CN111500442A - T cell culture equipment for clinical application of cellular immunotherapy - Google Patents
T cell culture equipment for clinical application of cellular immunotherapy Download PDFInfo
- Publication number
- CN111500442A CN111500442A CN202010297247.XA CN202010297247A CN111500442A CN 111500442 A CN111500442 A CN 111500442A CN 202010297247 A CN202010297247 A CN 202010297247A CN 111500442 A CN111500442 A CN 111500442A
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- pipe
- rotating shaft
- hollow rotating
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000001744 T-lymphocyte Anatomy 0.000 title claims abstract description 33
- 238000004113 cell culture Methods 0.000 title claims abstract description 23
- 238000009169 immunotherapy Methods 0.000 title claims abstract description 21
- 230000001413 cellular effect Effects 0.000 title claims abstract description 20
- 238000005070 sampling Methods 0.000 claims abstract description 47
- 238000007789 sealing Methods 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 239000007921 spray Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 210000004027 cell Anatomy 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 3
- 238000010030 laminating Methods 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 230000006872 improvement Effects 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- 239000007789 gas Substances 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000002659 cell therapy Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000006213 oxygenation reaction Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 239000002912 waste gas Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 230000036284 oxygen consumption Effects 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a T cell culture device for clinical application of cellular immunotherapy, which belongs to the field of cellular immunotherapy and comprises a culture tank, wherein the culture tank is provided with a cover plate, the cover plate is provided with a sampling structure, a second sealing bearing and a straight pipe, the second sealing bearing is provided with a hollow rotating shaft, the hollow rotating shaft is provided with a driven gear ring, the hollow rotating shaft is provided with a first sealing bearing, the first sealing bearing is provided with an air outlet pipe, the air outlet pipe is provided with a third one-way valve, the air outlet pipe is connected with a fan, the fan is connected with a filter box, the filter box is internally provided with a filter plate, the hollow rotating shaft is provided with a stirring plate, the hollow rotating shaft is provided with a spray pipe, the straight pipe is provided with an exhaust pipe, the exhaust, the fan and the filter box are both connected with the cover plate, the driving motor is provided with a driving gear ring, the culture tank is provided with a liquid outlet pipe, and the liquid outlet pipe is fixedly connected with a control valve.
Description
Technical Field
The invention relates to the field of cellular immunotherapy, in particular to T cell culture equipment for clinical application of cellular immunotherapy.
Background
In recent years, various cell technologies such as an immunocytotherapy technology have been rapidly developed. In the next half of 2017, the FDA in the United states successively approves two types of T-therapies to be listed, which indicates that human beings make a major breakthrough in the field of cell therapy. At present, the development of cellular immunotherapy in China also enters the expressway. In 2017, 12 and 22 months, the State food and drug administration sets up 'cell therapy product research and evaluation technology guide principle trial' and issues the trial, so that the development opportunity of the immune cell therapy technology needs to be mastered urgently, the development opportunity occupies the highest point in the field of standardized T cell culture devices, and the innovation-driven development process of the biomedical industry in China is promoted.
The existing T cell culture device is single, after oxygen consumption is finished, oxygen cannot be filled into the culture solution, so that the cells inside the culture solution die easily, and the sampling is needed to be opened when the sampling detection is carried out in the T cell culture process, so that the environment unbalance or the T cells in a culture bottle are easily caused to be polluted, and the culture failure of the T cells is caused.
Based on this, the invention provides a T cell culture device for clinical application of cellular immunotherapy, which solves the technical problems.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, an object of the present invention is to provide a T cell culture apparatus for clinical application of cellular immunotherapy, which is mainly used to solve the problems that an existing T cell culture apparatus is single, after oxygen consumption is completed, oxygenation cannot be performed on the inside of a culture solution, so that internal cells are easy to die, and when sampling detection is performed during T cell culture, a cover needs to be opened to perform sampling, so that environmental imbalance in a culture bottle is easily caused or T cells are polluted, so that T cells are lost in culture.
