CN220674717U

CN220674717U - Aseptic environment manufacturing device for grape tissue culture

Info

Publication number: CN220674717U
Application number: CN202321866747.6U
Authority: CN
Inventors: 张超
Original assignee: Individual
Current assignee: Individual
Priority date: 2023-07-17
Filing date: 2023-07-17
Publication date: 2024-03-29
Anticipated expiration: 2033-07-17

Abstract

The utility model provides a device for manufacturing a sterile environment for grape tissue culture, and belongs to the technical field of culture. Solves the problems that the steps of the prior art for manufacturing the sterile environment are complicated, the gas is not disinfected, and the influence of disinfectant molecules on tissue culture cannot be eliminated. The device comprises a pressing exhaust shell mechanism, a linkage liquid discharge limiting mechanism, an injection transfer mechanism, a molecule removing mechanism and a press, wherein the press is communicated with an upper end opening of the pressing exhaust shell mechanism, one side, far away from the press, of the pressing exhaust shell mechanism is connected with the linkage liquid discharge limiting mechanism, the injection transfer mechanism is connected with the molecule removing mechanism, the injection transfer mechanism is used for pressurizing and injecting an inhaled disinfectant into the molecule removing mechanism and injecting an injected molecule removing mechanism to be connected with a mixed gas source, and the injected molecule removing mechanism is used for disinfecting gas and removing disinfectant molecules. It is mainly used for the aseptic environment manufacture of tissue culture.

Description

Aseptic environment manufacturing device for grape tissue culture

Technical Field

The utility model relates to the technical field of cultivation, in particular to a device for manufacturing a sterile environment for grape tissue culture.

Background

Plant tissue culture can be divided into four stages: firstly, a sterile system is established, namely, the explant and the culture medium are sterilized and inoculated, and the callus or organ is obtained. And secondly, proliferation is carried out, new plants are continuously differentiated, or adventitious buds and embryoids are directly produced, and subculture can be repeatedly carried out according to the needs, so that the aim of mass propagation is fulfilled. Thirdly, transferring the plants to rooting culture, and transferring the plants to rooting culture medium or directly cutting the plants to perform cutting rooting. Fourthly, the transition of the test tube seedling, namely, the adaptation process of the test tube seedling to the external environment is carried out for a certain time after the test tube seedling comes out of the bottle.

Tissue culture of grapes also requires a sterile environment. The sterile environment system of the first step is important for tissue culture, and no stable and reliable sterile environment exists, so that subsequent work is not conducted. When the traditional sterile environment is maintained, in order to ensure tightness, the compressor needs to continuously work in the process to ensure the tightness of the environment, and the operation cost is high.

The tightness of the container also affects the stability of the sterile environment, and in the existing treatment means, multi-step operation is required to achieve sealing and manufacturing of the sterile environment, so that the efficiency is low, the pressure maintaining effect is not ideal, and the pressure maintaining effect is not ideal, so that some bacteria enter the culture environment again, and the sterile environment is invalid.

Disclosure of Invention

The utility model aims to provide a device for manufacturing a sterile environment for grape tissue culture, which solves the problems that the steps for manufacturing the sterile environment by the prior art are complicated, the gas is not disinfected, and the influence of disinfectant molecules on tissue culture cannot be eliminated.

In order to achieve the above purpose, the utility model provides a device for manufacturing a sterile environment for grape tissue culture, which comprises the following specific technical scheme:

the utility model provides a grape tissue culture is with aseptic environment manufacturing installation, includes presses exhaust housing mechanism and press machine, press the upper end opening intercommunication of exhaust housing mechanism with pressing, be equipped with linkage flowing back stop gear on the exhaust housing mechanism of pressing, draw and penetrate transfer mechanism and molecule and get rid of the mechanism, press exhaust housing mechanism to keep away from press machine one side and link to each other with linkage flowing back stop gear, linkage flowing back stop gear slides and sets up in drawing and draws the transfer mechanism, draw and remove the mechanism to link to each other with the molecule, press exhaust housing mechanism and be used for receiving the pressure after self partial gas and to spray the gaseous secondary filtration that the molecule got rid of the mechanism carried, linkage flowing back stop gear is used for pressing the one-way spacing and to draw the transfer mechanism pressurization of drawing of the pressurized movement of pressing exhaust housing mechanism, draw the transfer mechanism and be used for spraying into the molecule removal mechanism and draw the external mixed air supply of molecule removal mechanism with the pressurized, the molecule removal mechanism is used for sterilizing gas and getting rid of disinfectant molecule;

the utility model provides a press exhaust shell mechanism, including last casing, oxygen sensor, compression pipe, base, step motor, shell, opening, exhaust hole, holding opening, rotatory filter core and intercommunication mouth of pipe, the position sets up the intercommunication mouth of pipe by last position on the lateral wall of one side of going up the casing, oxygen sensor one end sets up and is close to intercommunication mouth of pipe one side on the inside roof of last casing, go up casing, compression pipe and base and connect gradually from top to bottom, the shell is connected on last casing outer wall and is located the orificial offside of intercommunication, rotatory filter core rotates to be connected in the shell, step motor's rotation end rotates with the shell to be connected and links to each other with rotatory filter core, the opening runs through shell and last casing in proper order and with rotatory filter core center eccentric setting, the exhaust hole sets up and keeps away from step motor one side on last casing lower terminal surface, last casing compresses compression pipe to limit time compression pipe cover exhaust hole, the holding opening sets up on the terminal surface under the base and communicates with compression pipe and last casing in proper order.

