CN209010517U - A kind of new and effective spore acquisition device - Google Patents
A kind of new and effective spore acquisition device Download PDFInfo
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- CN209010517U CN209010517U CN201820944381.2U CN201820944381U CN209010517U CN 209010517 U CN209010517 U CN 209010517U CN 201820944381 U CN201820944381 U CN 201820944381U CN 209010517 U CN209010517 U CN 209010517U
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Abstract
The utility model discloses a kind of new and effective spore acquisition devices, including pallet, culture dish, liner, kind mushroom support, shield and outer casing, the shield is made of frame and interior wrapping layer, the utility model shield is instead of the glass bell jar in traditional spore acquisition device, environmental condition needed for significantly improving kind of mushroom ejection spore, the ejection for being conducive to basidiospore, realize separated, breeding individual it is pure；With kind of mushroom support instead of the kind mushroom bracket of the prior art, separatory kind of mushroom can be enable to be placed in culture dish bottom more preferable, more stablely in this way, be unlikely to dilapidated and make kind of a massee fruiting bodies block touch ware bottom bottom or its along upper and pollute, to also reduce a possibility that it pollutes launched basidiospore；Outer casing is set, realize the target for effectively extraneous miscellaneous bacteria being stopped to be infected and both saved drug more protect the safety of operator.
Description
Technical field
The utility model relates to a kind of acquisition device, specifically a kind of new and effective spore acquisition device.
Background technique
Spore acquisition device is also referred to as spore collector, it is can for acquire the sexual basidiospore of Agaricales gill fungus bacterium
It leans on, effective and the most commonly used device, it can achieve the purpose that aseptic collection spore well.Traditional spore acquisition device point
Be two kinds: one is by pallet, two layers of pure cotton spun yarn layer of cloth pad, culture dish it is a set of (ware lid oral area is buckled in downwards on gauze layer pad,
The downward mouth in ware bottom bottom is upward to be placed in ware lid), kind mushroom bracket, the composition such as glass bell jar (on oral area tampon)；Another kind is
It is made of triangular flask (the built-in CPDA plating medium of bottle), the stainless steel steel wire hook of hook kind mushroom, tampon etc., traditional spore is adopted
Acquisition means have the disadvantage in that in (1) glass bell jar or triangular flask that humidity is big, aqueous vapor weight, often grow the kind mushroom for launching spore
Dense aerial hyphae, spue many droplets；And have no the ejection of spore；(2) with bracket support, kind mushroom mushroom block is unstable easily inclines
, thus it can touch the bottom at ware bottom and along above causing the contaminated phenomenon of separation operation process to occur；(3) with this two kinds
Device does not acquire basidiospore often；(4) a large amount of germ plasm resources, manpower and time, efficiency and reliability pole bottom are wasted；(5) it passes
There is grave error or defects in operating procedure of uniting.
Utility model content
The purpose of this utility model is to provide a kind of new and effective spore acquisition devices, to solve in above-mentioned background technique
The problem of proposition.
To achieve the above object, the utility model provides the following technical solutions:
A kind of new and effective spore acquisition device, including pallet and culture dish, the culture dish include culture ware lid and training
Ware bottom is supported, liner is equipped in the pallet, culture ware lid is placed on liner, cultivates the lid mouth down of ware lid, cultivates ware lid
Top is placed with culture dish bottom, culture dish bottom it is opening up, kind of a mushroom support, described kind of mushroom branch are placed in the culture dish bottom
Seat is formed by an one metal wire coiling, and described kind of mushroom support includes that collet, interconnecting piece and support are combed, and collet is annular, and support is combed
It is that circle is netted, support is combed above collet, is supported and is placed with kind of a mushroom block on combing, and is led between support comb and collet on support comb
Interconnecting piece connection is crossed, is located at outside culture dish on the pallet and buckles equipped with shield, the shield is by frame and interior wrapping layer
It constitutes, the interior wrapping layer is coated in frame roof and side wall, and outer casing, outsourcing are equipped with outside the pallet and shield
There is tying band in opening system at the top of covering layer.
As a further solution of the present invention: the specification of the culture dish is 150mm.
As a further solution of the present invention: trying to get to the heart of a matter as round and diameter for the pallet 8 is 260mm, the lining
Pad 9 is made of two layers of pure cotton crocus cloth and it is having a size of 30cm square.
