CN117343825B - Marine microorganism strain storage device - Google Patents

Marine microorganism strain storage device Download PDF

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Publication number
CN117343825B
CN117343825B CN202311648468.7A CN202311648468A CN117343825B CN 117343825 B CN117343825 B CN 117343825B CN 202311648468 A CN202311648468 A CN 202311648468A CN 117343825 B CN117343825 B CN 117343825B
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frame
driving
storage
sleeve
layer separation
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CN117343825A (en
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樊洪岩
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Binzhou Xinrong Bio Fertilizer Co ltd
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Binzhou Xinrong Bio Fertilizer Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/22—Means for packing or storing viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of storage devices, and provides a marine microorganism strain storage device which can realize centralized storage of a plurality of marine microorganism strains, has independent storage spaces and good practicality, is convenient for storage and extraction of the marine microorganism strains, and has good storage convenience.

Description

Marine microorganism strain storage device
Technical Field
The invention relates to the technical field of storage devices, in particular to a marine microorganism strain storage device.
Background
It is known that moisture and temperature are one of the basic conditions for microbial bacterial growth, so that a low-temperature and low-water environment is mostly adopted for microbial bacterial storage, the basic conditions for maintaining microbial bacterial survival are formed, and meanwhile activities of microbial bacteria are reduced, and the marine microbial strain storage device is a storage device for long-term maintenance of microbial strains in the ocean.
Through searching, the invention patent with the Chinese patent application number of CN202223412909.7 discloses a high-safety strain storage device, which is roughly described as comprising a body, a storage box and a heat preservation box, wherein the storage box is arranged in the body, the surface of the storage box is in clamping connection with the heat preservation box, the top end of the heat preservation box is in clamping connection with a top cover, the bottom surface of the storage box is provided with a circular clamping groove, the inside of the storage box is fixedly connected with a sealing cavity, the bottom end of the sealing cavity is provided with a sealing ring, a moving rod is connected in the storage box in a penetrating way, the moving rod is in clamping connection with the circular clamping groove or the sealing ring, the top surface of the moving rod is fixedly connected with a sealing plate, when the strain storage device is used, the thickness of the storage box is increased through a plastic layer, a second heat insulation layer and a rock wool layer, so that the storage box is not easy to damage, the safety of the storage device is enhanced, the outside of the storage device can be effectively protected, in addition, the heat preservation box also has a heat preservation function, then the top cover is taken down on the heat preservation box, and the first annular protruding block of the moving rod is clamped with the circular clamping groove, at the moment, the sealing plate is ejected from the storage box, so that a small amount of strains can be conveniently poured out, waste is avoided, then the second annular protruding block of the moving rod is clamped with the sealing ring, the sealing ring and the second annular protruding block seal the bottom end of the storage box, so that strains can be prevented from pouring out, the principle of a freeze drying method is that microbial cells are dehydrated in a sublimation manner under the freezing and decompression conditions according to the technical regulations of microbial strain resource freeze drying and low-temperature freeze preservation, physiological activities of the cells tend to stop, the survival state can be maintained for a long time, an ampoule pipe or a spherical ampoule pipe with uniform pipe wall thickness and neutral glass is generally adopted for freeze drying, the split charging amount of each pipe is about O.l-O.2 ml if the ampoule pipe is the spherical ampoule pipe, the inner diameter of the half ball part pipe is about 8mm, and the length is not less than lOOmm.
The prior art proposal provides a storage device for strains, but the marine microorganism strain system is huge, so that the method can ensure long-term good storage of marine microorganisms, realize centralized storage of a large number of marine microorganism strains, and become one of the keys for reducing the cost of storing the marine microorganism strains and improving the storage convenience.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides the marine microorganism strain storage device which can realize concentrated storage of various marine microorganism strains, has independent storage spaces, is practical and convenient for the storage and extraction of the marine microorganism strains, and has good storage convenience.
