CN214853435U - Peucedanum praeruptorum callus regeneration device - Google Patents

Peucedanum praeruptorum callus regeneration device Download PDF

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CN214853435U
CN214853435U CN202121360158.1U CN202121360158U CN214853435U CN 214853435 U CN214853435 U CN 214853435U CN 202121360158 U CN202121360158 U CN 202121360158U CN 214853435 U CN214853435 U CN 214853435U
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placing
cultivation
chamber
sleeve
calli
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CN202121360158.1U
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Inventor
尹明华
黄兴明
蒋思盈
况怡
赖一汕
李欢
李舒悦
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Shangrao Normal University
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Shangrao Normal University
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Abstract

The utility model discloses a calli regeneration device of peucedanum praeruptorum, which comprises an incubator, wherein a partition board is arranged in the incubator, the incubator is divided into an incubation chamber and a power chamber by the partition board, and the incubation chamber is positioned above the power chamber; a placing mechanism for placing the cultivation test tubes is rotationally connected in the cultivation chamber, and the side wall of the cultivation chamber is communicated with a ventilation assembly; the outer wall of the cultivation room is provided with an operation opening for operation, the opposite side walls of the cultivation room are rotatably connected with one side of an opening and closing door, and the other sides of the two opening and closing doors are detachably connected; a first motor is fixedly connected in the power chamber, and an output shaft of the first motor is coupled with the placing mechanism. The utility model discloses can provide good condition of placing for the test tube of peucedanum praeruptorum callus regeneration culture, can hold more cultivation test tube in the device, be fit for the great peucedanum praeruptorum callus regeneration culture of scale.