Further, press the exhaust shell mechanism and still include spout, triangle cross-section spacing groove, overhaul and dismantle buckle, annular cushion and plate body, the base is kept away from intercommunication mouth of pipe one side and is set up the plate body, the plate body sets up a plurality of triangle cross-section spacing grooves of evenly arranging along its length direction in the middle part, the spout is provided with two and is bilateral symmetry distribution on the plate body in contrast in triangle cross-section spacing groove, overhaul and dismantle the buckle setting on the shell lateral wall, annular cushion sets up on the base lower terminal surface and with the concentric setting of holding opening.

Further, the diameter of the accommodating opening is smaller than or equal to the diameter of the inner ring of the annular cushion.

Further, the pressing exhaust shell mechanism further comprises a rubber ring, wherein the rubber ring is fixedly arranged at the edge of the lower end face of the upper shell, and the diameter of the rubber ring is the same as the diameter of the outer circle of the compression pipe.

Further, the pressing exhaust shell mechanism further comprises a shaft-bearing impeller and a driving motor, the shaft-bearing impeller is rotationally connected to the side wall of the upper shell and is located under the communicating pipe opening, the shaft-bearing impeller is located in the upper shell and forms air flow in the direction of the rotary filter element when rotating, the rotating end of the driving motor is connected with the shaft-bearing impeller, and the driving motor is fixedly connected to the outer wall of the upper shell.

Further, the linkage flowing back stop gear includes connecting rod, movable supporting seat, spacing spout, presses seat, area pole piston, spliced pole, spring and triangle cross-section stopper, connecting rod one end articulates on last casing lateral wall and is located rotatory filter core under, the other end of connecting rod articulates on the movable supporting seat, set up spacing spout on the movable supporting seat, press the seat slip setting in spacing spout, the lower terminal surface of pressing the seat sets up the spliced pole, the spliced pole slidable passes the movable supporting seat and connects on the up end of triangle cross-section stopper, the spring housing is on the spliced pole and both ends are connected respectively on the up end of the lower terminal surface of movable supporting seat and triangle cross-section stopper, the movable supporting seat lower extreme sets up two T shape sliders, and the slip of two T shape sliders one-to-one sets up in the spout, triangle cross-section stopper cooperatees with triangle cross-section spacing groove, area pole piston connection is kept away from on the casing one side on the movable supporting seat lateral wall.

Further, draw and transfer mechanism including pressure change section of thick bamboo, hydrojet pipe, feed liquor check valve and play liquid check valve, the gliding pressure change section of thick bamboo that passes of pole piston of taking is connected on the piston of taking the pole piston, the piston slip setting of taking the pole piston is in the pressure change section of thick bamboo, hydrojet pipe and feed liquor pipe intercommunication are on the pressure change section of thick bamboo, set up the feed liquor check valve in the feed liquor pipe, play liquid check valve sets up in the hydrojet pipe.

Further, the injection transfer mechanism further comprises a cylinder support, and the pressure change cylinder is connected to the plate body through the cylinder support.

Further, the molecular removing mechanism comprises a telescopic hose, a coiled pipe rack, an injection pipe, an inlet pipe and an air inlet one-way valve, wherein one end of the telescopic hose is communicated with the opening, the other end of the telescopic hose is communicated with the outlet end of the coiled pipe, the inlet end of the coiled pipe is communicated with the outlet end of the injection pipe, the inlet end of the injection pipe is provided with the inlet pipe and the air inlet one-way valve is arranged in the inlet pipe, and the coiled pipe is connected with the plate body through the coiled pipe rack.

Further, the injection pipe including the first horizontal side pipe, slope side pipe and the second horizontal side pipe of intercommunication in proper order, first horizontal side pipe and coiled pipe intercommunication, second horizontal side pipe and import pipe intercommunication, first horizontal side pipe highly is higher than second horizontal side pipe, spray pipe links to each other with second horizontal side pipe and spray pipe extends to in the second horizontal side pipe and the mouth of pipe is towards slope side pipe one side.

The utility model has the advantages that:

according to the utility model, the upper shell is pressed in a one-step manner, the compression pipe is pressed down by the upper shell to discharge air in the compression pipe, in the process of pressing and exhausting, the linkage liquid discharge limiting mechanism is synchronously driven to move by the pressing action, the linkage liquid discharge limiting mechanism realizes one-way limiting in the moving process, so that the aseptic environment is prevented from being invalid, in addition, the injection transfer mechanism can be pressurized in the moving process of the linkage liquid discharge limiting mechanism, so that the disinfectant in the injection transfer mechanism is injected into the molecular removal mechanism in a pressurized manner, the external air source of the molecular removal mechanism is injected, the aseptic environment for one-step pressing and exhausting and preventing pressure release is achieved, the introduced mixed gas can be sterilized by utilizing the pressure relation of the pressing process, the influence of disinfectant molecules on the tissue culture process is reduced, the rotary filtering of the rotary filter element is utilized to play a role of secondary filtering, no disinfectant molecules in the aseptic environment is fully ensured, and the influence of disinfectant molecules on tissue culture is avoided.

