CN216438394U

CN216438394U - Cell transfer incubator

Info

Publication number: CN216438394U
Application number: CN202122408228.2U
Authority: CN
Inventors: 鹿娟; 毛小敏; 肖招英
Original assignee: First Affiliated Hospital of Zhejiang University School of Medicine
Current assignee: First Affiliated Hospital of Zhejiang University School of Medicine
Priority date: 2021-10-08
Filing date: 2021-10-08
Publication date: 2022-05-06
Anticipated expiration: 2031-10-08

Abstract

The cell transferring incubator comprises a box body with an opening at the front side, wherein a vertical plate is fixed in the box body, the vertical plate divides an inner cavity of the box body into a left cultivation cavity and a right transferring cavity, two box doors are hinged to the front side of the box body and are in a split state, the left box door is used for sealing the cultivation cavity, and the right box door is used for sealing the transferring cavity; in conclusion, two box doors of the new technology are controlled to be opened and closed through the foot switch, so that the situation that the two box doors are repeatedly opened by hands during cell transfer to be infected with bacteria on the box doors is avoided, and the pollution possibility is reduced. The cell culture box and the cell transfer box are mutually matched for transferring in the new technology, so that the possibility of cell pollution when a cell culture dish is taken every time is avoided, and the working efficiency is improved; the new technology avoids the operation trouble caused by repeated disinfection in the cell transferring process, reduces the possibility of cell pollution and increases the working efficiency.

Description

Cell transfer incubator

Technical Field

The utility model belongs to the technical field of medical treatment, and particularly relates to a cell transfer incubator.

Background

Cell culture is an important experimental operation in basic research, and the reaction of cells to experimental conditions in an in vitro environment can be researched by culturing the cells and applying the experimental conditions, so that the process of occurrence and development of diseases and a treatment method can be researched. The current cell culture method is a method for simulating in-vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to allow cells to survive, grow and reproduce in a culture box and maintain main structures and functions. At present, when in vitro cell culture, the cell culture device is placed in a cell culture container (a culture dish, a culture bottle and the like), then the cell culture container is placed in a cell culture box, the cell culture box can provide the temperature, the carbon dioxide concentration, the humidity and the sterile environment required by cell growth, when in general cell culture, cell culture solution needs to be replaced at least once every day, the replacement of the culture solution can remove waste generated by cell metabolism and add new culture solution, the cells are provided with required nutrient substances and the like, at present, the replacement of the cells or the addition of other experimental conditions (such as drugs for inhibiting or promoting cell growth and the like) need to be completed in a clean bench, at present, the clean bench and the cell culture box are two separated devices, so the cell culture device needs to be placed in a container and transported to the clean bench for experimental operation, and during transportation, repeated alcohol disinfection is needed, cell contamination easily occurs during the transfer process, and further the experiment fails. At present, most of cell culture boxes are of an internal cavity structure, then cell culture containers are stacked in the culture box, the internal space of the cell culture containers is inconvenient to be fully utilized, the cell culture boxes are not specially equipped with cell transfer containers at present, the cell culture containers are usually specially used after being disinfected by a non-special container, the non-special container easily causes cell pollution, and the pollution risk is increased.

SUMMERY OF THE UTILITY MODEL

The utility model provides a cell transfer incubator for solving the defects in the prior art and reducing the risk of transfer pollution.

In order to solve the technical problems, the utility model adopts the following technical scheme: the cell transferring incubator comprises a box body with an opening at the front side, wherein a vertical plate is fixed in the box body, the vertical plate divides an inner cavity of the box body into a left cultivation cavity and a right transferring cavity, two box doors are hinged to the front side of the box body and are in a split state, the left box door is used for sealing the cultivation cavity, and the right box door is used for sealing the transferring cavity;

the cell culture box is characterized in that a plurality of suspension rods are fixed on the inner wall of the rear side of the box body culture cavity, all the suspension rods are arranged in a rectangular array, two adjacent suspension rods on the left side and the right side form a group of support frames, a cell culture box is supported on each group of support frames, the cell culture box is of a box body structure with an open top, support bar plates are arranged on the left side and the right side of the top of the cell culture box, the cell culture box is supported and placed on the two suspension rods of the support frames through the two support bar plates, a marking plate is arranged on the box wall on the front side of the cell culture box, and two handles are arranged on the top of the cell culture box;

