CN113564047A - Culture dish for biological stem cell culture and matched incubator thereof - Google Patents

Culture dish for biological stem cell culture and matched incubator thereof Download PDF

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Publication number
CN113564047A
CN113564047A CN202110905263.7A CN202110905263A CN113564047A CN 113564047 A CN113564047 A CN 113564047A CN 202110905263 A CN202110905263 A CN 202110905263A CN 113564047 A CN113564047 A CN 113564047A
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axis
incubator
culture dish
matched
culture
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董玉娟
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/46Means for fastening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Clinical Laboratory Science (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a culture dish for culturing biological stem cells and a matched incubator thereof, wherein the culture dish comprises an incubator body, a chamber door is arranged on one side of the front surface of the incubator body, a control screen is embedded and installed above the other side of the front surface of the incubator body, a control button is fixedly installed below the other side of the front surface of the incubator body, and a bottom box is welded and installed at the bottom of the incubator body. This biological stem cell is culture dish and supporting incubator thereof has changed the artifical mode of placing of tradition, the operation of the artifical manual of mechanical automation drive, when placing or taking out the cultivation subassembly, the effectual open time of incubator body that has reduced, and then avoid the open time process of incubator body, the cultivation environmental change that leads to incubator body inside suffers destruction, reduce the destroyed possibility of the inside sample of incubator body, thereby improve the probability of incubator body cultivation success.

Description

Culture dish for biological stem cell culture and matched incubator thereof
Technical Field
The invention relates to the field of biological stem cell culture, in particular to a culture dish for biological stem cell culture and a matched incubator thereof.
Background
Living things refer to living things having kinetic energy, and are also a collection of objects. Whereas an individual organism refers to an organism, as opposed to a non-biological organism. In brief, a stem cell is a type of cell that has unlimited or immortal self-renewal capacity, and is capable of producing at least one type of highly differentiated progeny cell. The definition of stem cells has been revised and defined from different levels over the years. Most biologists and physicians now believe that stem cells are a class of cells derived from embryonic, fetal, or adult cells that have the ability to self-renew and proliferate and differentiate without restriction under certain conditions, and that are capable of producing daughter cells with the same phenotype and genotype as themselves, and also capable of producing specialized cells that make up body tissues and organs, and also capable of differentiating into progenitor cells.
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function.
When culturing the biological stem cells, a culture dish and an incubator are needed, and the culture dish is placed inside the incubator for culturing. However, the conventional incubator has a disadvantage that when the culture dish is placed or taken out, a cabinet door of the incubator needs to be opened, and then the culture dish is placed inside the incubator, but the incubator belongs to the centralized culture, and a plurality of culture dishes are placed together, so that when the culture dish is placed or taken out at a later stage, the opening time of the incubator is long, the internal environment of the incubator is broken at this time, and the probability of culture failure of the incubator is increased.
Disclosure of Invention
The invention aims to provide a culture dish for culturing biological stem cells and a matched incubator thereof, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a culture dish for biological stem cell culture and a matched culture box thereof comprise a culture box body, wherein a box door is arranged on one front side of the culture box body, a control screen is embedded and installed above the other front side of the culture box body, a control button is fixedly installed below the other front side of the culture box body, a bottom box is welded and installed at the bottom of the culture box body, six transverse moving structures and six culture dish placing frames are fixedly installed on one side of the culture box body at equal intervals, a culture assembly is arranged in a placing cavity of each culture dish placing frame, an inlet and an outlet are formed in the other side of the bottom of the culture box body in a penetrating mode, five culture dish through openings are formed in the other side of the top of the culture box body in a penetrating mode, the vertical section of each culture dish through opening is T-shaped, and five sealing structures are fixedly installed at the top of the culture box body and are positioned right above the culture dish, two Z-axis sliding rods are fixedly arranged between the bottom of the inner wall of the bottom box and the top of the inner wall of the incubator body, a Z-axis motor is fixedly arranged on one side of the bottom of the inner wall of the bottom box, a Z-axis lead screw is fixedly connected to the driving end of the Z-axis motor, a Z-axis moving structure is connected between the two Z-axis sliding rods and one Z-axis lead screw in a transmission manner and comprises a Z-axis base plate, five Z-axis moving blocks are slidably arranged on the top of the Z-axis base plate, a welding plate is fixedly connected between every two adjacent Z-axis moving blocks, a magnet strip is fixedly arranged on the side edge of one Z-axis moving block, convex strips are fixedly arranged on the top and the bottom of the magnet strip, two movable cavities are formed in one side of the Z-axis base plate, two electric telescopic rods are fixedly arranged on two sides of the top of the Z-axis moving block, and a Z-axis lifting plate is fixedly connected to the movable ends of the electric telescopic rods, the vacuum chuck is installed at the top of the Z-axis lifting plate in an inserting mode, a metal hose is fixedly connected with an air suction end of the vacuum chuck, a vacuum pump is fixedly installed in the middle of one side of the bottom of the Z-axis base plate, an adsorption pipeline is fixedly connected with the air suction end of the vacuum pump, the air suction end of the adsorption pipeline is fixedly connected with the air suction end of the metal hose, an electromagnetic valve is fixedly installed at the position of an interface of the adsorption pipeline and the adsorption pipeline, two electric telescopic rods I are fixedly installed at the bottom of the Z-axis base plate, a movable end of each electric telescopic rod I is fixedly connected with a magnetic block installation block, an electromagnet I is fixedly installed at the top of each magnetic block installation block, and an iron block is installed at the bottom of each Z-axis movable block in an embedding mode.
