CN114292750A - Constant-temperature culture device capable of being observed in real time - Google Patents
Constant-temperature culture device capable of being observed in real time Download PDFInfo
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- CN114292750A CN114292750A CN202210093226.5A CN202210093226A CN114292750A CN 114292750 A CN114292750 A CN 114292750A CN 202210093226 A CN202210093226 A CN 202210093226A CN 114292750 A CN114292750 A CN 114292750A
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- 230000003287 optical effect Effects 0.000 claims abstract description 61
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims abstract description 17
- 238000010438 heat treatment Methods 0.000 claims description 22
- 238000004321 preservation Methods 0.000 claims description 15
- 238000003384 imaging method Methods 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 4
- 239000003381 stabilizer Substances 0.000 claims 1
- 238000004113 cell culture Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 210000002257 embryonic structure Anatomy 0.000 description 6
- 238000009413 insulation Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
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Abstract
The invention belongs to the technical field of cell culture devices, and particularly relates to a constant-temperature culture device capable of realizing real-time observation, which comprises a three-way adjusting observation platform, a three-way adjusting mechanism and an optical assembly, wherein the three-way adjusting mechanism and the optical assembly are arranged inside the three-way adjusting observation platform; compared with the prior art, the constant-temperature culture device capable of being observed in real time is simple to operate when used, and the embryo or cell culture environment cannot be changed when the cell or embryo is observed in the culture process; the position of the optical assembly can be adjusted to observe each position of the cell or embryo in the incubator at any time.
Description
Technical Field
The invention belongs to the technical field of cell culture devices, and particularly relates to a constant-temperature culture device capable of being observed in real time.
Background
In the case of cell or embryo culture, it is necessary to observe the growth at any time, to record data or to adjust the culture protocol for culturing the desired cells or embryos. In the prior art, when cells are observed by a microscope, the constant temperature culture device for observing the cells under the microscope moves relatively roughly, so that the cells cannot be clearly observed in detail, the observation precision is reduced, and the constant temperature culture device is not beneficial to the operation of culturing the cells or embryos. In addition, the cell or embryo culture dish needs to be observed at a certain temperature, otherwise, the observation effect and the survival rate of the cells or embryos are influenced. Therefore, the constant temperature culture device for observing cells or embryos, which is convenient for adjusting the observation position and ensuring the temperature of the cell or embryo culture dish during observation, has great practical value.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a constant-temperature culture device capable of being observed in real time.
In order to achieve the above object, the present invention provides the following technical solutions.
The utility model provides a but real-time supervision's constant temperature culture apparatus, includes that the observation platform is adjusted to the three-dimensional, installs at inside three-dimensional guiding mechanism and the optical assembly of observation platform is adjusted to the three-dimensional, and the optical assembly is located three-dimensional guiding mechanism's below to and install the constant temperature embryo incubator of observation platform upper end is adjusted to the three-dimensional.
As a preferable technical scheme of the constant-temperature culture device capable of realizing real-time observation, the constant-temperature embryo incubator comprises an incubator bottom plate arranged at the upper end of a three-dimensional adjusting observation platform, a culture dish arranged at the central position of the upper surface of the incubator bottom plate, a heating plate arranged on the outer side of the culture dish, a heat conduction ring arranged on the upper surface of the heating plate, a heat preservation ring arranged between the culture dish and the heat conduction ring, an incubator heat preservation cover arranged on the upper surface of the incubator bottom plate, a sealing ring arranged between the incubator bottom plate and the incubator heat preservation cover, and an incubator heat preservation cover hood arranged outside the heating plate and the culture dish.
As a preferable technical scheme of the constant temperature culture device capable of observing in real time, the three-way adjusting observation platform comprises an observation platform base and an incubator placing platform which are arranged in parallel, supporting columns which are arranged between the observation platform base and the incubator placing platform through screws, sucking disc supporting legs which are arranged at four corners of the lower surface of the observation platform base, and a transparent observation window which is arranged at the center of the incubator placing platform, wherein the transparent observation window is arranged on the lower surface of the bottom plate of the incubator.
