CN111616108A - Device integrating field portable collection and feeding of bemisia tabaci and using method thereof - Google Patents
Device integrating field portable collection and feeding of bemisia tabaci and using method thereof Download PDFInfo
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- CN111616108A CN111616108A CN202010615799.0A CN202010615799A CN111616108A CN 111616108 A CN111616108 A CN 111616108A CN 202010615799 A CN202010615799 A CN 202010615799A CN 111616108 A CN111616108 A CN 111616108A
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- 241000254127 Bemisia tabaci Species 0.000 title claims abstract description 103
- 238000000034 method Methods 0.000 title claims abstract description 42
- 230000006835 compression Effects 0.000 claims abstract description 49
- 238000007906 compression Methods 0.000 claims abstract description 49
- 230000000877 morphologic effect Effects 0.000 claims abstract description 10
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 74
- 241000208125 Nicotiana Species 0.000 claims description 57
- 241000238631 Hexapoda Species 0.000 claims description 37
- 241000196324 Embryophyta Species 0.000 claims description 30
- 238000007789 sealing Methods 0.000 claims description 23
- 241000258937 Hemiptera Species 0.000 claims description 18
- 244000061176 Nicotiana tabacum Species 0.000 claims description 18
- 239000002689 soil Substances 0.000 claims description 16
- 238000012360 testing method Methods 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 10
- 239000000877 Sex Attractant Substances 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 7
- 238000009395 breeding Methods 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 229920001903 high density polyethylene Polymers 0.000 claims description 3
- 239000004700 high-density polyethylene Substances 0.000 claims description 3
- 229920001684 low density polyethylene Polymers 0.000 claims description 3
- 239000004702 low-density polyethylene Substances 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 230000003071 parasitic effect Effects 0.000 claims description 2
- -1 polypropylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 229910001220 stainless steel Inorganic materials 0.000 claims description 2
- 239000010935 stainless steel Substances 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 239000012780 transparent material Substances 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 10
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C23/00—Distributing devices specially adapted for liquid manure or other fertilising liquid, including ammonia, e.g. transport tanks or sprinkling wagons
- A01C23/04—Distributing under pressure; Distributing mud; Adaptation of watering systems for fertilising-liquids
- A01C23/042—Adding fertiliser to watering systems
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G27/00—Self-acting watering devices, e.g. for flower-pots
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Water Supply & Treatment (AREA)
- Zoology (AREA)
- Insects & Arthropods (AREA)
- Wood Science & Technology (AREA)
- Soil Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to a field portable device for collecting and feeding bemisia tabaci and a using method thereof, wherein the device comprises two forms: a) the morphological structure of the collecting device and the morphological structure of the feeding device; the shape structure of the acquisition device comprises a breathable top cover, a compression tank and a breathable bottom cover, wherein the breathable top cover, the compression tank and the breathable bottom cover are sequentially connected in a clamping manner or in a threaded connection or a nested connection from top to bottom; the shape structure of the feeding device comprises a ventilating top cover, a compression tank and a hard tank, wherein the ventilating top cover, the compression tank and the hard tank are sequentially connected in a clamping sleeve manner or in a threaded connection or a nested connection from top to bottom; the device has the advantages of convenience in carrying, simplicity in operation, high flexibility, time and labor saving, and low manufacturing cost, can effectively combine the bemisia tabaci collecting and feeding devices into a whole, can provide great convenience for development of research work of controlling the bemisia tabaci pests, and can be popularized and used in the field.
Description
Technical Field
The invention belongs to the technical field of insect collecting device structures and collecting methods, and particularly relates to a portable collecting device for whitefly pests and a using method of the portable collecting device.
Background
Bemisia tabaci Bemisia gennadius also named as Bemisia gossypii and Bemisia tabaci, belongs to Alyrenodidae of Homoptera whitefly of Hemiptera, is widely distributed in 90 countries and regions of various continents except Antarctic all over the world, and is a worldwide pest. Now become important pests in countries such as the United states, India, Pakistan, Sudan and Israel, and bring serious economic loss to the global agricultural production. Since the beginning of the record in 1949, the outbreak in Guangdong, Beijing, Hebei, Tianjin, Xinjiang, Shanxi and the like in succession causes serious economic loss. The range of the Bemisia tabaci host plants is quite wide, according to preliminary statistics, more than 500 species of Bemisia tabaci are present in the hosts, the Bemisia tabaci is mainly harmful to crops, namely nymphs and adults of the Bemisia tabaci directly prick and absorb the juice of the crops, so that leaves of the plants turn yellow and even the plants die, and a large amount of plant viruses are indirectly transmitted and aggravated while the plants are pricked and absorbed, and more than 100 species of plant viruses which can be carried and transmitted by the Bemisia tabaci are reported at present, so that serious economic loss is.