As a further scheme of the invention: the assembly is placed to culture dish rack includes that a curb plate and five household utensils place the subassembly, and the subassembly fixed connection is placed to the household utensils of curb plate and leftmost side, adjacent two the equal fixedly connected with middle part connecting block of opposite face that the subassembly was placed to the household utensils.
As a still further scheme of the invention: the household utensils are placed the subassembly and are included household utensils to place the board, the top and the top that the board was placed to the household utensils all run through and have been seted up five household utensils and place the hole, fixed mounting has five guide arms between the both sides that the inboard wall was placed to the household utensils, the household utensils are placed and are rotated through the bearing between the both sides of inboard portion and install five two-way threaded rods, and are adjacent two fly leafs, two are installed in the transmission between guide arm and the two-way threaded rod the equal fixed mounting of opposite face of fly leaf household utensils place the piece, the household utensils are placed the board and are rotated through the bearing of its bottom mosaic installation and install the vertical axis, be provided with the gear drive structure between the top of vertical axis and the two-way threaded rod.
As a still further scheme of the invention: one side of the movable plate is respectively provided with a threaded hole and a sliding hole in a penetrating mode, the movable plate is in sliding connection with the guide rod through the sliding hole, the sliding hole is in sliding connection with the guide rod, the movable plate is in transmission connection with the bidirectional threaded rod through the threaded hole, the bidirectional threaded rod is matched with the threaded hole, the moving directions of the two movable plates are in simultaneous belief or reverse movement, the top of the vessel placing block is provided with a placing groove, the gear transmission structure is composed of two bevel gears matched with each other, and the two bevel gears are meshed with each other.
As a still further scheme of the invention: the top of the Z-axis base plate is fixedly provided with five groups of Z-axis slide rails, the number of each group of Z-axis slide rails is two, the bottom of each Z-axis moving block is fixedly provided with two Z-axis moving block sliders matched with the Z-axis slide rails, the top of the Z-axis base plate is provided with two slide holes matched with the Z-axis slide rails, the Z-axis base plate is connected with the Z-axis slide rails in a sliding mode through the slide holes, the top of the Z-axis base plate is provided with threaded holes matched with Z-axis screw rods, and the Z-axis base plate is in transmission connection with the Z-axis screw rods through the threaded holes.
As a still further scheme of the invention: the culture assembly comprises a culture dish main body, a culture dish cover is arranged at the top of the culture dish main body, a convex rod is fixedly arranged at the top of the culture dish cover, and the top of the convex rod is in a circular truncated cone shape.
As a still further scheme of the invention: the lateral shifting structure includes horizontal frame, the passageway has been seted up in the front of horizontal frame the both sides of horizontal frame inner wall bottom are all rotated and are installed the installation axle, two the transmission is connected with belt drive structure between the installation axle, belt drive structure's conveyer belt outer wall fixed mounting has electro-magnet two, one side fixed mounting of horizontal frame inner wall bottom has driving motor two, be provided with belt pulley transmission structure between driving end and the left installation axle of driving motor two, the top of belt drive structure inner wall and the equal fixed mounting in the place ahead that the bottom just is located electro-magnet two have the sand grip spacing groove.
As a still further scheme of the invention: the belt wheel transmission structure is composed of two belt wheels and a belt, the two belt wheels are connected through belt transmission, the belt transmission structure is composed of two gear discs and a transmission belt, and the two gear discs are connected through the transmission belt.
As a still further scheme of the invention: the sealing structure comprises an inverted L-shaped frame, the inverted L-shaped frame is connected with a vertical rod matched with the inverted L-shaped frame through a sliding hole sliding mode formed in the top of the inverted L-shaped frame, a stop block is fixedly connected to the top of the vertical rod, a sealing block is fixedly connected to the bottom of the vertical rod, and a spring is sleeved between the sealing block and the inverted L-shaped frame on the outer wall of the vertical rod.
