CN112940936A - Portable aseptic cell culture case has - Google Patents
Portable aseptic cell culture case has Download PDFInfo
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- CN112940936A CN112940936A CN202110234705.XA CN202110234705A CN112940936A CN 112940936 A CN112940936 A CN 112940936A CN 202110234705 A CN202110234705 A CN 202110234705A CN 112940936 A CN112940936 A CN 112940936A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 20
- 238000009434 installation Methods 0.000 claims description 13
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- 230000006978 adaptation Effects 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000005070 sampling Methods 0.000 abstract description 7
- 239000000428 dust Substances 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 36
- 230000000694 effects Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The invention relates to the technical field of cell culture boxes, in particular to a portable sterile cell culture box which comprises a body, wherein two first cross rods which are symmetrically arranged are rotatably connected between the inner walls of the left side and the right side of the body; compared with the prior art, the invention is convenient for sampling and vibrating to break up cells, and avoids the problems that the traditional sterile cell incubator is usually used for taking out the cells after the incubator door is opened and then checking, the sampling mode is troublesome, certain dust is easy to exist in the outside air without being purified, and the gas outside the incubator is easy to enter the incubator to generate great influence on the cells.
Description
Technical Field
The invention relates to the technical field of cell culture boxes, in particular to a portable sterile cell culture box.
Background
Cell culture chambers are commonly used to culture cells and are a method of providing sterility, proper temperature, ph and nutrient conditions to allow them to survive, grow, reproduce and maintain their primary structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or rarely differentiated multiple cells in a large scale, which is an indispensable link of the cloning technology.
Cell culture case is the indispensable technique of cultured cell, and traditional aseptic cell culture case is usually to take out the cell after opening the incubator door in the use and then inspect, and such sample mode is comparatively troublesome, does not obtain certain purification in the outside air moreover, also can have certain dust easily, makes the outer gaseous entering incubator of incubator inside easily, can produce very big influence to the cell.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a portable sterile cell culture box.
In order to achieve the purpose, the invention adopts the following technical scheme:
designing a portable sterile cell culture box, comprising a body, wherein two first cross rods which are symmetrically arranged are rotatably connected between the inner walls of the left side and the right side of the body, two chain wheels are respectively sleeved on the rod walls of the two first cross rods, chains are respectively connected between the two chain wheels which are adjacent up and down in a transmission manner, the left side wall of the body is fixedly connected with an installation frame, a motor is installed in the installation frame, the output shaft of the motor is fixedly connected with the left end of the first cross rod, a placing frame is uniformly arranged between the two chains, the left side wall and the right side wall of each group of placing frames are respectively and fixedly connected with a fixed rod, each group of fixed rods respectively penetrates through the adjacent chains, each group of fixed rods is rotatably connected with the adjacent chains, the left end of each group of fixed rods on the left side is respectively and fixedly connected with a cross plate, and the left side wall of each group of the cross plate is respectively, the inner wall of the left side of the body is provided with a movable groove matched with the first movable rod, the left side wall of each group of the cross plates is fixedly connected with a second movable rod close to the centers of the upper side and the lower side, the inner wall of the left side of the body is fixedly connected with two first vertical plates which are symmetrically arranged, each group of the second movable rods is slidably connected with the opposite sides of the two first vertical plates, each group of the placing frames is uniformly and fixedly connected with a second vertical plate, the opposite sides of the two adjacent second vertical plates are rotatably connected with the first circular plates, the front side wall and the rear side wall of each two adjacent first circular plates are fixedly connected with a second circular plate through transverse plates, the bottom of each group of the placing frames is uniformly and rotatably connected with a second vertical rod, the top end of each group of the second vertical rod is fixedly connected with an inclined plate, the top end of each group of the inclined plate is fixedly connected with a rotating roller, each group of the rotating rollers is positioned between the adjacent, each group the live-rollers all with adjacent first plectane contacts, each group the equal fixedly connected with household utensils in top of live-rollers, each group the inner chamber bottom left side department of placing the frame all rotates and is connected with first montant, each group the equal fixedly connected with fixed block in top of first montant, each group the even fixedly connected with mounting panel in bottom of placing the frame is adjacent all rotate on the mounting panel and be connected with the second horizontal pole, each group first montant and each group the first umbrella type gear of the equal fixedly connected with in bottom of second montant, each group all cup jointed on the pole wall of second horizontal pole with adjacent the second umbrella type gear of first umbrella type gear looks adaptation.
