CN115232746A

CN115232746A - Modularized bioreactor suitable for culturing multiple cell types

Info

Publication number: CN115232746A
Application number: CN202211022484.0A
Authority: CN
Inventors: 李春
Original assignee: Jining Technician College
Current assignee: Jining Technician College
Priority date: 2022-08-25
Filing date: 2022-08-25
Publication date: 2022-10-25

Abstract

The invention discloses a modularized bioreactor suitable for culturing various cell types, which comprises a reactor shell, wherein the lower end of the reactor shell is symmetrically provided with a supporting base, and the upper end of the reactor shell is attached with a sealing cover plate; further comprising: and the second sealing plate is arranged on the inner surface of the sealing cover plate, is tightly attached to a groove formed in the bearing plate, and is arranged on the inner surface of the reactor shell. This modularization bioreactor suitable for multiple cell type is cultivateed, can realize adding culture solution and required gas to reactor shell inside through intake pipe and passage, rotation through rotating the screw rod, to the position change of accepting slider and drive board, the realization is to steady putting into of culture dish and take out, work through electric telescopic handle, make the rotor plate rotate, thereby the realization is to the change of position of PH value detection device, be convenient for measure the inside PH value of culture dish, no longer need the manual work to place the measurement.

Description

Modularized bioreactor suitable for culturing multiple cell types

Technical Field

The invention relates to the technical field of bioreactors, in particular to a modular bioreactor suitable for culturing various cell types.

Background

The bioreactor is a device capable of simulating the cell growth environment, and can ensure the cell culture through the bioreactor, thereby realizing the observation of the growth condition of the cell, generally needing to carry out modular control on the bioreactor for reducing the experimental variable, realizing the culture of the cells of different types, and creating the environment suitable for the life of the cells of different types.

However, there are some problems in the process of using the bioreactor, for example, when the common bioreactor is added with culture solution and required gas, a cover plate at the upper end of the bioreactor needs to be opened, in the process, the sterility inside the bioreactor is difficult to ensure, thereby causing certain influence on the culture of cells, and the culture dish inside the common bioreactor adopts a direct placement mode, thereby in the process of moving the bioreactor, the culture dish is very easy to move, and when the position of the culture dish moves, in the process of adding the culture solution, it is difficult to ensure that all the culture dish is added.

In order to solve the problems, innovative design based on the original bioreactor is urgently needed.

Disclosure of Invention

The invention aims to provide a modularized bioreactor suitable for culturing various cell types, and aims to solve the problems that a cover plate at the upper end of the bioreactor needs to be opened when culture solution and required gas are added, the sterility of the interior of the bioreactor is difficult to ensure, and a culture dish is easy to move in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a modularized bioreactor suitable for culturing various cell types comprises a reactor shell, wherein the lower end of the reactor shell is symmetrically provided with a supporting base, the upper end of the reactor shell is attached with a sealing cover plate, the lower end of the sealing cover plate is provided with a first sealing plate, the first sealing plate is tightly attached to the reactor shell through a groove formed in the reactor shell, and the outer surface of the reactor shell is provided with a display device and a control button;

further comprising:

the second sealing plate is arranged on the inner surface of the sealing cover plate, is tightly attached to a groove formed in the bearing plate, and is arranged on the inner surface of the reactor shell;

the rotary cover plate is in threaded connection with a protruding plate arranged on the sealing cover plate through a threaded structure arranged inside the rotary cover plate;

the limiting plate is arranged at the lower end of the sealing cover plate;

the fixed plate is arranged on the outer surface of the reactor shell, and the limiting plate penetrates through the fixed plate;

the positioning rod penetrates through the fixing plate and is in clamping connection with the limiting plate through a groove formed in the limiting plate;

the positioning spring is arranged on the outer surface of the fixing plate and is mutually connected with the positioning rod;

the temperature detection device is arranged at the left lower end of the sealing cover plate, and the humidity detection device is arranged at the right lower end of the sealing cover plate;

the air inlet pipe penetrates through the sealing cover plate, is connected with the connecting plate, and is provided with an air supply plate and an additional installation plate;

the bottom end plate is arranged at the lower end of the connecting plate, is mutually connected with the driving plate through a mounting screw and is provided with the temperature detection device and the humidity detection device at the lower end;

a motor disposed on a plate-like structure external to the reactor housing;

the electric telescopic rod is arranged at the lower end of the installation plate;

the feeding pipe is installed on the connecting plate, connected with the feeding pipe, and penetrates through the sealing cover plate.

