CN110713932A

CN110713932A - Equipment is cultivateed to cell temporarily

Info

Publication number: CN110713932A
Application number: CN201911246036.7A
Authority: CN
Inventors: 陈龙刚
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-12-07
Filing date: 2019-12-07
Publication date: 2020-01-21

Abstract

The invention discloses temporary cell culture equipment, which belongs to the technical field of cell culture and comprises a base, an incubator, a cell condition observation mechanism, a culture dish storage mechanism, a cell dish cleaning mechanism and a damping mechanism, wherein the top of the incubator is provided with a sealing cover in clamping fit with the incubator, one side of the incubator is provided with a hot water temperature control assembly, two ultraviolet sterilizing lamps are arranged inside the incubator, and the damping mechanism comprises a damping buffer assembly, an installation bottom plate and two anti-shaking assemblies respectively arranged on two sides of the installation bottom plate. The cell condition observation mechanism has the advantages that the cell condition observation mechanism can observe cells in each culture dish, the culture dishes can be observed in the operation groove through the microscope without opening the sealing cover, the damping and buffering operation on the heat insulation box can be realized under the action of the damping mechanism, and the condition that the culture box is easy to damage the stored cells in the moving process is avoided.

Description

Equipment is cultivateed to cell temporarily

Technical Field

The invention relates to the technical field of cell culture, in particular to a temporary cell culture device.

Background

The optimal growth condition of most cells in vitro is 37 ℃, the carbon dioxide concentration is 5%, a carbon dioxide incubator is needed for culture, once power failure or equipment failure occurs for a long time, certain damage can be caused to the cells, and the cells can be directly killed seriously. The transportation of the cells is generally adopted when the outdoor temperature exceeds 20 ℃, after the cells are subcultured and attached to the wall, the cells are transported by filling the cell culture solution into a cell transport bottle, and the cells are generally frozen and stored at the temperature below 20 ℃ and transported in a liquid nitrogen tank.

The invention patent with publication number CN109022280A discloses a cell temporary culture device, which relates to the field of cell culture devices and comprises a culture box, wherein a box cover is hinged on the culture box, a boss is fixed at the bottom of an inner cavity, the boss is in threaded connection with a protective cover, a needle body is fixed at the center position of the boss, a vent hole is arranged on the protective cover, a through hole is arranged on the outer wall of the culture box, a bin body is hinged at the through hole and rotates at the through hole, an outer plate is arranged on the outer wall of the bin body, a plurality of heat dissipation holes are arranged on one side of the bin body opposite to the outer plate, the area of the outer plate is larger than that of the through hole, and is provided with an extension part outside the through hole, an inverted U-shaped inner sealing strip is fixed on the inner side of the extension part, an inverted U-shaped outer sealing strip is fixed on, the complete culture bottle does not need to be additionally added, the cells can be directly cultured after being taken, or the subsequent experiment is carried out, so that the experiment efficiency is improved.

However, the above apparatus still has the following problems: firstly, cells need to be detected at regular time in the conveying process to detect the survival condition of the cells, and the accidental condition of the cells in the culture process is avoided, but the device can not directly detect the cells in the incubator, the cells need to be taken out and observed by using a microscope, and the cells are easy to contact with the outside in the cell taking-out process and need to be improved; secondly, the above-mentioned device can not play the effect of shock attenuation buffering to the incubator, makes the incubator cause the damage to the cell of depositing easily in the in-process that removes, and influence the experimental result of cell.

Disclosure of Invention

The invention aims to provide a cell temporary culture device to solve the technical problems that cells in an incubator cannot be directly detected and the incubator cannot be damped and buffered in the prior art.

In order to solve the technical problems, the invention provides the following technical scheme: the cell condition monitoring device comprises a base, an incubator, a cell condition observation mechanism, a culture dish storage mechanism, a cell dish cleaning mechanism and a damping mechanism arranged at the bottom end in the incubator, wherein the incubator is arranged at the top of the base, a sealing cover matched with the incubator in a clamping manner is arranged at the top of the incubator, a handle is arranged at the top of the sealing cover, the cell condition observation mechanism is arranged at the front part of the incubator, a hot water temperature control component is arranged at one side of the incubator, the culture dish storage mechanism is arranged at the top of the damping mechanism, the cell dish cleaning mechanism is arranged on the inner wall of the incubator, two ultraviolet sterilizing lamps are arranged in the incubator, the two ultraviolet sterilizing lamps are respectively arranged at two sides in the incubator, the damping mechanism comprises a damping buffer component, a horizontally arranged mounting base plate and two anti-shaking components respectively arranged at two sides of the mounting base plate, the shock absorption and buffer assembly is arranged at the bottom of the mounting bottom plate.

