CN116144467B - Cell culture equipment for immune cell expansion and culture method thereof - Google Patents
Cell culture equipment for immune cell expansion and culture method thereof Download PDFInfo
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- CN116144467B CN116144467B CN202310217862.9A CN202310217862A CN116144467B CN 116144467 B CN116144467 B CN 116144467B CN 202310217862 A CN202310217862 A CN 202310217862A CN 116144467 B CN116144467 B CN 116144467B
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- 238000004113 cell culture Methods 0.000 title claims abstract description 30
- 210000002865 immune cell Anatomy 0.000 title claims abstract description 30
- 230000010261 cell growth Effects 0.000 title claims abstract description 17
- 238000012136 culture method Methods 0.000 title abstract description 7
- 230000007246 mechanism Effects 0.000 claims abstract description 72
- 238000003860 storage Methods 0.000 claims abstract description 71
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 31
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 22
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 22
- 230000008569 process Effects 0.000 claims abstract description 21
- 238000005507 spraying Methods 0.000 claims abstract description 16
- 238000005192 partition Methods 0.000 claims abstract description 14
- 210000004027 cell Anatomy 0.000 claims abstract description 11
- 230000029058 respiratory gaseous exchange Effects 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 23
- 238000007789 sealing Methods 0.000 claims description 23
- 238000004140 cleaning Methods 0.000 claims description 19
- 244000005700 microbiome Species 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 8
- 230000009286 beneficial effect Effects 0.000 claims description 6
- 108091005461 Nucleic proteins Proteins 0.000 claims description 4
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 150000007523 nucleic acids Chemical class 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 230000004936 stimulating effect Effects 0.000 claims description 3
- 238000010030 laminating Methods 0.000 claims description 2
- 239000012531 culture fluid Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
Abstract
The invention relates to the technical field of cell culture equipment, in particular to cell culture equipment for immune cell expansion and a culture method thereof, which comprises a shell mechanism, a spraying mechanism, an inflation mechanism, a shaking mechanism and a wiping mechanism, wherein a second electric push rod moves rightwards to drive a culture dish to move to the right end of a partition plate, a first electric push rod drives a dropper to drop a certain amount of culture solution in a corresponding culture dish, carbon dioxide is driven to enter a gas storage tank from a first connecting tank in the rightward movement process of an air inlet pipe, after the culture solution is dropped, the second electric push rod moves leftwards to drive carbon dioxide gas in the gas storage tank to enter an immune cell culture environment through a second connecting tank, and the carbon dioxide can slowly regulate the proper environment of PH value to stimulate cell respiration so as to facilitate expansion.
Description
Technical Field
The invention relates to the technical field of cell culture equipment, in particular to cell culture equipment for immune cell expansion and a culture method thereof.
Background
Immune cells refer to cells involved in or associated with an immune response. Including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells, and the like. Immune cells can be divided into a variety of classes, and various immune cells play an important role in humans. Immune cells (immune cells) are commonly called white blood cells, and comprise congenital lymphocytes, various phagocytes and the like, lymphocytes capable of recognizing antigens and generating specific immune responses, and the like. The immune cell expansion technology is an advanced cell preparation technology in the domestic regenerative biomedical industry at present, is a tip technology of the modern service industry of the biomedical technology which is greatly supported and developed in China, and is a medical innovation mode under the high-tech support basis.
The in-process of cell culture is higher to temperature, humidity, carbon dioxide's concentration requirement, however current immune cell culture equipment is comparatively troublesome in the in-process of carrying out the cell culture in batches, inconvenient batch production is prepared, the step is more required, preparation efficiency is lower, need add the culture solution one by one in the in-process of cell culture, the in-process of adding the culture solution needs carry out manual operation on aseptic operation panel, and at the in-process of adding the culture solution leads to cell pollution easily, thereby influence cell culture quality, need mix culture solution and cell one by one after adding the culture solution, the operation is comparatively troublesome, influence the efficiency of preparation.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a cell culture device for immune cell expansion and a culture method thereof.
The technical scheme adopted for solving the technical problems is as follows: the cell culture equipment for immune cell expansion and the culture method thereof comprise a shell mechanism, a spraying mechanism, an inflation mechanism, a shaking mechanism and a wiping mechanism, wherein the spraying mechanism is arranged in the shell mechanism, one end of the spraying mechanism is provided with the inflation mechanism, one end of the inflation mechanism is provided with the shaking mechanism, and one end of the shaking mechanism is provided with the wiping mechanism.
Preferably, the shell mechanism comprises a shell, the lower end of the shell is fixedly connected with a supporting leg, the side wall of the shell is rotationally connected with a sliding door, the side wall of the interior of the shell is fixedly connected with a partition plate, and the side wall of the partition plate is provided with an opening.
