CN211379056U - Tremella aurantialba koji culture device - Google Patents

Tremella aurantialba koji culture device Download PDF

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CN211379056U
CN211379056U CN201922383157.8U CN201922383157U CN211379056U CN 211379056 U CN211379056 U CN 211379056U CN 201922383157 U CN201922383157 U CN 201922383157U CN 211379056 U CN211379056 U CN 211379056U
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culture
air
tube
air duct
threaded connection
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王厚鹏
王国庆
张秀停
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Shandong Yuanyang Agriculture Development Co ltd
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Shandong Yuanyang Agriculture Development Co ltd
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Abstract

The utility model discloses a golden fungus koji culture device, belonging to the technical field of mushroom production equipment, comprising a culture frame and a bag sleeving structure arranged on the culture frame; the method is characterized in that: the culture shelf is provided with culture chambers with a preset number of layers, and is also provided with a sterile ventilation pipeline and an air return pipeline; the cover bag structure includes that the cover is established interior sleeve pipe and outer tube together through threaded connection, and the axle center of tube cap is provided with the air duct through threaded connection, the beneficial effects of the utility model are that: the inner sleeve and the outer sleeve which are sleeved together are connected in a threaded manner, so that the sealing property of the device is improved, and the problem that bacteria are easily infected in the early stage of inoculation is solved; the air duct of culture medium combines the aseptic air duct who has the air regulating valve to carry out near contact regulation and control to culture medium's oxygen, humidity and temperature effectively, has avoided current cover bag and external environment to keep apart, leads to leading to the aeration effect relatively poor, the lower problem of golden fungus yield.