Preferably, the connecting plate with install board interconnect additional, just install the board upper end additional and install the block board, and the symmetry is provided with the fixed reed on the block board, through above-mentioned structure, be convenient for carry out the block through the fixed reed to placing the board and fix.

Preferably, the fixed reed is installed in a plate-shaped structure arranged at the lower end of the placing plate, the culture dish is installed at the upper end of the placing plate, and the culture dish is convenient to install and detach through the structure.

Preferably, the carrying sliding blocks are symmetrically arranged on the driving plate and are arranged in a T shape, the carrying sliding blocks are in sliding connection with the reactor shell through grooves formed in the reactor shell, and the moving range of the driving plate is limited conveniently through the carrying sliding blocks through the structure.

Preferably, the receiving slide block is connected with the rotating screw rod through a threaded hole formed in the upper end of the receiving slide block, the rotating screw rod and the reactor shell form a rotating mechanism through a bearing installed on the reactor shell, and the rotating screw rod is supported without being influenced by the rotation of the rotating screw rod through the structure.

Preferably, the lower end of the rotating screw is provided with a first bevel gear, the first bevel gear is meshed with a second bevel gear, the diameters of the first bevel gear and the second bevel gear are equal to the number of teeth of the first bevel gear and the second bevel gear, and the consistency of movement is convenient to guarantee through the structure.

Preferably, the second bevel gear is mounted on a rotating shaft, the rotating shaft and the reactor shell form a rotating mechanism through a bearing mounted on the reactor shell, the rotating shaft and the output end of the motor are connected with each other, and the rotating shaft is convenient to drive the second bevel gear to rotate.

Preferably, electric telescopic handle and removal slider interconnect, just remove the slider and constitute sliding connection with the platelike structure that installs the board lower extreme additional and set up, and the platelike structure that removes the slider lower extreme and set up installs fixed rotating shaft, through above-mentioned structure, be convenient for drive through electric telescopic handle's work and remove the slider.

Preferably, the fixed rotating shaft is rotatably connected with the rotating plate through a groove formed in the rotating plate, the plate-shaped structures connected with the rotating plate are slidably connected with the PH value detection device through a sliding block, and the PH value detection device is driven to move up and down through the rotation of the rotating plate.

Compared with the prior art, the invention has the beneficial effects that:

(1) The modularized bioreactor suitable for culturing various cell types is provided with a first sealing plate, a limiting plate, an air inlet pipe and a material guide pipe, the sealing property between a reactor shell and a sealing cover plate can be increased through the first sealing plate and the limiting plate, the sealing cover plate is clamped and fixed through a positioning rod, the situation that the sealing cover plate is not in thorough contact with the reactor shell is avoided in the using process, culture solution and required gas can be added into the reactor shell through the air inlet pipe and the material guide pipe, the sealing cover plate does not need to be opened any more, the sealing cover plate is less in contact with the outside, and the sterility of the interior of the reactor shell can be effectively guaranteed;

(2) The modularized bioreactor suitable for culturing various cell types is provided with a rotating screw rod, a receiving sliding block and a driving plate, a bevel gear is driven to rotate through a motor, so that the rotating screw rod starts to rotate, the positions of the receiving sliding block and the driving plate can be changed up and down along with the rotation of the rotating screw rod, namely the position of a culture dish is changed, and the culture dish can be stably placed into the bioreactor and taken out;

(3) This modularization bioreactor suitable for multiple cell type is cultivateed is provided with electric telescopic handle, rotor plate and PH value detection device, and through electric telescopic handle's work this moment, it removes to drive the removal slider to drive the rotor plate and begin to rotate around fixed rotating shaft, thereby change about the realization is carried out PH value detection device's position, be convenient for measure the inside PH value of culture dish, no longer need the manual work to place the measurement, convenient and fast more.

Drawings

FIG. 1 is a schematic sectional view of the reactor shell in a front view;

FIG. 2 is a schematic front view of a display device according to the present invention;

FIG. 3 is a schematic bottom sectional view of the first seal plate of the present invention;

FIG. 4 is a schematic top sectional view of the culture dish of the present invention;

FIG. 5 is an enlarged view of the structure at A in FIG. 1 according to the present invention;

FIG. 6 is an enlarged view of the structure at B in FIG. 1 according to the present invention;

FIG. 7 is a schematic view of a sectional structure of a receiving slider according to the present invention;

fig. 8 is a perspective view of the receiving plate of the present invention.

