CN116656496B - Marine phage culture device - Google Patents
Marine phage culture device Download PDFInfo
- Publication number
- CN116656496B CN116656496B CN202310935153.4A CN202310935153A CN116656496B CN 116656496 B CN116656496 B CN 116656496B CN 202310935153 A CN202310935153 A CN 202310935153A CN 116656496 B CN116656496 B CN 116656496B
- Authority
- CN
- China
- Prior art keywords
- incubator
- fixedly connected
- box
- rod
- threaded rod
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000002093 peripheral effect Effects 0.000 claims abstract description 9
- 230000007246 mechanism Effects 0.000 claims description 10
- 230000005540 biological transmission Effects 0.000 claims description 5
- 229920005549 butyl rubber Polymers 0.000 claims description 5
- 230000000149 penetrating effect Effects 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 3
- 238000011160 research Methods 0.000 description 6
- 238000000151 deposition Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application relates to a marine phage culture device, which belongs to the technical field of microorganism culture and comprises a device table and an incubator fixed above the device table, wherein the upper end of the device table is fixedly connected with an observation box communicated with the right end of the incubator, a storage tree is arranged in the incubator and comprises a base fixed on the inner bottom wall of the incubator, the base is of an n-type in side view, the upper end of the base is connected with the inner top wall of the incubator in a common rotation way, a plurality of layers of storage platforms are vertically and uniformly distributed on the peripheral surface of the rotary rod, each storage platform is composed of four pairs of storage boxes fixedly connected on the peripheral surface of the rotary rod in a uniform circumference manner, and a first motor for driving the rotary rod to rotate is fixedly arranged at the upper end of the incubator. The application can automatically select, take out and store the marine phage culture dish in the incubator, and ensure that the culture environment is not influenced by the outside.
Description
Technical Field
The application relates to the technical field of microorganism culture, in particular to a marine phage culture device.
Background
Marine phage is the most abundant microorganism in the marine ecosystem, has important effects on the flow of marine ecosystem substances and energy and the maintenance of community diversity, and becomes a research hotspot of marine ecology, but the whole research of the marine phage is still in a preliminary stage. The current marine phage research experiment mainly researches biological characteristics, starts from two aspects of genomics and proteomics, explores the attribute and genetic correlation of the marine phage, and provides basis for the research of marine phage in the future.
In marine phage research experiments, phage culture work needs to be carried out, the incubator needs to be used for constructing constant temperature environment for phage culture in the culture process, the quantity of culture dishes used for common culture is more, and because of the difference of phage types and initial culture time, serial numbers and labels need to be carried out on all culture dishes, thereby observe the culture condition after a certain time in the incubator, but most of existing incubators are single-structure cases, the culture dishes on the internal storage partition plate of the existing incubators are placed randomly and are not easy to be stored for classification time, and when the culture dishes are taken out, the incubator needs to be opened for searching for a long time, the temperature change of the incubator is very easy to be caused for long time searching, the culture of other phages is influenced, and if the existing incubators are not in a sterile environment, external viruses and bacteria are also very easy to enter the incubator, so that phage culture fails.
To solve the above problems, we propose a marine phage culture apparatus.
Disclosure of Invention
The application aims to solve the problems in the background art and provides a marine phage culture device.
In order to achieve the above purpose, the present application adopts the following technical scheme: the utility model provides a marine phage culture device, includes the device platform and fixes the incubator above it, device platform upper end fixedly connected with incubator right-hand member intercommunication's observation box, be equipped with in the incubator and deposit the tree, deposit the tree including fixing the base on the inner diapire of incubator, the base looks looking at and is n type and upper end and the inner roof of incubator between rotate together and be connected with the rotary rod, vertically evenly arranged multilayer storage platform on the rotary rod periphery, each storage platform comprises four pairs of even circumference storage boxes fixedly connected with on the rotary rod periphery, incubator upper end fixed mounting has the first motor of drive rotary rod pivoted;
a first threaded rod, a second threaded rod, a first sliding rod and a second sliding rod are arranged between the upper inner wall and the lower inner wall of the incubator, a first lifting block is sleeved between the first threaded rod and the first sliding rod, a second lifting block is sleeved between the second threaded rod and the second sliding rod, and a taking and placing mechanism is arranged between the first lifting block and the second lifting block at equal heights;
the picking and placing mechanism comprises a telescopic cylinder fixedly installed at the center of the first lifting block, the telescopic end of the telescopic cylinder is arranged towards the second lifting block and is fixedly connected with an end plate, the right end of the end plate is fixedly connected with two movable rods which are distributed up and down, the right end of the second lifting block is fixedly connected with a fixed plate, and the right end of the movable rod is positioned above the fixed plate and is fixedly connected with a clamping box.
