CN113322168A - Food microorganism detection device and detection method thereof - Google Patents
Food microorganism detection device and detection method thereof Download PDFInfo
- Publication number
- CN113322168A CN113322168A CN202110592460.8A CN202110592460A CN113322168A CN 113322168 A CN113322168 A CN 113322168A CN 202110592460 A CN202110592460 A CN 202110592460A CN 113322168 A CN113322168 A CN 113322168A
- Authority
- CN
- China
- Prior art keywords
- fixed
- shell
- food
- culture dish
- hole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Mechanical Engineering (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of food microorganism detection, in particular to a food microorganism detection device and a detection method thereof, wherein the food microorganism detection device comprises a shell, wherein a rotating disc is rotatably connected to the inner wall of the shell, a plurality of first mounting holes are formed in the surface of the rotating disc in a circumferential array manner, and culture dishes are inserted into the first mounting holes; a feeding cylinder is fixed on the top surface of the shell, a feeding hole is formed in the top surface of the feeding cylinder, a sealing plug is hermetically connected inside the feeding hole, a through hole for feeding a culture dish is formed in the bottom surface of the feeding cylinder, and an intermittent feeding mechanism for intermittently opening and closing the through hole is arranged below the through hole; two ventilation boxes are symmetrically fixed on the side wall of the shell, the ventilation boxes are communicated with the inside of the shell, and a disinfection mechanism for disinfecting one side of the joint is arranged at the joint of the ventilation boxes and the shell; according to the invention, the air entering the shell is disinfected through the disinfection mechanism, so that external microorganisms are prevented from entering the shell, and detection of microorganisms in broken food is facilitated.
Description
Technical Field
The invention relates to the field of food microorganism detection, in particular to a food microorganism detection device and a detection method thereof.
Background
The food microorganism detection is a detection method which uses the theory and method of microbiology to detect the type, quantity, property and influence of the microorganism in the food on the human health so as to judge whether the food meets the quality standard.
The patent document who discloses some relevant food microorganism detection among the prior art, chinese patent with application number 202011293080.6 discloses a food microorganism detection device, including compound box, the fixed cover in one side at compound box top has connect clamping module mechanism, clamping module mechanism's inside activity has cup jointed preparation module mechanism. The invention integrates and installs a preparation module mechanism, a power module mechanism, a disinfection module mechanism and a microorganism detection and culture module by taking the composite box body as a main carrier.
In the prior art, when microorganisms in food are detected, a plurality of groups of samples need to be collected, then the samples are grouped and put into a detection device for culture, and finally, other indexes such as the total number of bacterial colonies, coliform groups, pathogenic bacteria and the like are observed to judge whether the food meets the quality standard or not.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a food microorganism detection device and a detection method thereof.
In order to achieve the above purposes, the technical scheme adopted by the invention is as follows: a food microorganism detection device comprises a shell, wherein a plurality of supporting pads are fixed on the bottom surface of the shell, a rotating disc is rotatably connected on the inner wall of the shell, a rotating shaft is rotatably connected in the middle of the rotating disc, the top of the rotating shaft penetrates through the top surface of the shell and extends out to be fixed with a first motor through a coupling, and the first motor is fixed on the top surface of the shell; a plurality of first mounting holes are formed in the surface of the rotating disc in a circumferential array, culture dishes are inserted into the first mounting holes, an opening for enabling the culture dishes to enter and exit is formed in the top surface of the shell, an entering mechanism for enabling the culture dishes to hermetically enter and exit the shell is arranged in the opening, and a moving-out mechanism is fixedly connected to the bottom of the inner wall of the shell at a position corresponding to the opening; a feeding cylinder is fixed on the top surface of the shell, a feeding hole is formed in the top surface of the feeding cylinder, a sealing plug is hermetically connected inside the feeding hole, a through hole for feeding a culture dish is formed in the bottom surface of the feeding cylinder, and an intermittent feeding mechanism for intermittently opening and closing the through hole is arranged below the through hole; two ventilation boxes are symmetrically fixed on the side wall of the shell, a ventilation groove communicated with the outside is formed in the bottom surface of each ventilation box, the ventilation boxes are communicated with the inside of the shell, and a disinfection mechanism used for disinfecting the space on one side of the joint is arranged at the joint of each ventilation box and the shell; when the food sterilization device works, firstly, a culture dish is placed into the opening, the culture dish is conveyed into a first mounting hole on the rotating disc through the entering mechanism, then, an electric power supply is switched on, the rotating shaft is driven to rotate through the output shaft of the motor, the rotating disc is driven to rotate through the rotating shaft, so that the culture dish rotates on the rotating disc, food raw materials are placed into the feeding cylinder, the food raw materials can be crushed through the crushing mechanism, the crushed food is sequentially added into each culture dish through the intermittent feeding mechanism, before the culture dish is added, the environment in the shell is sterilized through the sterilizing mechanism by light rays, after the culture dish is added, the sterilizing direction is switched, the air in the ventilating box is sterilized, microorganisms in the external air are prevented from entering the shell, and the mixing of the external microorganisms and the microorganisms in the food is caused, in addition, in the whole process, the food sample injection and the culture of the bacterial colony on the food sample are carried out under the condition of no microbial interference in the external environment, the culture environment is kept excellent, and the detection of the microbes in the broken food is facilitated.
Preferably, a crushing mechanism for crushing the food raw materials is arranged in the feeding cylinder, the crushing mechanism comprises a second motor, the second motor is fixed on the top surface of the feeding cylinder, the tail end of an output shaft of the second motor penetrates through the top surface of the feeding cylinder and extends into the feeding cylinder, a crushing rod is fixed on the tail end of the output shaft of the second motor, and a plurality of crushing columns are fixed on a side arm of the crushing rod; during operation, inside putting into a feeding section of thick bamboo with food raw materials, then, switch on the power of second motor, it rotates to drive broken pole and broken post through the second motor, thereby carry out the breakage to the food raw materials of feeding section of thick bamboo inside, need not the manual work and carry out the breakage, convenient and fast, and a feeding section of thick bamboo sets up inside the casing, with outside air contactless, inside the culture dish was added through intermittent type feeding mechanism directly to food after the breakage, the in-process is not contacted with outside microorganism, thereby avoid outside microorganism to mix with the microorganism in the food, cause and detect inaccurate condition and take place, be favorable to detecting the microorganism in the food after the breakage.
