CN110724638A - A culture apparatus for embryo culture - Google Patents

A culture apparatus for embryo culture Download PDF

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Publication number
CN110724638A
CN110724638A CN201911176087.7A CN201911176087A CN110724638A CN 110724638 A CN110724638 A CN 110724638A CN 201911176087 A CN201911176087 A CN 201911176087A CN 110724638 A CN110724638 A CN 110724638A
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cavity
gear
transmission
shaft
culture
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吴美君
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Yuyao Shengpu Biotechnology Co Ltd
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Yuyao Shengpu Biotechnology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a culture device for embryo culture, which comprises a box body, wherein a lower pressing cavity is arranged at the upper left of the box body, an extrusion cavity is arranged at the lower right of the lower pressing cavity, a bevel gear cavity is arranged below the lower pressing cavity, a transmission cavity is arranged below the bevel gear cavity, a culture cavity is arranged above the transmission cavity, a pressing plate is arranged in the extrusion cavity, the left end and the right end of the pressing plate are abutted against the left end wall and the right end wall of the extrusion cavity, nutrient solution is arranged below the pressing plate, a spraying sieve plate is arranged below the nutrient solution and is fixedly arranged on the lower end wall of the extrusion cavity, the culture device for embryo culture comprises a box body, a lower pressing cavity is arranged at the upper left of the box body, an extrusion cavity is arranged at the lower right of the lower pressing cavity, a bevel gear cavity is arranged below the lower pressing cavity, a transmission cavity is arranged above the transmission cavity, and a pressing plate is arranged in the extrusion cavity.

Description

A culture apparatus for embryo culture
Technical Field
The invention relates to the field of embryo culture, in particular to a culture device for embryo culture.
Background
Present embryo culture all is artifical and carries out the injection of nutrient solution to the culture dish, laboratory staff continues to grow in transferring the embryonated egg from the fertilization liquid to the droplet of culture dish and develops, freezing or embryo transfer until the third day embryo, wherein when dripping the nutrient solution to the embryo, this wherein, need guarantee that the embryo culture dish of each difference embryo culture liquid can not make mistakes, otherwise can cause the influence to the growth of embryo, because manual operation hardly guarantees that injection at every turn is all unanimous and even, can't guarantee that each culture solution droplet volume is the same, so need design out mechanical device and replace this work.
Disclosure of Invention
The invention aims to provide a culture device for embryo culture, which overcomes the defect that the injection of nutrient solution is inconsistent during the embryo culture at the present stage, and the possibility that the embryo is influenced by the outside because of manual operation when the nutrient solution is instilled into a culture dish every time.
The invention is realized by the following technical scheme.
The invention discloses a culture device for embryo cultivation, which comprises a box body, wherein a lower pressing cavity is arranged at the upper left of the box body, an extrusion cavity is arranged at the lower right of the lower pressing cavity, a bevel gear cavity is arranged below the lower pressing cavity, a transmission cavity is arranged below the bevel gear cavity, a culture cavity is arranged above the transmission cavity, a pressing plate is arranged in the extrusion cavity, the left end and the right end of the pressing plate are abutted against the left end wall and the right end wall of the extrusion cavity, nutrient solution is arranged below the pressing plate, a spraying sieve plate is arranged below the nutrient solution, and the spraying sieve plate is fixedly arranged on the lower end wall of the extrusion cavity;
the right side of the nutrient solution in the extrusion cavity is communicated with a nutrient solution storage cavity, the nutrient solution storage cavity is internally provided with nutrient solution, the nutrient solution storage cavity is arranged on the right side of the lower pressure cavity, an injection hole is formed above the nutrient solution storage cavity, a lower pressure rod is fixedly arranged above the pressure plate, the upper end of the lower pressure rod is fixedly connected with a connecting rod, the left side of the connecting rod is fixedly provided with a fixed sliding block, the fixed sliding block at the left end of the connecting rod is fixedly arranged in a rotary table sliding groove, the rotary table sliding groove is positioned in a rotary table, the rotary table is fixedly arranged on a rotary shaft, and the front end and the rear end of the rotary shaft are respectively and rotatably arranged in;
the carousel rear side is equipped with right turn gear, right turn gear fixed mounting is in the axis of rotation, right turn gear left side gear engagement is connected with the left-hand gear, left-hand gear fixed mounting is on the transmission shaft, the transmission shaft front and back end rotates respectively to be installed in the end wall around the chamber pushes down, left-hand gear front side is equipped with belt pulley, belt pulley fixed mounting is on the transmission shaft on going up, it is equipped with cultivates the subassembly to go up the belt pulley below, it is equipped with humidity control chamber to push down the chamber right side, humidity control chamber below is equipped with the cavity, the humidity control intracavity is equipped with humidity control assembly, humidity control chamber below is equipped with carbon dioxide control chamber, carbon dioxide control intracavity is equipped with oxygen control assembly.