Drawings

The utility model will be described in further detail with reference to the accompanying drawings and detailed description.

FIG. 1 is a schematic perspective view of a first view of a device for manufacturing a sterile environment for tissue culture of grapes according to the present utility model;

FIG. 2 is a schematic view showing a second perspective view of a device for manufacturing a sterile environment for tissue culture of grapes according to the present utility model;

FIG. 3 is a schematic diagram showing the overall sectional structure of a device for manufacturing a sterile environment for tissue culture of grapes according to the present utility model;

FIG. 4 is a schematic view of a first perspective view of the press vent housing mechanism of the present utility model;

FIG. 5 is a schematic view of a second perspective view of the press vent housing mechanism of the present utility model;

FIG. 6 is a schematic view of a third perspective view of the press vent housing mechanism of the present utility model;

FIG. 7 is a schematic cross-sectional view of the mechanism for depressing the vent housing of the present utility model;

FIG. 8 is a schematic view of a first perspective view of the linked drain stop mechanism of the present utility model;

FIG. 9 is a schematic view of a second perspective view of the linked drain stop mechanism of the present utility model;

FIG. 10 is a schematic perspective view of an injection transfer mechanism of the present utility model;

FIG. 11 is a schematic cross-sectional view of the injection transfer mechanism of the present utility model;

FIG. 12 is a schematic perspective view of a molecular removal mechanism according to the present utility model;

FIG. 13 is a schematic cross-sectional structural view of a molecular removal mechanism of the present utility model.

In the figure: pressing the exhaust housing mechanism 1; an upper case 1-1; an oxygen sensor 1-2; compression tube 1-3; 1-4 parts of a base; 1-5 of sliding grooves; 1-6 parts of triangular section limiting grooves; 1-7 of stepping motors; 1-8 of a shell; overhauling and disassembling the pinch plates 1-9; 1-10 openings; 1-11 parts of exhaust holes; the accommodating openings 1-12; annular cushions 1-13; rotating the filter element 1-14; 1-15 parts of impeller with shaft; driving motors 1-16; 1-17 of communicating pipe orifices; 1-18 parts of rubber rings; 1-19 parts of plate body; a linkage liquid discharge limiting mechanism 2; a connecting rod 2-1; 2-2 of movable supporting seats; 2-3 of a limiting chute; pressing the base 2-4; 2-5 of a piston with a rod; 2-6 of connecting columns; 2-7 of springs; 2-8 of triangular section limiting blocks; an injection transfer mechanism 3; the cylinder supports 3-1; a pressure change cylinder 3-2; a liquid spraying pipe 3-3; 3-4 parts of liquid inlet pipe; 3-5 parts of liquid inlet one-way valve; 3-6 parts of liquid outlet one-way valve; a molecule removal mechanism 4; a flexible hose 4-1; a serpentine tube 4-2; 4-3 of a serpentine pipe rack; 4-4 parts of an injection pipe; inlet pipe 4-5; 4-6 parts of an air inlet one-way valve; and a press 5.

Description of the embodiments

The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that the terms center, upper, lower, left, right, vertical, horizontal, inner, outer, etc. indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element to be referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms first, second, and third are used for descriptive purposes only and are not to be construed as indicating or implying any relative importance.

As shown in fig. 1-7, a device for manufacturing a sterile environment for grape tissue culture comprises a pressing exhaust shell mechanism 1 and a press 5, wherein the press 5 is communicated with an upper end opening of the pressing exhaust shell mechanism 1, a linkage liquid discharge limiting mechanism 2, an injection transfer mechanism 3 and a molecule removing mechanism 4 are arranged on the pressing exhaust shell mechanism 1, one side of the pressing exhaust shell mechanism 1 far away from the press 5 is connected with the linkage liquid discharge limiting mechanism 2, the linkage liquid discharge limiting mechanism 2 is arranged in the injection transfer mechanism 3 in a sliding manner, the injection transfer mechanism 3 is connected with the molecule removing mechanism 4, the pressing exhaust shell mechanism 1 is used for discharging partial gas after being subjected to pressure and secondarily filtering the gas conveyed by the injection molecule removing mechanism 4, the linkage liquid discharge limiting mechanism 2 is used for unidirectionally limiting the pressed movement of the pressing exhaust shell mechanism 1 and pressurizing the injection transfer mechanism 3, the injection transfer mechanism 3 is used for pressurizing the sucked disinfection liquid into the molecule removing mechanism 4 and externally connecting a mixed gas source, and the molecule removing mechanism 4 is used for disinfecting gas and removing molecules;