Further, the shock attenuation buffering subassembly sets up in mounting panel bottom and the shock attenuation part that the structure is the same including two intervals, every the shock attenuation part all including the erection column of two vertical settings and two respectively with two erection column sliding fit's slip post, the mounting panel sets up the top at two slip posts, the inside of erection column is equipped with damping spring, damping spring's both ends are connected respectively in the inside bottom of erection column and the bottom of slip post.

Further, every shock attenuation part still all includes horizontal pole and two intervals and sets up the extension board in the mounting plate bottom, the horizontal pole level sets up between two extension boards, the middle part of horizontal pole is equipped with the partition riser, the cover is equipped with two buffer blocks rather than sliding fit on the horizontal pole, every all be equipped with rather than articulated bumper bar on the buffer block, two all be equipped with the mount pad on the lateral wall of erection column, two the bumper bar is articulated with two mount pads respectively, it all is equipped with two first buffer spring to correspond every buffer block on the horizontal pole, the buffer block sets up between two first buffer spring.

Furthermore, each anti-shaking assembly comprises a side plate arranged on one side of the mounting base plate and two second buffer springs arranged at intervals, and two ends of each second buffer spring are connected to the side wall of the incubator and the side wall of the side plate respectively.

Further, the mechanism is deposited to culture dish is the spacing ring that the circumference distributes and is bearing the dish top including the bearing dish and a plurality of that rotating electrical machines, level set up, bear the dish and make for heat conducting material, the bottom of bearing the dish is equipped with the rotation post of vertical setting, the bottom of rotating the post is passed through the bearing with mounting plate's top and is connected, mounting plate's top is equipped with the connecting seat that two intervals set up, two be equipped with between the connecting seat and rotate the worm of being connected with both, the cover is equipped with the turbine with worm meshing on rotating the post, the output of rotating electrical machines and the one end fixed connection of worm.

Further, cell dish wiper mechanism including rotate motor, lifting unit, support frame, axis of rotation, vertical setting the slide rail and with slide rail sliding fit's sliding block, the support frame sets up on the lateral wall of sliding block, the top of axis of rotation can the vertical setting of pivoted on the support frame, rotate the motor setting at the top of support frame, and the output of rotating the motor and the top fixed connection of axis of rotation, be equipped with the washing pipeline on the incubator, be equipped with the washing nozzle on the play liquid end of washing pipeline, be equipped with first solenoid valve on the washing pipeline, the bottom of axis of rotation is equipped with the cleaning brush.

Further, the lifting component comprises a lifting motor and a rotating shaft which is arranged on the incubator and is horizontally arranged, a driving wheel is sleeved on the rotating shaft, a driving rod which is hinged with the driving wheel is arranged on the driving wheel, the end part of the driving rod is hinged with the side wall of the sliding block, the lifting motor is arranged on the side wall of the incubator, and the output end of the lifting motor is fixedly connected with the end part of the rotating shaft.

Further, cell situation observation mechanism is including setting up the observation cover in incubator inside, observes to cover and is equipped with the operation groove, it makes for transparent material to observe the cover, the bottom of observing the cover is equipped with the operation gloves that two symmetries set up, the below of observing the cover is equipped with the support of setting on the incubator inner wall, be equipped with the microscope on the support, one side of incubator is equipped with the feed liquor pipeline that extends to incubator inside, be equipped with the second solenoid valve on the feed liquor pipeline.

Furthermore, the hot water temperature control assembly comprises a heat preservation bag arranged at the bottom of the bearing plate and a heat preservation box arranged on the side wall of the incubator, a heater is arranged in the heat preservation box, a water inlet pipeline and a backflow pipeline are arranged on the heat preservation bag, a liquid conveying pipeline communicated with the interior of the heat preservation box is arranged at the top of the heat preservation box, a backflow pump and a liquid inlet pump are arranged below the heat preservation box, a water inlet corrugated hose is arranged between the output end of the liquid inlet pump and the water inlet pipeline, the input end of the liquid inlet pump is communicated with the bottom of the heat preservation box, the output end of the backflow pump is communicated with the upper section of the interior of the heat preservation box through a hose, and a backflow corrugated hose is.