As a still further scheme of the invention: one side of the bottom of the Z-axis lifting plate is fixedly provided with a first driving motor, a cross block is fixedly arranged at the driving end of the first driving motor, a clamping seat is fixedly arranged at the bottom of the vertical shaft, and a cross groove matched with the cross block is formed in the bottom of the clamping seat.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, when a culture dish is placed, the Z-axis motor drives the Z-axis screw rod to rotate, the Z-axis base plate moves upwards under the vertical transmission action of the Z-axis screw rod and the Z-axis base plate, then the Z-axis lifting plate extends to the outside of the culture box body through the culture dish through hole, the vacuum chuck is exposed outside at the moment, the culture component is placed at the adsorption hole of the vacuum chuck, then the vacuum pump is started to generate negative pressure, the culture component is adsorbed and fixed through the adsorption pipeline, the metal hose and the vacuum chuck in sequence, the culture component is fixed in a vacuum adsorption type fixing mode, the culture component is convenient to absorb and place, and the five vacuum chucks can adsorb five culture components at the same time, so that the workload at the same time is greatly improved, and the efficiency is further improved.
2. After the culture components are adsorbed, the Z-axis base plate is made to descend into the interior of the incubator body, the magnet strips are aligned with the channels on the transverse frame at the current height, the magnet block mounting block is driven to move leftwards by the extension of the electric telescopic rod, the magnet strips enter the interior of the channels due to the magnetic adsorption of the electromagnet I and the iron block at the bottom of the Z-axis moving block, the convex strips enter the interior of the convex strip limiting grooves to slide, then the magnetism of the iron block at the bottom of the Z-axis moving block is relieved by the closing of the electromagnet I, meanwhile, the electromagnet II is started to carry out the magnetic adsorption with the magnet strips, the driving motor drives the left mounting shaft to rotate through the belt wheel transmission structure, and then the belt transmission structure is driven to rotate, so that the electromagnet II carries out the transverse movement, and the Z-axis moving block is driven to carry out the transverse suspension movement due to the magnetism of the electromagnet II and the magnet strips, can carry out vertical and horizontal all displaceable removal to the Z axle movable block, can carry out not co-altitude, position to cultivateing the subassembly and all can take away or place, the practicality is strong.
3. When the culture assembly is positioned right below the vessel placing hole, the electric telescopic rod II extends to drive the Z-axis lifting plate to move upwards, the cross block firstly enters the cross groove at the bottom of the clamping seat to be clamped, then the driving motor drives the cross block to rotate so as to drive the vertical shaft to rotate, the bidirectional threaded rod rotates along with the vertical shaft under the transmission action of the gear transmission structure, the two movable plates move towards two sides under the transverse transmission action of the two movable plates and the bidirectional threaded rod, the two movable plates contact with the vessel placing block to block the vessel placing hole at the bottom, then the electric telescopic rod II continuously extends out to enable the culture assembly to enter the interior of the vessel placing hole, the driving motor I rotates in the reverse direction, the two vessel placing blocks reset and limit and fix the culture assembly, the placement of the culture assembly is completed, otherwise, the culture assembly can be taken out and conveyed out of the interior of the culture box body from the culture vessel through hole, the mode that traditional manual work was placed has been changed, drives artifical manual operation by mechanical automation, when placing or taking out the cultivation subassembly, the effectual open time of incubator body that has shortened, and then avoids the open time course of incubator body, leads to the inside cultivation environmental change of incubator body to suffer destruction, reduces the destroyed possibility of the inside sample of incubator body to improve the probability that the incubator body cultivates successfully.
4. According to the invention, when the culture assembly is taken out, the convex rod on the culture assembly can be contacted with the bottom of the sealing block and can push the sealing block upwards, the sealing block can compress the spring at the moment, the stop block can be lifted upwards by hand to temporarily suspend the sealing block, the culture dish through opening is blocked by the sealing block on the sealing structure, the influence of the external environment on the environment inside the culture box body is further reduced, the environment protection effect on the inside of the culture box body is good, and the use effect of the culture box body is good.
Drawings
FIG. 1 is a schematic structural diagram of a culture dish for culturing biological stem cells and a matched incubator thereof;
FIG. 2 is a cross-sectional view of the main body of the culture dish and its complete incubator for culturing biological stem cells;
FIG. 3 is a front view of a Z-axis substrate in a culture dish for culturing biological stem cells and a matched incubator thereof;
FIG. 4 is a top view of a Z-axis substrate in a culture dish and its associated incubator for culturing biological stem cells;
FIG. 5 is a schematic structural diagram of a culture dish for culturing biological stem cells and a dish rack in a matched incubator;
FIG. 6 is a top view of a culture dish for culturing biological stem cells and a vessel placing plate in a matching incubator;
FIG. 7 is an enlarged schematic view of the portion A in FIG. 6 of a culture dish for culturing biological stem cells and a matched incubator thereof;
FIG. 8 is a schematic structural diagram of a culture dish for culturing biological stem cells and a culture assembly in a matched incubator thereof;
FIG. 9 is a front view of a culture dish for culturing biological stem cells and a transverse frame in an incubator matching with the culture dish;
FIG. 10 is a top cross-sectional view of a culture dish for culturing biological stem cells and a transverse frame in an incubator with the culture dish;
FIG. 11 is a schematic structural diagram of a sealing structure in a culture dish for culturing biological stem cells and a matched incubator thereof.