through pressing the exhaust shell mechanism 1 of pressing for the outside exhaust of air in the exhaust shell mechanism 1 of pressing, the exhaust in-process can form and get rid of the mechanism 4 to the gas flow of pressing the exhaust shell mechanism 1 by the molecule, can drive the synchronous motion of linkage flowing back stop gear 2 in the in-process of pressing the motion of exhaust shell mechanism 1, linkage flowing back stop gear 2 can cooperate and press the exhaust shell mechanism 1 to carry out one-way spacing, prevent to press the deformation that exhaust shell mechanism 1 resumes the in-process of pressing and take place, the motion through linkage flowing back stop gear 2 pressurizes the injection transfer mechanism 3, make the injection transfer mechanism 3 in the initial absorptive disinfectant enter into the molecule under the effect of pressure and get rid of mechanism 4, and utilize the pressure that forms in the injection process to draw the molecule to get rid of mechanism 4 external air supply's of mechanism of injection to carry out intensive mixing with the disinfectant and disinfect, and disinfectant molecule is eliminated in the in-process of drawing the molecule removal mechanism 4 motion, finally cooperate the filtration effect of pressing the exhaust shell mechanism 1 to remove the disinfectant to tissue culture.

The pressing exhaust shell mechanism 1 comprises an upper shell 1-1, an oxygen sensor 1-2, a compression pipe 1-3, a base 1-4, a stepping motor 1-7, a shell 1-8, an opening 1-10, an exhaust hole 1-11, a containing opening 1-12, a rotary filter element 1-14 and a communication pipe orifice 1-17, wherein the communication pipe orifice 1-17 is arranged on one side wall of the upper shell 1-1 near the upper side of the communication pipe orifice 1-17 on the inner top wall of the upper shell 1-1, one end of the oxygen sensor 1-2 is arranged on one side, close to the communication pipe orifice 1-17, of the upper shell 1-1, the compression pipe 1-3 and the base 1-4 are sequentially connected from top to bottom, the shell 1-8 is connected on the outer wall of the upper shell 1-1 and is positioned on the opposite side of the communication pipe orifice 1-17, the rotary filter element 1-14 is rotatably connected in the shell 1-8, the rotary end of the stepping motor 1-7 is rotatably connected with the shell 1-8 and is connected with the rotary filter element 1-14, the opening 1-10 sequentially penetrates through the shell 1-8 and the upper shell 1-3 and is arranged on one side, close to the communication pipe orifice 1-17, and is far from the upper end face of the upper shell 1-3 and the compression pipe 1-3 and the base 1-3 is arranged on the eccentric end face of the upper shell 1-1.

The compression tube 1-3 is extruded by pressing the upper shell 1-1, so that gas in the compression tube 1-3 is discharged from the exhaust hole 1-11, an internal low-pressure environment is manufactured in an initial state, the internal gas is discharged to facilitate subsequent replacement of the gas, sterile and sterilized gas is introduced into the upper shell 1-1, the filter element 1-14 is rotated under the driving of the stepping motor 1-7 to intermittently rotate, the mixed gas conveyed by the injection molecule removing mechanism 4 is filtered, the influence of a disinfectant on cell culture is prevented, the oxygen concentration in the upper shell 1-1 can be detected by the oxygen sensor 1-2, and feedback of test data is facilitated.

As shown in fig. 4-7, the pressing exhaust shell mechanism 1 further comprises a sliding groove 1-5, triangular section limiting grooves 1-6, overhauling and disassembling buckle plates 1-9, an annular cushion 1-13 and a plate body 1-19, wherein the plate body 1-19 is arranged on one side, far away from the communicating pipe orifice 1-17, of the base 1-4, a plurality of triangular section limiting grooves 1-6 which are uniformly arranged are arranged in the middle of the plate body 1-19 along the length direction of the plate body, the sliding groove 1-5 is provided with two and symmetrically distributed on the plate body 1-19 in a two-sided mode compared with the triangular section limiting grooves 1-6, the overhauling and disassembling buckle plates 1-9 are arranged on the side wall of the shell 1-8, and the annular cushion 1-13 is arranged on the lower end face of the base 1-4 and is concentrically arranged with the accommodating opening 1-12.

The diameter of the accommodating opening 1-12 is smaller than or equal to the inner ring diameter of the annular cushion 1-13.

Through the cooperation of triangle cross-section spacing groove 1-6 and linkage flowing back stop gear 2, can form the effect of one-way spacing to prevent compressed compression pipe 1-3 and resume deformation, be favorable to realizing aseptic airtight environment's maintenance, the setting of annular cushion 1-13 can guarantee that base 1-4 keeps good sealedly rather than the plane that contacts, thereby prevents that accommodation opening 1-12 part from taking place the phenomenon with external intercommunication, thereby guarantee aseptic environment's continuation reliability.

As shown in fig. 4-7, the pressing exhaust casing mechanism 1 further comprises a rubber ring 1-18, wherein the rubber ring 1-18 is fixedly arranged at the edge of the lower end face of the upper casing 1-1, and the diameter of the rubber ring is the same as the diameter of the outer circle of the compression pipe 1-3.

Through the arrangement of the rubber ring 1-18, the upper casing 1-1 and the compression pipe 1-3 can be effectively attached to the compression pipe 1-3 through the rubber ring 1-18 after the compression pipe 1-3 is compressed, so that the exhaust hole 1-11 is blocked to ensure the maintenance of a sterile closed environment, and the exhaust effect of the press 5 on the pressing exhaust shell mechanism 1 is facilitated.