Compared with the prior art, the invention has the beneficial effects that:

the incubator comprises an incubator body, a mounting base plate and two anti-shaking assemblies, wherein the incubator body is provided with a plurality of insulation boxes, the incubator body is provided with a plurality of sliding columns, the incubator body is provided with a plurality of anti-shaking assemblies, the first damping spring is arranged on the mounting base plate, the second damping spring is arranged on the mounting base plate, the first damping spring is connected with the second damping spring, the second damping spring is connected with the first damping spring, the second damping spring is connected with the second damping spring, and the first damping spring is connected with the second damping spring; in order to solve the technical problem that the effect of shock absorption and buffering can not be played to the incubator in the prior art.

The cell condition observation mechanism comprises an observation cover arranged in the incubator, the observation cover is made of transparent materials, two operating gloves which are symmetrically arranged are arranged at the bottom of the observation cover, cells in each culture dish can be observed through the cell condition observation mechanism, the culture dishes can be observed through a microscope in an operating groove without opening a sealing cover, an operator can adjust the microscope through the operating gloves, a plurality of cell dishes on the top of a bearing plate can rotate through the culture dish storage mechanism, each cell dish can be sequentially positioned below a liquid inlet pipeline, each cell dish can be sequentially moved to the lower part of the microscope, and the operator can conveniently observe the cells in each cell dish; the technical problem that cells in an incubator cannot be directly detected in the prior art is solved.

Thirdly, warm water with a certain temperature can be introduced into the heat preservation bag through the hot water temperature control assembly, and water can be changed in the heat preservation bag at intervals, so that the cell sap in the culture dish can keep the temperature within a certain range.

Fourthly, the cell dish cleaning mechanism can clean each cell dish positioned below the cleaning brush, the cell dish can store another type of cells conveniently, manual operation is not needed, great convenience is brought to workers, the rotating shaft can be driven to rotate by the operation of the lifting motor, the rotating shaft can drive the driving wheel to rotate, the driving wheel can drive the driving rod to rotate, the driving rod can drive the sliding block hinged with the driving rod to move along the length direction of the sliding rail, the sliding block can drive the supporting frame to move, the cleaning brush can move to the inside of the cell dish, the rotating shaft can be rotated under the operation of the rotating motor, and the cleaning brush can be driven to rotate by the rotating shaft, so that the cleaning operation of the cell dish is realized.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a top view of the present invention;

FIG. 3 is a cross-sectional view taken along line A-A of FIG. 2;

FIG. 4 is a cross-sectional view of the present invention;

FIG. 5 is an enlarged view of FIG. 4 at B;

FIG. 6 is an enlarged view at C of FIG. 4;

FIG. 7 is a partial cross-sectional view of the first embodiment of the present invention;

fig. 8 is a partial sectional view of the second embodiment of the present invention.

The reference numbers in the figures are:

a base 1, an incubator 2, a sealing cover 21, a handle 22, an ultraviolet germicidal lamp 23, a cell condition observation mechanism 3, an observation cover 31, an operation slot 32, an operation glove 33, a bracket 34, a microscope 35, a liquid inlet pipe 36, a culture dish storage mechanism 4, a rotating motor 41, a bearing plate 42, a limit ring 43, a rotating column 44, a connecting seat 45, a worm 46, a turbine 47, a cell dish washing mechanism 5, a rotating motor 51, a supporting frame 52, a rotating shaft 53, a sliding rail 54, a sliding block 55, a washing pipe 56, a washing nozzle 57, a washing brush 58, a damping mechanism 6, a damping buffer assembly 61, a damping part 62, a mounting column 63, a sliding column 64, a damping spring 65, a cross bar 66, an extension plate 67, a partition vertical plate 68, a buffer block 69, a damping bar 691, a mounting seat 692, a first damping spring 693, a mounting bottom plate 694, a temperature-controlled hot water assembly 7, a heat, liquid feeding pipeline 73, water inlet pipeline 74, backflow pipeline 75, backflow pump 76, backflow corrugated hose 77, liquid inlet pump 78, water inlet corrugated hose 79, anti-shaking assembly 8, second buffer spring 81, side plate 82, lifting assembly 9, rotating shaft 91, driving wheel 92, driving rod 93 and lifting motor 94.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