Preferably, the left side of the top of the body is rotatably connected with a first mounting rod, a second mounting rod is arranged in the first mounting rod, the outer wall of the second mounting rod is fixedly connected with two symmetrically arranged connecting blocks, a sliding groove matched with the second mounting rod and the connecting blocks is formed in the first mounting rod, a fixed frame is fixedly connected with the bottom end of the second mounting rod, the fixed frame is positioned right above the fixed block, the bottom of the fixed frame is provided with a fixed port matched with the fixed block, a first movable plate is arranged on the outer side of the fixed frame and rotatably connected with the fixed frame, a mounting block is arranged on the left side of the first mounting rod and fixedly connected with the body, the bottom of the mounting block is slidably connected with a push plate, and teeth are uniformly and fixedly connected with the bottom of the push plate, the bottom left side fixedly connected with fixed plate of department of installation piece, and the right side wall of fixed plate rotates and is connected with the gear, the gear with tooth intermeshing, the right side wall fixedly connected with second fly leaf of gear, fixedly connected with guiding axle on the left side wall of first fly leaf, set up on the right side wall of second fly leaf with the guide way of guiding axle looks adaptation, the top left side department of body is according to installing slow motor, and the output shaft of slow motor with the top fixed connection of first installation pole.
Preferably, the top of the body is uniformly and fixedly connected with observation mirrors, the top of each group of observation mirrors is provided with an opening, each group of observation mirrors is provided with a dropper in the opening, and each group of droppers is spliced with the adjacent opening.
Preferably, the right side of each group of the opening is provided with a baffle, the bottom of each group of the baffle is in threaded connection with a threaded rod, the bottom end of each group of the threaded rod is fixedly connected with the top of the body, and each group of the observation mirror is located right above the adjacent vessel.
Preferably, the bottom corner of body all sliding connection has the slide bar, each group the equal fixedly connected with base in bottom of slide bar, each group the pulley is all installed to the bottom of base.
Preferably, the left and right adjacent bases are fixedly connected with first connecting rods, the rod walls of the two first connecting rods are connected with two connecting plates in a sliding mode, springs are arranged between the connecting plates and the adjacent bases, and the springs are sleeved on the rod walls of the first connecting rods.
Preferably, each group of the connecting plates are far away from the adjacent side of the base and are movably connected with second connecting rods, and the other ends of the second connecting rods are movably connected with the bottom of the body.
Preferably, two warm lamps that the symmetry set up are installed to the rear side inner wall of body, two be provided with two ultraviolet lamp between the warm lamp, two ultraviolet lamp all with body fixed connection.
Preferably, the right side wall of body is close to the equal fixedly connected with purifying box of top department, the department installs the active carbon on the left side in the purifying box, the filter screen is installed on the right side department in the purifying box, the purifying box with body and outside homogeneous phase intercommunication, the front side swing joint of body has the dodge gate.