As shown in fig. 4-7, the pressing exhaust casing mechanism 1 further includes a shaft-bearing impeller 1-15 and a driving motor 1-16, the shaft-bearing impeller 1-15 is rotatably connected to the side wall of the upper casing 1-1 and is located right below the communication pipe orifice 1-17, the impeller of the shaft-bearing impeller 1-15 is located in the upper casing 1-1 and forms an air flow in the direction of rotating the filter element 1-14 when rotating, the rotating end of the driving motor 1-16 is connected with the shaft-bearing impeller 1-15, and the driving motor 1-16 is fixedly connected to the outer wall of the upper casing 1-1.

When the press 5 runs to carry out secondary negative pressure treatment, the press 5 forms negative pressure at the communicating pipe orifice 1-17, the rotation of the impeller 1-15 with the shaft can form pushing air flow, the pushing air flow is gradually lifted under the suction of the negative pressure of the communicating pipe orifice 1-17 to be directly mixed with the air entering from the opening 1-10 and then moves towards the communicating pipe orifice 1-17, so that the air moving to the position of the oxygen sensor 1-2 basically meets uniformity, the oxygen content measured at the position basically represents the concentration of oxygen in the whole air in the upper shell 1-1, the oxygen concentration can be judged in real time, and the control of the index of the tissue culture environment is more facilitated.

The linkage liquid draining and limiting mechanism 2 comprises a connecting rod 2-1, a movable supporting seat 2-2, a limiting chute 2-3, a pressing seat 2-4, a piston 2-5 with a rod, a connecting column 2-6, a spring 2-7 and a triangular section limiting block 2-8, wherein one end of the connecting rod 2-1 is hinged on the side wall of the upper shell 1-1 and is positioned right below the rotary filter element 1-14, the other end of the connecting rod 2-1 is hinged on the movable supporting seat 2-2, the movable supporting seat 2-2 is provided with the limiting chute 2-3, the pressing seat 2-4 is slidably arranged in the limiting chute 2-3, the lower end face of the pressing seat 2-4 is provided with the connecting column 2-6, the connecting column 2-6 slidably penetrates through the movable supporting seat 2-2 and is connected to the upper end face of the triangular section limiting block 2-8, the spring 2-7 is sleeved on the connecting column 2-6, two ends of the spring are respectively connected to the lower end face of the movable supporting seat 2-8 and the upper end face of the triangular section limiting block 2-8, the two T-shaped sliding blocks are correspondingly arranged on the two sides of the upper end faces of the triangular section limiting block 2-8, and the two T-shaped sliding blocks are correspondingly arranged on the two sides of the side faces of the triangular supporting seat 2-1-2 and are correspondingly arranged on the side of the triangular section limiting block 2-1.

Through the cooperation of triangle cross section stopper 2-8 and triangle cross section spacing groove 1-6, can realize the effect of one-way spacing, in last casing 1-1 downward movement passes through connecting rod 2-1 and drives movable support seat 2-2 motion, under the elastic action of spring 2-7, triangle cross section stopper 2-8 can be continuous from last triangle cross section spacing groove 1-6 roll-off get into in the next triangle cross section spacing groove 1-6, this process can prevent movable support seat 2-2 reverse motion to play the one-way spacing effect to last casing 1-1.

As shown in fig. 10 and 11, the injection transfer mechanism 3 includes a pressure change cylinder 3-2, a liquid spraying pipe 3-3, a liquid inlet pipe 3-4, a liquid inlet one-way valve 3-5 and a liquid outlet one-way valve 3-6, a rod of the rod piston 2-5 passes through the pressure change cylinder 3-2 in a sliding manner and is connected to a piston of the rod piston 2-5, the piston of the rod piston 2-5 is arranged in the pressure change cylinder 3-2 in a sliding manner, the liquid spraying pipe 3-3 and the liquid inlet pipe 3-4 are communicated on the pressure change cylinder 3-2, the liquid inlet one-way valve 3-5 is arranged in the liquid inlet pipe 3-4, and the liquid outlet one-way valve 3-6 is arranged in the liquid spraying pipe 3-3.

In the process of unidirectional movement of the movable supporting seat 2-2, the movable supporting seat 2-2 drives the rod-carrying piston 2-5 to move, and the rod-carrying piston 2-5 moves in the pressure change cylinder 3-2, so that disinfectant stored in the pressure change cylinder 3-2 in advance is pressurized, and the pressurized disinfectant is enabled to rush out of the liquid outlet unidirectional valve 3-6 and then is sprayed out of the liquid spraying pipe 3-3.

As shown in fig. 1, 4 and 10, the injection transfer mechanism 3 further comprises a cylinder support 3-1, and the pressure change cylinder 3-2 is connected to the plate body 1-19 through the cylinder support 3-1.

The pressure change cylinder 3-2 can be supported by the cylinder support 3-1, and the injection transfer mechanism 3 is also convenient to install on the plate body 1-19.