Referring to fig. 1 to 8, the cell condition monitoring device comprises a base 1, an incubator 2, a cell condition observation mechanism 3, a culture dish storage mechanism 4, a cell dish cleaning mechanism 5 and a damping mechanism 6 arranged at the bottom end inside the incubator 2, wherein the incubator 2 is arranged at the top of the base 1, the top of the incubator 2 is provided with a sealing cover 21 matched with the clamping connection of the sealing cover 21, the top of the sealing cover 21 is provided with a handle 22, the cell condition observation mechanism 3 is arranged at the front part of the incubator 2, one side of the incubator 2 is provided with a hot water temperature control assembly 7, the culture dish storage mechanism 4 is arranged at the top of the damping mechanism 6, the cell dish cleaning mechanism 5 is arranged on the inner wall of the incubator 2, two ultraviolet sterilizing lamps 23 are arranged inside the incubator 2, the two ultraviolet sterilizing lamps 23 are respectively arranged at two sides inside the incubator 2, and the damping mechanism 6 comprises a damping buffer assembly 61, The shock absorption and damping device comprises a horizontally arranged mounting bottom plate 694 and two anti-shaking assemblies 8 which are respectively arranged on two sides of the mounting bottom plate 694, wherein the shock absorption and damping assemblies 61 are arranged at the bottom of the mounting bottom plate 694; warm water with a certain temperature can be introduced into the heat preservation bag 71 through the hot water temperature control assembly 7, water can be changed in the heat preservation bag 71 at intervals, so that the temperature of cell liquid in the culture dish can be kept within a certain range, cells in each culture dish can be observed through the cell condition observation mechanism 3, the culture dish can be observed through the microscope 35 in the operation groove 32 without opening the sealing cover 21, an operator can adjust the microscope 35 through the operation gloves 33, each cell dish positioned below the cleaning brush 58 can be cleaned through the cell dish cleaning mechanism 5, the cell dish can store another type of cells conveniently, manual operation is not needed, great convenience is brought to the operator, and the damping and buffering operation on the heat preservation box 72 can be realized under the action of the damping mechanism 6, avoid incubator 2 to cause the damage to the cell of depositing easily at the in-process that removes to anti-shake subassembly 8 can cushion the swing of mounting plate 694 in the left and right sides direction, avoids mounting plate 694 to take place great rocking and influences the normal condition of depositing the cell.

The shock absorption and buffering assembly 61 comprises two shock absorption parts 62 which are arranged at the bottom of a mounting base plate 694 at intervals and have the same structure, each shock absorption part 62 comprises two vertically arranged mounting columns 63 and two sliding columns 64 which are respectively in sliding fit with the two mounting columns 63, the mounting base plate 694 is arranged at the tops of the two sliding columns 64, shock absorption springs 65 are arranged inside the mounting columns 63, and two ends of each shock absorption spring 65 are respectively connected to the bottom ends inside the mounting columns 63 and the bottoms of the sliding columns 64; the damping and buffering operation of the incubator 72 can be realized under the action of the damping mechanism 6, the damage to the stored cells easily caused in the moving process of the incubator 2 is avoided, the anti-shaking assembly 8 can buffer the swing of the installation bottom plate 694 in the left and right directions, and the influence of the large shaking of the installation bottom plate 694 on the normal state of the stored cells is avoided.

Each damping component 62 further comprises a cross rod 66 and two extending plates 67 arranged at the bottom of the mounting base plate 694 at intervals, the cross rod 66 is horizontally arranged between the two extending plates 67, a partition vertical plate 68 is arranged in the middle of the cross rod 66, two buffer blocks 69 in sliding fit with the cross rod 66 are sleeved on the cross rod 66, each buffer block 69 is provided with a damping rod 691 hinged with the buffer block 69, the side walls of the two mounting columns 63 are provided with mounting seats 692, the two damping rods 691 are hinged with the two mounting seats 692 respectively, two first damping springs 693 are arranged on the cross rod 66 corresponding to each buffer block 69, and the buffer block 69 is arranged between the two first damping springs 693; when the cultivation box 2 jounces during movement, the damping spring 65 can damp the movement of the sliding column 64, and the first damping spring 693 can damp the movement of the damping block 69, thereby reducing the vibration of the mounting base 694 in the vertical direction.

Each anti-shaking assembly 8 comprises a side plate 82 arranged on one side of the mounting bottom plate 694 and two second buffer springs 81 arranged at intervals, and two ends of each second buffer spring 81 are respectively connected to the side wall of the incubator 2 and the side wall of the side plate 82; the second buffer spring 81 can buffer the lateral movement of the mounting base 694.