Description

Tremella aurantialba koji culture device
The technical field is as follows:
the utility model belongs to the technical field of fungus mushroom production facility, more specifically relates to a golden fungus seed leaven culture apparatus.
Background art:
the Tremella aurantialba refers to fungi of phylum Eumycota, and is also called Tremella flava, Tremella aurantialba, and Tremella cerebri. The sporocarp is scattered or gathered, and the surface is smooth; gradually grow up to the early stage of maturity, the base of the ear is wedge-shaped, the upper part is uneven, distorted and thickened, the shape is like brain or irregular split petal, and the internal tissue is full.
The cultivation process of the tremella aurantialba is similar to that of common fungi, and comprises strain preparation and strain preparation, wherein the strain preparation comprises strain bag planting, strain stick planting and strain bottle planting; the culture process of the tremella aurantialba is different from that of common fungi, and the tremella aurantialba hyphae comprises white roughage leatherette hyphae and light yellow hyphae during strain preparation, so when the tremella aurantialba hyphae are used for inoculating original strains, white and light yellow hyphae are required to be used for inoculation to be successful;
under the normal condition of planting the fungus bags, the seed blocks germinate and plant for 4-5 days, the hard leather hyphae overgrow in the bags after 20-25 days, the inoculated blocks increase, and when the temperature is 20-25 ℃ and the relative air humidity is 85-90%, the golden fungus hyphae grow rapidly and enter the fruiting period.
At this time, the temperature is reduced to 15 ℃ to inhibit the growth of the hypha of the Rough crust leather and promote the growth of the primordium of the golden fungus sporocarp. If no temperature reduction measures are taken, the phanerochaete hirsutum can continuously grow vigorously and continuously expand to form a disc-shaped wrapping golden fungus sporocarp, so that the golden fungus sporocarp is prevented from developing and even dying. When the tremella aurantialba fruiting body grows to 4 cm multiplied by 4 cm, the tampon should be pulled out to increase oxygen; when the length reaches 6-8 cm, the neck of the bag is taken off, and the bag is straightened to accelerate the growth under the conditions of calm wind, oxygen and proper temperature. As the fruit body grows, the bag mouth should be turned down. Exposing the fruiting body to the air, prolonging the illumination time, and collecting the fruiting body which is orange yellow or orange red. Each bag can produce 80-100 g of fresh fungus, and the high yield can reach more than 180 g. After harvesting, managing according to the method, straightening the bag mouth to restore the original shape, culturing for 10-15 days, growing a second batch of tremella aurantialba on the base of the harvested sporocarp, wherein each bag can produce 30-50 g of fresh tremella aurantialba, the fresh tremella aurantialba accounts for about 30% of the total yield, and the total biological efficiency can reach 90-100%. The whole production cycle from inoculation to harvest is 55-70 days.
The fungus bag is planted output is not high all the time, this is because the golden fungus requires highly to the temperature and humidity and the logical oxygen that the fungus bag was planted, and every growth phase requires all differently to temperature and humidity and logical oxygen, adopts general fungus bag to plant, because the fungus bag belongs to independent space for external environment, and the temperature and humidity and logical oxygen regulation and control degree of difficulty are great, increases the vent of fungus bag, increases the possibility of infecting fungus, need carry out aseptic shutoff to the fungus bag in earlier stage of growing, leads to temperature and humidity and logical oxygen extremely difficult regulation and control.
The utility model has the following contents:
in order to solve the problems and overcome the defects of the prior art, the utility model provides a golden fungus koji culture device;
the first technical problem to be solved is that: the tremella aurantialba has higher requirements on temperature, humidity and oxygen supply for fungus bag planting, and the requirements on temperature, humidity and oxygen supply in each growth stage are different;
the second technical problem to be solved is that: the fungus bag belongs to independent space for the external environment, and the temperature and humidity and the regulation and control degree of difficulty of oxygen are great, in order to reduce the possibility of infecting fungus, need carry out aseptic shutoff to the fungus bag in earlier stage of growing, lead to temperature and humidity and lead to oxygen to regulate and control extremely difficultly
The utility model provides a concrete technical scheme of above-mentioned technical problem does: the golden fungus koji culture device comprises a culture frame and a bag sleeving structure arranged on the culture frame; the method is characterized in that: the culture shelf is provided with a preset number of layers of culture chambers, the culture chambers are arranged in a structural form with two open ends, the bottom of each culture chamber is provided with a supporting plate with an inclined surface structure, the upper end surface of each supporting plate is provided with a preset number of fixing seats, and each fixing seat is provided with a fixing notch connected with a fixed bagging structure in an inserting manner; the culture rack is also provided with a sterile ventilation pipeline and an air return pipeline;
the bagging structure comprises an inner sleeve and an outer sleeve which are sleeved together through threaded connection, the inner sleeve comprises a tube cover, the upper end face of the tube cover is provided with a tube body for loading culture base materials in a reverse buckling mode, the axis of the tube cover is provided with air guide tubes through threaded connection, the air guide tubes are provided with air holes, and the outer wall of the tube cover is provided with through holes for fruiting; a boss which is connected with the fixed notch in an inserting manner is arranged on the lower end surface of the tube cover outside the air guide tube and extends outwards;
the utility model discloses a pipe cover, including outer tube back-off setting, the outer lateral surface of outer tube is fixed with the lateral surface of tube cap including the outer tube back-off setting to through threaded connection's mode, the gas outlet has been seted up to the up end of outer tube, still is provided with the observation window that is used for observing on the pipe wall of outer tube.
Furthermore, sterile sealing caps are arranged on the outer sides of the air duct and the air outlet.
Furthermore, the aseptic vent pipe is provided with a preset number of air regulating valves, and the air regulating valves are connected with one end of the air guide pipe through vent pipes.
Further, the return air pipeline is provided with interfaces used for presetting the number, and the interfaces are connected with the air outlet through the vent pipe.
Furthermore, the fixing seat and the horizontal direction form a preset angle, and the fixing seat with the fixing notch forms an inclined plane structure.
The utility model has the advantages that:
the device is provided with the inner sleeve and the outer sleeve which are sleeved together through threaded connection, so that the sealing performance of the device is improved, and the problem that bacteria are easily infected in the early stage of inoculation is solved;