Description

Peucedanum praeruptorum callus regeneration device
Technical Field
The utility model relates to a peucedanum praeruptorum cultivation technical field especially relates to a peucedanum praeruptorum callus regeneration device.
Background
The peucedanum praeruptorum is a perennial herbaceous plant and is a medicinal material in Shanghao district in Jiangxi province. Radix Peucedani is root of Peucedanum praeruptorum dunn of Umbelliferae, and is a common Chinese medicine. Has effects of dispelling pathogenic wind, clearing heat, lowering qi, and eliminating phlegm; for cold, cough, asthma and excessive phlegm due to respiratory tract infection, the peucedanum root is widely applied in the fields of treating wind-heat type cold, cough, asthma and excessive phlegm, etc.
In the cultivation of the peucedanum praeruptorum, a callus regeneration culture technology is generally adopted, a stem tip is inoculated into an MS stem tip culture medium test tube, a plantlet is cultivated in the test tube, and the plantlet is cultivated outdoors after passing virus detection. A device capable of placing a cultivation test tube is needed in the process from inoculation to plantlet growth, good conditions are provided for callus regeneration of the peucedanum praeruptorum, and the requirement of mass production is met.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a whiteflower hogfennel callus regeneration device to solve the problems of the prior art.
In order to achieve the above object, the utility model provides a following scheme: the utility model provides a calli regeneration device of peucedanum praeruptorum, which comprises an incubator, wherein a partition board is arranged in the incubator, the incubator is divided into an incubation chamber and a power chamber by the partition board, and the incubation chamber is positioned above the power chamber;
a placing mechanism for placing the cultivation test tubes is rotationally connected in the cultivation chamber, and the side wall of the cultivation chamber is communicated with a ventilation assembly; the outer wall of the cultivation room is provided with an operation opening for operation, the opposite side walls of the cultivation room are rotatably connected with one side of an opening and closing door, and the other sides of the two opening and closing doors are detachably connected;
a first motor is fixedly connected in the power chamber, and an output shaft of the first motor is coupled with the placing mechanism.
Preferably, the placing mechanism comprises a lifting part which is rotatably connected with the inner wall of the bottom end of the cultivation chamber, and the bottom end of the lifting part is in shaft connection with an output shaft of the first motor; the lifting part is provided with at least two groups of placing parts which are distributed along the vertical direction.
Preferably, the lifting part comprises an adjusting box which is rotatably connected with the cultivation room, and an adjusting component is installed in the adjusting box; the top end of the adjusting box is fixedly connected with a sleeve, and the tail end of the sleeve is rotationally connected with the inner wall of the top end of the culture chamber; the sleeve is internally provided with a lead screw, the top end of the lead screw is rotatably connected with the inner wall of the top end of the sleeve, and the bottom end of the lead screw is in shaft connection with the adjusting component.
Preferably, the adjusting assembly comprises a worm wheel and a worm which are rotatably connected with the adjusting box, and the worm wheel is meshed with the worm; a second motor is fixedly connected in the adjusting box, and an output shaft of the second motor is in shaft connection with the worm; the worm wheel is connected with the bottom end of the lead screw in a shaft mode.
Preferably, the placing part comprises a placing plate and a sliding sleeve which are fixedly sleeved outside the sleeve, the tray is arranged outside the sleeve, a plurality of placing holes are formed in the placing plate, a placing groove corresponding to the placing holes is formed in the top end of the tray, the tray is located below the placing plate, and the tray is in threaded connection with the lead screw.
Preferably, the threaded sleeve is sleeved on the screw rod and is provided with a threaded sleeve, the threaded sleeve is located in the sleeve, a long hole is formed in the sleeve, a connecting block matched with the width of the long hole is fixedly connected to the outer side of the threaded sleeve, and the tail end of the connecting block is fixedly connected to the inner side of the tray.
Preferably, still be provided with in the cultivation room with place the corresponding light filling disinfection subassembly of portion quantity, light filling disinfection subassembly include with the frame of cultivation indoor wall rigid coupling, just the frame is located the tray with place between the dish, be provided with a plurality of light filling lamps and a plurality of ultraviolet lamp in the frame, the light filling lamp with ultraviolet lamp arranges in turn.
Preferably, the opening and closing door is provided with an observation port, and high-transmittance glass matched with the observation port in size is fixedly connected in the observation port.
The utility model discloses a following technological effect:
the utility model discloses can provide good condition of placing for the test tube of peucedanum praeruptorum callus regeneration culture, can hold more cultivation test tube in the device, be fit for the great peucedanum praeruptorum callus regeneration culture of scale.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor.
FIG. 1 is an isometric view of a callus regeneration apparatus of Peucedanum japonicum Thunb of the present invention;
FIG. 2 is a front view of a callus regeneration apparatus of Peucedanum japonicum Thunb of the present invention;
FIG. 3 is a schematic structural view of a callus regeneration apparatus of Peucedanum japonicum Thunb of the present invention;
FIG. 4 is an enlarged view of A in FIG. 3;
FIG. 5 is a schematic structural diagram of an adjusting assembly according to the present invention;
FIG. 6 is a schematic structural view of a light supplementing and disinfecting module of the present invention;
wherein, 1 is incubator, 2 is exhaust fan, 3 is door, 4 is high transparent glass, 5 is baffle, 6 is the regulating box, 7 is the tray, 8 is the frame, 9 is for placing the dish, 10 is the sleeve, 11 is the lead screw, 12 is the light filling lamp, 13 is ultraviolet lamp, 14 is the worm, 15 is the worm wheel, 16 is first motor, 17 is the thread bush, 18 is the second motor, 19 is the connecting block.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.
The utility model provides a calli regeneration device of peucedanum praeruptorum, which comprises an incubator 1, wherein a baffle 5 is arranged in the incubator 1, the incubator 1 is divided into an incubation chamber and a power chamber by the baffle 5, and the incubation chamber is positioned above the power chamber;
a placing mechanism for placing the cultivation test tubes is rotationally connected in the cultivation chamber, and the side wall of the cultivation chamber is communicated with a ventilation assembly; the outer wall of the cultivation room is provided with an operation opening for operation, the opposite side walls of the cultivation room are rotatably connected with one side of the opening and closing door 3, and the other sides of the two opening and closing doors 3 are detachably connected;