The culture dish storage mechanism 4 comprises a rotating motor 41, a horizontally arranged bearing disc 42 and a plurality of limiting rings 43 which are circumferentially distributed on the top of the bearing disc 42, the bearing disc 42 is made of heat conducting materials, a vertically arranged rotating column 44 is arranged at the bottom of the bearing disc 42, the bottom end of the rotating column 44 is connected with the top of a mounting bottom plate 694 through a bearing, two connecting seats 45 which are arranged at intervals are arranged at the top of the mounting bottom plate 694, a worm 46 which is rotatably connected with the two connecting seats 45 is arranged between the two connecting seats 45, a turbine 47 which is meshed with the worm 46 is sleeved on the rotating column 44, and the output end of the rotating motor 41 is fixedly connected with one end of the worm 46; deposit mechanism 4 through the culture dish and can make a plurality of cell dishes that are located bear the weight of and coil 42 top rotate, make every cell dish can be located the below of inlet channel 36 in proper order, and make every cell dish can remove the below to microscope 35 in proper order, the worker of being convenient for observes the cell in every cell dish, rotating electrical machines 41 work can drive worm 46 and rotate, make worm 46 can drive the turbine 47 rather than the meshing and take place to rotate, make turbine 47 can drive rotation column 44 and rotate, make and bear dish 42 and rotate along the axis of rotation column 44, make a plurality of cell dish take place to rotate simultaneously.

The cell dish cleaning mechanism 5 comprises a rotating motor 51, a lifting assembly 9, a support frame 52, a rotating shaft 53, a vertically arranged slide rail 54 and a slide block 55 in sliding fit with the slide rail 54, the support frame 52 is arranged on the side wall of the slide block 55, the top end of the rotating shaft 53 can be vertically arranged on the support frame 52 in a rotating manner, the rotating motor 51 is arranged at the top of the support frame 52, the output end of the rotating motor 51 is fixedly connected with the top end of the rotating shaft 53, a cleaning pipeline 56 is arranged on the incubator 2, a cleaning nozzle 57 is arranged at the liquid outlet end of the cleaning pipeline 56, a first electromagnetic valve is arranged on the cleaning pipeline 56, and a cleaning brush 58 is arranged at the bottom end of the rotating shaft 53; can realize carrying out the cleaning operation to every cell dish that is located the cleaning brush 58 below through cell dish wiper mechanism 5, the cell dish of being convenient for is deposited another type of cell, need not artifical manual operation, great convenience has been brought for the worker, can let in the washing liquid to cleaning tube 56 through cleaning tube 56, make cleaning nozzle 57 spout the washing liquid with the form of atomizing, make spun washing liquid can effectively wash the cell dish, make cleaning brush 58 can remove to the inside of cell dish under the effect of lifting unit 9, can make axis of rotation 53 rotate under the work of rotating motor 51, make axis of rotation 53 can drive cleaning brush 58 and rotate, realize the cleaning operation to the cell dish.

The lifting assembly 9 comprises a lifting motor 94 and a rotating shaft 91 which is arranged on the incubator 2 and is horizontally arranged, a driving wheel 92 is sleeved on the rotating shaft 91, a driving rod 93 which is hinged with the driving wheel 92 is arranged on the driving wheel 92, the end part of the driving rod 93 is hinged with the side wall of the sliding block 55, the lifting motor 94 is arranged on the side wall of the incubator 2, and the output end of the lifting motor 94 is fixedly connected with the end part of the rotating shaft 91; the lifting motor 94 is capable of driving the rotating shaft 91 to rotate, so that the rotating shaft 91 can drive the driving wheel 92 to rotate, the driving wheel 92 can drive the driving rod 93 to rotate, the driving rod 93 can drive the sliding block 55 hinged to the driving rod to move along the length direction of the sliding rail 54, the sliding block 55 can drive the supporting frame 52 to move, and the cleaning brush 58 can move to the inside of the cell dish.