meanwhile, the air guide tube arranged on the culture base material is utilized, and the sterile air duct with the air regulating valve is combined to effectively regulate and control oxygen, humidity and temperature of the culture base material in a near contact mode, so that the culture medium is suitable for different requirements of different culture stages of the tremella aurantialba on oxygen, humidity and temperature, the problems that the oxygen passing effect is poor and the yield of the tremella aurantialba is low due to the fact that the existing sleeve bag is isolated from the external environment are avoided.
Description of the drawings:
FIG. 1 is a schematic structural diagram of an embodiment of the present invention at an early stage of cultivation;
FIG. 2 is a schematic structural view of the ear emergence period of the embodiment of the present invention;
FIG. 3 is a structural diagram of the bag of the present invention;
FIG. 4 is a schematic view of the inner sleeve structure of the present invention; in the drawings:
1. a boss; 2. a tube cover; 3. a pipe body; 4. a through hole; 5. an inner sleeve; 6. an air duct; 7. a sterile sealing cap; 8. an air outlet; 9. an outer sleeve; 10. an observation window; 11. a culture shelf; 12. a support plate; 13. a sterile vent line; 14. a return air duct; 15. a fixed seat; 16. a fixed notch; 17. and a gas regulating valve.
The specific implementation mode is as follows:
to further clarify the objects, aspects and advantages of the present invention, a more complete description of the invention will now be rendered by reference to the appended drawings, which are illustrated in fig. 1-4. In describing the present invention, it should be understood that the terms "center", "upper", "lower", "left", "right", "rear", "lower left", "upper right", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the scope of the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The utility model discloses a concrete implementation mode: the golden fungus koji culture device comprises a culture rack 11 and a bag sleeving structure arranged on the culture rack 11; the method is characterized in that: the culture shelf 11 is provided with a preset number of layers of culture chambers, the culture chambers are arranged in a structural form with two open ends, the bottom of each culture chamber is provided with a support plate 12 with an inclined surface structure, the upper end surface of each support plate 12 is provided with a preset number of fixed seats 15, and the fixed seats 15 are provided with fixed notches 16 connected with fixed bag sleeving structures in an inserting manner; the fixed seat 15 is arranged at a preset angle with the horizontal direction, and the fixed seat 15 with the fixed notch 16 forms an inclined plane structure;
the culture shelf 11 is also provided with a sterile ventilation pipeline 13 and an air return pipeline 14; the sterile vent pipeline 13 is provided with a preset number of air regulating valves 17, and the air regulating valves 17 are connected with one end of the air guide pipe 6 through vent pipes; the air return pipeline 14 is provided with interfaces for presetting number, and the interfaces are connected with the air outlet 8 through an air pipe;
the bagging structure comprises an inner sleeve 5 and an outer sleeve 9 which are sleeved together through threaded connection, the inner sleeve 5 comprises a tube cover 2, a tube body 3 used for loading culture base materials is arranged on the upper end face of the tube cover 2 in a reverse buckling mode, the axis of the tube cover 2 is provided with air guide tubes 6 through threaded connection, the air guide tubes 6 are all provided with air holes, and the outer wall of the tube cover 2 is provided with through holes 4 used for fruiting; the lower end surface of the tube cover 2 at the outer side of the air duct 6 extends outwards and is provided with a boss 1 which is connected with the fixed notch 16 in an inserting way;
the outer sleeve 9 is arranged on the outer side of the inner sleeve 5 in an inverted manner and is fixed with the outer side surface of the tube cover 2 in a threaded connection manner, the upper end surface of the outer sleeve 9 is provided with an air outlet 8, and the tube wall of the outer sleeve 9 is also provided with an observation window 10 for observation; sterile sealing caps 7 are arranged on the outer sides of the air duct 6 and the air outlet 8.
It is noted that the utility model relates to a golden fungus seed koji culture device, when in specific work,
1. loading culture medium into the tube body 3, assembling the inner sleeve 5 and the outer sleeve 9 together, plugging an aseptic sealing cap 7 containing an aseptic filter membrane, putting the aseptic sealing cap into an autoclave, sterilizing at high temperature, cooling, opening the outer sleeve 9 in an aseptic operation table, unscrewing an air duct 6 of the inner sleeve 5, putting a mycelial cluster containing white roughhaired crust leather hyphae and golden fungus hyphae of light yellow hyphae into the aseptic operation table, connecting the outer sleeve 9, disinfecting the connection part by using alcohol, and plugging by using the aseptic sealing cap 7;
2. the sterile tube is connected with the sterile sealing cap 7, the boss 1 for fixing the bagging structure is inserted in the fixing groove opening 16, the bagging structure cannot fall off due to interference fit at the moment, the sterile tube is respectively connected with the sterile ventilation pipeline 13 and the air return pipeline 14, the air regulating valve 17 is regulated, the growth requirement of a first gradient is met by regulating the humidity of sterile air, the sterile air enters the air guide tube 6 and penetrates through materials, enters the outer sleeve 9 through the through hole 4 and is discharged into the air return pipeline 14 through the air outlet 8, and the air inflow can be controlled by regulating the size of the air regulating valve 17;
under the normal condition of planting in a fungus bag, seed blocks germinate and plant for 4-5 days, the hard leather hyphae overgrow in the bag after 20-25 days, the inoculated blocks increase, and when the temperature is 20-25 ℃ and the relative air humidity is 85-90%, the golden fungus hyphae grow rapidly and enter the fruiting period;
3. and (3) introducing air with large moisture content during the fruiting period, and cooling to 15 ℃ to inhibit the growth of the scleroderma harorum hyphae and promote the growth of the primordium of the tremella aurantialba sporocarp. The phanerochaete hirsutum can continuously grow vigorously and continuously expand to form a disc-shaped wrapping tremella aurantialba sporocarp, so that the tremella aurantialba sporocarp is prevented from developing and even dying; at the moment, the inner sleeve 5 and the outer sleeve 9 are in a closed environment, and the air is introduced to regulate the humidity, so that the accuracy is high, the whole system is separated from the external environment, and bacteria are not easily infected;
3. when the golden fungus solid is seen to grow to 4 cm multiplied by 4 cm through the observation window, the outer sleeve 9 is removed, and the air inlet quantity is increased by adjusting the air regulating valve 17; the high oxygen demand in the period is ensured, and simultaneously the water content of the sterile gas can be properly improved, so that the water loss of the inner sleeve 5 caused by the air flow is prevented;
4. exposing the fruiting body with air, and collecting when the fruiting body grows up and the illumination time is prolonged and the fruiting body is orange yellow or orange red.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (5)

