CN109042075A - Fang County's local ecosystem edible fungus species raising technology - Google Patents

Fang County's local ecosystem edible fungus species raising technology Download PDF

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Publication number
CN109042075A
CN109042075A CN201811073253.6A CN201811073253A CN109042075A CN 109042075 A CN109042075 A CN 109042075A CN 201811073253 A CN201811073253 A CN 201811073253A CN 109042075 A CN109042075 A CN 109042075A
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bacteria stick
parent species
original seed
cultivar
species
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段家忠
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/40Cultivation of spawn
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Ecosystem edible fungus species raising technology in Fang County local of the present invention belongs to edible mushroom technical field, using the mycelium of the wild black fungus strain of ecosystem of Fang County's local intermountain acquisition as parent species, miscellaneous bacteria and impurity are subjected to separating-purifying by modern advanced Scientific Research Equipments, black fungus strain parent species after purification are put into culturing room and are cultivated, the parent species for cultivating the black fungus strain finished carry out batch breeding into original seed by sterilized bacteria stick, bacteria stick can be planted with bag by, which finishing to Initial strain breeding culture, is inoculated with, it cultivates, grow the cultivar of black fungus strain, the edible fungus species maintain the quality and mouthfeel of Fang County's local black fungus to greatest extent.

Description

Fang County's local ecosystem edible fungus species raising technology
Technical field
Ecosystem edible fungus species raising technology in Fang County local of the present invention belongs to edible mushroom technical field, in particular to Fang County Acquisition, purification, culture, the breeding of local ecosystem edible fungus species.
Background technique
Fang County, ancient times claim room state, positioned at Northwest of Hubei Province area, the adjacent virgin forest Shennongjia in south, and the adjacent Holy Land of the Taoism in north Wudang Mountain, Long-term 12 DEG C -19 DEG C of temperature on average, four seasons abundant rainfall, the long-term fog of intermountain curl up, and are known as Chinese " natural Drug Storage ".Fang County Local " king of mushroom " black fungus the most famous, according to it is a it is big, color is bright, meat is thick, crisp, nutritive value is high, enjoy great prestige China and foreign countries. Black fungus has more than one thousand years in Fang County's plantation history, and history records record Tang, and just just existing peasant household largely plants.But as the modern times pass through The rapid development of Ji, part farmer in order to be the advantages of devoting exclusive attention to output and economic benefit, selected new edible fungus species, the strain The black fungus speed of growth is fast, yield is slightly above the black fungus of local edible fungus species growth, the disadvantage is that the black fungus meat grown Thin, color is whitened, mouthfeel skin is lower than the black fungus of local growth without crisp, nutritional ingredient.
Summary of the invention
Ecosystem edible fungus species raising technology in Fang County local of the present invention, the ecosystem using the acquisition of Fang County's local intermountain are wild The mycelium of raw black fungus strain as parent species, by modern advanced Scientific Research Equipments by miscellaneous bacteria and impurity carry out separating-purifying, Black fungus strain parent species after purification are put into culturing room and are cultivated, cultivated the parent species of the black fungus strain finished by efficiently going out The bacteria stick of bacterium carries out batch breeding into original seed, and bacteria stick can be planted with bag by, which finishing to Initial strain breeding culture, is inoculated with, cultivated, grown The cultivar of black fungus strain, the edible fungus species maintain the quality and mouthfeel of Fang County's local black fungus to greatest extent.
Fang County's local ecosystem edible fungus species raising technology, it is characterized in that steps are as follows:
A acquires parent species, the lunar calendar 3-4 month takes the tweezers sterilized, cutter, test tube, tampon, the local separate resident's of Fang County Wild black fungus is found on mountain, and the mycelium above the birch robur of black fungus parasitism is examined after finding, it will be with tweezers The bark of white hypha body peels off, then entirely cuts the preferable mycelium of growing way above tree trunk with cutter, be packed into test tube, stopper Tampon, different places, need to acquire 30 pipes or more in total it is spare;
The purification of b parent species, by the purification case of 1m3 using fastening chamber door after being wiped repeatedly inside and outside 85% alcohol, then can amplify 500 times of microscope, tweezers, cutter, sheet glass, feeding solution, suction pipe, spare test tube are put into after being wiped repeatedly using alcohol and are mentioned Pure case, is put into purification case after the tubing surfaces alcohol foot equipped with parent species is wiped across, technical staff is by head from purification case Nose sleeve protrude into purification case, both hands from hand casing protrude into purification case, sheet glass is placed on microscopical by technical staff Above bracket, open equipped with parent species test tube tampon, parent species are pressed from both sides out to be placed on above sheet glass with tweezers and are observed, technology people Member rooted out by the fructification of micro- sem observation mycelia, by the fructification of aging and impurity with cutter, the preferable purity of retentive activity Higher mycelium, the nutrient solution (mixture of sucrose, murphy juice, agar, water by disinfection) that 5ml is sucked with suction pipe are put into standby The spare test tube equipped with nutrient solution is put into test tube, by well-graded parent species, stopper tampon after be placed on side, successively by test tube In parent species sorting finish;
C Initial strain breeding, will equipped with purification parent species test tube be put into incubator, temperature setting be 15 DEG C -20 DEG C culture after a week, see It examines the growing state of parent species, eliminate the parent species for infecting miscellaneous bacteria, while cultivating parent species, should prepare to breed original seed as early as possible Raw material, the raw material of original seed are bulky grain sawdust 70%, wheat bran 28%, gypsum 1%, sugared 1%, appropriate amount of water, first by the former material of original seed Original seed bacteria stick is put into high-pressure sterilizing pot after being packed, packed using sack filling machine after being sufficiently stirred plus is fitted by material It is heated after amount water, sealed pot cover, the cooling that takes the dish out of the pot after 6h, sterilizing should be kept when autoclave air pressure inside reaches 2KPa To room temperature, sterilized original seed bacteria stick and parent species are transferred in the transfer room through strict sterilization, are then placed in inoculating hood, by technology Parent species are sequentially ingressed among the bacteria stick of original seed by personnel according to operating instruction, be inoculated with the original seed bacteria stick finished be transferred into culturing room into Row culture, culturing room temperature be maintained between 15 DEG C -20 DEG C, air humidity between 50%-60%, indoor needs often divulge information Ventilation;
The breeding of d cultivar, original seed should often be stirred when culturing room is cultivated bacteria stick observed, find mycelia jaundice or The bacteria stick of nigrescence is eliminated, can be connect after mycelia is covered with 80% position of bacteria stick when being the bacteria stick emergency for having infected miscellaneous bacteria Kind, prepare the raw material of cultivar when cultivating the later period emergency of original seed, the raw material of cultivar are sawdust 80%, wheat bran 18%, stone Cream 1%, sugared 1%, appropriate amount of water, after being packed, packed using sack filling machine after the raw material of cultivar being sufficiently stirred It is kept after being put into normal-pressure sterilization room by cultivar bacteria stick, the temperature inside sterilization