CN217694756U

CN217694756U - Plant tissue culture bottle

Info

Publication number: CN217694756U
Application number: CN202221777657.5U
Authority: CN
Inventors: 李永平; 杨宝明; 王春梅; 刘立娜; 李迅东; 尹可锁; 郑泗军
Original assignee: YUNNAN DEHONG TROPICAL AGRICULTURE RESEARCH INSTITUTE; Institute of Agricultural Environment and Resources of Yunnan Academy of Agricultural Sciences
Current assignee: YUNNAN DEHONG TROPICAL AGRICULTURE RESEARCH INSTITUTE; Institute of Agricultural Environment and Resources of Yunnan Academy of Agricultural Sciences
Priority date: 2022-07-12
Filing date: 2022-07-12
Publication date: 2022-11-01
Anticipated expiration: 2032-07-12

Abstract

The utility model discloses a plant tissue culture bottle in the field of plant tissue culture, which comprises a glass bottle and a bottle cap, wherein an air hole is arranged between the central positions of the upper side wall and the lower side wall of the bottle cap, and the bottom wall of the bottle cap is positioned outside the air hole and is fixedly connected with a rubber sealing plug in a concentric circle shape; the air hole inner side wall sliding mounting has a cotton plug, the rubber sealing plug diapire is two connecting rods of symmetry fixedly connected with, connecting rod lateral wall sliding mounting has a plant constraint ring, the connecting rod bottom is provided with the culture tank. The device can realize that the plant in the plant tissue culture bottle is convenient to take out and cannot damage the plant; through the plant constraint ring that is provided with the lift design, when the plant to co-altitude cultivates, the altitude that its plant constraint ring can carry out the altitude mixture control of adaptability, is provided with the gas pocket through being provided with the bottle lid roof in addition to be provided with independent cotton plug structure in gas pocket top opening part, be provided with the filter membrane in the cotton plug structure, carry out the filter membrane and change the during operation, the convenience is good.

Description

Plant tissue culture bottle

Technical Field

The utility model relates to a plant tissue culture field especially relates to a plant tissue culture bottle.

Background

The tissue culture of plants is a new asexual propagation technology developed in recent decades based on the theory that plant cells have totipotency. Tissue culture of plants, also called isolated culture in the broad sense, refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from plant bodies, inoculating the tissues, organs or cells, protoplasts, etc. to a culture medium containing various nutrients and phytohormones under aseptic conditions for culture to obtain regenerated whole plants or to produce other products having economic value.

At present, when plants cultured in the existing culture bottles are higher, or the root systems of tissue culture seedlings are wound, the plants are not easy to take out from the culture bottles. When the tissue culture bottle is taken out of the culture bottle, the cultured seedlings are easily damaged, meanwhile, the bottle cap is provided with an air vent, the air vent is sealed by a filter membrane and a cotton plug, the filter membrane is troublesome to replace, usually, the filter membrane is arranged on the cover body of the sealing cap, the tissue culture bottle for tissue culture can carry out gas exchange by depending on the filter membrane type structure arranged on the bottle cap, but the used filter membranes have certain service life and are usually damaged due to friction, touch, aging and the like, so that the pollution phenomenon is caused, the loss of materials, the improvement of the pollution rate and the like are caused, and the quantity of top illumination entering the bottle is also influenced by the filter membrane structure.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a following technical scheme: a plant tissue culture bottle comprises a glass bottle and a bottle cap, wherein an air hole is formed between the central positions of the upper side wall and the lower side wall of the bottle cap, and a rubber sealing plug is fixedly connected to the bottom wall of the bottle cap and positioned on the outer side of the air hole in a concentric circle shape; pore inside wall slidable mounting has the tampon, the rubber sealing plug diapire is two connecting rods of symmetry fixedly connected with, connecting rod lateral wall slidable mounting has a plant constraint ring below, a spring clamp is established to the cover on the connecting rod, the connecting rod bottom is provided with the culture tank. When the plant constraint ring is fixed to needs through the spring clamp that sets up, only need loosen spring clamp's ears hard, make the inner circle grow, spring clamp just can press from both sides tight connecting rod, and the first side that slides from top to bottom needs presses spring clamp's ears hard, and the inner circle diminishes, can press from both sides tight connecting rod, realizes that the plant constraint ring is fixed on the connecting rod, and is very convenient. The bottle cap can be screwed to realize that the plants in the culture tank connected by the connecting rod can be taken out together, namely, the plants in the plant tissue culture bottle can be taken out conveniently. Plant constraint ring is liftable formula structural design, when cultivateing to the plant of co-altitude, the altitude mixture control of adaptability can be carried out to the height of its plant constraint ring for the plant of constraint cultivation prevents that it from empting.