The invention is realized by the following technical scheme: a T cell culture device for clinical application of cellular immunotherapy comprises a culture tank, a driving motor and a fan, wherein the top of the culture tank is fixedly connected with a cover plate through bolts, a sampling structure, a second sealing bearing and a straight pipe are fixedly connected with a through hole at the top of the cover plate, the second sealing bearing is fixedly connected with a hollow rotating shaft, a driven gear ring is fixedly connected onto the hollow rotating shaft, a first sealing bearing is fixedly connected onto the top of the hollow rotating shaft, an air outlet pipe is fixedly connected onto the first sealing bearing, a third one-way valve is fixedly connected onto the air outlet pipe, the air outlet pipe is fixedly connected with the output end of the fan, the input end of the fan is fixedly connected with a filter box through a pipeline, a filter plate is arranged in the filter box, an air inlet pipe is fixedly connected onto the through hole at the left end of the filter box, and, the utility model discloses a cavity pivot bottom through-hole department is along the even fixedly connected with spray tube of circumferencial direction, through-hole department fixedly connected with blast pipe on the straight tube, blast pipe fixedly connected with fifth check valve, the straight tube is at the fixedly connected with fourth check valve of blast pipe top department, straight tube top fixedly connected with and external power culture medium fixed connection's liquid feeding pipe, driving motor, fan and rose box all with apron fixed connection, driving motor's output fixedly connected with and driven gear ring meshing's initiative ring gear, tank bottom through-hole department fixedly connected with drain pipe, fixedly connected with control valve on the drain pipe.
As a further improvement of the proposal, a sealing gasket is arranged between the cover plate and the culture tank.
As the further improvement of above-mentioned scheme, sampling structure includes push rod, piston, needle tubing, goes out appearance pipe, first check valve, second check valve and sampling tube, sampling tube and apron fixed connection, fixedly connected with second check valve on the sampling tube, the fixedly connected with of sampling tube department above the second check valve goes out the appearance pipe, the first check valve of fixedly connected with on the outlet duct, sampling tube top fixedly connected with needle tubing, laminating sliding connection has the piston in the needle tubing, piston top fixedly connected with and needle tubing sliding connection's push rod.
As a further improvement of the scheme, the piston is made of rubber materials, and the sampling tube is arranged on the inner wall of the culture tank.
As a further improvement of the scheme, a plurality of groups of filter plates are arranged in the filter box, and the filter box is fixedly connected with a top cover through bolts.
As a further improvement of the scheme, the distance between the bottom of the spray pipe and the bottom in the culture tank is controlled to be 1-2 cm.
As a further improvement of the scheme, the bottom of the liquid outlet pipe is fixedly connected with a U-shaped water seal pipe, and the water outlet end of the U-shaped water seal pipe is fixedly connected with a transverse pipe.
As a further improvement of the scheme, the driving motor and the fan are both electrically connected with an external power supply.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention drives the driving gear ring to rotate through the driving motor, the driving gear ring drives the driven gear ring to rotate, the driven gear ring drives the hollow rotating shaft to rotate, the hollow rotating shaft drives the stirring plate to rotate, the stirring plate stirs the culture solution in the culture tank, the cells are in full contact with the culture solution, the propagation and growth of T cells are promoted, the culture efficiency of the T cells is improved, meanwhile, the fan pumps the air in the filter tank, the outside air enters the filter tank, the filter plate in the filter tank filters the air, the clean power air enters the air outlet pipe to push the third one-way valve to open and then enters the hollow rotating shaft, finally, the clean power air is sprayed out through the spray pipe, the oxygen is conveniently supplemented in the culture tank, the oxygenation of the interior of the culture solution is ensured, the survival rate of the cells is ensured, the waste gas pushes the fifth one-way valve to open, the waste gas is discharged from the, the comprehensiveness of oxygen supply is improved;