In the control work of the whitefly pests, ecological investigation is necessary, and the information of whitefly species, population space distribution, occurrence quantity and the like is often investigated in different ecological systems in the field so as to adopt corresponding control strategies. Mainly utilize to gather insect cage and gather from valve bag in current collection technique, because there is bulky, carry defects such as inconvenient in gathering insect cage, the researcher in this field can select to use valve bag to gather mostly, but valve bag is airtight, lack the tightness in the transportation, cause serious damage to the polypide easily to valve bag belongs to disposable article more, its use easily causes wasting of resources and environmental pollution in a large number, uses valve bag to gather whitefly and still has a great deal of drawback. In addition, after the field collection is finished, subsequent indoor tests are needed, collected whitefly pests need to be raised indoors temporarily, and the problems of pest escape, pest body damage and the like exist when whiteflies are transferred to a separate raising device. Therefore, the invention research is carried out on the portable collecting and feeding device for the whitefly pests aiming at the problems.
Disclosure of Invention
The invention aims to overcome the defects of the existing collecting device and provides the bemisia tabaci collecting and feeding device which is reasonable in structure and convenient to carry and the using method.
The invention is realized by the following technical scheme.
A field portable device for collecting and feeding bemisia tabaci integrally comprises a breathable top cover 1, a compression tank 2, a breathable bottom cover 3 and a hard tank 4; the device comprises two forms formed by the four components: a) the morphological structure of the collecting device and the morphological structure of the feeding device;
the shape structure of the acquisition device comprises a ventilating top cover 1, a compression tank 2 and a ventilating bottom cover 3, wherein the ventilating top cover 1, the compression tank 2 and the ventilating bottom cover 3 are sequentially connected in a clamping sleeve manner or in a threaded connection or a nested connection from top to bottom;
the feeding device is characterized in that the shape structure of the feeding device comprises a ventilating top cover 1, a compression tank 2 and a hard tank 4, wherein the ventilating top cover 1, the compression tank 2 and the hard tank 4 are sequentially connected in a clamping or threaded or nested manner from top to bottom;
the breathable top cover 1 and the breathable bottom cover 3 comprise annular cover bodies, air holes arranged in the middle of the cover bodies, top cover gauze 1.1 and bottom cover gauze 3.1 arranged in the middle of the air holes;
the hard tank 4 is arranged as a tank body with an opening at the top, and the tank body is made of hard material;
the device still includes arc activity handle 1.2, arc activity handle 1.2 both ends respectively fixed the setting at the edge of top cap 1, with top cap 1 swing joint. The bottom cover 3 and the hard tank 4 are of alternative structures, the bottom cover 3 is replaced by a collecting device during insect collection, and the hard tank 4 is replaced by a feeding device during feeding; the collecting device and the feeding device can be flexibly converted and can be better matched for use.
Furthermore, the side wall of the hard tank 4 is provided with a pouring gate 5, the pouring gate 5 is fixedly sleeved with a finger-shaped pipe 5.1, and the outer end of the finger-shaped pipe 5.1 is provided with a plug filler. The pouring gate 5 arranged on the side wall of the hard tank 4 is formed by hollowing and sticking a finger-shaped pipe cover on the side wall of the tank and connecting the finger-shaped pipe cover with a finger-shaped pipe 5.1 through a rotating wire.
Furthermore, when the device adopts a morphological structure, the device also comprises a filter box 3.2 and a transparent sealing sheet 3.4 which are matched with the bottom cover 3; the filter box 3.2 is arranged as a vessel, the transparent sealing sheet 3.4 is covered on the filter box 3.2, and the bottom of the filter box 3.2 is provided with a filter box gauze 3.3;
furthermore, when the device is in a feeding device morphological structure, the device also comprises a conical gauze 4.1 and a hollow support sheet 4.2 which are matched with the hard tank 4; the hollow support sheet 4.2 is covered on the conical gauze 4.1, and the bottom of the conical gauze 4.1 is provided with a round base 4.3.
Furthermore, the upper opening of the compression tank 2 and the top cover 1, the lower opening of the compression tank 2 and the bottom cover 3, and the lower opening of the compression tank 2 and the hard tank 4 are connected through the arranged snap fasteners 6.
Furthermore, the tank body and the cover are made of transparent materials, so that the tank body and the cover can be observed from the outside conveniently; wherein the compression tank 2 is made of transparent low-density polyethylene LDPE material, the hard tank 4, the top cover 1, the bottom cover 3, the filter box 3.2, the transparent sealing sheet 3.4, the hollow supporting sheet 4.2 and the round base 4.3 are made of transparent high-density polyethylene HDPE material, and the pouring gate 5 and the finger-shaped pipe 5.1 are made of transparent polypropylene PP material; the top cover gauze 1.1, the bottom cover gauze 3.1 and the filter box gauze 3.2 are all made of nylon materials; the cone-shaped gauze 4.1 is made of stainless steel material.
