CN111454836B

CN111454836B - Marine bacteria culture device

Info

Publication number: CN111454836B
Application number: CN202010290244.3A
Authority: CN
Inventors: 张莉; 王殿坤
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2020-04-14
Filing date: 2020-04-14
Publication date: 2023-01-20
Anticipated expiration: 2040-04-14
Also published as: CN111454836A

Abstract

The invention relates to the technical field of marine bacteria culture, in particular to a marine bacteria culture device, which comprises a shell, an incubator, a culture chamber for bacterial culture, a gas treatment device for controlling air composition in the culture chamber, and an observation device for primary observation of bacteria; a plurality of not intercommunicated culture chambers are horizontally arranged in the incubator, the gas treatment device is respectively communicated with each culture chamber through a first cavity and a second cavity in the center of the incubator, and the observation device comprises an observation mirror arranged at the top of the shell. According to the invention, the culture chambers are respectively arranged in an isolated manner, so that different culture environments can be conveniently arranged; the culture chamber can realize rotation and vibration while meeting the requirement of bacterial culture, meets various culture requirements, reduces the number of devices used in the process and is convenient to operate; through set up viewing device in culture apparatus, can accomplish the initial observation of result in the incubator, reduce culture apparatus's the number of times of opening, avoid destroying the indoor cultivation condition of cultivateing.

Description

Marine bacteria culture device

Technical Field

The invention relates to the technical field of marine bacteria culture, in particular to a marine bacteria culture device.

Background

When marine bacteria are sampled and cultured, various instruments and equipment are needed due to complicated operation steps, so that the operation is complex, time-consuming and high in cost. Different culture conditions are usually needed to be adopted to compare culture results during bacterial culture, multiple observations need to be carried out during the culture process, but the culture conditions of the existing common equipment are single, and the culture dish needs to be taken out during the observation, so that the culture conditions in the culture device can be damaged.

Disclosure of Invention

The invention aims to solve the problems and provides a marine bacteria culture device, which can realize rotation and vibration of a culture chamber while culturing bacteria and has the functions of a bacteria culture box and a shaking table; the culture dish is arranged in a partition mode, so that a contrast experiment is convenient to arrange; set up viewing device in culture apparatus simultaneously, can directly carry out preliminary result and judge, effectively avoid destroying the problem of cultivateing the environment because of the inspection. The technical scheme is as follows:

a marine bacteria culture device comprises a shell, an incubator, a culture chamber for bacteria culture, a gas treatment device for controlling the air composition in the culture chamber, and an observation device for primary observation of bacteria; the incubator is arranged in the shell, a plurality of culture chambers which are not communicated with each other are horizontally arranged in the incubator, and the gas treatment device is respectively communicated with the culture chambers through a first cavity and a second cavity in the center of the incubator; the observation device comprises an observation mirror arranged at the top of the shell.

Preferably, a circular chassis is arranged below the incubator, a plurality of rollers are uniformly distributed below the chassis along the circumferential direction, the rollers are placed in corresponding slide ways of a planker in the shell, a stepping motor is vertically arranged on the planker, and an output shaft of the stepping motor is fixedly connected with the chassis; the chassis top sets up a plurality of vertical baffles along circumference, the baffle radially sets up along the chassis, places fan-shaped cultivation room between adjacent baffle, cultivates the room top and sets up sealed lid, and the incubator top sets up the lid.

On the basis of the scheme, the bottom plate is arranged at the bottom of the shell, the bottom plate motor is arranged on the bottom plate, an output shaft of the bottom plate motor is connected with a bottom plate screw rod, a bottom plate sliding block is arranged between the bottom plate screw rod and a bottom plate sliding rod parallel to the bottom plate screw rod, and the bottom plate sliding block is fixedly connected with the dragging plate.

Preferably, the first cavity is connected with a first air pump through a first through hole and a first air pipe on the cover body, the second cavity is connected with a second air pump through a second through hole and a second air pipe on the cover body, and the first air pipe and the second air pipe are respectively provided with a control valve; the first through hole and the second through hole are formed in a top cover in the center of the cover body, and the top cover is connected with the cover body through a bearing.