As a preferable technical scheme of the constant temperature culture device capable of observing in real time, the three-way adjusting mechanism comprises a Z-direction sliding table movably arranged in the three-way adjusting observation table, and a Z-direction adjusting track, a Z-direction sliding block, a coarse adjusting knob, a fine adjusting knob, a Z-direction adjusting sliding table connecting frame, a linear bearing, a guide rod, a light source bracket connecting frame, an X-direction stepping motor, an X-direction gear, an X-direction rack, an X-direction guide rail, an X-direction sliding block, a Y-direction rack, a Y-direction guide rail and a Y-direction sliding block, wherein the Z-direction adjusting track, the Z-direction sliding block, the coarse adjusting knob, the fine adjusting knob, the Z-direction adjusting sliding table connecting frame, the linear bearing, the guide rod, the light source bracket connecting frame, the X-direction stepping motor, the X-direction gear, the X-direction rack, the X-direction guide rail, the X-direction sliding block, the Y-direction rack, the Y-direction guide rail and the Y-direction sliding block are arranged on the upper surface of the Z-direction sliding table.
As a preferred technical scheme of the constant temperature culture device capable of observing in real time, the optical assembly comprises an optical assembly sliding table arranged on the upper surface of a Z-direction sliding table, and a Y-direction stepping motor and an objective lens rotary table motor which are arranged on the surface of the optical assembly sliding table, a Y-direction gear is fixed on an output shaft of the Y-direction stepping motor, a rotary table driving gear is fixed on an output shaft of the objective lens rotary table motor, a rotary table driven gear is rotatably arranged on the upper surface of the optical assembly sliding table and meshed with the rotary table driving gear, an objective lens rotary table is fixed on the rotary table driven gear through bolts, a first objective lens, a second objective lens and a third objective lens are arranged on the surface of the objective lens rotary table, an optical path channel mounting frame is fixed on the optical assembly sliding table through bolts, an optical path channel is fixed on the optical path channel mounting frame through an optical path channel fixing ring, one end of the optical path channel, which is close to the objective lens rotary table, is provided with an objective lens imaging receiving port, the other end of the optical path channel is provided with an eyepiece lens observation port, and the objective lens rotary table is provided with an objective image outlet port relative to the lower surfaces of the relative positions of the first objective lens, the second objective lens and the third objective lens.
As a preferable technical scheme of the constant-temperature culture device capable of being observed in real time, an air inlet throttle valve and an air outlet throttle valve are symmetrically arranged on the upper surface of a heat preservation cover of the incubator, the air inlet throttle valve is connected with an air inlet pipe, the air outlet throttle valve is connected with an air outlet pipe, a heating film is arranged inside the heating plate, and a positive power line and a negative power line are mounted on the surface of the heating film.
As a preferable technical scheme of the constant-temperature culture device capable of observing in real time, the end part of the optical assembly sliding table is fixedly connected with a light source support connecting frame, a light source support is arranged on the upper surface of the light source support connecting frame, a light source is fixedly arranged at the upper end of the light source support through a light source height adjusting frame, and the light source is connected with a light source power wire.
Compared with the prior art, the constant-temperature culture device capable of being observed in real time is simple to operate when used, and the embryo or cell culture environment cannot be changed when the cell or embryo is observed in the culture process; the position of the optical assembly can be adjusted to observe each position of the cell or embryo in the incubator at any time.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings.
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a schematic perspective view of the present invention.
Fig. 3 is a schematic front view structure of the present invention.
FIG. 4 is a schematic view of the structure of the constant temperature embryo incubator of the present invention.
FIG. 5 is a schematic view of the internal structure of the incubator of the present invention.
FIG. 6 is a schematic view of the whole section structure of the constant temperature embryo incubator of the present invention.
Fig. 7 is a schematic structural view of a three-way adjusting mechanism in the present invention.
Fig. 8 is a left side view structural schematic diagram of the three-way adjusting mechanism of the present invention.
Fig. 9 is a back schematic view of the three-way adjusting mechanism of the present invention.
Fig. 10 is a front isometric view of an optical assembly in the present invention.
Fig. 11 is a side isometric view of an optical assembly in the present invention.
Fig. 12 is a left side view of the optical assembly of the present invention.