As a further solution of the present invention: the material of the pallet is one of aluminium and stainless steel.
As a further solution of the present invention: the material of the wire is one of aluminium and stainless steel.
As a further solution of the present invention: the frame is stainless steel in tubbiness and material, frame is by the first circle
Ring, support rod and the second annulus are constituted, and the first annulus is located at right above the second annulus, are passed through between the first annulus and the second annulus
The more support rods connection that circumferential direction is placed equidistant with.
As a further solution of the present invention: the distance between first annulus and second annulus are 210mm, the
The diameter of one annulus is 200mm, and the diameter of the second annulus is 210mm, the company of the support rod and the first annulus and the second annulus
Mode is connect preferably to weld.
As a further solution of the present invention: the outer casing and interior wrapping layer are fabric by two layers of pure cotton spun yarn
At.
As a further solution of the present invention: the tying band is lace cord.
Compared with prior art, the utility model has the beneficial effects that the utility model shield is instead of traditional spore
Glass bell jar in sub- acquisition device.Environmental condition needed for this measure significantly improves kind of mushroom ejection spore；Its pole has
Conducive to the ejection of basidiospore, and be unfavorable for kind of a mushroom lid germination and growth aerial hyphae, be also unfavorable for bacterium, saccharomycete sprouting and
Collected basidiospore is infected, realize separated, breeding individual it is pure；The utility model with kind of mushroom support instead of
The kind mushroom bracket of the prior art, can enable separatory kind of mushroom be placed in culture dish bottom more preferable, more stablely in this way, be unlikely to
It is dilapidated and make kind of a massee fruiting bodies block touch ware bottom bottom or its along upper and pollute, thus also reduce its to bullet
A possibility that penetrating basidiospore pollution；Outer casing is arranged, the target for effectively stopping extraneous miscellaneous bacteria to be infected is realized.Existing skill
Art be protected by pouring into the mercuric chloride medical fluid of 0.25% bromogeramine or 0.1% on the gauze to glass bell jar oral area surrounding or
Stop the intrusion of extraneous miscellaneous bacteria；And the utility model is, protects miscellaneous bacteria with the outer casing that two layers of pure cotton crocus cloth are made into
's.In this way, both saved drug more protect the safety of operator.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of new and effective spore acquisition device.
Fig. 2 is the structural schematic diagram of frame in new and effective spore acquisition device.
Fig. 3 is the structural schematic diagram that mushroom support is planted in new and effective spore acquisition device.
Fig. 4 is the top view of Fig. 3.
In figure: 1- lace cord, 2- outer casing, 3- shield, 4- kind mushroom block, 5- kind mushroom support, 6- culture dish bottom, 7- training
Support ware lid, 8- pallet, 9- liner, 10- frame, the first annulus of 11-, 12- support rod, the second annulus of 13-, 14- interconnecting piece, 15-
Collet, 16- support are combed.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Please refer to Fig. 1~4, in the utility model embodiment, a kind of new and effective spore acquisition device, including 8 He of pallet
Culture dish, the culture dish include culture ware lid 7 and culture dish bottom 6, and the specification of culture dish is 150mm, are laid in the pallet 8
There is liner 9, trying to get to the heart of a matter as round and diameter for pallet 8 is 260mm, and the liner 9 is made of two layers of pure cotton crocus cloth and its size
For 30cm square, the material of pallet 8 is one of aluminium and stainless steel, and culture ware lid 7 is placed on liner 9, culture ware lid 7
Lid mouth down is cultivated and is placed with culture dish bottom 6 at the top of ware lid 7, opening up, the placement in the culture dish bottom 6 at culture dish bottom 6
There is kind of a mushroom support 5, described kind of mushroom support 5 is formed by an one metal wire coiling, and the material of the wire is in aluminium and stainless steel
One kind, described kind of mushroom support 5 includes that collet 15, interconnecting piece 14 and support comb 16, and collet 15 is annular, and support combs 16 as circle
It is netted, it supports and combs 16 positioned at 15 top of collet, support and be placed with kind of a mushroom block 4 on comb 16, support comb 16 and collet on support comb 16
It is connected between 15 by interconnecting piece 14, is located at outside culture dish on the pallet 8 and buckle equipped with shield 3, the shield 3 is by frame
Frame 10 and interior wrapping layer are constituted, and the frame 10 is stainless steel in tubbiness and material, and frame 10 is by the first annulus 11, support rod 12
It is constituted with the second annulus 13, the first annulus 11 is located at right above the second annulus 13, between the first annulus 11 and the second annulus 13
Distance is 210mm, and the diameter of the first annulus 11 is 200mm, and the diameter of the second annulus 13 is 210mm, the first annulus 11 and second
Pass through circumferential more support rods 12 connection being placed equidistant with, support rod 12 and the first annulus 11 and the second annulus 13 between annulus 13
Connection type preferably weld, the interior wrapping layer is coated on the top of frame 10 and side wall, the pallet 8 and shield 3
Outside is equipped with outer casing 2, and there is tying band 1 in the opening system at 2 top of outer casing, and the tying band 1 is lace cord, can be passed through
The opening at 2 top of outer casing is carried out the survivor of a murder attempt and tightened by tying band 1, and the outer casing 2 and interior wrapping layer are by two layers of pure cotton
Crocus cloth is made.
