CN210157771U

CN210157771U - Umbrella fungus class fungal spore collection device

Info

Publication number: CN210157771U
Application number: CN201920840219.0U
Authority: CN
Inventors: 田孟华; 张昌飞; 胡德分; 丁培超; 杨新周; 余显伦
Original assignee: Zhaotong Tianma Research Institute
Current assignee: Zhaotong Tianma Research Institute
Priority date: 2019-06-05
Filing date: 2019-06-05
Publication date: 2020-03-20
Anticipated expiration: 2029-06-05

Abstract

The utility model discloses an umbrella fungus class fungal spore collection device, including base, toper endotheca, support, cage, the endotheca is embedded in the base of cask shape or support shape, the cage cover is detained on the base including the endotheca or the cover is detained in the outside of toper endotheca, support one end sets up in the bottom and the inside extension that the toper endotheca was passed to base and the other end, perhaps support one end is connected with the cage and the other end extends from the open end to the toper endotheca. The utility model discloses ingenious design a toper endotheca, the fungus sporocarp of umbellate fungus class passes through the support to be fixed in the toper endotheca, and the bottom of toper endotheca is concentrated to the natural whereabouts after the spore is ripe, conveniently collects, and supporting cover is detained in the cage of toper endotheca, can effectively keep apart the pollution of external miscellaneous fungus, and transparent or the cage that the top set up transparent filter window simultaneously can avoid wind to blow away tiny spore, and the ripe condition of the sporocarp of also being convenient for observe in addition to the adjustment spore acquisition time.

Description

Umbrella fungus class fungal spore collection device

Technical Field

The utility model belongs to the technical field of biology, concretely relates to simple structure, low cost, collect and separate convenient, the spore cleanliness factor and the high agaricus bisporus fungus class fungal spore collection device of rate of recovery.

Background

Fungi (A), (B)Fungus) Is a eukaryote. Common fungi are various types of mushrooms. Umbrella fungus (Agarics) Generally refers to a fungus having a fleshy umbrella-shaped fruiting body of pileus and stipe. Fruit body (fruiting body，sporocarp， fructification) Is the spore-forming structure of higher fungi, i.e. fruit body, and is composed of organized mycelium. Whether sexual reproduction or asexual reproduction, whether simple or complex in structure, the spore-forming structure is called a sporophore.

Rhizoma Gastrodiae (rhizoma Gastrodiae)Gastrodia elata Bl.) Is a rootless and leaf-like fungus which does not contain chlorophyll, does not have an organ for absorbing and producing nutrient substances, cannot be autotrophic, and normally depends on the digestion of Armillaria mellea (Armillariella mellea) invading into cortex and pericycle cellsArmillaria mellea (Vahl) P. Kumm.) Perennial herb plants of the genus Gastrodia of the family Orchidaceae grown with hypha as a nutrient source. The growth of Armillaria mellea can be divided into fruiting body and mycelium according to different stages, the mycelium is transformed into rhizomorph under adverse environmental conditions or after growth period, the rhizomorph grows under bark, and the fruiting body is formed under conditions of proper temperature and humidity. The sporophore is the main path of halimasch propagation, and the propagation and separation include two kinds of sporophore tissue culture separation and spore separation, although the spore separation has strong activity and germinationThe method has the advantage of high germination rate, but generally, because the quantity of spores obtained by each sporocarp is small, the spores are very small, the spores are difficult to identify by naked eyes, and the collection is difficult, the sporocarp tissue separation is the most common separation mode at present.

At present, the agaricus fungal spores are mainly collected by a bagging collection method, a spore printing collection method, an ejection collection method and the like. In the traditional spore print collection method and the bagging collection method, sporophores are in contact with collection paper or bags in the collection process, so that the cleanliness of the collected spores is difficult to ensure, the collected spores are difficult to transfer from the collection paper or bags, and the bagging collection method is not suitable for collecting the agaricus fungal spores because the collection environment is relatively closed and sporophores are easy to rot; according to the traditional ejection collection method, the fungal spores ejected on the ground are swept and collected, so that the obtained spores contain impurities such as silt and are easy to reduce in spore recovery rate due to wind due to open collection. Therefore, the collecting device which is simple in structure, low in cost, convenient and fast to separate and high in spore cleanliness and recovery rate is designed, not only can the spores of agaricus fungus spores, particularly the spores of armillaria sporophores be simply and reliably collected, but also the activity of the armillaria sporophores can be guaranteed, and the collecting device has important significance in promoting production of the gastrodia elata.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an umbrella fungus class fungal spore collection device simple structure, low cost, collect and separate convenient, spore cleanliness factor and rate of recovery height.

