CN115109706A - Culture process for microbial detection - Google Patents
Culture process for microbial detection Download PDFInfo
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- CN115109706A CN115109706A CN202210646577.4A CN202210646577A CN115109706A CN 115109706 A CN115109706 A CN 115109706A CN 202210646577 A CN202210646577 A CN 202210646577A CN 115109706 A CN115109706 A CN 115109706A
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000001514 detection method Methods 0.000 title claims description 9
- 230000000813 microbial effect Effects 0.000 title description 3
- 244000005700 microbiome Species 0.000 claims abstract description 65
- 239000011248 coating agent Substances 0.000 claims abstract description 43
- 238000000576 coating method Methods 0.000 claims abstract description 43
- 239000000758 substrate Substances 0.000 claims abstract description 39
- 238000003860 storage Methods 0.000 claims abstract description 30
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000005096 rolling process Methods 0.000 description 9
- 239000003973 paint Substances 0.000 description 5
- 230000001737 promoting effect Effects 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000006835 compression Effects 0.000 description 3
- 238000007906 compression Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000026058 directional locomotion Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
Abstract
The invention discloses a culture process for detecting microorganisms, which is characterized by comprising the following operation steps: s1, designing and manufacturing a culture apparatus: s2, preparation: ultraviolet disinfection treatment or heating treatment is carried out on the transparent cover of the culture device through external equipment, so that the pollution of external microorganisms to a culture sample is avoided, and meanwhile, a storage tank and a culture substrate which are prepared in advance are respectively placed on a movable plate and a rotating disc; s3, conveying the sample: the piston push rod moves repeatedly in the suction cylinder, then the suction cylinder sucks the microorganism sample in the storage tank into the suction cylinder through the guide pipe, the microorganism sample is injected into the coating cylinder on the fixing plate through the conveying pipe, the microorganism sample is conveyed onto the culture substrate through the coating cylinder, and the coating cylinder is conveyed onto the culture substrate through the matching of the coating cylinder and all parts; and S4, separating and culturing.
Description
Technical Field
The invention relates to the field of microbial culture, in particular to a culture process for microbial detection.
Background
The microorganism comprises a large group of organisms including bacteria, viruses, fungi, small protozoa and the like, individuals are tiny and closely related to human life, and the microorganism is widely related to various fields such as health, medicine, industry, agriculture and environmental protection.
Disclosure of Invention
The invention aims to provide a culture process for detecting microorganisms, which has the advantages of preventing external microorganism pollution and facilitating the separation and observation of the cultured microorganisms and solves the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a culture process for detecting microorganisms is characterized by comprising the following operation steps:
s1, designing and manufacturing a culture apparatus:
s2, preparation:
ultraviolet disinfection treatment or heating treatment is carried out on the transparent cover (1) of the culture device through external equipment, so that the pollution of external microorganisms to a culture sample is avoided, and meanwhile, a storage tank (7) and a culture substrate (14) which are prepared in advance are respectively placed on a movable plate (12) and a rotating disc (6);
s3, delivering sample:
the device is characterized in that the device repeatedly moves in an suction cylinder (17) through a piston push rod (21), then the suction cylinder (17) sucks a microorganism sample in a storage tank (7) into the suction cylinder (17) through a guide pipe (27), the microorganism sample is injected into a coating cylinder (16) on a fixed plate (15) through a delivery pipe (19), the microorganism sample is delivered onto a culture substrate (14) through the coating cylinder (16), and the coating cylinder (16) is delivered onto the culture substrate (14) through the arrangement of the coating cylinder (16) and the cooperation of various components;
s4, separation and culture:
the sliding strip (3) can be repeatedly moved in the mounting seat (2) by continuously rotating the first gear (10), the fixed plate (15) is enabled to slide in a limiting mode in the through groove in the outer wall of the mounting seat (2), the coating barrel (16) on the fixed plate (15) repeatedly moves and coats a sample on the surface of the culture substrate (14), the movable plate (12) is matched to directionally move on the guide rail (13) to enable the sample to be uniformly distributed on the culture substrate (14) in a non-overlapping mode, microorganisms mixed together originally are separated from the surface of the culture substrate (14) along the scribing part of the coating barrel (16), dispersed single colonies are obtained, pure species are obtained, and the later-stage detection of the microorganisms is facilitated.