The invention provides a portable sterile cell culture box, which has the beneficial effects that:
1. the invention arranges the cylinder, the vessel, the slow motor and the dropper,
the output end of the air cylinder extends to drive the push plate to move towards the front side, the push plate moves to deflect through the tooth drive gear, the first movable plate can be driven to move downwards through the matching of the guide shaft and the guide groove, the first movable plate can be driven to drive the fixed frame to move downwards and be clamped at the top of the fixed block, the output shaft of the slow speed motor drives the first mounting rod to rotate and drive the second mounting rod to rotate, so that the fixed frame drives the first vertical rod to rotate through the fixed block, the second vertical rod is driven to rotate through the matching of the first umbrella-shaped gear and the second umbrella-shaped gear, the inclined plate can drive the vessel to deflect through the rotating roller to vibrate and disperse cells, the purpose of uniformly vibrating the cells is achieved, the purpose of facilitating sampling is achieved, then the cells in the vessel can be taken out through the dropper, the operation is facilitated, and the cell taking out, can effectively prevent the risk that the outside air enters the body to influence the cells.
2. The invention arranges the motor, the chain wheel, the chain and the placing frame, the output shaft of the motor drives the first cross rod to rotate through the rotating shaft, the chain wheel can be rotated and the chain can be driven to rotate, the chain can drive the cross plate to move through the fixed rod, the cross plate moves in the movable groove through the first movable rod, the cross plate moves and drives the second movable rod to slide between the two first vertical plates, the movable groove is arranged to achieve the effect of guiding the first movable rod, and the cross plate can be circularly moved through the matching of the first vertical plates, thereby leading the fixed rod to drive the placing frame to do circular motion, achieving the purpose of conveniently storing cells, effectively saving time, and can be to placing even rotation in the frame through the cooperation of sprocket and chain, can carry out even activity to the cell and be heated and disinfect, can the effectual survival rate that improves the cell.
Compared with the prior art, the invention is convenient for sampling and vibrating to break up cells, and avoids the problems that the traditional sterile cell incubator is usually used for taking out the cells after the incubator door is opened and then checking, the sampling mode is troublesome, certain dust is easy to exist in the outside air without being purified, and the gas outside the incubator is easy to enter the incubator to generate great influence on the cells.
Drawings
FIG. 1 is a sectional view showing a structure of a portable sterilized cell culture case according to the present invention;
FIG. 2 is a cross-sectional view of a placement frame structure with a portable sterile cell incubator according to the present invention;
FIG. 3 is an enlarged view of the portion A of FIG. 2 with a portable sterile cell incubator according to the present invention;
FIG. 4 is an enlarged view of the portion B of FIG. 2 with a portable sterile cell incubator according to the present invention;
FIG. 5 is a left sectional view of a second movable rod structure with a portable sterile cell incubator according to the present invention;
FIG. 6 is a right side view of a second movable plate structure with a portable sterile cell incubator according to the present invention.
FIG. 7 is a top view of a baffle structure with a portable sterile cell incubator according to the present invention;
FIG. 8 is a left side view of a fixing rod structure with a portable sterile cell incubator according to the present invention;
FIG. 9 is a top view of a second mounting bar structure with a portable sterile cell incubator in accordance with the present invention;
FIG. 10 is a schematic view of the structure of a fixing block and a fixing frame of a portable sterile cell incubator according to the present invention.