Preferably, the observation device comprises a first motor arranged at the top of the shell, an output shaft of the first motor is fixedly connected with a first screw rod, the first screw rod is arranged in parallel with the first slide bar, and a second screw rod is arranged between the first screw rod and the first slide bar and is connected with an output shaft of the second motor; the observation mirror is fixedly connected with a second sliding block on the second screw rod; transparent lid and sealed lid are set up respectively to incubator and culture room top, the shell top is provided with transparent observation window.

Preferably, be provided with the tray in the incubator, set up a plurality of constant head tanks that are used for placing the culture dish and the stopper of fastening culture dish on the tray.

On the basis of the scheme, the limiting block comprises a pressing plate, and a connecting column, a spring and a limiting sheet which are sequentially connected with one end of the pressing plate.

Preferably, a pulse electromagnet is arranged inside the tray.

Preferably, a lifting motor is arranged at the bottom of the culture chamber, an output shaft of the lifting motor is connected with a lifting screw rod, and a lifting slide block integrally formed with the tray is connected onto the lifting screw rod.

Preferably, a temperature sensor is arranged in the culture chamber, a temperature controller and a heating wire are arranged at the bottom of the tray, and the temperature controller is respectively connected with the temperature sensor and the heating wire; and an electronic display screen is arranged at the top of the shell.

The invention has the beneficial effects that:

1. the culture chambers are respectively arranged in an isolated manner, so that different culture environments including different temperatures and different air oxygen contents can be conveniently arranged, and the experimental efficiency is improved;

2. the culture room can realize rotation and vibration while meeting the requirement of bacterial culture, so that the culture device has the functions of a shaking table and a vibration incubator, meets various culture requirements, reduces the number of devices used in the process and is convenient to operate;

3. set up viewing device in culture apparatus to realize can accomplishing the preliminary observation and the judgement of result in the incubator, reduce culture apparatus's the number of times of opening, avoid destroying the indoor cultivation condition of cultivateing.

Drawings

FIG. 1: the invention has a schematic structure;

FIG. 2: the incubator of the invention is in a state of being moved out of the shell;

FIG. 3: the invention discloses a structural schematic diagram of an observation device;

FIG. 4: the structure of the culture chamber is shown schematically;

FIG. 5: the structure schematic diagram of the limiting block is provided;

FIG. 6: the installation position of the pulse electromagnet is schematic;

FIG. 7: the structure of the gas processing device is shown schematically;

FIG. 8: the cover body structure of the invention is shown schematically;

FIG. 9: the invention relates to a first cavity and a second cavity structure plan view;

FIG. 10: the invention is a structural schematic diagram of a roller and a slideway;

FIG. 11: the invention is a structural schematic diagram of a bottom plate.

Detailed Description

The invention is further illustrated by the following examples in conjunction with the accompanying drawings:

in the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the description of the present invention, it is to be understood that the terms "center", "length", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

As shown in fig. 1 to 11, a marine bacteria culture apparatus comprises a housing 1, an incubator 2, a culture chamber 3 for bacteria culture, a gas treatment device for controlling the air composition in the culture chamber 3, and an observation device for preliminary observation of bacteria; the incubator 2 is arranged in the shell 1, a plurality of culture chambers 3 which are not communicated with each other are horizontally arranged in the incubator 2, and the gas treatment device is respectively communicated with each culture chamber 3 through a first cavity 24 and a second cavity 25 in the center of the incubator 2; the viewing means comprises a viewing mirror 57 arranged at the top of the housing 1.

Preferably, as shown in fig. 4 and 10, a circular chassis 21 is arranged below the incubator 2, a plurality of rollers 22 are uniformly distributed below the chassis 21 along the circumferential direction, the rollers 22 are placed in corresponding slide ways 17 of a carriage 16 in the housing 1, a stepping motor is vertically arranged on the carriage 16, and an output shaft of the stepping motor is fixedly connected with the chassis 21, so that the incubator 2 can horizontally rotate under the driving of the stepping motor, and is supported by the rollers 22 and the rotation resistance is reduced; a plurality of vertical partition plates 23 are arranged above the chassis 21 along the circumferential direction, the partition plates 23 are arranged along the radial direction of the chassis 21, the sector culture chambers 3 are arranged between the adjacent partition plates 23, and the culture chambers 3 are not communicated with each other, so that different condition settings are realized; the top of the culture chamber 3 is provided with a sealing cover 37, and the top of the incubator 2 is provided with a cover body 26, so that when the tightness is ensured, unnecessary culture chambers are prevented from being opened when the culture dishes are taken and placed, and the culture environment in the culture chambers is influenced.