Further, the device of the present invention has the following dimensional parameters: the inner diameter of the top cover 1 is 15 cm-20 cm, the thickness is 0.2cm, and the height is 1 cm; the distance between the top cover gauze and the top cover edge is 1.5cm, and the diameter is 12 cm-17 cm; the bottom cover 3 is consistent with the top cover 1 in size; the diameter of a large circle of the tank body of the compression tank 2 is 15 cm-20 cm, the diameter of a small circle is 12 cm-17 cm, the thickness is 0.01cm, the outer diameter of a connecting port of the compression tank 2 is 15 cm-20 cm, the height is 1cm, the thickness is 0.2cm, and the longest length of the compression tank 2 is extended to 30 cm; the thickness of the filter box is 3.2 cm, the outer diameter of the filter box is equal to the inner diameter of the connecting port of the compression tank 2, the thickness of the transparent sealing sheet is 3.4 cm, and the diameter of the transparent sealing sheet is 15 cm-20 cm; the inner diameter of the connecting port of the hard tank 4 is 15 cm-20 cm, the height is 1cm, the thickness is 0.2cm, the tank body of the hard tank 4 is 0.1cm, the diameter is 15 cm-20 cm, and the height is 10 cm; the diameter of the conical gauze is 4.1, the height of the conical gauze is 10cm, the diameter of the hollow support sheet is 15 cm-20 cm, the diameter of the hollow circle is 2cm, and the thickness of the hollow circle is 0.1 cm; the aperture of a finger-shaped pipe 5.1 matched with the pouring gate is 1.5 cm; the mesh number of the top cover gauze 1.1, the bottom cover gauze 3.1 and the filter box gauze 3.3 is 100-120 meshes, and the mesh number of the cone-shaped gauze 4.1 is 10 meshes.
By using the method of the device for field portable collection and breeding of the bemisia tabaci, the volume of the device can be greatly reduced, and the device is particularly convenient to carry or store when going out; the field collection comprises the following specific steps:
the method comprises the following steps: when insects are collected in an outward process, the collecting device is replaced, namely the hard tank 4 is replaced by the bottom cover 3, the filter box 3.2 and the transparent sealing sheet 3.4 are arranged, and the device is compressed to 1/7-1/8 of the height of the tank body, so that the carrying is convenient;
step two: when the device is used in a field, the bemisia tabaci sex attractant is placed in the filter box 3.2, the transparent sealing sheet 3.4 is covered to prevent the sex attractant from being diffused from the top cover 1, the bottom cover 3 is opened, the compressed tank 2 is stretched, and then young and tender bemisia tabaci host plants are placed in the tank, so that the attracted bemisia tabaci is attached to the host plants, and finally the device is taken as an attraction device and is hung on the host plants with bemisia tabaci populations through the arc-shaped movable handle 1.2;
step three: when the trapping device is collected, the artificially collected tobacco whitefly or the parasitic blades of the tobacco whitefly larvae are placed into the device, the bottom cover 3 is covered, the tobacco whitefly sex attractant and the transparent sealing sheet 3.4 are taken down, the device is used as a device for transportation and storage, and the volume of the tank is freely adjusted according to the number of collected samples; the device has good ventilation, can not cause extrusion, can not influence the normal activity of insects, and ensures the reliability of test data.
Furthermore, the device skillfully combines a field portable collecting device and the living plant together; the method comprises two methods of soil cultivation tobacco feeding and soilless cultivation tobacco feeding, and comprises the following specific steps:
the method comprises the following steps: soil cultivation of tobacco seedlings: transplanting tobacco seedlings in a seedling stage into the hard pot 4 serving as a carrier, cultivating the tobacco with soil, and feeding the tobacco whiteflies after the tobacco seedlings grow to a root extending stage;
soilless culture tobacco seedling raising: putting a proper amount of nutrient solution into a hard tank 4, putting a cone-shaped gauze 4.1 into the hard tank 4, putting tobacco seedlings cultivated without soil and a substrate into the bottom of the cone-shaped gauze 4.1, covering a hollow support sheet 4.2 to ensure that the tobacco seedlings extend out from a middle hole, and feeding the bemisia tabaci when the tobacco seedlings grow to a root extending period; for the whiteflies to be raised collected in the field, the bottom cover 3 of the collecting device is detached, the compression tank 2 and the collected whiteflies are directly sleeved on the hard tank 4 in which tobacco is planted in advance, and the snap fastener 6 is fastened, namely living plants are used for raising insects;
step two: opening the watering port 5 to add nutrient solution to the tobacco seedlings at proper time: if the tobacco begins to wither or die, the tobacco needs to be replaced to continue feeding, at the moment, the snap fastener 6 needs to be opened, the tobacco plant is cut, the cut tobacco plant and the compression tank are sleeved on the other hard tank 4 in which the healthy tobacco seedlings are planted, the snap fastener 6 is fastened, after the whitefly is completely transferred from the withered tobacco plant to the healthy tobacco plant, the snap fastener 6 is opened, the withered tobacco plant is drawn out, and the snap fastener 6 is fastened again, so that the replacement of the tobacco plant is completed;
step three: the growth condition of insects in the tank is observed through the transparent tank body: when the fed Bemisia tabaci needs to be taken for testing, the top cover is opened, the Bemisia tabaci is sucked by the insect suction pipe, and when the Bemisia tabaci at the lower part of the host plant needs to be sucked, the compression tank 2 is compressed until the Bemisia tabaci is exposed out of the host plant for sucking.
