CN216808765U - Batch bacteria shaking plate - Google Patents
Batch bacteria shaking plate Download PDFInfo
- Publication number
- CN216808765U CN216808765U CN202220471420.8U CN202220471420U CN216808765U CN 216808765 U CN216808765 U CN 216808765U CN 202220471420 U CN202220471420 U CN 202220471420U CN 216808765 U CN216808765 U CN 216808765U
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- CN
- China
- Prior art keywords
- bacteria
- shaking
- shake
- fungus
- batch
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- Expired - Fee Related
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- 241000894006 Bacteria Species 0.000 title claims abstract description 60
- 239000004743 Polypropylene Substances 0.000 claims abstract description 6
- -1 polypropylene Polymers 0.000 claims abstract description 6
- 229920001155 polypropylene Polymers 0.000 claims abstract description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000741 silica gel Substances 0.000 claims abstract description 5
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 5
- 241000233866 Fungi Species 0.000 claims description 38
- 238000009423 ventilation Methods 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 9
- 229920000742 Cotton Polymers 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 abstract description 9
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 230000000644 propagated effect Effects 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 6
- 230000003321 amplification Effects 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 241001052560 Thallis Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000003491 array Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a batch bacteria shaking plate which comprises a bacteria shaking plate body, wherein the bacteria shaking plate body is of a cuboid structure made of polypropylene, a plurality of open cavities which are arranged in a rectangular array are arranged on the bacteria shaking plate body, each cavity forms a bacteria shaking tube, each bacteria shaking tube consists of a tube stem and a spherical bottom, and a cover plate made of silica gel is further arranged on the bacteria shaking plate body. The batch bacteria shaking plate has the following advantages: 1) the workload of shaking a large batch of single bacterial colonies is reduced, and the waste of a culture medium is reduced; 2) the spring clamp can be adapted to the traditional shaking table; 3) the spherical pipe bottom can better shake the bacteria culture solution, so that the bacteria can be well propagated and grown, the bacteria can obtain higher survival rate, the density of the cultured bacteria is increased, and the experimental efficiency is greatly improved.
Description
Technical Field
The utility model relates to a bacterium shaking plate, in particular to a batch bacterium shaking plate for molecular cloning, and belongs to the technical field of molecular biology experimental instruments.
Background
In molecular biology experiments, after a carrier is constructed by genetic engineering means and positive bacteria are screened, multiple positive bacteria are required to be inoculated at one time for amplification culture, and when multiple carriers are constructed, liquid culture media of dozens or hundreds are required to be inoculated at the same time for amplification culture. Traditional culture medium container uses the triangular flask usually, but the volume of triangular flask is too big, and aseptic workstation and shaking table space are limited, can't accomplish the simultaneous culture of a large amount of samples, and shake the fungus pipe in the fungus board in current batch and mostly be the straight tube, shake the fungus volume limited, and shake the difficulty and lead to a large amount of thalli deposits in the bottom, finally lead to the thalli to grow slowly, influence the progress of biology experiment, waste a large amount of time.
Disclosure of Invention
Aiming at the problems in the prior art, the utility model provides a batch bacteria shaking plate, and aims to solve the problem that the prior bacteria shaking plate is poor in bacteria shaking effect.
In order to achieve the purpose, the utility model adopts the technical scheme that:
the utility model provides a shake fungus board in batches, contains and shakes fungus board body, shake fungus board body and be the cuboid structure of polypropylene material it is provided with a plurality of opening cavities that are the rectangle array and arrange to shake fungus board body, and each cavity constitutes one and shakes the fungus pipe, every shakes the fungus pipe and comprises at the bottom of stem and the ball shake the apron that still is equipped with the silica gel material on the fungus board body.
Furthermore, a plurality of ventilation covers corresponding to the cavities are arranged on the lower surface of the cover plate, a ventilation hole or a ventilation pipe is arranged in the center of each ventilation cover, and a bacteria filtering membrane or cotton is arranged in each ventilation hole or each ventilation pipe.
Further, the diameter of the ventilation cover is larger than the diameter of the pipe orifice of the shake bacteria pipe.
Further, the at least two adjacent side surfaces of the bacteria shaking plate body are also provided with bacteria shaking tube coordinate mark symbols.
The beneficial effect who adopts above-mentioned scheme does:
1) the bacteria shaking plate and the bacteria shaking pipe are of an integrated structure, so that large batches of bacteria can be cultured simultaneously, the workload of shaking large batches of single bacterial colonies can be reduced, and the device can be adapted to a spring clamp of a traditional shaking table;
2) each bacteria shaking tube consists of a tube stem and a sphere bottom, the sphere bottom space can contain a culture medium, the waste of the culture medium can be reduced, meanwhile, the sphere bottom space is beneficial to the shaking of the culture medium, the sphere bottom can enable a bacteria culture solution to better shake, bacteria can well breed and grow, accordingly, the bacteria can obtain higher survival rate, the density of the cultured bacteria is increased, the experimental efficiency is greatly improved, the bacteria are not easy to deposit at the tube bottom, the bacteria grow rapidly, and the experimental progress can be remarkably improved;
3) shake the fungus board body and adopt the polypropylene material, the apron is the silica gel material, all can carry out the high temperature disinfection degerming, but experimental apparatus reuse saves the experiment cost.
Drawings
FIG. 1 is a schematic structural diagram of a batch bacteria shaking plate of the present invention.
FIG. 2 is a schematic diagram of the longitudinal section structure of the batch bacteria shaking plate of the utility model.