Preferably, the intermittent feeding mechanism comprises a connecting sleeve and a rotating plate, the connecting sleeve is fixed at the edge of the bottom surface of the feeding cylinder, and a movable rod is inserted in the connecting sleeve and comprises an extrusion end and a rebound end, a second spring is fixed at the rebounding end of the movable rod and is fixed on the side wall of the shell, the top surface of the rebound end of the movable rod is in sliding seal with the through hole, the top surface of the movable rod close to the rebound end is provided with a discharge chute, the rotating plate is sleeved and fixed on the rotating shaft, a plurality of arc-shaped protruding blocks are fixed on the side wall of the rotating plate in a circumferential array, the number of the arc-shaped protruding blocks is the same as that of the first mounting holes, when one of the first mounting holes is positioned right below the through hole, the arc-shaped protruding block is used for extruding the extruding end of the movable rod to drive the discharge chute to be communicated with the through hole; when the culture dish is in operation, the rotating plate and the culture dish are driven to synchronously rotate through the rotating shaft, the top surface of the rebounding end of the movable plate and the through hole are in a sealing state in an initial state, when the culture dish rotates to be under the through hole, the end part of the arc-shaped protruding block extrudes the extrusion end of the movable rod to drive the discharge chute to be communicated with the through hole, so that food in the feeding cylinder passes through the through hole and the discharge chute to enter the culture dish, the culture dish continues to rotate, the end part of the arc-shaped protruding block gradually separates from the movable rod, the extrusion force on the movable rod is gradually reduced, the second spring pushes the movable rod under the action of elastic force to reset the movable rod, so that the discharge chute gradually separates from the through hole, the flow rate of the food is reduced, the top surface of the rebounding end of the movable plate is sealed with the through hole, when the next culture dish moves to be under the through hole, the subsequent operation is repeated, and the food raw materials can be sequentially added into each culture dish, and the food is not contacted with external microorganisms in the feeding process, so that the condition of inaccurate detection caused by mixing of the external microorganisms with the microorganisms in the food is avoided, and the detection of the microorganisms in the broken food is facilitated.
Preferably, a vibration mechanism for driving the culture dish to vibrate is arranged below the rotating plate, the vibration mechanism comprises an elastic strip, a plurality of arc strips and a plurality of protruding balls, the protruding balls are fixed on the bottom surface of the culture dish, the elastic strip is fixed on the inner wall of the shell below the first mounting hole and is in contact with the protruding balls, the arc strips are placed inside the first mounting hole, a third spring is placed between the arc strips and the first mounting hole, one end of the third spring is fixed on the arc strips, and the other end of the third spring is fixed on the inner wall of the first mounting hole; when the culture dish is in collision with the elastic strip, the culture dish is driven to move in the first mounting hole, and the arc-shaped strip and the third spring are pushed by the culture dish to enable the third spring to generate elasticity; when the convex ball is separated from the elastic strip, the third spring pushes the culture dish to move towards the opposite direction; through a plurality of protruding balls in the striking of elastic strip to make the culture dish vibrate in first mounting hole inside, the inside food of culture dish is along with the culture dish vibrations and distribute each position on the culture dish bottom surface, is convenient for make the inside food evenly distributed of culture dish, is favorable to the reproduction of microorganism in the food, is favorable to detecting the microorganism in the food.
Preferably, the disinfection mechanism comprises rotating strips, the rotating strips are sequentially arranged at the joint of the shell and the ventilation box from top to bottom, two ends of each rotating strip are rotatably connected to the inner wall of the ventilation box, a plurality of traction ropes are jointly connected and fixed between the rotating strips, a disinfection lamp is fixedly connected to the top surface of each rotating strip, and an adjusting mechanism for adjusting the joint rotation of the rotating strips is arranged at the bottom of each rotating strip; during operation, in the prior art, the detection device is communicated with the outside, bacteria in the outside air easily enter the detection device and enter the sample, and the detection of the microorganisms in the sample is not facilitated, the technical scheme can solve the above problems, and the specific working mode is as follows, before the culture dish is added, after the broken food sample is injected into the culture dish, the disinfection direction is switched, the shell is communicated with the outside air through the ventilation box, so that the outside oxygen can enter the shell, the growth of the microorganisms in the food sample inside the shell is facilitated, the air entering the shell is disinfected through the disinfection lamp, the microorganisms in the food sample are prevented from contacting with the microorganisms in the outside air, and the condition that the detection is inaccurate due to the mixing of the outside microorganisms and the microorganisms in the food is avoided, is beneficial to detecting the microorganisms in the broken food.
Preferably, the adjusting mechanism comprises a connecting rod and a pressure plate, the top of the connecting rod is connected to a rotating strip at the bottom through a connecting rope, the bottom of the connecting rod penetrates through the air box and extends into the shell, a lifting block is fixed on the bottom of the connecting rod, a groove is formed in the side wall of the lifting block, the pressure plate is placed above the rotating disc, the edge of the pressure plate is connected to the inside of the groove in a sliding mode, a plurality of second mounting holes are formed in the top surface of the pressure plate and located right above the first mounting holes, a plurality of fourth springs are placed between the pressure plate and the rotating disc, the top ends of the fourth springs are fixed to the bottom surface of the pressure plate, and the bottom ends of the fourth springs are fixed to the top surface of the rotating disc; when the device works, when all culture dishes are placed in the first mounting hole and the second mounting hole, the pressure plate is pressed downwards by the weight of the culture dishes under the action of gravity, the pressure plate is inserted in the groove, the lifting block is pressed downwards by the pressure plate, the lifting block downwards pulls one end of the rotating strip at the bottom through the connecting rod, one end of the rotating strip at the bottom is rotated downwards, all the rotating strips synchronously rotate under the action of the guy wire rope, the sterilizing lamp on the rotating strip irradiates the inside of the ventilating box by adjusting the angle of the rotating strip, the air in the ventilating box is sterilized, the microorganisms in the gas entering the shell are killed, the microorganisms in a food sample are prevented from contacting with the microorganisms in the outside air, the mixing of the outside microorganisms and the microorganisms in the food is avoided, and the condition of inaccurate detection is caused, the detection of microorganisms in the crushed food is facilitated; and after taking out the culture dish, the pressure disk upwards moves under the elastic force effect of fourth spring, promotes the promotion piece rebound, promotes the one end that the rotation strip was upwards promoted through the connecting rod to the promotion piece, makes the sterilamp on the rotation strip shine inside the casing, kills the microorganism of casing inside, prevents to detect that the microorganism in the food remains inside the casing, influences the food detection quality next time.
Preferably, the entry mechanism comprises an installation cylinder and a guide cylinder, the guide cylinder is fixed at the edge below the opening, the installation cylinder is fixed at the edge above the opening, an inlet and an outlet are formed in the side wall of the installation cylinder, a fifth spring is fixed on the top surface in the installation cylinder, a piston is fixed at the bottom of the fifth spring, the piston is sealed in the guide cylinder in a sliding manner, a pull rod is fixed at the top of the piston, and a pull plate is fixed at the top end of the pull rod, which penetrates through the installation cylinder and extends out; when the culture dish is required to be placed, the pulling plate is pulled upwards, the pulling plate pulls the piston upwards through the pull rod, the piston is separated from the opening, the culture dish is convenient to insert into the opening and falls onto the pressure plate along the guide cylinder, the pressure plate is rotated, the culture dish is limited through the guide cylinder, and when the second mounting hole moves to the position under the culture dish, the culture dish automatically falls into the second mounting hole, so that the operation is convenient and rapid; and after putting into the casing with the culture dish inside, the piston continues to move down under the elasticity effect of fifth spring, seals the opening, realizes instant sealed, prevents that too much unsterilized outside air from getting into inside the casing, is favorable to making inside the casing keep aseptic environment.