The invention has the beneficial effects that: a field portable collection of tobacco whitefly and raising device and method as an organic whole, adopt the above-mentioned structure and raising method, the apparatus stated in the invention includes field portable collection device and indoor raising device, two structures cooperate each other in use, supplement each other and use; wherein collection system has set up sex attractant and has lured the device, and structural design is convenient for deposit and carry, can compress the 1/8 to the jar height with the device to can adjust jar volume in a flexible way when using, solved traditional insect collecting cage bulky, carry inconvenient defect, and proclaim oneself the bag airtight, easily cause the damage to the polypide, defects such as wasting of resources and environmental pollution. On the other hand, the compression tank can be matched with a feeding device for use, and the devices can be tightly matched after being combined, so that the devices are prevented from falling; the living host plants can be used for feeding the bemisia tabaci, the complex operations of moisture preservation, food replacement and the like in the traditional feeding are solved, a natural environment beneficial to the growth and development of the bemisia tabaci is created, and two cultivation modes of soil cultivation and soilless cultivation are designed for the host plants; the watering port is arranged on the side wall of the hard tank, so that the tobacco seedlings can be conveniently supplemented with nutrient solution through the watering port, and the operation steps of uncovering and watering are simplified; and the physiological changes of the insects and the host plants in the pots can be clearly observed to ensure the orderly progress of the test. The device has the advantages of convenience in carrying, simplicity in operation, high flexibility, time and labor saving, and low manufacturing cost, can effectively combine the bemisia tabaci collecting and feeding devices into a whole, can provide great convenience for development of research work of controlling the bemisia tabaci pests, and can be popularized and used in the field.
The invention is further explained below with reference to the drawings and the detailed description.
Drawings
Fig. 1 is a schematic diagram of a field portable acquisition device of the invention.
FIG. 2 is a schematic view of the device for feeding bemisia tabaci by soil-cultivated tobacco seedlings.
FIG. 3 is a schematic diagram of the device for feeding bemisia tabaci by soilless culture tobacco seedlings.
Fig. 4 is a schematic view of the field portable collecting device of the present invention in a compressed state.
Fig. 5 is a schematic view of the connection mode of the field portable collecting device of the invention.
FIG. 6 is a schematic view of the connection mode of the tobacco seedlings cultivated in soil for feeding bemisia tabaci of the invention.
FIG. 7 is a schematic view of the connection mode of the soilless culture tobacco seedling for feeding bemisia tabaci.
The reference numbers in the figures are: the device comprises a top cover (1), a top cover gauze (1.1), a movable handle (1.2), a compression tank (2), a bottom cover (3), a bottom cover gauze (3.1), a filter box (3.2), a transparent sealing sheet (3.4), a hard tank (4), a conical gauze (4.1), a hollow supporting sheet (4.2), a circular base (4.3), a water pouring port (5), a finger-shaped pipe (5.1) and a snap fastener (6).
Detailed Description
In order that those skilled in the art will better understand the method of the present invention, the following detailed description will be given with reference to the accompanying drawings.
See fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7.
The invention discloses a device for integrally collecting and feeding bemisia tabaci outdoors, which comprises a portable field collecting device and an indoor feeding device, wherein the portable field collecting device comprises a main body and a main body, and the main body comprises a main body and a main body, wherein the main body comprises a main body and a main body, the main body comprises a main body, and the:
the portable field acquisition device is provided with a top cover 1, a top cover compression tank 2 and a bottom cover 3, wherein the middle part of the top cover is provided with a top cover gauze 1.1, the edge of the top cover is provided with a movable handle 1.2, and the middle part of the bottom cover is also provided with a bottom cover gauze 3.1. When not in use, the tank body can be compressed to 1/8 volume, thus reducing occupied space and being convenient for storage and carrying; when in use, the tank body is stretched, and the volume of the tank body can be freely adjusted according to the number of collected samples.
The indoor feeding device for bemisia tabaci has the advantages that only the bottom cover 3 needs to be replaced by the hard tank 4, the hard tank 4 is used as a carrier for planting host plant tobacco of bemisia tabaci, the compression tank 2 is connected with the hard tank 4 with the tobacco planted in advance through the snap fastener during use, the snap fastener 6 is fastened, the living plant can be used for feeding insects, and water and compound fertilizer which are necessary for growth can be added for the host plant from the watering opening.
The specific use steps and the method of the device integrating field portable collection and indoor feeding of the bemisia tabaci in the actual work comprise the following two parts:
the field acquisition portable device is characterized in that the device can greatly compress the volume of the device and is particularly convenient to carry or store when going out; the field collection comprises the following specific steps:
the method comprises the following steps: when insects are collected in an outward mode, the collecting device is replaced, namely the hard tank 4 is replaced by the bottom cover 3, and the device can be compressed to 1/7-1/8 of the height of the tank body, so that the device is convenient to carry;
step two: when in use, the collected insects or plant leaves can be placed only by stretching the compression tank 2 and then opening the top cover, and the volume of the tank can be freely adjusted according to the number of collected samples;
step three: this device ventilates well, can not cause the extrusion, gathers the normal activity that can not influence the insect with this device, and the insect that needs to handle after returning the laboratory can be to its direct processing.