The cell condition observation mechanism 3 comprises an observation cover 31 arranged inside the incubator 2, an operation groove 32 is arranged on the observation cover 31, the observation cover 31 is made of transparent materials, two operation gloves 33 symmetrically arranged are arranged at the bottom of the observation cover 31, a support 34 arranged on the inner wall of the incubator 2 is arranged below the observation cover 31, a microscope 35 is arranged on the support 34, a liquid inlet pipeline 36 extending into the incubator 2 is arranged on one side of the incubator 2, and a second electromagnetic valve is arranged on the liquid inlet pipeline 36; can observe the cell in every culture dish through cell situation observation mechanism 3, need not to open sealed lid 21 and can observe the culture dish through microscope 35 in operation groove 32, can add cell culture liquid to the cell dish through inlet channel 36, can make every cell dish remove to the below of inlet channel 36 in proper order under the effect of rotating electrical machines 41, be convenient for add cell culture liquid to the cell dish through inlet channel 36 in, the worker can adjust microscope 35 through operation gloves 33.

The hot water temperature control assembly 7 comprises a heat preservation bag 71 arranged at the bottom of the bearing plate 42 and a heat preservation box 72 arranged on the side wall of the incubator 2, a heater is arranged in the heat preservation box 72, a water inlet pipeline 74 and a backflow pipeline 75 are arranged on the heat preservation bag 71, a liquid feeding pipeline 73 communicated with the interior of the heat preservation box 72 is arranged at the top of the heat preservation box 72, a backflow pump 76 and a liquid inlet pump 78 are arranged below the heat preservation box 72, a water inlet corrugated hose 79 is arranged between the output end of the liquid inlet pump 78 and the water inlet pipeline 74, the input end of the liquid inlet pump 78 is communicated with the bottom of the heat preservation box 72, the output end of the backflow pump 76 is communicated with the upper section of the interior of the heat preservation box 72 through a hose, and a backflow corrugated hose; the warm water with a certain temperature can be introduced into the heat preservation bag 71 through the hot water temperature control assembly 7, and water can be changed in the heat preservation bag 71 at intervals, so that the temperature of cell sap in a culture dish can be kept in a certain range, workers can manually add water into the heat preservation bag 72, the heater can heat the water, the water can be heated to the temperature in the certain range, the water in the heat preservation bag 72 can be pumped out through the working of the liquid inlet pump 78, the pumped water can be conveyed into the heat preservation bag 71 through the water inlet corrugated hose 79, the water in the heat preservation bag 71 can be pumped out through the backflow corrugated hose 77 through the working of the backflow pump 76 at intervals, and the pumped backflow corrugated hose 77 can be conveyed into the heat preservation bag 72 to be heated again.

The working principle of the invention is as follows: the worker can manually open the sealing cover 21 through the handle 22, the worker can place the culture dish in the limiting ring 43, the limiting ring 43 can limit the culture dish, the culture dish is prevented from deviating in the rotating process, warm water with a certain temperature can be introduced into the heat preservation bag 71 through the hot water temperature control assembly 7, water can be exchanged in the heat preservation bag 71 at intervals, so that the cell sap in the culture dish can keep the temperature in a certain range, the worker manually adds water into the heat preservation box 72, the heater can heat the water to the temperature in the certain range, the liquid inlet pump 78 can pump the water in the heat preservation box 72, the pumped water can be conveyed into the heat preservation bag 71 through the water inlet corrugated hose 79, and the reflux pump 76 can pump the water in the heat preservation bag 71 through the reflux corrugated hose 77 at intervals, the pumped backflow corrugated hose 77 can be conveyed into the incubator 72 to be heated again, cells in each culture dish can be observed through the cell condition observation mechanism 3, the culture dishes can be observed through the microscope 35 in the operation groove 32 without opening the sealing cover 21, cell culture fluid can be added into the cell dishes through the fluid inlet pipeline 36, each cell dish can be sequentially moved to the lower part of the fluid inlet pipeline 36 under the action of the rotating motor 41, the cell culture fluid can be conveniently added into the cell dishes through the fluid inlet pipeline 36, a plurality of cell dishes positioned at the top of the bearing plate 42 can be rotated through the culture dish storage mechanism 4, each cell dish can be sequentially positioned below the fluid inlet pipeline 36, each cell dish can be sequentially moved to the lower part of the microscope 35, and workers can conveniently observe the cells in each cell dish, the rotating motor 41 can drive the worm 46 to rotate when working, so that the worm 46 can drive the worm wheel 47 engaged with the worm 46 to rotate, the worm wheel 47 can drive the rotating column 44 to rotate, the bearing disc 42 can rotate along the axis of the rotating column 44, so that a plurality of cell dishes can rotate simultaneously, the cell dish cleaning mechanism 5 can clean each cell dish positioned below the cleaning brush 58, so that the cell dish can store another type of cells, manual operation is not needed, great convenience is brought to workers, cleaning liquid can be introduced into the cleaning pipeline 56 through the cleaning pipeline 56, so that the cleaning liquid can be sprayed out in an atomized mode by the cleaning nozzle 57, the sprayed cleaning liquid can effectively clean the cell dish, the lifting motor 94 can drive the rotating shaft 91 to rotate, and the rotating shaft 91 can drive the driving wheel 92 to rotate, the driving wheel 92 can drive the driving rod 93 to rotate, the driving rod 93 can drive the sliding block 55 hinged with the driving rod 93 to move along the length direction of the sliding rail 54, the sliding block 55 can drive the supporting frame 52 to move, the cleaning brush 58 can move to the inside of the cell dish, the rotating shaft 53 can be rotated under the operation of the rotating motor 51, and the rotating shaft 53 can drive the cleaning brush 58 to rotate, so that the cleaning operation of the cell dish is realized; the damping and buffering operation of the incubator 72 can be realized under the action of the damping mechanism 6, the incubator 2 is prevented from being easily damaged to the stored cells in the moving process, the anti-shaking assembly 8 can buffer the swing of the mounting bottom plate 694 in the left-right direction, the mounting bottom plate 694 is prevented from shaking greatly to influence the normal state of the stored cells, when the incubator 2 is bumped in the moving process, the damping springs 65 can slow down the movement of the sliding columns 64, the first damping springs 693 can buffer the movement of the buffer blocks 69, the vibration of the mounting bottom plate 694 in the vertical direction is reduced, and the second damping springs 81 can buffer the transverse movement of the mounting bottom plate 694.

