CN112425459B - Method and device for effectively collecting Morchella spores - Google Patents

Method and device for effectively collecting Morchella spores Download PDF

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Publication number
CN112425459B
CN112425459B CN202011314036.9A CN202011314036A CN112425459B CN 112425459 B CN112425459 B CN 112425459B CN 202011314036 A CN202011314036 A CN 202011314036A CN 112425459 B CN112425459 B CN 112425459B
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sealing box
drawer
module
morchella
box
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CN112425459A (en
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郭永乐
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Anhui Leyongyuan Agricultural Technology Co ltd
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Anhui Leyongyuan Agricultural Technology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/70Harvesting
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A61L2/0029Radiation
    • A61L2/0047Ultraviolet radiation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/26Accessories or devices or components used for biocidal treatment
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/20Targets to be treated
    • A61L2202/21Pharmaceuticals, e.g. medicaments, artificial body parts

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

The invention discloses a method and a device for effectively collecting Morchella spores, wherein the method comprises an auxiliary box, a base, a sealing box and a storage table, wherein the sealing box is arranged on the base; the front of the sealing box is provided with a controller, the right side of the controller is provided with a drawer, an operation opening is arranged above the drawer, operation gloves are arranged in the sealing box at the operation opening, UV lamps are further arranged on the left side and the right side of the interior of the sealing box, and a lamplight module is arranged on the rear side of the interior of the sealing box; a ventilation module is arranged above the sealing box, a choke module is arranged below the ventilation module, a hanging rope is arranged below the choke module, a flat spiral strip-shaped choke blade is arranged in the middle of the hanging rope, and a plurality of hanging hooks are arranged below the hanging rope; the rear part of the sealing box is also provided with an air outlet, a first filtering device is arranged at the air outlet of the air outlet, and the device designed by the scheme and the using method are adopted, so that the popularization of Morchella culture can be better promoted, the culture cost can be reduced, and the device has high popularization benefit.

Description

Method and device for effectively collecting Morchella spores
Technical Field
The invention relates to the technical field of edible fungus culture, in particular to the technical field of edible fungus spore collection, and particularly relates to a method and a device for effectively collecting Morchella spores.
Background
Morchella has crisp and tender taste and delicious taste, has the medicinal effects of regulating immunity, is a world famous edible and medicinal fungus, is a traditional food in European and American countries, and has limited wild resources and very high price. Therefore, the artificial domestication cultivation of the morchella is successfully realized, and great economic and ecological benefits are achieved. The artificial domestication cultivation technology of Morchella has a research history of nearly century at home and abroad, is a world difficult problem, and on the basis of repeated experiments and experience accumulation of several generations of scientists, successful artificial cultivation of Morchella has been reported in recent years, but unfortunately, large-area cultivation popularization and commercial production of Morchella are not seen so far, and the following reasons are included: firstly, morchella mycelium is easy to fail in the process of converting nutrition growth into reproduction growth, namely the fruiting difficulty of Morchella under artificial conditions is high. And secondly, the input cost is higher, and the yield is relatively lower. In addition, the possibility of mixing mixed bacteria is very high in the process of collecting spores of Morchella esculenta, and the development and popularization of Morchella esculenta cultivation are restricted all the time.
Disclosure of Invention
The invention aims to solve the problems, through improving the Morchella spore collection process and designing Morchella spore collection equipment, the occurrence of mixed bacteria can be effectively reduced, meanwhile, the spore collection efficiency can be improved, the mixed bacteria and bacteria can be effectively killed through the arranged ventilation module, the purity in the sealed box can be effectively ensured through the arranged UV lamp and the electric heating silk screen, and the hanging rope can be driven to slowly rotate under the breeze condition through the spiral strip-shaped wind blocking blades arranged on the designed hanging rope, so that the light irradiation is more uniform and the spore ejection efficiency is high; the wind blocking module can prevent the problem that the spores are blown away by wind from blowing directly; through the positive pressure condition in the designed sealed box, sundry bacteria can be effectively prevented from entering the sealed box during exchange operation, and pollution is prevented; by adopting the device and the using method, the popularization of Morchella culture can be better promoted, the culture cost can be reduced, and the device and the using method have high popularization benefits.