2. according to the invention, the push rod of the sampling structure drives the piston to move upwards, the piston moves upwards to drive the needle tube to be at negative pressure, the second one-way valve is opened, the first one-way valve is closed, the sampling tube extracts a sample in the culture tank to enter the needle tube, then the piston is pushed to move downwards, the second one-way valve is closed, the first one-way valve is opened, and the sample in the needle tube is discharged through the sample discharge tube, so that the device is convenient to sample, and when sampling and non-sampling are carried out, the sampling structure is always in a closed structure, so that outside air can be prevented from directly entering the culture tank, and pollution can be;
3. according to the invention, the control valve is designed on the liquid outlet pipe, and the U-shaped water seal pipe is arranged on the liquid outlet pipe, so that external air can be prevented from entering the culture tank from the bottom.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a front view of the structure of the present invention;
FIG. 2 is a rear view of the structure of the present invention;
FIG. 3 is a rear end view of the structure of the present invention;
FIG. 4 is a structural cross-sectional view of the present invention;
FIG. 5 is a schematic view of a sampling structure and its connection structure of the present invention;
the reference numerals are as follows:
1. the culture tank 2, the sealing gasket 3, the cover plate 4, the driving motor 5, the sampling structure 51, the push rod 52, the piston 53, the needle tube 54, the sample outlet pipe 55, the first one-way valve 56, the second one-way valve 57, the sampling pipe 6, the driving gear ring 7, the fan 8, the filter box 9, the top cover 10, the third one-way valve 11, the gas outlet pipe 12, the first sealing bearing 13, the driven gear ring 14, the liquid adding pipe 15, the hollow rotating shaft 16, the fourth one-way valve 17, the gas outlet pipe 18, the straight pipe 19, the second bearing 20, the fifth one-way valve 21, the control valve 22, the liquid outlet pipe 23, the transverse pipe 24, the U-shaped water sealing pipe 25, the spray pipe 26, the filter plate.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The technical scheme of the invention is further explained by combining the attached drawings.
Example 1
As shown in figures 1-5, a T cell culture device for clinical application of cellular immunotherapy comprises a culture tank 1, a driving motor 4 and a fan 7, wherein the top of the culture tank 1 is fixedly connected with a cover plate 3 through bolts, a sealing gasket 2 is placed between the cover plate 3 and the culture tank 1, a sampling structure 5 is fixedly connected with a through hole on the top of the cover plate 3, a second sealing bearing 19 and a straight pipe 18, the second sealing bearing 19 is fixedly connected with a hollow rotating shaft 15, a driven gear ring 13 is fixedly connected with the hollow rotating shaft 15, a first sealing bearing 12 is fixedly connected with the top of the hollow rotating shaft 15, an air outlet pipe 11 is fixedly connected with the first sealing bearing 12, a third one-way valve 10 is fixedly connected with the air outlet pipe 11, the air outlet pipe 11 is fixedly connected with the output end of the fan 7, the input end of the fan 7 is connected with a filter box 7 through a pipeline, a filter plate 26 is arranged, a plurality of groups of filter plates 26 are arranged in the filter box 7, the filter box 7 is fixedly connected with a top cover 9 through bolts, the outer wall of the hollow rotating shaft 15 is uniformly and fixedly connected with a stirring plate 27 along the circumferential direction, the through hole at the bottom of the hollow rotating shaft 15 is uniformly and fixedly connected with a spray pipe 25 along the circumferential direction, the distance between the bottom of the spray pipe 25 and the bottom in the culture tank 1 is controlled to be 1-2cm, the