In the scheme, the culture device comprises a transparent cover (1) for closed culture, a mounting seat (2) is arranged in the transparent cover (1), two guide rails (13) are fixedly connected to one side of the outer wall of the mounting seat (2), and a movable plate (12) is arranged on each guide rail (13); the movable plate (12) is provided with a through hole and is in limit sliding connection with the guide rail (13) through the through hole, and the upper surface of the movable plate (12) is provided with a culture substrate (14) for culturing microorganisms; an auxiliary device convenient for separating microorganisms is arranged in the mounting seat (2), the auxiliary device comprises a sliding strip (3), and the upper surface of the mounting seat (2) is provided with a sliding groove and is in limit sliding connection with the sliding strip (3) through the sliding groove; the outer wall of the mounting seat (2) is provided with a through groove at one side close to the movable plate (12) and is connected with a fixing plate (15) in a limiting and sliding mode through the through groove, the fixing plate (15) penetrates through the through groove and is fixedly connected with one side of the sliding strip (3), and a coating cylinder (16) for inoculating microorganisms is arranged on the fixing plate (15).
In the scheme, the sliding strip (3) is provided with an installation column (4), the surface of the installation column (4) is provided with a through hole and is fixedly connected with a rotating column (5) through the through hole in a fixed-shaft rotating manner, and the upper surface of the rotating column (5) is fixedly connected with a rotating disc (6); the center of the upper surface of the rotating disc (6) is fixedly connected with a storage tank (7) for culturing microorganisms, a torsion spring (31) is sleeved on the outer contour of the rotating column (5), two ends of the torsion spring (31) are respectively and fixedly connected with the mounting column (4) and the rotating disc (6), and a shaking mechanism for shaking the storage tank (7) is arranged on the mounting seat (2);
the pushing mechanism comprises four connecting columns (8) symmetrically arranged on the edge of the upper surface of the only rotating disc (6), one side, close to the fixed plate (15), of the upper surface of the mounting seat (2) is fixedly connected with a mounting frame (18), a limiting sleeve (20) is arranged on the mounting frame (18), a through groove is formed in the surface of the limiting sleeve (20), and a pushing block (25) matched with the connecting columns (8) is connected in a limiting and sliding mode through the through groove; promote piece (25) be located mounting bracket (18) be provided with lead to groove complex integral key shaft, the integral key shaft outline is located mounting bracket (18) and promotes between piece (25) the cover and is equipped with reset spring (29), it sets up for the slope to promote one side that piece (25) is close to spliced pole (8).
In the present case, mount pad (2) upper surface is provided with the pushing mechanism who promotes slider (3), pushing mechanism is including being located connector (26) of slider (3) upper surface edge one side, fixedly connected with inserts slightly between connector (26) both sides inner wall, insert a little outline cover and be equipped with movable rod (28), the one end that connector (26) were kept away from in movable rod (28) has seted up the through-hole and has rotated through this through-hole and be connected with eccentric post (30), mount pad (2) one side fixedly connected with mounting panel (9), fixed axis rotation carousel (24) of connecting are seted up to mounting panel (9) upper surface, carousel (24) and eccentric post (30) fixed connection.
In the scheme, a flow guide pipe (27) is arranged in the storage tank (7), one end, far away from the connecting column (8), of the flow guide pipe (27) is communicated with a liquid inlet of the suction cylinder (17), and a check valve is arranged between the flow guide pipe (27) and the suction cylinder (17) in a communicated manner; honeycomb duct (27) liquid outlet through connection has conveyer pipe (19), conveyer pipe (19) link up the department with mounting bracket (18) and are provided with the reverse valve, the one end that mounting bracket (18) were kept away from in conveyer pipe (19) is fixed with coating barrel (16) through connection, honeycomb duct (27) and conveyer pipe (19) are the hose.
In the scheme, a through hole is formed in one side, close to a first gear (10), of the surface of the mounting plate (9) and is fixedly and rotatably connected with a second gear (11) through the through hole in a fixed shaft mode, the second gear (11) is meshed with the first gear (10), a screw rod (22) is coaxially and fixedly connected with the surface of the first gear (10), a connecting plate (23) is fixedly connected to one side of the upper surface of the movable plate (12), and a threaded hole is formed in the surface of the connecting plate (23) and is in threaded connection with the screw rod (22) through the threaded hole.