In the figure: 1. a constant temperature embryo incubator; 11. a bottom plate of the incubator; 12. an incubator insulation cover; 13. heating plates; 14. a culture dish; 15. a seal ring; 16. a heat conducting ring; 17. a heat preservation ring; 18. an intake throttle valve; 19. an air outlet throttle valve; 110. an air inlet pipe; 111. an air outlet pipe; 112. a positive power supply line; 113. a negative power supply line; 114. heating the film; 2. a three-way adjusting observation platform; 21. an observation platform base; 22. a sucker support leg; 23. a support pillar; 24. an incubator placing table; 25. a transparent viewing window; 26. adjusting the track in the Z direction; 27. a Z-direction slider; 28. a coarse adjustment knob; 29. fine adjustment of the knob; 210. a Z-direction adjusting sliding table connecting frame; 211. a light source holder; 212. a light source height adjusting bracket; 213. a light source; 214. a light source power line; 3. a three-way adjusting mechanism; 31. a Z-direction sliding table; 32. a linear bearing; 33. a guide bar; 34. a light source bracket connecting frame; 35. an X-direction stepping motor; 36. an X-direction gear; 37. an X-direction rack; 38. an X-direction guide rail; 39. an X-direction sliding block; 310. a Y-direction rack; 311. a Y-direction guide rail; 312. a Y-direction sliding block; 4. an optical component; 41. an optical assembly slide table; 42. a Y-direction stepping motor; 43. a Y-direction gear; 44. an objective lens turret motor; 45. a turntable driving gear; 46. a turntable driven gear; 47. an objective lens turret; 48. a first objective lens; 49. a second objective lens; 410. a third objective lens; 411. an optical path channel; 412. a light path channel fixing ring; 413. an objective lens imaging receiving port; 414. an eyepiece viewing port; 415. a light path channel mounting rack; 416. the object image goes out the image mouth.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
Referring to fig. 1-12, the present invention provides a technical solution: the utility model provides a but real-time supervision's constant temperature culture apparatus, includes that the three-dimensional adjusts observation platform 2, installs at the three-dimensional inside three-dimensional guiding mechanism 3 and the optical assembly 4 of adjusting observation platform 2, and optical assembly 4 is located the below of three-dimensional guiding mechanism 3 to and install the constant temperature embryo incubator 1 of adjusting observation platform 2 upper end at the three-dimensional.
Specifically, the constant temperature embryo incubator 1 includes the incubator bottom plate 11 installed on the upper end of the three-way adjusting observation platform 2, and the culture dish 14 installed at the center position of the upper surface of the incubator bottom plate 11, the heating plate 13 arranged on the outer side of the culture dish 14, the heat conduction ring 16 arranged on the upper surface of the heating plate 13, and the heat insulation ring 17 arranged between the culture dish 14 and the heat conduction ring 16, the incubator heat insulation cover 12 arranged on the upper surface of the incubator bottom plate 11, and the sealing ring 15 arranged between the incubator bottom plate 11 and the incubator heat insulation cover 12, and the incubator heat insulation cover 12 covers the outer parts of the heating plate 13 and the culture dish 14.
Specifically, the three-way adjusting observation platform 2 comprises an observation platform base 21 and an incubator placing platform 24 which are arranged in parallel, supporting columns 23 which are installed between the observation platform base 21 and the incubator placing platform 24 through screws, sucking disc support legs 22 which are installed at four corners of the lower surface of the observation platform base 21, a transparent observation window 25 which is arranged at the central position of the incubator placing platform 24, and the transparent observation window 25 is positioned on the lower surface of the incubator bottom plate 11.
Specifically, the three-way adjusting mechanism 3 includes a Z-direction sliding table 31 movably mounted inside the three-way adjusting observation table 2, and a Z-direction adjusting track 26, a Z-direction slider 27, a coarse adjusting knob 28, a fine adjusting knob 29, a Z-direction adjusting sliding table connecting frame 210, a linear bearing 32, a guide rod 33, a light source support connecting frame 34, an X-direction stepping motor 35, an X-direction gear 36, an X-direction rack 37, an X-direction rail 38, an X-direction slider 39, a Y-direction rack 310, a Y-direction rail 311, and a Y-direction slider 312, which are mounted on the upper surface of the Z-direction sliding table 31 and used for adjusting the positions of the optical assembly 4 and the light source support connecting frame 34.