In the figure: the incubator comprises an incubator body 1, a chamber door 2, a bottom case 3, a control button 4, a control screen 5, a sealing structure 6, a transverse moving structure 7, a culture dish placing rack 8, a culture assembly 9, a culture dish through opening 10, a Z-axis screw rod 11, an inlet/outlet 12, a Z-axis moving structure 13, a Z-axis motor 14, a Z-axis slide rod 15, a vacuum pump 16, a first electric telescopic rod 17, a magnetic block mounting block 18, a Z-axis base plate 19, a Z-axis moving block slide block 20, a Z-axis slide rail 21, a Z-axis moving block 22, a second electric telescopic rod 23, a first driving motor 24, a Z-axis lifting plate 25, a cross block 26, a vacuum chuck 27, a metal hose 28, an adsorption pipeline 29, an electromagnetic valve 30, a convex strip 31, a magnet strip 32, a connecting and welding plate 33, a first electromagnet 34, a movable cavity 35, a side plate 36, a vessel placing assembly 37, a middle connecting block 38, a vertical shaft 39, a clamping seat 40, a vessel placing plate 41, a vessel placing hole 42, a horizontal moving structure 7, a culture dish through hole, a vacuum plate, a, The device comprises a bidirectional threaded rod 43, a gear transmission structure 44, a placing groove 45, a movable plate 46, a vessel placing block 47, a guide rod 48, a culture vessel main body 49, a culture vessel cover 50, a convex rod 51, a transverse frame 52, a channel 53, a driving motor II 54, a belt wheel transmission structure 55, a belt type transmission structure 56, a mounting shaft 57, a convex strip limiting groove 58, an electromagnet II 59, an inverted L-shaped frame 60, a sealing block 61, a vertical rod 62, a spring 63 and a stop block 64.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-11, the invention provides a technical scheme, a culture dish for biological stem cell culture and a matched culture box thereof, comprising a culture box body 1, a box door 2 is arranged on one side of the front surface of the culture box body 1, a control screen 5 is embedded and installed on the upper side of the other side of the front surface of the culture box body 1, a control button 4 is fixedly installed below the other side of the front surface of the culture box body 1, a bottom box 3 is welded and installed at the bottom of the culture box body 1, six transverse moving structures 7 and six culture dish placing frames 8 are fixedly installed on one side of the culture box body 1 at equal intervals, a culture assembly 9 is arranged in a placing cavity of each culture dish placing frame 8, an access 12 is arranged on the other side of the bottom of the culture box body 1 in a penetrating manner, five culture dishes 10 are arranged on the other side of the top of the culture box body 1 in a penetrating manner, and the vertical section of each culture dish through opening 10 is in a T shape, five sealing structures 6 are fixedly installed on the top of the incubator body 1 and located right above the culture dish through hole 10, two Z-axis slide bars 15 are fixedly installed between the bottom of the inner wall of the bottom box 3 and the top of the inner wall of the incubator body 1, a Z-axis motor 14 is fixedly installed on one side of the bottom of the inner wall of the bottom box 3, a Z-axis lead screw 11 is fixedly connected to the driving end of the Z-axis motor 14, a Z-axis moving structure 13 is connected between the two Z-axis slide bars 15 and one Z-axis lead screw 11 in a transmission manner, the Z-axis moving structure 13 comprises a Z-axis base plate 19, five Z-axis moving blocks 22 are slidably installed on the top of the Z-axis base plate 19, a connecting and welding plate 33 is fixedly connected between every two adjacent Z-axis moving blocks 22, a magnet strip 32 is fixedly installed on the side edge of one Z-axis moving block 22, protruding strips 31 are fixedly installed on the top and the bottom of the magnet strip 32, two movable cavities 35 are formed on one side of the Z-axis base plate 19, two sides of the top of the Z-axis moving block 22 are fixedly provided with electric telescopic rods II 23, the movable ends of the electric telescopic rods II 23 are fixedly connected with a Z-axis lifting plate 25, the top of the Z-axis lifting plate 25 is provided with a vacuum chuck 27 in an inserting way, the air suction end of the vacuum chuck 27 is fixedly connected with a metal hose 28, the middle part of one side of the bottom of the Z-axis substrate 19 is fixedly provided with a vacuum pump 16, the air suction end of the vacuum pump 16 is fixedly connected with an adsorption pipeline 29, and the air suction end of the adsorption pipeline 29 is fixedly connected with the air suction end of the metal hose 28, the electromagnetic valve 30 is fixedly installed at the joint of the adsorption pipeline 29 and the adsorption pipeline 29, the bottom of the Z-axis base plate 19 is fixedly provided with two electric telescopic rods I17, the movable end of each electric telescopic rod I17 is fixedly connected with a magnetic block installation block 18, the top of each magnetic block installation block 18 is fixedly provided with an electromagnet I34, and the bottom of the Z-axis movable block 22 is embedded with an iron block.