Further, the culture assembly comprises a lower belt gear, the lower belt gear is connected with an upper belt wheel through a belt, the lower belt gear is fixedly installed on a belt shaft, the front end and the rear end of the belt shaft are respectively and rotatably installed in the front end wall and the rear end wall of the helical gear cavity, a left transmission helical gear is arranged on the rear side of the lower belt gear, the left transmission helical gear is fixedly installed on the belt shaft, a right transmission helical gear is arranged on the front side of the left transmission helical gear and is meshed with the left transmission helical gear, the right transmission helical gear is fixedly installed on a helical gear shaft, the right end of the helical gear shaft is rotatably installed in the right wall end of the helical gear cavity, an upper helical gear is arranged on the right side of the right transmission helical gear, the upper helical gear is fixedly installed on the helical gear shaft, a lower helical gear is arranged below the upper helical gear, and the, the gear transmission shaft is rotatably arranged between the bevel gear cavity and the transmission cavity, the lower end of the gear transmission shaft is in power connection with a motor fixedly arranged in the bottom wall of the transmission cavity, a sector wheel is arranged in the transmission cavity, the sector wheel is fixedly arranged on the gear transmission shaft, a rotating rod is fixedly connected below the sector wheel, a boss is arranged at the upper right part of the rotating rod, an intermittent wheel is arranged at the right side of the fan-shaped wheel, an intermittent shaft is rotatably arranged between the carbon dioxide control cavity and the transmission cavity, the lower end of the intermittent shaft extends into the transmission cavity and is fixedly connected with the intermittent wheel, four clamping grooves are arranged in the intermittent wheel, a supporting disc is arranged above the intermittent wheel, the upper end of the intermittent shaft extends into the culture cavity and is fixedly connected with the supporting disc, four placing grooves are formed in the supporting disc, and four culture dishes are arranged on the four placing grooves.
Further, the humidity control assembly comprises a water tank, clear water is arranged in the water tank, the water tank is fixedly arranged on the lower wall end of the humidity control cavity, a cover plate is arranged above the water tank, two left fixing rods are arranged on the front and back of the left side of the cover plate, the lower ends of the two left fixing rods are fixedly arranged on the outer box body at the lower end of the water tank, a fixing spring is arranged on the left side of the left fixing rod, the left side of the fixing spring is fixedly arranged on the water tank, two right fixing rods are arranged on the front and back of the right side of the cover plate, the lower ends of the two right fixing rods are fixedly arranged on the outer box body at the lower end of the water tank, a connecting rod is fixedly connected between the two right fixing rods, a rope is fixedly connected on the right side of the connecting rod, a rope cavity is arranged on the right side of the humidity control cavity, the utility model discloses a tensile magnetism board, including left rope sheave, right rope sheave, rope chamber, connecting box, rope, right rope sheave, rope chamber, connecting box, humidity inductor, the upper right side of left rope sheave is equipped with right rope sheave, left rope sheave is connected by the rope with right rope sheave, right rope sheave fixed mounting is on right rope sheave shaft, right rope sheave front and back end rotate respectively and install in the end wall around the rope chamber, rope chamber below is equipped with the connecting box, the rope lower extreme is through right rope sheave and left rope sheave fixed connection on tensile magnetism board, connecting box left end fixed mounting has the humidity.