three partition plates are fixed in a transfer cavity of the box body and arranged side by side up and down, a cell transfer box is placed on each partition plate, the cell transfer box is of a box body structure with an open top, the top of the cell transfer box is hinged with a box cover, a handle is arranged on the box cover, and a first ultraviolet lamp is installed inside the box cover;

the bottom of a culture cavity of the box body is provided with a temperature control unit, a temperature sensor and a carbon dioxide concentration sensor are arranged in the culture cavity, the top of the box body is provided with a control box, a controller is arranged in the control box, a display screen is arranged on the control box, the controller is respectively connected with the display screen, the temperature sensor and the carbon dioxide concentration sensor through circuits, and the side part of the culture cavity of the box body is connected with a vent pipe;

and a second ultraviolet lamp is arranged in the transfer cavity of the box body and is used for sterilizing the transfer cavity.

The outer box wall of the cell culture box is uniformly provided with a plurality of vent holes.

The top of the box body is provided with two groups of electric control door opening assemblies which are respectively used for controlling the opening and closing of two box doors, wherein the box doors are hinged on the box body through rotating shafts, each electric control door opening assembly comprises a motor arranged at the top of the box body, a main bevel gear is arranged on a main shaft of the motor, a slave bevel gear is arranged at the upper end of each rotating shaft, and the slave bevel gears are meshed with the main bevel gears;

the motors of the two groups of electric control door opening assemblies are respectively controlled by the foot switches.

By adopting the technical scheme, the utility model has the following beneficial effects: two chamber doors of new technology are opened and closed through foot switch control, avoid opening the door repeatedly with the hand when the cell is transported, are infected with the bacterium on the case door, reduce the pollution possibility. The cell culture box and the cell transfer box are mutually matched for transferring in the new technology, so that the possibility of cell pollution when a cell culture dish is taken every time is avoided, and the working efficiency is improved; the new technology avoids the operation trouble caused by repeated disinfection in the cell transferring process, reduces the possibility of cell pollution and increases the working efficiency.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic diagram of the structure of the cell culture cassette;

FIG. 3 is a schematic diagram of the structure of the cell transport cassette;

fig. 4 is a schematic structural view of an electrically controlled door opening assembly.

Detailed Description

As shown in fig. 1-4, the cell transfer incubator of the utility model comprises a case body 1 with an opening at the front side, wherein a vertical plate 2 is fixed in the case body 1, the vertical plate 2 divides the inner cavity of the case body 1 into a left cultivation cavity and a right transfer cavity, the front side of the case body 1 is hinged with two case doors 3, the two case doors 3 are in a split state, the left case door 3 is used for sealing the cultivation cavity, and the right case door 3 is used for sealing the transfer cavity;

a plurality of suspension rods 4 are fixed on the inner wall of the rear side of a culture cavity of the box body 1, all the suspension rods 4 are arranged in a rectangular array, a group of support frames is formed by two suspension rods 4 which are adjacent left and right, a cell culture box 5 is supported on each group of support frames, the cell culture box 5 is of a box body structure with an open top, support strip plates 6 are arranged on the left and right sides of the top of the cell culture box 5, the cell culture box 5 is supported and placed on the two suspension rods 4 of the support frames through the two support strip plates 6, a marking plate 7 is arranged on the box wall of the front side of the cell culture box 5, and two handles 8 are arranged on the top of the cell culture box 5;

three partition plates 9 are fixed in a transfer cavity of the box body 1, the three partition plates 9 are arranged side by side up and down, a cell transfer box 10 is placed on each partition plate 9, the cell transfer box 10 is of a box body structure with an open top, the top of the cell transfer box 10 is hinged with a box cover 11, a handle 12 is arranged on the box cover 11, and a first ultraviolet lamp 13 is installed inside the box cover 11;

the bottom of a culture cavity of the box body 1 is provided with a temperature control unit, a temperature sensor and a carbon dioxide concentration sensor are arranged in the culture cavity, the top of the box body 1 is provided with a control box, a controller is arranged in the control box, a display screen 14 is arranged on the control box, the controller is respectively connected with the display screen 14, the temperature sensor and the carbon dioxide concentration sensor through circuits, and the side part of the culture cavity of the box body 1 is connected with a vent pipe 21;

a second ultraviolet lamp 15 is installed in the transferring cavity of the box body 1, and the second ultraviolet lamp 15 is used for sterilizing and disinfecting the transferring cavity.