Specifically, the optical assembly 4 includes an optical assembly sliding table 41 installed on the upper surface of the Z-direction sliding table 31, and a Y-direction stepping motor 42 and an objective lens turntable motor 44 installed on the surface of the optical assembly sliding table 41, a Y-direction gear 43 is fixed on an output shaft of the Y-direction stepping motor 42, a turntable driving gear 45 is fixed on an output shaft of the objective lens turntable motor 44, a turntable driven gear 46 is rotatably installed on the upper surface of the optical assembly sliding table 41, the turntable driven gear 46 is engaged with the turntable driving gear 45, an objective lens turntable 47 is fixed on the turntable driven gear 46 through a bolt, a first objective lens 48, a second objective lens 49 and a third objective lens 410 are installed on the surface of the objective lens turntable 47, an optical path mounting bracket 415 is fixed on the optical assembly sliding table 41 through a bolt, an optical path objective lens 411 is fixed on the optical path mounting bracket 415 through an optical path fixing ring 412, and an imaging receiving port 413 is arranged at one end of the optical path 411 close to the objective lens turntable 47, the other end of the optical path 411 is provided with an eyepiece observation port 414, and the lower surface of the objective turret 47 at a position opposed to the first objective lens 48, the second objective lens 49, and the third objective lens 410 is provided with an object image outlet 416.
Specifically, an air inlet throttle valve 18 and an air outlet throttle valve 19 are symmetrically arranged on the upper surface of the incubator heat-insulating cover 12, the air inlet throttle valve 18 is connected with an air inlet pipe 110, the air outlet throttle valve 19 is connected with an air outlet pipe 111, a heating film 114 is arranged inside the heating plate 13, and a positive power line 112 and a negative power line 113 are arranged on the surface of the heating film 114.
Specifically, the end of the optical assembly sliding table 41 is fixedly connected to the light source support connecting frame 34, the light source support 211 is mounted on the upper surface of the light source support connecting frame 34, the light source 213 is fixedly mounted at the upper end of the light source support 211 through the light source height adjusting frame 212, and the light source 213 is connected to the light source power line 214.
The observation platform base 21 is adsorbed on the table top of the experiment table through the sucker support legs 22, so that shaking during work can be prevented, the incubator placing table 24 is supported on the observation platform base 21 through the support columns 23, and the center of the incubator placing table 24 is provided with a transparent observation window 25 for observing the constant-temperature embryo incubator 1; a three-way adjusting mechanism 3 is arranged at the center of the observation platform base 21 and the center of the incubator placing platform 24; the Z-direction sliding table 31 at the bottom of the three-way adjusting mechanism 3 is fixedly connected with the observation platform base 21 and the incubator placing table 24 through a linear bearing 32 and a guide rod 33; a Z-direction adjusting track 26 is fixedly arranged on the left side of the observation platform base 21 through threads, a Z-direction sliding block 27 capable of sliding in the Z direction is arranged on the Z-direction adjusting track 26, the Z-direction sliding block 27 is fixedly connected with the Z-direction sliding platform 31 through a Z-direction adjusting sliding platform connecting frame 210, and the Z-direction sliding platform 31 can slide on a guide rod 33 through a linear bearing 32 by adjusting a coarse adjusting knob 28 and a fine adjusting knob 29 on the Z-direction sliding block 27; an X-direction rack 37 and an X-direction guide rail 38 are installed on the Z-direction sliding tables 31 through threads, X-direction sliding blocks 39 are arranged on the X-direction guide rail 38, a light source bracket connecting frame 34 is connected on one of the X-direction sliding blocks 39 through threads, a light source bracket 211 and an X-direction stepping motor 35 are installed on the light source bracket connecting frame 34, an X-direction gear 36 is installed on an output shaft of the X-direction stepping motor 35, the X-direction gear 36 is meshed with the X-direction rack 37, Y-direction racks 310 and Y-direction guide rails 311 are connected on the two X-direction sliding blocks 39 through threads, a Y-direction sliding block 312 is installed on the Y-direction guide rail 311, an optical assembly 4 sliding table is installed on the Y-direction sliding block 312, a Y-direction stepping motor 42 is installed on one side of the optical assembly 4 sliding table, a Y-direction gear 43 is installed on an output shaft of the Y-direction stepping motor 42, the Y-direction gear 43 is meshed with the Y-direction rack 310, when the X-direction stepping motor 35 works, the sliding table of the optical component 4 can slide in the X direction, and when the stepping motor 42 in the Y direction works, the sliding table of the optical component 4 can slide in the Y direction; an objective lens turntable 47 is arranged at the center of the sliding table of the optical assembly 4 through threads, and three objective lenses with different magnification factors, an objective lens and an objective lens are arranged on the objective lens turntable 47; an objective lens turntable motor 44 is arranged