Preferably, the shell mechanism further comprises a sliding groove, the sliding groove is formed in the inner side wall of the shell, the ultraviolet lamp is fixedly connected to the inner side wall of the shell, and the connecting pipe is fixedly connected to the inner side wall of the shell.
Preferably, the spraying mechanism comprises a water inlet pipe, the side wall of the water inlet pipe is fixedly connected with the shell, and one end of the water inlet pipe is fixedly connected with the liquid storage plate.
Preferably, the spraying mechanism further comprises a first electric push rod, the upper end of the liquid storage plate is fixedly connected with the first electric push rod, and the lower end of the liquid storage plate is fixedly connected with a dropper.
Preferably, the inflation mechanism comprises a second electric push rod, the side wall of the second electric push rod is fixedly connected with a first connecting rod, one end of the first connecting rod is fixedly connected with a sealing plate, one end of the sealing plate is fixedly connected with an air inlet pipe, one end of the air inlet pipe is fixedly connected with an air storage tank, the side wall of the air inlet pipe is fixedly connected with a first rubber ring, the side wall of the first rubber ring is fixedly connected with a first sliding block in a sliding manner, a first connecting groove is formed in the first sliding block, one end of the first sliding block is fixedly connected with a first spring, and one end of the first spring is fixedly connected with the air inlet pipe.
Preferably, the inflating mechanism further comprises an air outlet pipe, one end of the air storage tank is fixedly connected with the air outlet pipe, the inner side wall of the air outlet pipe is fixedly connected with a second rubber ring, the inner side wall of the second rubber ring is slidably connected with a second sliding block, a second connecting groove is formed in the second sliding block, one end of the second sliding block is fixedly connected with a second spring, one end of the second spring is fixedly connected with the inner side wall of the air outlet pipe, a template is placed at the upper end of the air storage tank, and a culture dish is placed at the upper end of the template.
Preferably, the shake mechanism includes the dead lever, dead lever one end and shell fixed connection, the first protruding piece of dead lever lateral wall fixedly connected with, first protruding piece one end laminating has the second protruding piece, second protruding piece upper end fixedly connected with fly leaf, fly leaf upper end fixedly connected with telescopic link, telescopic link upper end fixedly connected with third sliding block.
Preferably, the shaking mechanism further comprises a sleeve, the side wall of the third sliding block is in sliding connection with the sleeve, the upper end of the third sliding block is fixedly connected with a third spring, the upper end of the movable plate is fixedly connected with a second connecting rod, and the upper end of the second connecting rod is fixedly connected with a rubber block.
Preferably, the wiping mechanism comprises a third connecting rod, the third connecting rod is fixedly connected with the side wall of the air storage tank, the side wall of the third connecting rod is fixedly connected with a cleaning block, and the side wall of the cleaning block is fixedly connected with an inclined bucket.
Preferably, the method specifically comprises the following steps:
the method comprises the steps of S1, firstly placing a culture dish at the upper end of a template, arranging a plurality of groups of templates with the culture dish, enabling the templates to move rightwards through a second electric push rod, enabling the second electric push rod to move rightwards to drive a first connecting rod to move rightwards, enabling a second connecting rod to move rightwards to drive a sealing plate to move rightwards, enabling an air inlet pipe to move rightwards to drive an air storage tank to move rightwards, driving the culture dish to move to the right end of a partition plate, enabling a plurality of microorganisms to be killed through an ultraviolet lamp arranged at the upper end of the culture dish, enabling gram-negative bacteria to be most sensitive, enabling positive bacteria to be positive, enabling resistance of fungus spores to be strongest again, enabling the fungus spores to be inactivated through destroying nucleic acid, protein and the like of the microorganisms through direct effect of ultraviolet radiation, enabling the microorganisms to be killed through ozone generated through ultraviolet radiation, and enabling the culture chamber to be directly irradiated for disinfection, and being simple in use and good in effect;
s2, driving the dropper to drop to the upper end of the corresponding culture dish through the first electric push rod, introducing culture solution into the water inlet pipe, enabling the culture solution to enter the liquid storage plate in the water inlet pipe, and enabling the liquid in the liquid storage plate to drop a certain amount of culture solution in the corresponding culture dish through the dropper;
s3, in the process that the air inlet pipe moves rightwards, the first sliding block is extruded by the connecting pipe, so that the first sliding block is extruded to move leftwards in the air inlet pipe, the first sliding block moves leftwards to compress the first spring, and carbon dioxide is introduced into the connecting pipe at the moment and enters the air storage tank from the first connecting groove;