To achieve the above object, the present invention is achieved by:
a method and a device for effectively collecting Morchella spores comprise an auxiliary box, wherein the auxiliary box comprises a base, a sealing box is arranged on the base, and a storage table is arranged on the right side of the sealing box; the front of the sealing box is provided with a controller, the right side of the controller is provided with a drawer, an operation opening is arranged above the drawer, operation gloves are arranged in the sealing box at the operation opening, UV lamps are further arranged on the left side and the right side of the interior of the sealing box, and a lamplight module is arranged on the rear side of the interior of the sealing box; a ventilation module is arranged above the sealing box, a choke module is arranged below the ventilation module, a hanging rope is arranged below the choke module, a flat spiral strip choke blade is arranged in the middle of the hanging rope, and a plurality of hanging hooks are arranged below the hanging rope; the rear part of the sealing box is also provided with an air outlet, and a first filtering device is arranged at the air outlet of the air outlet.
As a better technical scheme, the upper portion of seal box is transparent material and makes, and the upper portion of seal box still is provided with shading cloth.
As a better technical scheme, temperature sensor, humidity transducer, wind speed sensor are still installed to the inside of seal box, and temperature sensor, humidity transducer, wind speed sensor distribute in the corner of seal box.
As a better technical scheme, one end of the drawer is positioned at the lower center of the front surface of the sealing box, a sealing strip is arranged at the position, which is contacted with the sealing box, of the drawer, a shell is arranged at the position, which is positioned in the sealing box, of the drawer, the upper part of the shell is a turnover upper cover, the turnover upper cover is made of transparent materials, and a sealing rubber strip is arranged at the contact part of the turnover upper cover and the shell; the rotary shaft of the reversible upper cover is arranged at the front inner position of the sealing box, and is in a sealing state when the reversible upper cover is contacted with the shell.
As a better technical scheme, the ventilation module comprises a ventilation pipe, and a second filtering device is arranged at the upper part of the ventilation pipe; a ventilation pump is arranged below the second filtering device; a sterilizing device, a cooling device and a humidity control device are sequentially arranged below the ventilation pump.
As a better technical scheme, the second filter device is a ceramic filling active carbon filter device, the sterilization device is an electric heating wire mesh, the cooling device comprises a cooling water pipe network, and the humidity control device comprises a spraying device.
As a better technical scheme, the sterilization device comprises a plurality of electric heating wire meshes, wherein the electric heating wire meshes are arranged in a vertically laminated way, and gaps are reserved between the electric heating wire meshes; the cooling water pipe network is also connected with a water cooling module; purified water is sprayed out of the spraying device, and an anti-condensation device is further arranged below the spraying device.
As a better technical scheme, the spraying device is an ultrasonic mist generating device, a water collector is arranged below a nozzle of the spraying device, and a water outlet of the water collector is connected with a wastewater tank through a pipeline.
As a better technical scheme, the choke module is of a conical structure, and the axis of the choke module and the axis of the ventilation pipe are located on the same axis.
As a better technical scheme, the invention also comprises a spore collection method, which comprises the following steps:
A. using a controller, turning on a power supply, turning on a UV lamp, and performing sterilization operation;
B. after sterilization, closing a UV lamp, opening a light module, opening an electric heating silk screen power supply, then opening a ventilation pump, controlling the temperature of a sealing box to be 70-80 ℃, keeping for a period of time, sequentially opening a cooling device and a humidity control device, adjusting the temperature in the sealing box to be 20+/-1 ℃ and the humidity to be 50-60%, measuring the wind speed to be 4-4.5 m/s, and opening a drawer, wherein at the moment, the drawer is opened without mixed bacteria because the sealing box is in positive pressure;
C. placing active Morchella to be collected into a drawer, placing a culture dish into the drawer, closing the drawer, and simultaneously adjusting a controller to ensure that the measuring wind speed is kept between 0.1m/s and 0.15m/s, and the temperature in a sealed box is kept at 20+/-1 ℃ and the humidity is kept at 50% -60%;
D. the user inserts the hands into the operation glove to start operation;
E. opening the reversible upper cover, taking out the active Morchella and the culture dish respectively, placing the culture dish at a proper position, closing the reversible upper cover, starting to divide the active Morchella, hanging the divided active Morchella on a hanging hook, and adjusting the position of the culture dish to enable spores to fall into the culture dish after ejection;
F. adjusting a light module to ensure that the light is white light and the power is 50-80W, keeping the temperature and the humidity, putting down shading cloth, and keeping for 2 hours;
G. taking down the shading cloth, and inserting the two hands into the operation glove by a user to start operation;
H. covering the culture dish with a cover, opening the turnover upper cover, placing the culture dish into a drawer, adjusting the measuring wind speed to be kept at 4-4.5 m/s, opening the drawer, and taking out the culture dish;
I. the morchella spores can be transferred and re-cultured;
J. after the use is completed, the humidity control device is closed, the cooling device is closed, the measured wind speed is kept at 2-2.5 m/s, the UV lamp is turned on, the temperature of the sealing box is controlled to be 70-80 ℃ for a period of time, then the ventilation pump and the electric heating silk screen are closed, the UV lamp and the light module are closed, and the UV lamp and the light module are placed for standby.