on the other side of the sliding table of the optical assembly 4, a turntable driving gear 45 is arranged on an output shaft of the objective lens turntable motor 44, a turntable driven gear 46 is fixedly connected below an objective lens turntable 47 through threads, the turntable driving gear 45 is meshed with the turntable driven gear 46, and when the objective lens turntable motor 44 works, the objective lens turntable 47 can rotate, so that the function of automatically switching objective lenses with different multiples is realized; a mounting rack of a light path channel 411 is fixedly mounted on a sliding table of the optical component 4 through threads, an objective imaging receiving port 413 on the light path channel 411 is over against an object image outlet 416 right below an objective which is used on the objective turntable 47, the light path channel 411 is fixedly connected with the mounting rack of the light path channel 411 through a fixing ring of the light path channel 411 through threads, a lens component for imaging is arranged in the light path channel 411, and the lens component reflects an object image received from the objective imaging receiving port 413 to an eyepiece observing port 414 for observation; a light source height adjusting frame 212 is arranged on the light source bracket 211, the light source 213 is arranged on the light source height adjusting frame 212, and the height of the light source 213 can be adjusted by adjusting a thread structure on the light source height adjusting frame 212; a heating plate 13 is arranged in the center of the bottom plate 11 of the incubator, a heating film 114 is arranged in the center of the heating plate 13, a positive power line 112 and a negative power line 113 are arranged on the heating film 114 and used for being connected with a power supply, and a heat conduction ring 16 and a heat preservation ring 17 which are made of heat conduction and heat preservation materials are sequentially filled in the center of the heating plate 13; a sealing ring 15 is pasted in a closed gap between the bottom plate 11 of the incubator and the incubator heat-insulating cover, when the incubator is used, the culture dish 14 is placed in the heat-insulating ring 17, and the incubator heat-insulating cover is buckled on the bottom plate 11 of the incubator and is used for keeping the gas environment inside the constant-temperature embryo incubator 1; an air inlet throttle valve 18 and an air outlet throttle valve 19 are arranged on the incubator heat preservation cover, are respectively communicated with an air inlet pipe 110 and an air outlet pipe 111 and are used for communicating carbon dioxide gas for manufacturing an embryo culture environment.
The working principle and the using process of the invention are as follows: the three-way adjusting observation platform 2 is stably adsorbed on the table surface of the experiment table through the sucker support legs 22 and is connected with a power supply; stably placing the bottom plate 11 of the incubator 1 with the embryos placed on the incubator placing table 24, communicating carbon dioxide gas and switching on a power supply, and at the moment, carrying out a normal culture stage on the embryos in the incubator 1; when the embryo state needs to be observed, the objective lens turntable motor 44 is driven through a computer program, so that the objective lens turntable 47 rotates, an objective lens with a proper multiple is selected, the X-direction stepping motor 35 and the Y-direction stepping motor 42 are driven to adjust the horizontal position of the sliding table of the optical assembly 4, so that the objective lens is aligned to the bottom of the culture dish 14, the coarse adjusting knob 28 and the fine adjusting knob 29 on the Z-direction sliding block 27 are manually adjusted, the height of the Z-direction sliding table 31 is changed, the objective lens is focused, the height of the light source 213 is changed by manually adjusting the light source height adjusting frame 212, and the imaging brightness is adjusted; and finally viewed through an eyepiece viewing port 414.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The utility model provides a but constant temperature culture apparatus of real-time observation which characterized in that: including three-way regulation observation platform (2), install three-way guiding mechanism (3) and optical component (4) inside three-way regulation observation platform (2), and optical component (4) are located the below of three-way guiding mechanism (3) to and install constant temperature embryo incubator (1) of adjusting observation platform (2) upper end at the three-way.
2. The constant-temperature culture device capable of being observed in real time according to claim 1, wherein: constant temperature embryo incubator (1) is including installing culture box bottom plate (11) of adjusting observation platform (2) upper end at the three-dimensional, and install culture dish (14) of upper surface central point department of putting on culture box bottom plate (11), set up hot plate (13) in culture dish (14) outside, and heat conduction ring (16) of setting at hot plate (13) upper surface, and heat preservation ring (17) of setting between culture dish (14) and heat conduction ring (16), set up culture box heat preservation lid (12) at culture box bottom plate (11) upper surface, and install sealing washer (15) between culture box bottom plate (11) and culture box heat preservation lid (12), and culture box heat preservation lid (12) cover the outside at hot plate (13) and culture dish (14).