s4, after the culture solution is dripped, starting a second electric push rod to move leftwards, enabling the second electric push rod to move leftwards to drive a first connecting rod to move leftwards, enabling a second connecting rod to move leftwards to drive a sealing plate to move leftwards, enabling an air inlet pipe to move leftwards to drive an air storage tank to move leftwards, enabling the sealing plate to block an opening after the air storage tank moves leftwards, driving an air outlet pipe to move leftwards in the process of the air storage tank moving leftwards, enabling the air outlet pipe to move leftwards to drive a second sliding block to move leftwards, enabling the second sliding block to press the inner side wall of a shell to compress a second spring inwards, enabling a second connecting groove to be far away from a second rubber ring, enabling carbon dioxide gas in the air storage tank to enter an immune cell culture environment through the second connecting groove, and enabling carbon dioxide to slowly regulate proper environment for stimulating cell respiration to be beneficial to expansion;
s5, in the process of moving the air storage tank leftwards, the sleeve is driven to move leftwards, the telescopic rod is driven to move leftwards by the sleeve leftwards, the movable plate is driven to move leftwards by the telescopic rod, the second protruding block is driven to move leftwards by the movable plate, the second protruding block collides with the first protruding block, so that the movable plate is driven to shake, the movable plate shakes to drive the telescopic rod to move upwards, the telescopic rod moves upwards to drive the third sliding block to move upwards, the third sliding block moves upwards to compress the third spring, the movable plate shakes to drive the second connecting rod to shake, the second connecting rod shakes to drive the rubber block to shake, the second rubber block shakes to drive the culture dish to shake, and culture solution in the culture dish can be uniformly mixed;
s6, driving the third connecting rod to move leftwards in the process of moving the air storage tank leftwards, driving the cleaning block to move leftwards by the third connecting rod, cleaning water vapor attached to the lower end of the ultraviolet lamp by the cleaning block leftwards, and enabling the cleaned water vapor to drop at the upper end of the inclined hopper, so that the water can flow to two ends through the inclined hopper, and collecting and cleaning the water regularly.
The invention has the beneficial effects that:
(1) According to the cell culture equipment for immune cell expansion, the first electric push rod drives the dropper to drop to the upper end of the corresponding culture dish, the culture solution is introduced into the water inlet pipe, the culture solution enters the liquid storage plate inside the water inlet pipe, and a certain amount of culture solution can drop into the corresponding culture dish through the dropper.
(2) According to the cell culture equipment for immune cell expansion, carbon dioxide is driven to enter the air storage tank from the first connecting groove in the rightward movement process of the air inlet pipe, after culture solution is dripped, the second electric push rod is started to move leftwards to drive carbon dioxide gas in the air storage tank to enter an immune cell culture environment through the second connecting groove, and the carbon dioxide can slowly regulate the pH value to stimulate cell respiration in a proper environment so as to be beneficial to expansion.
(3) According to the cell culture equipment for immune cell expansion, disclosed by the invention, the culture dish is driven to shake in the leftward movement process of the air storage tank, so that culture solution in the culture dish can be uniformly mixed.
(4) According to the cell culture equipment for immune cell expansion, the third connecting rod is driven to move leftwards in the leftward movement process of the air storage tank, so that the cleaning block is driven to move leftwards to clean water vapor attached to the lower end of the ultraviolet lamp, the cleaned water vapor can drop on the upper end of the inclined hopper, and therefore water can flow to two ends through the inclined hopper to regularly collect and clean the water.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic view of the overall structure provided by the present invention;
FIG. 2 is a schematic view of the connection structure of the housing and the legs shown in FIG. 1;
FIG. 3 is a schematic view of the connection structure of the housing and the partition shown in FIG. 1;
FIG. 4 is a schematic view of a connection structure between the liquid storage plate and the dropper shown in FIG. 1;
FIG. 5 is an enlarged schematic view of portion A shown in FIG. 3;
fig. 6 is a schematic diagram illustrating a connection structure between the second electric putter and the first connecting rod shown in fig. 4;
FIG. 7 is a schematic view of a connection structure between the first rubber ring and the first slider shown in FIG. 4;
FIG. 8 is a schematic view of a connection structure between the fixing rod and the first bump block shown in FIG. 4;
fig. 9 is a schematic diagram of a connection structure between the telescopic rod and the third sliding block shown in fig. 8.