The purpose of the utility model is realized like this: the inner sleeve is embedded in a cylindrical or support-shaped base, the isolation cover is buckled on the base comprising the inner sleeve or on the outer side of the conical inner sleeve, one end of the support is arranged on the base, the other end of the support penetrates through the bottom of the conical inner sleeve and extends inwards, or one end of the support is connected with the isolation cover, and the other end of the support extends inwards from the opening end to the conical inner sleeve.

The utility model has the advantages that: the utility model skillfully designs a conical inner sleeve, the fruiting body of the agaricus fungus is fixed in the conical inner sleeve through the bracket, and the naturally falls and is concentrated at the bottom of the conical inner sleeve after the spores are mature, thereby being convenient for collection and transferring from the conical inner sleeve; and supporting cover is detained in the cage of toper endotheca, can effectively keep apart external miscellaneous fungus and atmospheric pollution, and transparent material or top set up transparent filter window's cage simultaneously can also avoid wind to blow away tiny spore to improve the rate of recovery of spore, also be convenient for observe the ripe condition of sporocarp moreover, so that adjust the effective acquisition time of spore, avoid rotten sporocarp pollution to collect the spore. Therefore, the utility model has the characteristics of simple structure, low cost, collect and separate convenient, spore cleanliness factor and rate of recovery height.

Drawings

FIG. 1 is a schematic view of the structure of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1;

FIG. 3 is a second schematic view of the structure of the utility model;

FIG. 4 is a third schematic view of the structure of the utility model;

FIG. 5 is a fourth schematic view of the structure of the utility model;

in the figure: 1-base, 2-conical inner sleeve, 201-opening, 202-sealing film, 3-support, 301-U-shaped fork, 302-supporting ring, 303-barb, 4-isolation cover, 401-through hole, 402-filtering window, 5-vibration device, 6-battery and 7-support frame.

Detailed Description

The present invention is further described with reference to the following drawings and examples, but the present invention is not limited thereto in any way, and any modification or improvement based on the teaching of the present invention is within the protection scope of the present invention.

As shown in fig. 1 to 5, the utility model discloses a base 1, toper endotheca 2, support 3, cage 4, endotheca 2 is embedded in base 1 of cask shape or support shape, cage 4 covers is detained on base 1 including endotheca 2 or the cover is detained in the outside of

toper endotheca

2, 3 one end of support sets up in base 1 and the other end passes the bottom of toper endotheca 2 and inwards extends, perhaps 3 one end of support is connected with cage 4 and the other end extends from the open end to toper endotheca 2.

The bottom of the conical inner sleeve 2 is provided with an opening 201, and the opening 201 is provided with a valve, an elastic plug or the outer side is provided with a detachable sealing film 202.

The elastic plug is a rubber plug, a silica gel plug or a cork plug.

One end of the bracket 3 is arranged on the base 1, and the other end of the bracket passes through the opening 201 and extends into the conical inner sleeve 2.

One end of the support 3 is hinged to the base 1 or directly placed on the surface of the base 1 and movably and hermetically connected with a valve on the opening 201 or a sealing film on the outer side, the part of the support 3 between the base 1 and the opening 201 is connected with the vibration device 5, and the vibration device 5 is electrically connected with the battery 6 or a power supply.

One end of the support 3 extending into the conical inner sleeve 2 is provided with a U-shaped fork 301 or a supporting ring 302.

The top end of the isolation cover 4 is provided with a through hole 401, and the support 3 is hung in the isolation cover 4 through the through hole 401; or one end of the bracket 3 can be movably hung in the isolation cover 4.

One side of the bracket 3 hung in the isolation cover 4, which is close to the isolation cover 4, is connected with a vibration device 5, and the vibration device 5 is electrically connected with a battery 6 or a power supply.

The support 3 is movably connected with the through hole 401 of the isolation cover 4 in a sealing mode, the vibration device 5 is connected to one side of the support 3 outside the isolation cover 4, and the vibration device 5 is electrically connected with the battery 6 or the power supply outside the isolation cover 4.

And one end of the bracket 3 extending towards the conical inner sleeve 2 is provided with a barb 303.