In the figure: 1. a reactor housing; 2. a support base; 3. sealing the cover plate; 4. a first sealing plate; 5. a second sealing plate; 6. a bearing plate; 7. a display device; 8. a control button; 9. rotating the cover plate; 10. a limiting plate; 11. a fixing plate; 12. positioning a rod; 13. a positioning spring; 14. a temperature detection device; 15. a humidity detection device; 16. an air inlet pipe; 17. a connecting plate; 18. a gas supply plate; 19. a bottom end plate; 20. mounting screws; 21. driving the plate; 22. carrying the sliding block; 23. rotating the screw; 24. a first bevel gear; 25. a second bevel gear; 26. a rotating shaft; 27. an electric motor; 28. adding a plate; 29. a clamping plate; 30. fixing the reed; 31. placing the plate; 32. a culture dish; 33. an electric telescopic rod; 34. moving the slide block; 35. fixing the rotating shaft; 36. a rotating plate; 37. a pH value detection device; 38. a feed tube; 39. a material guide pipe.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-8, the present invention provides a technical solution: a modularized bioreactor suitable for culturing various cell types comprises a reactor shell 1, wherein the lower end of the reactor shell is symmetrically provided with supporting bases 2, the upper end of the reactor shell 1 is attached with a sealing cover plate 3, the lower end of the sealing cover plate 3 is provided with a first sealing plate 4, the first sealing plate 4 is tightly attached to the reactor shell 1 through a groove formed in the reactor shell 1, and the outer surface of the reactor shell 1 is provided with a display device 7 and a control button 8;

further comprising:

the second sealing plate 5 is arranged on the inner surface of the sealing cover plate 3, the second sealing plate 5 is tightly attached to a groove formed in the bearing plate 6, and the bearing plate 6 is arranged on the inner surface of the reactor shell 1;

the rotary cover plate 9 is in threaded connection with a convex plate arranged on the sealing cover plate 3 through a thread structure arranged inside;

the limiting plate 10 is arranged at the lower end of the sealing cover plate 3;

the fixed plate 11 is arranged on the outer surface of the reactor shell 1, and a limiting plate 10 penetrates through the fixed plate 11;

the positioning rod 12 penetrates through the fixing plate 11, and the positioning rod 12 is in clamping connection with the limiting plate 10 through a groove formed in the limiting plate 10;

a positioning spring 13 mounted on the outer surface of the fixing plate 11, the positioning spring 13 and the positioning rod 12 being connected to each other;

the temperature detection device 14 is arranged at the left lower end of the sealing cover plate 3, and the humidity detection device 15 is arranged at the right lower end of the sealing cover plate 3;

the air inlet pipe 16 penetrates through the sealing cover plate 3, the air inlet pipe 16 is connected with the connecting plate 17, and an air supply plate 18 and an additional installation plate 28 are installed on the connecting plate 17;

a bottom end plate 19 installed at the lower end of the connection plate 17, the bottom end plate 19 being interconnected with a driving plate 21 by means of mounting screws 20, and the temperature sensing device 14 and the humidity sensing device 15 being installed at the lower end of the driving plate 21;

a motor 27 arranged on the plate-like structure outside the reactor housing 1;

an electric telescopic rod 33 arranged at the lower end of the mounting plate 28;

the feeding pipe 38 is arranged on the connecting plate 17, the feeding pipe 38 and the material guiding pipe 39 are connected with each other, and the material guiding pipe 39 penetrates through the sealing cover plate 3;

in this example, the connecting plate 17 is connected with the additional plate 28, the upper end of the additional plate 28 is provided with a clamping plate 29, and the clamping plate 29 is symmetrically provided with fixed reeds 30; the fixed reed 30 is arranged in a plate-shaped structure arranged at the lower end of the placing plate 31, and the culture dish 32 is arranged at the upper end of the placing plate 31;

when culture solution needs to be added into the culture dish 32, the rotary cover plate 9 is rotated, the rotary cover plate 9 starts to move in the direction away from the sealing cover plate 3 until the rotary cover plate 9 is no longer connected with the convex plate arranged on the sealing cover plate 3, at the moment, the culture solution can be added into the guide pipe 39, then the culture solution can enter the culture dish 32 through the feed pipe 38, so that the culture solution can be added into the culture dish 32, and the required gas is filled into the gas inlet pipe 16, at the moment, the gas in the gas inlet pipe 16 supplies gas to the culture dish 32 through the gas supply plate 18, so that the normal growth of cells is ensured, because the temperature detection device 14 and the humidity detection device 15 are arranged at the lower end of the sealing cover plate 3 and the lower end of the driving plate 21, the temperature and the humidity inside the reactor shell 1 can be detected through the temperature detection device 14 and the humidity detection device 15, so that the temperature and the humidity inside the reactor shell 1 can be the temperature and the humidity required for the growth of the cells can be ensured, and the temperature and the humidity inside the reactor shell 1 can be adjusted through the display device 7 and the control button 8;