In the figure: 1. a housing mechanism; 11. a housing; 12. a support leg; 13. a sliding door; 14. a partition plate; 15. an opening; 16. a chute; 17. an ultraviolet lamp; 18. a connecting pipe; 2. a spraying mechanism; 21. a water inlet pipe; 22. a liquid storage plate; 23. a first electric push rod; 24. a dropper; 3. an inflation mechanism; 31. a second electric push rod; 32. a first connecting rod; 33. a sealing plate; 34. an air inlet pipe; 35. a gas storage tank; 36. a first rubber ring; 37. a first slider; 38. a first connection groove; 39. a first spring; 310. an air outlet pipe; 311. a second rubber ring; 312. a second slider; 313. a second connecting groove; 314. a second spring; 315. a template; 316. a culture dish; 4. a dithering mechanism; 41. a fixed rod; 42. a first bump block; 43. a second bump block; 44. a movable plate; 45. a telescopic rod; 46. a third slider; 47. a sleeve; 48. a third spring; 49. a second connecting rod; 410. a rubber block; 5. a wiping mechanism; 51. a third connecting rod; 52. cleaning the block; 53. and (5) tilting the bucket.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
As shown in fig. 1-9, the cell culture equipment for immune cell expansion and the culture method thereof according to the invention comprise a shell mechanism 1, a spraying mechanism 2, an inflation mechanism 3, a shaking mechanism 4 and a wiping mechanism 5, wherein the spraying mechanism 2 is arranged in the shell mechanism 1, one end of the spraying mechanism 2 is provided with the inflation mechanism 3, one end of the inflation mechanism 3 is provided with the shaking mechanism 4, and one end of the shaking mechanism 4 is provided with the wiping mechanism 5; the second electric push rod 31 moves rightwards to drive the culture dish 316 to move to the right end of the partition plate 14, the first electric push rod 23 drives the dropper 24 to drop a certain amount of culture solution in the corresponding culture dish 316, carbon dioxide is driven to enter the air storage tank 35 from the first connecting groove 38 in the process of moving rightwards in the air inlet pipe 34, after the culture solution is dropped, the second electric push rod 31 moves leftwards to drive carbon dioxide gas in the air storage tank 35 to enter the environment of immune cell culture through the second connecting groove 313, and the carbon dioxide can slowly regulate the proper environment of PH value to stimulate cell respiration to facilitate amplification, and drives the second rubber block 410 to shake to drive the culture dish 316 to shake in the process of moving leftwards in the air storage tank 35, so that the culture solution in the culture dish 316 can be uniformly mixed.
Preferably, the housing mechanism 1 comprises a housing 11, wherein a support leg 12 is fixedly connected to the lower end of the housing 11, a sliding door 13 is rotatably connected to the side wall of the housing 11, a partition 14 is fixedly connected to the side wall of the housing 11, an opening 15 is formed in the side wall of the partition 14, a sliding groove 16 is formed in the side wall of the housing 11, an ultraviolet lamp 17 is fixedly connected to the side wall of the housing 11, and a connecting pipe 18 is fixedly connected to the side wall of the housing 11; a variety of microorganisms can be killed by providing the ultraviolet lamp 17 at the upper end of the culture dish 316. Gram negative bacteria are most sensitive, and then positive bacteria, and then spores, are the most resistant to fungal spores. The direct action of ultraviolet rays is to inactivate nucleic acid and protein of microorganism by destroying them, the indirect action is to kill microorganism by ozone generated by ultraviolet irradiation, and the direct irradiation culture room is sterilized, the usage is simple, and the effect is good.
Preferably, the spraying mechanism 2 comprises a water inlet pipe 21, the side wall of the water inlet pipe 21 is fixedly connected with the shell 11, one end of the water inlet pipe 21 is fixedly connected with a liquid storage plate 22, the upper end of the liquid storage plate 22 is fixedly connected with a first electric push rod 23, and the lower end of the liquid storage plate 22 is fixedly connected with a dropper 24; the first electric push rod 23 drives the dropper 24 to drop to the upper end of the corresponding culture dish 316, the culture solution is introduced into the water inlet pipe 21, the culture solution enters the liquid storage plate 22 in the water inlet pipe 21, and a certain amount of culture solution can drop into the corresponding culture dish 316 through the dropper 24 by the liquid in the liquid storage plate 22.