The beneficial effects are as follows:
1. the culture process has the advantages that the pushing block is arranged, the rotating storage tank can be reset under the matching of the torsion spring and each part, the storage tank can shake repeatedly, and samples in the storage tank can be mixed fully;
2. according to the culture process, a microorganism sample is conveyed to a culture substrate through a coating cylinder, and the coating cylinder is conveyed to the culture substrate through the matching of the coating cylinder and each component;
3. the culture process realizes separation of the original mixed microorganisms on the surface of the culture substrate along the scribing part of the coating cylinder by arranging the movable plate and matching the movable plates with the parts to obtain dispersed single bacterial colonies so as to obtain pure strains and facilitate detection of the microorganisms in the later period.
Drawings
FIG. 1 is a process flow diagram of the present invention.
FIG. 2 is a schematic perspective view of a culture apparatus used in the present invention;
FIG. 3 is a first perspective view of a mounting base of the culture apparatus of the present invention;
FIG. 4 is a schematic perspective view of a second embodiment of a mounting base of the culture apparatus of the present invention;
FIG. 5 is a schematic top view of the structure of FIG. 3;
FIG. 6 is a schematic front view of the structure of FIG. 2;
FIG. 7 is an enlarged view of the structure at A in FIG. 3;
FIG. 8 is a schematic perspective view of a push block of the culture apparatus of the present invention.
In the figure: 1. a transparent cover; 2. a mounting seat; 3. a slide bar; 4. mounting a column; 5. rotating the column; 6. rotating the disc; 7. storing the tank; 8. connecting columns; 9. mounting a plate; 10. a first gear; 11. a second gear; 12. a movable plate; 13. a guide rail; 14. culturing a substrate; 15. a fixing plate; 16. a coating cylinder; 17. a suction cylinder; 18. a mounting frame; 19. a delivery pipe; 20. a limiting sleeve; 21. a piston push rod; 22. a screw; 23. a connecting plate; 24. a turntable; 25. a pushing block; 26. a connector; 27. a flow guide pipe; 28. a movable rod; 29. a return spring; 30. an eccentric column; 31. a torsion spring.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Referring to fig. 1 to 8, a cultivation process for detecting microorganisms includes the following steps:
s1, designing and manufacturing a culture apparatus:
example one
Referring to fig. 1 to 7, the culture apparatus for microorganism detection includes a transparent cover 1 for closed culture, a mounting base 2 is disposed in the transparent cover 1, two guide rails 13 are fixedly connected to one side of an outer wall of the mounting base 2, a movable plate 12 is disposed on the guide rails 13, a through hole is disposed on the movable plate 12 and is in limited sliding connection with the guide rails 13 through the through hole, a culture substrate 14 for culturing microorganisms is disposed on an upper surface of the movable plate 12, and an auxiliary device for facilitating separation of the microorganisms is disposed in the mounting base 2.
Auxiliary device includes sliding strip 3, and 2 upper surfaces of mount pad have been seted up the spout and have been offered the spacing sliding connection of spout and sliding strip 3 through this spout, and 2 outer walls of mount pad are close to fly leaf 12 one side and have been offered logical groove and have been led to the spacing sliding connection through this logical groove and have a fixed plate 15, and fixed plate 15 runs through logical groove and with 3 one side fixed connection of sliding strip, be provided with on the fixed plate 15 to inoculate the material coating cylinder 16 to the microorganism. Through setting up translucent cover 1, carry out closed cultivation in translucent cover 1 to cooperate through auxiliary device, inoculate the microorganism to culture substrate 14 through scribbling feed cylinder 16, realize cultivateing the microorganism.
As shown in fig. 2-7, the slide bar 3 is provided with a mounting post 4, a through hole is formed in the surface of the mounting post 4, a rotating post 5 is fixedly connected to the surface of the mounting post 4 through the through hole in a fixed-axis rotating manner, a rotating disc 6 is fixedly connected to the upper surface of the rotating post 5, a storage tank 7 for culturing microorganisms is fixedly connected to the central position of the upper surface of the rotating disc 6, a torsion spring 31 is sleeved on the outer contour of the rotating post 5, and two ends of the torsion spring 31 are respectively fixedly connected to the mounting post 4 and the rotating disc 6.