In the figure: the device comprises a body 1, a first cross bar 2, a chain wheel 3, a chain 4, a mounting frame 5, a motor 6, a placing frame 7, a fixed rod 8, a cross plate 9, a second movable rod 10, a movable groove 11, a first movable rod 12, a first vertical plate 13, a first vertical rod 14, a fixed block 15, a second vertical plate 16, a first circular plate 17, a transverse plate 18, a second circular plate 19, a second vertical rod 20, an inclined plate 21, a rotating roller 22, a vessel 23, a mounting plate 24, a second cross bar 25, a first umbrella-shaped gear 26, a second umbrella-shaped gear 27, a mounting block 28, a first mounting rod 29, a second mounting rod 30, a sliding groove 31, a connecting block 32, a fixed frame 33, a first movable plate 34, a guide shaft 35, a guide groove 36, a fixed plate 37, a push plate 38, a gear 39, an air cylinder 40, a slow motor 41, an observation mirror 42, an opening 43, a dropper 44, a threaded rod 45, a baffle 46, a tooth 47, an ultraviolet lamp 48, a, The device comprises a purification box 51, a sliding rod 52, a base 53, a first connecting rod 54, a connecting plate 55, a spring 56, a second connecting rod 57 and a second movable plate 58.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-10, a portable sterile cell incubator comprises a main body 1, two first cross rods 2 which are symmetrically arranged are rotatably connected between the inner walls of the left side and the right side of the main body 1, two chain wheels 3 are respectively sleeved on the rod walls of the two first cross rods 2, chains 4 are respectively connected between the two adjacent chain wheels 3 in a transmission manner, the left side wall of the main body 1 is fixedly connected with an installation frame 5, a motor 6 is installed in the installation frame 5, the output shaft of the motor 6 is fixedly connected with the left end of the first cross rod 2 on the upper side, a placing frame 7 is uniformly arranged between the two chains 4, the left side wall and the right side wall of each placing frame 7 are respectively and fixedly connected with a fixed rod 8, each group of fixed rods 8 respectively penetrates through the adjacent chains 4, each group of fixed rods 8 is rotatably connected with the adjacent chains 4, the left end of each group of fixed rods 8 on the left side is respectively and fixedly connected with a cross plate 9, and, the inner wall of the left side of the body 1 is provided with a movable groove 11 matched with the first movable rod 12, the left side wall of each group of cross plates 9 is fixedly connected with a second movable rod 10 close to the centers of the upper side and the lower side, the inner wall of the left side of the body 1 is fixedly connected with two first vertical plates 13 which are symmetrically arranged, each group of second movable rods 10 is slidably connected with the opposite sides of the two first vertical plates 13, an output shaft of the motor 6 drives the first cross rod 2 to rotate through a rotating shaft, the chain wheel 3 can rotate and drive the chain 4 to rotate, the chain 4 can drive the cross plates 9 to move through the fixed rods 8, the cross plates 9 move in the movable groove 11 through the first movable rods 12, the cross plates 9 move and drive the second movable rods 10 to slide between the two first vertical plates 13, the arrangement of the movable groove 11 achieves the effect of guiding the first movable rods 12, and the circular motion of the cross plates 9 can be realized through the matching of the first vertical plates 13, thereby make dead lever 8 drive place frame 7 and carry out cyclic motion, also can realize placing frame 7 each and carry out the adjusting position.
The inner part of each group of placing frames 7 is uniformly and fixedly connected with a second vertical plate 16, the opposite sides of the two adjacent second vertical plates 16 are respectively and rotatably connected with a first circular plate 17, the front side wall and the rear side wall of each two adjacent first circular plates 17 are respectively and fixedly connected with a second circular plate 19 through transverse plates 18, the bottom of each group of placing frames 7 is uniformly and rotatably connected with a second vertical rod 20, the top end of each group of second vertical rods 20 is fixedly connected with a sloping plate 21, the top end of each group of sloping plates 21 is respectively and fixedly connected with a rotating roller 22, each group of rotating rollers 22 is positioned between the adjacent first circular plates 17 and the second circular plates 19, each group of rotating rollers 22 is respectively contacted with the adjacent first circular plates 17, the top end of each group of rotating rollers 22 is respectively and fixedly connected with a vessel 23, the left side of the bottom of the inner cavity of each group of placing frames 7 is respectively and rotatably connected with a first vertical rod 14, the top end of each, the adjacent mounting plates 24 are rotatably connected with second cross bars 25, the bottom ends of the groups of first vertical bars 14 and the groups of second vertical bars 20 are fixedly connected with first umbrella-shaped gears 26, the wall of each group of second cross bars 25 is sleeved with second umbrella-shaped gears 27 matched with the adjacent first umbrella-shaped gears 26, the left side of the top of the body 1 is rotatably connected with a first mounting rod 29, a second mounting rod 30 is arranged