CN113322155B

CN113322155B - Incubator for helicobacter pylori

Info

Publication number: CN113322155B
Application number: CN202110596845.1A
Authority: CN
Inventors: 蒋会超
Original assignee: Jinan Qianmai Medical Laboratory Co ltd
Current assignee: Jinan Qianmai Medical Laboratory Co ltd
Priority date: 2021-05-31
Filing date: 2021-05-31
Publication date: 2022-07-22
Anticipated expiration: 2041-05-31
Also published as: CN113322155A

Abstract

The application relates to a culture box for helicobacter pylori. The incubator for helicobacter pylori includes liquid culture case and solid incubator, four gas-supply pipes that set up from top to bottom and set up in the rotation axis of liquid culture case center line, and along the platform of placing that four levels that the length direction of rotation axis equidistant set up. Four gas transmission pipes and the like are horizontally and rotatably arranged around the liquid incubator. And a plurality of air outlet holes are formed in the side wall of each air delivery pipe at intervals. The four placing tables are arranged on the rotating shaft at equal central angles, and the air pipes and the placing tables are sequentially and alternately arranged along the extending direction of the rotating shaft. That is, the gas pipe, the placement table, the gas pipe, and the placement table are arranged in this order in the direction extending along the rotation axis. The placing table is rotated clockwise or counterclockwise in the horizontal direction. Can keep the culture solution in a shaking state and ensure the stability of the liquid environment.

Description

Incubator for helicobacter pylori

Technical Field

The application relates to the field of biology, in particular to a helicobacter pylori incubator.

Background

Helicobacter pylori is an important factor causing gastric cancer. 67% to 80% of gastric ulcers and 95% of duodenal ulcers are caused by helicobacter pylori. The common symptoms of patients with chronic gastritis and peptic ulcer are: after eating, the upper abdomen is full, uncomfortable or painful, often accompanied by other adverse symptoms such as belching, abdominal distension, acid regurgitation, anorexia, etc. Some patients may also have recurrent severe abdominal pain, minor upper gastrointestinal bleeding, etc. Therefore, experts think that people who find helicobacter pylori infection as soon as possible can kill helicobacter pylori with antibiotics timely and effectively, and the method has great significance for preventing and controlling gastric cancer.

Therefore, the culture of helicobacter pylori is of great importance in studying how to eliminate and inhibit helicobacter pylori. However, the growth conditions of helicobacter pylori are severe, and how to strictly control the growth environment of helicobacter pylori is a problem to be solved urgently by the existing helicobacter pylori culture device.

Disclosure of Invention

In view of the above, it is necessary to provide a culture box for helicobacter pylori in order to solve the above-mentioned problems.

An incubator for helicobacter pylori, comprising:

a liquid incubator and a solid incubator which are arranged up and down;

the four gas pipes are horizontally and rotatably arranged around the liquid incubator at equal central angles, and a plurality of gas outlet holes are formed in the side wall of each gas pipe at intervals;

the four horizontal placing tables are arranged at equal intervals along the length direction of the rotating shaft, the four placing tables are arranged at equal central angles on the rotating shaft, and the gas pipes and the placing tables are sequentially and alternately arranged along the extending direction of the rotating shaft;

during operation, the gas-supply pipe is periodic swing, the rotation axis drives four place the platform and rotate towards.

In one embodiment, the swing period of the air conveying pipe is one half of the rotation period of the placing platform, and the placing platform rotates 180 degrees to convert one rotation direction.

In one embodiment, each of the placing stages sequentially places a plurality of culture dishes with sequentially increasing heights in a direction approaching the rotation axis.

In one embodiment, the solid culture box further comprises five micro-oxygen storage tanks, wherein the five micro-oxygen storage tanks are respectively communicated with the four gas conveying pipes and the solid culture box, and the micro-oxygen storage tanks are used for storing mixed gas containing 5% of oxygen, 15% of carbon dioxide and 85% of nitrogen.

In one embodiment, further comprising:

the movable cover body is arranged at the top of the liquid incubator;

the first sealing cover is provided with a first gap and is rotatably sleeved on the rotating shaft; and

the second sealing cover is provided with a second notch, and the second sealing cover is rotatably sleeved on the rotating shaft.

In one embodiment, further comprising:

the first gear rotating drum is coaxially arranged with the rotating shaft, and one end of the first gear rotating drum is provided with the first sealing cover;

the second gear rotating drum is coaxially arranged with the rotating shaft, and one end of the second gear rotating drum is provided with the second sealing cover;

the axis of the driving gear is coaxially arranged with the rotating shaft, and the driving gear is meshed with the second gear rotary drum;

and the axis of the steering gear is coaxially arranged with the rotating shaft, and the steering gear is respectively meshed with the first gear drum and the driving gear.