Preferably, the inflating mechanism 3 includes a second electric push rod 31, the side wall of the second electric push rod 31 is fixedly connected with the housing 11, the side wall of the second electric push rod 31 is fixedly connected with a first connecting rod 32, one end of the first connecting rod 32 is fixedly connected with a sealing plate 33, one end of the sealing plate 33 is fixedly connected with an air inlet pipe 34, one end of the air inlet pipe 34 is fixedly connected with an air storage tank 35, the inner side wall of the air inlet pipe 34 is fixedly connected with a first rubber ring 36, the side wall of the first rubber ring 36 is fixedly connected with a first sliding block 37 in a sliding manner, a first connecting groove 38 is formed in the first sliding block 37, one end of the first sliding block 37 is fixedly connected with a first spring 39, one end of the first spring 39 is fixedly connected with the air inlet pipe 34, one end of the air storage tank 35 is fixedly connected with an air outlet pipe 310, the inner side wall of the air outlet pipe 310 is fixedly connected with a second rubber ring 311, the inner side wall of the second rubber ring 311 is fixedly connected with a second sliding block 312, a second connecting groove 313 is formed in the inner side wall of the second sliding block 312, one end of the second sliding block 312 is fixedly connected with a second spring 314, one end of the second sliding block 314 is fixedly connected with the second spring 314 and one end of the air outlet pipe 315 is fixedly connected with a template 315, and one end of the air outlet pipe 315 is fixedly connected with the template 315; placing a culture dish 316 on the upper end of a template 315, arranging a plurality of groups of templates 315 with culture dishes 316, moving rightwards through a second electric push rod 31, moving rightwards through the second electric push rod 31 to drive a first connecting rod 32 to move rightwards, moving rightwards through the second connecting rod 32 to drive a sealing plate 33 to move rightwards, moving rightwards through the sealing plate 33 to drive an air inlet pipe 34 to move rightwards, moving rightwards through the air inlet pipe 34 to drive an air storage tank 35 to move rightwards, driving the culture dish 316 to move to the right end of a partition plate 14, extruding a first sliding block 37 by a connecting pipe 18 in the rightward movement process of the air inlet pipe 34, compressing a first spring 39 by the leftward movement of the first sliding block 37, introducing carbon dioxide into the connecting pipe 18 at the moment from a first connecting groove 38 into the air storage tank 35, and after dropping culture solution, starting the second electric push rod 31 to move leftwards, the second electric push rod 31 to move leftwards drives the first connecting rod 32 to move leftwards, the second connecting rod 32 to move leftwards drives the sealing plate 33 to move leftwards, the sealing plate 33 to move leftwards drives the air inlet pipe 34 to move leftwards, the air inlet pipe 34 to move leftwards drives the air storage tank 35 to move leftwards, the air storage tank 35 can drive the sealing plate 33 to block the opening 15 after moving leftwards, the air outlet pipe 310 is driven to move leftwards in the process of moving leftwards the air storage tank 35, the air outlet pipe 310 moves leftwards to drive the second sliding block 312 to move leftwards, the second sliding block 312 is extruded with the inner side wall of the shell 11 to compress the second spring 314 inwards, at the moment, the second connecting groove 313 is far away from the second rubber ring 311, so that carbon dioxide gas in the air storage tank 35 can enter the environment of immune cell culture through the second connecting groove 313, the carbon dioxide can slowly regulate the pH value, and the proper environment for stimulating the cell respiration is beneficial to the expansion.
Preferably, the shake mechanism 4 includes a fixed rod 41, one end of the fixed rod 41 is fixedly connected with the housing 11, a first protruding block 42 is fixedly connected to a side wall of the fixed rod 41, one end of the first protruding block 42 is attached to a second protruding block 43, an upper end of the second protruding block 43 is fixedly connected with a movable plate 44, an upper end of the movable plate 44 is fixedly connected with a telescopic rod 45, an upper end of the telescopic rod 45 is fixedly connected with a third sliding block 46, a side wall of the third sliding block 46 is slidably connected with a sleeve 47, a third spring 48 is fixedly connected to an upper end of the third sliding block 46, a second connecting rod 49 is fixedly connected to an upper end of the movable plate 44, and a rubber block 410 is fixedly connected to an upper end of the second connecting rod 49; in the process of moving the air storage tank 35 leftwards, the sleeve 47 is driven to move leftwards, the sleeve 47 drives the telescopic rod 45 to move leftwards, the telescopic rod 45 drives the movable plate 44 to move leftwards, the movable plate 44 moves leftwards to drive the second protruding block 43 to move leftwards, the second protruding block 43 moves leftwards and collides with the first protruding block 42, so that the movable plate 44 can be driven to shake, the movable plate 44 shakes to drive the telescopic rod 45 to move upwards, the telescopic rod 45 moves upwards to drive the third sliding block 46 to move upwards, the third sliding block 46 moves upwards to compress the third spring 48, the movable plate 44 shakes to drive the second connecting rod 49 to shake, the second connecting rod 49 shakes to drive the rubber block 410 to shake, and the second rubber block 410 shakes to drive the culture dish 316 to shake, so that culture solution in the culture dish 316 can be evenly mixed.
Preferably, the wiping mechanism 5 includes a third connecting rod 51, the third connecting rod 51 is fixedly connected with the side wall of the air storage tank 35, the side wall of the third connecting rod 51 is fixedly connected with a cleaning block 52, and the side wall of the cleaning block 52 is fixedly connected with an inclined bucket 53; in the process of the leftward movement of the air storage tank 35, the third connecting rod 51 is driven to move leftward, the third connecting rod 51 drives the cleaning block 52 to move leftward, the cleaning block 52 moves leftward to clean water vapor attached to the lower end of the ultraviolet lamp 17, the cleaned water vapor can drop on the upper end of the inclined hopper 53, so that the water can flow to two ends through the inclined hopper 53, and water is collected and cleaned regularly.
