length of the heating resistance wire can be adjusted, and the heating rate of the sealed cabin 14 can be adjusted.
Furthermore, a sealing gasket is fixedly mounted on the side edge 10, so that the sealing performance is improved.
Further, a temperature sensor is arranged in the sealed cabin 14, and a temperature value in the sealed cabin 14 is obtained.
Furthermore, the outer wall of the branch pipe is attached to the heating resistance wire, so that the branch pipe can be heated through the heating resistance wire, the added temperature actually reaches a proper temperature to adapt to the temperature of the culture object, and the temperature environment of the culture object is prevented from being interfered after the reagent is added.
Furthermore, the heating resistance wires are arranged in an S shape, so that the contact area is increased.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.
Claims (7)
1. A serum-free cell culture medium comprising: a base and a plurality of sealed cabins;
the base is provided with a screw rod which is horizontally arranged, the upper end surface of the base is provided with a horizontal sliding rail, the sliding rail is parallel to the screw rod, one end of the screw rod is rotatably connected with a hinged support, and the hinged support is fixedly arranged on the base;
the plurality of sealed cabins are sequentially arranged along the vertical direction, a chassis is arranged between two adjacent sealed cabins, the sealed cabin at the lowest part is fixedly connected with the base, a through groove is formed in the side wall of the sealed cabin, and the through groove penetrates through the inner wall of the sealed cabin;
the sliding rail is connected with a sliding block in a sliding manner, and the screw rod penetrates through the sliding block and is in threaded connection with the sliding block; the sliding block is fixedly provided with a rack, the rack is fixedly provided with a plurality of object carrying tables, the object carrying tables are sequentially arranged along the vertical direction, and the object carrying tables are respectively in one-to-one correspondence with the sealed cabins; a side edge is formed on the upper end surface of the object stage, the side edge corresponds to the through groove, and the object stage corresponds to the bottom surface of the sealed cabin;
the object stage can be inserted into the bottom of the sealed cabin through the through groove, and the side edge and the side wall of the sealed cabin form a closed space.
2. The serum-free cell culture medium according to claim 1, wherein a driving mechanism is arranged on one side of the sealed cabin, the driving mechanism is fixedly installed on the base, an output end of the driving mechanism is fixedly connected with a rotating shaft, and a plurality of driving pulleys are sequentially and fixedly sleeved on the rotating shaft; the driving mechanism is used for driving the rotating shaft to rotate;
the chassis is provided with a hollow inner cavity, driven belt wheels which are horizontally arranged are arranged in the inner cavity, the driven belt wheels correspond to the driving belt wheels one by one, and a belt is sleeved between the driving belt wheels and the driven belt wheels;
a vertical driven shaft is fixedly installed on the lower end face of the driven belt wheel, and the lower end of the driven shaft extends into the sealed cabin and is rotatably connected with the sealed cabin; and a branch pipe is fixedly arranged on the side wall of the lower part of the driven shaft and is communicated with a container fixedly arranged on the chassis.
3. The serum-free cell culture medium according to claim 2, wherein a sleeve is sleeved outside the driven shaft and fixedly connected with the inner top surface of the sealed cabin, and a plurality of mounting plates are fixedly mounted on the outer wall of the sleeve; the mounting plates are fan-shaped and are arranged in an annular array around the driven shaft; the mounting plate extends to a position above the branch pipe, and the lower end of the mounting plate is fixedly provided with a heating resistance wire;
the heating device comprises a branch pipe, a plurality of baffles and a plurality of heating resistance wires, wherein the baffles are fixedly mounted on the outer wall of the branch pipe, a heat insulation plate is fixedly mounted on the upper end face of each baffle and covers the heating resistance wires, and a gap for exposing the heating resistance wires is formed between every two adjacent baffles.
4. The serum-free cell culture medium according to claim 1, wherein the side edge is fixedly provided with a sealing gasket.
5. The serum-free cell culture medium according to claim 3, wherein a temperature sensor is arranged within the sealed chamber.
6. The serum-free cell culture medium according to claim 3, wherein the outer wall of the branch pipe is attached to the heating resistance wire.
7. The serum-free cell culture medium according to claim 3, wherein the heating resistance wire is arranged in an S shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010889266.1A CN111979125A (en) | 2020-08-28 | 2020-08-28 | Serum-free cell culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010889266.1A CN111979125A (en) | 2020-08-28 | 2020-08-28 | Serum-free cell culture medium |
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| Publication Number | Publication Date |
|---|---|
| CN111979125A true CN111979125A (en) | 2020-11-24 |
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| CN202010889266.1A Pending CN111979125A (en) | 2020-08-28 | 2020-08-28 | Serum-free cell culture medium |
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Application publication date: 20201124 |