3. The constant-temperature culture device capable of being observed in real time according to claim 2, wherein: three-way regulation observation platform (2) place platform (24) including parallel arrangement's observation platform base (21) and incubator to and support column (23) between observation platform base (21) and incubator are placed platform (24) through the mounting screw, install sucking disc stabilizer blade (22) at four corners of observation platform base (21) lower surface, set up transparent observation window (25) of placing platform (24) central point department at the incubator, and transparent observation window (25) are located the lower surface of incubator bottom plate (11).
4. The constant-temperature culture apparatus capable of being observed in real time according to claim 3, wherein: three-way guiding mechanism (3) are including movable mounting in inside Z of three-way regulation observation platform (2) to slip table (31), and Z is used for adjusting Z of optical component (4) and light source support link (34) position to adjusting track (26), Z to slider (27), coarse adjustment knob (28), fine adjustment knob (29), Z is to adjusting slip table link (210), linear bearing (32), guide bar (33), light source support link (34), X is to step motor (35), X is to gear (36), X is to rack (37), X is to guide rail (38), X is to slider (39), Y is to rack (310), Y is to guide rail (311) and Y is to slider (312) to slip table (31) upper surface mounting.
5. The isothermal culture device capable of being observed in real time according to claim 4, wherein: optical assembly (4) including installing at optical assembly slip table (41) of Z to slip table (31) upper surface to and optical assembly slip table (41) surface mounting's Y to step motor (42) and objective revolving stage motor (44), Y is fixed with Y to gear (43) to the output shaft of step motor (42), the output shaft of objective revolving stage motor (44) is fixed with revolving stage driving gear (45), the last surface rotation of optical assembly slip table (41) installs revolving stage driven gear (46), and revolving stage driven gear (46) and revolving stage driving gear (45) meshing, revolving stage driven gear (46) are through the bolt fastening have objective revolving stage (47), and the surface mounting of objective revolving stage (47) has first objective (48), second objective (49) and third objective (410), optical assembly slip table (41) are through the bolt fastening have light path passageway mounting bracket (415), the optical path channel mounting frame (415) is fixed with an optical path channel (411) through an optical path channel fixing ring (412), one end, close to the objective lens rotary table (47), of the optical path channel (411) is provided with an objective lens imaging receiving port (413), the other end of the optical path channel (411) is provided with an eyepiece observation port (414), and the objective lens rotary table (47) is provided with an object image outlet (416) relative to the lower surfaces of the relative positions of the first objective lens (48), the second objective lens (49) and the third objective lens (410).
6. The isothermal culture device capable of being observed in real time according to claim 5, wherein: the incubator heat preservation cover is characterized in that an air inlet throttle valve (18) and an air outlet throttle valve (19) are symmetrically arranged on the upper surface of the incubator heat preservation cover (12), the air inlet throttle valve (18) is connected with an air inlet pipe (110), the air outlet throttle valve (19) is connected with an air outlet pipe (111), a heating film (114) is arranged inside the heating plate (13), and a positive power line (112) and a negative power line (113) are arranged on the surface of the heating film (114).
7. The isothermal culture device capable of being observed in real time according to claim 6, wherein: the tip and the light source support link (34) fixed connection of optical assembly slip table (41), the last surface mounting of light source support link (34) has light source support (211), there is light source (213) light source height-adjusting bracket (212) fixed mounting in the upper end of light source support (211), light source (213) are connected with light source power cord (214).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114736800A (en) * | 2022-04-18 | 2022-07-12 | 哈尔滨医科大学 | Embryo incubator with built-in microscopic imaging system |
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| JP2009175754A (en) * | 2009-04-27 | 2009-08-06 | Nikon Corp | Microscope device |
| KR20160035625A (en) * | 2014-09-23 | 2016-04-01 | 전남대학교산학협력단 | Cell observable incubator system |
| CN209923355U (en) * | 2018-12-14 | 2020-01-10 | 李斌 | Real-time monitoring device for living cell culture |
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| CN214830313U (en) * | 2020-12-31 | 2021-11-23 | 北京大学 | Double-box reverse shooting micro-culture device |
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