(II) technical scheme
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a marine microorganism bacterial storage device, includes a plurality of case, including main part jar and a plurality of neutral glassware, main part jar internal rotation is connected with a plurality of layers of separation frame, and is a plurality of one layer separation frame of downside is connected with the interior bottom wall rotation of main part jar in the layer separation frame, all rotates between every two layers separation frames adjacent about in the plurality of layers separation frame to be connected, all is provided with a plurality of storage chambers in the plurality of layers separation frame, and a plurality of all offer the location internal orifice in the storage chamber, a plurality of the storage chamber respectively with a plurality of the case matches, all installs the centre gripping clamp in the plurality of case, a plurality of the centre gripping clamp matches with a plurality of neutral glassware respectively, and a plurality of equal sliding connection has the insertion sleeve in the insertion sleeve, and a plurality of all be connected with the soft spring in the insertion sleeve, a plurality of soft spring respectively with a plurality of the actuating lever is connected with, and a plurality of insertion sleeve all sliding connection have the limiting plate, all are provided with on a plurality of actuating levers and all rotate the limiting plate and have set up in the drive sleeve, and draw out the structure in a plurality of the side and draw out the main part jar and the structure and draw out in the main part jar, install the structure and draw out in the side.
Preferably, the picking and placing structure comprises two synchronous driving rollers and two synchronous driven rollers, the two synchronous driving rollers and the two synchronous driven rollers are all rotationally connected in the side protruding chamber, two servo motors are installed outside the side protruding chamber, the two servo motors are respectively used for rotation driving and control of the two synchronous driving rollers, the two synchronous driving rollers are all in transmission connection with driving belts, the two driving belts are respectively in transmission connection with the two synchronous driven rollers, the two driving belts are respectively in rotation connection with a first connecting frame and a second connecting frame, an extraction frame is connected between the first connecting frame and the second connecting frame, a magnetic attraction positioning system is arranged between the extraction frame and the storage box, and a posture stabilizing component is installed between the two driving belts and used for maintaining the posture of the extraction frame.
Further, the magnetic attraction positioning system comprises a conductor and a plurality of iron blocks, wherein the iron blocks are fixedly connected with traction rods, the traction rods are respectively and slidably connected in the storage boxes, the traction rods are respectively connected with a plurality of driving rods, the conductor is installed in the extraction frame, a wire is wound on the conductor, the conductor and the wire can be matched to form a magnetic field for magnetic attraction of the iron blocks through controlling current formed in the wire, and a magnetic attraction hook structure matched with the iron blocks is installed in the extraction frame.
Preferably, the magnetic hooking structure comprises a sliding hook head, the sliding hook head is slidably connected in the extracting frame, a tension spring connected with the sliding hook head is fixedly connected in the extracting frame, a hooking plane matched with the sliding hook head is arranged on the iron block, the sliding hook head is positioned below the conductor and the conductor, and the sliding hook head moves upwards relative to the extracting frame under the action of a magnetic field generated by the conductor and the conductor, so that the sliding hook head and the hooking plane form a relative action, and the hooking connection of the extracting frame to the storage box is realized.
Further, the gesture stabilizing assembly comprises a middle frame, two sliding plates are connected to the middle frame in a sliding manner, two centering springs are connected to the two sliding plates, the two centering springs are connected to the middle frame, the two sliding plates are respectively connected with a first connecting frame in a rotating manner and a second connecting frame in a rotating manner, a sliding sleeve is fixedly connected to the middle frame, a guide rod is connected to the sliding sleeve in a sliding manner, and the guide rod is fixedly connected with the extraction frame.
Preferably, the driving structure comprises a middle rotating column, a lifting sleeve is connected to the middle rotating column, a driving motor for driving the middle rotating column to rotate is arranged at the bottom end of the main body tank, an adjusting motor is arranged at the top end of the middle rotating column, a lead screw is arranged on an output shaft of the adjusting motor, a middle groove cavity for placing the lead screw is formed in the middle rotating column, a thread sleeve is connected to the lead screw in a threaded manner, and the thread sleeve is connected with the lifting sleeve and used for driving the multiple layers of separating frames to rotate.
Further, the threaded sleeve is connected with a fin plate through a cylinder, the fin plate is connected with the lifting sleeve, and the middle rotating column is provided with a sliding through groove matched with the fin plate.
