CN107058099B

CN107058099B - Embryo culture and transportation device

Info

Publication number: CN107058099B
Application number: CN201710273934.6A
Authority: CN
Inventors: 刁云飞; 李晓霞; 许保增; 王士勇; 张宇飞; 常彤; 李丹丽
Original assignee: Institute Special Animal and Plant Sciences CAAS
Current assignee: Institute Special Animal and Plant Sciences CAAS
Priority date: 2017-04-24
Filing date: 2017-04-24
Publication date: 2023-06-27
Anticipated expiration: 2037-04-24
Also published as: CN107058099A

Abstract

The invention provides an embryo culture and transportation device, which belongs to the field of cell transportation and comprises an incubator, an ultrasonic generator, a heating element, a gas circuit one-way valve and a sensor for sensing carbon dioxide concentration, temperature and humidity. The incubator comprises an incubation cavity, and the inductor is fixedly arranged in the incubation cavity; the outer side of one end of the incubator is provided with a containing part, the containing part comprises a system containing cavity and a water tank, and the regulating system is contained in the system containing cavity; the embryo culturing and transporting device also comprises an adjusting system, the adjusting system is electrically connected with the ultrasonic generator, the adjusting system is electrically connected with the heating element, and the adjusting system is connected with the air passage one-way valve. Through the embryo culture and transportation device, temperature regulation, humidity regulation and carbon dioxide concentration regulation can be simultaneously provided in the embryo or oocyte transportation process, so that the ideal environment for the survival of the embryo or oocyte is achieved in the culture cavity.

Description

Embryo culture and transportation device

Technical Field

The invention relates to the field of cell transportation, in particular to an embryo culture and transportation device.

Background

Embryo engineering technology is a research field which is developed rapidly in recent years, has important effects on genetic improvement of livestock and preservation of rare wild animal resources, and also has important effects on treatment of infertility of human beings. However, since animal breeding bases are often remote from the laboratory, the test samples need to be transported back to the laboratory over a longer period of time, which often results in cell contamination and reduced cell viability. Meanwhile, since the cells are transported in order to ensure the stability of transportation, the test sample is usually placed in a fixed state, so that it is difficult to simultaneously satisfy the culture conditions required by the cells in the culture process.

Moreover, the existing embryo or oocyte transport devices can only perform the function of heat preservation, and cannot well simulate the ideal environment suitable for embryo survival.

Disclosure of Invention

The invention provides an embryo culture and transportation device, which aims to solve the problems of the embryo culture and transportation device in the prior art.

The invention is realized in the following way:

an embryo culturing and transporting device comprises an incubator, an ultrasonic generator, a heating element, a gas path one-way valve and an inductor for inducing carbon dioxide concentration, temperature and humidity.

The incubator comprises a culture cavity, and the inductor is fixedly arranged in the culture cavity;

the outer side of one end of the incubator is provided with a containing part, the containing part comprises a system containing cavity and a water tank, and the regulating system is contained in the system containing cavity;

the embryo culture and transportation device further comprises an adjusting system, wherein the adjusting system is electrically connected with the ultrasonic generator, the adjusting system is electrically connected with the heating element, and the adjusting system is connected with the air passage one-way valve.

In a preferred embodiment of the present invention, the ultrasonic generator is disposed in the water tank, and the heating element is disposed on a surface of the culture chamber close to the accommodating portion.

In a preferred embodiment of the present invention, the embryo culturing and transporting device further includes a premix gas device, the premix gas device includes a premix gas reservoir and a premix gas adjusting induction valve, and a gas outlet of the premix gas adjusting induction valve is connected with a gas inlet of the gas path check valve.

In a preferred embodiment of the invention, the embryo culturing and transporting device further comprises an air passage, wherein the air passage is communicated with the water tank and the side wall of the culturing cavity, and the air outlet of the air passage one-way valve is communicated with the air passage.

In a preferred embodiment of the present invention, the embryo culturing and transporting device further includes a transporting rack, wherein a slide way is disposed at one end of the culturing cavity near the opening, the transporting rack includes a fixing rod and a rack body for placing a straw or a centrifugal test tube, the fixing rod is connected with the rack body through a flexible wire, and the fixing rod can be fixed in the culturing cavity through the slide way.

In a preferred embodiment of the present invention, the frame body includes a bottom layer and a fixing layer, the bottom layer is fixedly installed on a surface of the fixing layer, which is far away from the fixing rod, and the bottom layer is provided with a bracket.

In a preferred embodiment of the invention, the fixing layer is provided with a through hole matched with the bracket.