The culture dish placing frame 8 comprises a side plate 36 and five dish placing assemblies 37, the side plate 36 is fixedly connected with the dish placing assembly 37 on the leftmost side, and the opposite surfaces of the two adjacent dish placing assemblies 37 are fixedly connected with a middle connecting block 38.
The household utensils placing component 37 comprises a household utensils placing plate 41, five household utensils placing holes 42 are formed in the top and the top of the household utensils placing plate 41 in a penetrating mode, five guide rods 48 are fixedly mounted between two sides of the inner wall of the household utensils placing plate 41, five two-way threaded rods 43 are mounted between two sides of the household utensils placing plate 41 in a rotating mode through bearings, two movable plates 46 are mounted between the adjacent guide rods 48 and the two-way threaded rods 43 in a transmission mode, a household utensils placing block 47 is fixedly mounted on the opposite surfaces of the two movable plates 46, a vertical shaft 39 is mounted on the household utensils placing plate 41 in a rotating mode through the bearings mounted at the bottom of the household utensils placing plate 41 in an embedded mode, and a gear transmission structure 44 is arranged between the top of the vertical shaft 39 and the two-way threaded rods 43.
One side of the movable plate 46 is respectively provided with a threaded hole and a sliding hole in a penetrating manner, the movable plate 46 is in sliding connection with the guide rod 48 through the sliding hole, the sliding hole is in sliding connection with the guide rod 48, the movable plate 46 is in transmission connection with the bidirectional threaded rod 43 through the threaded hole, the bidirectional threaded rod 43 is matched with the threaded hole, the two movable plates 46 move in the same phase or in the opposite direction, the top of the vessel placing block 47 is provided with a placing groove 45, the gear transmission structure 44 is composed of two bevel gears matched with each other, and the two bevel gears are meshed with each other.
The top fixed mounting of Z axle base plate 19 has five groups Z axle slide rails 21, and the quantity of every group Z axle slide rail 21 has two, the bottom fixed mounting of Z axle movable block 22 has two Z axle movable block sliders 20 with Z axle slide rail 21 looks adaptation, and Z axle movable block slider 20 and Z axle slide rail 21 sliding connection, two slide holes with Z axle slide bar 15 looks adaptation are seted up at the top of Z axle base plate 19, and Z axle base plate 19 passes through slide hole and Z axle slide bar 15 sliding connection, the screw hole with Z axle lead screw 11 looks adaptation is seted up at the top of Z axle base plate 19, and Z axle base plate 19 passes through the screw hole and is connected with Z axle lead screw 11 transmission.
The culture assembly 9 comprises a culture dish main body 49, a culture dish cover 50 is arranged at the top of the culture dish main body 49, a convex rod 51 is fixedly arranged at the top of the culture dish cover 50, and the top of the convex rod 51 is in a circular truncated cone shape.
Lateral shifting structure 7 includes horizontal frame 52, and the both sides of horizontal frame 52 are open the form, the both sides of the horizontal frame 52 inner wall bottom of passageway 53 are seted up in the front of horizontal frame 52 all rotate and install installation axle 57, the transmission is connected with belt transmission structure 56 between two installation axles 57, belt transmission structure 56's conveyer belt outer wall fixed mounting has two electromagnets 59, one side fixed mounting of horizontal frame 52 inner wall bottom has driving motor two 54, be provided with belt pulley transmission structure 55 between driving motor two 54's the drive end and the left installation axle 57, the top and the equal fixed mounting in the place ahead that just is located two electromagnets 59 of belt transmission structure 56 inner wall have sand grip spacing groove 58.
The belt wheel transmission structure 55 is composed of two belt wheels and a belt, the two belt wheels are connected through belt transmission, the belt transmission structure 56 is composed of two gear discs and a transmission belt, and the two gear discs are connected through transmission belt transmission.
Seal structure 6 is including falling L shape frame 60, and the sliding hole sliding connection that falls L shape frame 60 was seted up through its top has vertical pole 62 rather than the looks adaptation, and vertical pole 62's quantity has two, the top fixedly connected with dog 64 of vertical pole 62, the sealed piece 61 of bottom fixedly connected with of vertical pole 62, the outer wall of vertical pole 62 just is located to cup joint between sealed piece 61 and the frame 60 of falling L shape and installs spring 63.