through hole on the straight pipe 18 is fixedly connected with an exhaust pipe 17, the exhaust pipe 17 is fixedly connected with a fifth one-way valve 20, the straight pipe 18 is fixedly connected with a fourth one-way valve 16 above the exhaust pipe 17, the top of the straight pipe 18 is fixedly connected with a liquid feeding pipe 14 fixedly connected with an external dynamic culture medium, the driving motor 4, the fan 7 and the filter box 7 are all fixedly connected with the cover plate 3, the output end, the liquid outlet pipe 22 is fixedly connected with a control valve 21, the driving gear ring 6 is driven to rotate by the driving motor 4, the driving gear ring 6 drives the driven gear ring 13 to rotate, the driven gear ring 13 drives the hollow rotating shaft 15 to rotate, the hollow rotating shaft 15 drives the stirring plate 27 to rotate, the stirring plate 27 stirs the culture solution in the culture tank 1, cells are fully contacted with the culture solution, propagation and growth of T cells are promoted, the culture efficiency of the T cells is improved, meanwhile, the fan 7 pumps the air in the filter tank 8, the outside air enters the filter tank 8, the filter plate 26 in the filter tank 8 filters the air, clean power air enters the air outlet pipe 1 to push the third one-way valve 10 to open and then enters the hollow rotating shaft 15, and finally is sprayed out through the spray pipe 25, oxygen is conveniently supplemented into the culture tank 1, oxygen charging inside of the culture solution is ensured, and the survival rate of the cells is ensured, the exhaust gas pushes the fifth check valve 20 to open, the exhaust gas is discharged from the exhaust pipe 17, and in addition, the rotating nozzle 25 helps the sprayed air to be uniformly sprayed into the culture tank 1, and the comprehensiveness of the oxygen supply is improved.
Example 2
Example 2 is a further modification to example 1.
As shown in figures 1-5, a T cell culture device for clinical application of cellular immunotherapy comprises a culture tank 1, a driving motor 4 and a fan 7, wherein the driving motor 4 and the fan 7 are both electrically connected with an external power supply, the top of the culture tank 1 is fixedly connected with a cover plate 3 through bolts, a sampling structure 5, a second sealing bearing 19 and a straight pipe 18 are fixedly connected with a through hole on the top of the cover plate 3, the second sealing bearing 19 is fixedly connected with a hollow rotating shaft 15, a driven gear ring 13 is fixedly connected on the hollow rotating shaft 15, a first sealing bearing 12 is fixedly connected on the top of the hollow rotating shaft 15, an air outlet pipe 11 is fixedly connected with the first sealing bearing 12, a third one-way valve 10 is fixedly connected on the air outlet pipe 11, the air outlet pipe 11 is fixedly connected with an output end of the fan 7, an input end of the fan 7 is connected with a filter box 7 through a pipeline, a filter plate 26 is, the outer wall of the hollow rotating shaft 15 is uniformly and fixedly connected with a stirring plate 27 along the circumferential direction, the through hole at the bottom of the hollow rotating shaft 15 is uniformly and fixedly connected with a spray pipe 25 along the circumferential direction, the through hole on the straight pipe 18 is fixedly connected with an exhaust pipe 17, the exhaust pipe 17 is fixedly connected with a fifth one-way valve 20, the straight pipe 18 is fixedly connected with a fourth one-way valve 16 above the exhaust pipe 17, the top of the straight pipe 18 is fixedly connected with a liquid feeding pipe 14 fixedly connected with an external dynamic culture medium, a driving motor 4, a fan 7 and a filter box 7 are all fixedly connected with a cover plate 3, the output end of the driving motor 4 is fixedly connected with a driving gear ring 6 meshed with a driven gear ring 13, the through hole at the bottom of the culture tank 1 is fixedly connected with a liquid outlet pipe 22, the liquid outlet pipe 22 is fixedly connected with a control valve 21, the bottom, a control valve 21 is designed on the liquid outlet pipe 22, and a U-shaped water seal pipe 24 is arranged on the liquid outlet pipe 22, so that the outside air can be prevented from entering the culture tank 1 from the bottom.
Example 3
Example 3 is a further modification to example 1.