In a preferred embodiment of the invention, a buffer rod is further arranged in the culture cavity, the buffer rod comprises a first rod body and a second rod body which is sleeved in the first rod body in a sealing way, one end of the buffer rod is fixedly connected with the inner wall of the culture cavity, and the other end of the buffer rod is abutted against the frame body.

In a preferred embodiment of the present invention, a plurality of protruding columns are disposed on a side of the buffer rod, which is close to the opening, and the protruding columns are located on the same plane.

An embryo culturing and transporting device comprises a handle, an incubator, an ultrasonic generator, a heating element, an air passage one-way valve and an inductor for inducing carbon dioxide concentration, temperature and humidity.

the embryo culture and transportation device further comprises an adjusting system, wherein the adjusting system is electrically connected with the ultrasonic generator, the adjusting system is electrically connected with the heating piece, and the adjusting system is connected with the air passage one-way valve;

the handle is arranged on the outer side of the incubator.

The beneficial effects of the invention are as follows: the embryo culturing and transporting device provided by the invention can simultaneously provide temperature regulation, humidity regulation and carbon dioxide concentration regulation in the embryo or oocyte transporting process, so that the ideal environment for the survival of the embryo or oocyte is achieved in the culturing cavity. Embryo culture and conveyer can select installation transport frame to carry out embryo culture under the transportation state according to actual need through detachable transportation frame setting, can provide the buffering for the support body through the effect of patchcord and buffering pole under this state, avoids embryo damage in the transportation. When the embryo culturing rack is not required to be transported, the transportation rack is taken out and replaced by the plate bracket, and the embryo culturing rack is directly placed in a plate for static culturing of embryos. The device not only can be used for embryo transportation, but also can be used for transporting active semen after the adjusting system is adjusted.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a cross-sectional view of an embryo culture and transport device according to an embodiment of the present invention;

FIG. 2 is a cross-sectional view of an embryo culture and transport device according to an embodiment of the present invention in a state in which a centrifuge tube is placed;

FIG. 3 is a schematic diagram of an embryo culturing and transporting device according to an embodiment of the present invention;

FIG. 4 is a cross-sectional view taken along the direction IV-IV in FIG. 1;

FIG. 5 is an enlarged partial view of area A of FIG. 1;

FIG. 6 is a schematic view of a plate holder according to an embodiment of the present invention;

fig. 7 is a schematic structural diagram of an embryo culturing and transporting device according to a second embodiment of the present invention.

Icon: 001-embryo culture and transport device; 010-incubator; 020-an ultrasonic generator; 030-heating net; 040-gas circuit check valve; 013-display; 100-culture chamber; 200-an inductor; 300-an accommodating part; 310-a system housing cavity; 330-a water tank; 311-a conditioning system; 011-extension; 331-airway; 410-a premix gas bladder; 430-a premix gas regulation induction valve; 500-transporting frames; 110-opening; 111-slide ways; 510-fixing the rod; 530-a frame; 550-flexible wires; 570-centrifuging the tube; 531-bottom layer; 533-fixed layer; 5331-a via; 5311-brackets; 534-elastic clip; 5341-a first clamp block; 5343-a second clamp block; 5345-a clamping spring; 590-a buffer rod; 591-a first stick body; 593-a second stick body; 580-posts; 600-plate rack; 610-a bracket through hole; 050-handle.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.

In the description of the present invention, it should be understood that the terms "orientation" or "positional relationship" are based on the orientation or positional relationship shown in the drawings, and are merely for convenience of description and to simplify the description, rather than to indicate or imply that the apparatus or elements referred to must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as limiting the invention.

In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present invention, unless expressly stated or limited otherwise, a first feature may include first and second features directly contacting each other, either above or below a second feature, or through additional features contacting each other, rather than directly contacting each other. Moreover, the first feature being above, over, and on the second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being below, beneath, and beneath the second feature includes the first feature being directly below and obliquely below the second feature, or simply indicates that the first feature is less level than the second feature.

Example 1

The present embodiment provides an embryo culturing and transporting device 001, referring to fig. 1, the embryo culturing and transporting device 001 includes an incubator 010, an ultrasonic generator 020, a heating element and an air passage check valve 040.

The incubator 010 includes a culture chamber 100 inside, and a sensor 200 is fixedly installed on one side wall of the culture chamber 100, and the sensor 200 can sense the concentration of carbon dioxide, humidity and temperature in the culture chamber 100.