Further, the oxygen control assembly comprises a sensing box, the sensing box is located below the carbon dioxide control cavity, a left stretching magnetic plate is arranged in the sensing box, a carbon dioxide sensor is fixedly mounted at the right end of the sensing box, a right sensing magnet which is attracted to the left stretching magnetic plate is arranged in the carbon dioxide sensor, a left lifting rod and a right lifting rod are fixedly mounted on the left stretching magnetic plate, an oxygen compression cavity is arranged above the sensing box, an upper push plate is fixedly mounted above the left lifting rod, the left end and the right end of the upper push plate are abutted to the left wall end and the right wall end of the oxygen compression cavity, oxygen is arranged above the upper push plate, an air outlet pipe is arranged above the oxygen compression cavity and communicated with the culture cavity, a left blocking plate is arranged in the air outlet pipe, the right end of the left blocking plate is fixedly mounted on the inner wall of the air outlet pipe, and a left spring is arranged on the left side of the, and the right side of the left spring is fixedly arranged in the right wall end of the air outlet pipe.
Furthermore, a left rack is fixedly arranged above the right lifting rod, a gear is arranged on the right side of the left rack, the gear is fixedly arranged on a gear shaft, the front end and the rear end of the gear shaft are respectively and rotatably arranged in the front wall end and the rear wall end of the carbon dioxide control cavity, a right rack is arranged on the right side of the gear, a right mandril is arranged above the right rack, a right compression plate is fixedly arranged above the right mandril and is arranged in the cavity in a sliding way, the left end and the right end of the right compression plate are propped in the left wall end and the right wall end with the cavity, an air inlet pipe is arranged above the cavity, the air inlet pipe is communicated with the humidity control cavity, a right blocking plate is arranged in the air outlet pipe, the right end of the right blocking plate is fixedly arranged on the inner wall of the air inlet pipe, the right side hinders board left side and is equipped with right spring, right spring right side fixed mounting is in the right wall end of intake pipe.
The invention has the beneficial effects that: the device has the advantages of simple structure and convenient operation, the equipment provided by the invention has the advantages of high automation degree, close connection among equipment structures, strong integrity, higher application range and practicability, and no influence of external factors when the embryos are cultured, and the embryos are cultured in a totally-enclosed manner until the culture is completed.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic top view of a component 24 of the present invention;
FIG. 3 is a schematic view of A-A in FIG. 1 according to an embodiment of the present invention;
FIG. 4 is an enlarged schematic view of the structure at B in FIG. 1 according to an embodiment of the present invention;
FIG. 5 is an enlarged schematic view of the structure at C in FIG. 1 according to an embodiment of the present invention;
fig. 6 is an enlarged schematic view of the structure at D in fig. 1 according to the embodiment of the present invention.
Detailed Description
The invention will now be described in detail with reference to fig. 1-6, wherein for ease of description the orientations described hereinafter are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.
With reference to fig. 1-6, the culture apparatus for embryo cultivation includes a case 28, a lower pressing cavity 38 is disposed at the upper left of the case 28, an extrusion cavity 74 is disposed at the lower right of the lower pressing cavity 38, a bevel gear cavity 36 is disposed below the lower pressing cavity 38, a transmission cavity 91 is disposed below the bevel gear cavity 36, a cultivation cavity 93 is disposed above the transmission cavity 91, a pressing plate 73 is disposed in the extrusion cavity 74, the left and right ends of the pressing plate 73 are supported in the left and right end walls of the extrusion cavity 74, a nutrient solution 71 is disposed below the pressing plate 73, a spraying sieve plate 72 is disposed below the nutrient solution 71, the spraying sieve plate 72 is fixedly mounted on the lower end wall of the extrusion cavity 74, the right side of the nutrient solution 71 in the extrusion cavity 74 is communicated with a nutrient solution storage cavity 47, a nutrient solution is disposed in the nutrient solution storage cavity 47, the nutrient solution storage cavity 47 is disposed at the right of the lower pressing cavity 38, nutrient solution storage chamber 47 top is equipped with filling opening 79, pressure strip 73 top fixed mounting has depression bar 78 down, depression bar 78 upper end fixedly connected with connecting rod 77 down, connecting rod 77 left side fixed mounting has fixed sliding block 45, the fixed sliding block 45 fixed mounting of connecting rod 77 left end is in carousel sliding tray 43, carousel sliding tray 43 is located carousel 46, carousel 46 fixed mounting is on axis of rotation 42, the axis of rotation 42 front and back end rotates respectively to be installed in the preceding rear end wall of depression chamber 38 down, the carousel 46 rear side is equipped with right turn gear 44, right turn gear 44 fixed mounting is at axis of rotation 42, right turn gear 44 left side gear engagement is connected with left turn gear 39, left turn gear 39 fixed mounting is on transmission shaft 40, transmission shaft 40 front and back end rotates respectively to be installed in the preceding rear end wall of depression chamber 38 down, the left rotating gear 39 is provided with an upper belt wheel 41 on the front side, the upper belt wheel 41 is fixedly mounted on the transmission shaft 40, a culture assembly is arranged below the upper belt wheel 41, the right side of the pressing cavity 38 is provided with a humidity control cavity 90, a cavity 63 is arranged below the humidity control cavity 90, a humidity control assembly is arranged in the humidity control cavity 90, a carbon dioxide control cavity 68 is arranged below the humidity control cavity 90, and an oxygen control assembly is arranged in the carbon dioxide control cavity 68.
Beneficially, the culture assembly comprises a lower belt gear 35, the lower belt gear 35 and an upper belt pulley 41 are connected by a belt 37, the lower belt gear 35 is fixedly installed on a belt shaft 33, the front end and the rear end of the belt shaft 33 are respectively installed in the front end wall and the rear end wall of the bevel gear cavity 36 in a rotating manner, the rear side of the lower belt gear 35 is provided with a left transmission bevel gear 34, the left transmission bevel gear 34 is fixedly installed on the belt shaft 33, the front side of the left transmission bevel gear 34 is provided with a right transmission bevel gear 32, the right transmission bevel gear 32 is in gear meshing connection with the left transmission bevel gear 34, the right transmission bevel gear 32 is fixedly installed on a bevel gear shaft 31, the right end of the bevel gear shaft 31 is installed in the right end wall of the bevel gear cavity 36 in a rotating manner, the right side of the right transmission bevel gear 32 is provided with an upper bevel, the lower bevel gear 29 is arranged below the upper bevel gear 30, the lower bevel gear 29 is fixedly arranged on a gear transmission shaft 26, the gear transmission shaft 26 is rotatably arranged between the bevel gear cavity 36 and the transmission cavity 91, the lower end of the gear transmission shaft 26 is in power connection with a motor 27 in the bottom wall of the transmission cavity 91, a sector wheel 49 is arranged in the transmission cavity 91, the sector wheel 49 is fixedly arranged on the gear transmission shaft 26, a rotating rod 50 is fixedly connected below the sector wheel 49, a boss 92 is arranged above the right of the rotating rod 50, an intermittent wheel 53 is arranged on the right side of the sector wheel 49, an intermittent shaft 25 is rotatably arranged between the carbon dioxide control cavity 68 and the transmission cavity 91, the lower end of the intermittent shaft 25 extends into the transmission cavity 91 and is fixedly connected with the intermittent wheel 53, four clamping grooves 51 are arranged in the intermittent wheel 53, a supporting disc 24 is arranged above the intermittent wheel 53, the upper end of the intermittent shaft 25 extends into the culture cavity 93 and is fixedly connected with the supporting disc 24, four placing grooves 23 are formed in the supporting disc 24, and four culture dishes 22 are arranged on the four placing grooves 23.
Beneficially, the humidity control assembly comprises a water tank 8, clear water 5 is arranged in the water tank 8, the water tank 8 is fixedly mounted on the lower wall end of the humidity control chamber 90, a cover plate 1 is arranged above the water tank 8, two left fixing rods 3 are arranged in front of and behind the left side of the cover plate 1, the lower ends of the two left fixing rods 3 are fixedly mounted on the outer box body at the lower end of the water tank 8, a fixing spring 2 is arranged on the left side of the left fixing rod 3, the left side of the fixing spring 2 is fixedly mounted on the water tank 8, two right fixing rods 6 are arranged in front of and behind the right side of the cover plate 1, the lower ends of the two right fixing rods 6 are fixedly mounted on the outer box body at the lower end of the water tank 8, a connecting rod 9 is fixedly connected between the two right fixing rods 6, a rope 17 is fixedly connected on the, the left rope wheel 11 is fixedly installed on the left rope wheel shaft 10, the front end and the rear end of the left rope wheel shaft 10 are respectively rotatably installed in the front end wall and the rear end wall of the rope cavity 14, a right rope wheel 12 is arranged on the upper right side of the left rope wheel 11, the left rope wheel 11 is connected with the right rope wheel 12 through a rope 17, the right rope wheel 12 is fixedly installed on the right rope wheel shaft 13, the front end and the rear end of the right rope wheel shaft 13 are respectively rotatably installed in the front end wall and the rear end wall of the rope cavity 14, a connecting box 18 is arranged below the rope cavity 14, the lower end of the rope 17 is fixedly connected onto a stretching magnetic plate 19 through the right rope wheel 12 and the left rope wheel 11, a humidity sensor 94 is fixedly installed at the left end of the connecting box 18, and a sensing.
Beneficially, the oxygen control assembly comprises a sensing box 99, the sensing box 99 is located below the carbon dioxide control cavity 68, a left stretching magnetic plate 20 is arranged in the sensing box 99, a carbon dioxide sensor 21 is fixedly mounted at the right end of the sensing box 99, a right sensing magnet attracted to the left stretching magnetic plate 20 is arranged in the carbon dioxide sensor 21, a left lifting rod 59 and a right lifting rod 76 are fixedly mounted on the left stretching magnetic plate 20, an oxygen compression cavity 58 is arranged above the sensing box 99, an upper push plate 60 is fixedly mounted above the left lifting rod 59, the left and right ends of the upper push plate 60 are supported in the left and right wall ends of the oxygen compression cavity 58, oxygen 61 is arranged above the upper push plate 60, an air outlet pipe 62 is arranged above the oxygen compression cavity 58, the air outlet pipe 62 is communicated with the culture cavity 93, a left blocking plate 56 is arranged in the air outlet pipe 62, the right end of the left blocking plate 56 is fixedly arranged on the inner wall of the air outlet pipe 62, a left spring 57 is arranged on the left side of the left blocking plate 56, and the right side of the left spring 57 is fixedly arranged in the right wall end of the air outlet pipe 62.
Beneficially, a left rack 75 is fixedly mounted above the right lifting rod 76, a gear 16 is arranged on the right side of the left rack 75, the gear 16 is fixedly mounted on a gear shaft 15, the front end and the rear end of the gear shaft 15 are respectively rotatably mounted in the front wall end and the rear wall end of the carbon dioxide control chamber 68, a right rack 66 is arranged on the right side of the gear 16, a right ejector rod 65 is arranged above the right rack 66, a right compression plate 64 is fixedly mounted above the right ejector rod 65, the right compression plate 64 is slidably mounted in the cavity 63, the left end and the right end of the right compression plate 64 are abutted against the left wall end and the right wall end of the cavity 63, an air inlet pipe 100 is arranged above the cavity 63, the air inlet pipe 100 is communicated with the humidity control chamber 90, a right blocking plate 70 is arranged in the air outlet pipe 62, the right blocking plate 70 is fixedly mounted on the inner wall of the, the right side of the right spring 69 is fixedly installed in the right wall end of the intake duct 100.
Sequence of mechanical actions of the whole device:
1: put into culture dish 22 to the embryo that needs were cultivateed, then put into standing groove 23 with culture dish 22 in, placed back starter motor 27, thereby motor 27 rotates and drives gear drive shaft 26 and rotates, thereby gear drive shaft 26 rotates and drives fan-shaped wheel 49, fan-shaped wheel 49 rotates and drives dwang 50 and rotate, when dwang 50 every turn quarter round, thereby boss 92 on the dwang 50 can block into draw-in groove 51 and drive intermittent type wheel 53 and rotate the quarter round, then fan-shaped wheel 49 can rotate the quarter round with intermittent type wheel 53 relatively, forms an intermittent type and rotates, intermittent type wheel 53 can drive intermittent type axle 25 when rotating, intermittent type axle 25 rotates and can drive support disc 24 and rotate, thereby support disc 24 rotates and drive culture dish 22 and rotate the quarter round and turn to spraying sieve plate 72 below.
2: when the transmission shaft 26 rotates, the lower bevel gear 29 is driven to rotate, when the lower bevel gear 29 rotates, the upper bevel gear 30 is driven to rotate, the upper bevel gear 30 rotates to drive the bevel gear shaft 31 to rotate, the bevel gear shaft 31 rotates to drive the right transmission bevel gear 32 to rotate, the right transmission bevel gear 32 rotates to drive the left transmission bevel gear 34 to rotate, the left transmission bevel gear 34 rotates to drive the belt shaft 33 to rotate, the belt shaft 33 rotates to drive the lower belt gear 35 to rotate, the lower belt gear 35 rotates to drive the upper belt pulley 41 to rotate through the belt 37, the upper belt pulley 41 rotates to drive the transmission shaft 40 to rotate, the transmission shaft 40 rotates to drive the left rotation gear 39 to rotate, the left rotation gear 39 rotates to drive the right rotation gear 44 to rotate, the right rotation gear 44 rotates to drive the rotation shaft 42 to rotate, and the rotation shaft 42 rotates to drive the turntable 46 to rotate, the rotating disc 46 rotates to drive the fixed sliding block 45 of the rotating disc sliding groove 43 to rotate, the fixed sliding block 45 rotates to drive the connecting rod 77 to move up and down so as to drive the lower pressing rod 78 to move up and down in an interference mode, the lower pressing rod 78 moves up and down in a reciprocating mode so as to drive the pressing plate 73 to move up and down in the extrusion cavity 74, and the pressing plate 73 moves up and down in the extrusion cavity 74 so as to compress the nutrient solution 71 and evenly spray the nutrient solution onto embryos in the culture dish 22 through the spraying sieve plate 72.
3: after four culture dishes 22 of support disc 24 all sprayed nutrient solution 71, motor 27 is closed, wait for the embryo in culture dish 22 to cultivate, when cultivateing the embryo the content of the carbon dioxide of culture chamber 93 rises, contain right induction magnet in carbon dioxide inductor 21, can make left side stretch magnetic sheet 20 can corresponding the rising because of suction when right induction magnet rises, thereby make left side stretch magnetic sheet 20 rise and make left lifting rod 59 rise, thereby left lifting rod 59 rises and make push-up board 60 rise, thereby push-up board 60 rises and makes the oxygen that promotes in the oxygen compression chamber 58 extrude to left hindrance board 56 from outlet duct 62, left hindrance board 56 receives pressure and can compress tightly left spring 57 messenger oxygen culture chamber 93.
4: meanwhile, when the left stretching magnetic plate 20 is lifted, the right lifting rod 76 is lifted, the left rack 75 is lifted to drive the gear 16 to rotate, the gear 16 is rotated to drive the right rack 66 to descend, the right rack 66 is descended to drive the right compression plate 64 to descend, the air pressure of the cavity 63 and the air pressure of the humidity control cavity 90 are different, and the right blocking plate 70 is pressed against the right spring 69, so that the right blocking plate 70 is pressed against the right spring 69, and the left compression plate 64 is pressed against the left
Carbon dioxide in the humidity control chamber 90 enters the cavity 63 to reduce the carbon dioxide content in the culture chamber 93.
5: when the culture cavity 93 is too dry, the humidity sensor 94 contains the sensing magnet 95, when the humidity is too low, the sensing magnet 95 in the humidity sensor 94 can descend, when the sensing magnet 95 descends, the stretching magnetic plate 19 can correspondingly descend due to suction force, so that the stretching magnetic plate 19 descends to enable the rope 17 to descend, the rope 17 descends to enable the rope 17 to be pulled to the connecting rod 9 through the right rope pulley 12 and the left rope pulley 11 to move rightwards, the connecting rod 9 moves rightwards to enable the right fixing rod 6 to be pulled to rotate around the rotating shafts of the water tank 8 and the connecting rod 9, so that the cover plate 1 is opened, the clean water 5 in the water tank 8 is exposed in the humidity control cavity 90, the humidity control cavity 90 is communicated with the culture cavity 93, so that the humidity of the culture cavity 93 is increased, when nutrient solution needs to be injected again after a period of time, the motor 27 is opened again, and the culture is repeated for.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims (5)

1. The utility model provides a culture apparatus for embryo culture, includes the box, its characterized in that: a lower pressing cavity is arranged at the upper left of the box body, an extrusion cavity is arranged at the lower right of the lower pressing cavity, a bevel gear cavity is arranged below the lower pressing cavity, a transmission cavity is arranged below the bevel gear cavity, a culture cavity is arranged above the transmission cavity, a pressing plate is arranged in the extrusion cavity, the left end and the right end of the pressing plate are abutted into the left end wall and the right end wall of the extrusion cavity, nutrient solution is arranged below the pressing plate, a spray sieve plate is arranged below the nutrient solution, and the spray sieve plate is fixedly arranged on the lower end wall of the extrusion cavity; the right side of the nutrient solution in the extrusion cavity is communicated with a nutrient solution storage cavity, the nutrient solution storage cavity is internally provided with nutrient solution, the nutrient solution storage cavity is arranged on the right side of the lower pressure cavity, an injection hole is formed above the nutrient solution storage cavity, a lower pressure rod is fixedly arranged above the pressure plate, the upper end of the lower pressure rod is fixedly connected with a connecting rod, the left side of the connecting rod is fixedly provided with a fixed sliding block, the fixed sliding block at the left end of the connecting rod is fixedly arranged in a rotary table sliding groove, the rotary table sliding groove is positioned in a rotary table, the rotary table is fixedly arranged on a rotary shaft, and the front end and the rear end of the rotary shaft are respectively and rotatably arranged in; the carousel rear side is equipped with right turn gear, right turn gear fixed mounting is in the axis of rotation, right turn gear left side gear engagement is connected with the left-hand gear, left-hand gear fixed mounting is on the transmission shaft, the transmission shaft front and back end rotates respectively to be installed in the end wall around the chamber pushes down, left-hand gear front side is equipped with belt pulley, belt pulley fixed mounting is on the transmission shaft on going up, it is equipped with cultivates the subassembly to go up the belt pulley below, it is equipped with humidity control chamber to push down the chamber right side, humidity control chamber below is equipped with the cavity, the humidity control intracavity is equipped with humidity control assembly, humidity control chamber below is equipped with carbon dioxide control chamber, carbon dioxide control intracavity is equipped with oxygen control assembly.
2. A culture device for embryo culture according to claim 1, wherein: the culture assembly comprises a lower belt gear, the lower belt gear is connected with an upper belt wheel through a belt, the lower belt gear is fixedly arranged on a belt shaft, the front end and the rear end of the belt shaft are respectively rotatably arranged in the front end wall and the rear end wall of the helical gear cavity, a left transmission helical gear is arranged on the rear side of the lower belt gear, the left transmission helical gear is fixedly arranged on the belt shaft, a right transmission helical gear is arranged on the front side of the left transmission helical gear, the right transmission helical gear is meshed with the left transmission helical gear, the right transmission helical gear is fixedly arranged on a helical gear shaft, the right end of the helical gear shaft is rotatably arranged in the right wall end of the helical gear cavity, an upper helical gear is arranged on the right side of the right transmission helical gear, the upper helical gear is fixedly arranged on the helical gear shaft, a lower helical gear is arranged below the, the gear transmission shaft is rotatably arranged between the bevel gear cavity and the transmission cavity, the lower end of the gear transmission shaft is in power connection with a motor fixedly arranged in the bottom wall of the transmission cavity, a sector wheel is arranged in the transmission cavity, the sector wheel is fixedly arranged on the gear transmission shaft, a rotating rod is fixedly connected below the sector wheel, a boss is arranged at the upper right part of the rotating rod, an intermittent wheel is arranged at the right side of the fan-shaped wheel, an intermittent shaft is rotatably arranged between the carbon dioxide control cavity and the transmission cavity, the lower end of the intermittent shaft extends into the transmission cavity and is fixedly connected with the intermittent wheel, four clamping grooves are arranged in the intermittent wheel, a supporting disc is arranged above the intermittent wheel, the upper end of the intermittent shaft extends into the culture cavity and is fixedly connected with the supporting disc, four placing grooves are formed in the supporting disc, and four culture dishes are arranged on the four placing grooves.