The isolation hood 4 is a transparent structure or is provided with a light-permeable and air-permeable filtering window 402 on the top.

The utility model discloses theory of operation and working process:

the utility model skillfully designs a conical inner sleeve, the fruiting body of the agaricus fungus is fixed in the conical inner sleeve through the bracket, and the naturally falls and is concentrated at the bottom of the conical inner sleeve after the spores are mature, thereby being convenient for collection and transferring from the conical inner sleeve; and the matching cover is buckled with the isolation cover of the conical inner sleeve, so that external mixed bacteria and atmospheric pollution can be effectively isolated, and fine spores can be prevented from being blown away by wind, so that the recovery rate of the spores is improved. Furthermore, the bottom of the conical inner sleeve is provided with an opening, and a valve is arranged on the opening or a detachable sealing film is arranged on the outer side of the opening; the bottom opening of the conical inner sleeve is beneficial to separating the collected spores from the opening and the inner wall of the conical inner sleeve, so that the spores are conveniently concentrated, and the contact surface between the spores and air is prevented from being too large and drifting during separation, thereby improving the recovery rate of the spores; and the valve on the opening or the sealing film on the outer side can not only avoid the external pollution during the collection, but also facilitate the separation after the collection. Furthermore, the support is arranged on the base, penetrates through the opening and extends into the conical inner sleeve, the support and the fruiting body fixed on the support are simple to install and operate, the whole structure is simple, and the cost is low. Furthermore, one end of the bracket is hinged with the base or is directly placed on the surface of the base and is movably and hermetically connected with the valve on the opening or the sealing film on the outer side, and the part of the bracket between the base and the opening is connected with a vibrating device; the vibration is transmitted to the fungus sporocarp through the bracket by the vibration device, so that the separation of the spores is promoted, the recovery rate of the spores is further improved, and the collection time of the spores is shortened. Furthermore, one end of the bracket extending towards the inner conical sleeve is provided with a U-shaped fork or a supporting ring, so that the fungus sporocarp can be fixed at the expected height without falling, and the collected spores can be prevented from falling and polluting after the sporocarp starts to become soft after being decomposed in the later period of collection. Further, the bracket is hung in the isolation cover through a through hole at the top end of the isolation cover, or one end of the bracket is movably hung in the isolation cover; the bracket penetrating through the through hole can facilitate the adjustment of the length of the bracket, and the external connection can not cause pollution to the inside of the isolation cover; the structure of the suspension in the isolation cover is simple. Furthermore, one side of the bracket hung in the isolation cover, which is close to the isolation cover, is connected with a vibration device, and the fungus sporocarp is vibrated by the vibration device, so that the separation of spores can be promoted, the recovery rate of the spores can be further improved, and the collection time of the spores can be shortened; the bracket is movably and hermetically connected with the through hole of the isolation cover, and one side of the bracket outside the isolation cover is connected with the vibration device; the recovery rate of the spores can be improved and the collection time of the spores can be shortened through the vibrating device, and the pollution in the inner isolation cover is avoided due to the external structure. Furthermore, one end of the support extending towards the conical inner sleeve is provided with a barb, so that the phenomenon that the fungus sporocarp falls down to pollute the collected spores due to fixation and fastness supplement can be effectively avoided. Furthermore, the isolation cover is of a transparent structure or the top of the isolation cover is provided with a light-permeable and ventilating filtering window, and the transparent structure or the light-permeable window can be used for conveniently observing the mature condition of the sporocarp so as to adjust the effective collection time of the spores and avoid the rotten sporocarp from polluting the collected spores; the ventilated filtering window is convenient for air exchange in the isolation hood, spores of the sporocarps can naturally mature and fall, so that the activity of the spores is effectively improved, the sporocarps can be prevented from being abnormally decayed and falling due to sealed air to pollute the collected spores, and the cleanliness of the spores is improved. To sum up, the utility model has the characteristics of simple structure, low cost, collect and separate convenient, spore cleanliness factor and rate of recovery height.