Preferably, a through groove is formed between the center positions of the upper side wall and the lower side wall of the tampon, and a filter membrane is fixedly mounted on the inner side wall of the opening above the through groove. When the filter membrane is replaced, the cotton plug can be replaced by drawing out the cotton plug from the air hole, and the convenience are good.

Preferably, the outer side wall of the rubber sealing plug is connected with the inner side wall of the glass bottle in a sliding mode.

Further, two connecting rod diapire fixedly connected with connecting block, two connecting block diapire fixedly connected with culture tank.

Furthermore, a plurality of through holes are formed in the side wall and the bottom of the culture tank. Can be provided with appropriate amount culture solution in glass bottle bottom to wait to cultivate plant tissue culture in culture tank inside, the culture solution of glass bottle bottom can flow into the culture tank through the through-hole in, wait to cultivate that plant tissue can absorb culture solution vegetative growth, the root accessible through-hole that waits to cultivate plant tissue growth simultaneously grows to the outside, better absorption culture solution nutrition, and can prevent the unordered winding of plant root system in the plant tissue culture bottle, do benefit to simultaneously and take out the plant in the plant tissue culture bottle.

Furthermore, the outer diameter and the inner diameter of the plant binding ring are the same as those of the rubber sealing plug, and the opening of the plant binding ring and the opening of the rubber sealing plug are arranged in an up-down overlapped manner. When the whole culture structure is taken out from the opening above the culture tank, the convenience is better.

The utility model has the advantages as follows:

1. the utility model discloses a plant constraint ring through being provided with the lift design, when cultivateing to the plant of co-altitude, the altitude that its plant constraint ring can carry out the altitude mixture control of adaptability, can be provided with appropriate amount culture solution in glass bottle bottom, and wait to cultivate plant tissue culture in culture tank inside, the culture solution of glass bottle bottom can flow in the culture tank through the through-hole, it can absorb culture solution vegetative growth to wait to cultivate plant tissue, the root accessible through-hole that waits to cultivate plant tissue growth simultaneously grows to outside, better absorption culture solution is nutritious, and can prevent the unordered winding of plant root system in the plant tissue culture bottle, do benefit to simultaneously and take out the plant in the plant tissue culture bottle.

2. The utility model discloses a be provided with the gas pocket in plant tissue culture bottle lid roof to be provided with independent cotton plug structure in gas pocket top opening part, be provided with the filter membrane in the cotton plug structure, carry out the filter membrane and change the during operation, its convenient convenience is good.

Drawings

FIG. 1 is an overall perspective view of the present invention;

FIG. 2 is a perspective view of the culture structure of the present invention;

FIG. 3 is a perspective view of the other side of the cultivation structure of the present invention.

In the figure: 1. a glass bottle; 3. a bottle cap; 4. air holes; 5. a tampon; 6. a rubber sealing plug; 7. a connecting rod; 8. connecting blocks; 10. a culture tank; 11. a through hole; 12. a plant restraint loop; 13. a spring clip 13.

Detailed Description

The following description of the preferred embodiments of the present invention will be provided with reference to the accompanying drawings, so that the advantages and features of the present invention can be easily understood by those skilled in the art, and the scope of the present invention can be clearly and clearly defined.

Referring to fig. 1 and 3, a plant tissue culture bottle comprises a glass bottle 1 and a bottle cap 3, wherein an air hole 4 is formed between the central positions of the upper side wall and the lower side wall of the bottle cap 3, a rubber sealing plug 6 is fixedly connected to the bottom wall of the bottle cap 3 and positioned on the outer side of the air hole 4 in a concentric circle shape, a cotton plug 5 is slidably mounted on the inner side wall of the air hole 4, connecting rods 7 are fixedly connected to the bottom wall of the rubber sealing plug 6 in a symmetrical manner, and a plant binding ring 12 is slidably mounted on the outer side walls of the two connecting rods 7. Plant constraint ring 12 is liftable formula structural design, and when the plant to co-altitude is cultivateed, the altitude mixture control of adaptability can be carried out to the height of its plant constraint ring 12 for the plant of constraint cultivation prevents that it from empting. The bottom of the two connecting rods 7 is provided with a culture tank 10, and a spring hoop 13 is sleeved on the connecting rods 7 below the plant binding ring 12. Through the spring clamp 13 that sets up, press the ears of spring clamp 13 hard, adjust spring clamp 13 to the suitable position in fixed plant constraint ring 12 below, loosen the ears of spring clamp 13, make the inner circle diminish, spring clamp 13 just can press from both sides tight connecting rod, realizes that the plant constraint ring 12 is fixed on the connecting rod and freely slides from top to bottom, and is very convenient. The bottle cap 3 can be screwed to realize that the plants in the culture tank 10 connected by the connecting rod 7 can be taken out together, i.e. the plants in the plant tissue culture bottle can be taken out conveniently.