Preferably, the moving-out mechanism comprises an electric push rod, the electric push rod is placed under the guide cylinder, the bottom of the electric push rod is fixed on the inner wall of the shell, a top plate is fixed at the top of the electric push rod, and a yielding hole is formed in the top surface of the top plate; the during operation, when needs shift out the culture dish, through switch-on electric putter's power, piston rod through electric putter upwards promotes the roof, upwards promote the culture dish through the roof, the hole of stepping down has been seted up on the roof, make the protruding ball embedding of culture dish bottom step down downthehole portion, prevent promoting the in-process, the condition that the culture dish took place to empty takes place, be favorable to making the culture dish steadily rise, and finally, the culture dish moves up along guide cylinder, and jack-up piston upwards, pass the opening after, can follow exit and take out, be convenient for wash the culture dish.
A method for detecting food microorganisms comprises the following steps:
the method comprises the following steps that firstly, the interior of a shell is continuously disinfected through a disinfection mechanism, so that the interior of the shell is kept in an aseptic environment;
placing the culture dish into the shell through the inlet mechanism, starting the adjusting mechanism through the gravity of the culture dish, turning over the disinfecting mechanism through the adjusting mechanism, disinfecting the inside of the ventilation box, and disinfecting the air entering the shell;
step three, putting food raw materials into the feeding cylinder, and crushing the food raw materials through a crushing mechanism;
step four, adding the crushed food into each culture dish in sequence through an intermittent feeding mechanism, and vibrating the culture dishes through a vibrating mechanism to uniformly distribute the food on the inner bottom surfaces of the culture dishes;
and step five, moving the culture dish out of the shell by starting the moving-out mechanism, and detecting the microorganisms in the culture dish by an external microscope device.
Compared with the prior art, the invention has the following beneficial effects:
one, inside putting into a feeding section of thick bamboo with food raw materials, it rotates to drive broken pole and broken post through the second motor, thereby carry out the breakage to the food raw materials of a feeding section of thick bamboo inside, need not manual operation, convenient and fast, and a feeding section of thick bamboo sets up inside the casing, inside food after the breakage directly adds the culture dish through intermittent type feeding mechanism, the in-process does not contact with outside microorganism, thereby avoid the microorganism in outside microorganism and the food to mix, cause and detect the emergence of unsafe condition, be favorable to detecting the microorganism in the food after the breakage.
Second, drive the rotating plate and culture dish to rotate synchronously through the axis of rotation, under the initial condition, the top surface of the rebound end of the movable plate and the through hole are in a sealed state, when the culture dish rotates under the through hole, the end of the arc-shaped bulge piece extrudes the extrusion end of the movable rod to drive the discharge chute to be communicated with the through hole, so that the food in the feeding cylinder passes through the through hole and the discharge chute to enter the culture dish, the culture dish continues to rotate, the end of the arc-shaped bulge piece gradually separates from the movable rod, the extrusion force to the movable rod is gradually reduced, the second spring pushes the movable rod under the action of the elastic force to reset the movable rod, so that the discharge chute gradually separates from the through hole, the flow rate of the food is reduced, until the top surface of the rebound end of the movable plate is sealed with the through hole, when the next culture dish moves under the through hole, the continuous operation is repeated, so that the food raw materials can be sequentially added into each culture dish, and the food is not contacted with external microorganisms in the feeding process, so that the condition of inaccurate detection caused by mixing of the external microorganisms with the microorganisms in the food is avoided, and the detection of the microorganisms in the broken food is facilitated.
Thirdly, the culture dish is driven to rotate through the rotating plate, after feeding is completed, the culture dish moves to one side of the elastic strip along with the rotating plate, the convex ball at the bottom of the culture dish is in collision contact with the elastic strip, when the convex ball collides with the elastic strip, the culture dish is driven to move in the first mounting hole, and the arc-shaped strip and the third spring are pushed through the culture dish, so that the third spring generates elasticity; when the convex ball is separated from the elastic strip, the third spring pushes the culture dish to move towards the opposite direction; through a plurality of protruding balls in the striking of elastic strip to make the culture dish vibrate in first mounting hole inside, the inside food of culture dish is along with the culture dish vibrations and distribute each position on the culture dish bottom surface, is convenient for make the inside food evenly distributed of culture dish, is favorable to the reproduction of microorganism in the food, is favorable to detecting the microorganism in the food.
Fourthly, when all the culture dishes are placed in the first mounting hole and the second mounting hole, the pressure plate is pressed downwards by the weight of the culture dishes under the action of gravity, because the pressure plate is inserted in the groove, the lifting block is pressed downwards by the pressure plate, the lifting block pulls one end of the rotating strip at the bottom downwards by the connecting rod to ensure that one end of the rotating strip at the bottom downwards rotates, under the action of the wire pulling rope, all the rotating strips synchronously rotate, and the sterilizing lamps on the rotating strips irradiate the inside of the ventilation box by adjusting the angles of the rotating strips, the air in the ventilation box is disinfected, the microorganisms in the gas entering the shell are killed, the microorganisms in the food sample are prevented from contacting with the microorganisms in the outside air, therefore, the condition that the detection is inaccurate due to the mixing of external microorganisms and microorganisms in the food is avoided, and the detection of the microorganisms in the broken food is facilitated; and after taking out the culture dish, the pressure disk upwards moves under the elastic force effect of fourth spring, promotes the promotion piece rebound, promotes the one end that the rotation strip was upwards promoted through the connecting rod to the promotion piece, makes the sterilamp on the rotation strip shine inside the casing, kills the microorganism of casing inside, prevents to detect that the microorganism in the food remains inside the casing, influences the food detection quality next time.
Drawings
FIG. 1 is a schematic flow diagram of the process of the present invention;
FIG. 2 is a schematic view of the overall structure of the present invention;
FIG. 3 is a schematic cross-sectional rear view of the integrated ventilation box of the present invention;
FIG. 4 is a schematic diagram of the structure at A in FIG. 3 according to the present invention;
FIG. 5 is a schematic diagram of the structure at B in FIG. 3 according to the present invention;
FIG. 6 is a schematic structural view of the integral feed cylinder of the present invention taken along the rear section thereof;
FIG. 7 is a schematic view of the structure of FIG. 6 at C in accordance with the present invention;
FIG. 8 is a schematic view of the gap feed mechanism of the present invention;
FIG. 9 is a cross-sectional rear view of the electric putter according to the present invention;
FIG. 10 is a schematic view of a rotating disk structure of the present invention;
FIG. 11 is a schematic view of a platen configuration of the present invention;
fig. 12 is a cross-sectional rear structure view of the integral guide cylinder of the present invention.