in the first mounting rod 29, the outer wall of the second mounting rod 30 is fixedly connected with two symmetrically arranged connecting blocks 32, sliding grooves 31 matched with the second mounting rod 30 and the connecting blocks 32 are arranged in the first mounting rod 29, the bottom end of the second mounting rod 30 is fixedly connected with a fixed frame 33, the fixed frame 33 is positioned right above the upper fixed block 15, the bottom of the fixed frame 33 is provided with a fixed opening matched with the fixed block 15, the outer side of the fixed frame 33 is provided with a first movable plate 34, the first movable plate 34 is rotatably connected with the fixed frame 33, the left side of the first mounting rod 29 is provided with the mounting block 28, the mounting block 28 is fixedly connected with the body 1, the bottom of the mounting block 28 is slidably connected with the push plate 38, the bottom of the push plate 38 is uniformly and fixedly connected with the teeth 47, the left side of the bottom of the mounting block 28 is fixedly connected with the fixed plate 37, the right side wall of the fixed plate 37 is rotatably connected with the gear 39, the gear 39 is mutually meshed with the teeth 47, the right side wall of the gear 39 is fixedly connected with the second movable plate 58, the left side wall of the first movable plate 34 is fixedly connected with the guide shaft 35, the right side wall of the second movable plate 58 is provided with the guide groove 36 matched with the guide shaft 35, the left side of the top of the body 1 is provided with the slow motor 41, the output shaft of the slow motor 41 is fixedly connected with the top end of the first mounting rod 29, the, the top of each group of observation mirrors 42 is provided with an opening 43, each group of opening 43 is internally provided with a dropper 44, each group of droppers 44 is inserted with the adjacent opening 43, the right side of each group of opening 43 is provided with a baffle 46, the bottom of each group of baffle 46 is in threaded connection with a threaded rod 45, the bottom end of each group of threaded rod 45 is fixedly connected with the top of the body 1, each group of observation mirrors 42 is positioned right above the adjacent vessel 23, the output shaft of the slow motor 41 drives the first mounting rod 29 to rotate, the first mounting rod 29 drives the second mounting rod 30 to rotate through the connecting block 32, so that the fixing frame 33 drives the first vertical rod 14 to rotate through the fixing block 15, the leftmost first umbrella-shaped gear 26 can drive the second cross rod 25 to rotate through the leftmost second umbrella-shaped gear 27, the second cross rod 25 rotates to drive the second vertical rod 20 to rotate through the cooperation of the second umbrella-shaped gear 27 and the first umbrella-shaped gear 26, the inclined plate 21 can drive the vessel 23 to deflect through the rotating roller 22, the rotating roller 22 moves to deflect through the space between the first circular plate 17 and the second circular plate 19, the first circular plate 17 moves to move on the second vertical plate 16, the vessel 23 can deflect to vibrate and disperse cells at the moment, and then the cells in the vessel 23 can be taken out through the dropper 44, so that the purpose of facilitating sampling is achieved.
The bottom corners of the body 1 are slidably connected with slide bars 52, the bottom ends of the slide bars 52 are fixedly connected with bases 53, the bottoms of the bases 53 are respectively provided with a pulley, a first connecting rod 54 is fixedly connected between the left and right adjacent bases 53, two connecting plates 55 are respectively slidably connected on the rod walls of the two first connecting rods 54, springs 56 are respectively arranged between the connecting plates 55 and the adjacent bases 53, each group of springs 56 are respectively sleeved on the rod walls of the first connecting rods 54, one side of each group of connecting plates 55 far away from the adjacent bases 53 is respectively and movably connected with a second connecting rod 57, the other end of each group of second connecting rods 57 is movably connected with the bottom of the body 1, two symmetrically arranged heating lamps 49 are arranged on the inner wall of the rear side of the body 1, two ultraviolet lamps 48 are arranged between the two heating lamps 49, the two ultraviolet lamps 48 are respectively and fixedly connected with the body 1, a purification box 51 is fixedly connected at the position, close to the top, of the right side, activated carbon is installed to left side department in the purifying box 51, the filter screen is installed to right side department in the purifying box 51, purifying box 51 and body 1 and outside homogeneous phase intercommunication, the front side swing joint of body 1 has dodge gate 50, through second connecting rod 57, even board 55 and spring 56's cooperation, can realize that second connecting rod 57 passes through even board 55 motion extrusion spring 56, reach absorbing purpose, can be to placing frame 7 even rotation through the cooperation of sprocket 3 and chain 4, can carry out even activity to the cell and be heated and disinfect, can effectually improve the survival rate of cell, including a motor, an end cap, a controller, and a cover plate, cylinder 40, slow motor 41, ultraviolet lamp 48 and warm lamp 49 have control switch through external power cord electric connection respectively.