Put fixedly connected with through the 6 upper surface central points of rolling disc to carry out the holding vessel 7 that cultivates the microorganism, when rolling disc 6 rotates on erection column 4 through rotation post 5, through torsional spring 31 both ends respectively with erection column 4 and 6 fixed connection of rolling disc, can make pivoted holding vessel 7 reset under the effect of torsional spring 31, realize rocking 7 repeatedly holding vessel, can make the sample in the holding vessel 7 carry out the intensive mixing.
As shown in fig. 2-7, be provided with on mount pad 2 and carry out the rocking mechanism that rocks to storage tank 7, pushing mechanism includes four spliced poles 8 that unique rolling disc 6 upper surface edge symmetry set up, one side fixedly connected with mounting bracket 18 that mount pad 2 upper surface is close to fixed plate 15, set up stop collar 20 on the mounting bracket 18, stop collar 20 surface has been seted up logical groove and has been led to the groove and have with spliced pole 8 complex through this logical spacing sliding connection and promote piece 25, it is provided with in the mounting bracket 18 and leads to logical groove complex integral key shaft to promote piece 25 to be located, the integral key shaft outline is located the mounting bracket 18 and promotes and overlaps between the piece 25 and be equipped with reset spring 29, it sets up for the slope to promote one side that piece 25 is close to spliced pole 8.
The spout that the sliding strip 3 was seted up on mount pad 2 carries out spacing slip that relapses, can make spliced pole 8 on the rolling disc 6 and the promotion piece 25 contact on the mounting bracket 18, when spliced pole 8 and the inclined plane of the one side of promoting piece 25 contact, be located the mounting bracket 18 through promoting piece 25 and be provided with the integral key shaft with logical groove, can make and promote piece 25 and carry out endwise slip in mounting bracket 18, when promoting piece 25 and remove to stop collar 20 one side in mounting bracket 18, and make through setting up torsional spring 31 and promote piece 25 and reset, the inclined plane of pushing piece 25 one side promotes spliced pole 8 under the effect of torsional spring 31, make rolling disc 6 with rotation post 5 fixed connection rotate on erection column 4, realize rocking to depositing jar 7 relapse, make the sample in the storage jar 7 carry out intensive mixing.
As shown in fig. 2-7, the upper surface of the mounting base 2 is provided with a pushing mechanism for pushing the sliding strip 3, the pushing mechanism includes a connector 26 located on one side of the edge of the upper surface of the sliding strip 3, a pin is fixedly connected between the inner walls of two sides of the connector 26, an outer contour sleeve of the pin is provided with a movable rod 28, one end of the movable rod 28, which is far away from the connector 26, is provided with a through hole and is rotatably connected with an eccentric column 30 through the through hole, one side of the mounting base 2 is fixedly connected with a mounting plate 9, the upper surface of the mounting plate 9 is provided with a turntable 24 which is rotatably connected with a fixed shaft, the turntable 24 is fixedly connected with the eccentric column 30, one side of the turntable 24, which is far away from the movable rod 28, is fixedly connected with a first gear 10, and an output shaft of the first gear is fixedly connected with an external power source.
Open the outside power supply with gear 10 output shaft fixed connection, set up to driving motor through the outside power supply, through driving motor output shaft and gear 10 pivot fixed connection, drive gear 10 through driving motor and rotate, can make when gear 10 rotates with gear 10 output shaft fixed connection's carousel 24 rotates, be provided with eccentric post 30 on through carousel 24, the cover is equipped with movable rod 28 on 30 outlines through eccentric post, rotate along with carousel 24 when eccentric post 30 can pass through connector 26 on the movable rod 28 pulling sliding strip 3, can make sliding strip 3 carry out the repetitive motion in mount pad 2.
Example two
As shown in fig. 3, 4, 5 and 8, on the basis of the first embodiment, basically the same as the first embodiment, further, a piston push rod 21 is fixedly connected to a side of the spline shaft of the pushing block 25 away from the connecting column 8, a suction cylinder 17 for feeding the paint cartridge 16 is arranged on the mounting frame 18, and a compression chamber matched with the piston push rod 21 is arranged in the suction cylinder 17.