The outer box wall of the cell culture box 5 is uniformly provided with a plurality of vent holes 16.

The top of the box body 1 is provided with two groups of electric control door opening assemblies which are respectively used for controlling the opening and closing of two box doors 3, wherein the box doors 3 are hinged on the box body 1 through a rotating shaft 17, each electric control door opening assembly comprises a motor 18 arranged at the top of the box body 1, a main bevel gear 19 is arranged on a main shaft of the motor 18, a slave bevel gear 20 is arranged at the upper end of the rotating shaft 17, and the slave bevel gear 20 is meshed with the main bevel gear 19;

the motors 18 of the two groups of electrically controlled door opening assemblies are respectively controlled by foot switches.

The novel technology is characterized in that two independent culture chambers and a transfer chamber are arranged in a box body 1, the culture chambers are used for placing cell culture boxes 5 through a plurality of built-in suspension rods 4, a marking plate 7 on each cell culture box 5 is used for marking information such as cell culture types, time and the like, vent holes 16 are uniformly distributed in the side wall of each cell culture box, gas in the culture chambers can conveniently circulate, and cell culture dishes can be placed in the cell culture boxes 5 for culture; in addition, a display screen 14 is arranged at the top of the box body 1, a temperature sensor and a carbon dioxide concentration sensor are used for monitoring the temperature and the carbon dioxide concentration in the culture cavity in real time, displaying is carried out on the display screen 14, the internal temperature of the culture cavity is adjusted through a temperature control unit, carbon dioxide is introduced into the ventilation pipe 21, and the internal carbon dioxide concentration is adjusted;

the transfer cavity is used for placing the cell transfer box 10, when cells are transferred, the foot switch is trodden after the gloves are worn, the right box door 3 is opened, then the sterilized cell transfer box 10 is taken out, then the left box door 3 is opened by trodden the foot switch, the cell culture box 5 is directly taken out from the culture cavity and is placed in the cell transfer box 10, wherein the cell culture box 5 is supported and placed on the edge of the top of the cell transfer box 10 through the two stay plates 6, then the cell transfer can be carried out by closing the two box doors 3, the cell culture box 5 is sterilized and then is placed in the culture cavity after the cell transfer is finished, and the cell transfer box 10 is placed in the transfer cavity;

at 11 inside first ultraviolet lamps 13 that are provided with of lid of cell transportation box 10, when not placing cell culture box 5, open first ultraviolet lamp 13 and can transport box 10 inside disinfecting to the cell, when need not carrying out the cell and transport, transport box 10 and place the transportation intracavity with the cell, transport the intracavity and be provided with second ultraviolet lamp 15, wherein do not place the cell and transport box 10 when, can open second ultraviolet lamp 15 and disinfect.

The present embodiment is not intended to limit the shape, material, structure, etc. of the present invention in any way, and any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims

1. Cell transfer incubator, its characterized in that: the box comprises a box body with an opening at the front side, wherein a vertical plate is fixed in the box body and divides an inner cavity of the box body into a left culture cavity and a right transfer cavity;

and a second ultraviolet lamp is arranged in the transfer cavity of the box body and is used for sterilizing and disinfecting the transfer cavity.

2. The cell transfer incubator of claim 1, wherein: the outer box wall of the cell culture box is uniformly provided with a plurality of vent holes.

3. The cell transfer incubator of claim 1, wherein: the top of the box body is provided with two groups of electric control door opening assemblies which are respectively used for controlling the opening and closing of two box doors, wherein the box doors are hinged on the box body through rotating shafts, each electric control door opening assembly comprises a motor arranged at the top of the box body, a main bevel gear is arranged on a main shaft of the motor, a slave bevel gear is arranged at the upper end of each rotating shaft, and the slave bevel gears are meshed with the main bevel gears;