Working principle: when the invention is used, firstly, the culture dish 316 is placed at the upper end of the template 315, a plurality of groups of templates 315 with the culture dish 316 are arranged, the second electric push rod 31 moves rightwards to drive the first connecting rod 32 to move rightwards, the second connecting rod 32 moves rightwards to drive the sealing plate 33 to move rightwards, the sealing plate 33 moves rightwards to drive the air inlet pipe 34 to move rightwards, the air inlet pipe 34 moves rightwards to drive the culture dish 316 to move to the right end of the partition plate 14, the ultraviolet lamp 17 is arranged at the upper end of the culture dish 316 to kill a plurality of microorganisms, gram-negative bacteria are most sensitive, secondly, positive bacteria are again spores, the resistance of fungus spores is strongest, the direct effect of ultraviolet rays is to kill microorganisms by destroying nucleic acid, protein and the like of the microorganisms, and the indirect effect is to kill ozone microorganisms generated by ultraviolet irradiation, and the direct irradiation of the culture chamber is used for disinfection, and the use is simple and the effect is good.
The first electric push rod 23 drives the dropper 24 to drop to the upper end of the corresponding culture dish 316, the culture solution is introduced into the water inlet pipe 21, the culture solution enters the liquid storage plate 22 in the water inlet pipe 21, and a certain amount of culture solution can drop into the corresponding culture dish 316 through the dropper 24 by the liquid in the liquid storage plate 22.
During the rightward movement of the air inlet pipe 34, the first sliding block 37 is pressed by the connecting pipe 18, so that the first sliding block 37 is pressed to move leftward in the air inlet pipe 34, the first sliding block 37 moves leftward to compress the first spring 39, and carbon dioxide is introduced into the connecting pipe 18 and enters the air storage tank 35 from the first connecting groove 38.
After the culture solution is dripped, the second electric push rod 31 is started to move leftwards, the second electric push rod 31 moves leftwards to drive the first connecting rod 32 to move leftwards, the second connecting rod 32 moves leftwards to drive the sealing plate 33 to move leftwards, the sealing plate 33 moves leftwards to drive the air inlet pipe 34 to move leftwards, the air inlet pipe 34 moves leftwards to drive the air storage tank 35 to block the opening 15 after the air storage tank 35 moves leftwards, the air outlet pipe 310 is driven to move leftwards in the process of the air storage tank 35 to move leftwards, the air outlet pipe 310 moves leftwards to drive the second sliding block 312 to move leftwards, the second sliding block 312 presses the second spring 314 inwards by pressing with the inner side wall of the shell 11, at the moment, the second connecting groove 313 is far away from the second rubber ring 311, and thus carbon dioxide gas inside the air storage tank 35 can enter the environment for immune cell culture through the second connecting groove 313, and the appropriate environment for adjusting the PH value of carbon dioxide can stimulate cell respiration more slowly to be beneficial to expansion.
In the process of moving the air storage tank 35 leftwards, the sleeve 47 is driven to move leftwards, the sleeve 47 moves leftwards to drive the telescopic rod 45 to move leftwards, the telescopic rod 45 moves leftwards to drive the movable plate 44 to move leftwards, the movable plate 44 moves leftwards to drive the second convex block 43 to move leftwards, the second convex block 43 collides with the first convex block 42, so that the movable plate 44 can be driven to shake, the movable plate 44 shakes to drive the telescopic rod 45 to move upwards, the telescopic rod 45 moves upwards to drive the third sliding block 46 to move upwards, the third sliding block 46 moves upwards to compress the third spring 48, the movable plate 44 shakes to drive the second connecting rod 49 to shake, the second connecting rod 49 shakes to drive the rubber block 410 to shake, the second rubber block 410 shakes to drive the culture dish 316 to shake, and culture solution in the culture dish 316 can be evenly mixed.
