CN108300713A - The method and device of fixed cell - Google Patents
The method and device of fixed cell Download PDFInfo
- Publication number
- CN108300713A CN108300713A CN201711493770.4A CN201711493770A CN108300713A CN 108300713 A CN108300713 A CN 108300713A CN 201711493770 A CN201711493770 A CN 201711493770A CN 108300713 A CN108300713 A CN 108300713A
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- China
- Prior art keywords
- cell
- crystal material
- micro crystal
- fixed cell
- container
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Immunology (AREA)
- Clinical Laboratory Science (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of method and device of fixed cell, the method for the fixed cell is：Using micro crystal material adherent cell, to the fixed cell.The present invention has many advantages, such as that adsorption capacity is strong, wear-resistant, usage time is long, small to impact cell.
Description
Technical field
The present invention relates to cells to fix, more particularly to the method and device of fixed cell.
Background technology
Cell fixation at present possesses various ways, absorption method, investment, covalent coupling method, cross-linking method etc.；Using novel micro-
Brilliant material it is targeted be absorption method.Absorption method is so that cell is adsorbed on the surface of carrier by adsorption capacity and cell is made to fix
Method on carrier.Common fixation support has：Diatomite, cellular glass, ceramics, plastics, doughnut and wire
Net etc..Above-mentioned fixation support cell is easier to fall off, and intolerant to abrasion, is more easily damaged.
These cells, which fix material, mainly following deficiency：Material mechanical strength itself is low intolerant to abrasion；Easily by microorganism
Degradation；Short life；It is poor to the adsorption capacity of cell.
Invention content
For the deficiency for solving in above-mentioned prior art, the present invention provides a kind of fixations, small to impact cell
Fixation cell method.
The purpose of the present invention is what is be achieved through the following technical solutions：
The method of fixed cell, the method for the fixed cell are：Using micro crystal material adherent cell, described in fixed
Cell.
The present invention also aims to provide the device for realizing the above method, which is by following skill
Art scheme is achieved：
The device of fixed cell, the device of the fixed cell include：
Container, the container is for accommodating micro crystal material；
Micro crystal material, the micro crystal material are arranged in the container.
Compared with prior art, the device have the advantages that being：
1. crystallite is extremely strong to the adsorption capacity of cell, more stronger than other materials to the stationarity of cell, effectively cultivated
Cell, can be accurately positioned and operate on it when being operated to cell will not make its flowing；
Cell constantly becomes larger in growth course, and micro crystal material can voluntarily movement be close to around, and cell can be had
One good living environment；
2. high mechanical strength, wear-resistant, usage time is long；
3. being not easy to be degraded by microorganisms, can use for a long time；
4. providing a better cells survival environment, the influence to cell growth is reduced.
Description of the drawings
With reference to attached drawing, the disclosure will be easier to understand.Skilled addressee readily understands that be：This
A little attached drawings are used only for the technical solution illustrated the present invention, and are not intended to and are construed as limiting to protection scope of the present invention.
In figure：
Fig. 1 is the structure diagram of the device of fixed cell according to the ... of the embodiment of the present invention.
Specific implementation mode
Fig. 1 and following description describe the present invention optional embodiment with instruct those skilled in the art how to implement and
Reproduce the present invention.In order to instruct technical solution of the present invention, some conventional aspects are simplified or have been omitted.Those skilled in the art answer
The understanding is originated from the modification of these embodiments or replacement will within the scope of the invention.Under those skilled in the art should understand that
Stating feature can combine in various ways to form multiple modifications of the present invention.The invention is not limited in following optional as a result,
Embodiment, and be only limited by the claims and their equivalents.
Embodiment：
Fig. 1 schematically illustrates the device of the fixation cell of the embodiment of the present invention 1, as shown in Figure 1, the fixed cell
Device include：
Container 5, such as culture dish, the container is for accommodating micro crystal material；
Micro crystal material 4, the micro crystal material are entirely laid in the container；
The cell 1 is just to be placed in container 5, and the cell 2 is the cell being placed in after being cultivated 12 hours in container 5, institute
It is the cell being placed in container 5 after 24 hours to state cell 3.When cell has just been placed in container 5, the size and model of micro crystal material 2
The process grown for one such as the micro crystal material 4 around cell 1, cell 2,3 is enclosed, with the gradual increase of cell, micro crystal material 2
Diffusion movement around, and certain change in shape occurs, give cell one free growing space, to not influence cell
Normal growth.