On the basis of the above scheme, as shown in fig. 2 and fig. 11, a bottom plate 11 is arranged at the bottom of the housing 1, a bottom plate motor 12 is arranged on the bottom plate 11, an output shaft of the bottom plate motor 12 is connected with a bottom plate screw 13, a bottom plate slider 15 is arranged between the bottom plate screw 13 and a bottom plate slide rod 14 parallel to the bottom plate screw 13, and the bottom plate slider 15 is fixedly connected with the planker 16.

Preferably, as shown in fig. 7 to 9, the first cavity 24 is connected to the first air pump 42 through a first through hole 263 and a first air pipe 41 on the cover 26, the second cavity 25 is connected to the second air pump 44 through a second through hole 264 and a second air pipe 43 on the cover 26, the first air pipe 41 and the second air pipe 43 are respectively provided with a control valve 45, and the air in the culture chamber 3 is pumped and discharged through the first air pump 42 and the second air pump 44 according to experimental requirements, so as to adjust the conditions of the oxygen content of the air in the culture environment. In order to facilitate opening and closing of the cover 26, a section of corrugated pipe is disposed in each of the first air pipe 41 and the second air pipe 43. The first through hole 263 and the second through hole 264 are arranged on a top cover 262 at the center of the cover 26, and the top cover 262 is connected with the cover 26 through a bearing 261, so that the air pipes are prevented from rotating together when the incubator 2 rotates. To increase the sealing of the incubator 2, the lid 26 is provided with an annular flange at its edge, the lower edge of which is lower than the upper edge of the side wall of the incubator 2.

In order to enlarge the observation range of the observation mirror 57, as shown in fig. 1 and 3, the observation device further includes a first motor 51 disposed at the top of the housing 1, an output shaft of the first motor 51 is fixedly connected to a first screw 52, the first screw 52 is disposed in parallel with the first slide bar 53, and a second screw 55 is disposed between the first screw 52 and the first slide bar 53 and is connected to an output shaft of a second motor 54; the observation mirror 57 is fixedly connected with the second slide block 56 on the second screw 55. With the above configuration, the observation mirror 57 can be moved in the horizontal plane. During observation, the chassis 21 of the incubator 2 is rotated by driving the stepping motor on the bottom plate 11, so that the culture dishes in different culture chambers 3 are moved to the observation range of the observation mirror 57. For the convenience of observation, a transparent cover 26 and a sealing cover 37 are respectively arranged above the incubator 2 and the culture chamber 3, and a transparent observation window 19 is arranged on the top of the shell 1.

As shown in fig. 4, a tray 31 is arranged in the culture chamber 3, a plurality of positioning slots 35 for placing the culture dish and a plurality of limiting blocks 36 for fastening the culture dish are arranged on the tray 31, and the structure of the limiting blocks 36 is as shown in fig. 5, and the limiting blocks 36 include a pressing plate 361, and a connecting column 362, a spring 363 and a limiting sheet 364 which are connected with one end of the pressing plate 361 in sequence, and the spring 363 and the limiting sheet 364 are rotatably arranged in the tray 31. After the petri dish is placed in the positioning slot 35, the pressing plate 361 is lifted and rotated to the petri dish, and the pressing plate 361 is pressed above the petri dish by the stretching action of the spring 363.

In addition to the above-mentioned solution, as shown in fig. 6, the tray 31 has an internal hollow structure, a pulse electromagnet 32 is provided in the tray 31, and the tray 31 is vibrated by the action of the pulse electromagnet 32. A temperature sensor is arranged in the culture chamber 3, a temperature controller and an electric heating wire are arranged at the bottom of the tray 31, and the temperature controller is respectively connected with the temperature sensor and the electric heating wire; an electronic display screen 18 is arranged on the top of the shell 1 and used for displaying the condition data of the temperature, the air pressure, the oxygen content and the like of each culture chamber 3 in real time.

In order to improve the observation effect of the observation mirror 57, the bottom of the culture chamber 3 is provided with a lifting motor, an output shaft of the lifting motor is connected with a lifting screw 33, the lifting screw 33 is connected with a lifting slide block 34 which is integrally formed with the tray 31, and the height position of the tray 31 is adjusted by driving the lifting motor, so that the distance between the tray and the observation mirror 57 is adjusted according to the height of a culture dish or other culture container.