In the process of the leftward movement of the air storage tank 35, the third connecting rod 51 is driven to move leftward, the third connecting rod 51 drives the cleaning block 52 to move leftward, the cleaning block 52 moves leftward to clean water vapor attached to the lower end of the ultraviolet lamp 17, the cleaned water vapor can drop on the upper end of the inclined hopper 53, so that the water can flow to two ends through the inclined hopper 53, and water is collected and cleaned regularly.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the foregoing examples, and that the foregoing description and description are merely illustrative of the principles of this invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A cell culture apparatus for immune cell expansion, characterized by: the novel cleaning device comprises a shell mechanism (1), a spraying mechanism (2), an inflation mechanism (3), a shaking mechanism (4) and a wiping mechanism (5), wherein the spraying mechanism (2) is arranged in the shell mechanism (1), the inflation mechanism (3) is arranged at one end of the spraying mechanism (2), the shaking mechanism (4) is arranged at one end of the inflation mechanism (3), and the wiping mechanism (5) is arranged at one end of the shaking mechanism (4);
the shell mechanism (1) comprises a shell (11), the lower end of the shell (11) is fixedly connected with a supporting leg (12), the side wall of the shell (11) is rotationally connected with a sliding door (13), the side wall of the interior of the shell (11) is fixedly connected with a partition plate (14), and an opening (15) is formed in the side wall of the partition plate (14);
the shell mechanism (1) further comprises a sliding groove (16), the sliding groove (16) is formed in the inner side wall of the shell (11), the ultraviolet lamp (17) is fixedly connected to the inner side wall of the shell (11), and the connecting pipe (18) is fixedly connected to the inner side wall of the shell (11);
the spraying mechanism (2) comprises a water inlet pipe (21), the side wall of the water inlet pipe (21) is fixedly connected with the shell (11), and one end of the water inlet pipe (21) is fixedly connected with a liquid storage plate (22);
the spraying mechanism (2) further comprises a first electric push rod (23), the upper end of the liquid storage plate (22) is fixedly connected with the first electric push rod (23), and the lower end of the liquid storage plate (22) is fixedly connected with a dropper (24);
the inflation mechanism (3) comprises a second electric push rod (31), the side wall of the second electric push rod (31) is fixedly connected with the shell (11), the side wall of the second electric push rod (31) is fixedly connected with a first connecting rod (32), one end of the first connecting rod (32) is fixedly connected with a sealing plate (33), one end of the sealing plate (33) is fixedly connected with an air inlet pipe (34), one end of the air inlet pipe (34) is fixedly connected with an air storage tank (35), the side wall of the air inlet pipe (34) is fixedly connected with a first rubber ring (36), the side wall of the first rubber ring (36) is fixedly connected with a first sliding block (37) in a sliding manner, a first connecting groove (38) is formed in the first sliding block (37), one end of the first sliding block (37) is fixedly connected with a first spring (39), and one end of the first spring (39) is fixedly connected with the air inlet pipe (34);
the utility model discloses a gas holder (35) of air inflation mechanism, including gas holder (35) including gas inlet pipe, gas holder (35) one end fixedly connected with outlet pipe (310), inside lateral wall fixedly connected with second rubber circle (311) of outlet pipe (310), inside lateral wall sliding connection of second rubber circle (311) has second sliding block (312), second spread groove (313) have been seted up to second sliding block (312) inside, second sliding block (312) one end fixedly connected with second spring (314), second spring (314) one end and the inside lateral wall fixed connection of outlet pipe (310), template (315) have been placed to gas holder (35) upper end, culture dish (316) have been placed to template (315) upper end.
2. A cell culture apparatus for immune cell expansion according to claim 1, wherein: the shake mechanism (4) comprises a fixed rod (41), fixed rod (41) one end and shell (11) fixed connection, first protruding piece (42) of fixed rod (41) lateral wall fixedly connected with, first protruding piece (42) one end laminating has second protruding piece (43), second protruding piece (43) upper end fixedly connected with fly leaf (44), fly leaf (44) upper end fixedly connected with telescopic link (45), telescopic link (45) upper end fixedly connected with third sliding block (46).
3. A cell culture apparatus for immune cell expansion according to claim 2, wherein: the shaking mechanism (4) further comprises a sleeve (47), the side wall of the third sliding block (46) is in sliding connection with the sleeve (47), the upper end of the third sliding block (46) is fixedly connected with a third spring (48), the upper end of the movable plate (44) is fixedly connected with a second connecting rod (49), and the upper end of the second connecting rod (49) is fixedly connected with a rubber block (410).
4. A cell culture apparatus for immune cell expansion according to claim 3, wherein: the wiping mechanism (5) comprises a third connecting rod (51), the third connecting rod (51) is fixedly connected with the side wall of the air storage tank (35), the side wall of the third connecting rod (51) is fixedly connected with a cleaning block (52), and the side wall of the cleaning block (52) is fixedly connected with an inclined hopper (53).