As shown in figures 1-5, a T cell culture device for clinical application of cellular immunotherapy comprises a culture tank 1, a driving motor 4 and a fan 7, wherein the top of the culture tank 1 is fixedly connected with a cover plate 3 through a bolt, a sampling structure 5, a second sealing bearing 19 and a straight pipe 18 are fixedly connected with a through hole at the top of the cover plate 3, the sampling structure 5 comprises a push rod 51, a piston 52, a needle tube 53, a sample outlet tube 54, a first check valve 55, a second check valve 56 and a sampling tube 57, the sampling tube 57 is fixedly connected with the cover plate 3, the sampling tube 57 is fixedly connected with the second check valve 56, the sampling tube 57 is fixedly connected with the sample outlet tube 54 above the second check valve 56, a first check valve 55 is fixedly connected with the top of the sampling tube 57, the needle tube 53 is fixedly connected with the top of the needle tube 53, the piston 52 is slidably connected with the top of the needle tube 53, the push rod 51 is fixedly connected with the, the piston 52 is made of rubber materials, the sampling tube 57 is arranged at the inner wall of the culture tank 1, the second sealing bearing 19 is fixedly connected with the hollow rotating shaft 15, the hollow rotating shaft 15 is fixedly connected with the driven gear ring 13, the top of the hollow rotating shaft 15 is fixedly connected with the first sealing bearing 12, the first sealing bearing 12 is fixedly connected with the air outlet tube 11, the air outlet tube 11 is fixedly connected with the third one-way valve 10, the air outlet tube 11 is fixedly connected with the output end of the fan 7, the input end of the fan 7 is connected with the filter box 7 through a pipeline, a filter plate 26 is arranged in the filter box 7, the left end through hole of the filter box 7 is fixedly connected with an air inlet tube, the outer wall of the hollow rotating shaft 15 is uniformly and fixedly connected with the stirring plate 27 along the circumferential direction, the through hole at the bottom of the hollow rotating shaft 15 is uniformly and fixedly connected with, a fourth one-way valve 16 is fixedly connected to the top of the straight pipe 18 above the exhaust pipe 17, a liquid feeding pipe 14 fixedly connected with an external dynamic culture medium is fixedly connected to the top of the straight pipe 18, a driving motor 4, a fan 7 and a filter box 7 are all fixedly connected with the cover plate 3, an output end of the driving motor 4 is fixedly connected with a driving gear ring 6 meshed with a driven gear ring 13, a liquid outlet pipe 22 is fixedly connected to a through hole at the bottom of the culture tank 1, a control valve 21 is fixedly connected to the liquid outlet pipe 22, a piston 52 is driven to move upwards by a push rod 51 of the sampling structure 5, the piston 52 moves upwards to drive a needle tube 53 to be at negative pressure, a second one-way valve 56 is opened, a first one-way valve 55 is closed, a sampling pipe 57 extracts a sample in the culture tank 1 to enter the needle tube 53, then the piston 52 is driven to move downwards, the second one-way valve 56 is closed, the, make the device conveniently take a sample, when taking a sample and not taking a sample, sample structure 5 is in the enclosed construction all the time, can avoid the air of outside directly to get into in culture tank 1, can avoid causing the pollution.