One side of the bottom of the Z-axis lifting plate 25 is fixedly provided with a first driving motor 24, the driving end of the first driving motor 24 is fixedly provided with a cross block 26, the bottom of the vertical shaft 39 is fixedly provided with a clamping seat 40, and the bottom of the clamping seat 40 is provided with a cross groove matched with the cross block 26.
The working principle of the invention is as follows:
when in use, when the culture dish is placed, the Z-axis motor 14 drives the Z-axis screw rod 11 to rotate, under the vertical transmission action of the Z-axis lead screw 11 and the Z-axis base plate 19, the Z-axis base plate 19 moves upwards, then the Z-axis lifting plate 25 will extend to the outside of the incubator body 1 through the culture dish port 10, at which time the vacuum suction cup 27 is exposed, the culture assembly 9 is placed at the suction port of the vacuum suction cup 27, then the vacuum pump 16 is started to generate negative pressure, the culture assembly 9 is fixed in an adsorption way through the adsorption pipeline 29, the metal hose 28 and the vacuum suction cup 27 in sequence, the culture assembly 9 is fixed in a vacuum adsorption type fixing way, the culture assembly 9 is convenient to absorb and place, and the five vacuum suction cups 27 are arranged, the five culture assemblies 9 can be adsorbed at the same time, so that the workload at the same time is greatly increased, and the efficiency is improved;
after the culture assembly 9 is completely adsorbed, the Z-axis substrate 19 is lowered into the incubator body 1, the magnet strip 32 is aligned with the channel 53 on the transverse frame 52 at the current height, the electric telescopic rod I17 is extended to drive the magnet mounting block 18 to move to the left, the electromagnet I34 and the iron block at the bottom of the Z-axis moving block 22 are magnetically adsorbed, so that the magnet strip 32 enters the channel 53, the convex strip 31 enters the convex strip limiting groove 58 to slide, then the electromagnet I34 is closed to remove the magnetism of the iron block at the bottom of the Z-axis moving block 22, meanwhile, the electromagnet II 59 is opened to carry out magnetic adsorption with the magnet strip 32, the driving motor II 54 drives the left mounting shaft 57 to rotate through the belt pulley transmission structure 55 to drive the belt transmission structure 56 to rotate, so that the electromagnet II 59 carries out transverse movement, and the electromagnet II 59 carries out magnetism with the strip 32, therefore, the Z-axis moving block 22 is driven to transversely suspend, the Z-axis moving block 22 can be moved in a vertical and transverse manner, the culture assembly 9 can be taken or placed at different heights and positions, and the practicability is high;
when the culture assembly 9 is positioned right below the vessel placing hole 42, the electric telescopic rod II 23 extends to drive the Z-axis lifting plate 25 to move upwards, the cross block 26 firstly enters the cross groove at the bottom of the clamping seat 40 to be clamped, then the driving motor I24 drives the cross block 26 to rotate so as to drive the vertical shaft 39 to rotate, under the transmission action of the gear transmission structure 44, the bidirectional threaded rod 43 rotates along with the vertical shaft 39, under the transverse transmission action of the two movable plates 46 and the bidirectional threaded rod 43, the two movable plates 46 move towards two sides to contact with the blocking of the vessel placing block 47 on the vessel placing hole 42 at the bottom, then the electric telescopic rod II 23 continuously extends to enable the culture assembly 9 to enter the interior of the vessel placing hole 42, the driving motor I24 rotates reversely, the two vessel placing blocks 47 reset and limit and fix the culture assembly 9, and complete the placing of the culture assembly 9, on the contrary, in the same way, the culture assembly 9 can be taken away and conveyed out of the interior of the incubator body 1 through the culture dish port 10, the traditional manual placing mode is changed, manual operation is driven by mechanical automation, and when the culture assembly 9 is placed or taken out, the opening time of the incubator body 1 is effectively shortened, so that the condition that the culture environment inside the incubator body 1 is changed and damaged due to the opening time process of the incubator body 1 is avoided, the possibility that the sample inside the incubator body 1 is damaged is reduced, and the successful culture probability of the incubator body 1 is improved;
when taking out cultivation subassembly 9, the nose bar 51 on the cultivation subassembly 9 can contact with the bottom of sealed piece 61, and upwards ejecting with it, sealed piece 61 can compress spring 63 this moment, the dog 64 is pulled to the hand upwards and can be carried out interim unsettled prevention to sealed piece 61, culture dish opening 10 is blockked by sealed piece 61 on seal structure 6, the influence that external environment caused the environment inside incubator body 1 has further been reduced, it is effectual to the inside environmental protection of incubator body 1, incubator body 1's result of use is good.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.