The molecular removing mechanism 4 comprises a telescopic hose 4-1, a coiled pipe 4-2, a coiled pipe rack 4-3, an ejector pipe 4-4, an inlet pipe 4-5 and an air inlet one-way valve 4-6, wherein one end of the telescopic hose 4-1 is communicated with an opening 1-10, the other end of the telescopic hose is communicated with the outlet end of the coiled pipe 4-2, the inlet end of the coiled pipe 4-2 is communicated with the outlet end of the ejector pipe 4-4, the inlet end of the ejector pipe 4-4 is provided with the inlet pipe 4-5, the air inlet one-way valve 4-6 is arranged in the inlet pipe 4-5, and the coiled pipe 4-2 is connected with the plate body 1-19 through the coiled pipe rack 4-3.

The ejector tube 4-4 comprises a first horizontal square tube, an inclined square tube and a second horizontal square tube which are sequentially communicated, wherein the first horizontal square tube is communicated with the coiled tube 4-2, the second horizontal square tube is communicated with the inlet tube 4-5, the height of the first horizontal square tube is higher than that of the second horizontal square tube, the liquid spraying tube 3-3 is connected with the second horizontal square tube, and the liquid spraying tube 3-3 extends into the second horizontal square tube and the mouth of the liquid spraying tube faces one side of the inclined square tube.

The disinfectant sprayed out of the liquid spraying pipe 3-3 is matched with the gas flow produced in the process of exhausting the gas from the exhaust hole 1-11 in the pressing process, and the superposition of the pressure of the gas and the gas can form an injection effect on the air inlet one-way valve 4-6, so that the air inlet one-way valve 4-6 is opened, the mixed gas externally connected with the inlet pipe 4-5 can enter the injection pipe 4-4 from the inlet pipe 4-5, the gas can be fully contacted with the disinfectant at the position due to the injection effect, and the gas can collide with the inclined square pipe inner arm of the injection pipe 4-4 in the process of spraying the disinfectant to play a role of upwards sputtering, so that the distribution range of the disinfectant is further enlarged, the gas and the disinfectant are fully contacted, the disinfection is ensured to be completed instantaneously, disinfectant molecules are gradually hung on the wall and are fixed by the coiled pipe 4-2, the content of the disinfectant in the gas is reduced, and the influence of the disinfectant on tissue culture is prevented.

Working principle:

before the device is used, a pipeline capable of continuously supplementing disinfectant is externally connected with the liquid inlet pipe 3-4, a mixed gas pipeline for manufacturing a sterile environment is externally connected with the inlet pipe 4-5, the tissue culture dish is placed on the operation platform, then the device is placed on the operation platform from top to bottom, the tissue culture dish enters the upper shell 1-1 from the containing opening 1-12, the base 1-4 is attached to the platform on which the culture dish is placed, and the annular cushion 1-13 is tightly attached to the platform, so that the sealing effect is realized.

The upper housing 1-1 is pressed downward, the compression tube 1-3 is compressed by the downward movement of the upper housing 1-1, and gas is continuously discharged from the exhaust hole 1-11 in the compression process, so that the gas flow from the inlet tube 4-5 to the exhaust hole 1-11 is formed in the device, and a part of oxygen is discharged in the compression process. In the process, the upper shell 1-1 moves downwards to drive the movable supporting seat 2-2 to move through the connecting rod 2-1, the movable supporting seat 2-2 applies pressure to the pressure change cylinder 3-2 through the rod-carried piston 2-5, so that disinfectant pre-stored in the pressure change cylinder 3-2 is pressurized and then rushes out of the liquid outlet one-way valve 3-6 to be discharged from the liquid spraying pipe 3-3, pressurized liquid sprayed by the liquid spraying pipe 3-3 forms a back pressure area at one side of the injection pipe 4-4 close to the inlet pipe 4-5, and thus under the dual action of the outward flowing gas pressure formed by compression of the compression pipe 1-3 and the back pressure formed by the injection pipe 4-4, the air inlet one-way valve 4-6 is opened, and mixed gas enters the injection pipe 4-4 through the inlet pipe 4-5, and because of the pressure effect of the pressurized liquid sprayed by the spray pipe 3-3, the gas can be preferentially converged to the liquid, so that the effect of fully contacting the gas and the disinfectant is achieved, and the instant disinfection is achieved, and because of the effect of the inclined square pipe in the three-section structure of the injection pipe 4-4, the disinfectant impacts the inner wall of the inclined square pipe to perform the effect of turning upwards to sputter, the coverage area of the liquid is enlarged, the disinfection effect is enhanced again, the disinfected gas enters the coiled pipe 4-2 to be turned repeatedly, so that disinfectant molecules gradually settle down, finally, the disinfectant molecules are filtered again by the rotary filter element 1-14 through the telescopic hose 4-1 and the opening 1-10, the removal of the disinfectant molecules is fully ensured, and then the gas enters the upper shell 1-1, so that the effect of one-time pressing real-time ventilation is achieved, so that the gas is changed from air into sterilized sterile air.