a first motor 16 is fixedly connected in the power chamber, and an output shaft of the first motor 16 is coupled with the placing mechanism.
Further, ventilation unit includes exhaust fan 2, all sets up the exhaust hole with 2 size looks adaptations of exhaust fan on the relative lateral wall of cultivation room, and every downthehole equal rigid coupling of exhaust has a row of fan 2. The air exhaust speed is controlled by controlling the rotating speed of the exhaust fan 2, so that the temperature in the cultivation room is controlled, and the overhigh temperature in the cultivation room is avoided; meanwhile, the exhaust fan 2 can also exhaust waste gas generated in the regeneration process of the calli of the peucedanum praeruptorum so as to be beneficial to the regeneration of the calli of the peucedanum praeruptorum.
Furthermore, one side of the opening and closing door 3 is rotatably connected with the side wall of the cultivation room through a hinge, and the other sides of the two opening and closing doors 3 are detachably connected through a bolt.
Further, in order to open and close the opening and closing door 3 conveniently, a handle is fixedly connected to the outer side of the opening and closing door 3.
Further, the placing mechanism comprises a lifting part which is rotatably connected with the inner wall of the bottom end of the cultivation chamber, and the bottom end of the lifting part is coupled with an output shaft of the first motor 16; the lifting part is provided with at least two placing parts which are distributed along the vertical direction.
Further, the lifting part comprises an adjusting box 6 which is rotatably connected with the culture chamber, and an adjusting component is arranged in the adjusting box 6; the top end of the adjusting box 6 is fixedly connected with a sleeve 10, and the tail end of the sleeve 10 is rotationally connected with the inner wall of the top end of the cultivation chamber; be provided with lead screw 11 in the sleeve 10, the top of lead screw 11 rotates with sleeve 10 top inner wall to be connected, and the bottom and the adjusting part coupling of lead screw 11.
Further, the adjusting assembly comprises a worm wheel 15 and a worm 14 which are rotatably connected with the adjusting box 6, and the worm wheel 15 is meshed with the worm 14; a second motor 18 is fixedly connected in the adjusting box 6, and an output shaft of the second motor 18 is coupled with the worm 14; the worm wheel 15 is coupled to the bottom end of the lead screw 11.
Furthermore, a rotating seat is fixedly connected to the inner wall of the bottom end of the adjusting box 6, and the worm 14 is rotatably connected with the adjusting box 6 through the rotating seat.
Further, the placing part comprises a placing plate 9 fixedly sleeved outside the sleeve 10, a tray 7 slidably sleeved outside the sleeve 10, a plurality of placing holes are formed in the placing plate 9, a placing groove corresponding to the placing holes is formed in the top end of the tray 7, the tray 7 is located below the placing plate 9, and the tray 7 is in threaded connection with the screw rod 11. Second motor 18 rotates, drives tray 7 and goes up and down, and then drives and cultivates the test tube and go up and down, not only can make the callus that acts on in the cultivation test tube that light filling lamp 12 and ultraviolet lamp 13 can be better, moreover when needs take out the cultivation test tube can be more convenient will cultivate the test tube and take out.
Further, the threaded sleeve is sleeved with a threaded sleeve 17 on the screw rod 11, the threaded sleeve 17 is located in the sleeve 10, a long hole is formed in the sleeve 10, a connecting block 19 matched with the width of the long hole is fixedly connected to the outer side of the threaded sleeve 17, and the tail end of the connecting block 19 is fixedly connected with the inner side of the tray 7.
Further, still be provided with in the cultivation room with the corresponding light filling disinfection subassembly of placing portion quantity, light filling disinfection subassembly include with the frame 8 of cultivation indoor wall rigid coupling, and frame 8 is located tray 7 and places between the dish 9, is provided with a plurality of light filling lamps 12 and a plurality of ultraviolet lamp 13 in the frame 8, and light filling lamps 12 and ultraviolet lamp 13 arrange in turn. The light supplement lamp 12 not only can supplement light to the calli of the peucedanum praeruptorum in the culture test tube to promote the production of the calli, but also can heat the culture room, so that the culture room can be always at a temperature suitable for the regeneration of the calli of the peucedanum praeruptorum. Ultraviolet lamp 13 can disinfect to the culture test tube, avoids because the bacterium gets into the culture test tube and pollutes the callus after primary inoculation and follow-up supplementary culture solution, causes the death of callus.
Further, in order to enable the light supplement lamp 12 and the ultraviolet lamp 13 to better culture the regenerated tissue in the test tube, the frame 8 is an annular frame with a gap, and the frame 8 surrounds the outer side of the placing disc 9.
Furthermore, in order to observe the regeneration condition of the callus more conveniently, an observation port is formed in the opening and closing door 3, and high-transparent glass 4 matched with the size of the observation port is fixedly connected in the observation port.
The specific implementation mode is as follows:
put into placing the dish 9 through placing the hole with the cultivation test tube of inoculating good peucedanum praeruptorum callus, 16 operation of first motor drive regulating box 6 rotations, and then drive sleeve 10 and rotate, and then drive placing dish 9 and tray 7 on the sleeve 10 and rotate, make that operating personnel can be more convenient put into the hole of placing that is in the rear with the cultivation test tube, tray 7 sets up the standing groove with cultivation test tube bottom looks adaptation, the standing groove can steadily drag the cultivation test tube, avoid because rock and lead to the cultivation test tube damaged.
Control second motor 18 and rotate after the completion is placed to the cultivation test tube, second motor 18 drives worm 14 and rotates, worm 14 drives the worm wheel 15 rotation of meshing with it, and then drive the lead screw 11 rotation with 15 coupling of worm wheel, because the restriction thread bush 17 of slot hole and connecting block 19 descends and then drives the tray 7 of rigid coupling with it to descending, drive the cultivation test tube to descending, make the callus that acts on in the cultivation test tube that light filling lamp 12 and ultraviolet lamp 13 can be better, control second motor 18 reversal tray 7 and drive the cultivation test tube and rise when needs take out the cultivation test tube and be convenient for take out the cultivation test tube.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description of the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
The above-mentioned embodiments are only intended to describe the preferred embodiments of the present invention, but not to limit the scope of the present invention, and those skilled in the art should also be able to make various modifications and improvements to the technical solution of the present invention without departing from the spirit of the present invention, and all such modifications and improvements are intended to fall within the scope of the present invention as defined in the appended claims.