As shown in figures 1 and 2, the outer diameter and the inner diameter of the plant-bound ring 12 are the same as those of the rubber sealing plug 6, the opening of the plant-bound ring 12 and the opening of the rubber sealing plug 6 are arranged in an up-and-down overlapped manner, and the whole culture structure is convenient to take out from the opening of the glass bottle 1.

As shown in figure 1, a through groove is formed between the central positions of the upper side wall and the lower side wall of the tampon 5, a filter membrane is fixedly mounted on the inner side wall of the opening above the through groove, and when the filter membrane is replaced, the tampon 5 can be drawn out through the air hole 4 to be replaced, so that the convenience and the convenience are good.

As shown in fig. 1-3, the bottom walls of the two connecting rods 7 are fixedly connected with connecting blocks 8, the bottom walls of the two connecting blocks 8 are fixedly connected with culture tanks 10, and a plurality of through holes 11 are formed on the outer side walls and the bottoms of the peripheral sides of the culture tanks 10. Glass bottle bottom is equipped with appropriate amount culture solution or culture medium to wait to cultivate plant tissue culture in culture tank 10, the culture solution can flow into in culture tank 10 via through-hole 11, it can absorb the culture solution vegetative growth to wait to cultivate plant tissue, the root accessible through-hole 11 of waiting to cultivate plant tissue growth simultaneously grows to the outside, absorb the culture solution nutrition better, and can prevent the unordered winding of plant root system in the plant tissue culture bottle, do benefit to simultaneously and take out the plant in the plant tissue culture bottle.

The utility model discloses a theory of operation: when using, can place appropriate amount culture solution or culture medium in glass bottle 1 bottom, and in culture tank 10 inside waiting to cultivate plant tissue culture, culture solution or culture medium can flow into culture tank 10 via through-hole 11 in, wait to cultivate vegetative growth in the plant tissue absorbable culture solution or the culture medium, the root accessible through-hole 11 of waiting to cultivate plant tissue growth simultaneously grows to the outside, the culture solution or culture medium nutrition in the better absorption glass bottle 1, and can prevent the unordered winding of plant root system in the plant tissue culture bottle, do benefit to the plant taking out in the plant tissue culture bottle simultaneously. Plant constraint ring 12 is liftable formula structural design, and when the plant to co-altitude is cultivateed, the altitude of its plant constraint ring 12 can carry out the altitude mixture control of adaptability for the plant of constraint cultivation prevents that it from empting. When the filter membrane is replaced, the tampon 5 can be drawn out through the air hole 4 to replace the filter membrane, and the convenience are good. In addition, the bottle cap 3 can be screwed to draw out the opening of the glass bottle 1, and at the moment, plants in the culture tank 10 connected with the bottom of the bottle cap 3 through the connecting rod 7 can be taken out together, namely, the plants in the plant tissue culture bottle are taken out conveniently, and the damage to the plants is small.

Claims

1. The utility model provides a plant tissue culture bottle, includes glass bottle (1) and bottle lid (3), its characterized in that: an air hole (4) is formed between the central positions of the upper side wall and the lower side wall of the bottle cap (3), and a rubber sealing plug (6) is fixedly connected to the bottom wall of the bottle cap (3) and is positioned on the outer side of the air hole (4) in a concentric circle shape; pore (4) inside wall slidable mounting has tampon (5), rubber sealing plug (6) diapire is two connecting rods (7) of symmetry fixedly connected with, connecting rod (7) lateral wall slidable mounting has a plant constraint ring (12) below, a spring clamp (13) are established to the cover on connecting rod (7), connecting rod (7) bottom is provided with cultivates groove (10).

2. The plant tissue culture flask according to claim 1, wherein: a through groove is formed between the center positions of the upper side wall and the lower side wall of the tampon (5), and a filter membrane is fixedly mounted on the inner side wall of the opening above the through groove.

3. The plant tissue culture flask according to claim 1, wherein: the outer side wall of the rubber sealing plug (6) is connected with the inner side wall of the glass bottle (1) in a sliding manner.

4. The plant tissue culture flask according to claim 1, wherein: two connecting rod (7) diapire fixedly connected with connecting block (8), two connecting block (8) bottom fixedly connected with culture tank (10).

5. The plant tissue culture flask according to claim 4, wherein: the side wall and the bottom of the culture tank (10) are provided with a plurality of through holes (11).

6. The plant tissue culture flask according to claim 1, wherein: the outer diameter and the inner diameter of the plant binding ring (12) are the same as those of the rubber sealing plug (6), and the opening of the plant binding ring (12) and the opening of the rubber sealing plug (6) are arranged in an up-and-down overlapped manner.