The indoor feeding device is characterized in that the device skillfully combines a field portable collecting device and a living plant together; the breeding method comprises the following specific steps:
the method comprises the following steps: the tobacco as the host plant of bemisia tabaci is planted in the hard tank 4 as a carrier. For the whiteflies to be raised collected in the field, the bottom cover 3 of the collecting device can be detached, the compression tank 2 and the collected whiteflies are directly sleeved on the hard tank 4 with tobacco planted in advance, and the snap fastener 6 is fastened, so that the living plants can be used for raising insects;
step two: the watering opening 5 can be opened to add water and compound fertilizer necessary for the growth of the tobacco. If the tobacco begins to wither or die, the tobacco needs to be replaced to continue feeding, at the moment, the snap button 6 needs to be opened, the tobacco plant is cut, the cut tobacco plant and the compression tank are sleeved on the other hard tank 4 with healthy tobacco, the snap button 6 is fastened, after the whitefly is completely transferred from the withered tobacco plant to the healthy tobacco plant, the snap button 6 is opened, the withered tobacco plant is drawn out, and the snap button 6 is fastened again, so that the replacement of the tobacco plant is completed;
step three: the growth condition of insects in the tank can be observed through the transparent tank body. When the fed Bemisia tabaci needs to be taken for testing, the top cover is opened, and the Bemisia tabaci is sucked by the insect suction pipe, and when the Bemisia tabaci at the lower part of the host plant needs to be sucked, the compression tank 2 can be compressed until the Bemisia tabaci is exposed out of the host plant for sucking.
Specific examples are described in detail.
Specific operation process
The present invention will be further illustrated with reference to the following specific examples, which are carried out in detail on the basis of the technical solutions of the present invention, and it should be understood that these examples are only intended to illustrate the present invention and do not limit the scope of the present invention.
(1) Device structure
Briefly, the following steps are carried out: the device comprises a field collecting device and an indoor feeding device, wherein the collecting device and the feeding device can be connected and matched through an occlusion buckle for use; the field collecting device is characterized in that the main body of the field collecting device is a compression tank, the upper buckle and the lower buckle are both provided with covers connected through occlusion buckles, and the center of each cover is provided with a gauze for facilitating air circulation; the upper opening is also provided with a filter box for placing the sex attractant in use, and the filter box is covered with a transparent sealing sheet to prevent the sex attractant from diffusing and overflowing from the upper opening; the feeding device is provided with soil cultivation tobacco seedling feeding and soilless cultivation tobacco seedling feeding, wherein the soil cultivation device is a hard tank which is connected with the lower opening of the compression tank through an occlusion buckle and is used for containing the soil cultivation tobacco seedlings, and the side wall of the soil cultivation device is provided with a watering opening for adding nutrient solution to the tobacco seedlings; the soilless culture device is characterized in that a conical gauze used for attaching the root system of the tobacco seedling and a hollow support sheet used for fixing the stem of the tobacco seedling are added on the basis of the soil culture device.
(1) Device specification
The relevant specifications of the device of the invention are: the inner diameter of the top cover 1 is 20cm, the thickness is 0.2cm, and the height is 1 cm; the top cover gauze is 17 cm; the bottom cover 3 is consistent with the top cover 1 in size; the diameter of the big circle of the compression tank 2 is 20cm, the diameter of the small circle is 17cm, the thickness is 0.01cm, and the longest length of the compression tank 2 can extend to 30 cm; the outer diameter of a connecting port of the compression tank 2 is 20cm, the height is 1cm, and the thickness is 0.2 cm; the thickness of the filter box is 3.2 cm, the outer diameter is 19.8cm, the thickness of the transparent sealing sheet is 3.4 cm, and the diameter is 20 cm; the diameter of the connecting opening of the hard tank 4 is 20cm, the height of the connecting opening is 1cm, the thickness of the connecting opening is 0.2cm, and the thickness of the tank body of the hard tank 4 is 0.1cm, the diameter of the connecting opening is 20cm, and the height of the connecting opening is 10 cm; the diameter of the conical gauze is 4.1, the height of the conical gauze is 10cm, the diameter of the hollow support sheet is 20cm, the diameter of the hollow circle is 2cm, and the thickness of the hollow circle is 0.1 cm; the aperture of a finger-shaped pipe 5.1 matched with the pouring gate is 1.5 cm; the mesh number of the top cover gauze 1.1, the bottom cover gauze 3.1 and the filter box gauze 3.3 is 100-120 meshes, and the mesh number of the cone-shaped gauze 4.1 is 10 meshes.
(2) Insect source for test and test
Collecting and feeding test of the bemisia tabaci adults: the method comprises the following steps that bemisia tabaci on tomato leaves in different planting areas is selected in a test, wherein the bemisia tabaci comprises a flower planting area, a riverside planting area and a mountain planting area, and in the test, the bemisia tabaci is collected on tomatoes planted in the three planting areas by using the method, and living tobacco seedlings are raised by using the device; in addition, yellow board trapping is adopted during collection, and a tobacco branch water cutting method is also adopted during feeding.