The specific use is as follows: the driving motor 4 is started, the driving motor 4 drives the driving gear ring 6 to rotate, the driving gear ring 6 drives the driven gear ring 13 to rotate, the driven gear ring 13 drives the hollow rotating shaft 15 to rotate, the hollow rotating shaft 15 drives the stirring plate 27 to rotate, the stirring plate 27 stirs the culture solution in the culture tank 1, the cells are fully contacted with the culture solution, the propagation and growth of the T cells are promoted, the culture efficiency of the T cells is improved, meanwhile, the fan 7 pumps the air in the filter tank 8, the outside air enters the filter tank 8, the filter plate 26 in the filter tank 8 filters the air, the clean power air enters the air outlet pipe 1 to push the third one-way valve 10 to be opened, then enters the hollow rotating shaft 15 and finally is sprayed out through the spray pipe 25, oxygen is conveniently supplemented into the culture tank 1, the oxygenation inside of the culture solution is ensured, the survival rate of the cells is ensured, the waste gas pushes the fifth one-way, the exhaust gas is discharged from the exhaust pipe 17, and the rotating nozzle 25 helps the sprayed air to be uniformly sprayed into the culture tank 1, thereby improving the comprehensiveness of the oxygen supply. When sampling is needed, the push rod 51 of the sampling structure 5 drives the piston 52 to move upwards, the piston 52 moves upwards to drive the needle tube 53 to be at negative pressure, the second one-way valve 56 is opened, the first one-way valve 55 is closed, the sampling tube 57 extracts a sample in the culture tank 1 to enter the needle tube 53, then the piston 52 is pushed to move downwards, the second one-way valve 56 is closed, the first one-way valve 55 is opened, the sample in the needle tube 53 is discharged through the sample outlet tube, so that the device is convenient to sample, and when sampling and non-sampling are carried out, the sampling structure 5 is always in a closed structure, so that outside air can be prevented from directly entering the culture tank 1, and pollution can be avoided; when changing the culture solution, the culture solution passes through drain pipe 22, U-shaped water seal pipe 24 and violently manages 23 discharges, simultaneously, starts external power culture medium, and external power culture medium passes through pressure promotion fourth check valve 16 and opens, and the culture medium passes through in liquid feeding pipe 14 gets into culture tank 1, conveniently changes the culture medium, design control valve 21 on the drain pipe 22, is equipped with U-shaped water seal pipe 24 on the drain pipe 22 simultaneously, can block external air and get into culture tank 1 from the bottom in.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. A T cell culture equipment of cell immunotherapy clinical application, includes culture tank (1), driving motor (4) and fan (7), its characterized in that: the device is characterized in that the top of the culture tank (1) is fixedly connected with a cover plate (3) through a bolt, a sampling structure (5), a second sealing bearing (19) and a straight pipe (18) are fixedly connected with a through hole at the top of the cover plate (3), the second sealing bearing (19) is fixedly connected with a hollow rotating shaft (15), a driven gear ring (13) is fixedly connected onto the hollow rotating shaft (15), a first sealing bearing (12) is fixedly connected onto the top of the hollow rotating shaft (15), an air outlet pipe (11) is fixedly connected onto the first sealing bearing (12), a third one-way valve (10) is fixedly connected onto the air outlet pipe (11), the air outlet pipe (11) is fixedly connected with the output end of a fan (7), the input end of the fan (7) passes through a pipeline and a filter box (7), a filter plate (26) is arranged in the filter box (7), and an air inlet pipe is fixedly connected, the device is characterized in that a stirring plate (27) is uniformly and fixedly connected to the outer wall of the hollow rotating shaft (15) along the circumferential direction, a spray pipe (25) is uniformly and fixedly connected to the bottom through hole of the hollow rotating shaft (15) along the circumferential direction, an exhaust pipe (17) is fixedly connected to the upper through hole of the straight pipe (18), a fifth one-way valve (20) is fixedly connected to the exhaust pipe (17), a fourth one-way valve (16) is fixedly connected to the upper portion of the straight pipe (18), a liquid feeding pipe (14) fixedly connected with an external dynamic culture medium is fixedly connected to the top of the straight pipe (18), a driving motor (4), a fan (7) and a filter box (7) are fixedly connected with a cover plate (3), a driving gear ring (6) meshed with a driven gear ring (13) is fixedly connected to the output end of the driving motor (4), and a liquid outlet pipe (22) is fixedly, and the liquid outlet pipe (22) is fixedly connected with a control valve (21).
2. A T cell culture apparatus for clinical application of cellular immunotherapy according to claim 1, characterized in that: a sealing gasket (2) is arranged between the cover plate (3) and the culture tank (1).
3. A T cell culture apparatus for clinical application of cellular immunotherapy according to claim 1, characterized in that: sampling structure (5) are including push rod (51), piston (52), needle tubing (53), go out appearance pipe (54), first check valve (55), second check valve (56) and sampling tube (57), sampling tube (57) and apron (3) fixed connection, fixedly connected with second check valve (56) are gone up in sampling tube (57), fixedly connected with goes out appearance pipe (54) is located to fixedly connected with above second check valve (56) sampling tube (57), first check valve (55) of fixedly connected with on outlet duct (11), sampling tube (57) top fixedly connected with needle tubing (53), laminating sliding connection has piston (52) in needle tubing (53), piston (52) top fixedly connected with and needle tubing (53) sliding connection's push rod (51).