The working principle is as follows: when the invention is used, firstly, the ultraviolet lamp 48 is started to sterilize the inside of the body 1, the ultraviolet lamp 48 is started to preserve heat in the body 1, the aim of ventilation and filtration is achieved through the arrangement of the purifying box 51, when cells in the vessel 23 need to be sampled and observed, the motor 6 is started, the output shaft of the motor 6 drives the first cross rod 2 to rotate through the rotating shaft, the chain wheel 3 can be rotated and the chain 4 can be driven to rotate, the chain 4 can drive the cross plate 9 to move through the fixed rod 8, the cross plate 9 moves in the movable groove 11 through the first movable rod 12, the cross plate 9 moves and drives the second movable rod 10 to slide between the two first vertical plates 13, the arrangement of the movable groove 11 achieves the effect of guiding the first movable rod 12, and the cross plate 9 can be circularly moved through the matching of the first vertical plates 13, so that the fixed rod 8 drives the placing frame 7 to circularly move, when the placing frame 7 moves to the top, the motor 6 is turned off, the cylinder 40 is turned on, the output end of the cylinder 40 extends to drive the push plate 38 to move towards the front side, the push plate 38 moves to drive the gear 39 to deflect through the teeth 47, the second movable plate 58 can drive the first movable plate 34 to move downwards through the guide shaft 35, the guide shaft 35 moves to slide in the guide groove 36, the first movable plate 34 can drive the fixed frame 33 to move downwards and be clamped with the top of the fixed block 15, then the slow motor 41 is turned on, the output shaft of the slow motor 41 drives the first mounting rod 29 to rotate, the first mounting rod 29 drives the second mounting rod 30 to rotate through the connecting block 32, so that the fixed frame 33 drives the first vertical rod 14 to rotate through the fixed block 15, the leftmost first umbrella-shaped gear 26 can drive the second horizontal rod 25 to rotate through the leftmost second umbrella-shaped gear 27, and the cooperation of the first umbrella-shaped gear 26 drives the second vertical rod 20 to rotate through the cooperation of the second umbrella-shaped gear 27 and the tilting plate 21 can be rotated to drive the vessel 23 to deflect through the rotating roller 22, the rotating roller 22 can move to deflect through the space between the first circular plate 17 and the second circular plate 19, the first circular plate 17 moves to move on the second vertical plate 16, the vessel 23 can be deflected to vibrate and disperse cells at the moment, then the cells in the vessel 23 can be taken out through the dropper 44, after sampling is finished, the opening 43 at the top of the observation mirror 42 is sealed through the rotating baffle 46, and when the body 1 bumps, the second connecting rod 57 can move through the connecting plate 55 to extrude the spring 56, so that the shock absorption purpose is achieved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (9)
1. The portable sterile cell culture box comprises a body (1) and is characterized in that two first transverse rods (2) which are symmetrically arranged are rotatably connected between the inner walls of the left side and the right side of the body (1), two chain wheels (3) are sleeved on the rod walls of the two first transverse rods (2), a chain (4) is in transmission connection between every two adjacent chain wheels (3), the left side wall of the body (1) is fixedly connected with an installation frame (5), a motor (6) is installed in the installation frame (5), an output shaft of the motor (6) is fixedly connected with the left end of the first transverse rod (2), a placing frame (7) is uniformly arranged between the two chains (4), fixing rods (8) are fixedly connected to the left side wall and the right side wall of the placing frame (7) in each group, and the fixing rods (8) in each group penetrate through the adjacent chains (4), each group of the fixed rods (8) is rotatably connected with the adjacent chain (4), the left ends of the left groups of the fixed rods (8) are fixedly connected with a cross plate (9), the left side wall of each group of the cross plate (9) is close to the centers of the front side