In one embodiment, the first notch and the second notch are the same shape.

In one embodiment, the first and second indentations are scalloped.

In one embodiment, the device further comprises four first motors, and the first motors are in one-to-one corresponding driving connection with the gas conveying pipes.

In one embodiment, the lifting frame is in transmission connection with the rotating shaft and is used for driving the rotating shaft to move in the vertical direction.

In one embodiment, the device further comprises a second motor for driving the driving gear to rotate.

The utility model provides a helicobacter pylori incubator for, place the bench and can place the culture dish. The air pipe swings left and right in the horizontal direction along the shaft arranged in the incubator. The culture solution can be kept in a shaking state, and the balance of each component of the culture solution is ensured. The top of a culture dish all corresponds a gas-supply pipe, and the venthole sets up down, can guarantee the stability of each culture dish top oxygen concentration. Therefore, the volume of the liquid incubator can be enlarged, and a plurality of culture dishes can be placed. Meanwhile, the placing table is rotated clockwise or counterclockwise in the horizontal direction. Can keep the culture solution in a shaking state and ensure the stability of the liquid environment. And solid culture medium can be placed in the solid culture box to culture the helicobacter pylori. Therefore, the culture conditions of the helicobacter pylori in the liquid culture medium and the solid culture medium can be compared.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a top view of a helicobacter pylori incubator according to an embodiment of the present invention;

FIG. 2 is a sectional view of a helicobacter pylori incubator according to an embodiment of the present application;

FIG. 3 is a sectional view of a helicobacter pylori incubator according to another embodiment of the present application;

FIG. 4 is a schematic view of a first seal cover and a second seal cover in an offset position according to an embodiment of the present application;

FIG. 5 is a schematic view of a first seal cap and a second seal cap in relation to one another according to an embodiment of the present disclosure;

FIG. 6 is a side view of a first gear drum, a second gear drum, a drive gear, and a steering gear provided in accordance with an embodiment of the present application;

fig. 7 is a top view of the first gear drum, the second gear drum, the driving gear and the steering gear provided in the embodiment of the present application.

Description of reference numerals:

incubator 10 for helicobacter pylori

Liquid incubator 100

Solid incubator 200

The air delivery conduit 210

Air outlet 211

Rotating shaft 220

Placing table 222

Petri dish 223

Micro-oxygen storage tank 230

Movable cover 240

First sealing cover 251

Second seal cover 252

First gear drum 261

Second gear drum 262

Drive gear 270

Steering gear 280

First electric machine 291

Second electric machine 292

A crane 300.

Detailed Description

Reference will now be made in detail to embodiments of the present application, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative and intended to be used for explaining the present application and should not be construed as limiting the present application.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "clockwise," "counterclockwise," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, unless otherwise specified, "a plurality" means two or more unless explicitly defined otherwise.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral connections; can be mechanically or electrically connected; the two elements can be directly connected with each other or indirectly connected with each other through an intermediate medium, and the two elements can be communicated with each other. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to specific situations.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact of the first and second features, or may comprise contact of the first and second features not directly contact but through another feature in between. Also, a first feature being "on," "above" and "over" a second feature includes the first feature being directly on and obliquely above the second feature, or simply means that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Referring to fig. 1 and 2, an exemplary embodiment of the present disclosure provides an incubator 10 for helicobacter pylori. The incubator 10 for helicobacter pylori includes a liquid incubator 100 and a solid incubator 200 which are vertically disposed, four gas supply pipes 210, a rotation shaft 220 disposed on a center line of the liquid incubator 100, and four horizontally disposed standing tables 222 which are disposed at equal intervals along a length direction of the rotation shaft 220. Four air pipes 210 are horizontally and rotatably arranged around the liquid incubator 100 at equal central angles. A plurality of air outlet holes 211 are formed at intervals in the side wall of each air pipe 210. Four of the placement tables 222 are disposed on the rotating shaft 220 at equal central angles, and the air pipes 210 and the placement tables 222 are alternately disposed in this order in the direction in which the rotating shaft 220 extends. That is, the air pipe 210, the placement table 222, the air pipe 210, and the placement table 222 are arranged in this order in the direction extending along the rotation axis 220. When the incubator works, the air conveying pipe 210 swings left and right in the horizontal direction along the shaft arranged on the incubator. The air outlet 211 is arranged downward. Meanwhile, a culture dish 223 may be placed on the placing table 222. The culture dish 223 can be used for culturing helicobacter pylori by using liquid culture medium. The placing table 222 is rotated in a horizontal direction in a clockwise or counterclockwise direction. The gas pipes 210 swing left and right periodically, the rotating shaft 220 drives the four placing tables 222 to rotate, and the swinging angular speed of the gas pipes 210 is the same as the rotating angular speed of the placing tables 222.