The assemble method of the new and effective spore acquisition device are as follows: take a pallet 8, taking two layers of about 30cm square
Thin cotton gauze pads 9 place mats inside pallet；Then, 7 mouthfuls of the culture ware lid of culture dish are detained downwards and is placed on liner 9, made
6 mouthfuls of culture dish bottom is upwards, bottom is downward is placed in culture ware lid 7；Kind of mushroom support 5 is put within culture dish bottom 6, by shield
The formation that 3 oral areas are downward, closed end is upward is buckled on pallet 8；Finally, outer casing 2 from 8 bottom of pallet to coming up whole
Covering device is packed into it, and is laid flat, is put and just tighten outer casing oral area afterwards, and with lace cord, 1 survivor of a murder attempt is tightened, in this way, a set of agaric
The sexual basidiospore collector of mesh gill fungus bacterium, which just combines, to be completed.
The working principle of the utility model is: the utility model is as follows in application method:
It is described by taking the acquisition of oyster mushroom basidiospore as an example:
1) take the utility model spore acquisition device a set of, with 0.125Mpa high pressure sterilization four in vertical high-pressure sterilizing pot
Ten minutes, taking-up was dried for standby in electrically heated drying cabinet.
2) the oyster mushroom kind of breeding to be separated is chosen, and cultivates or selects kind of a massee fruiting bodies, that is, is chosen in screening range
Nine to very maturity kind mushroom as separation object.The kind mushroom sample of picking is put into paper bag and takes back transfer room (desinfection chamber)
It is spare.
3) by the utility model spore acquisition device for having sterilized and having dried, the kind mushroom sample of acquisition, 75% ethyl alcohol, cotton swab,
The utensils such as alcolhol burner, operating scissors, surgical forceps, knife blade, marking pen, label paper, shallow bid and drug are put into sterile board together,
Closed smog suffocating sterilization is carried out with aerial fog disinfectant by every cubic metre of 12 grams of dosage to handle 1 to one point five hours.
4) separation personnel are with muzzling, cleaned with suds and dry both hands.Both hands are protruded into sterile board, first with 75%
Ethyl alcohol is thoughtful, the both hands that meticulously wipe oneself are to carry out disinfection to it, then the kind mushroom sample in paper bag is taken out, is used
75% ethyl alcohol cotton swab carries out comprehensively careful cleaning disinfection two times to kind of massee fruiting bodies cap, and the kind mushroom after disinfection is put into through wiping
It wipes in the stainless steel shallow bid of disinfection.Then to knife blade, tweezers carry out flame calcination sterilizing and natural cooling it.
5) the 2 oral area lace cord 1 of outer casing for unlocking acquisition device, takes off outer casing 2, raises shield 3.It is fast later
From the cap outer, the fritter of about 3cm square is cut with scalpel from kind of massee fruiting bodies for speed, make lamella downwards, cap to
On be placed on kind of mushroom support, cover shield 3, wrap outer casing 2 and tie mouth.Acquisition device is taken out from sterile board,
Set 16 ~ 28 DEG C, in the gnotobasis of 70% relative air humidity, culture can smoothly obtain a large amount of, high-quality for about five to ten hours
Oyster mushroom basidiospore.
If in large quantities it was verified that constructing spore acquisition device in strict accordance with the embodiment of the utility model, in strict accordance with
The utility model basidiospore acquisition technique specification is operated, then can absolutely collect a large amount of, good load spore
Son！And acquisition time is only used five to ten hours.It is very reliable, quick and convenient.