In the marine phage culture device, four circumferentially distributed clamping blocks are arranged in the clamping box, and springs are fixedly connected between the clamping blocks and the inner peripheral wall of the clamping box.
In the above-mentioned marine phage culture device, both ends are connected with inner wall rotation about the incubator about first threaded rod and the second threaded rod respectively, and both upper ends all extend to the incubator top and fixedly sleeve over first bevel gear, two all have the second bevel gear in perpendicular meshing between the first bevel gear, two the common fixedly connected with transfer line in second bevel gear axle center position is located left first bevel gear left side perpendicular meshing has third bevel gear, incubator upper end fixed mounting has the second motor of drive third bevel gear pivoted.
In the marine phage culture device, two vertical plates which are arranged at intervals around are fixedly connected to the inner bottom wall of the incubator at the left side of the base, a third threaded rod and a third sliding rod are respectively arranged between the right end of the vertical plate and the right inner wall of the observation box, sliding blocks are respectively sleeved on the third threaded rod and the third sliding rod, a dish-shaped movable box is fixedly connected between the sliding blocks, and a third pushing opening is formed in the central position of the lower end of the movable box.
In the marine phage culture device, two ends of the third threaded rod are respectively connected with the vertical plate and the inner wall on the right side of the observation box in a rotating mode, a third motor for driving the third threaded rod to rotate is fixedly arranged at the right end of the observation box, and a taking and placing opening is formed in the right end of the observation box and above the third motor.
In the marine phage culture device, the operation ports are formed in the front wall and the rear wall of the observation box, butyl rubber gloves are fixedly connected in the two operation ports, an observation table is fixedly connected on the inner top wall of the observation box, a microscope is fixedly arranged at the upper end of the observation box, and the eyepiece end of the microscope extends to the upper side of the observation table.
In the above-mentioned marine phage culture device, the case is dish form and the lower extreme central point that the opening is upwards put and has offered first mouth of pushing away, the centre gripping box is dish form and the upper end central point that the opening is downwards put and has offered the second mouth of pushing away, second mouth top fixed mounting has the second electric putter that pushes away.
In the above-mentioned marine phage culture device, two the movable rod right-hand member all slides and runs through the fixed plate setting, is located the below the movable rod right-hand member fixedly connected with installation piece, fixedly connected with electric putter on the installation piece.
Compared with the prior art, the marine phage culture device has the advantages that:
through setting up to deposit the tree and get and put the mechanism, when need carry out the take out the work of marine phage culture dish, need not to open the chamber door for a long time and look for, only need know its position of depositing on the tree after, utilize to get the upward shift and the lateral shifting of putting the mechanism, can move to this culture dish top, move it to the observation incasement voluntarily, can directly carry out confined microscopic observation work as required in the observation incasement, or directly take out it, and when carrying out single depositing, only need put into the culture dish at the observation case, alright be automatic deposit its corresponding position on depositing the tree, take out and deposit the work when having simplified marine phage cultivates greatly, guarantee that its culture environment does not receive external influence.
Drawings
FIG. 1 is a perspective view of a marine phage culture apparatus according to the present application;
FIG. 2 is an enlarged schematic view of the structure of FIG. 1A;
FIG. 3 is a perspective view of the front face of a marine phage culture device according to the present application;
FIG. 4 is an enlarged schematic view of the structure of FIG. 3B;
FIG. 5 is a perspective view of a portion of a top view of a storage tree in a marine phage culture apparatus according to the present application;
FIG. 6 is a perspective view of a part of the structure of the picking and placing mechanism in the marine phage culture device.