The piston push rod 21 arranged in the suction cylinder 17 moves repeatedly in the suction cylinder 17, so that the suction cylinder 17 can suck the storage tank 7 repeatedly, the suction can be performed conveniently through the vent pipe which is arranged on the storage tank 7 and is convenient for circulation, and the vent pipe is provided with the filter screen to prevent the external environment from polluting the storage tank 7.
As shown in fig. 3-6, a flow guide tube 27 is disposed in the storage tank 7, one end of the flow guide tube 27, which is far away from the connection column 8, is in through connection with a liquid inlet of the suction tube 17, a check valve is disposed in through connection between the flow guide tube 27 and the suction tube 17, a delivery pipe 19 is in through connection with a liquid outlet of the flow guide tube 27, a reverse valve is disposed at a through position between the delivery pipe 19 and the mounting frame 18, one end of the delivery pipe 19, which is far away from the mounting frame 18, is fixed and is in through connection with the coating tube 16, and both the flow guide tube 27 and the delivery pipe 19 are hoses.
The piston push rod 21 moves in the compression cavity of the suction cylinder 17, a one-way valve is arranged at the liquid outlet and the liquid inlet of the guide pipe 27, under the cooperation of the one-way valve, when the piston push rod 21 repeatedly moves in the suction cylinder 17, the suction cylinder 17 sucks a microorganism sample in the storage tank 7 into the suction cylinder 17 through the guide pipe 27, the microorganism sample is injected into the coating cylinder 16 on the fixing plate 15 through the conveying pipe 19, the microorganism sample is conveyed onto the culture substrate 14 through the coating cylinder 16, the coating cylinder 16 is conveyed onto the culture substrate 14 through the cooperation between the coating cylinder 16 and each component, the storage tank 7 and the coating cylinder 16 are both flexible pipes through the arrangement of the guide pipe 27 and the conveying pipe 19, and the storage tank 7 and the coating cylinder 16 are convenient to move.
EXAMPLE III
As shown in fig. 3-6, on the basis of the first and second embodiments, basically the same as the first and second embodiments, further, a through hole is formed on one side of the surface of the mounting plate 9 close to the first gear 10, and a second gear 11 is connected to the through hole in a fixed-axis rotating manner, the second gear 11 is engaged with the first gear 10, a screw 22 is fixedly connected to the surface of the first gear 10 coaxially, a connecting plate 23 is fixedly connected to one side of the upper surface of the movable plate 12, and a threaded hole is formed on the surface of the connecting plate 23 and is screwed with the screw 22 through the threaded hole.
When the screw 22 rotates, the movable plates 12 fixedly connected with the connecting plate 23 can slide on the two guide rails 13, the coating cylinder 16 fixedly connected with the fixed plate 15 repeatedly moves and coats a sample on the surface of the culture substrate 14, meanwhile, the movable plates 12 can make the sample uniformly and non-overlapped distributed on the culture substrate 14 by performing directional movement on the guide rails 13, and the microorganisms originally mixed together are separated on the surface of the culture substrate 14 along the marking part of the coating cylinder 16 by arranging the movable plates 12 and matching among all parts, so that dispersed single bacterial colonies are obtained, pure strains are obtained, and the detection of the microorganisms in the later period is facilitated.
The working principle is as follows: through outside power supply, the outer wall power supply is driving motor, and driving motor output shaft and gear 10 pivot fixed connection drive gear 10 through driving motor and rotate. As shown in fig. 3 and 4, when the first gear 10 rotates, the first gear 10 can rotate the rotary table 24 fixedly connected with the output shaft of the first gear 10, the eccentric column 30 is arranged on the rotary table 24, the movable rod 28 is sleeved on the outer contour of the eccentric column 30, when the eccentric column 30 rotates along with the rotary table 24, the connector 26 on the sliding strip 3 can be pulled through the movable rod 28, the sliding strip 3 is enabled to repeatedly slide in the sliding groove formed in the mounting seat 2 in a limiting manner, and when the sliding strip 3 repeatedly slides in a limiting manner, the connecting column 8 on the rotary table 6 can be enabled to be in contact with the pushing block 25 on the mounting frame 18.