As shown in fig. 1 and fig. 2, taking collecting armillaria sporophore powder as an example, the base 1 is a cylindrical structure made of stainless steel and having a diameter of 8.1cm and a height of 3cm, one end of the cylindrical structure is sealed, and the other end is open, the conical inner sleeve 2 is nested in the base 1, the stainless steel needle-shaped support 3 passes through the opening 201 of the conical inner sleeve 2, and the bottom of the stainless steel needle-shaped support is directly placed at the bottom of the base 1; the isolation cover 4 is made of glass or transparent plastic into a cylindrical structure with the diameter of 8cm and the height of 15cm, and the isolation cover 4 covers and buckles the base 1 comprising the conical inner sleeve 2 so as to prevent the spores from being blown away by external pollution and wind during the collection of the spores.

Firstly, it is right the utility model discloses with the apparatus sterilize earlier, then carry out under aseptic condition and disinfect with 75% ethanol to the fruit body pileus surface and the stipe part of honey fungus to insert the fungus fold after the disinfection down on support 3 under aseptic condition, make the fruit body of honey fungus fix in the middle of the centre of toper endotheca 2, cover cage 4 on base 1, place in clean bench, the bottom of toper endotheca 2 is concentrated to the whereabouts of honey fungus spore after natural maturity, thereby collect the spore. The mature condition of the sporocarps is observed through the transparent isolation cover 4 or the filtering window 402 which is permeable to light and air of the isolation cover 4 until the collection of the spores is completed. When the spores fall into the bottom of the conical inner sleeve 2, the inner wall of the conical inner sleeve 2 is washed by sterile water to separate the spores to prepare a spore suspension, the spores are fully dispersed after being diluted step by step, and the spores are absorbed by a sterile suction pipe and then inoculated on a PDA plate culture medium. Culturing at 25 deg.C for about 3 days to give out a little white mycelium, picking out mycelium from the culture dish, transferring onto slant culture medium, and culturing for several days to give out mycelium.

Claims

1. The utility model provides an umbellate fungus class fungi spore collection device, its characterized in that includes base (1), toper endotheca (2), support (3), cage (4), endotheca (2) are embedded in base (1) of cask shape or support shape, cage (4) cover is detained on base (1) including endotheca (2) or the cover is detained in the outside of toper endotheca (2), support (3) one end sets up in the bottom and the inside extension of toper endotheca (2) are passed to base (1) and the other end, perhaps support (3) one end is connected with cage (4) and the other end extends to toper endotheca (2) from the open end.

2. The agaricus fungus spore collecting device according to claim 1, wherein the bottom of the conical inner sleeve (2) is provided with an opening (201), and the opening (201) is provided with a valve, an elastic plug or a detachable sealing film on the outer side.

3. The device for collecting fungal spores of agaricus as defined in claim 2, wherein the bracket (3) has one end disposed on the base (1) and the other end passing through the opening (201) and extending into the conical inner case (2).

4. The agaricus fungus spore collecting device according to claim 3, wherein one end of the support (3) is hinged with the base (1) or directly placed on the surface of the base (1) and is movably and hermetically connected with a valve on the opening (201) or an outer sealing film, the part of the support (3) between the base (1) and the opening (201) is connected with the vibrating device (5), and the vibrating device (5) is electrically connected with a battery (6) or a power supply.

5. The agaricus blazei murill spore collecting device according to claim 2, wherein one end of the bracket (3) extending into the conical inner sleeve (2) is provided with a U-shaped fork (301) or a supporting ring (302).

6. The agaricus fungus spore collecting device according to claim 2, wherein the top end of the isolation cover (4) is provided with a through hole (401), and the bracket (3) is hung in the isolation cover (4) through the through hole (401); or one end of the bracket (3) can be movably hung in the isolation cover (4).

7. The agaricus fungus spore collecting device according to claim 6, wherein the bracket (3) hung in the isolation hood (4) is connected with a vibration device (5) near one side of the isolation hood (4), and the vibration device (5) is electrically connected with a battery (6) or a power supply.

8. The agaricus fungus spore collecting device according to claim 6, wherein the bracket (3) is movably and hermetically connected with the through hole (401) of the isolation cover (4), the bracket (3) is connected with the vibrating device (5) at one side outside the isolation cover (4), and the vibrating device (5) is electrically connected with the battery (6) or the power supply outside the isolation cover (4).

9. The agaricus blazei murill spore collecting device according to claim 6, wherein the end of the bracket (3) extending into the conical inner sleeve (2) is provided with a barb (303).

10. The apparatus for collecting fungal spores of umbelliform fungi of any one of claims 1 to 9, characterized in that the shielding case (4) is a transparent structure or is provided with a light-permeable, air-permeable filtering window (402) at the top.