the driving plate 21 is symmetrically provided with receiving sliding blocks 22, the receiving sliding blocks 22 are arranged in a T shape, and the receiving sliding blocks 22 are in sliding connection with the reactor shell 1 through grooves formed in the reactor shell 1; the bearing slide block 22 is connected with the rotating screw 23 through a threaded hole formed in the upper end of the bearing slide block, and the rotating screw 23 and the reactor shell 1 form a rotating mechanism through a bearing arranged on the reactor shell 1; the lower end of the rotating screw 23 is provided with a first bevel gear 24, the first bevel gear 24 is meshed with a second bevel gear 25, and the diameters and the gear teeth numbers of the first bevel gear 24 and the second bevel gear 25 are equal; the second bevel gear 25 is mounted on a rotating shaft 26, and the rotating shaft 26 constitutes a rotating mechanism with the reactor housing 1 through a bearing mounted on the reactor housing 1, and the rotating shaft 26 and an output end of the motor 27 are connected to each other;

when the culture dish 32 needs to be taken out, the positioning rod 12 is pulled to make the positioning rod 12 start to move towards the direction away from the fixing plate 11, at this time, the positioning spring 13 mounted on the positioning rod 12 starts to be stretched and deformed until the positioning rod 12 is no longer attached to the groove formed on the limiting plate 10, at this time, the sealing cover plate 3 is no longer fastened and fixed, then the sealing cover plate 3 can be pulled upwards, then the joint at the upper end of the air inlet pipe 16 is rotated, so that the air inlet pipe 16 is detached from the tubular structure arranged at the upper end of the sealing cover plate 3, at this time, the sealing cover plate 3 can be detached, then the motor 27 starts to operate, because the output end of the motor 27 is connected with the rotating shaft 26, and the rotating shaft 26 forms a rotating mechanism with the reactor shell 1 through a sealing bearing, at this time, the rotating shaft 26 starts to rotate, as the rotating gear 25 mounted on the rotating shaft 26 also starts to rotate, because the rotating gear 25 is meshed with the first bevel gear 24, at this time, the first bevel gear 24 also starts to rotate, because the first bevel gear 24 is mounted at the lower end of the rotating screw 23, as the rotating screw 23 rotates, the rotating shaft 23 is connected with the screw 23, the screw bearing and the second bevel gear 23, the sliding block 22 is mounted on the fixed, so as the culture dish 23, the culture plate 22 starts to move, the culture dish 32 starts to move, and the culture plate 31, so as the culture plate 22 starts to move, the culture dish 32, the plate 22 starts to be placed on the plate, and the plate 22, and the plate 31, the plate 22 starts to move, and the plate 22 is placed, and the plate 22 is placed, at this time, thereby realizing the disassembly of the culture dish 32;

the electric telescopic rod 33 is connected with the movable sliding block 34, the movable sliding block 34 is in sliding connection with a plate-shaped structure arranged at the lower end of the installing plate 28, and a fixed rotating shaft 35 is arranged on the plate-shaped structure arranged at the lower end of the movable sliding block 34; the fixed rotating shaft 35 is in rotating connection with the rotating plate 36 through a groove formed in the rotating plate 36, and a plate-shaped structure formed by mutually connecting the rotating plates 36 is in sliding connection with the PH value detection device 37 through a sliding block;

when the PH value of needs in to culture dish 32 detects, make electric telescopic handle 33 begin work, electric telescopic handle 33 just drives its interconnect's platelike structure and begins to slide under the effect of removal slider 34 this moment, rotating plate 36 just begins to rotate around fixed rotating shaft 35 this moment, along with the rotation of rotating plate 36 this moment, alright realize changing about the position of PH value detection device 37, until PH value detection device 37 and the inside culture solution of culture dish 32 contact each other, alright detect the PH value of the inside culture solution of culture dish 32 this moment.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A modularized bioreactor suitable for culturing various cell types comprises a reactor shell (1), wherein the lower end of the reactor shell is symmetrically provided with supporting bases (2), the upper end of the reactor shell (1) is attached with a sealing cover plate (3), the lower end of the sealing cover plate (3) is provided with a first sealing plate (4), the first sealing plate (4) is tightly attached to the reactor shell (1) through a groove formed in the reactor shell (1), and the outer surface of the reactor shell (1) is provided with a display device (7) and a control button (8);