chamber is risen to 100 DEG C using boiler generation steam It carries out being cooled to room temperature the transfer room that bacteria stick moves to strict sterilization after high-temperature sterilization, sterilizing for 24 hours, by original seed and The cultivar bacteria stick of sterilizing be put into inoculating hood, by technical staff by original seed according to operating instruction be sequentially ingressed into cultivar bacteria stick it In, be inoculated with the cultivar bacteria stick finished be transferred into culturing room is cultivated, the temperature of culturing room is maintained between 15 DEG C -20 DEG C, Air humidity between 50%-60%, the frequent ventilation of indoor needs, can be with life after mycelia is covered with 80% position of bacteria stick The bacteria stick of long black fungus is inoculated with.
The utility model has the advantages that Fang County local part farmer has selected new edible fungi to devote exclusive attention to output and economic benefit The advantages of kind, the strain is the black fungus for the edible fungus species growth that the black fungus speed of growth is fast, yield is slightly above local, disadvantage Be growth black fungus meat it is thin, color is whitened, mouthfeel skin is lower than the black fungus of local growth without crisp, nutritional ingredient.Room County's ecosystem edible fungus species raising technology utilizes the bacterium of the wild black fungus strain of ecosystem of Fang County's local intermountain acquisition Miscellaneous bacteria and impurity are carried out the black fungus bacterial after separating-purifying, purification as parent species, by modern advanced Scientific Research Equipments by filament Kind of parent species, which are put into culturing room, is cultivated, is cultivated the parent species of the black fungus strain finished and carried out in batches by sterilized bacteria stick Original seed is bred into, the cultivation that bacteria stick is inoculated with, cultivates, grows black fungus strain can be planted with bag by finishing to Initial strain breeding culture Kind, which maintains the quality and mouthfeel of Fang County's local black fungus to greatest extent.
Specific embodiment
Now in conjunction with the above-mentioned embodiment that the present invention will be described in detail.
Embodiment
Fang County's local ecosystem edible fungus species raising technology, it is characterized in that steps are as follows:
A acquires parent species, the lunar calendar 3-4 month takes the tweezers sterilized, cutter, test tube, tampon, the local separate resident's of Fang County Wild black fungus is found on mountain, and the mycelium above the birch robur of black fungus parasitism is examined after finding, it will be with tweezers The bark of white hypha body peels off, then entirely cuts the preferable mycelium of growing way above tree trunk with cutter, be packed into test tube, stopper Tampon, different places, need to acquire 30 pipes or more in total it is spare;
The purification of b parent species, by the purification case of 1m3 using fastening chamber door after being wiped repeatedly inside and outside 85% alcohol, then can amplify 500 times of microscope, tweezers, cutter, sheet glass, feeding solution, suction pipe, spare test tube are put into after being wiped repeatedly using alcohol and are mentioned Pure case, is put into purification case after the tubing surfaces alcohol foot equipped with parent species is wiped across, technical staff is by head from purification case Nose sleeve protrude into purification case, both hands from hand casing protrude into purification case, sheet glass is placed on microscopical by technical staff Above bracket, open equipped with parent species test tube tampon, parent species are pressed from both sides out to be placed on above sheet glass with tweezers and are observed, technology people Member rooted out by the fructification of micro- sem observation mycelia, by the fructification of aging and impurity with cutter, the preferable purity of retentive activity Higher mycelium, the nutrient solution (mixture of sucrose, murphy juice, agar, water by disinfection) that 5ml is sucked with suction pipe are put into standby The spare test tube equipped with nutrient solution is put into test tube, by well-graded parent species, stopper tampon after be placed on side, successively by test tube In parent species sorting finish;