Claims

1. The temporary cell culture equipment is characterized by comprising a base (1), an incubator (2), a cell condition observation mechanism (3), a culture dish storage mechanism (4), a cell dish cleaning mechanism (5) and a damping mechanism (6) arranged at the bottom end inside the incubator (2), wherein the incubator (2) is arranged at the top of the base (1), the top of the incubator (2) is provided with a sealing cover (21) matched with the incubator in a clamping manner, the top of the sealing cover (21) is provided with a handle (22), the cell condition observation mechanism (3) is arranged at the front part of the incubator (2), one side of the incubator (2) is provided with a hot water temperature control assembly (7), the culture dish storage mechanism (4) is arranged at the top of the damping mechanism (6), and the cell dish cleaning mechanism (5) is arranged on the inner wall of the incubator (2), the inside of incubator (2) is equipped with two sterilamp (23), and two sterilamp (23) set up respectively in the inside both sides of incubator (2), damper (6) are including shock attenuation buffering subassembly (61), mounting plate (694) and two anti-sway subassembly (8) that set up respectively in mounting plate (694) both sides that the level set up, shock attenuation buffering subassembly (61) set up the bottom at mounting plate (694).

2. The cell temporary culture apparatus according to claim 1, wherein the shock absorbing and buffering assembly (61) comprises two shock absorbing parts (62) which are spaced apart from each other and are arranged at the bottom of a mounting base plate (694) and have the same structure, each shock absorbing part (62) comprises two vertically arranged mounting posts (63) and two sliding posts (64) which are slidably engaged with the two mounting posts (63), the mounting base plate (694) is arranged at the top of the two sliding posts (64), the mounting posts (63) are internally provided with shock absorbing springs (65), and the two ends of each shock absorbing spring (65) are respectively connected to the inner bottom ends of the mounting posts (63) and the bottom ends of the sliding posts (64).

3. The cell temporary culture apparatus of claim 2, wherein each of the shock absorbing members (62) further comprises a cross bar (66) and two extension plates (67) spaced apart from each other at the bottom of the mounting base plate (694), the cross bar (66) is horizontally arranged between the two extension plates (67), the middle part of the cross bar (66) is provided with a partition vertical plate (68), the cross rod (66) is sleeved with two buffer blocks (69) in sliding fit with the cross rod, each buffer block (69) is provided with a shock absorption rod (691) hinged with the buffer block, the side walls of the two mounting columns (63) are provided with mounting seats (692), the two shock absorption rods (691) are respectively hinged with the two mounting seats (692), two first buffer springs (693) are arranged on the cross rod (66) corresponding to each buffer block (69), the buffer block (69) is arranged between the two first buffer springs (693).