When helicobacter pylori is cultured using a liquid medium, growth in the liquid medium is more difficult because it is more difficult to ensure the microaerophilic environment necessary for the bacterial cells and the stabilization of nutrients, and the continuous diffusion of harmful products, within the liquid. Therefore, in liquid culture, it is necessary to try to constantly shake the culture solution to overcome the above-mentioned disadvantages. By rotating the placing table 222 in the horizontal direction clockwise or counterclockwise, the culture solution can be kept in a shaken state.

In addition, the stable growth of the helicobacter pylori needs to depend on a microenvironment containing 5% -8% of oxygen, so that the helicobacter pylori can not grow in the atmosphere and an absolute anaerobic environment. That is, the growth environment of helicobacter pylori is very strict. Too high or too low oxygen concentration is not suitable for cultivation. In order to ensure the accuracy of oxygen content, the traditional incubator can only be provided with a culture space with a smaller volume. If the culture space is too large, the airtightness and gas fluidity of the apparatus easily cause variation in oxygen content.

According to the technical scheme provided by the scheme, a culture dish 223 can be placed on the placing table 222. The air pipe 210 swings left and right in the horizontal direction along the axis of the incubator. The culture solution can be kept in a shaking state, and the balance of all components of the culture solution is ensured. The upper part of one culture dish 223 corresponds to one gas pipe 210, and the gas outlet 211 is arranged downwards, so that the stability of the oxygen concentration above each culture dish 223 can be ensured. Therefore, the volume of the liquid culture tank 100 can be increased, and a plurality of culture dishes 223 can be placed. Meanwhile, the placing table 222 is rotated in a horizontal direction clockwise or counterclockwise. Can keep the culture solution in a shaking state and ensure the stability of the liquid environment. The solid culture box 200 can be filled with solid culture medium to culture helicobacter pylori. Therefore, the culture conditions of the helicobacter pylori in the liquid culture medium and the solid culture medium can be compared.

In one embodiment, the swing period of the air pipes is half of the rotation period of the placing platform, and the placing platform rotates 180 degrees to convert one rotation direction. That is to say, the air conveying pipe swings left and right once, and the placing platform rotates for a half circle. The placing platform and the air conveying pipe rotate at the same angular speed. The direction of the placing table is rotated once every half turn, the flowing mixing of the liquid culture solution can be promoted, and the uniformity of the culture solution is improved.

In one embodiment, each of the placing stages sequentially places a plurality of culture dishes with sequentially increasing heights in a direction approaching the rotation axis. That is, the petri dishes near the rotating shaft are closer in height to their corresponding gas pipes. Because the linear velocity of the gas pipe close to one end of the rotating shaft is large, the linear velocity of the ejected gas is also large, the diffusion speed is high, and the concentration is reduced quickly. Through making the culture dish that is close to the rotation axis more be close to its gas-supply pipe that corresponds in the height, can give the culture dish air feed when gas concentration has not reduced very much yet, effectively improve all culture dishes place gaseous homogeneity of environment.

In one embodiment, the incubator 10 for helicobacter pylori further includes five micro-oxygen storage tanks 230, and the five micro-oxygen storage tanks 230 are respectively connected to the four gas pipes 210. And the solid incubator 200, the micro-oxygen storage tank 230 is used for storing mixed gas containing 5% of oxygen, 15% of carbon dioxide and 85% of nitrogen.