The beneficial effects of the scheme are that: through improving the process of Morchella spore collection and designing the equipment for Morchella spore collection, the occurrence of miscellaneous bacteria can be effectively reduced, meanwhile, the efficiency of spore collection can be improved, through the ventilation module, the miscellaneous bacteria and bacteria can be effectively killed, the purity in the sealed box can be effectively ensured through the arranged UV lamp and the electric heating silk screen, the spiral strip-shaped fan blocking blade arranged on the designed hanging rope can drive the hanging rope to slowly rotate under the breeze condition, so that the light irradiation is more uniform, and the spore ejection efficiency is high; the wind blocking module can prevent the problem that the spores are blown away by wind from blowing directly; through the positive pressure condition in the designed sealed box, sundry bacteria can be effectively prevented from entering the sealed box during exchange operation, and pollution is prevented; by adopting the device and the using method, the popularization of Morchella culture can be better promoted, the culture cost can be reduced, and the device and the using method have high popularization benefits.
None of the parts of the device are the same as or can be implemented using prior art.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention. In the drawings:
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram showing a combination structure of a hanging rope and a flat spiral strip-shaped wind-blocking blade;
FIG. 3 is a schematic view of a choke module according to the present invention;
FIG. 4 is a partial perspective view of another structural schematic of the choke module of the present invention; compared with fig. 3, the structure is provided with a plurality of through holes, and the through holes are not through holes, so that the wind guiding performance can be effectively reduced, and the wind is disordered when the wind blows down;
FIG. 5 is a schematic top view of the sterilization apparatus of the present invention;
FIG. 6 is a schematic diagram of a front view of a ventilation module according to the present invention;
in the figure: 1. a base; 2. a seal box; 3. a storage table; 4. a controller; 5. a drawer; 6. an operation port; 7. an operating glove; uv lamp; 9. a light module; 10. a ventilation module; 1001. a second filtering device; 1002. a ventilation pump; 1003. a sterilization device; 1004. a cooling device; 1005. a humidity control device; 11. a choke module; 12. a hanging rope; 13. spiral strip-shaped wind-blocking blades; 14. and an air outlet.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
1-6, a method and a device for effectively collecting Morchella spores comprise an auxiliary box, wherein the auxiliary box comprises a base, a sealing box is arranged on the base, and a storage table is arranged on the right side of the sealing box; the front of the sealing box is provided with a controller, the right side of the controller is provided with a drawer, an operation opening is arranged above the drawer, operation gloves are arranged in the sealing box at the operation opening, UV lamps are further arranged on the left side and the right side of the interior of the sealing box, and a lamplight module is arranged on the rear side of the interior of the sealing box; a ventilation module is arranged above the sealing box, a choke module is arranged below the ventilation module, a hanging rope is arranged below the choke module, a flat spiral strip choke blade is arranged in the middle of the hanging rope, and a plurality of hanging hooks are arranged below the hanging rope; the rear part of the sealing box is also provided with an air outlet, and a first filtering device is arranged at the air outlet of the air outlet.
As a better technical scheme, the upper portion of seal box is transparent material and makes, and the upper portion of seal box still is provided with shading cloth.
As a better technical scheme, temperature sensor, humidity transducer, wind speed sensor are still installed to the inside of seal box, and temperature sensor, humidity transducer, wind speed sensor distribute in the corner of seal box.
As a better technical scheme, one end of the drawer is positioned at the lower center of the front surface of the sealing box, a sealing strip is arranged at the position, which is contacted with the sealing box, of the drawer, a shell is arranged at the position, which is positioned in the sealing box, of the drawer, the upper part of the shell is a turnover upper cover, the turnover upper cover is made of transparent materials, and a sealing rubber strip is arranged at the contact part of the turnover upper cover and the shell; the rotary shaft of the reversible upper cover is arranged at the front inner position of the sealing box, and is in a sealing state when the reversible upper cover is contacted with the shell.
As a better technical scheme, the ventilation module comprises a ventilation pipe, and a second filtering device is arranged at the upper part of the ventilation pipe; a ventilation pump is arranged below the second filtering device; a sterilizing device, a cooling device and a humidity control device are sequentially arranged below the ventilation pump.
As a better technical scheme, the second filter device is a ceramic filling active carbon filter device, the sterilization device is an electric heating wire mesh, the cooling device comprises a cooling water pipe network, and the humidity control device comprises a spraying device.