3. A culture device for embryo culture according to claim 1, wherein: the humidity control assembly comprises a water tank, clear water is arranged in the water tank, the water tank is fixedly arranged on the lower wall end of the humidity control cavity, a cover plate is arranged above the water tank, two left fixing rods are arranged on the front and back of the left side of the cover plate, the lower ends of the two left fixing rods are fixedly arranged on the outer lower end box body of the water tank, a fixing spring is arranged on the left side of the left fixing rod, the left side of the fixing spring is fixedly arranged on the water tank, two right fixing rods are arranged on the front and back of the right side of the cover plate, the lower ends of the two right fixing rods are fixedly arranged on the outer lower end box body of the water tank, a connecting rod is fixedly connected between the two right fixing rods, a rope is fixedly connected on the right side of the connecting rod, a rope cavity is arranged on the right side of the humidity control cavity, a, the utility model discloses a tensile magnetism board, including left rope sheave, right rope sheave, rope chamber, connecting box, rope, right rope sheave, rope chamber, connecting box, humidity inductor, the upper right side of left rope sheave is equipped with right rope sheave, left rope sheave is connected by the rope with right rope sheave, right rope sheave fixed mounting is on right rope sheave shaft, right rope sheave front and back end rotate respectively and install in the end wall around the rope chamber, rope chamber below is equipped with the connecting box, the rope lower extreme is through right rope sheave and left rope sheave fixed connection on tensile magnetism board, connecting box left end fixed mounting has the humidity.
4. A culture device for embryo culture according to claim 2, wherein: the oxygen control assembly comprises a sensing box, the sensing box is positioned below a carbon dioxide control cavity, a left stretching magnetic plate is arranged in the sensing box, a carbon dioxide sensor is fixedly arranged at the right end of the sensing box, a right sensing magnet which is attracted with the left stretching magnetic plate is arranged in the carbon dioxide sensor, a left lifting rod and a right lifting rod are fixedly arranged on the left stretching magnetic plate, an oxygen compression cavity is arranged above the sensing box, an upper push plate is fixedly arranged above the left lifting rod, the left end and the right end of the upper push plate are supported in the left wall end and the right wall end of the oxygen compression cavity, oxygen is arranged above the upper push plate, an air outlet pipe is arranged above the oxygen compression cavity, the air outlet pipe is communicated with a culture cavity, a left blocking plate is arranged in the air outlet pipe, the right end of the left blocking plate is fixedly arranged on the inner wall of the air outlet pipe, and a left spring is, and the right side of the left spring is fixedly arranged in the right wall end of the air outlet pipe.
5. A culture device for embryo culture according to claim 3, wherein: right lifting rod top fixed mounting has left rack, left side rack right side is equipped with the gear, gear fixed mounting is on the gear shaft, the gear shaft front and back end rotates respectively to be installed in the front and back wall end in carbon dioxide control chamber, the gear right side is equipped with right rack, right rack top is equipped with right ejector pin, right ejector pin top fixed mounting has right compression plate, right compression plate slidable mounting in the cavity, right compression plate left and right ends are supported in there being the cavity wall end about, the cavity top is equipped with the intake pipe, intake pipe and humidity control chamber intercommunication, be equipped with a right hindrance board in the outlet duct, right hindrance board right-hand member fixed mounting is at the inner wall of intake pipe, right side hindrance board left side is equipped with right spring, right spring right side fixed mounting is in the right wall end of intake pipe.
CN201911176087.7A 2019-11-26 2019-11-26 A culture apparatus for embryo culture Withdrawn CN110724638A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911176087.7A CN110724638A (en) 2019-11-26 2019-11-26 A culture apparatus for embryo culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911176087.7A CN110724638A (en) 2019-11-26 2019-11-26 A culture apparatus for embryo culture

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CN110724638A true CN110724638A (en) 2020-01-24

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Application Number Title Priority Date Filing Date
CN201911176087.7A Withdrawn CN110724638A (en) 2019-11-26 2019-11-26 A culture apparatus for embryo culture

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Country Link
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112048442A (en) * 2020-10-13 2020-12-08 广州科为生物科技有限公司 Biotechnology analysis consultation equipment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112048442A (en) * 2020-10-13 2020-12-08 广州科为生物科技有限公司 Biotechnology analysis consultation equipment
CN112048442B (en) * 2020-10-13 2021-07-23 广州科为生物科技有限公司 Biotechnology analysis consultation equipment

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