In the figure: the device comprises a shell 1, a supporting pad 2, a rotating disc 3, a rotating shaft 4, a first motor 5, a first mounting hole 6, a culture dish 7, an opening 8, a feeding cylinder 9, a feeding hole 10, a sealing plug 11, a through hole 12, a ventilation box 13, a ventilation groove 14, a second motor 15, a crushing rod 16, a crushing column 17, a connecting sleeve 18, a rotating plate 19, a movable rod 20, a second spring 21, a discharging groove 22, an arc-shaped protruding block 23, an elastic strip 24, an arc-shaped strip 25, a protruding ball 26, a third spring 27, a rotating strip 28, a traction rope 29, a disinfection lamp 30, a connecting rod 31, a pressure plate 32, a lifting block 33, a groove 34, a second mounting hole 35, a fourth spring 36, a mounting cylinder 37, a guide cylinder 38, an inlet and outlet 39, a fifth spring 40, a piston 41, a pull rod 42, a pulling plate 43, an electric push rod 44, a top plate 45 and a abdicating hole 46.
Detailed Description
The following description is presented to disclose the invention so as to enable any person skilled in the art to practice the invention. The preferred embodiments in the following description are given by way of example only, and other obvious variations will occur to those skilled in the art.
As shown in fig. 1-12, a food microorganism detection device comprises a housing 1, wherein a plurality of support pads 2 are fixed on the bottom surface of the housing 1, a rotating disc 3 is rotatably connected on the inner wall of the housing 1, a rotating shaft 4 is rotatably connected in the middle of the rotating disc 3, the top of the rotating shaft 4 penetrates through the top surface of the housing 1 and extends out to be fixed with a first motor 5 through a coupling, and the first motor 5 is fixed on the top surface of the housing 1; a plurality of first mounting holes 6 are circumferentially arranged on the surface of the rotating disc 3 in an array manner, culture dishes 7 are inserted into the first mounting holes 6, an opening 8 for enabling the culture dishes 7 to enter and exit is formed in the top surface of the shell 1, an entering mechanism for enabling the culture dishes 7 to hermetically enter and exit the shell 1 is arranged in the opening 8, and a moving-out mechanism is fixedly connected to the position, corresponding to the opening 8, of the bottom of the inner wall of the shell 1; a feeding cylinder 9 is fixed on the top surface of the shell 1, a feeding hole 10 is formed in the top surface of the feeding cylinder 9, a sealing plug 11 is hermetically connected inside the feeding hole 10, a through hole 12 for feeding the culture dish 7 is formed in the bottom surface of the feeding cylinder 9, and an intermittent feeding mechanism for intermittently opening and closing the through hole 12 is arranged below the through hole 12; two ventilation boxes 13 are symmetrically fixed on the side wall of the shell 1, a ventilation groove 14 communicated with the outside is formed in the bottom surface of each ventilation box 13, each ventilation box 13 is communicated with the inside of the shell 1, and a disinfection mechanism used for disinfecting the space on one side of the joint is arranged at the joint of each ventilation box 13 and the shell 1; during operation, in the prior art, the detection device is communicated with the outside, bacteria in the outside air easily enter the detection device and enter a sample, and the detection of microorganisms in the sample is not facilitated, the technical scheme can solve the problems, and the specific working mode is as follows, firstly, the culture dish is placed in the opening 8, the culture dish is conveyed to the inside of the first mounting hole 6 on the rotating disc 3 through the entering mechanism, then, the power supply of the motor 5 is switched on, the rotating shaft 4 is driven to rotate through the output shaft of the motor 5, the rotating disc 3 is driven to rotate through the rotating shaft 4, so that the culture dish 7 rotates on the rotating disc 3, food raw materials are placed in the feeding cylinder 9, the food raw materials can be crushed through the crushing mechanism, and the crushed food is sequentially added into each culture dish 7 through the intermittent feeding mechanism, before the culture dish 7 is added, carry out light disinfection and sterilization through the environment of disinfection mechanism in the casing 1 earlier, add the back when culture dish 7, switch the disinfection direction, disinfect to the inside air of ventilative case 13, prevent that the microorganism in the outside air from getting into inside the casing 1, cause the condition that the microorganism in outside microorganism and the food mixes to take place, and in the whole process, food sample injection and to go up the cultivation of bacterial colony and all go on under the condition of little biological disturbance in no external environment to food sample, keep the good of cultivateing the environment, be favorable to detecting the microorganism in the food after the breakage.
As a further embodiment of the present invention, a crushing mechanism for crushing food raw materials is arranged inside the feeding cylinder 9, the crushing mechanism includes a second motor 15, the second motor 15 is fixed on the top surface of the feeding cylinder 9, the tail end of the output shaft of the second motor 15 penetrates through the top surface of the feeding cylinder 9 and extends into the feeding cylinder 9, and then a crushing rod 16 is fixed on the tail end of the output shaft, and a plurality of crushing columns 17 are fixed on the side arms of the crushing rod 16; when the food processor works, in the prior art, when a food sample is made, a plurality of instruments are needed, and in the process of transferring food, microorganisms in the air easily enter the food, so that the microorganism detection of the food is inaccurate, the technical scheme can solve the problems, and the specific working mode is as follows, the food raw materials are put into the feeding cylinder 9, then the power supply of the second motor 15 is switched on, the second motor 15 drives the crushing rod 16 and the crushing column 17 to rotate, so that the food raw materials in the feeding cylinder 9 are crushed without manual crushing, the feeding cylinder 9 is arranged in the shell 1 and is not in contact with the external air, the crushed food is directly added into the culture dish 7 through the intermittent feeding mechanism and is not in contact with the external microorganisms in the process, so that the situation that the external microorganisms are mixed with the microorganisms in the food, and the detection is inaccurate is caused, is beneficial to detecting the microorganisms in the broken food.