The accommodation part 300 is provided outside the bottom surface of the incubator 010, and the accommodation part 300 and the culture chamber 100 are isolated from each other by the bottom of the incubator 010. The accommodating portion 300 includes a system accommodating cavity 310 and a water tank 330, wherein an adjusting system 311 is accommodated in the system accommodating portion 300, the adjusting system 311 is electrically connected with the sensor 200, and the adjusting system 311 can receive a carbon dioxide concentration signal, a temperature signal and a humidity signal transmitted from the sensor 200, and perform analysis processing, so as to transmit corresponding adjusting signals to corresponding devices. And a display 013 for displaying the carbon dioxide concentration, temperature and humidity in the incubator 010 is provided outside the incubator 010. The display 013 is a touch screen, and the carbon dioxide concentration, the temperature and the humidity can be manually adjusted through virtual keys on the touch screen.

The water tank 330 is arranged at one side of the accommodating part 300 far away from the inductor 200, and an extension part 011 is further arranged at one side of the incubator 010 far away from the inductor 200, and the extension part 011 is hollow to form an air passage 331 to communicate the culture cavity 100 with the water tank 330. Through the air passage 331, water in the water tank 330 can enter the incubator 010 to adjust the humidity in the incubator 010.

The ultrasonic generator 020 is disposed in the water tank 330, and the ultrasonic generator 020 is electrically connected to the adjustment system 311, and the switch of the ultrasonic generator 020 is adjusted by an adjustment signal emitted from the adjustment system 311. The ultrasonic wave emitted from the ultrasonic generator 020 atomizes the water in the water tank 330 so that the mist enters the culture chamber 100 to adjust the humidity in the culture chamber 100.

In this embodiment, the heating element is a heating net 030, the heating net 030 is tiled at the bottom of the culture cavity 100, and the current switch of the heating net 030 is controlled by the adjusting system 311 to control the temperature in the culture cavity 100.

The air passage check valve 040 is arranged on one side of the extension portion 011 away from the culture cavity 100, the outlet of the air passage check valve 040 faces the air passage 331, and the inlet of the air passage check valve 040 is connected with the premix gas device. The premix apparatus includes a premix gas reservoir, in this embodiment a premix gas bladder 410, and a premix gas adjusting induction valve 430, wherein the premix gas bladder 410 is filled with a premix gas having a proper carbon dioxide concentration for embryo development. The premixed gas adjusting induction valve 430 is arranged in the premixed gas gasbag 410, the concentration of carbon dioxide in the premixed gas gasbag 410 and the pressure of the premixed gas in the premixed gas gasbag 410 can be sensed through the premixed gas adjusting induction valve 430, a control switch of the gas path one-way valve 040 is connected with the adjusting system 311, and the gas path one-way valve 040 can be controlled to be closed or opened through the adjusting system 311 so as to control the concentration of carbon dioxide in the culture cavity 100.

Referring to fig. 2, 3 and 4, the embryo culturing and transporting device 001 further comprises a transporting frame 500, an opening 110 is provided at one end of the culturing chamber 100 far away from the accommodating portion 300, four sliding ways 111 facing the bottom are provided on the opening 110, the transporting frame 500 comprises two fixing rods 510 and a frame 530, two fixing rods 510 are provided, and two ends of the fixing rod 510 can slide into the culturing chamber 100 through the sliding ways 111 and abut against the end positions of the sliding ways 111. The fixing lever 510 and the frame 530 are connected by a flexible wire 550 to buffer the rigid collision of the centrifugal test tube 570 during transportation.

In the present invention, the flexible wire 550 may be wrapped with an elastic member or a shock-absorbing wire made of a material having a certain elasticity such as rubber, so that the fixing rod 510 and the frame 530 can be shock-absorbed in both directions, and the embryo culturing and transporting device 001 has a buffering effect when vibrating up and down.

The frame 530 includes a bottom layer 531 and a fixed layer 533, the bottom layer 531 and the fixed layer 533 are fixedly connected by a connecting member, and the bottom layer 531 is disposed at one end of the fixed layer 533 away from the fixed rod 510. A through hole 5331 is provided in the fixed layer 533, and the centrifugation test tube 570 can be abutted on the bottom layer 531 through the through hole 5331. The position corresponding to the through hole 5331 on the bottom layer 531 is provided with a bracket 5311, when the centrifugation test tube 570 is placed through the through hole 5331 and is abutted on the bottom layer 531, the bottom of the centrifugation test tube 570 cooperates with the bracket 5311 for the placement of the centrifugation test tube 570 is more stable.