When the process can not reach the required sterile environment, the press 5 is started, negative pressure is formed at the position of the communicating pipe orifice 1-17 by the press 5, so that gas externally connected with a gas source of the inlet pipe 4-5 is continuously led into the device, the content of bacteria-containing gas is continuously reduced, the gas which enters subsequently can be directly disinfected by the disinfectant on the inner wall of the coiled pipe 4-2 when passing through the coiled pipe 4-2, the disinfection effect is ensured and the waste phenomenon is prevented on the premise of controlling the dosage of the single disinfectant on the whole, and the reliable sterile environment is achieved.

After the cultivation is finished, the pressing seat 2-4 is lifted upwards, and then the upper shell 1-1 is pulled to reset, so that the piston 2-5 with the rod is reset, the liquid inlet one-way valve 3-5 is opened, and disinfectant is supplemented.

Of course, the above description is not intended to limit the utility model, but rather the utility model is not limited to the above examples, and variations, modifications, additions or substitutions within the spirit and scope of the utility model will be within the scope of the utility model.

Claims

1. The aseptic environment manufacturing device for grape tissue culture comprises a pressing exhaust shell mechanism (1) and a press (5), wherein the press (5) is communicated with an upper end opening of the pressing exhaust shell mechanism (1), and the aseptic environment manufacturing device is characterized in that a linkage liquid discharge limiting mechanism (2), an injection transfer mechanism (3) and a molecule removing mechanism (4) are arranged on the pressing exhaust shell mechanism (1), one side, far away from the press (5), of the pressing exhaust shell mechanism (1) is connected with the linkage liquid discharge limiting mechanism (2), the linkage liquid discharge limiting mechanism (2) is slidably arranged in the injection transfer mechanism (3), the injection transfer mechanism (3) is connected with a molecule removing mechanism (4), the pressing exhaust shell mechanism (1) is used for discharging partial gas after being subjected to pressure and secondarily filtering gas conveyed by the injection molecule removing mechanism (4), the linkage liquid discharge limiting mechanism (2) is used for one-way limiting the pressed movement of the pressing exhaust shell mechanism (1) and pressurizing the injection transfer mechanism (3), and the injection transfer mechanism (3) is used for pressurizing an inhaled sterilizing liquid to remove molecules (4) and injecting the injected sterilizing liquid into the molecule removing mechanism (4) to remove mixed gas, and the sterilizing gas is externally connected with the sterilizing gas source (4);

the pressing exhaust shell mechanism (1) comprises an upper shell (1-1), an oxygen sensor (1-2), a compression pipe (1-3), a base (1-4), a stepping motor (1-7), a shell (1-8), an opening (1-10), an exhaust hole (1-11), a containing opening (1-12), a rotary filter element (1-14) and a communicating pipe orifice (1-17), wherein the communicating pipe orifice (1-17) is arranged on the upper side wall of one side of the upper shell (1-1), one end of the oxygen sensor (1-2) is arranged on the inner top wall of the upper shell (1-1) and is close to one side of the communicating pipe orifice (1-17), the upper shell (1-1), the compression pipe (1-3) and the base (1-4) are sequentially connected from top to bottom, the shell (1-8) is connected to the outer wall of the upper shell (1-1) and is positioned on the opposite side of the communicating pipe orifice (1-17), the rotary filter element (1-14) is rotationally connected in the shell (1-8), the rotating end of the stepping motor (1-7) is rotationally connected with the rotating shell (1-8) and rotationally connected with the filter element (1-8), the opening (1-10) sequentially penetrates through the shell (1-8) and the upper shell (1-1) and is eccentrically arranged at the center of the rotary filter element (1-14), the exhaust hole (1-11) is formed in one side, far away from the stepping motor (1-7), of the lower end face of the upper shell (1-1), the upper shell (1-1) compresses the compression pipe (1-3) to the limit, the compression pipe (1-3) covers the exhaust hole (1-11), and the accommodating opening (1-12) is formed in the lower end face of the base (1-4) and is sequentially communicated with the compression pipe (1-3) and the upper shell (1-1).

2. The apparatus for producing a sterile environment for tissue culture of grape according to claim 1, wherein: the pressing exhaust shell mechanism (1) further comprises a sliding groove (1-5), triangular section limiting grooves (1-6), overhauling and disassembling buckle plates (1-9), annular cushions (1-13) and a plate body (1-19), wherein the plate body (1-19) is arranged on one side of the base (1-4) away from the communicating pipe orifice (1-17), a plurality of triangular section limiting grooves (1-6) which are uniformly distributed are arranged in the middle of the plate body (1-19) along the length direction of the plate body, the sliding groove (1-5) is provided with two triangular section limiting grooves and symmetrically distributed on the plate body (1-19) on two sides compared with the triangular section limiting grooves (1-6), the overhauling and disassembling buckle plates (1-9) are arranged on the side wall of the shell (1-8), and the annular cushions (1-13) are arranged on the lower end face of the base (1-4) and concentrically arranged with the accommodating opening (1-12).

3. The apparatus for producing a sterile environment for tissue culture of grape according to claim 2, wherein: the diameter of the accommodating opening (1-12) is smaller than or equal to the diameter of the inner ring of the annular cushion (1-13).