In the figure: 1. a device table; 2. an incubator; 3. an observation box; 4. a first motor; 5. a rotating rod; 6. a base; 7. a storage box; 8. a first push port; 9. a first threaded rod; 10. a first slide bar; 11. a first lifting block; 12. a second threaded rod; 13. a second slide bar; 14. a second lifting block; 15. a telescopic cylinder; 16. an end plate; 17. a movable rod; 18. a fixing plate; 19. a clamping box; 20. a mounting block; 21. a first electric push rod; 22. clamping blocks; 23. a second push port; 24. a second electric push rod; 25. a first bevel gear; 26. a second bevel gear; 27. a transmission rod; 28. a third bevel gear; 29. a second motor; 30. a third threaded rod; 31. a third motor; 32. a moving case; 33. an observation stand; 34. a microscope; 35. butyl rubber gloves; 36. and taking and placing the mouth.
Detailed Description
The following examples are for illustrative purposes only and are not intended to limit the scope of the application.
Example 1
Referring to fig. 1 to 6, a marine phage culture apparatus comprises an apparatus table 1 and an incubator 2 fixed above the apparatus table, wherein the front end of the incubator 2 is provided with a box door, and the left end of the incubator 2 is provided with an observation glass. The device platform 1 upper end fixedly connected with incubator 2 right-hand member intercommunication's observation box 3 is equipped with in the incubator 2 and deposits the tree, deposit the tree including fixing the base 6 on the diapire in the incubator 2, the base 6 is looking sideways and is "n" and rotate jointly between upper end and the inner roof of incubator 2 and be connected with rotary rod 5, vertical even multilayer storage platform of having arranged on the rotary rod 5 outer peripheral face, each storage platform comprises four pairs of even circumference fixedly connected case 7 on rotary rod 5 outer peripheral face, the quantity of every layer case 7 is eight, can deposit eight culture dishes, the quantity of depositing of culture dish has been guaranteed. The incubator 2 upper end fixed mounting has the first motor 4 of drive rotary rod 5 pivoted, and first motor 4 can drive and deposit the tree and rotate, makes each case 7 at incubator 2 internal rotation, only need to carry out the serial number to each position case 7, can follow the light each case 7 and its inside culture dish of seeing of every on depositing the tree of sight glass.
The storage box 7 is dish-shaped with an upward opening, the center position of the lower end of the storage box is provided with a first push opening 8, a first threaded rod 9, a second threaded rod 12, a first slide rod 10 and a second slide rod 13 are arranged between the upper inner wall and the lower inner wall of the incubator 2, the first threaded rod 9, the second threaded rod 12, the first slide rod 10 and the second slide rod 13 are distributed in a rectangular shape, and the upper end and the lower end of the first slide rod 10 and the lower end of the second slide rod 13 are respectively fixedly connected with the upper inner side and the lower inner side of the incubator 2. The first lifting block 11 is sleeved between the first threaded rod 9 and the first sliding rod 10, the second threaded rod 12 and the second sliding rod 13 are sleeved with the second lifting block 14, the first threaded rod 9 and the second threaded rod 12 are respectively in threaded connection with the first lifting block 11 and the second lifting block 14, the upper end and the lower end of the first threaded rod 9 and the lower end of the second threaded rod 12 are respectively in rotary connection with the upper inner wall and the lower inner wall of the incubator 2, the upper ends of the first threaded rod 9 and the second threaded rod 12 extend to the upper side of the incubator 2 and are fixedly sleeved with the first bevel gear 25, the second bevel gear 26 is vertically meshed between the two first bevel gears 25, the transmission rod 27 is fixedly connected to the axial center position of the two second bevel gears 26, the third bevel gear 28 is vertically meshed to the left side of the first bevel gear 25 positioned on the left side, the second motor 29 is fixedly arranged to drive the third bevel gear 28 to rotate, the second motor 29 can directly drive the first threaded rod 9 and the second threaded rod 12 to synchronously rotate through the transmission of the first bevel gear 25, the second bevel gear 26, the third bevel gear 28 and the transmission rod 27, and the first lifting block 11 and the second lifting block 14 can be synchronously driven.
The first lifting block 11 and the second lifting block 14 are arranged at equal heights, a picking and placing mechanism is arranged between the first lifting block 11 and the second lifting block 14, the picking and placing mechanism comprises a telescopic cylinder 15 fixedly installed at the center position of the first lifting block 11, the telescopic end of the telescopic cylinder 15 faces the second lifting block 14 and is fixedly connected with an end plate 16, the right end of the end plate 16 is fixedly connected with two movable rods 17 which are distributed up and down, the right end of the second lifting block 14 is fixedly connected with a fixed plate 18, the right ends of the two movable rods 17 are all arranged in a sliding manner and penetrate through the fixed plate 18, the right end of the movable rod 17 located above is fixedly connected with a clamping box 19, the right end of the movable rod 17 located below is fixedly connected with a mounting block 20, four circumferentially distributed clamping blocks 22 are arranged in the clamping box 19, springs are fixedly connected between the clamping blocks 22 and the inner peripheral wall of the clamping box 19 together, the mounting block 20 is fixedly connected with a first electric push rod 21, the clamping box 19 is in a dish shape with a downward opening, the center position of the upper end is provided with a second push opening 23, and the second electric push opening 24 is fixedly installed above the second push opening 23. The telescopic cylinder 15 can drive the clamping box 19 and the first electric push rod 21 to transversely move through the two movable rods 17, so that the clamping box 19 and the first electric push rod 21 can transversely move at the upper and lower positions of the two leftmost storage boxes 7 at the position of the corresponding storage platform by being matched with the lifting of the first lifting block 11 and the second lifting block 14. When the clamping box 19 and the first electric push rod 21 are located at the included angle of the storage platform, the clamping box can move up and down along with the first lifting block 11 and the second lifting block 14.
Specifically, through the cooperation work of the second motor 29 and the telescopic cylinder 15, the clamping box 19 and the first electric push rod 21 can be moved to the position of any storage box 7 at the left end of the storage tree, and when the position of the clamping box is corresponding to the position of the storage box 7 up and down, the first electric push rod 21 can jack up the culture dish upwards through the first pushing opening 8 of the storage box 7 corresponding to the upper part, so that the upper end of the culture dish is inserted into the clamping box 19 and is elastically clamped by the four clamping blocks 22 in the clamping box 19, and then the first motor 4 drives the storage tree to rotate for fifteen degrees, so that the clamping box 19 and the first electric push rod 21 are positioned at the included angle position of the storage platform, and the clamped culture dish can be driven to move downwards.
The bottom wall in the incubator 2 is fixedly connected with two vertical plates which are arranged at intervals from front to back at the left side of the base 6, a third threaded rod 30 and a third sliding rod are respectively arranged between the right ends of the two vertical plates and the right inner wall of the observation box 3, sliding blocks are respectively sleeved on the third threaded rod 30 and the third sliding rod, and two ends of the third sliding rod are rotationally connected with the corresponding vertical plates and the right inner wall of the observation box 3. The movable box 32 is fixedly connected between the two sliding blocks, a third pushing opening is formed in the center position of the lower end of the movable box 32, two ends of a third threaded rod 30 are respectively connected with a vertical plate and the inner wall on the right side of the observation box 3 in a rotating mode, a third motor 31 for driving the third threaded rod 30 to rotate is fixedly arranged at the right end of the observation box 3, a picking and placing opening 36 is formed in the position above the third motor 31, and the third motor 31 can drive the movable box 32 to move through the third threaded rod 30.
Specifically, when the required culture dish needs to be taken out, the position of the culture dish is observed from the observation window, the taking and placing mechanism is moved to the same position through the second motor 29, the culture dish is rotated to the left side through the first motor 4, the clamping box 19 and the first electric push rod 21 are moved to the upper and lower positions through the telescopic cylinder 15, the culture dish can be pushed into the clamping box 19, the culture dish is driven to move downwards to the position between the two vertical plates, the moving box 32 is driven to move leftwards through the third motor 31 at the moment, the culture dish is moved to the position between the clamping box 19 and the first electric push rod 21, the second electric push rod 24 pushes the culture dish downwards through the second push opening 23, the culture dish can be dropped into the moving box 32, the taking and placing opening 36 can be opened along with the movement of the moving box 32 into the observation box 3, and the culture dish is taken out. Without opening the incubator 2 for a long time to search. The corresponding culture dish can be taken out rapidly and accurately.
When the culture dish is required to be placed in the culture dish independently, the culture dish is placed in the movable box 32 from the taking and placing opening 36, then is moved to the left between the clamping box 19 and the first electric push rod 21, is pushed into the clamping box 19 by the first electric push rod 21, accordingly moves to the height of a designated storage platform along with the upper movement of the clamping box 19 and the first electric push rod 21, is matched with the rotation of a storage tree, moves to the upper part of the corresponding storage box 7, is pushed into the corresponding storage box 7 downwards by the second electric push rod 24, is automatically stored, does not need to manually open a box door for a long time to store, and ensures the environment of the incubator 2.
The operation ports are formed in the front wall and the rear wall of the observation box 3, the butyl rubber gloves 35 are fixedly connected in the two operation ports, the observation table 33 is fixedly connected to the inner top wall of the observation box 3, the microscope 34 is fixedly arranged at the upper end of the observation box 3, the eyepiece end of the microscope 34 extends to the upper portion of the observation table 33, when the culture dish is required to be temporarily observed for the marine phage in the culture dish, the culture dish is moved into the observation box 3 in the same way as the above-mentioned taking-out mode, the culture dish is only required to be taken down by the two butyl rubber gloves 35 and opened, and is placed on the observation table 33, so that the observation can be directly performed by using the microscope 34, the culture dish is not required to be taken out when the observation is convenient, and the observation environment is not influenced by the outside.
The foregoing description of the preferred embodiments of the application is not intended to limit the application to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the application are intended to be included within the scope of the application.
Claims (5)
1. The utility model provides a marine phage culture device, includes device table (1) and incubator (2) of fixing above it, device table (1) upper end fixedly connected with incubator (2) right-hand member intercommunication observe case (3), a serial communication port, be equipped with in incubator (2) and deposit the tree, it includes base (6) of fixing on the inner diapire of incubator (2), base (6) are the side view and are the rotation connection in common between upper end and the inner roof of incubator (2) rotary rod (5), multilayer storage platform has vertically evenly been arranged on rotary rod (5) outer peripheral face, each storage platform comprises four pairs of even circumference fixedly connected with case (7) on rotary rod (5) outer peripheral face, incubator (2) upper end fixedly has first motor (4) of drive rotary rod (5) pivoted;
a first threaded rod (9), a second threaded rod (12), a first sliding rod (10) and a second sliding rod (13) are arranged between the upper inner wall and the lower inner wall of the incubator (2), a first lifting block (11) is sleeved between the first threaded rod (9) and the first sliding rod (10), a second lifting block (14) is sleeved between the second threaded rod (12) and the second sliding rod (13), and the first lifting block (11) and the second lifting block (14) are arranged at equal heights and are provided with a picking and placing mechanism;
the picking and placing mechanism comprises a telescopic cylinder (15) fixedly arranged at the center of a first lifting block (11), the telescopic end of the telescopic cylinder (15) is arranged towards a second lifting block (14) and is fixedly connected with an end plate (16), the right end of the end plate (16) is fixedly connected with two movable rods (17) which are distributed up and down, the right end of the second lifting block (14) is fixedly connected with a fixed plate (18), and the right end of the movable rod (17) above is fixedly connected with a clamping box (19);
two vertical plates which are arranged at intervals from front to back are fixedly connected to the inner bottom wall of the incubator (2) at the left side of the base (6), a third threaded rod (30) and a third sliding rod are respectively arranged between the right end of the vertical plate and the right inner wall of the observation box (3), sliding blocks are respectively sleeved on the third threaded rod (30) and the third sliding rod, a dish-shaped movable box (32) is fixedly connected between the two sliding blocks together, and a third pushing opening is formed in the center position of the lower end of the movable box (32);
the storage box (7) is dish-shaped with an upward opening, a first pushing opening (8) is formed in the center of the lower end of the storage box, the clamping box (19) is dish-shaped with a downward opening, a second pushing opening (23) is formed in the center of the upper end of the clamping box, and a second electric push rod (24) is fixedly arranged above the second pushing opening (23);
the right ends of the two movable rods (17) are arranged in a sliding penetrating mode through the fixed plate (18), the right ends of the movable rods (17) located below are fixedly connected with mounting blocks (20), and the mounting blocks (20) are fixedly connected with first electric push rods (21).
2. The marine phage culture device according to claim 1, wherein four circumferentially distributed clamping blocks (22) are arranged in the clamping box (19), and springs are fixedly connected between the clamping blocks (22) and the inner peripheral wall of the clamping box (19).
3. The marine phage culture device according to claim 1, wherein the upper end and the lower end of the first threaded rod (9) and the second threaded rod (12) are respectively connected with the upper inner wall and the lower inner wall of the incubator (2) in a rotating way, the upper ends of the first threaded rod and the second threaded rod extend to the upper part of the incubator (2) and are fixedly sleeved with a first bevel gear (25), a second bevel gear (26) is vertically meshed between the two first bevel gears (25), a transmission rod (27) is fixedly connected to the axial center position of the two second bevel gears (26), a third bevel gear (28) is vertically meshed to the left side of the first bevel gear (25) positioned on the left side, and a second motor (29) for driving the third bevel gear (28) to rotate is fixedly arranged at the upper end of the incubator (2).
4. The marine phage culture device according to claim 1, wherein two ends of the third threaded rod (30) are respectively connected with a vertical plate and the right inner wall of the observation box (3) in a rotating mode, a third motor (31) for driving the third threaded rod (30) to rotate is fixedly arranged at the right end of the observation box (3), and a picking and placing opening (36) is formed in the right end of the observation box (3) and above the third motor (31).
5. The marine phage culture device according to claim 1, wherein operation ports are formed in the front wall and the rear wall of the observation box (3), butyl rubber gloves (35) are fixedly connected in the two operation ports, an observation table (33) is fixedly connected on the inner top wall of the observation box (3), a microscope (34) is fixedly arranged at the upper end of the observation box (3), and the eyepiece end of the microscope (34) extends to the upper portion of the observation table (33).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202310935153.4A CN116656496B (en) | 2023-07-28 | 2023-07-28 | Marine phage culture device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202310935153.4A CN116656496B (en) | 2023-07-28 | 2023-07-28 | Marine phage culture device |
Publications (2)
Publication Number | Publication Date |
---|---|
CN116656496A CN116656496A (en) | 2023-08-29 |
CN116656496B true CN116656496B (en) | 2023-10-27 |
Family
ID=87715647
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202310935153.4A Active CN116656496B (en) | 2023-07-28 | 2023-07-28 | Marine phage culture device |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN116656496B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117343825B (en) * | 2023-12-05 | 2024-03-08 | 滨州市鑫荣生物肥业有限公司 | Marine microorganism strain storage device |
CN118496985B (en) * | 2024-07-17 | 2024-10-15 | 中国水产科学研究院黄海水产研究所 | Shellfish pathogenic bacteria phage culture device |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013129558A1 (en) * | 2012-02-29 | 2013-09-06 | 株式会社ジェイテック | Cell culture device having culture medium replacement function |
CN105494219A (en) * | 2015-12-14 | 2016-04-20 | 中国水产科学研究院黄海水产研究所 | Aquaculture pond with vortex sewage discharge performance |
CN210458219U (en) * | 2019-07-19 | 2020-05-05 | 无锡菩禾生物医药技术有限公司 | Multi-layer cell culture box easy to take and place bottom culture dish |
CN111454836A (en) * | 2020-04-14 | 2020-07-28 | 青岛农业大学 | Marine bacteria culture device |
CN211631428U (en) * | 2019-12-30 | 2020-10-09 | 广西华崧生物防控科技有限公司 | Box-type culture dish for culturing entomopathogenic nematodes |
CN113564047A (en) * | 2021-08-09 | 2021-10-29 | 董玉娟 | Culture dish for biological stem cell culture and matched incubator thereof |
CN114456913A (en) * | 2022-02-22 | 2022-05-10 | 宣卫东 | Cell culture case convenient to observe |
CN114532300A (en) * | 2022-03-14 | 2022-05-27 | 徐州医科大学 | Detectable nematode motion and action and incubator of piling up culture dish |
CN216998393U (en) * | 2022-03-23 | 2022-07-19 | 嘉兴维亚生物科技有限公司 | Microbial cultivation inspection combination box |
CN115078747A (en) * | 2022-07-01 | 2022-09-20 | 厦门元谱生物科技有限公司 | Automatic sample feeding and withdrawing device for culture dish |
JP7237391B1 (en) * | 2022-01-24 | 2023-03-13 | 大和酸素工業株式会社 | C. elegans automatic transfer device |
-
2023
- 2023-07-28 CN CN202310935153.4A patent/CN116656496B/en active Active
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013129558A1 (en) * | 2012-02-29 | 2013-09-06 | 株式会社ジェイテック | Cell culture device having culture medium replacement function |
CN105494219A (en) * | 2015-12-14 | 2016-04-20 | 中国水产科学研究院黄海水产研究所 | Aquaculture pond with vortex sewage discharge performance |
CN210458219U (en) * | 2019-07-19 | 2020-05-05 | 无锡菩禾生物医药技术有限公司 | Multi-layer cell culture box easy to take and place bottom culture dish |
CN211631428U (en) * | 2019-12-30 | 2020-10-09 | 广西华崧生物防控科技有限公司 | Box-type culture dish for culturing entomopathogenic nematodes |
CN111454836A (en) * | 2020-04-14 | 2020-07-28 | 青岛农业大学 | Marine bacteria culture device |
CN113564047A (en) * | 2021-08-09 | 2021-10-29 | 董玉娟 | Culture dish for biological stem cell culture and matched incubator thereof |
JP7237391B1 (en) * | 2022-01-24 | 2023-03-13 | 大和酸素工業株式会社 | C. elegans automatic transfer device |
CN114456913A (en) * | 2022-02-22 | 2022-05-10 | 宣卫东 | Cell culture case convenient to observe |
CN114532300A (en) * | 2022-03-14 | 2022-05-27 | 徐州医科大学 | Detectable nematode motion and action and incubator of piling up culture dish |
CN216998393U (en) * | 2022-03-23 | 2022-07-19 | 嘉兴维亚生物科技有限公司 | Microbial cultivation inspection combination box |
CN115078747A (en) * | 2022-07-01 | 2022-09-20 | 厦门元谱生物科技有限公司 | Automatic sample feeding and withdrawing device for culture dish |
Non-Patent Citations (1)
Title |
---|
一种新型医学检验微生物培养箱的研制与应用;钟平 等;;中国医学装备(2);130-133 * |
Also Published As
Publication number | Publication date |
---|---|
CN116656496A (en) | 2023-08-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN116656496B (en) | Marine phage culture device | |
CN107972999B (en) | Freezing box storage tank | |
CN109673626B (en) | Combined type low-temperature access device for biological samples | |
CN117586871B (en) | Temperature control equipment for cell culture | |
CN111217070B (en) | Can automatic access sample's breeding device | |
CN212426064U (en) | Electric heating constant temperature incubator | |
CN114162446B (en) | Portable rapid detection kit for food inspection | |
CN114574545B (en) | Small target monitoring method based on multi-scale super-resolution | |
CN114532300B (en) | Detectable nematode motion and action and incubator of piling up culture dish | |
WO2022193406A1 (en) | Accurate sample separation apparatus for water body sediment sample | |
CN213295346U (en) | Sample fungus taking and scribing rotating mechanism | |
CN214418819U (en) | Intelligent sample cabinet for laboratory | |
CN111778150B (en) | Drawing type biological incubator | |
CN114410461A (en) | Novel integrative formula microorganism inspection device | |
CN113897289A (en) | Cell storage and culture device | |
CN220432794U (en) | Microorganism culture device convenient to store | |
CN221254558U (en) | Biochemical incubator | |
CN113265329B (en) | Biological cell incubator and use method thereof | |
CN221566124U (en) | Microorganism incubator | |
CN219117428U (en) | Rack for microbial cell culture | |
CN215050373U (en) | Cell culture device of adaptable not unidimensional culture dish | |
CN209871079U (en) | Placing kit for yang blood culture bottle and seed transfer culture dish | |
CN220019093U (en) | High-throughput automatic chromosome pelleter | |
CN221501075U (en) | Microorganism culture apparatus convenient to sample and detect | |
CN221260584U (en) | Diversified selective liquid sucking and injecting device |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20230829 Assignee: QINGDAO INSIGHT EXBIO Co.,Ltd. Assignor: YELLOW SEA FISHERIES Research Institute CHINESE ACADEMY OF FISHERY SCIENCES Contract record no.: X2024370010002 Denomination of invention: A marine bacteriophage culture device Granted publication date: 20231027 License type: Common License Record date: 20240104 |