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and
And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this is practical new in other specific forms
Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this is practical new
The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing for the equivalent requirements of the claims will be fallen in
All changes in justice and range are embraced therein.It should not treat any reference in the claims as limiting
Related claim.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (9)
1. a kind of new and effective spore acquisition device, including pallet (8) and culture dish, the culture dish includes culture ware lid (7)
With culture dish bottom (6), the pallet (8) is interior to be equipped with liner (9), and culture ware lid (7) is placed on liner (9), cultivates ware lid
(7) lid mouth down, culture ware lid (7) top are placed with culture dish bottom (6), opening up, the culture of culture dish bottom (6)
Kind of a mushroom support (5) is placed in ware bottom (6), which is characterized in that described kind of mushroom support (5) is formed by an one metal wire coiling, institute
It states kind of a mushroom support (5) to include collet (15), interconnecting piece (14) and support comb (16), collet (15) is annular, and support comb (16) is
Circle is netted, and support comb (16) is located above collet (15), supports and is placed with kind of a mushroom block (4) on comb (16), on support comb (16)
It is connect between support comb (16) and collet (15) by interconnecting piece (14), buckles and be equipped with outside culture dish on the pallet (8)
Shield (3), the shield (3) are made of frame (10) and interior wrapping layer, and the interior wrapping layer is coated on frame (10) top
In portion and side wall, outer casing (2) are equipped with outside the pallet (8) and shield (3), the opening at the top of outer casing (2)
System has tying band (1).
2. new and effective spore acquisition device according to claim 1, which is characterized in that the specification of the culture dish is
150mm。
3. new and effective spore acquisition device according to claim 1, which is characterized in that the pallet (8) try to get to the heart of a matter for
Round and diameter is 260mm, and the liner (9) is made of two layers of pure cotton crocus cloth and it is having a size of 30cm square.
4. new and effective spore acquisition device according to claim 1, which is characterized in that the material of the pallet (8) is
One of aluminium and stainless steel.
5. new and effective spore acquisition device according to claim 1, which is characterized in that the material of the wire is aluminium
One of with stainless steel.
6. new and effective spore acquisition device according to claim 1, which is characterized in that the frame (10) in tubbiness and
Material is stainless steel, and frame (10) is made of the first annulus (11), support rod (12) and the second annulus (13), the first annulus (11)
Right above the second annulus (13), pass through circumferential more branch being placed equidistant between the first annulus (11) and the second annulus (13)
Strut (12) connection.
7. new and effective spore acquisition device according to claim 6, which is characterized in that first annulus (11) and the
The distance between two annulus (13) are 210mm, and the diameter of the first annulus (11) is 200mm, and the diameter of the second annulus (13) is
210mm, the support rod (12) are welding with the connection type of the first annulus (11) and the second annulus (13).
8. new and effective spore acquisition device according to claim 1, which is characterized in that the outer casing (2) and interior
Wrapping layer is made of two layers of pure cotton crocus cloth.
9. new and effective spore acquisition device according to claim 1, which is characterized in that the tying band (1) is cotton thread
Rope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820944381.2U CN209010517U (en) | 2018-06-19 | 2018-06-19 | A kind of new and effective spore acquisition device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820944381.2U CN209010517U (en) | 2018-06-19 | 2018-06-19 | A kind of new and effective spore acquisition device |
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| Publication Number | Publication Date |
|---|---|
| CN209010517U true CN209010517U (en) | 2019-06-21 |
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ID=66828484
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| Application Number | Title | Priority Date | Filing Date |
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| CN201820944381.2U Active CN209010517U (en) | 2018-06-19 | 2018-06-19 | A kind of new and effective spore acquisition device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112425459A (en) * | 2020-11-20 | 2021-03-02 | 安徽乐永园农业科技有限公司 | Method and device for effectively collecting morchella spores |
-
2018
- 2018-06-19 CN CN201820944381.2U patent/CN209010517U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112425459A (en) * | 2020-11-20 | 2021-03-02 | 安徽乐永园农业科技有限公司 | Method and device for effectively collecting morchella spores |
| CN112425459B (en) * | 2020-11-20 | 2023-10-24 | 安徽乐永园农业科技有限公司 | Method and device for effectively collecting Morchella spores |
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