When the connecting column 8 is in contact with the inclined surface of the pushing block 25, the pushing block 25 moves towards one side of the limiting sleeve 20, the connecting column 8 is pushed through the inclined surface, the rotating disc 6 fixedly connected with the rotating column 5 rotates on the mounting column 4, and the outer contour of the rotating column 5 is sleeved with the torsion spring 31; make rolling disc 6 reset under torsional spring 31's effect when spliced pole 8 with promote the separation of piece 25, can make rolling disc 6 rotate repeatedly along with eccentric post 30 lasts to rotate, realize making through the cooperation between spliced pole 8 and each part that storage tank 7 on rolling disc 6 rocks repeatedly, makes the microorganism in the storage tank 7 distribute evenly.
The above-mentioned promotion piece 25 moves to the stop collar 20 side, and the cover is equipped with reset spring 29 through the spline shaft outline position that promotes piece 25 between mounting bracket 18 and promotion piece 25, can make promotion piece 25 reset and promote spliced pole 8, moves and promotes piston push rod 21 and removes in the compression chamber of a suction section of thick bamboo 17 when promoting the spline shaft of piece 25 to stop collar 20 one side, all is provided with the check valve through honeycomb duct 27 liquid outlet and inlet department, under the cooperation of check valve. When the plunger rod 21 is repeatedly moved in the suction cylinder 17, the suction cylinder 17 sucks the microorganism sample in the storage tank 7 into the suction cylinder 17 through the guide pipe 27, injects the microorganism sample into the paint cartridge 16 on the fixing plate 15 through the delivery pipe 19, delivers the microorganism sample onto the culture substrate 14 through the paint cartridge 16, and delivers the paint cartridge 16 onto the culture substrate 14 by providing the paint cartridge 16 and the cooperation between the respective parts.
The sliding bar 3 can repeatedly move in the mounting seat 2 by the continuous rotation of the first gear 10; can make when the removal that sends out repetition in mount pad 2 as slider 3 and slide 3 fixed connection's fixed plate 15 carry out spacing slip in the logical inslot that the outer wall of mount pad 2 was seted up, through two 11 and gear 10 intermeshing, can make two 11 rotations of gear when gear 10 rotates, through two 11 coaxial fixedly connected with screw rods 22 of gear, screw rod 22 carries out the rotation that lasts along with two 11 gears, the screw hole of cooperation screw rod 22 is seted up through connecting plate 23 upper surface.
When the screw 22 rotates, the movable plates 12 fixedly connected with the connecting plate 23 can slide on the two guide rails 13, the coating cylinder 16 fixedly connected with the fixed plate 15 moves repeatedly and coats a sample on the surface of the culture substrate 14, meanwhile, the movable plates 12 move directionally on the guide rails 13, so that the sample can be uniformly distributed on the culture substrate 14 in a non-overlapping manner, the movable plates 12 and the parts are matched, and the microorganisms originally mixed together are separated from the surface of the culture substrate 14 along the scribing part of the coating cylinder 16 to obtain dispersed single colonies, so that pure species can be obtained, and the detection of the microorganisms in the later period is facilitated.
S2, preparation:
ultraviolet disinfection treatment or heating treatment is carried out on the transparent cover 1 of the culture device through external equipment, pollution of external microorganisms to a culture sample is avoided, and meanwhile, a storage tank 7 and a culture substrate 14 which are prepared in advance are respectively placed on a movable plate 12 and a rotating disc 6;
s3, conveying the sample:
the microorganism sample in the storage tank 7 is sucked into the suction cylinder 17 through the guide pipe 27 by the suction cylinder 17 after the piston push rod 21 repeatedly moves in the suction cylinder 17, and is injected into the coating cylinder 16 on the fixed plate 15 through the delivery pipe 19, the microorganism sample is delivered onto the culture substrate 14 through the coating cylinder 16, and the coating cylinder 16 is delivered onto the culture substrate 14 by arranging the coating cylinder 16 and matching the components;
s4, separation and culture:
it can make the slip strip 3 remove in mount pad 2 repeatedly to last the rotation through gear 10, make fixed plate 15 at the spacing slip in the logical inslot of mount pad 2 outer wall, 16 reciprocating motions of coating cylinder on the fixed plate 15 and scribble the sample on culture substrate 14 surfaces, cooperation fly leaf 12 carries out directional movement on guide rail 13 and can make the sample even and not overlap the distribution on culture substrate 14, the microorganism that will mix originally together separates on culture substrate 14 surfaces along 16 marking-off positions of coating cylinder, obtain scattered single bacterial colony, in order to obtain the pure kind, be convenient for detect the microorganism of later stage.