As a further embodiment of the invention, the intermittent feeding mechanism comprises a connecting sleeve 18 and a rotating plate 19, the connecting sleeve 18 is fixed at the edge of the bottom surface of the feeding cylinder 9, a movable rod 20 is inserted in the connecting sleeve 18, the movable rod 20 comprises a squeezing end and a rebounding end, the rebounding end of the movable rod 20 is fixed with a second spring 21, the second spring 21 is fixed on the side wall of the shell 1, the top surface of the rebounding end of the movable rod 20 is in sliding seal with the through hole 12, a discharge chute 22 is arranged on the top surface of the movable rod 20 close to the rebounding end, the rotating plate 19 is sleeved and fixed on the rotating shaft 4, a plurality of arc-shaped raised blocks 23 are fixed on the side wall of the rotating plate 19 in a circumferential array, the number of the arc-shaped raised blocks 23 is the same as that of the first mounting holes 6, when one of the first mounting holes 6 is positioned right below the through hole 12, the arc-shaped raised blocks 23 are used for squeezing the squeezing end of the movable rod 20, the discharge chute 22 is driven to be communicated with the through hole 12; during operation, in the prior art, when a food sample is produced, a plurality of instruments are needed, during the process of transferring food, microorganisms in the air easily enter the food, and the accuracy of detecting the microorganisms in the food is affected, the technical scheme can solve the above problems, and the specific working mode is as follows, the rotating plate 19 and the culture dish 7 are driven to synchronously rotate through the rotating shaft 4, under the initial state, the top surface of the rebounding end of the movable plate 20 and the through hole 12 are in a sealing state, when the culture dish 7 rotates to be right under the through hole 12, the end part of the arc-shaped raised block 23 extrudes the extruding end of the movable rod 20 to drive the discharging groove 22 to be communicated with the through hole 12, so that the food in the feeding cylinder 9 passes through the through hole 12 and the discharging groove 22 to enter the culture dish 7, the culture dish 7 continues to rotate, the end part of the arc-shaped raised block 23 gradually breaks away from the movable rod 20, and the extruding force on the movable rod 20 is gradually reduced, second spring 21 is under the elastic force effect, promote movable rod 20, make movable rod 20 reset, thereby make blown down tank 22 break away from through-hole 12 gradually, make the food velocity of flow slow down, it is sealed with through-hole 12 until the resilience end top surface of fly leaf 20, when next culture dish 7 moves to through-hole 12 below, repeated continuation of the operation, then can realize adding food raw materials to every culture dish 7 inside in proper order, and food does not contact with outside microorganism at reinforced in-process, thereby avoid the microorganism in outside microorganism and the food to mix, cause the inaccurate condition of detection to take place, be favorable to detecting the microorganism in the food after the breakage.
As a further embodiment of the present invention, a vibration mechanism for driving the culture dish 7 to vibrate is disposed below the rotating plate 19, the vibration mechanism includes an elastic strip 24, a plurality of arc strips 25 and a plurality of protruding balls 26, the protruding balls 26 are fixed on the bottom surface of the culture dish 7, the elastic strip 24 is fixed on the inner wall of the housing 1 below the first mounting hole 6, and the elastic strip 24 is in contact with the protruding balls 26, the arc strips 25 are placed inside the first mounting hole 6, and a third spring 27 is placed between the arc strips 25 and the first mounting hole 6, one end of the third spring 27 is fixed on the arc strips 25, and the other end of the third spring 27 is fixed on the inner wall of the first mounting hole 6; during operation, food is added into the culture dish 7 and is easy to be accumulated together, and cannot be rapidly dispersed and distributed in the culture dish 7, so that the proliferation of microorganisms in the food is not facilitated, the technical scheme can solve the problems, and the specific working mode is as follows, the culture dish 7 is driven to rotate by the rotating plate 19, after the feeding is completed, the culture dish 7 moves towards one side of the elastic strip 24 along with the rotating plate 19, the convex ball 26 at the bottom of the culture dish 7 is in collision contact with the elastic strip 24, when the convex ball 26 collides with the elastic strip 24, the culture dish 7 is driven to move in the first mounting hole 6, and the arc strip 25 and the third spring 27 are pushed by the culture dish 7, so that the third spring 27 generates elasticity; when the convex ball 26 is disengaged from the elastic strip 24, the third spring 27 pushes the culture dish 7 to move in the opposite direction; through a plurality of protruding balls 26 in the striking of elastic strip 24 to make culture dish 7 at the inside vibrations of first mounting hole 6, the inside food of culture dish 7 shakes and distributes each position on culture dish 7 bottom surface along with culture dish 7, is convenient for make the inside food evenly distributed of culture dish 7, is favorable to the reproduction of microorganism in the food.
As a further embodiment of the invention, the sterilizing mechanism comprises rotating strips 28, the rotating strips 28 are sequentially arranged at the joint of the shell 1 and the ventilation box 13 from top to bottom, two ends of each rotating strip 28 are rotatably connected to the inner wall of the ventilation box 13, a plurality of traction ropes 29 are jointly connected and fixed between the rotating strips 28, a sterilizing lamp 30 is fixedly connected to the top surface of each rotating strip 28, and an adjusting mechanism for adjusting the joint rotation of the rotating strips 28 is arranged at the bottom of each rotating strip 28; during operation, in the prior art, the detection device is communicated with the outside, bacteria in the outside air easily enter the detection device and enter the sample, which is not beneficial to detecting the microorganisms in the sample, the technical scheme can solve the above problems, the specific working mode is as follows, before the culture dish 7 is added, after the broken food sample is injected into the culture dish 7, the disinfection direction is switched, the shell 2 is communicated with the outside air through the ventilation box 13, so that the outside oxygen can enter the shell 1, which is beneficial to the growth of the microorganisms in the food sample inside the shell 1, and the air entering the shell 1 is disinfected through the disinfection lamp 30, so as to prevent the microorganisms in the food sample from contacting with the microorganisms in the outside air, thereby avoiding the mixing of the outside microorganisms and the microorganisms in the food and causing the occurrence of inaccurate detection, is beneficial to detecting the microorganisms in the broken food.
As a further embodiment of the present invention, the adjusting mechanism includes a connecting rod 31 and a pressure plate 32, the top of the connecting rod 31 is connected to the rotating bar 28 at the bottom through a connecting rope, the bottom of the connecting rod 31 penetrates through the air box 13 and extends into the interior of the housing 1, and then a lifting block 33 is fixed, a groove 34 is formed on the side wall of the lifting block 33, the pressure plate 32 is placed above the rotating disc 3, and the edge of the pressure plate 32 is slidably connected to the interior of the groove 34, a plurality of second mounting holes 35 are formed on the top surface of the pressure plate 32, the second mounting holes 35 are located right above the first mounting holes 6, a plurality of fourth springs 36 are placed between the pressure plate 32 and the rotating disc 3, the top ends of the fourth springs 36 are fixed on the bottom surface of the pressure plate 32, and the bottom ends of the fourth springs 36 are fixed on the top surface of the rotating disc 3; in operation, when all the culture dishes 7 are placed in the first and second mounting holes 6 and 35, the pressure plate 32 is pressed downwards by the weight of the culture dishes 7 under the action of gravity, the pressure plate 32 is inserted in the groove 34, so that the lifting block 33 is pressed downwards by the pressure plate 32, the lifting block 33 pulls one end of the rotating strip 28 at the bottom downwards through the connecting rod 31, one end of the rotating strip 28 at the bottom downwards rotates downwards, all the rotating strips 28 synchronously rotate under the action of the wire pulling rope 29, the sterilizing lamp 30 on the rotating strip 28 irradiates the inside of the air permeable box 13 by adjusting the angle of the rotating strip 28, the air inside the air permeable box 13 is sterilized, the microorganisms in the air entering the shell 1 are killed, the microorganisms in the food sample are prevented from contacting with the microorganisms in the outside air, and the mixing of the outside microorganisms with the microorganisms in the food is avoided, the condition of inaccurate detection is caused, and the detection of microorganisms in the crushed food is facilitated; and after taking out the culture dish, pressure disk 32 under the elastic force effect of fourth spring 36, upward movement promotes piece 33 upward movement, promotes piece 33 and upwards promotes the one end of rotating strip 28 through connecting rod 31, makes the disinfection lamp on the rotating strip 38 shine inside the casing 1, kills the microorganism of casing 1 inside, prevents to detect that the microorganism in the food remains inside casing 1, influences the food detection quality next time.