C Initial strain breeding, will equipped with purification parent species test tube be put into incubator, temperature setting be 15 DEG C -20 DEG C culture after a week, see It examines the growing state of parent species, eliminate the parent species for infecting miscellaneous bacteria, while cultivating parent species, should prepare to breed original seed as early as possible Raw material, the raw material of original seed are bulky grain sawdust 70%, wheat bran 28%, gypsum 1%, sugared 1%, appropriate amount of water, first by the former material of original seed Original seed bacteria stick is put into high-pressure sterilizing pot after being packed, packed using sack filling machine after being sufficiently stirred plus is fitted by material It is heated after amount water, sealed pot cover, the cooling that takes the dish out of the pot after 6h, sterilizing should be kept when autoclave air pressure inside reaches 2KPa To room temperature, sterilized original seed bacteria stick and parent species are transferred in the transfer room through strict sterilization, are then placed in inoculating hood, by technology Parent species are sequentially ingressed among the bacteria stick of original seed by personnel according to operating instruction, be inoculated with the original seed bacteria stick finished be transferred into culturing room into Row culture, culturing room temperature be maintained between 15 DEG C -20 DEG C, air humidity between 50%-60%, indoor needs often divulge information Ventilation;
The breeding of d cultivar, original seed should often be stirred when culturing room is cultivated bacteria stick observed, find mycelia jaundice or The bacteria stick of nigrescence is eliminated, can be connect after mycelia is covered with 80% position of bacteria stick when being the bacteria stick emergency for having infected miscellaneous bacteria Kind, prepare the raw material of cultivar when cultivating the later period emergency of original seed, the raw material of cultivar are sawdust 80%, wheat bran 18%, stone Cream 1%, sugared 1%, appropriate amount of water, after being packed, packed using sack filling machine after the raw material of cultivar being sufficiently stirred It is kept after being put into normal-pressure sterilization room by cultivar bacteria stick, the temperature inside sterilization chamber is risen to 100 DEG C using boiler generation steam It carries out being cooled to room temperature the transfer room that bacteria stick moves to strict sterilization after high-temperature sterilization, sterilizing for 24 hours, by original seed and The cultivar bacteria stick of sterilizing be put into inoculating hood, by technical staff by original seed according to operating instruction be sequentially ingressed into cultivar bacteria stick it In, be inoculated with the cultivar bacteria stick finished be transferred into culturing room is cultivated, the temperature of culturing room is maintained between 15 DEG C -20 DEG C, Air humidity between 50%-60%, the frequent ventilation of indoor needs, can be with life after mycelia is covered with 80% position of bacteria stick The bacteria stick of long black fungus is inoculated with.
According to above-mentioned Fang County local ecosystem edible fungus species raising technology, the ecosystem acquired using Fang County's local intermountain The mycelium of wild black fungus strain as parent species, by parent species purifies breeding original seed, original seed, and breeding cultivation kind, cultivar be again again The black fungus of inoculation output Fang County local ecosystem is carried out with the bacteria stick of growth black fungus, which protects to greatest extent The quality and mouthfeel of Chi Liao Fang County local black fungus.
The foregoing is merely routines of the invention to disclose, and is not intended to limit the present invention in any form;It is all to be familiar with sheet The technical staff of industry within the scope of without departing from technical solution of the present invention, implement techniques described above is made it is any equivalent Adjustment, modification and differentiation of variation etc., in the protection scope for still falling within technical solution of the present invention.