1. A golden fungus koji culture device comprises a culture rack (11) and a bag structure arranged on the culture rack (11); the method is characterized in that: the culture rack (11) is provided with a preset number of layers of culture chambers, the culture chambers are arranged in a structural form with two open ends, the bottom of each culture chamber is provided with a supporting plate (12) with an inclined surface structure, the upper end surface of each supporting plate (12) is provided with a preset number of fixing seats (15), and the fixing seats (15) are provided with fixing notches (16) connected with a fixed bagging structure in an inserting manner; the culture rack (11) is also provided with a sterile ventilation pipeline (13) and an air return pipeline (14);
the bagging structure comprises an inner sleeve (5) and an outer sleeve (9) which are sleeved together through threaded connection, the inner sleeve (5) comprises a tube cover (2), the upper end face of the tube cover (2) is provided with a tube body (3) used for loading a culture base material in an inverted buckling mode, the axis of the tube cover (2) is provided with air guide tubes (6) through threaded connection, the air guide tubes (6) are all provided with air holes, and the outer wall of the tube cover (2) is provided with through holes (4) used for fruiting; the lower end surface of the tube cover (2) at the outer side of the air duct (6) extends outwards and is provided with a boss (1) which is connected with the fixed notch (16) in an inserting way;
the utility model discloses a pipe cover, including outer tube (9) back-off setting in the outside of sleeve pipe (5) to it is fixed with the lateral surface of tube cap (2) through threaded connection's mode, gas outlet (8) have been seted up to the up end of outer tube (9), still are provided with observation window (10) that are used for the observation on the pipe wall of outer tube (9).
2. A golden fungus koji culture apparatus according to claim 1, wherein the outer sides of the air duct (6) and the air outlet (8) are provided with sterile sealing caps (7).
3. A koji culture apparatus according to claim 1, wherein the sterile vent line (13) is provided with a predetermined number of air regulating valves (17), the air regulating valves (17) being connected to one end of the air duct (6) through vent pipes.
4. A koji culture apparatus according to claim 1, wherein the return air duct (14) is provided with a predetermined number of ports which are connected to the air outlet (8) via a vent pipe.
5. A mold for a mother culture of auris starter according to claim 1, characterized in that the holder (15) is arranged at a predetermined angle to the horizontal, and the holder (15) with the holding notch (16) is formed as a slope structure.
CN201922383157.8U 2019-12-26 2019-12-26 Tremella aurantialba koji culture device Active CN211379056U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413533A (en) * 2022-10-09 2022-12-02 福建万辰生物科技股份有限公司 Culture device and culture method for hypsizigus marmoreus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413533A (en) * 2022-10-09 2022-12-02 福建万辰生物科技股份有限公司 Culture device and culture method for hypsizigus marmoreus

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GR01 Patent grant
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of utility model: A golden ear koji culture device

Effective date of registration: 20220609

Granted publication date: 20200901

Pledgee: Agricultural Bank of China Limited Liangshan County sub branch

Pledgor: SHANDONG YUANYANG AGRICULTURE DEVELOPMENT Co.,Ltd.

Registration number: Y2022980007277

PE01 Entry into force of the registration of the contract for pledge of patent right