Claims (10)

1. The utility model provides a biological stem cell is culture dish and supporting incubator thereof, includes incubator body (1), its characterized in that: a box door (2) is arranged on one positive side of the incubator body (1), a control screen (5) is inlaid above the other positive side of the incubator body (1), a control button (4) is fixedly arranged below the other positive side of the incubator body (1), a bottom box (3) is welded and arranged at the bottom of the incubator body (1), six transverse moving structures (7) and six culture dish placing frames (8) are fixedly arranged on one side of the incubator body (1) at equal intervals, a culture assembly (9) is arranged in a placing cavity of each culture dish placing frame (8), an access opening (12) is formed in the other side of the bottom of the incubator body (1) in a penetrating mode, five culture dish openings (10) are formed in the other side of the top of the incubator body (1) in a penetrating mode, and the vertical section of each culture dish opening (10) is T-shaped, the incubator comprises an incubator body (1), five sealing structures (6) are fixedly mounted at the top of the incubator body (1) and located right above a culture dish through hole (10), two Z-axis sliding rods (15) are fixedly mounted between the bottom of the inner wall of the incubator body (3) and the top of the inner wall of the incubator body (1), a Z-axis motor (14) is fixedly mounted on one side of the bottom of the inner wall of the incubator body (3), a Z-axis lead screw (11) is fixedly connected to a driving end of the Z-axis motor (14), a Z-axis moving structure (13) is connected between the two Z-axis sliding rods (15) and one Z-axis lead screw (11) in a transmission mode, the Z-axis moving structure (13) comprises a Z-axis base plate (19), five Z-axis moving blocks (22) are slidably mounted on the top of the Z-axis base plate (19), a connecting welding plate (33) is fixedly connected between every two adjacent Z-axis moving blocks (22), and a magnet strip (32) is fixedly mounted on the side edge of one Z-axis moving block (22), the magnetic iron comprises a magnet strip (32), convex strips (31) are fixedly mounted at the top and the bottom of the magnet strip (32), two movable cavities (35) are formed in one side of a Z-axis base plate (19), electric telescopic rods (23) are fixedly mounted on two sides of the top of a Z-axis moving block (22), a Z-axis lifting plate (25) is fixedly connected with the movable ends of the electric telescopic rods (23), a vacuum chuck (27) is installed at the top of the Z-axis lifting plate (25) in an inserting mode, a metal hose (28) is fixedly connected with the suction end of the vacuum chuck (27), a vacuum pump (16) is fixedly mounted in the middle of one side of the bottom of the Z-axis base plate (19), an adsorption pipeline (29) is fixedly connected with the suction end of the vacuum pump (16), the suction end of the adsorption pipeline (29) is fixedly connected with the suction end of the metal hose (28), and an electromagnetic valve (30) is fixedly mounted at the joint of the adsorption pipeline (29) and the adsorption pipeline (29), the bottom of the Z-axis base plate (19) is fixedly provided with two electric telescopic rods I (17), the movable ends of the electric telescopic rods I (17) are fixedly connected with magnetic block mounting blocks (18), the tops of the magnetic block mounting blocks (18) are fixedly provided with electromagnets I (34), and the bottoms of the Z-axis moving blocks (22) are embedded with iron blocks.
2. The culture dish and the matched incubator thereof for the biological stem cell culture according to claim 1, wherein: subassembly (37) is placed including a curb plate (36) and five household utensils to culture dish rack (8), and curb plate (36) and the household utensils of leftmost side place subassembly (37) fixed connection, adjacent two the equal fixedly connected with middle part connecting block (38) of opposite face that subassembly (37) was placed to the household utensils.
3. The culture dish and the matched incubator thereof for the biological stem cell culture according to claim 2, wherein: subassembly (37) is placed to household utensils includes that the household utensils place board (41), the top and the top that the household utensils placed board (41) all run through and have seted up five household utensils and place hole (42), fixed mounting has five guide arms (48) between the both sides of board (41) inner wall are placed to the household utensils, the household utensils are placed and are rotated through the bearing between the both sides of board (41) inside and install five two-way threaded rod (43), and are adjacent transmission mounting has two fly leaf (46) between guide arm (48) and two-way threaded rod (43), two the equal fixed mounting of opposite face of fly leaf (46) has the household utensils to place piece (47), the household utensils are placed board (41) and are rotated through the bearing of its bottom mosaic installation and are installed vertical axis (39), be provided with gear drive structure (44) between the top of vertical axis (39) and two-way threaded rod (43).
4. The culture dish and the matched incubator thereof for the biological stem cell culture according to claim 3, wherein: one side of the movable plate (46) is respectively provided with a threaded hole and a sliding hole in a penetrating mode, the movable plate (46) is in sliding connection with the guide rod (48) through the sliding hole, the sliding hole is in sliding connection with the guide rod (48), the movable plate (46) is in transmission connection with the bidirectional threaded rod (43) through the threaded hole, the bidirectional threaded rod (43) is matched with the threaded hole, the two movable plates (46) move in the direction of simultaneous believed or reverse movement, the top of the vessel placing block (47) is provided with a placing groove (45), the gear transmission structure (44) is composed of two bevel gears which are matched with each other, and the two bevel gears are meshed with each other.
5. The culture dish and the matched incubator thereof for the biological stem cell culture according to claim 1, wherein: the top of the Z-axis base plate (19) is fixedly provided with five groups of Z-axis slide rails (21), the number of each group of Z-axis slide rails (21) is two, the bottom of each Z-axis moving block (22) is fixedly provided with two Z-axis moving block sliders (20) matched with the Z-axis slide rails (21), the top of the Z-axis base plate (19) is provided with two sliding holes matched with the Z-axis slide rails (15), the Z-axis base plate (19) is in sliding connection with the Z-axis slide rails (15) through the sliding holes, the top of the Z-axis base plate (19) is provided with threaded holes matched with the Z-axis lead screws (11), and the Z-axis base plate (19) is in transmission connection with the Z-axis lead screws (11) through the threaded holes.
6. The culture dish and the matched incubator thereof for the biological stem cell culture according to claim 1, wherein: the culture assembly (9) comprises a culture dish main body (49), a culture dish cover (50) is arranged at the top of the culture dish main body (49), a convex rod (51) is fixedly arranged at the top of the culture dish cover (50), and the top of the convex rod (51) is in a round table shape.
7. The culture dish and the matched incubator thereof for the biological stem cell culture according to claim 1, wherein: lateral shifting structure (7) are including horizontal frame (52), passageway (53) have been seted up in the front of horizontal frame (52) the both sides of horizontal frame (52) inner wall bottom all rotate and install installation axle (57), two the transmission is connected with belt drive structure (56) between installation axle (57), the conveyer belt outer wall fixed mounting of belt drive structure (56) has electromagnet two (59), one side fixed mounting of horizontal frame (52) inner wall bottom has driving motor two (54), be provided with belt pulley drive structure (55) between the drive end of driving motor two (54) and left installation axle (57), the top and the bottom of belt drive structure (56) inner wall just are located the equal fixed mounting in the place ahead of electromagnet two (59) and have sand grip spacing groove (58).
8. The culture dish and the complete incubator thereof for culturing the biological stem cells according to claim 7, wherein: the belt wheel transmission structure (55) is composed of two belt wheels and a belt, the two belt wheels are connected through belt transmission, the belt transmission structure (56) is composed of two gear discs and a transmission belt, and the two gear discs are connected through transmission belt transmission.
9. The culture dish and the matched incubator thereof for the biological stem cell culture according to claim 1, wherein: seal structure (6) are including falling L shape frame (60), the sliding hole sliding connection that falls L shape frame (60) was seted up through its top has vertical pole (62) rather than the looks adaptation, the top fixedly connected with dog (64) of vertical pole (62), the bottom fixedly connected with of vertical pole (62) seals piece (61), the outer wall of vertical pole (62) just is located to cup joint between sealed piece (61) and the frame (60) of falling L shape and installs spring (63).
10. The culture dish and the matched incubator thereof for the biological stem cell culture according to claim 1, wherein: one side fixed mounting of Z axle lifter plate (25) bottom has driving motor (24), the driving end fixed mounting of driving motor (24) has cross piece (26), the bottom fixed mounting of vertical axis (39) has cassette (40), and the bottom of cassette (40) is seted up the cross recess with cross piece (26) looks adaptation.
CN202110905263.7A 2021-08-09 2021-08-09 Culture dish for biological stem cell culture and matched incubator thereof Withdrawn CN113564047A (en)

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CN202110905263.7A CN113564047A (en) 2021-08-09 2021-08-09 Culture dish for biological stem cell culture and matched incubator thereof

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Application Number Priority Date Filing Date Title
CN202110905263.7A CN113564047A (en) 2021-08-09 2021-08-09 Culture dish for biological stem cell culture and matched incubator thereof

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116656496A (en) * 2023-07-28 2023-08-29 中国水产科学研究院黄海水产研究所 Marine phage culture device
CN117264766A (en) * 2023-11-17 2023-12-22 黑龙江中医药大学 Cell culture device for medical research

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116656496A (en) * 2023-07-28 2023-08-29 中国水产科学研究院黄海水产研究所 Marine phage culture device
CN116656496B (en) * 2023-07-28 2023-10-27 中国水产科学研究院黄海水产研究所 Marine phage culture device
CN117264766A (en) * 2023-11-17 2023-12-22 黑龙江中医药大学 Cell culture device for medical research
CN117264766B (en) * 2023-11-17 2024-02-06 黑龙江中医药大学 Cell culture device for medical research

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