Claims (1)

1. Fang County's local ecosystem edible fungus species mating system, it is characterized in that steps are as follows:
A. parent species are acquired, the lunar calendar 3-4 month take the tweezers sterilized, cutter, test tube, tampon, the local separate resident's of Fang County Wild black fungus is found on mountain, and the mycelium above the birch robur of black fungus parasitism is examined after finding, it will be with tweezers The bark of white hypha body peels off, then entirely cuts the preferable mycelium of growing way above tree trunk with cutter, be packed into test tube, stopper Tampon, different places, need to acquire 30 pipes or more in total it is spare;
B. parent species purify, and by the purification case of 1m3 using fastening chamber door after being wiped repeatedly inside and outside 85% alcohol, then can put Big 500 times of microscope, tweezers, cutter, sheet glass, feeding solution, suction pipe, spare test tube are put into after being wiped repeatedly using alcohol Case is purified, is put into purification case after the tubing surfaces alcohol foot equipped with parent species is wiped across, technical staff is by head from purification The nose sleeve of case protrudes into purification case, both hands from hand casing protrude into purification case, and sheet glass is placed on microscope by technical staff Bracket above, open equipped with parent species test tube tampon, parent species are pressed from both sides out to be placed on above sheet glass with tweezers and are observed, technology Personnel are rooted out by the fructification of micro- sem observation mycelia, by the fructification of aging and impurity with cutter, retentive activity is preferably pure It spends higher mycelium, be put into the nutrient solution (mixture of sucrose, murphy juice, agar, water by disinfection) of suction pipe sucking 5ml Spare test tube, well-graded parent species are put into the spare test tube equipped with nutrient solution, stopper tampon after be placed on side, successively will examination Parent species sorting in pipe finishes;
C. Initial strain breeding, will equipped with purification parent species test tube be put into incubator, temperature setting be 15 DEG C -20 DEG C culture after a week, It observes the growing state of parent species, eliminate the parent species for infecting miscellaneous bacteria, while cultivating parent species, should prepare to breed original seed as early as possible Raw material, the raw material of original seed are bulky grain sawdust 70%, wheat bran 28%, gypsum 1%, sugared 1%, appropriate amount of water, first by the original of original seed Original seed bacteria stick is put into high-pressure sterilizing pot after being packed, being packed using sack filling machine after being sufficiently stirred, is added by material Heated after suitable quantity of water, sealed pot cover, should keep taking the dish out of the pot after 6h, sterilizing when autoclave air pressure inside reaches 2KPa it is cold But to room temperature, sterilized original seed bacteria stick and parent species is transferred in the transfer room through strict sterilization, are then placed in inoculating hood, by skill Parent species are sequentially ingressed among the bacteria stick of original seed by art personnel according to operating instruction, are inoculated with the original seed bacteria stick finished and are transferred into culturing room Cultivated, the temperature of culturing room is maintained between 15 DEG C -20 DEG C, air humidity between 50%-60%, interior needs through normal open Wind ventilation;
D. the breeding of cultivar, original seed should often stir bacteria stick when culturing room is cultivated and be observed, find that mycelia turns to be yellow Or black bacteria stick be infected miscellaneous bacteria bacteria stick meet an urgent need when eliminate, can be carried out after mycelia is covered with 80% position of bacteria stick Inoculation, prepares the raw material of cultivar when cultivating the later period emergency of original seed, the raw material of cultivar be sawdust 80%, wheat bran 18%, Gypsum 1%, sugared 1%, appropriate amount of water are packed using sack filling machine after being sufficiently stirred the raw material of cultivar, pack and finish Cultivar bacteria stick is put into normal-pressure sterilization room afterwards, is generated after the temperature inside sterilization chamber is risen to 100 DEG C by steam and is protected using boiler Hold and carry out high-temperature sterilization for 24 hours, be cooled to room temperature the transfer room that bacteria stick moves to strict sterilization after sterilizing, by original seed and Sterilized cultivar bacteria stick is put into inoculating hood, original seed is sequentially ingressed into the bacteria stick of cultivar by technical staff according to operating instruction Among, be inoculated with the cultivar bacteria stick finished be transferred into culturing room cultivated, the temperature of culturing room be maintained at 15 DEG C -20 DEG C it Between, air humidity between 50%-60%, the frequent ventilation of indoor needs, can be with after mycelia is covered with 80% position of bacteria stick The bacteria stick of growth black fungus is inoculated with.
CN201811073253.6A 2018-09-14 2018-09-14 Fang County's local ecosystem edible fungus species raising technology Withdrawn CN109042075A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110115199A (en) * 2019-06-05 2019-08-13 广西壮族自治区农业科学院微生物研究所 A method of production cloud ear strain is considered to be worth doing with Eucalyptus

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CN107409750A (en) * 2017-05-22 2017-12-01 黑龙江省润成新能源科技发展有限公司 A kind of method using beneficial bacteria willow cultivating black fungus
CN107667776A (en) * 2017-09-29 2018-02-09 山西禾嘉合信息科技有限公司 A kind of Household edible mushroom planting method
CN107750814A (en) * 2017-11-08 2018-03-06 贺州市金子山生态灵芝林下种植专业合作社 A kind of breeding method of Wild ganoderma strain

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110115199A (en) * 2019-06-05 2019-08-13 广西壮族自治区农业科学院微生物研究所 A method of production cloud ear strain is considered to be worth doing with Eucalyptus

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