Claims (8)

1. A regeneration device of calli of peucedanum praeruptorum, which is characterized in that: the cultivation device comprises an incubator (1), wherein a partition plate (5) is arranged in the incubator (1), the incubator (1) is divided into an incubation chamber and a power chamber by the partition plate (5), and the incubation chamber is positioned above the power chamber;
a placing mechanism for placing the cultivation test tubes is rotationally connected in the cultivation chamber, and the side wall of the cultivation chamber is communicated with a ventilation assembly; the outer wall of the cultivation room is provided with an operation opening for operation, the opposite side walls of the cultivation room are rotatably connected with one side of an opening and closing door (3), and the other sides of the two opening and closing doors (3) are detachably connected;
a first motor (16) is fixedly connected in the power chamber, and an output shaft of the first motor (16) is in shaft connection with the placing mechanism.
2. The regeneration device of calli of peucedanum praeruptorum as claimed in claim 1, wherein: the placing mechanism comprises a lifting part which is rotationally connected with the inner wall of the bottom end of the cultivation chamber, and the bottom end of the lifting part is coupled with an output shaft of the first motor (16); the lifting part is provided with at least two groups of placing parts which are distributed along the vertical direction.
3. The regeneration device of calli of peucedanum praeruptorum as claimed in claim 2, wherein: the lifting part comprises an adjusting box (6) which is rotatably connected with the culture chamber, and an adjusting component is arranged in the adjusting box (6); the top end of the adjusting box (6) is fixedly connected with a sleeve (10), and the tail end of the sleeve (10) is rotatably connected with the inner wall of the top end of the culture chamber; the adjustable screw rod is characterized in that a screw rod (11) is arranged in the sleeve (10), the top end of the screw rod (11) is rotatably connected with the inner wall of the top end of the sleeve (10), and the bottom end of the screw rod (11) is in shaft connection with the adjusting assembly.
4. The regeneration device of calli of peucedanum praeruptorum as claimed in claim 3, wherein: the adjusting assembly comprises a worm wheel (15) and a worm (14) which are rotatably connected with the adjusting box (6), and the worm wheel (15) is meshed with the worm (14); a second motor (18) is fixedly connected in the adjusting box (6), and an output shaft of the second motor (18) is in shaft connection with the worm (14); the worm wheel (15) is in shaft connection with the bottom end of the lead screw (11).
5. The regeneration device of calli of peucedanum praeruptorum as claimed in claim 3, wherein: the placing part comprises a placing disc (9) fixedly sleeved on the outer side of the sleeve (10) and a tray (7) slidably sleeved on the outer side of the sleeve (10), a plurality of placing holes are formed in the placing disc (9), a placing groove corresponding to the placing holes is formed in the top end of the tray (7), the tray (7) is located below the placing disc (9), and the tray (7) is in threaded connection with the lead screw (11).
6. The regeneration device of calli of peucedanum praeruptorum as claimed in claim 5, wherein: threaded sleeve is equipped with thread bush (17) on lead screw (11), just thread bush (17) are located in sleeve (10), the slot hole has been seted up on sleeve (10), thread bush (17) outside rigid coupling have with connecting block (19) of slot hole width looks adaptation, the end of connecting block (19) with tray (7) inboard rigid coupling.
7. The regeneration device of calli of peucedanum praeruptorum as claimed in claim 5, wherein: cultivate indoor still be provided with place the corresponding light filling disinfection subassembly of portion quantity, light filling disinfection subassembly include with frame (8) of indoor wall rigid coupling of cultivating, just frame (8) are located tray (7) with place between the dish (9), be provided with a plurality of light filling lamps (12) and a plurality of ultraviolet lamp (13) in frame (8), light filling lamp (12) with ultraviolet lamp (13) are arranged in turn.
8. The regeneration device of calli of peucedanum praeruptorum as claimed in claim 1, wherein: an observation port is formed in the opening and closing door (3), and high-transmittance glass (4) matched with the observation port in size is fixedly connected in the observation port.
CN202121360158.1U 2021-06-18 2021-06-18 Peucedanum praeruptorum callus regeneration device Active CN214853435U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115380830A (en) * 2022-10-08 2022-11-25 浙江大学 Propagation method of Peucedanum praeruptorum dunn adventitious bud

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115380830A (en) * 2022-10-08 2022-11-25 浙江大学 Propagation method of Peucedanum praeruptorum dunn adventitious bud

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