The mark in the figure is: shake fungus board body 1, shake fungus pipe 2, ventilative lid 3, apron 4, bleeder vent 5, shake fungus pipe coordinate mark 6, stem 7, ball end 8.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1, a shake fungus board in batches contains shake fungus board body 1, shake fungus board body 1 and be the cuboid structure of polypropylene material shake and be provided with a plurality of opening cavities that are the rectangle array and arrange on the fungus board body 1, each cavity constitutes one and shakes fungus pipe 2, has shown 4 in fig. 1 shake fungus pipe structure of 4 arrays, shake fungus board body like this and shake the structure that fungus pipe has constituteed an integral type, the operation of fungus is shaken in the cooperation of convenient and shaking table. Meanwhile, each side face of the bacteria shaking plate body 1 is provided with a bacteria shaking tube coordinate mark 6, so that the coordinates of 16 bacteria shaking tubes can be distinguished, for example, A1, A2, A3, A4, B1, B2, and D4 are convenient for experimental marking.
Shaking fungus board body 1 and being equipped with apron 4 lower surface is provided with a plurality of ventilative covers 3 that correspond with the cavity, apron and ventilative cover also formula structure as an organic whole, and its material adopts silicon rubber, and the silica gel material does benefit to sealed while and the frictional force between the polypropylene is great. The center of each ventilation cover is provided with a ventilation hole or a ventilation pipe, in the embodiment, the center of each ventilation cover is provided with a ventilation hole 5, and a bacteria filtering membrane or cotton is arranged in the ventilation hole 5. The bacteria filtering membrane or the filling cotton can isolate external microorganisms. The cover plate is used for covering and sealing the opening of the shake bacteria tube, so that the diameter of the ventilation cover is required to be larger than the diameter of the tube opening of the shake bacteria tube, and the diameter of the ventilation cover is slightly larger than the diameter of the tube opening of the shake bacteria tube by 3-5mm during actual manufacturing. The design of apron can be covered one row or shake the fungus pipe in the line, also can will shake fungus pipe array cover entirely, can select according to specific experimental operation to the experiment of convenience is the purpose. For example, the cover plate shown in fig. 1 can cover a row of shake bacteria tubes, and in order to fully cover the shake bacteria plates, four cover plates are required to be used for covering respectively, so that the experiment operation can be performed in steps, and errors are not easy to occur.
Further, the structure of the single shake bacteria tube 2 is shown in fig. 2, and the single shake bacteria tube is composed of a tube stem 7 and a bulb bottom 8. The ball end space is great, can hold the culture medium, does benefit to rocking of culture medium, and spherical socle can make better rocking of bacterial culture solution, makes good breed and growth of bacterium to make the bacterium obtain higher survival rate, increase the density of cultivateing the bacterium, make the thallus be difficult to deposit at the socle because rocking of the culture solution simultaneously, the thallus grows rapidly, has improved experimental efficiency greatly.
Shake the fungus board body 3 and can fix the tradition and have spring anchor clamps the shaking table on practical, every shakes the fungus pipe and can shakes a single colony, shakes fungus board 3 and apron 4 whole all can be high temperature resistant, can use high temperature autoclaving, uses repeatedly.
Description of the method of use of the utility model: 1) marking a plurality of positive colonies needing to be subjected to amplification culture on a transformation plate; 2) selecting a shake tube according to the number of positive colonies to be cultured, planning the coordinates of the shake tube used by each positive clone, and adding a certain amount of culture medium (not exceeding the central line of a sphere) into each tube; 3) inoculating the positive bacterial colony into a bacteria shaking tube with corresponding coordinates according to a plan, covering a plate, placing the bacteria shaking plate into a shaking table, fixing by using a proper method, and carrying out bacteria shaking culture; 4) and (5) taking out the sample after fungus shaking is finished, and cleaning a fungus shaking plate and a cover plate for the next experiment.
Claims (4)
1. The utility model provides a shake fungus board in batches contains shake fungus board body, its characterized in that: shake the fungus board body and be the cuboid structure of polypropylene material it is provided with a plurality of opening cavities that are the rectangular array and arrange to shake the fungus board body, each cavity constitutes one and shakes the fungus pipe, every shakes at the bottom of the fungus pipe by stem and bulb and constitutes shake the apron that still is equipped with the silica gel material on the fungus board body.
2. The batch bacteria shaking plate of claim 1, wherein: the lower surface of the cover plate is provided with a plurality of ventilation covers corresponding to the cavities, the center of each ventilation cover is provided with a ventilation hole or a ventilation pipe, and a bacteria filtering membrane or cotton is arranged in the ventilation hole or the ventilation pipe.
3. The batch bacteria-shaking plate of claim 2, wherein: the diameter of the ventilation cover is larger than the diameter of the pipe orifice of the shake bacteria pipe.
4. The batch bacteria-shaking plate of claim 1, wherein: and at least two adjacent side surfaces of the bacteria shaking plate body are also provided with bacteria shaking tube coordinate mark symbols.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220471420.8U CN216808765U (en) | 2022-03-07 | 2022-03-07 | Batch bacteria shaking plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220471420.8U CN216808765U (en) | 2022-03-07 | 2022-03-07 | Batch bacteria shaking plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216808765U true CN216808765U (en) | 2022-06-24 |
Family
ID=82043763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220471420.8U Expired - Fee Related CN216808765U (en) | 2022-03-07 | 2022-03-07 | Batch bacteria shaking plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216808765U (en) |
-
2022
- 2022-03-07 CN CN202220471420.8U patent/CN216808765U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220624 |
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| CF01 | Termination of patent right due to non-payment of annual fee |