The elastic clamp 534 is further provided at both sides of the through hole 5331, the elastic clamp 534 includes a first clamp block 5341, a second clamp block 5343 and two clamp springs 5345, the first clamp block 5341 and the second clamp block 5343 are slidably disposed at both sides of the through hole 5331, when the first clamp block 5341 and the second clamp block 5343 are far away from the through hole 5331, the through hole 5331 is completely opened, and the two clamp springs 5345 connect the first clamp block 5341 and the second clamp block 5343 so that the first clamp block 5341 and the second clamp block 5343 can be automatically close to each other by elastic force. Not only can the stability of the centrifugal test tube 570 be enhanced by the elastic clamp 534, but also the distance between the first clamp block 5341 and the second clamp block 5343 can be shortened to clamp the straw. Can be clamped between the first clamping block 5341 and the second clamping block 5343 towards different directions according to the actual placing requirement of the straws. And the elastic clamp 534 may be hidden in the fixed layer 533 by providing a groove on the fixed layer 533.

Referring to fig. 1 and 5, in this embodiment, the culture cavity 100 is a square cavity, three buffer rods 590 are disposed on each side wall of the square cavity, the buffer rods 590 are perpendicular to the side walls, the buffer rods 590 include a first rod body 591 and a second rod body 593, the first rod body 591 is partially sealed and sleeved in the second rod body 593, an air cavity is formed in the second rod body 593, when the first rod body 591 presses against the second rod body 593, a buffer effect can be formed by air pressure of the air cavity, and when the pressure disappears, air pressure in the air cavity can return to the original position. In this embodiment, an end of the second rod 593 away from the first rod 591 is fixedly disposed on the side wall, and an end of the first rod 591 away from the second rod 593 abuts against a side end of the bottom layer 531. When the frame 530 vibrates, the vibration of the frame 530 may be buffered by the buffer rod 590.

The side wall of the culture chamber 100 is further provided with a plurality of protruding columns 580, the protruding columns 580 are arranged on the same plane, the protruding columns 580 are arranged above the buffer rods 590, the distance from the top ends of the protruding columns 580 to the side wall of the buffer rods 590 is shorter than the distance from the top ends of the buffer rods 590 to the side wall of the buffer rods, the embryo culture and transportation device 001 further comprises a plate support 600 (refer to fig. 6), the plate support 600 can be erected on the protruding columns 580 for placing plates, and the support through holes 610 are uniformly distributed on the plate support 600. When the carrier 500 is not needed, the embryo can be cultured by placing a plate against the boss 580.

In this embodiment, the culture chamber 100 may be a square chamber or a cylindrical chamber, and the buffer rod 590 may be mounted on the side wall not only by the second rod 593, but also by the first rod 591 abutting the bottom layer 531, and the second rod 593 abutting the bottom layer 531, as long as the transport rack 500 can be placed through the culture chamber 100. Not only three buffer rods 590 but also four, five or others may be provided on each side wall of the square chamber, which may be suitably adjusted by the actual size and weight of the incubator 010.

By the embryo culture and transportation device 001 provided by the invention, temperature regulation, humidity regulation and carbon dioxide concentration regulation can be simultaneously provided in the embryo or oocyte transportation process, so that the ideal environment for embryo or oocyte survival is achieved in the culture cavity 100. Through detachable transportation frame 500 setting makes embryo culture and conveyer 001 can select installation transportation frame 500 to carry out embryo culture under the transportation state according to actual need, can provide the buffering for support body 530 through the effect of patchcord 550 and buffering pole 590 under this state, avoids embryo damage in the transportation. And when the transportation is not needed, the transportation frame 500 is taken out and directly put into a plate for static culture of embryos. The device not only can be used for embryo transportation, but also can be used for transporting active semen after the adjusting system 311 is adjusted.

Example two

The present embodiment provides an embryo culturing and transporting device 001, please refer to fig. 1 and 7, wherein the embryo culturing and transporting device 001 comprises an incubator 010, an ultrasonic generator 020, a heating element and an air passage check valve 040.

The accommodation part 300 is provided outside the bottom surface of the incubator 010, and the accommodation part 300 and the culture chamber 100 are isolated from each other by the bottom of the incubator 010. The accommodating portion 300 includes a system accommodating cavity 310 and a water tank 330, wherein an adjusting system 311 is accommodated in the system accommodating portion 300, the adjusting system 311 is electrically connected with the sensor 200, and the adjusting system 311 can receive a carbon dioxide concentration signal, a temperature signal and a humidity signal transmitted from the sensor 200, and perform analysis processing, so as to transmit corresponding adjusting signals to corresponding devices.