Claims (6)
1. A culture process for detecting microorganisms is characterized by comprising the following operation steps:
s1, designing and manufacturing a culture apparatus:
s2, preparation:
ultraviolet disinfection treatment or heating treatment is carried out on the transparent cover (1) of the culture device through external equipment, so that the pollution of external microorganisms to a culture sample is avoided, and meanwhile, a storage tank (7) and a culture substrate (14) which are prepared in advance are respectively placed on a movable plate (12) and a rotating disc (6);
s3, delivering sample:
the device is characterized in that the device repeatedly moves in an suction cylinder (17) through a piston push rod (21), then the suction cylinder (17) sucks a microorganism sample in a storage tank (7) into the suction cylinder (17) through a guide pipe (27), the microorganism sample is injected into a coating cylinder (16) on a fixed plate (15) through a delivery pipe (19), the microorganism sample is delivered onto a culture substrate (14) through the coating cylinder (16), and the coating cylinder (16) is delivered onto the culture substrate (14) through the arrangement of the coating cylinder (16) and the cooperation of various components;
s4, separation and culture:
the first gear (10) continuously rotates to enable the sliding strip (3) to repeatedly move in the mounting seat (2), the fixing plate (15) slides in a limiting mode in the through groove in the outer wall of the mounting seat (2), the coating barrel (16) on the fixing plate (15) repeatedly moves and coats a sample on the surface of the culture substrate (14), the movable plate (12) is matched to directionally move on the guide rail (13) to enable the sample to be uniformly distributed on the culture substrate (14) in a non-overlapping mode, microorganisms mixed together originally are separated from the surface of the culture substrate (14) along the scribing part of the coating barrel (16), dispersed single colonies are obtained, pure species are obtained, and detection on the microorganisms in the later period is facilitated.
2. The culture process for detecting microorganisms according to claim 1, wherein: the culture device comprises a transparent cover (1) for closed culture, a mounting seat (2) is arranged in the transparent cover (1), two guide rails (13) are fixedly connected to one side of the outer wall of the mounting seat (2), and a movable plate (12) is arranged on each guide rail (13); the movable plate (12) is provided with a through hole and is in limit sliding connection with the guide rail (13) through the through hole, and the upper surface of the movable plate (12) is provided with a culture substrate (14) for culturing microorganisms; an auxiliary device convenient for separating microorganisms is arranged in the mounting seat (2), the auxiliary device comprises a sliding strip (3), and the upper surface of the mounting seat (2) is provided with a sliding groove and is in limit sliding connection with the sliding strip (3) through the sliding groove; the outer wall of the mounting seat (2) is provided with a through groove at one side close to the movable plate (12) and is connected with a fixing plate (15) in a limiting and sliding mode through the through groove, the fixing plate (15) penetrates through the through groove and is fixedly connected with one side of the sliding strip (3), and a coating cylinder (16) for inoculating microorganisms is arranged on the fixing plate (15).
3. The culture process for detecting microorganisms according to claim 2, wherein: the sliding strip (3) is provided with an installation column (4), the surface of the installation column (4) is provided with a through hole and is fixedly connected with a rotating column (5) through the through hole in a fixed-shaft rotating manner, and the upper surface of the rotating column (5) is fixedly connected with a rotating disc (6); a storage tank (7) for culturing microorganisms is fixedly connected to the center of the upper surface of the rotating disc (6), and a torsion spring (31) is sleeved on the outer contour of the rotating column (5); two ends of the torsion spring (31) are respectively and fixedly connected with the mounting column (4) and the rotating disc (6), and a shaking mechanism for shaking the storage tank (7) is arranged on the mounting seat (2);
the pushing mechanism comprises four connecting columns (8) symmetrically arranged on the edge of the upper surface of the only rotating disc (6), and a mounting frame (18) is fixedly connected to one side, close to the fixed plate (15), of the upper surface of the mounting seat (2); a limiting sleeve (20) is arranged on the mounting rack (18), a through groove is formed in the surface of the limiting sleeve (20), and a pushing block (25) matched with the connecting column (8) is connected in a limiting and sliding mode through the through groove; promote piece (25) be located mounting bracket (18) be provided with lead to groove complex integral key shaft, the integral key shaft outline is located mounting bracket (18) and promotes between piece (25) the cover and is equipped with reset spring (29), it sets up for the slope to promote one side that piece (25) is close to spliced pole (8).