Test one: 3 sample areas of each planting area are selected by a system for investigation, the densities of different insect states of bemisia tabaci on tomato leaves planted in a flower planting area, a riverside planting area and a mountain planting area are obtained, 3 land blocks of the 3 planting areas are randomly collected during investigation and collection, each land block is collected by a five-point sampling method, 20 tomato leaves are collected at each point, the number of the bemisia tabaci is recorded and the leaves are picked off when the tomato leaves are turned over, the collected leaves are respectively placed in the device, the insect collecting cage and the self-sealing bag for storage, are transported back to a laboratory for subsequent breeding tests, and are used for comparing the transportation effects of different storage modes at the later stage.
And (2) test II: comparing the trapping efficiency of the device trapping and the yellow board trapping, and comparing the preparation time of the two methods to obtain the labor efficiency; the device of the invention is compared with physical characteristics such as collecting and transporting effects, morphological characteristics and the like of a yellow plate, an insect collecting cage and a self-sealing bag.
Experiment three: the soilless culture and soil culture device and the traditional branch water culture and air survival 4 method are respectively adopted to carry out culture tests on the bemisia tabaci, the phenomena of incubation, survival, emergence and the like of the bemisia tabaci cultured by different methods for feeding the bemisia tabaci are observed, the data of the incubation rate, the survival rate, the emergence rate, the water adding and moisture retention times, the tobacco seedling replacing times and the like of the bemisia tabaci fed by different devices are counted, and the feeding efficiency of the different devices is analyzed.
(3) Bemisia tabaci density on tomatoes in 3 different planting areas
TABLE 13 comparison of Bemisia tabaci Density on tomatoes of different planting areas
Note: the values in the table are mean. + -. standard error. The same column of different lower case letters indicate that the difference of the densities of bemisia tabaci on tomatoes in different plots in the same planting area is obvious (P <0.05, Duncan's new double-pole difference method); a, B, C in the table represents the difference.
Table 1 shows that there is a significant difference in population density between bemisia tabaci nymphs and adults on tomatoes in different planting areas, and that the population density difference between bemisia tabaci in different plots in the same planting area is not significant; the population density of the bemisia tabaci nymphs and adults is highest in the tomato field of the riverside planting area, the population density of the tomato field of the mountain planting area is second, and the population density of the tomato field of the flower planting area is lowest; wherein the population density of the nymphs and adults in the C sample plot of the riverside planting area is 16.05 heads/leaf at most, and the population density of the nymphs and adults in the B sample plot of the flower planting area is 1.64 heads/leaf at most.
(4) Compared with the yellow board, the device of the invention has higher trapping efficiency on the Bemisia tabaci imagoes
TABLE 2 comparison of trapping efficiency of the inventive device and yellow plates against Bemisia tabaci adults
Table 2 shows that the attractant efficiency of the device of the present invention and the yellow plate to the adult bemisia tabaci is significantly different, the attraction efficiency of the yellow plate to the adult bemisia tabaci has little advantage compared with the device of the present invention before the device is set for 5min, the advantage of the attraction efficiency of the device of the present invention to the adult bemisia tabaci gradually appears after 30min, and as time goes on, the attraction efficiency of the device of the present invention becomes more obvious, and the attraction efficiency of the device of the present invention is 2.38 times that of the yellow plate attractant when the device is set to attract the adult bemisia tabaci for 5 h.
(5) Physical properties and transportation efficiency of 4 different storage and transportation devices
TABLE 34 comparison of physical Properties and transport efficiencies of different storage and transport devices
Table 3 shows that, from the viewpoint of mass, the single self-sealing bag has the lightest mass, and the single insect collecting cage has the heaviest mass; the device has the largest maximum volume in terms of volume, relatively more bemisia tabaci can be stored, although the volume of the insect collecting cage is relatively larger, the volume of the insect collecting cage is invariable, the device can be freely adjusted, the device can be compressed into 1/7-1/8 of the maximum volume even when not in use, the mass of the device is only 1/5 of the insect collecting cage, the volumes of the yellow plate and the self-sealing bag are smaller, and the number of the stored bemisia tabaci is relatively small; most importantly, the death rate of the bemisia tabaci during transportation is only 2% by using the device, the death rate of the bemisia tabaci during transportation is 10% by using the insect cage for transportation, the self-sealing bag is easily squeezed, the death rate of the bemisia tabaci during transportation is 38%, and the bemisia tabaci trapped by the yellow plate is stuck on the yellow plate, so that the death rate of the bemisia tabaci is 100%.
(6) Efficiency comparison of 4 different tobacco seedling culture methods for feeding bemisia tabaci
TABLE 44 comparison of the efficiency of different tobacco seedling culture methods for the feeding of Bemisia tabaci
Table 4 it can be seen from the results of culturing tobacco seedlings for the cultivation of bemisia tabaci by 4 different devices that the soilless culture device and the soil culture device of the present invention are the method in which the number of times of replacing tobacco seedlings and the number of times of adding water to moisturize are significantly less than those of branch water culture and air culture because living tobacco seedlings are used for the cultivation of bemisia tabaci; under the condition that other factors are the same, the growth duration of the two devices for feeding the bemisia tabaci is shorter than that of branches subjected to water culture and air culture; in addition, the egg hatchability, larva livability and emergence rate of the two breeding devices are also obviously higher than those of branch water culture and air culture during breeding; the two feeding devices greatly reduce the labor input in the using process, the feeding effect is better than that of the traditional feeding method, and the working efficiency is greatly improved.