4. A T cell culture apparatus for clinical application of cellular immunotherapy according to claim 1, characterized in that: the piston (52) is made of rubber materials, and the sampling tube (57) is arranged on the inner wall of the culture tank (1).
5. A T cell culture apparatus for clinical application of cellular immunotherapy according to claim 1, characterized in that: a plurality of groups of filter plates (26) are arranged in the filter box (7), and the filter box (7) is fixedly connected with a top cover (9) through bolts.
6. A T cell culture apparatus for clinical application of cellular immunotherapy according to claim 1, characterized in that: the distance between the bottom of the spray pipe (25) and the bottom in the culture tank (1) is controlled to be 1-2 cm.
7. A T cell culture apparatus for clinical application of cellular immunotherapy according to claim 1, characterized in that: the bottom of the liquid outlet pipe (22) is fixedly connected with a U-shaped water seal pipe (24), and the water outlet end of the U-shaped water seal pipe (24) is fixedly connected with a transverse pipe (23).
8. A T cell culture apparatus for clinical application of cellular immunotherapy according to claim 5, wherein: the driving motor (4) and the fan (7) are electrically connected with an external power supply.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010297247.XA CN111500442A (en) | 2020-04-15 | 2020-04-15 | T cell culture equipment for clinical application of cellular immunotherapy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010297247.XA CN111500442A (en) | 2020-04-15 | 2020-04-15 | T cell culture equipment for clinical application of cellular immunotherapy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111500442A true CN111500442A (en) | 2020-08-07 |
Family
ID=71865886
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010297247.XA Pending CN111500442A (en) | 2020-04-15 | 2020-04-15 | T cell culture equipment for clinical application of cellular immunotherapy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111500442A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112748223A (en) * | 2020-12-08 | 2021-05-04 | 福州东星生物技术有限公司 | Device and method for detecting residual quantity of sulfur dioxide in edible fungi |
| CN115193379A (en) * | 2022-07-30 | 2022-10-18 | 辽宁索能环保能源科技有限公司 | Organic solid waste reaction kettle |
| CN117125813A (en) * | 2023-10-08 | 2023-11-28 | 武汉格林环源净化工程有限公司 | Device and method for strengthening activated sludge in aerobic tank |
| CN118460372A (en) * | 2024-07-09 | 2024-08-09 | 山东德瑞克仪器股份有限公司 | Stem cell culture device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206654913U (en) * | 2017-04-14 | 2017-11-21 | 青岛瑞思德生物科技有限公司 | A kind of separation device for adipose-derived stem cells |
| CN109401953A (en) * | 2018-11-20 | 2019-03-01 | 湖州铭城科技有限公司 | Facilitate the soy sauce fermenting apparatus of sample detection |
| CN209024547U (en) * | 2018-08-03 | 2019-06-25 | 中享生命科技有限公司 | A kind of cell culture apparatus |
| CN209081909U (en) * | 2018-09-11 | 2019-07-09 | 华夏源(上海)干细胞科技有限公司 | A kind of biotechnology continues culture apparatus with cell |
| CN110468024A (en) * | 2019-08-14 | 2019-11-19 | 中国人民解放军第四军医大学 | Bacterium extraction element |
| CN209854174U (en) * | 2018-11-13 | 2019-12-27 | 广西医科大学第一附属医院 | Cell culture device for biotechnology |
-
2020
- 2020-04-15 CN CN202010297247.XA patent/CN111500442A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206654913U (en) * | 2017-04-14 | 2017-11-21 | 青岛瑞思德生物科技有限公司 | A kind of separation device for adipose-derived stem cells |
| CN209024547U (en) * | 2018-08-03 | 2019-06-25 | 中享生命科技有限公司 | A kind of cell culture apparatus |