it is characterized by also comprising:

the second sealing plate (5) is arranged on the inner surface of the sealing cover plate (3), the second sealing plate (5) is tightly attached to a groove formed in the bearing plate (6), and the bearing plate (6) is arranged on the inner surface of the reactor shell (1);

the rotary cover plate (9) is in threaded connection with a protruding plate arranged on the sealing cover plate (3) through a thread structure arranged inside;

the limiting plate (10) is arranged at the lower end of the sealing cover plate (3);

the fixing plate (11) is arranged on the outer surface of the reactor shell (1), and the limiting plate (10) penetrates through the fixing plate (11);

the positioning rod (12) penetrates through the fixing plate (11), and the positioning rod (12) is in clamping connection with the limiting plate (10) through a groove formed in the limiting plate (10);

the positioning spring (13) is arranged on the outer surface of the fixing plate (11), and the positioning spring (13) is connected with the positioning rod (12);

the temperature detection device (14) is arranged at the left lower end of the sealing cover plate (3), and the humidity detection device (15) is arranged at the right lower end of the sealing cover plate (3);

the air inlet pipe (16) penetrates through the sealing cover plate (3), the air inlet pipe (16) is connected with the connecting plate (17), and an air supply plate (18) and an additional installation plate (28) are installed on the connecting plate (17);

the bottom end plate (19) is arranged at the lower end of the connecting plate (17), the bottom end plate (19) is connected with the driving plate (21) through a mounting screw (20), and the temperature detection device (14) and the humidity detection device (15) are arranged at the lower end of the driving plate (21);

-an electric motor (27) arranged on a plate-like structure outside the reactor housing (1);

an electric telescopic rod (33) arranged at the lower end of the additional plate (28);

and the feeding pipe (38) is installed on the connecting plate (17), the feeding pipe (38) and the material guiding pipe (39) are connected with each other, and the material guiding pipe (39) penetrates through the sealing cover plate (3).

2. The modular bioreactor of claim 1, wherein the bioreactor comprises: the connecting plate (17) and the installing plate (28) are connected with each other, the upper end of the installing plate (28) is provided with a clamping plate (29), and the clamping plate (29) is symmetrically provided with fixing reeds (30).

3. A modular bioreactor adapted for the cultivation of multiple cell types, according to claim 2, wherein: the fixing reed (30) is arranged in a plate-shaped structure arranged at the lower end of the placing plate (31), and a culture dish (32) is arranged at the upper end of the placing plate (31).

4. The modular bioreactor of claim 1, wherein the bioreactor comprises: the driving plate (21) is symmetrically provided with bearing sliding blocks (22), the bearing sliding blocks (22) are arranged in a T shape, and the bearing sliding blocks (22) are in sliding connection with the reactor shell (1) through grooves formed in the reactor shell (1).

5. A modular bioreactor adapted for the cultivation of multiple cell types, according to claim 4, wherein: the bearing sliding block (22) is connected with the rotating screw rod (23) through a threaded hole formed in the upper end of the bearing sliding block, and the rotating screw rod (23) and the reactor shell (1) form a rotating mechanism through a bearing arranged on the reactor shell (1).

6. A modular bioreactor adapted for the cultivation of multiple cell types, according to claim 5, wherein: the lower end of the rotating screw rod (23) is provided with a first bevel gear (24), the first bevel gear (24) and a second bevel gear (25) are meshed with each other, and the diameters and the number of teeth of the first bevel gear (24) and the second bevel gear (25) are equal.

7. A modular bioreactor adapted for the cultivation of multiple cell types, according to claim 6, wherein: the second bevel gear (25) is mounted on a rotating shaft (26), the rotating shaft (26) and the reactor housing (1) form a rotating mechanism through a bearing mounted on the reactor housing (1), and the rotating shaft (26) and an output end of the motor (27) are connected with each other.

8. The modular bioreactor of claim 1, wherein the bioreactor comprises: the electric telescopic rod (33) is connected with the movable sliding block (34) mutually, the movable sliding block (34) is in sliding connection with a plate-shaped structure arranged at the lower end of the installing plate (28), and a fixed rotating shaft (35) is arranged on the plate-shaped structure arranged at the lower end of the movable sliding block (34).

9. The modular bioreactor of claim 8, wherein the bioreactor comprises: the fixed rotating shaft (35) is in rotating connection with the rotating plate (36) through a groove formed in the rotating plate (36), and the plate-shaped structures connected with the rotating plate (36) are in sliding connection with the PH value detection device (37) through a sliding block.