Preferably, a plurality of driving teeth are arranged on the outer side face of the lifting sleeve, a plurality of driven teeth matched with the driving teeth are arranged in the layer separation frame, and the number of the driven teeth in the layer separation frame is an integral multiple of the number of the storage cavities in the layer separation frame.
Further, an auxiliary bracket is arranged at the bottom end of the main body tank.
Still further, be provided with a plurality of income gas quick-connect pipes and a plurality of exhaust quick-connect pipes on the main part jar.
(III) beneficial effects
Compared with the prior art, the invention provides a marine microorganism strain storage device, which has the following beneficial effects:
1. according to the invention, through the matching of the main body tank and the layer separation frame, a plurality of storage cavities with changeable positions are formed, so that the storage boxes can be conveniently placed and stored, and the concentrated storage of various marine microorganism strains can be realized.
2. According to the invention, the direct storage units of a plurality of marine microorganism strains are realized through the cooperation of the storage box, the neutral glassware and the clamping clamp, and each storage space is independent.
3. According to the invention, through the design of the taking and placing structure, the plurality of storage boxes are convenient to put in and take out relative to the plurality of storage cavities, so that the marine microorganism strain can be conveniently put in and taken out for storage, and the storage convenience is good.
4. According to the invention, through the design of the driving structure, a structure for rotating, adjusting and driving the plurality of layers of separation frames in the main tank is formed, so that the storage boxes in the plurality of storage cavities can be conveniently adjusted relative to the taking and placing structure, and the practicability is good.
Drawings
FIG. 1 is a schematic view of a partially sectioned perspective structure of the present invention;
FIG. 2 is a schematic view of a partial enlarged structure of the present invention at A in FIG. 1;
FIG. 3 is a schematic view of a partially enlarged structure of the present invention at B in FIG. 1;
FIG. 4 is a schematic perspective view of the cooperation of the storage box, the neutral glass ware, the clamping clips and the like;
FIG. 5 is a schematic perspective view, partially in section, of the cooperating storage case, neutral glass ware, clamping clip, etc. of the present invention;
FIG. 6 is a schematic perspective view of a layer separator of the present invention;
FIG. 7 is a schematic view of a partially cut-away perspective structure of the first connector, second connector, and extractor of the present invention;
FIG. 8 is a schematic view of a partially sectioned perspective view of another embodiment of the present invention in which a first connector, a second connector, and a pick-up rack cooperate;
FIG. 9 is a schematic top perspective view of the entire present invention;
FIG. 10 is a schematic view of the overall bottom perspective of the present invention;
FIG. 11 is a schematic view of a partial bottom view in section of the present invention;
FIG. 12 is a schematic view of a partial cross-sectional perspective structure of the invention with the rotor post, lifting sleeve, lead screw, etc. engaged;
FIG. 13 is a schematic exploded perspective view of the lifting sleeve, threaded sleeve, fin plate, etc. of the present invention;
fig. 14 is a schematic view showing a partially cut-away perspective structure of the cooperation of the insertion sheath, the driving rod, the soft spring, etc. of the present invention.
In the figure: 1. a storage box; 2. a main body tank; 3. a neutral glass vessel; 4. a layer separation frame; 5. a storage chamber; 6. positioning an inner opening; 7. a clamping clip; 8. an insertion sleeve; 9. a driving rod; 10. a soft spring; 11. a limiting plate; 12. driving the groove; 13. a hard spring; 14. a side protruding chamber; 15. synchronous driving rollers; 16. synchronizing the driven rollers; 17. a transmission belt; 18. a first connection frame; 19. a second connecting frame; 20. an extraction frame; 21. a conductor; 22. iron blocks; 23. a traction rod; 24. a wire; 25. sliding hook heads; 26. a tension spring; 27. a hooking plane; 28. a middle frame; 29. a sliding plate; 30. a centering spring; 31. a sliding sleeve; 32. a guide rod; 33. a middle rotating column; 34. a lifting sleeve; 35. a screw rod; 36. a thread sleeve; 37. a fin plate; 38. sliding through grooves; 39. a drive tooth; 40. driven teeth; 41. an auxiliary bracket; 42. a gas inlet quick connecting pipe; 43. and (5) an exhaust quick connection pipe.