The method of the method for the fixation cell of the embodiment of the present invention, the fixed cell is：It is inhaled naturally using micro crystal material
Attached cell, to which the fixed cell just can search out relatively when needing to operate cell again next time in fixed position
The cell answered；The micro crystal material is laid in culture dish.
Claims (5)
1. the method for fixing cell, the method for the fixed cell are：Using micro crystal material adherent cell, to fixed described thin
Born of the same parents.
2. the method for fixed cell according to claim 1, it is characterised in that：The micro crystal material is laid in culture dish
In.
3. fixing the device of cell, the device of the fixed cell includes：
Container, the container is for accommodating micro crystal material；
Micro crystal material, the micro crystal material are arranged in the container.
4. the device of fixed cell according to claim 3, it is characterised in that：The smooth shakedown of micro crystal material enters described
In container.
5. the device of fixed cell according to claim 1, it is characterised in that：The container is culture dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711493770.4A CN108300713A (en) | 2017-12-31 | 2017-12-31 | The method and device of fixed cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711493770.4A CN108300713A (en) | 2017-12-31 | 2017-12-31 | The method and device of fixed cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108300713A true CN108300713A (en) | 2018-07-20 |
Family
ID=62868035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711493770.4A Pending CN108300713A (en) | 2017-12-31 | 2017-12-31 | The method and device of fixed cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108300713A (en) |
Citations (11)
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| CN1556203A (en) * | 2003-12-31 | 2004-12-22 | 中国人民解放军军事医学科学院生物工 | Aimal cell multipore micro carrier immobilized high efficiency culturing method and its culturing medium |
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| CN103776771A (en) * | 2014-01-20 | 2014-05-07 | 浙江理工大学 | Method for detecting moso bamboo nano microcrystalline cellulose biocompatibility |
| CN105051182A (en) * | 2013-03-25 | 2015-11-11 | 赛尔卢泰奇股份有限公司 | Method and device for producing cell cultures of human or animal cells |
| CN105521525A (en) * | 2015-12-16 | 2016-04-27 | 广州创赛生物医用材料有限公司 | Porous composite scaffold for bone tissue engineering and preparation method therefor |
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| CN106606508A (en) * | 2015-10-27 | 2017-05-03 | 余秀民 | Available microcrystalline cellulose for preventing and treating cervical cancer |
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| CN1556203A (en) * | 2003-12-31 | 2004-12-22 | 中国人民解放军军事医学科学院生物工 | Aimal cell multipore micro carrier immobilized high efficiency culturing method and its culturing medium |
| CN101652471A (en) * | 2007-02-12 | 2010-02-17 | 碧奥瑞真有限责任公司 | Be used for technology and supporting structure that viable cell is cultivated |
| CN103354834A (en) * | 2010-10-27 | 2013-10-16 | 芬欧汇川集团 | Plant derived cell culture material |
| CN102864119A (en) * | 2012-09-27 | 2013-01-09 | 中国科学院苏州纳米技术与纳米仿生研究所 | Carrier for cell culturing and preparation method of carrier |
| CN105051182A (en) * | 2013-03-25 | 2015-11-11 | 赛尔卢泰奇股份有限公司 | Method and device for producing cell cultures of human or animal cells |
| US20160024462A1 (en) * | 2013-03-25 | 2016-01-28 | Xellutec Gmbh | Method and device for producing a cell culture of human or animal cells |
| CN103525801A (en) * | 2013-09-13 | 2014-01-22 | 西安交通大学 | Cell stationary phase based on microcarrier cells and preparation method of cell stationary phase |
| CN103776771A (en) * | 2014-01-20 | 2014-05-07 | 浙江理工大学 | Method for detecting moso bamboo nano microcrystalline cellulose biocompatibility |
| CN106606508A (en) * | 2015-10-27 | 2017-05-03 | 余秀民 | Available microcrystalline cellulose for preventing and treating cervical cancer |
| CN105521525A (en) * | 2015-12-16 | 2016-04-27 | 广州创赛生物医用材料有限公司 | Porous composite scaffold for bone tissue engineering and preparation method therefor |
| CN205347445U (en) * | 2016-01-22 | 2016-06-29 | 天津卫凯生物工程有限公司 | Cell culture fibre membrane support frame, cell culture support and cell culture device |
| CN106754627A (en) * | 2017-02-27 | 2017-05-31 | 南京新诺丹生物技术有限公司 | A kind of carrier for cultured cells |
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| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180720 |
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| RJ01 | Rejection of invention patent application after publication |