As a further embodiment of the present invention, the entry mechanism includes a mounting cylinder 37 and a guiding cylinder 38, the guiding cylinder 38 is fixed at the lower edge of the opening 8, the mounting cylinder 37 is fixed at the upper edge of the opening 8, the side wall of the mounting cylinder 37 is provided with an inlet and outlet 39, a fifth spring 40 is fixed on the inner top surface of the mounting cylinder 37, a piston 41 is fixed at the bottom of the fifth spring 40, the piston 41 is slidably sealed inside the guiding cylinder 38, a pull rod 42 is fixed at the top of the piston 41, and a pull plate 43 is fixed at the top end of the pull rod which penetrates through the mounting cylinder 37 and extends out; when the culture dish 7 needs to be placed in the working process, the pulling plate 43 is pulled upwards, the pulling plate 43 pulls the piston 41 upwards through the pull rod 42, the piston 41 is separated from the opening 8, the culture dish 7 is convenient to insert into the opening 8 and falls onto the upper side of the pressure plate 32 along the guide cylinder 38, the pressure plate 32 is rotated, the culture dish 7 is limited through the guide cylinder 38, when the second mounting hole 35 moves to the position right below the culture dish 7, the culture dish 7 automatically falls into the second mounting hole 35, and the operation is convenient and rapid; and after putting culture dish 7 into casing 1 inside, piston 41 continues to move down under the elastic force of fifth spring 40, seals opening 8, realizes instant sealing, prevents that too much unsterile outside air from getting into inside casing 1, is favorable to making the inside aseptic environment that keeps of casing 1.
As a further embodiment of the present invention, the removing mechanism comprises an electric push rod 44, the electric push rod 44 is placed under the guide cylinder 38, the bottom of the electric push rod 44 is fixed on the inner wall of the housing 1, the top of the electric push rod 44 is fixed with a top plate 45, and the top surface of the top plate 45 is provided with a relief hole 46; the during operation, when culture dish 7 is shifted out to needs, power through switch-on electric putter 44, piston rod through electric putter 44 upwards promotes roof 45, upwards promote culture dish 7 through roof 45, set up the hole of stepping down 46 on the roof 45, make inside the protruding ball 26 embedding hole of stepping down 46 of culture dish 7 bottom, prevent at the promotion in-process, the condition that culture dish 7 takes place to empty takes place, be favorable to making culture dish 7 steadily rise, and finally, culture dish 7 moves upwards along guide tube 38, and upwards jack-up piston 41, pass opening 8 after, can follow exit 39 and take out, be convenient for wash culture dish 7.
A method for detecting food microorganisms comprises the following steps:
step one, continuously disinfecting the interior of the shell 1 through a disinfection mechanism to keep the interior of the shell 1 in an aseptic environment;
placing the culture dish 7 into the shell 1 through the entering mechanism, starting the adjusting mechanism through the gravity of the culture dish 7, turning over the disinfecting mechanism through the adjusting mechanism, disinfecting the inside of the ventilation box 13, and disinfecting the air entering the shell 1;
step three, putting food raw materials into the feeding cylinder 9, and crushing the food raw materials through a crushing mechanism;
step four, adding the crushed food into each culture dish 7 in sequence through an intermittent feeding mechanism, and vibrating the culture dishes 7 through a vibrating mechanism to uniformly distribute the food on the inner bottom surfaces of the culture dishes 7;
and step five, moving the culture dish 7 out of the shell 1 by starting the moving-out mechanism, and detecting the microorganisms in the culture dish 7 by an external microscope device.
The working principle of the invention is as follows:
firstly, putting a culture dish into the opening 8, conveying the culture dish into a first mounting hole 6 on a rotating disc 3 through an entering mechanism, then switching on a power supply of an electric motor 5, driving a rotating shaft 4 to rotate through an output shaft of the motor 5, driving the rotating disc 3 to rotate through the rotating shaft 4, so that the culture dish 7 rotates on the rotating disc 3, putting food raw materials into a feeding cylinder 9, crushing the food raw materials through a crushing mechanism, sequentially adding the crushed food into each culture dish 7 through an intermittent feeding mechanism, performing light sterilization and disinfection on the environment in the shell 1 through a disinfection mechanism before the culture dish 7 is added, switching the disinfection direction after adding the culture dish 7, disinfecting the air in the ventilation box 13, preventing microorganisms in the outside air from entering the shell 1, and causing the mixing of the outside microorganisms and the microorganisms in the food, in addition, in the whole process, the food sample injection and the culture of the bacterial colony on the food sample are carried out under the condition of no microbial interference in the external environment, the culture environment is kept excellent, and the detection of the microbes in the broken food is facilitated.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined by the appended claims and their equivalents.
Claims (9)
1. A food microorganism detection device, includes casing (1), its characterized in that: a plurality of supporting pads (2) are fixed on the bottom surface of the shell (1), a rotating disc (3) is rotatably connected on the inner wall of the shell (1), a rotating shaft (4) is rotatably connected in the middle of the rotating disc (3), the top of the rotating shaft (4) penetrates through the top surface of the shell (1) and extends out to be fixed with a first motor (5) through a coupler, and the first motor (5) is fixed on the top surface of the shell (1);
a plurality of first mounting holes (6) are formed in the surface of the rotating disc (3) in a circumferential array, culture dishes (7) are inserted into the first mounting holes (6), an opening (8) for enabling the culture dishes (7) to enter and exit is formed in the top surface of the shell (1), an entering mechanism for enabling the culture dishes (7) to enter and exit the shell (1) in a sealing mode is arranged in the opening (8), and a moving-out mechanism is fixedly connected to the position, corresponding to the opening (8), of the bottom of the inner wall of the shell (1);
a feeding cylinder (9) is fixed on the top surface of the shell (1), a feeding hole (10) is formed in the top surface of the feeding cylinder (9), a sealing plug (11) is hermetically connected in the feeding hole (10), a through hole (12) for feeding a culture dish (7) is formed in the bottom surface of the feeding cylinder (9), and an intermittent feeding mechanism for intermittently opening and closing the through hole (12) is arranged below the through hole (12);
two ventilation boxes (13) are symmetrically fixed on the side wall of the shell (1), a ventilation groove (14) communicated with the outside is formed in the bottom surface of each ventilation box (13), the ventilation boxes (13) are communicated with the inside of the shell (1), and a disinfection mechanism used for disinfecting the space on one side of the joint is arranged at the joint of each ventilation box (13) and the shell (1).