The water tank 330 is arranged at one side of the accommodating part 300 far away from the inductor 200, and an extending part 011 is further arranged at one side of the incubator 010 far away from the inductor 200, and the extending part 011 is hollow and is communicated with the culture cavity 100 and the water tank 330 to form an air passage 331. Through the air passage 331, water in the water tank 330 can enter the incubator 010 to adjust the humidity in the incubator 010.

In this embodiment, the heating element is a heating net 030, the heating net 030 is tiled at the bottom and the side wall of the culture cavity 100, and is covered by a metal cover to enhance the uniformity of temperature, and the current switch of the heating net 030 is controlled by the adjusting system 311 to control the temperature in the culture cavity 100.

The air passage check valve 040 is arranged on one side of the extension portion 011 away from the culture cavity 100, the outlet of the air passage check valve 040 faces the air passage 331, and the inlet of the air passage check valve 040 is connected with the premix gas device. In this embodiment, the premixed gas device is a premixed gas balloon 410, and the premixed gas balloon 410 is filled with premixed gas suitable for the carbon dioxide concentration for embryo development. The control switch of the air passage one-way valve 040 is connected with the adjusting system 311, and the air passage one-way valve 040 can be controlled to be closed or opened through the adjusting system 311 so as to control the concentration of carbon dioxide in the culture cavity 100.

Handles 050 are symmetrically arranged on the outer side of the incubator 010, and the handling of the embryo culturing and transporting device 001 is facilitated through the handles 050.

By the embryo culture and transportation device 001 provided by the invention, temperature regulation, humidity regulation and carbon dioxide concentration regulation can be simultaneously provided in the embryo or oocyte transportation process, so that the ideal environment for embryo or oocyte survival is achieved in the culture cavity 100. The device not only can be used for embryo transportation, but also can be used for transporting active semen after the adjusting system 311 is adjusted.

The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and various modifications and variations may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An embryo culturing and transporting device is characterized by comprising an incubator, an ultrasonic generator, a heating element, a gas path one-way valve and an inductor for inducing carbon dioxide concentration, temperature and humidity;

the adjusting system adjusts the humidity in the culture cavity through the ultrasonic generator;

the regulating system controls the temperature in the culture cavity through the heating element;

the ultrasonic generator is arranged in the water tank, and the heating element is arranged on one surface of the culture cavity close to the accommodating part;

the embryo culturing and transporting device further comprises a premix gas device, wherein the premix gas device comprises a premix gas storage device and a premix gas adjusting induction valve, and a gas outlet of the premix gas adjusting induction valve is connected with a gas inlet of the gas path one-way valve;

the embryo culturing and transporting device further comprises an air passage, wherein the air passage is communicated with the water tank and the side wall of the culturing cavity, and the air outlet of the air passage one-way valve is communicated with the air passage;

the regulating system controls the gas path one-way valve to be closed or opened so as to control the concentration of carbon dioxide in the culture cavity;

the embryo culturing and transporting device further comprises a transporting frame, one end, close to the opening, of the culturing cavity is provided with a slideway, the transporting frame comprises a fixing rod and a frame body used for placing a straw or a centrifugal test tube, the fixing rod is connected with the frame body through a flexible wire, and the fixing rod can be fixed in the culturing cavity through the slideway;

the culture cavity is internally provided with a buffer rod, the buffer rod comprises a first rod body and a second rod body which is sleeved in the first rod body in a sealing manner, one end of the buffer rod is fixedly connected with the inner wall of the culture cavity, and the other end of the buffer rod is abutted to the frame body.

2. The embryo culturing and transport device of claim 1, wherein the frame comprises a bottom layer and a fixed layer, the bottom layer is fixedly mounted on a surface of the fixed layer away from the fixed rod, and the bottom layer is provided with a bracket.

3. Embryo culture and transport device according to claim 2, characterized in that the fixation layer is provided with through holes cooperating with the brackets.

4. The embryo culturing and transport device of claim 1, wherein the buffer rod is provided with a plurality of posts on a side thereof adjacent to the opening, the plurality of posts being located on the same plane.

5. An embryo culturing and transporting device is characterized by comprising a handle, an incubator, an ultrasonic generator, a heating element, a gas path one-way valve and a sensor for sensing the concentration, temperature and humidity of carbon dioxide;

the culture cavity is internally provided with a buffer rod, the buffer rod comprises a first rod body and a second rod body which is hermetically sleeved in the first rod body, one end of the buffer rod is fixedly connected with the inner wall of the culture cavity, and the other end of the buffer rod is abutted to the frame body;

the handle is arranged on the outer side of the incubator.