4. The culture process for detecting microorganisms according to claim 3, wherein: the upper surface of the mounting seat (2) is provided with a pushing mechanism for pushing the sliding strip (3), the pushing mechanism comprises a connector (26) positioned on one side of the edge of the upper surface of the sliding strip (3), an inserting tip is fixedly connected between the inner walls of two sides of the connector (26), and a movable rod (28) is sleeved on the outer contour of the inserting tip; the one end that connector (26) were kept away from in movable rod (28) has seted up the through-hole and has rotated through this through-hole and be connected with eccentric post (30), mount pad (2) one side fixedly connected with mounting panel (9), dead axle rotation connected carousel (24) have been seted up to mounting panel (9) upper surface, carousel (24) and eccentric post (30) fixed connection.
5. The culture process for detecting microorganisms according to claim 4, wherein: a flow guide pipe (27) is arranged in the storage tank (7), one end of the flow guide pipe (27), which is far away from the connecting column (8), is communicated with a liquid inlet of the suction barrel (17), and a check valve is arranged between the flow guide pipe (27) and the suction barrel (17) in a communicated manner; the liquid outlet of the flow guide pipe (27) is connected with a conveying pipe (19) in a penetrating way; conveyer pipe (19) link up the department with mounting bracket (18) and are provided with the reverse valve, the fixed and coating barrel (16) through connection of one end of mounting bracket (18) are kept away from in conveyer pipe (19), honeycomb duct (27) and conveyer pipe (19) are the hose.
6. The culture process for detecting microorganisms according to claim 5, wherein: a through hole is formed in one side, close to the first gear (10), of the surface of the mounting plate (9), a second gear (11) is connected with the through hole in a fixed-shaft rotating mode, and the second gear (11) is meshed with the first gear (10); the surface of the first gear (10) is coaxially and fixedly connected with a screw rod (22), one side of the upper surface of the movable plate (12) is fixedly connected with a connecting plate (23), and a threaded hole is formed in the surface of the connecting plate (23) and is in threaded connection with the screw rod (22) through the threaded hole.
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| CN202210646577.4A CN115109706A (en) | 2022-06-09 | 2022-06-09 | Culture process for microbial detection |
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| US4696902A (en) * | 1984-06-06 | 1987-09-29 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Modular apparatus for cell culture |
| KR20200046284A (en) * | 2018-10-24 | 2020-05-07 | 대한민국(농촌진흥청장) | Real time automatic device for detecting hazardous microbe |
| WO2020151368A1 (en) * | 2019-01-23 | 2020-07-30 | 安图实验仪器(郑州)有限公司 | Fully automatic microorganism identification and drug sensitivity analysis system |
| CN113322168A (en) * | 2021-05-28 | 2021-08-31 | 冯成金 | Food microorganism detection device and detection method thereof |
| US20220033754A1 (en) * | 2020-07-31 | 2022-02-03 | Liu Binlu | Microorganism inoculation device |
| CN216337611U (en) * | 2021-01-08 | 2022-04-19 | 肖怿 | Microbial cultivation device |
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2022
- 2022-06-09 CN CN202210646577.4A patent/CN115109706A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4696902A (en) * | 1984-06-06 | 1987-09-29 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Modular apparatus for cell culture |
| KR20200046284A (en) * | 2018-10-24 | 2020-05-07 | 대한민국(농촌진흥청장) | Real time automatic device for detecting hazardous microbe |
| WO2020151368A1 (en) * | 2019-01-23 | 2020-07-30 | 安图实验仪器(郑州)有限公司 | Fully automatic microorganism identification and drug sensitivity analysis system |
| US20220033754A1 (en) * | 2020-07-31 | 2022-02-03 | Liu Binlu | Microorganism inoculation device |
| CN216337611U (en) * | 2021-01-08 | 2022-04-19 | 肖怿 | Microbial cultivation device |
| CN113322168A (en) * | 2021-05-28 | 2021-08-31 | 冯成金 | Food microorganism detection device and detection method thereof |
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