Detailed Description
Examples: referring to fig. 1-14, a marine microorganism strain storage device comprises a plurality of storage boxes 1, including a main body tank 2 and a plurality of neutral glassware 3, an auxiliary support 41 is installed at the bottom end of the main body tank 2 to form an auxiliary support for the main body tank 2, a plurality of gas inlet quick connecting pipes 42 and a plurality of gas outlet quick connecting pipes 43 are arranged on the main body tank 2 to facilitate the formation of a low-temperature oxygen-isolation space in the main body tank 2, a plurality of layer separation frames 4 are rotationally connected in the main body tank 2, one layer separation frame 4 at the lowest side of the plurality of layer separation frames 4 is rotationally connected with the inner bottom wall of the main body tank 2, every two layer separation frames 4 adjacent to each other up and down in the plurality of layer separation frames 4 are rotationally connected, a plurality of storage cavities 5 are arranged in the plurality of layer separation frames 4, through the cooperation of the main body tank 2 and the layer separation frames 4, a plurality of storage cavities 5 with changeable positions are formed to facilitate the placement and storage of the plurality of storage boxes 1, the concentrated storage of a plurality of marine microorganism strains can be realized, the storage cavities 5 are provided with positioning inner openings 6, the storage cavities 5 are respectively matched with the storage boxes 1, the storage boxes 1 are provided with clamping clamps 7, the clamping clamps 7 are respectively matched with the neutral glassware 3, the direct storage units of the marine microorganism strains are realized through the matching of the storage boxes 1, the neutral glassware 3 and the clamping clamps 7, the storage spaces are independent, the storage boxes 1 are respectively provided with an insertion sleeve 8 in a sliding manner, the insertion sleeves 8 are respectively provided with a driving rod 9 in a sliding manner, the insertion sleeves 8 are respectively provided with a soft spring 10, the soft springs 10 are respectively connected with the driving rods 9, the insertion sleeves 8 are respectively provided with a limiting plate 11 in a sliding manner, the driving rods 9 are respectively provided with driving grooves 12 which are respectively and rotationally driven by the limiting plates 11, the utility model provides a plurality of insert cover 8 all are connected with hard spring 13 outward, a plurality of hard springs 13 are fixed connection respectively in a plurality of case 1, install the drive structure in main part jar 2, drive structure is used for the rotation drive and the adjustment of a plurality of layers of separation frame 4, drive structure includes well swivel post 33, be connected with lifting sleeve 34 on the well swivel post 33, the driving motor who is used for well swivel post 33 rotation drive is installed to the bottom of main part jar 2, the adjustment motor is installed on the top of well swivel post 33, install lead screw 35 on the output shaft of adjustment motor, set up the well groove chamber that is used for the lead screw 35 to put into on the well swivel post 33, threaded connection has thread bush 36 on the lead screw 35, thread bush 36 is connected with lifting sleeve 34, the rotation drive of a plurality of layers separation frame 4 is used for, fixedly connected with the drum on the thread bush 36, fixedly connected with wing plate 37 on the drum, be connected with lifting sleeve 34, set up with a plurality of driving teeth 39 on the lateral surface of well swivel post 33, all be provided with the well groove 38 with the number of driven tooth frame 40 of a plurality of layers of tooth frame 4, the number of 3 is for the number of layers of drive structure is adjusted, the number of 3 is realized for the 3, the number of layers of 3 is realized for the 3, the number of layers of 3 is adjusted, and the number of layers of 3, and is stored in the number is convenient, and is stored in the number, and is 5, and is stored and is more than the number and is more than 5, and is more than the number and is 5.
It should be further noted that, the main body tank 2 is provided with a side protruding chamber 14, a picking and placing structure is installed in the side protruding chamber 14, the picking and placing structure is used for picking and placing a plurality of storage boxes 1 in the main body tank 2, the picking and placing structure comprises two synchronous driving rollers 15 and two synchronous driven rollers 16, the two synchronous driving rollers 15 and the two synchronous driven rollers 16 are both rotationally connected in the side protruding chamber 14, two servo motors are installed outside the side protruding chamber 14, the two servo motors are respectively used for rotationally driving and controlling the two synchronous driving rollers 15, the two synchronous driving rollers 15 are both in transmission connection with a driving belt 17, the two driving belts 17 are respectively in transmission connection with the two synchronous driven rollers 16, the two driving belts 17 are respectively in rotational connection with a first connecting frame 18 and a second connecting frame 19, an extracting frame 20 is connected between the first connecting frame 18 and the second connecting frame 19, a magnetic attraction positioning system is arranged between the extraction frame 20 and the storage box 1, the magnetic attraction positioning system comprises a conductor 21 and a plurality of iron blocks 22, the iron blocks 22 are fixedly connected with a traction rod 23, the traction rods 23 are respectively and slidably connected in the storage box 1, the traction rods 23 are respectively connected with a plurality of driving rods 9, the conductor 21 is arranged in the extraction frame 20, a wire 24 is wound on the conductor 21, the conductor 21 and the wire 24 can be matched to form a magnetic field for magnetically attracting the iron blocks 22 by controlling the current formed in the wire 24, a magnetic attraction hook structure matched with the iron blocks 22 is arranged in the extraction frame 20, the magnetic attraction hook structure comprises a sliding hook head 25, the sliding hook head 25 is slidably connected in the extraction frame 20, a tension spring 26 connected with the sliding hook head 25 is fixedly connected in the extraction frame 20, a hook plane 27 matched with the sliding hook head 25 is arranged on the iron blocks 22, the sliding hook head 25 is positioned below the conductor 21 and the wire 24, under the effect of the magnetic field generated by the conductor 21 and the wire 24, the sliding hook head 25 moves upwards relative to the extracting rack 20, thereby realizing the relative effect between the sliding hook head 25 and the hook plane 27, realizing the hook connection of the extracting rack 20 to the storage boxes 1, facilitating the putting in and taking out of the storage boxes 1 relative to the storage cavities 5 respectively through the design of the taking-out structure, thereby facilitating the putting in and taking out of marine microorganism strain storage, the storage convenience is better, an attitude stabilizing assembly is arranged between the two driving belts 17, the attitude stabilizing assembly comprises a middle rack 28, two sliding plates 29 are connected on the middle rack 28 in a sliding way, two sliding plates 29 are connected with centering springs 30, the two centering springs 30 are connected in the middle rack 28, the two sliding plates 29 are respectively connected with the first connecting rack 18 in a rotating way and the second connecting rack 19 in a rotating way, a sliding sleeve 31 is fixedly connected on the middle rack 28, a guide rod 32 is connected with the extracting rack 20 in a sliding way, and the attitude stabilizing assembly is used for maintaining the attitude of the extracting rack 20.
The servo motor, the driving motor and the adjusting motor in this embodiment are all conventional devices known to those skilled in the art and purchased in the market, in this patent, only the servo motor, the driving motor and the adjusting motor are used, the structure and the function of the servo motor are not improved, the setting mode, the installation mode and the electric connection mode of the servo motor, the driving motor and the adjusting motor are only required to be debugged according to the requirements of the application specification of the servo motor, and the servo motor, the driving motor and the adjusting motor are not described again herein.
In summary, the marine microorganism strain storage device is used in the process that, when in use, firstly, the marine microorganism strain storage device is erected and installed at a place required to be used, the air inlet quick connection pipe 42 and the air outlet quick connection pipe 43 are communicated with an external circulation air path system, the medium for forming cooling and oxygen isolation can be nitrogen or other similar mediums, the lead 24, the servo motor, the driving motor and the adjusting motor are respectively connected with a forward and reverse rotation circuit according to respective specifications, meanwhile, an intelligent control panel can be additionally arranged at a proper position of the main body tank 2 or the side protruding chamber 14 if necessary, so as to form the collocation control of the lead 24, the servo motor, the driving motor and the adjusting motor, the two servo motors work to respectively realize the motion driving of the two driving belts 17, when the two servo motors control the two driving belts 17 to synchronously move in the same direction, the height of the extraction frame 20 is changed along with the synchronous and same-direction movement of the two driving belts 17, when the two servo motors control the two driving belts 17 to synchronously and reversely move so as to separate the two sliding plates 29 from each other, the two driving belts 17 control the relative included angle between the first connecting frame 18 and the second connecting frame 19 to be increased, so that the extraction frame 20 is close to the driving belts 17, when the two servo motors control the two driving belts 17 to synchronously and reversely move so as to separate the two sliding plates 29 from each other, the two driving belts 17 control the relative included angle between the first connecting frame 18 and the second connecting frame 19 to be reduced, so that the extraction frame 20 is separated from the driving belts 17, the driving motor works to realize the rotation driving of the middle rotating column 33, the rotation of the middle rotating column 33 drives the lifting sleeve 34 to form synchronous rotation, the adjustment motor works to realize the rotation driving of the lead screw 35, under the cooperation of the screw rod 35 and the threaded sleeve 36, the rotating screw rod 35 forms the change of the height of the threaded sleeve 36 relative to the middle rotary column 33, the change of the height of the threaded sleeve 36 can realize the change of the height of the lifting sleeve 34 relative to the multi-layer separation frames 4, so that the effective adjustment of the driving teeth 39 relative to the driven teeth 40 with different heights is convenient, and certain friction exists between the multi-layer separation frames 4 and the main tank 2, so that friction resistance is formed, and under the action of the friction resistance, the rotating lifting sleeve 34 only forms driving for the layer separation frames 4 corresponding to the driven teeth 40 with the effective action of the driving teeth 39 on the lifting sleeve 34.
Further, when marine microorganism strains are required to be stored, marine microorganism strains are firstly pretreated, the pretreated marine microorganism strains are placed into the corresponding neutral glassware 3 and form a seal, meanwhile, the neutral glassware 3 is subjected to necessary identification, meanwhile, the identification information is input into an intelligent control panel to form information storage, then the neutral glassware 3 is placed into the corresponding storage box 1 through the clamping clamp 7, then two servo motors are formed through the intelligent control panel to work, the two driving belts 17 synchronously move in the same direction to lift the extraction rack 20 from the side protruding chamber 14, after the extraction rack 20 can be exposed from the taking and placing opening on the side protruding chamber 14, the storage box 1 placed into the neutral glassware 3 is placed in an alignment position relative to the exposed extraction rack 20, and a lead 24 is controlled to be connected with current through the intelligent control panel, the wire 24 and the conductor 21 are matched to form a magnetic field, the magnetic field can overcome the elastic force of the soft spring 10, the hard spring 13 and the tension spring 26, the driving rod 9 can move relative to the insertion sleeve 8 to form a relative position, the insertion sleeve 8 can move relative to the storage box 1 to form a position, the sliding hook head 25 can move relative to the storage box 1 to form a position, when the driving rod 9 moves relative to the insertion sleeve 8 to form a relative position, the pushing action of the limiting plate 11 fails, the limiting plate 11 reduces relative height relative to the insertion sleeve 8, when the insertion sleeve 8 moves relative to the storage box 1 to form a position, the traction rod 23 moves relative to the storage box 1 to form a position, thereby facilitating the removal of the iron block 22 from the storage box 1 into the extraction frame 20, and the limiting of the iron block 22 is formed due to the action of lifting the sliding hook head 25, so that the limiting of the relative position of the storage box 1 relative to the extraction frame 20 is achieved, then, the operator can relax the holding force for keeping the position of the storage box 1, then the two servo motors work to realize the synchronous and same-direction movement of the two driving belts 17, realize the height change of the extraction frame 20 relative to the side protruding chamber 14, achieve the height adjustment of the storage box 1, and after the height adjustment of the storage box 1 to the proper height of the storage cavity 5, the two servo motors change the working mode to control the two driving belts 17 to enable the two sliding plates 29 to approach each other so as to form the reduction of the relative included angle of the first connecting frame 18 and the second connecting frame 19, so that the extraction frame 20 is far away from the driving belts 17, thereby facilitating the pushing of the storage box 1 into the corresponding storage cavity 5.
Continuing further, when the storage box 1 is completely inserted into the corresponding storage cavity 5, the exposed insertion sleeve 8 corresponding to the storage box 1 is inserted into the corresponding positioning inner opening 6, then the current in the lead 24 is cut off, so that the magnetic field formed by the matching of the lead 24 and the conductor 21 is disabled, the sliding hook head 25 is lowered and reset relative to the forming position of the storage box 1 under the elastic reset trend of the soft spring 10, the hard spring 13 and the tension spring 26, the limiting action of the sliding hook head 25 on the iron block 22 is disabled under the state, the insertion sleeve 8 is further inserted into the corresponding positioning inner opening 6, and the driving rod 9 forms a position change relative to the insertion sleeve 8 to push the limiting plate 11 upwards relative to the insertion sleeve 8 to form the limit in the positioning inner opening 6 along with the insertion of the insertion sleeve 8 when the limiting plate 11 is inserted into the position of the insertion sleeve 8, when a certain marine microorganism strain is required to be taken out, the storage position of the marine microorganism strain which is required to be taken out is rotated into a position corresponding to the side protruding chamber 14 by driving the structure to work, then the two servo motors work, the approaching movement of the extracting rack 20 relative to the corresponding storage box 1 is realized until the storage box 1 is abutted, the current communication is formed for the lead 24, under the action of a magnetic field generated by matching the lead 24 with the conductor 21, the elasticity of the lead 10 is weaker than that of the hard spring 13 due to the comparison of the soft spring 10 and the hard spring 13, so that the driving rod 9 moves relative to the insertion forming position, the hard spring 13 is stressed to be compressed after the gravity of the limiting plate 11 relative to the insertion sleeve 8 is reduced, the position of the insertion sleeve 8 relative to the storage box 1 is realized, after the sliding hook head 25 is lifted to form the hooking limit for the iron block 22, the position change of the extracting frame 20 can be realized by controlling the matching work of two servo motors, the corresponding storage box 1 is taken out of the storage cavity 5, and meanwhile, in order to ensure the synchronization of the environment in the main body tank 2 and the environment in the storage cavity 5, the layer separation frame 4 is provided with a ventilation gap, in order to ensure the consistency of the storage box 1 placed in the storage cavity 5 and the environment in the storage cavity 5 and to avoid the effective separation between the space in the storage box 1 and the side protruding chamber 14 at the adjacent position in the side protruding chamber 14, the top of the storage box 1 adopts a structure gradually lifted from inside to outside, so that the effective communication of the storage box 1 and the storage cavity 5 is also convenient when the effective sealing of the outer ring of the storage box 1 and the main body tank 2 and the layer separation frame 4 is ensured.

Claims (6)

1. The utility model provides a marine microorganism bacterial storage device, includes a plurality of case (1), its characterized in that still includes main part jar (2) and a plurality of neutral glassware (3), main part jar (2) internal rotation is connected with a plurality of layer separation frame (4), a plurality of in layer separation frame (4) one layer separation frame (4) and main part jar (2) interior bottom wall rotation are connected, and every two layer separation frame (4) that are adjacent from top to bottom in a plurality of layer separation frame (4) are all rotated between being connected, all are provided with a plurality of storage chamber (5) in a plurality of layer separation frame (4), a plurality of all offer in a plurality of storage chamber (5) location internal orifice (6), a plurality of storage chamber (5) respectively with a plurality of case (1) are matchd, a plurality of holding clamp (7) are all installed in a plurality of case (1) respectively with a plurality of neutral glassware (3) are matchd, all sliding connection has plug-in sleeve (8) in a plurality of case (1), all sliding connection has plug-in sleeve (8), all sliding connection (8) have a plurality of plug-in rod (8) and a plurality of plug-in sleeve (10) are connected with a plurality of soft spring (10 respectively, the driving grooves (12) which are all rotationally driven by the limiting plates (11) are arranged on the driving rods (9), hard springs (13) are connected outside the inserting sleeves (8), the hard springs (13) are fixedly connected in the storage boxes (1) respectively, a driving structure is arranged in the main body tank (2), the driving structure is used for rotationally driving and adjusting the separating frames (4) of a plurality of layers, a side protruding chamber (14) is arranged on the main body tank (2), a picking and placing structure is arranged in the side protruding chamber (14), the picking and placing structure is used for lifting and