By using the method and the structure of the invention, insects such as noctuids, trialeurodes vaporariorum, wheat moths and the like can be collected and raised by only slightly adjusting the sizes, the material methods and the like of all the parts. From the above tests and results, compared with the traditional device and method, the collecting device and the feeding device provided by the invention have the characteristics of simple operation, capability of effectively collecting and feeding various insects, wide applicable environment, high flexibility, light weight, small volume, convenience in carrying, labor force saving, low device manufacturing cost and the like, and can be used in cooperation with various insect cages, insect feeding rooms, large insect feeding and expanding sheds and the like.
The method for using the field portable collecting and feeding integrated device for the bemisia tabaci in the practical work is described in detail above. It should be noted that it is obvious to those skilled in the art that modifications or improvements, such as the adjustment of the specifications of the apparatus, can be made without departing from the structure of the present invention; therefore, unless a significant amount of innovation is introduced, all such modifications and improvements are intended to be included within the scope of the invention as claimed.
Claims (9)
1. A device for field portable collection and breeding of bemisia tabaci is characterized by comprising four parts, namely a breathable top cover (1), a compression tank (2), a breathable bottom cover (3) and a hard tank (4); the device comprises two forms formed by the four components: a) the morphological structure of the collecting device and the morphological structure of the feeding device;
the shape structure of the acquisition device comprises a ventilating top cover (1), a compression tank (2) and a ventilating bottom cover (3), wherein the ventilating top cover (1), the compression tank (2) and the ventilating bottom cover (3) are sequentially connected in a clamping manner or in a threaded manner or in a nested manner from top to bottom;
the feeding device is characterized in that the shape structure of the feeding device comprises a ventilating top cover (1), a compression tank (2) and a hard tank (4), wherein the ventilating top cover (1), the compression tank (2) and the hard tank (4) are sequentially connected in a clamping or threaded or nested manner from top to bottom;
the breathable top cover (1) and the breathable bottom cover (3) comprise annular cover bodies, breathable holes arranged in the middle of the cover bodies, top cover gauze (1.1) and bottom cover gauze (3.1) arranged in the middle of the breathable holes;
the hard tank (4) is arranged as a tank body with an opening at the top, and the tank body is made of hard material;
the device also comprises an arc-shaped movable handle (1.2), wherein two ends of the arc-shaped movable handle (1.2) are respectively fixedly arranged on the edge of the top cover (1) and movably connected with the top cover (1).
2. The device for conveniently collecting and raising the bemisia tabaci in the field as a whole as claimed in claim 1, wherein a watering opening (5) is formed in the side wall of the hard tank (4), a finger-shaped pipe (5.1) is fixedly sleeved on the watering opening (5), and a plug is arranged at the outer end of the finger-shaped pipe (5.1).
3. The field portable collecting and feeding integrated device for bemisia tabaci as claimed in claim 1, wherein the device further comprises a filter box (3.2) and a transparent sealing sheet (3.4) which are matched with the bottom cover (3) when the morphological structure of the device is adopted; the filter box (3.2) is a dish-shaped object, the transparent sealing sheet (3.4) is covered on the filter box (3.2), and the bottom of the filter box (3.2) is provided with a filter box gauze (3.3).
4. The field portable collecting and feeding integrated device for bemisia tabaci as claimed in claim 1 or 3, wherein when the device is in a feeding device shape structure, the device further comprises a conical gauze (4.1) and a hollow support sheet (4.2) which are matched with the hard tank (4); the hollow support sheet (4.2) is covered on the conical gauze (4.1), and the bottom of the conical gauze (4.1) is provided with a round base (4.3).
5. The device for collecting and raising bemisia tabaci as a whole in the field as claimed in claim 1, wherein the upper opening of the compression tank (2) is connected with the top cover (1), the lower opening of the compression tank (2) is connected with the bottom cover (3), and the lower opening of the compression tank (2) is connected with the hard tank (4) through the arranged snap fastener (6).
6. The field portable bemisia tabaci collecting and feeding integrated device as claimed in claim 4, wherein the tank body and the cover are made of transparent materials; wherein the compression tank (2) is made of transparent low-density polyethylene, the hard tank (4), the top cover (1), the bottom cover (3), the filter box (3.2), the transparent sealing sheet (3.4), the hollow supporting sheet (4.2) and the round base (4.3) are made of transparent high-density polyethylene, and the pouring gate (5) and the finger-shaped pipe (5.1) are made of transparent polypropylene; the top cover gauze (1.1), the bottom cover gauze (3.1) and the filter box gauze (3.2) are all made of nylon materials; the conical gauze (4.1) is made of stainless steel material.