5. A method of using a cell culture apparatus for immune cell expansion according to claim 4, wherein: the method specifically comprises the following steps:
the method comprises the steps of S1, firstly, placing a culture dish (316) at the upper end of a template (315), arranging a plurality of groups of templates (315) with the culture dish (316), moving rightwards through a second electric push rod (31), driving a first connecting rod (32) to move rightwards through the rightwards movement of the second electric push rod (31), driving a sealing plate (33) to move rightwards through the rightwards movement of the second connecting rod (32), driving an air inlet pipe (34) to move rightwards through the rightwards movement of the sealing plate (33), driving an air storage tank (35) to move rightwards through the air inlet pipe (34), driving the culture dish (316) to move to the right end of a partition plate (14), killing various microorganisms through an ultraviolet lamp (17) arranged at the upper end of the culture dish (316), wherein the gram-negative bacteria are the most sensitive, the gram-negative bacteria are the positive bacteria, the spore is the strongest again, the direct action of ultraviolet rays is to kill microorganisms through destroying nucleic acid and protein of the microorganisms, and the indirect action is to kill ozone microorganisms generated through ultraviolet irradiation, and the direct irradiation of the microorganisms, so that the effect is simple;
s2, driving a dropper (24) to drop to the upper end of a corresponding culture dish (316) through a first electric push rod (23), introducing culture solution into the water inlet pipe (21), enabling the culture solution to enter the liquid storage plate (22) in the water inlet pipe (21), and enabling a certain amount of culture solution in the liquid storage plate (22) to drop into the corresponding culture dish (316) through the dropper (24);
s3, in the process of rightward movement of the air inlet pipe (34), the first sliding block (37) is extruded by the connecting pipe (18), so that the first sliding block (37) is extruded to move leftwards in the air inlet pipe (34), the first sliding block (37) moves leftwards to compress the first spring (39), and at the moment, carbon dioxide is introduced into the connecting pipe (18) and enters the air storage tank (35) from the first connecting groove (38);
s4, after the culture solution is dripped, starting a second electric push rod (31) to move leftwards, enabling the second electric push rod (31) to move leftwards to drive a first connecting rod (32) to move leftwards, enabling a second connecting rod (32) to move leftwards to drive a sealing plate (33) to move leftwards, enabling an air inlet pipe (34) to move leftwards to drive an air storage tank (35) to move leftwards, enabling the air storage tank (35) to drive the sealing plate (33) to block an opening (15) after the air storage tank (35) moves leftwards, driving an air outlet pipe (310) to move leftwards in the process of moving the air storage tank (35), enabling the air outlet pipe (310) to move leftwards to drive a second sliding block (312) to move leftwards, enabling the second sliding block (312) to press an inner side wall of a shell (11) to inwards compress a second spring (314), enabling a second connecting groove (313) to be far away from a second rubber ring (311), enabling carbon dioxide gas inside the air storage tank (35) to enter an environment for immune cell culture through the second connecting groove (313), and enabling carbon dioxide to slowly regulate a proper environment for stimulating cell respiration to be beneficial to expand PH value;
s5, in the process of leftward movement of the air storage tank (35), the sleeve (47) is driven to leftward movement, the telescopic rod (45) is driven to leftward movement, the movable plate (44) is driven to leftward movement, the second protruding block (43) is driven to leftward movement to collide with the first protruding block (42), so that the movable plate (44) can be driven to shake, the movable plate (44) shakes to drive the telescopic rod (45) to upward movement, the telescopic rod (45) moves upward to drive the third sliding block (46) to upward movement, the third sliding block (46) moves upward to compress the third spring (48), the movable plate (44) shakes to drive the second connecting rod (49) to shake, the second connecting rod (49) shakes to drive the rubber block (410) to shake, and the second rubber block (410) shakes to drive the culture dish (316) to shake, so that culture fluid in the culture dish (316) can be uniformly mixed;
s6, in the process of leftward movement of the air storage tank (35), the third connecting rod (51) is driven to leftward movement of the cleaning block (52), the cleaning block (52) moves leftward to clean water vapor attached to the lower end of the ultraviolet lamp (17), the cleaned water vapor can drop on the upper end of the inclined hopper (53), and accordingly water can flow to two ends through the inclined hopper (53) to collect and clean water periodically.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN213357608U (en) * | 2020-08-05 | 2021-06-04 | 天津瀛诺科技发展有限公司 | Incubator for cell culture |
| WO2021114232A1 (en) * | 2019-12-12 | 2021-06-17 | 生合生物科技（扬州）有限公司 | Automatically controlled strain culture dish |
| CN114350516A (en) * | 2022-01-13 | 2022-04-15 | 河北因赛乐生物科技有限公司 | Culture apparatus for immune cell culture |
| CN115232746A (en) * | 2022-08-25 | 2022-10-25 | 济宁市技师学院 | Modularized bioreactor suitable for culturing multiple cell types |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP6405690B2 (en) * | 2014-05-09 | 2018-10-17 | 東洋製罐グループホールディングス株式会社 | Multi-chamber culture container and cell culture method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021114232A1 (en) * | 2019-12-12 | 2021-06-17 | 生合生物科技（扬州）有限公司 | Automatically controlled strain culture dish |
| CN213357608U (en) * | 2020-08-05 | 2021-06-04 | 天津瀛诺科技发展有限公司 | Incubator for cell culture |
| CN114350516A (en) * | 2022-01-13 | 2022-04-15 | 河北因赛乐生物科技有限公司 | Culture apparatus for immune cell culture |
| CN115232746A (en) * | 2022-08-25 | 2022-10-25 | 济宁市技师学院 | Modularized bioreactor suitable for culturing multiple cell types |
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