and the rear side, first movable rods (12) are fixedly connected with the left side inner wall of the body (1), movable grooves (11) matched with the first movable rods (12) are formed in the left side inner wall of the body (1), the left side wall of each group of the cross plate (9) is close to the centers of the upper side and the lower side, second movable rods (10) are fixedly connected with the left side inner wall of the body (1), two first vertical plates (13) which are symmetrically arranged are fixedly connected with each other, the second movable rods (10) are slidably connected with the opposite sides of the two first vertical plates (13), and second vertical plates (16) are uniformly and fixedly connected with the inside the placing frame (7), opposite sides of two adjacent second vertical plates (16) are rotatably connected with first circular plates (17), front and back side walls of the two adjacent first circular plates (17) are fixedly connected with second circular plates (19) through transverse plates (18), the bottom of each group of the placing frame (7) is uniformly rotatably connected with second vertical rods (20), the top end of each group of the second vertical rods (20) is fixedly connected with inclined plates (21), the top end of each group of the inclined plates (21) is fixedly connected with rotating rollers (22), each group of the rotating rollers (22) is positioned between the adjacent first circular plates (17) and the second circular plates (19), each group of the rotating rollers (22) is contacted with the adjacent first circular plates (17), the top end of each group of the rotating rollers (22) is fixedly connected with a vessel (23), and the left side of the bottom of an inner cavity of the placing frame (7) is rotatably connected with first vertical rods (14), each group the equal fixedly connected with fixed block in top (15), each group of first montant (14) the even fixedly connected with mounting panel (24) in bottom of placing frame (7), it is adjacent all rotate on mounting panel (24) and be connected with second horizontal pole (25), each group first montant (14) and each group the equal fixedly connected with first umbrella type gear (26) in bottom of second montant (20), each group all cup jointed on the pole wall of second horizontal pole (25) and adjacent second umbrella type gear (27) of first umbrella type gear (26) looks adaptation.
2. The portable sterile cell culture box according to claim 1, wherein a first mounting rod (29) is rotatably connected to the left side of the top of the body (1), a second mounting rod (30) is arranged in the first mounting rod (29), two symmetrically arranged connecting blocks (32) are fixedly connected to the outer wall of the second mounting rod (30), a sliding groove (31) matched with the second mounting rod (30) and the connecting blocks (32) is formed in the first mounting rod (29), a fixed frame (33) is fixedly connected to the bottom end of the second mounting rod (30), the fixed frame (33) is located above the fixed block (15) on the upper side, fixed openings matched with the fixed block (15) are formed in the bottom of the fixed frame (33), a first movable plate (34) is arranged on the outer side of the fixed frame (33), the utility model discloses a fixing frame for the automobile seat, including fixed frame (33), first fly leaf (34), first installation pole (29) and installation piece (28), and installation piece (28) with body (1) fixed connection, the bottom sliding connection of installation piece (28) has push pedal (38), and the even fixedly connected with tooth (47) in bottom of push pedal (38), the bottom left side department fixedly connected with fixed plate (37) of installation piece (28), and the right side wall of fixed plate (37) rotates and is connected with gear (39), gear (39) with tooth (47) intermeshing, the right side wall fixedly connected with second fly leaf (58) of gear (39), fixedly connected with guiding axle (35) on the left side wall of first fly leaf (34), set up on the right side wall of second fly leaf (58) with guiding groove (36) of guiding axle (35) looks adaptation, the left side of the top of the body (1) is provided with a slow motor (41), and an output shaft of the slow motor (41) is fixedly connected with the top end of the first mounting rod (29).