2. The food microorganism detection apparatus according to claim 1, wherein: the feeding cylinder (9) is internally provided with a crushing mechanism for crushing food raw materials, the crushing mechanism comprises a second motor (15), the second motor (15) is fixed on the top surface of the feeding cylinder (9), the tail end of an output shaft of the second motor (15) penetrates through the top surface of the feeding cylinder (9) and extends into the feeding cylinder (9), a crushing rod (16) is fixed behind the inner part of the feeding cylinder (9), and a plurality of crushing columns (17) are fixed on side arms of the crushing rod (16).
3. The food microorganism detection apparatus according to claim 1, wherein: the intermittent feeding mechanism comprises a connecting sleeve (18) and a rotating plate (19), the connecting sleeve (18) is fixed at the edge of the bottom surface of a feeding cylinder (9), a movable rod (20) is inserted in the connecting sleeve (18), the movable rod (20) comprises an extrusion end and a rebounding end, the rebounding end of the movable rod (20) is fixed with a second spring (21), the second spring (21) is fixed on the side wall of a shell (1), the top surface of the rebounding end of the movable rod (20) is in sliding seal with a through hole (12), a discharge chute (22) is formed in the top surface, close to the rebounding end, of the movable rod (20), the rotating plate (19) is sleeved and fixed on a rotating shaft (4), a plurality of arc-shaped protruding blocks (23) are fixed on the side wall of the rotating plate (19) in a circumferential array manner, and the number of the arc-shaped protruding blocks (23) is the same as that of the first mounting holes (6), when one of the first mounting holes (6) is positioned under the through hole (12), the arc-shaped protruding block (23) is used for extruding the extruding end of the movable rod (20) to drive the discharge chute (22) to be communicated with the through hole (12).
4. The food microorganism detection apparatus according to claim 3, wherein: the utility model discloses a culture dish (7) vibration device, including rotor plate (19), deflector (19) below is provided with and is used for driving about the vibrations mechanism of culture dish (7), vibrations mechanism includes elasticity strip (24), a plurality of arc strip (25) and a plurality of protruding ball (26), protruding ball (26) are fixed on the bottom surface of culture dish (7), elasticity strip (24) are fixed on casing (1) inner wall of first mounting hole (6) below, and elasticity strip (24) contact with protruding ball (26), arc strip (25) are placed inside first mounting hole (6), and third spring (27) have been placed between arc strip (25) and first mounting hole (6), the one end of third spring (27) is fixed on arc strip (25), the other end of third spring (27) is fixed on first mounting hole (6) inner wall.
5. The food microorganism detection apparatus according to claim 1, wherein: disinfection mechanism is including rotating strip (28), rotate strip (28) and arrange the junction at casing (1) and ventilative case (13) from top to bottom in proper order, it all rotates on the inner wall of ventilative case (13) to rotate strip (28) both ends, it is fixed with a plurality of haulage ropes (29) to rotate to connect jointly between strip (28), every rotate equal fixedly connected with sterilamp (30) on strip (28) top surface, it is provided with the adjustment mechanism who is used for adjusting rotation strip (28) common pivoted to rotate strip (28) bottom to rotate.
6. The food microorganism detection apparatus according to claim 5, wherein: the adjusting mechanism comprises a connecting rod (31) and a pressure plate (32), the top of the connecting rod (31) is connected to a rotating strip (28) at the bottom through a connecting rope, the bottom of the connecting rod (31) penetrates through an air box (13) and extends into the shell (1) to be fixed with a lifting block (33), a groove (34) is formed in the side wall of the lifting block (33), the pressure plate (32) is placed above the rotating disc (3), the edge of the pressure plate (32) is slidably connected into the groove (34), a plurality of second mounting holes (35) are formed in the top surface of the pressure plate (32), the second mounting holes (35) are located right above the first mounting holes (6), a plurality of fourth springs (36) are placed between the pressure plate (32) and the rotating disc (3), and the top ends of the fourth springs (36) are fixed to the bottom surface of the pressure plate (32), the bottom end of the fourth spring (36) is fixed on the top surface of the rotating disc (3).
7. The food microorganism detection apparatus according to claim 1, wherein: the entering mechanism comprises an installation cylinder (37) and a guide cylinder (38), the guide cylinder (38) is fixed at the edge below the opening (8), the installation cylinder (37) is fixed at the edge above the opening (8), an inlet and an outlet (39) are formed in the side wall of the installation cylinder (37), a fifth spring (40) is fixed on the inner top surface of the installation cylinder (37), a piston (41) is fixed at the bottom of the fifth spring (40), the piston (41) is in sliding sealing inside the guide cylinder (38), a pull rod (42) is fixed at the top of the piston (41), and a pull plate (43) is fixed at the top end of the pull rod in a penetrating mode on the installation cylinder (37) and extending out.
8. The food microorganism detection apparatus according to claim 7, wherein: the removing mechanism comprises an electric push rod (44), the electric push rod (44) is placed under the guide cylinder (38), the bottom of the electric push rod (44) is fixed on the inner wall of the shell (1), a top plate (45) is fixed at the top of the electric push rod (44), and a yielding hole (46) is formed in the top surface of the top plate (45).