placing the storage boxes (1) in the main body tank (2), the picking and placing structure comprises two synchronous driving rollers (15) and two synchronous driven rollers (16), the synchronous driving rollers (15) and the two synchronous driven rollers (16) are rotationally connected in the side protruding chamber (14), two servo motors are arranged outside the side protruding chamber (14), the two servo motors are respectively used for controlling the two synchronous driving rollers (15) to rotate respectively, the two driving rollers (17) are connected with a first transmission belt (17) and a second transmission belt (17) respectively, the utility model is characterized in that an extraction frame (20) is connected between the first connecting frame (18) and the second connecting frame (19), a magnetic attraction positioning system is arranged between the extraction frame (20) and the storage box (1), the magnetic attraction positioning system comprises a conductor (21) and a plurality of iron blocks (22), the iron blocks (22) are fixedly connected with traction rods (23), the traction rods (23) are respectively and slidingly connected in the storage boxes (1), the traction rods (23) are respectively connected with a plurality of driving rods (9), the conductor (21) is arranged in the extraction frame (20), and the conductor (21) is wound with a wire (24), the conductor (21) and the wire (24) can be matched to form a magnetic field for magnetically attracting the iron block (22) by controlling the current formed in the wire (24), a magnetic attraction hook structure matched with the iron block (22) is arranged in the extraction frame (20), the magnetic attraction hook structure comprises a sliding hook head (25), the sliding hook head (25) is slidingly connected in the extraction frame (20), a tension spring (26) connected with the sliding hook head (25) is fixedly connected in the extraction frame (20), a hook plane (27) matched with the sliding hook head (25) is arranged on the iron block (22), and slip gib head (25) are located below conductor (21) and wire (24), under the effect of the magnetic field that conductor (21) and wire (24) produced, slip gib head (25) form relative position and move up for drawing frame (20) to form relative action between slip gib head (25) and hook plane (27), realize drawing frame (20) to the hook connection of case (1), install gesture stabilizing assembly between two conveyer belts (17), gesture stabilizing assembly is used for drawing the gesture of frame (20) and keeps, gesture stabilizing assembly includes well frame (28), sliding connection has two slide plates (29) on well frame (28), two slide plates (29) all are connected with centering spring (30), two centering spring (30) all are connected in well frame (28), two slide plates (29) are connected with first link (18) and second link (19) rotation respectively, fixedly connected with sliding sleeve (31) on well frame (28), sliding sleeve (31) are connected with guide bar (32) on the guide bar (32).
2. The marine microorganism strain storage device according to claim 1, wherein the driving structure comprises a middle rotating column (33), a lifting sleeve (34) is connected to the middle rotating column (33), a driving motor for driving the middle rotating column (33) to rotate is arranged at the bottom end of the main tank (2), an adjusting motor is arranged at the top end of the middle rotating column (33), a lead screw (35) is arranged on an output shaft of the adjusting motor, a middle groove cavity for placing the lead screw (35) is formed in the middle rotating column (33), a threaded sleeve (36) is connected to the lead screw (35) in a threaded manner, and the threaded sleeve (36) is connected with the lifting sleeve (34), and the lifting sleeve (34) is used for driving the rotation of the multi-layer separation frame (4).
3. Marine microorganism strain storage device according to claim 2, characterized in that a fin plate (37) is connected to the threaded sleeve (36) through a cylinder, the fin plate (37) is connected to the lifting sleeve (34), and a sliding through groove (38) matched with the fin plate (37) is formed in the middle rotating column (33).
4. A marine microorganism strain storage device according to claim 3, wherein a plurality of driving teeth (39) are provided on the outer side surface of the lifting sleeve (34), a plurality of driven teeth (40) matched with the driving teeth (39) are provided in each of the layer separation frames (4), and the number of the driven teeth (40) in the layer separation frames (4) is an integer multiple of the number of the storage cavities (5) in the layer separation frames (4).
5. A marine microorganism strain storage device according to claim 4, wherein the bottom end of the main tank (2) is provided with an auxiliary support (41).
6. The marine microorganism strain storage device according to claim 5, wherein the main tank (2) is provided with a plurality of intake air fast connecting pipes (42) and a plurality of exhaust air fast connecting pipes (43).
CN202311648468.7A 2023-12-05 2023-12-05 Marine microorganism strain storage device Active CN117343825B (en)

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