3. The portable sterile cell incubator according to claim 1, wherein the top of the main body (1) is uniformly and fixedly connected with observation mirrors (42), the top of each group of observation mirrors (42) is provided with an opening (43), each group of openings (43) is provided with a dropper (44), and each group of droppers (44) is inserted into the adjacent opening (43).
4. The portable sterile cell incubator according to claim 3, wherein a baffle (46) is arranged on the right side of each group of the openings (43), a threaded rod (45) is connected to the bottom of each group of the baffles (46) in a threaded manner, the bottom end of each group of the threaded rod (45) is fixedly connected with the top of the body (1), and each group of the observation mirrors (42) is positioned right above the adjacent vessel (23).
5. The portable sterile cell incubator according to claim 1, wherein the bottom corners of the body (1) are slidably connected with slide bars (52), the bottom ends of the slide bars (52) of each group are fixedly connected with bases (53), and the bottoms of the bases (53) of each group are provided with pulleys.
6. The portable sterile cell incubator according to claim 5, wherein a first connecting rod (54) is fixedly connected between two adjacent left and right bases (53), two connecting plates (55) are slidably connected to the rod walls of the two first connecting rods (54), a spring (56) is arranged between each group of connecting plates (55) and the adjacent base (53), and each group of springs (56) is sleeved on the rod wall of the first connecting rod (54).
7. The portable sterile cell incubator according to claim 6, wherein one side of each group of the connecting plates (55) far away from the adjacent base (53) is movably connected with a second connecting rod (57), and the other end of each group of the second connecting rods (57) is movably connected with the bottom of the body (1).
8. The portable sterile cell incubator according to claim 1, wherein two symmetrically arranged warm lamps (49) are installed on the inner wall of the rear side of the body (1), two ultraviolet lamps (48) are arranged between the two warm lamps (49), and the two ultraviolet lamps (48) are fixedly connected with the body (1).
9. The portable sterile cell culture box is characterized in that a purifying box (51) is fixedly connected to the position, close to the top, of the right side wall of the body (1), activated carbon is installed on the left side in the purifying box (51), a filter screen is installed on the right side in the purifying box (51), the purifying box (51) is communicated with the body (1) and the outer side, and a movable door (50) is movably connected to the front side of the body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110234705.XA CN112940936A (en) | 2021-03-03 | 2021-03-03 | Portable aseptic cell culture case has |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110234705.XA CN112940936A (en) | 2021-03-03 | 2021-03-03 | Portable aseptic cell culture case has |
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| CN112940936A true CN112940936A (en) | 2021-06-11 |
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| CN202110234705.XA Pending CN112940936A (en) | 2021-03-03 | 2021-03-03 | Portable aseptic cell culture case has |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113832002A (en) * | 2021-09-15 | 2021-12-24 | 中国计量大学 | A shake incubator for bacterial culture |
| CN115251043A (en) * | 2022-07-29 | 2022-11-01 | 冰山松洋生物科技(大连)有限公司 | Sample storage structure and liquid nitrogen biological container thereof |
-
2021
- 2021-03-03 CN CN202110234705.XA patent/CN112940936A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113832002A (en) * | 2021-09-15 | 2021-12-24 | 中国计量大学 | A shake incubator for bacterial culture |
| CN113832002B (en) * | 2021-09-15 | 2023-09-12 | 中国计量大学 | A shake incubator for bacterial cultivation |
| CN115251043A (en) * | 2022-07-29 | 2022-11-01 | 冰山松洋生物科技(大连)有限公司 | Sample storage structure and liquid nitrogen biological container thereof |
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