| CN209081909U (en) * | 2018-09-11 | 2019-07-09 | 华夏源(上海)干细胞科技有限公司 | A kind of biotechnology continues culture apparatus with cell |
| CN209854174U (en) * | 2018-11-13 | 2019-12-27 | 广西医科大学第一附属医院 | Cell culture device for biotechnology |
| CN109401953A (en) * | 2018-11-20 | 2019-03-01 | 湖州铭城科技有限公司 | Facilitate the soy sauce fermenting apparatus of sample detection |
| CN110468024A (en) * | 2019-08-14 | 2019-11-19 | 中国人民解放军第四军医大学 | Bacterium extraction element |
Non-Patent Citations (1)
| Title |
|---|
| 陈艳 等, 中国铁道出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112748223A (en) * | 2020-12-08 | 2021-05-04 | 福州东星生物技术有限公司 | Device and method for detecting residual quantity of sulfur dioxide in edible fungi |
| CN112748223B (en) * | 2020-12-08 | 2023-08-29 | 福州东星生物技术有限公司 | Device and method for detecting residual quantity of sulfur dioxide in edible fungi |
| CN115193379A (en) * | 2022-07-30 | 2022-10-18 | 辽宁索能环保能源科技有限公司 | Organic solid waste reaction kettle |
| CN117125813A (en) * | 2023-10-08 | 2023-11-28 | 武汉格林环源净化工程有限公司 | Device and method for strengthening activated sludge in aerobic tank |
| CN117125813B (en) * | 2023-10-08 | 2024-02-06 | 武汉格林环源净化工程有限公司 | Device and method for strengthening activated sludge in aerobic tank |
| CN118460372A (en) * | 2024-07-09 | 2024-08-09 | 山东德瑞克仪器股份有限公司 | Stem cell culture device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111500442A (en) | T cell culture equipment for clinical application of cellular immunotherapy | |
| CN207446943U (en) | Anaerobic Digestion of Municipal Solid Waste processing equipment | |
| CN111875092B (en) | Fishery breeding tail water treatment device | |
| CN213739228U (en) | Fermentation cylinder for fertilizer | |
| CN206321482U (en) | A kind of natural bioremediation gas collecting apparatus | |
| CN206483313U (en) | The equipment that organic exhaust gas is handled using micro-nano bubble | |
| CN215742821U (en) | Waste gas treatment device for thermal power plant | |
| CN206901983U (en) | A kind of efficient non-energy consumption device for treating domestic sewage | |
| CN212790691U (en) | Loading attachment is used on automobile exhaust sanitizer production line | |
| CN212731529U (en) | Multifunctional air purification equipment | |
| CN211570493U (en) | Organic fertilizer fermentation device | |
| CN103387934A (en) | Full-mechanical drive feeding and sampling device of reactor with high solid concentration | |
| CN109012066B (en) | Organic waste gas biological treatment's collection device | |
| CN207713741U (en) | Methane-generating pit based on biogas anaerobic fermentation technology | |
| CN207330607U (en) | A kind of sewage disposal system using photocatalysis treatment | |
| CN205133223U (en) | A aeration equipment that is used for gravel, and contact is technological within a definite time | |
| CN219526413U (en) | Sewage treatment device utilizing biological deodorization | |
| CN220376541U (en) | Sludge aerobic fermentation waste gas treatment equipment | |
| CN220182825U (en) | Medical sewage treatment integrated device | |
| CN212607374U (en) | Microorganism hanging material rotating device | |
| CN212356924U (en) | Hydraulic engineering sewage treatment circulating device | |
| CN211913251U (en) | Environment-friendly exhaust treatment device for reaction system of waterproof coiled material | |
| CN114031161B (en) | Reclaimed water recycling treatment equipment for domestic sewage | |
| CN108410703A (en) | A kind of efficient ferment apparatus | |
| CN220834231U (en) | Purification device for N-acetylneuraminic acid processing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200807 |