4. The temporary cell culture apparatus according to claim 1, wherein each of the anti-shaking assemblies (8) comprises a side plate (82) provided at one side of a mounting bottom plate (694) and two second buffer springs (81) provided at intervals, and both ends of each of the second buffer springs (81) are connected to a side wall of the incubator (2) and a side wall of the side plate (82), respectively.

5. The temporary cell culture apparatus according to claim 1, wherein the culture dish storage mechanism (4) comprises a rotary motor (41), a horizontally arranged carrier plate (42) and a plurality of spacing rings (43) circumferentially distributed on the top of the carrier plate (42), the bearing disc (42) is made of heat conducting materials, the bottom of the bearing disc (42) is provided with a vertically arranged rotating column (44), the bottom end of the rotating column (44) is connected with the top of the mounting bottom plate (694) through a bearing, the top of the mounting bottom plate (694) is provided with two connecting seats (45) which are arranged at intervals, a worm (46) which is rotationally connected with the two connecting seats (45) is arranged between the two connecting seats, a turbine wheel (47) meshed with the worm (46) is sleeved on the rotating column (44), the output end of the rotating motor (41) is fixedly connected with one end of the worm (46).

6. The cell temporary culture equipment according to claim 1, wherein the cell dish cleaning mechanism (5) comprises a rotating motor (51), a lifting assembly (9), a support frame (52), a rotating shaft (53), a vertically arranged slide rail (54) and a slide block (55) which is in sliding fit with the slide rail (54), the support frame (52) is arranged on the side wall of the slide block (55), the top end of the rotating shaft (53) can be vertically arranged on the support frame (52) in a rotating manner, the rotating motor (51) is arranged at the top of the support frame (52), the output end of the rotating motor (51) is fixedly connected with the top end of the rotating shaft (53), a cleaning pipeline (56) is arranged on the incubator (2), a cleaning nozzle (57) is arranged at the liquid outlet end of the cleaning pipeline (56), and a first electromagnetic valve is arranged on the cleaning pipeline (56), and a cleaning brush (58) is arranged at the bottom end of the rotating shaft (53).

7. The cell temporary culture equipment according to claim 6, wherein the lifting assembly (9) comprises a lifting motor (94) and a rotating shaft (91) which is arranged on the incubator (2) and is horizontally arranged, a driving wheel (92) is sleeved on the rotating shaft (91), a driving rod (93) which is hinged with the driving wheel (92) is arranged on the driving wheel (92), the end part of the driving rod (93) is hinged with the side wall of the sliding block (55), the lifting motor (94) is arranged on the side wall of the incubator (2), and the output end of the lifting motor (94) is fixedly connected with the end part of the rotating shaft (91).

8. The temporary cell culture equipment according to claim 1, wherein the observation mechanism (3) comprises an observation cover (31) arranged inside the incubator (2), an operation groove (32) is arranged on the observation cover (31), the observation cover (31) is made of transparent material, two operation gloves (33) symmetrically arranged are arranged at the bottom of the observation cover (31), a support (34) arranged on the inner wall of the incubator (2) is arranged below the observation cover (31), a microscope (35) is arranged on the support (34), a liquid inlet pipeline (36) extending to the inside of the incubator (2) is arranged on one side of the incubator (2), and a second electromagnetic valve is arranged on the liquid inlet pipeline (36).

9. The temporary cell culture equipment according to claim 5, wherein the hot water temperature control assembly (7) comprises a heat preservation bag (71) arranged at the bottom of the bearing plate (42) and a heat preservation box (72) arranged on the side wall of the incubator (2), a heater is arranged in the heat preservation box (72), a water inlet pipeline (74) and a return pipeline (75) are arranged on the heat preservation bag (71), a liquid feeding pipeline (73) communicated with the inside of the heat preservation box (72) is arranged at the top of the heat preservation box (72), a return pump (76) and a liquid inlet pump (78) are arranged below the heat preservation box (72), a water inlet corrugated hose (79) is arranged between the output end of the liquid inlet pump (78) and the water inlet pipeline (74), the input end of the liquid inlet pump (78) is communicated with the bottom of the heat preservation box (72), and the output end of the return pump (76) is communicated with the upper section inside the heat preservation box (, and a backflow corrugated hose (77) is arranged between the input end of the backflow pump (76) and the backflow pipeline (75).