Referring to fig. 3 to 5, in one embodiment, the culture tank 10 for helicobacter pylori further includes a movable cover 240, a first sealing cover 251 and a second sealing cover 252, wherein the movable cover 240 is disposed on the top of the liquid culture tank 100. The first sealing cover 251 is provided with a first gap, and the first sealing cover 251 is rotatably sleeved on the rotating shaft 220. The second sealing cover 252 is provided with a second gap, and the second sealing cover 252 is rotatably sleeved on the rotating shaft 220. When the culture is performed normally, the first notch and the second notch are in a staggered state. The first sealing cover 251 and the second sealing cover 252 thus function in combination to seal the liquid culture tank 100. When the culture dish 223 on the uppermost placing table 222 needs to be extracted, the first sealing cover 251 and the second sealing cover 252 may be rotated to make the first notch and the second notch opposite to each other, and the rotating shaft is lifted upwards at this time to make the uppermost placing table 222 pass through, and the placing table 222 is stopped between the first sealing cover 251, the second sealing cover 252 and the movable cover 240. The first and second notches are then misaligned to complete the seal of the remaining culture dish 223. At this time, the movable cover 240 is opened, and the culture dish 223 staying between the first and second sealing caps 251 and 252 and the movable cover 240 can be taken out. By providing the first and second sealing caps 251 and 252, it is possible to prevent the culture dishes 223 taken out later from being affected by the non-sealed state when the culture dishes 223 are taken out in sequence.

Referring to fig. 6-7, in one embodiment, the helicobacter pylori incubator 10 further includes a first gear drum 261, a second gear drum 262, a drive gear 270, and a steering gear 280. The first gear drum 261 is disposed coaxially with the rotary shaft 220, and one end of the first gear drum 261 is disposed with the first seal cover 251. A second gear rotating cylinder 262 is provided coaxially with the rotary shaft 220, and one end of the second gear rotating cylinder 262 is provided with the second seal cover 252. The axis of the driving gear 270 is coaxially disposed with the rotary shaft 220, and the driving gear 270 is engaged with the second gear rotary drum 262. The axis of the steering gear 280 is disposed coaxially with the rotary shaft 220, and the steering gear 280 is engaged with the first gear drum 261 and the drive gear 270, respectively. Therefore, when the driving gear 270 rotates counterclockwise, the first gear drum 261 rotates clockwise, the steering gear 280 rotates clockwise, and the second gear drum 262 rotates counterclockwise by the steering gear 280. Thus, the first gear drum 261 and the second gear drum 262 are rotated in opposite directions. By rotating the driving gear 270, the first sealing cover 251 and the second sealing cover 252 can be rotated, and the first notch and the second notch can be opposite or offset.

In one embodiment, the first notch and the second notch are the same shape.

In one embodiment, the first and second indentations are scalloped.

In one embodiment, the air pipe system further comprises four first motors 291, and the first motors 291 are in one-to-one driving connection with the air pipes 210. The first motor 291 is used for driving the air pipe 210 to swing in the horizontal direction.

In one embodiment, the lifting frame 300 is further included, the lifting frame 300 is in transmission connection with the rotating shaft 220, and the lifting frame 300 is used for driving the rotating shaft 220 to move in the vertical direction.

In one embodiment, a second motor 292 is also included for driving rotation of the drive gear 270.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is specific and detailed, but not to be understood as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, and these are all within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. An incubator for helicobacter pylori, comprising:

a liquid incubator and a solid incubator which are arranged up and down;

the culture dishes are placed on the placing table, one gas pipe is corresponding to each upper part of one culture dish, and the gas outlet holes are arranged downwards, so that the stability of the oxygen concentration above each culture dish can be ensured;

when the device works, the gas conveying pipes periodically swing left and right, and the rotating shaft drives the four placing tables to rotate; the placing table rotates 180 degrees to convert the direction of one rotation.

2. The incubator as claimed in claim 1, wherein the period of oscillation of the gas pipe is half of the period of rotation of the placement table.

3. The incubator according to claim 2, wherein a plurality of the culture dishes having successively increasing heights are successively placed on each of the placement stages in a direction approaching the rotation axis.

4. The incubator according to claim 1, further comprising five micro-oxygen storage tanks, wherein the five micro-oxygen storage tanks are respectively communicated with the four gas pipes and the solid incubator, and the micro-oxygen storage tanks are used for storing a mixed gas containing 5% of oxygen, 15% of carbon dioxide and 85% of nitrogen.

5. The incubator for helicobacter pylori according to claim 1, further comprising:

the movable cover body is arranged at the top of the liquid incubator;

6. The incubator for helicobacter pylori according to claim 5, further comprising:

7. The incubator according to claim 5, wherein the first notch and the second notch have the same shape.

8. The incubator as recited in claim 7, wherein said first notch and said second notch are fan-shaped.

9. The incubator according to claim 1, further comprising four first motors, wherein the first motors are in one-to-one driving connection with the gas transmission pipes.

10. The incubator for helicobacter pylori according to claim 1, wherein the incubator further comprises a lid for closing the lid,

the lifting frame is in transmission connection with the rotating shaft and is used for driving the rotating shaft to move in the vertical direction.