The present invention has been described above by way of example, but the present invention is not limited to the above-described specific embodiments, and any modification or variation made based on the present invention is within the scope of the present invention as claimed.

Claims

1. A marine bacteria culture device is characterized by comprising a shell (1), an incubator (2), a culture chamber (3) for bacteria culture, a gas treatment device for controlling air composition in the culture chamber (3), and an observation device for primary observation of bacteria; the incubator (2) is arranged in the shell (1), a plurality of culture chambers (3) which are not communicated with each other are horizontally arranged in the incubator (2), and the gas treatment device is respectively communicated with the culture chambers (3) through a first cavity (24) and a second cavity (25) in the center of the incubator (2); the observation device comprises an observation mirror (57) arranged at the top of the shell (1);

the observation device comprises a first motor (51) arranged at the top of the shell (1), an output shaft of the first motor (51) is fixedly connected with a first screw rod (52), the first screw rod (52) is arranged in parallel with a first sliding rod (53), and a second screw rod (55) is arranged between the first screw rod (52) and the first sliding rod (53) and is connected with an output shaft of a second motor (54); the observation mirror (57) is fixedly connected with a second sliding block (56) on the second screw rod (55); a transparent cover body (26) and a sealing cover (37) are respectively arranged above the incubator (2) and the culture chamber (3), and a transparent observation window (19) is arranged at the top of the shell (1);

a tray (31) is arranged in the culture chamber (3), and a plurality of positioning grooves (35) for placing culture dishes and limiting blocks (36) for fastening the culture dishes are arranged on the tray (31).

2. The marine bacteria culture device according to claim 1, characterized in that a circular chassis (21) is arranged below the incubator (2), a plurality of rollers (22) are uniformly distributed below the chassis (21) along the circumferential direction, the rollers (22) are placed in corresponding slide ways (17) of a carriage (16) in the housing (1), a stepping motor is vertically arranged on the carriage (16), and an output shaft of the stepping motor is fixedly connected with the chassis (21); a plurality of vertical partition plates (23) are arranged above the base plate (21) along the circumferential direction, the partition plates (23) are radially arranged along the base plate (21), a fan-shaped culture chamber (3) is arranged between the adjacent partition plates (23), a sealing cover (37) is arranged at the top of the culture chamber (3), and a cover body (26) is arranged at the top of the culture box (2).

3. The marine bacteria culture device according to claim 2, wherein a bottom plate (11) is arranged at the bottom of the housing (1), a bottom plate motor (12) is arranged on the bottom plate (11), an output shaft of the bottom plate motor (12) is connected with a bottom plate screw rod (13), a bottom plate sliding block (15) is arranged between the bottom plate screw rod (13) and a bottom plate sliding rod (14) parallel to the bottom plate screw rod, and the bottom plate sliding block (15) is fixedly connected with the carriage (16).

4. The marine bacteria culture device of claim 2, wherein the first cavity (24) is connected with a first air pump (42) through a first through hole (263) and a first air pipe (41) on the cover body (26), the second cavity (25) is connected with a second air pump (44) through a second through hole (264) and a second air pipe (43) on the cover body (26), and the first air pipe (41) and the second air pipe (43) are respectively provided with a control valve (45); the first through hole (263) and the second through hole (264) are arranged on a top cover (262) in the center of the cover body (26), and the top cover (262) is connected with the cover body (26) through a bearing (261).

5. The marine bacteria culture device of claim 1, wherein the limiting block (36) comprises a pressing plate (361), and a connecting column (362), a spring (363) and a limiting sheet (364) which are sequentially connected with one end of the pressing plate (361).

6. A marine bacteria culture device according to claim 1, wherein the tray (31) is internally provided with a pulsed electromagnet (32).

7. The marine bacteria culture device according to claim 1, wherein a lifting motor is arranged at the bottom of the culture chamber (3), an output shaft of the lifting motor is connected with a lifting screw (33), and a lifting slide block (34) which is integrally formed with the tray (31) is connected onto the lifting screw (33).

8. A marine bacteria culture device according to claim 1, wherein a temperature sensor is arranged in the culture chamber (3), a temperature controller and a heating wire are arranged at the bottom of the tray (31), and the temperature controller is respectively connected with the temperature sensor and the heating wire; an electronic display screen (18) is arranged at the top of the shell (1).