4. A device for manufacturing a sterile environment for tissue culture of grapes according to claim 2 or 3, wherein: the pressing exhaust shell mechanism (1) further comprises a rubber ring (1-18), wherein the rubber ring (1-18) is fixedly arranged at the edge of the lower end face of the upper shell (1-1) and has the same diameter as the outer circle diameter of the compression pipe (1-3).

5. The apparatus for producing a sterile environment for tissue culture of grape according to claim 4, wherein: the pressing exhaust shell mechanism (1) further comprises a shaft-bearing impeller (1-15) and a driving motor (1-16), the shaft-bearing impeller (1-15) is rotatably connected to the side wall of the upper shell (1-1) and located under the communicating pipe orifice (1-17), the impeller of the shaft-bearing impeller (1-15) is located in the upper shell (1-1) and forms air flow towards the direction of the rotary filter element (1-14) when rotating, the rotating end of the driving motor (1-16) is connected with the shaft-bearing impeller (1-15), and the driving motor (1-16) is fixedly connected to the outer wall of the upper shell (1-1).

6. The apparatus for producing a sterile environment for tissue culture of grape according to claim 1, wherein: the linkage liquid draining limiting mechanism (2) comprises a connecting rod (2-1), a movable supporting seat (2-2), a limiting sliding groove (2-3), a pressing seat (2-4), a piston (2-5) with a rod, a connecting column (2-6), a spring (2-7) and a triangular section limiting block (2-8), one end of the connecting rod (2-1) is hinged on the side wall of the upper shell (1-1) and is positioned right below a rotary filter element (1-14), the other end of the connecting rod (2-1) is hinged on the movable supporting seat (2-2), the movable supporting seat (2-2) is provided with the limiting sliding groove (2-3), the pressing seat (2-4) is slidably arranged in the limiting sliding groove (2-3), the lower end face of the pressing seat (2-4) is provided with the connecting column (2-6), the connecting column (2-6) slidably penetrates through the movable supporting seat (2-2) and is connected to the upper end face of the triangular section limiting block (2-8), the spring (2-7) is sleeved on the upper end face of the connecting column (2-6) and the lower end face of the triangular section limiting block (2-8) respectively, the movable supporting seat (2-2) is characterized in that two T-shaped sliding blocks are arranged at the lower end of the movable supporting seat (2-2), the two T-shaped sliding blocks are arranged in the sliding grooves (1-5) in a one-to-one corresponding sliding mode, the triangular section limiting blocks (2-8) are matched with the triangular section limiting grooves (1-6), and the piston (2-5) with the rod is connected to one side, far away from the upper shell (1-1), of the side wall of the movable supporting seat (2-2).

7. The apparatus for producing a sterile environment for tissue culture of grape according to claim 6, wherein: the injection transfer mechanism (3) comprises a pressure change cylinder (3-2), a liquid spraying pipe (3-3), a liquid inlet pipe (3-4), a liquid inlet one-way valve (3-5) and a liquid outlet one-way valve (3-6), a rod of the rod piston (2-5) penetrates through the pressure change cylinder (3-2) in a sliding mode and is connected to a piston of the rod piston (2-5), the piston of the rod piston (2-5) is arranged in the pressure change cylinder (3-2) in a sliding mode, the liquid spraying pipe (3-3) and the liquid inlet pipe (3-4) are communicated to the pressure change cylinder (3-2), the liquid inlet one-way valve (3-5) is arranged in the liquid inlet pipe (3-4), and the liquid outlet one-way valve (3-6) is arranged in the liquid spraying pipe (3-3).

8. The apparatus for producing a sterile environment for tissue culture of grape according to claim 7, wherein: the injection transfer mechanism (3) further comprises a cylinder support (3-1), and the pressure change cylinder (3-2) is connected to the plate body (1-19) through the cylinder support (3-1).

9. The apparatus for producing a sterile environment for tissue culture of grape according to claim 8, wherein: the molecular removing mechanism (4) comprises a telescopic hose (4-1), a coiled pipe (4-2), a serpentine pipe rack (4-3), an injection pipe (4-4), an inlet pipe (4-5) and an air inlet one-way valve (4-6), one end of the telescopic hose (4-1) is communicated with an opening (1-10), the other end of the telescopic hose is communicated with the outlet end of the serpentine pipe (4-2), the inlet end of the serpentine pipe (4-2) is communicated with the outlet end of the injection pipe (4-4), the inlet end of the injection pipe (4-4) is provided with the inlet pipe (4-5) and the air inlet one-way valve (4-6) is arranged in the inlet pipe (4-5), and the serpentine pipe (4-2) is connected with the plate body (1-19) through the serpentine pipe rack (4-3).

10. The apparatus for manufacturing a sterile environment for tissue culture of grapes according to claim 9, wherein: the ejector tube (4-4) comprises a first horizontal square tube, an inclined square tube and a second horizontal square tube which are sequentially communicated, the first horizontal square tube is communicated with a coiled tube (4-2), the second horizontal square tube is communicated with an inlet tube (4-5), the first horizontal square tube is higher than the second horizontal square tube in height, and a liquid spraying tube (3-3) is connected with the second horizontal square tube and extends into the second horizontal square tube, and the mouth of the liquid spraying tube faces one side of the inclined square tube.