7. The field portable bemisia tabaci collection and feeding integrated device as claimed in claim 4, wherein the device has the following dimensional parameters: the inner diameter of the top cover (1) is 15 cm-20 cm, the thickness is 0.2cm, and the height is 1 cm; the distance between the top cover gauze and the top cover edge is 1.5cm, and the diameter is 12 cm-17 cm; the size of the bottom cover (3) is consistent with that of the top cover (1); the diameter of a large circle of the compression tank (2) is 15 cm-20 cm, the diameter of a small circle is 12 cm-17 cm, the thickness is 0.01cm, the outer diameter of a connecting port of the compression tank (2) is 15 cm-20 cm, the height is 1cm, the thickness is 0.2cm, and the longest length of the compression tank (2) is extended to 30 cm; the thickness of the filter box (3.2) is 0.1cm, the outer diameter is equal to the inner diameter of the connecting port of the compression tank (2), and the thickness of the transparent sealing sheet (3.4) is 0.1cm, and the diameter is 15 cm-20 cm; the inner diameter of the connecting opening of the hard tank (4) is 15 cm-20 cm, the height is 1cm, the thickness is 0.2cm, the thickness of the tank body of the hard tank (4) is 0.1cm, the diameter is 15 cm-20 cm, and the height is 10 cm; the diameter of the cone-shaped gauze (4.1) is 15 cm-20 cm, the height is 10cm, the diameter of the hollow support sheet is 15 cm-20 cm, the diameter of the hollow circle is 2cm, and the thickness is 0.1 cm; the caliber of a finger-shaped pipe (5.1) matched with the pouring gate is 1.5 cm; the mesh number of the top cover gauze (1.1), the bottom cover gauze (3.1) and the filter box gauze (3.3) is 100-120 meshes, and the mesh number of the cone gauze (4.1) is 10 meshes.
8. The method for using the device for field portable collection and feeding of bemisia tabaci as claimed in claim 4, wherein the field collection comprises the following specific steps:
the method comprises the following steps: when insects are collected in an outward process, the collecting device is replaced, namely the hard tank (4) is replaced by the bottom cover (3), the filter box (3.2) and the transparent sealing sheet (3.4) are installed, and the device is compressed to 1/7-1/8 of the height of the tank body, so that the device is convenient to carry;
step two: when the device is used in a field, the bemisia tabaci sex attractant is placed in a filter box (3.2), a transparent sealing sheet (3.4) is covered to prevent the sex attractant from being diffused from a top cover (1), a bottom cover (3) is opened, a compressed tank (2) is stretched, young and tender bemisia tabaci host plants are placed in the tank, so that the attracted bemisia tabaci is attached to the host plants, and finally the device is used as an attraction device and is hung on the host plants with bemisia tabaci populations through an arc-shaped movable handle (1.2);
step three: when the attracting device is collected, the tobacco whitefly or the parasitic blades of the tobacco whitefly larvae collected manually are placed into the device, the bottom cover (3) is covered, the tobacco whitefly sex attractant and the transparent sealing sheet (3.4) are taken down, and the device is used as a device for transportation and storage.
9. The method for using the field portable collecting and feeding integrated device for the bemisia tabaci as claimed in claim 8, wherein the method comprises two methods of soil cultivation tobacco feeding and soilless cultivation tobacco feeding, and comprises the following specific steps:
the method comprises the following steps: soil cultivation of tobacco seedlings: transplanting tobacco seedlings in a seedling stage into the hard pot (4) serving as a carrier, cultivating the tobacco with soil, and feeding the bemisia tabaci when the tobacco seedlings grow to a root extending stage;
soilless culture tobacco seedling raising: putting a proper amount of nutrient solution into a hard tank (4), putting a cone-shaped gauze (4.1) into the hard tank (4), putting tobacco seedlings grown by soilless culture and a substrate into the bottom of the cone-shaped gauze (4.1), covering a hollow support sheet (4.2) to ensure that the tobacco seedlings extend out of the middle hole, and feeding bemisia tabaci when the tobacco seedlings grow to a root extending period; for the whiteflies to be raised collected in the field, the bottom cover (3) of the collecting device is detached, the compression tank (2) and the collected whiteflies are directly sleeved on the hard tank (4) with tobacco planted in advance, and the snap fastener (6) is fastened, namely living plants are used for raising insects;
step two: opening the watering port (5) to add nutrient solution to the tobacco seedlings at proper time: if the tobacco begins to wither or die, the tobacco needs to be replaced for continuous feeding, at the moment, the snap fastener (6) needs to be opened, the tobacco plant is cut, the cut tobacco plant and the compression tank are sleeved on the other hard tank (4) in which the healthy tobacco seedlings are planted, the snap fastener (6) is fastened, after the whiteflies are completely transferred from the withered tobacco plant to the healthy tobacco plant, the snap fastener (6) is opened, the withered tobacco plant is extracted, and the snap fastener (6) is fastened again, so that the replacement of the tobacco plant is completed;
step three: the growth condition of insects in the tank is observed through the transparent tank body: when the fed Bemisia tabaci needs to be taken for testing, the top cover is opened, the Bemisia tabaci is sucked by the insect suction pipe, and when the Bemisia tabaci at the lower part of the host plant needs to be sucked, the compression tank (2) is compressed until the Bemisia tabaci is exposed out of the host plant for sucking.
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