9. A method for detecting microorganisms in food, which is applied to the apparatus for detecting microorganisms in food according to any one of claims 1 to 8, comprising: the detection method comprises the following steps:
the method comprises the following steps that firstly, the interior of a shell (1) is continuously disinfected through a disinfection mechanism, so that the interior of the shell (1) is kept in a sterile environment;
placing the culture dish (7) into the shell (1) through the entering mechanism, starting the adjusting mechanism through the gravity of the culture dish (7), turning over the disinfecting mechanism through the adjusting mechanism, disinfecting the inside of the breathable box (13), and disinfecting the air entering the shell (1);
step three, putting food raw materials into the feeding cylinder (9), and crushing the food raw materials through a crushing mechanism;
step four, adding the crushed food into each culture dish (7) in sequence through an intermittent feeding mechanism, and vibrating the culture dishes (7) through a vibrating mechanism to uniformly distribute the food on the inner bottom surfaces of the culture dishes (7);
and fifthly, moving the culture dish (7) out of the shell (1) by starting the moving-out mechanism, and detecting the microorganisms in the culture dish (7) through an external microscope device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110592460.8A CN113322168A (en) | 2021-05-28 | 2021-05-28 | Food microorganism detection device and detection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110592460.8A CN113322168A (en) | 2021-05-28 | 2021-05-28 | Food microorganism detection device and detection method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113322168A true CN113322168A (en) | 2021-08-31 |
Family
ID=77422204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110592460.8A Pending CN113322168A (en) | 2021-05-28 | 2021-05-28 | Food microorganism detection device and detection method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113322168A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164097A (en) * | 2021-12-08 | 2022-03-11 | 陈科 | Food detection device convenient to microorganism culture |
| CN115109706A (en) * | 2022-06-09 | 2022-09-27 | 王登英 | Culture process for microbial detection |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201984683U (en) * | 2011-04-06 | 2011-09-21 | 山东师范大学附属中学 | Microorganism continuous cultivation experimental apparatus for teaching |
| US20150010996A1 (en) * | 2012-02-29 | 2015-01-08 | Jtec Corporation | Cell culture device having culture medium replacement function |
| KR20160091733A (en) * | 2015-01-26 | 2016-08-03 | 제주특별자치도(제주특별자치도해양수산연구원장) | Automatic Feeding Apparatus for Rotifers |
| CN205443316U (en) * | 2016-03-31 | 2016-08-10 | 杨兰花 | Food is incubator for biological detection a little |
| US20170145470A1 (en) * | 2015-11-24 | 2017-05-25 | Fahad Mohammedsaleh ALBUKHARI | Specimen transfer apparatus and method |
| CN206396220U (en) * | 2017-01-25 | 2017-08-11 | 新疆医科大学附属肿瘤医院 | A kind of electric heating constant temperature culture apparatus suitable for cell research |
| CN108441422A (en) * | 2018-04-08 | 2018-08-24 | 郭庆 | A kind of soil bacteria screening system |
| CN108704230A (en) * | 2018-06-02 | 2018-10-26 | 张伟萍 | A kind of structural fire protection multi-function emergency fire hydrant cabinet |
| CN109321449A (en) * | 2018-10-09 | 2019-02-12 | 郑州韦尔特生物科技有限公司 | A kind of microbial cultivation device |
| CN208964930U (en) * | 2018-09-20 | 2019-06-11 | 襄阳小欧农业开发有限公司 | A kind of incubator for Microbiological detection of foods |
| CN211318395U (en) * | 2019-11-28 | 2020-08-21 | 庐山市绿游生态农业开发有限公司 | Multifunctional food detection equipment |
| CN111849751A (en) * | 2020-07-02 | 2020-10-30 | 宁波卫生职业技术学院 | Microorganism culture device and operation method |
| CN112391276A (en) * | 2020-11-18 | 2021-02-23 | 邓文朝 | Food microorganism detection device |
| WO2021035455A1 (en) * | 2019-08-26 | 2021-03-04 | 山东英才学院 | Pressure steam sterilizer for children's clothing |
-
2021
- 2021-05-28 CN CN202110592460.8A patent/CN113322168A/en active Pending
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201984683U (en) * | 2011-04-06 | 2011-09-21 | 山东师范大学附属中学 | Microorganism continuous cultivation experimental apparatus for teaching |
| US20150010996A1 (en) * | 2012-02-29 | 2015-01-08 | Jtec Corporation | Cell culture device having culture medium replacement function |
| KR20160091733A (en) * | 2015-01-26 | 2016-08-03 | 제주특별자치도(제주특별자치도해양수산연구원장) | Automatic Feeding Apparatus for Rotifers |
| US20170145470A1 (en) * | 2015-11-24 | 2017-05-25 | Fahad Mohammedsaleh ALBUKHARI | Specimen transfer apparatus and method |
| CN205443316U (en) * | 2016-03-31 | 2016-08-10 | 杨兰花 | Food is incubator for biological detection a little |
| CN206396220U (en) * | 2017-01-25 | 2017-08-11 | 新疆医科大学附属肿瘤医院 | A kind of electric heating constant temperature culture apparatus suitable for cell research |
| CN108441422A (en) * | 2018-04-08 | 2018-08-24 | 郭庆 | A kind of soil bacteria screening system |
| CN108704230A (en) * | 2018-06-02 | 2018-10-26 | 张伟萍 | A kind of structural fire protection multi-function emergency fire hydrant cabinet |
| CN208964930U (en) * | 2018-09-20 | 2019-06-11 | 襄阳小欧农业开发有限公司 | A kind of incubator for Microbiological detection of foods |
| CN109321449A (en) * | 2018-10-09 | 2019-02-12 | 郑州韦尔特生物科技有限公司 | A kind of microbial cultivation device |
| WO2021035455A1 (en) * | 2019-08-26 | 2021-03-04 | 山东英才学院 | Pressure steam sterilizer for children's clothing |
| CN211318395U (en) * | 2019-11-28 | 2020-08-21 | 庐山市绿游生态农业开发有限公司 | Multifunctional food detection equipment |
| CN111849751A (en) * | 2020-07-02 | 2020-10-30 | 宁波卫生职业技术学院 | Microorganism culture device and operation method |
| CN112391276A (en) * | 2020-11-18 | 2021-02-23 | 邓文朝 | Food microorganism detection device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164097A (en) * | 2021-12-08 | 2022-03-11 | 陈科 | Food detection device convenient to microorganism culture |
| CN114164097B (en) * | 2021-12-08 | 2024-01-09 | 广州源美生物科技发展有限公司 | Food detection device convenient to microorganism is cultivateed |
| CN115109706A (en) * | 2022-06-09 | 2022-09-27 | 王登英 | Culture process for microbial detection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113322168A (en) | Food microorganism detection device and detection method thereof | |
| CN210311934U (en) | Automatic sterilization and disinfection electric garbage can | |
| CN205727536U (en) | One boar is with aseptic raising isolator | |
| CN106474511A (en) | A kind of animal husbandry epidemic disease protection raising farm ground sterilization equipment | |
| CN209882943U (en) | Conveying channel of aquatic product workshop | |
| CN112472845A (en) | Movable degassing unit in sheep hurdle | |
| CN112391276A (en) | Food microorganism detection device | |
| CN112831400B (en) | Solid surface microorganism sampling method | |
| CN209872471U (en) | Recirculating aquaculture system based on biological flocculation ectopic treatment | |
| CN210671639U (en) | Biological fermentation cattle bed for beef cattle breeding | |
| CN220201949U (en) | Cell culture apparatus | |
| CN210580825U (en) | High-voltage pulse electric field sterilizing device for fruits | |
| CN209065778U (en) | A kind of strain adding set for aerobic fermentation turner | |
| CN208454942U (en) | Microbial rapid detection culture apparatus in a kind of food | |
| CN112210472A (en) | Kitchen garbage fermentation deodorization bacterial cultivates equipment | |
| CN110882905A (en) | Vibrating screen for producing soil microbial activator | |
| CN215022914U (en) | Beautiful flower dendrobium's sample degassing unit | |
| CN217038744U (en) | High-temperature sterilization device for food and beverage processing | |
| CN213624144U (en) | Microbial detection is with degassing unit that disinfects | |
| CN219417439U (en) | Detection equipment for water quality luminous bacteria | |
| CN219951001U (en) | Probiotics cultivates equipment | |
| CN211407612U (en) | Fluid feed sterilizer for breeding stichopus japonicus | |
| CN220476615U (en) | Poultry storehouse with disinfection function disinfects | |
| CN215708981U (en) | Intelligent garbage cabinet with wireless communication function | |
| CN212814038U (en) | Sterilizing device for producing food |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |