CN217089023U

CN217089023U - Plant seed cultivates screening integrated device

Info

Publication number: CN217089023U
Application number: CN202122244406.2U
Authority: CN
Inventors: 于佳; 翁亚溟; 管皓羽
Original assignee: Taiyuan University of Science and Technology
Current assignee: Taiyuan University of Science and Technology
Priority date: 2021-09-16
Filing date: 2021-09-16
Publication date: 2022-08-02
Anticipated expiration: 2031-09-16

Abstract

The utility model relates to a plant seed cultivates screening integrated device belongs to seed cultivation experiment technical field, specifically include and be provided with the switch door on the aseptic incubator, install the baffle in the aseptic incubator, the culture dish is installed to the baffle and the box is collected, vacuum tank and vacuum pump are installed to the bottom of baffle, ultraviolet fluorescent lamp and aseptic ventilative filter are installed respectively to the lateral part of culture dish, translation mechanism is still installed at the top of aseptic incubator, install electric putter in the translation mechanism, install horizontal fixed plate on electric putter's the piston rod, install the negative pressure seed suction pipe on the horizontal fixed plate, the negative pressure seed suction pipe is connected with the vacuum tank through the connecting pipe, install the control valve on the connecting pipe, the controller is still installed to the outside of aseptic incubator, the utility model discloses simple structure, reasonable in design, low cost can carry out plant seed and callus under aseptic condition and cultivate and visual fluorescence screening.

Description

Plant seed cultivates screening integrated device

Technical Field

The utility model relates to a screening integrated device is cultivated to plant seed belongs to seed cultivation experiment technical field.

Background

A plant expression vector is constructed by utilizing a fluorescent protein coding gene, and a transgenic plant is obtained by transforming a plant by an agrobacterium-mediated method, which is one of the commonly used methods for functional gene research at present. Fluorescence is excited by an ultraviolet light source, phenotype analysis and screening of transgenic offspring can be rapidly carried out, and screening efficiency of homozygotes is effectively improved. For the screening of plant seeds or plant calluses which need to be cultured in an aseptic environment, the culture dish needs to be repeatedly opened to remove non-positive plants or tissues, so that the risk of pollution of bacteria, fungi and the like is increased, and the difficulty of obtaining transgenic positive homozygous plants is increased.

SUMMERY OF THE UTILITY MODEL

For solving the technical problem that prior art exists, the utility model provides a simple structure, reasonable in design, low cost can carry out plant seeds and callus under aseptic condition and cultivate and screen integrated device with plant seeds who visual fluorescence screening.

In order to achieve the above purpose, the technical scheme adopted by the utility model is an integrated plant seed cultivation and screening device, which comprises an aseptic incubator, wherein the aseptic incubator is provided with a switch door, the aseptic incubator and the switch door are made of light-transmitting materials, a baffle is installed in the aseptic incubator, a culture dish and a collection box are installed on the baffle, a vacuum tank and a vacuum pump are installed at the bottom of the baffle, an ultraviolet fluorescent lamp and an aseptic air-permeable filter are respectively installed at the lateral part of the culture dish, the ultraviolet fluorescent lamp and the aseptic air-permeable filter are respectively installed on the box wall of the aseptic incubator, a translation mechanism is also installed at the top of the aseptic incubator, an electric push rod is installed on the translation mechanism, a horizontal fixed plate is installed on the piston rod of the electric push rod, a negative pressure seed suction pipe is installed on the horizontal fixed plate, and the negative pressure seed suction pipe is connected with the vacuum tank through a connecting pipe, the connecting pipe is provided with a control valve, the outside of the sterile incubator is also provided with a controller, and the vacuum pump, the ultraviolet fluorescent lamp, the translation mechanism, the electric push rod and the control valve are respectively connected with the controller.

Preferably, the translation mechanism mainly comprises a transverse moving component and a longitudinal moving component, the transverse moving component is mounted on the longitudinal moving component, and the electric push rod is mounted on the transverse moving component.

Preferably, the longitudinal moving assembly comprises a first guide rod, first synchronizing wheels and rotating shafts, two rotating shafts are respectively installed on two sides of the top of the sterile incubator, the first synchronizing wheels are respectively installed at two ends of each rotating shaft, the first synchronizing wheels between the two rotating shafts are connected through a first synchronizing belt, a first servo motor is installed at the end of one rotating shaft, the first servo motor is installed on the outer wall of the sterile incubator, first guide rods are respectively arranged at two ends of the two rotating shafts, and two ends of each first guide rod are respectively installed on the sterile incubator;

the transverse moving assembly comprises sliders, second guide rods and second synchronous wheels, the sliders are respectively installed on the first guide rods, the two second guide rods are installed between the sliders, the two ends of the second guide rods are respectively fixed to the first synchronous belt through first connecting blocks, the second synchronous wheels are installed on the sliders, second synchronous belts are installed between the second synchronous wheels, one of the second synchronous belts is connected to the second synchronous wheels, the second servo motors are fixed to the sliders, the two guide rods are provided with fixing plates, the two ends of each fixing plate are respectively installed on the second guide rods through guide sleeves, the electric push rods are installed on the fixing plates, and the cylinder bodies of the electric push rods are installed on the second synchronous belts through second connecting blocks.

Preferably, one side of the partition plate is provided with a U-shaped clamping groove, the two sides of the collecting box are provided with folded edges, the collecting box is clamped in the U-shaped clamping groove, and the folded edges are located above the U-shaped clamping groove.

Preferably, the outside of aseptic incubator still installs the mount, infrared remote controller has been placed on the mount, infrared remote controller is connected with the controller.

Compared with the prior art, the utility model discloses following technological effect has: the utility model discloses simple structure, convenient to use places the culture dish that can carry out the seed cultivation in aseptic incubator, then utilizes ultraviolet fluorescence lamp to shine the seed, discern the seed that can send fluorescence, the transgene seed, rethread translation mechanism, electric putter control negative pressure seed sucker carries out the removal all around, about, from top to bottom, make negative pressure seed sucker aim at the transgene seed, rethread negative pressure jar produces the negative pressure at last, suck up the transgene seed, cut off negative pressure jar negative pressure and disappear, the seed drops in the collection box, whole process is gone on in aseptic incubator, need not hand contact seed to carry out the screening of seed, easy operation, convenient to use; and the cost is low.

In addition, the plant seed cultivation and screening device combining sterile culture and fluorescence screening can realize high-efficiency screening efficiency, effectively reduce pollution and improve the survival efficiency of positive plants so as to meet the requirements of safe, high-efficiency and quick screening of transgenic plant seeds.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic diagram of the internal structure of the present invention.

Fig. 3 is a schematic structural view of the middle translation mechanism of the present invention.

Fig. 4 is a partially enlarged view of a portion a in fig. 2.

Detailed Description

In order to make the technical problem, technical solution and advantageous effects to be solved by the present invention more clearly understood, the following description is given in conjunction with the accompanying drawings and embodiments to illustrate the present invention in further detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

As shown in fig. 1 to 4, the plant seed cultivation and screening integrated device comprises an aseptic incubator 1, a switch door 2 is arranged on the aseptic incubator 1, the aseptic incubator 1 and the switch door 2 are made of transparent materials, a partition plate 3 is arranged in the aseptic incubator 1, a culture dish 4 and a collection box 5 are arranged on the partition plate 3, a vacuum tank 6 and a vacuum pump 7 are arranged at the bottom of the partition plate 3, an ultraviolet fluorescent lamp 8 and an aseptic air-permeable filter 9 are respectively arranged at the side part of the culture dish 4, the ultraviolet fluorescent lamp 8 and the aseptic air-permeable filter 9 are respectively arranged on the box wall of the aseptic incubator 1, a translation mechanism is also arranged at the top of the aseptic incubator 1, an electric push rod 10 is arranged on the translation mechanism, a horizontal fixing plate 11 is arranged on a piston rod of the electric push rod 10, a negative pressure seed suction pipe 12 is arranged on the horizontal fixing plate 11, the negative pressure seed suction pipe 12 is connected with the vacuum tank 6 through a connecting pipe 13, the connecting pipe 13 is provided with a control valve 14, the exterior of the sterile incubator 1 is also provided with a controller 15, and the vacuum pump 7, the ultraviolet fluorescent lamp 8, the translation mechanism, the electric push rod 10 and the control valve 14 are respectively connected with the controller 15.

The utility model discloses a carry out seed cultivation and screening in the aseptic incubator of relative confined. After the aseptic incubator is subjected to aseptic treatment, the seeds are placed in a culture dish filled with a culture medium, and then the opening and closing door is closed. Then open the ultraviolet fluorescent lamp through the controller, under the irradiation of ultraviolet fluorescent lamp, fluorescence can appear in the transgenic seed in the culture dish, then through controller control translation mechanism, make electric putter just in time be located the top of transgenic seed, then stretch out through control electric putter again, aim at the seed with negative pressure seed suction pipe, then start the vacuum pump, open the control valve, negative pressure seed suction pipe produces the negative pressure, suck up the seed, then rethread translation mechanism removes the seed to the top of collecting the box, close the control valve, the seed falls down automatically. The steps are repeated until the seeds are completely screened. And finally, turning off the ultraviolet fluorescent lamp to carry out seed cultivation.

The translation mechanism mainly comprises a transverse moving component and a longitudinal moving component, the transverse moving component is installed on the longitudinal moving component, and the electric push rod is installed on the transverse moving component. The longitudinal moving assembly comprises a first guide rod 16, a first synchronizing wheel 17 and rotating shafts 18, two rotating shafts 18 are respectively installed on two sides of the top of the sterile incubator 1, the first synchronizing wheel 17 is installed on two ends of each rotating shaft 18, the first synchronizing wheel 17 between the two rotating shafts 18 is connected through a first synchronizing belt 19, a first servo motor 20 is installed at the end of one rotating shaft 18, the first servo motor 20 is installed on the outer wall of the sterile incubator 1, the first guide rods 16 are respectively arranged on two ends of the two rotating shafts 18, and two ends of each first guide rod 16 are respectively installed on the sterile incubator 1;

the transverse moving assembly comprises sliders 21, second guide rods 22 and second synchronous wheels 23, the two sliders 21 are respectively installed on the first guide rods 16, two second guide rods 22 are installed between the two sliders 21, two ends of the two second guide rods 22 are respectively fixed on the first synchronous belt 19 through first connecting blocks 33, the second synchronous wheels 23 are installed on the sliders 21, second synchronous belts 24 are installed between the second synchronous wheels 23, one of the second synchronous wheels 23 is connected with a second servo motor 25, the second servo motor 25 is fixed on the sliders, a fixing plate 26 is installed between the two second guide rods 22, two ends of the fixing plate 26 are respectively installed on the second guide rods 22 through guide sleeves 27, the electric push rod 10 is installed on the fixing plate 26, and a cylinder body of the electric push rod 10 is installed on the second synchronous belt 24 through the second connecting blocks 28.

The translation mechanism is composed of a transverse moving assembly and a longitudinal moving assembly, the transverse moving assembly drives a first synchronous wheel to rotate by using a first servo motor, and the longitudinal moving assembly can move on a first guide rod. The longitudinal moving assembly drives the second synchronizing wheel to rotate by adopting a second servo motor, so that the electric push rod can move along the second guide rod, and further, the transverse and longitudinal movement is realized.

In addition, one side of baffle 3 is provided with U type draw-in groove 29, and the both sides of collecting box 5 are provided with hem 30, collects box 5 and is used for the card in U type draw-in groove 29, and hem 30 is located U type draw-in groove 29's top, collects the box card in U type draw-in groove, and the dismouting of being convenient for is conveniently cleared up the seed. The outside of the sterile incubator 1 is also provided with a fixing frame 31, an infrared remote controller 32 is arranged on the fixing frame 31, and the infrared remote controller 32 is connected with the controller 15. The infrared remote controller can be connected with the controller to carry out remote control, observe the seed position, and it is more convenient to screen the seed.

The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. Plant seed cultivates screening integrated device, including aseptic incubator, be provided with the switch door on the aseptic incubator, its characterized in that: aseptic incubator and switch door are the printing opacity material and make, install the baffle in the aseptic incubator, the culture dish is installed to the baffle and the box is collected, vacuum tank and vacuum pump are installed to the bottom of baffle, ultraviolet fluorescent lamp and aseptic air filter are installed respectively to the lateral part of culture dish, ultraviolet fluorescent lamp and aseptic air filter install respectively on the tank wall of aseptic incubator, translation mechanism is still installed at the top of aseptic incubator, the last electric putter that installs of translation mechanism, install horizontal fixed plate on electric putter's the piston rod, install the negative pressure seed suction pipe on the horizontal fixed plate, the negative pressure seed suction pipe is connected with the vacuum tank through the connecting pipe, install the control valve on the connecting pipe, the controller is still installed to the outside of aseptic incubator, vacuum pump, ultraviolet fluorescent lamp, vacuum pump, aseptic, The translation mechanism, the electric push rod and the control valve are respectively connected with the controller.

2. The integrated plant seed cultivation and screening device of claim 1, wherein: the translation mechanism mainly comprises a transverse moving component and a longitudinal moving component, the transverse moving component is installed on the longitudinal moving component, and the electric push rod is installed on the transverse moving component.

3. The integrated plant seed cultivation and screening device of claim 2, wherein: the longitudinal moving assembly comprises a first guide rod, first synchronizing wheels and rotating shafts, two rotating shafts are respectively installed on two sides of the top of the sterile incubator, the first synchronizing wheels are respectively installed at two ends of each rotating shaft, the first synchronizing wheels between the two rotating shafts are connected through a first synchronizing belt, a first servo motor is installed at the end of one rotating shaft, the first servo motor is installed on the outer wall of the sterile incubator, first guide rods are further respectively arranged at two ends of the two rotating shafts, and two ends of each first guide rod are respectively installed on the sterile incubator;

4. The integrated plant seed cultivation and screening device according to any one of claims 1 to 3, wherein: one side of the partition plate is provided with a U-shaped clamping groove, the two sides of the collecting box are provided with folded edges, the collecting box is clamped in the U-shaped clamping groove, and the folded edges are located above the U-shaped clamping groove.

5. The integrated plant seed cultivation and screening device of claim 4, wherein: the outside of aseptic incubator still installs the mount, infrared remote controller has been placed on the mount, infrared remote controller is connected with the controller.