As a better technical scheme, the sterilization device comprises a plurality of electric heating wire meshes, wherein the electric heating wire meshes are arranged in a vertically laminated way, and gaps are reserved between the electric heating wire meshes; the cooling water pipe network is also connected with a water cooling module; purified water is sprayed out of the spraying device, and an anti-condensation device is further arranged below the spraying device.
As a better technical scheme, the spraying device is an ultrasonic mist generating device, a water collector is arranged below a nozzle of the spraying device, and a water outlet of the water collector is connected with a wastewater tank through a pipeline.
As a better technical scheme, the choke module is of a conical structure, and the axis of the choke module and the axis of the ventilation pipe are located on the same axis.
Example two
As shown in FIG. 3, the choke module adopts a solid cone structure, the wind speed is measured at each corner relatively evenly, the wind flow at the position where the culture dishes are placed is slightly disordered, then a test is performed, 8 culture dishes are placed and are circumferentially arranged at the correct placement position of the culture dishes, and after spores are found to be ejected, part of spores fall outside the correct placement position of the culture dishes, and the proportion is about 3-5%.
Example III
As shown in FIG. 4, the choke module adopts a conical structure with a plurality of through holes, when the wind speed is measured, the wind speed at each corner is smaller, the culture dish part also has breeze, at the moment, when spores are ejected, the wind direction is approximately downward, the test is carried out again, 8 culture dishes are placed, the culture dishes are circumferentially placed at the correct placement positions of the culture dishes, and after the spores are ejected, part of the spores fall outside the correct placement positions of the culture dishes, and the proportion is approximately 1-1.5%. However, because of the high processing cost, the choke module shown in fig. 3 is still selected, and the economic return rate is higher.
As a better technical scheme, the invention also comprises a spore collection method, which comprises the following steps:
A. using a controller, turning on a power supply, turning on a UV lamp, and performing sterilization operation;
B. after sterilization, closing a UV lamp, opening a light module, opening an electric heating silk screen power supply, then opening a ventilation pump, controlling the temperature of a sealing box to be 70-80 ℃, keeping for a period of time, sequentially opening a cooling device and a humidity control device, adjusting the temperature in the sealing box to be 20+/-1 ℃ and the humidity to be 50-60%, measuring the wind speed to be 4-4.5 m/s, and opening a drawer, wherein at the moment, the drawer is opened without mixed bacteria because the sealing box is in positive pressure;
C. placing active Morchella to be collected into a drawer, placing a culture dish into the drawer, closing the drawer, and simultaneously adjusting a controller to ensure that the measuring wind speed is kept between 0.1m/s and 0.15m/s, and the temperature in a sealed box is kept at 20+/-1 ℃ and the humidity is kept at 50% -60%;
D. the user inserts the hands into the operation glove to start operation;
E. opening the reversible upper cover, taking out the active Morchella and the culture dish respectively, placing the culture dish at a proper position, closing the reversible upper cover, starting to divide the active Morchella, hanging the divided active Morchella on a hanging hook, and adjusting the position of the culture dish to enable spores to fall into the culture dish after ejection;
F. adjusting a light module to ensure that the light is white light and the power is 50-80W, keeping the temperature and the humidity, putting down shading cloth, and keeping for 2 hours;
G. taking down the shading cloth, and inserting the two hands into the operation glove by a user to start operation;
H. covering the culture dish with a cover, opening the turnover upper cover, placing the culture dish into a drawer, adjusting the measuring wind speed to be kept at 4-4.5 m/s, opening the drawer, and taking out the culture dish;
I. the morchella spores can be transferred and re-cultured;
J. after the use is completed, the humidity control device is closed, the cooling device is closed, the measured wind speed is kept at 2-2.5 m/s, the UV lamp is turned on, the temperature of the sealing box is controlled to be 70-80 ℃ for a period of time, then the ventilation pump and the electric heating silk screen are closed, the UV lamp and the light module are closed, and the UV lamp and the light module are placed for standby.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (3)

1. Device of effective collection morchella spore, its characterized in that: the automatic storage box comprises an auxiliary box, wherein the auxiliary box comprises a base, a sealing box is arranged on the base, and a storage table is further arranged on the right side of the sealing box; the front of the sealing box is provided with a controller, the right side of the controller is provided with a drawer, an operation opening is arranged above the drawer, operation gloves are arranged in the sealing box at the operation opening, UV lamps are further arranged on the left side and the right side of the interior of the sealing box, and a lamplight module is arranged on the rear side of the interior of the sealing box; a ventilation module is arranged above the sealing box, a choke module is arranged below the ventilation module, a hanging rope is arranged below the choke module, a flat spiral strip choke blade is arranged in the middle of the hanging rope, and a plurality of hanging hooks are arranged below the hanging rope; an air outlet is further formed in the rear portion of the sealing box, and a first filtering device is arranged at an air outlet of the air outlet;
the ventilation module comprises a ventilation pipe, and a second filtering device is arranged at the upper part of the ventilation pipe; a ventilation pump is arranged below the second filtering device; a sterilizing device, a cooling device and a humidity control device are sequentially arranged below the ventilation pump;
the second filter device is a ceramic filling active carbon filter device, the sterilization device is an electric heating wire mesh, the cooling device comprises a cooling water pipe network, and the humidity control device comprises a spraying device;
the sterilizing device comprises a plurality of electric heating wire meshes, wherein the electric heating wire meshes are arranged in a stacked manner up and down, and gaps are reserved between the electric heating wire meshes; the cooling water pipe network is also connected with a water cooling module; purified water is sprayed out of the spraying device;
the upper part of the sealing box is made of transparent materials, and the upper part of the sealing box is also provided with shading cloth;
a temperature sensor, a humidity sensor and a wind speed sensor are also arranged in the sealing box, and the temperature sensor, the humidity sensor and the wind speed sensor are distributed at the corners of the sealing box;
one end of the drawer is positioned at the lower center of the front surface of the sealing box, a sealing strip is arranged at the position, which is contacted with the sealing box, of the drawer, a shell is arranged at the position, which is positioned inside the sealing box, of the drawer, the upper part of the shell is a turnover upper cover which is made of transparent materials, and a sealing rubber strip is arranged at the contact part of the turnover upper cover and the shell; the rotary shaft of the reversible upper cover is arranged at the inner position of the front surface of the sealing box, and is in a sealing state when the reversible upper cover is contacted with the shell;
the choke module is of a conical structure, and the axis of the choke module and the axis of the ventilation pipe are located on the same axis.
2. A device for efficiently collecting morchella spores according to claim 1, wherein: the spraying device is an ultrasonic mist generating device, a water collector is arranged below a nozzle of the spraying device, and a water outlet of the water collector is connected with a wastewater tank through a pipeline.
3. A method of using a device for efficiently collecting morchella spores according to claim 2, wherein: the spore collection method comprises the following steps:
using a controller, turning on a power supply, turning on a UV lamp, and performing sterilization operation;
after sterilization, closing a UV lamp, opening a light module, opening an electric heating silk screen power supply, then opening a ventilation pump, controlling the temperature of a sealing box to be 70-80 ℃, keeping for a period of time, sequentially opening a cooling device and a humidity control device, adjusting the temperature in the sealing box to be 20+/-1 ℃ and the humidity to be 50-60%, measuring the wind speed to be 4-4.5 m/s, and opening a drawer, wherein at the moment, the drawer is opened without mixed bacteria because the sealing box is in positive pressure;
placing active Morchella to be collected into a drawer, placing a culture dish into the drawer, closing the drawer, and simultaneously adjusting a controller to ensure that the measuring wind speed is kept between 0.1m/s and 0.15m/s, and the temperature in a sealed box is kept at 20+/-1 ℃ and the humidity is kept at 50% -60%;
the user inserts the hands into the operation glove to start operation;
opening the reversible upper cover, taking out the active Morchella and the culture dish respectively, placing the culture dish at a proper position, closing the reversible upper cover, starting to divide the active Morchella, hanging the divided active Morchella on a hanging hook, and adjusting the position of the culture dish to enable spores to fall into the culture dish after ejection;
adjusting a light module to ensure that the light is white light and the power is 50-80W, keeping the temperature and the humidity, putting down shading cloth, and keeping for 2 hours;
taking down the shading cloth, and inserting the two hands into the operation glove by a user to start operation;
covering the culture dish with a cover, opening the turnover upper cover, placing the culture dish into a drawer, adjusting the measuring wind speed to be kept at 4-4.5 m/s, opening the drawer, and taking out the culture dish;
the morchella spores can be transferred and re-cultured;
after the use is completed, the humidity control device is closed, the cooling device is closed, the measured wind speed is kept at 2-2.5 m/s, the UV lamp is turned on, the temperature of the sealing box is controlled to be 70-80 ℃ for a period of time, then the ventilation pump and the electric heating silk screen are closed, the UV lamp and the light module are closed, and the UV lamp and the light module are placed for standby.
CN202011314036.9A 2020-11-20 2020-11-20 Method and device for effectively collecting Morchella spores Active CN112425459B (en)

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