CN107750814A - A kind of breeding method of Wild ganoderma strain - Google Patents
A kind of breeding method of Wild ganoderma strain Download PDFInfo
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Abstract
The present invention provides a kind of breeding method of Wild ganoderma strain, is related to mushroom cultivation technical field, the breeding method of the Wild ganoderma strain comprises the following steps：(1) preparation of tissue culture medium (TCM), (two), which obtain, cultivates kind, the cultivation of (three) test tube kind, the cultivation of (four) Wild ganoderma strain；The present invention is used as cultivation kind by choosing Wild ganoderma, kind is cultivated again to cultivate to obtain test tube kind by test tube, it is changed into original seed in test tube again, by original seed again to parent species, finally master clock is accessed on wood to cultivate at a constant temperature and obtain cultigen until covering with mycelia, the present invention is very rigorous from selection to cultivating process, the repetition for avoiding cultivation strain is cultivated, improve culture medium prescription, optimize cultivating technique, the degeneration of strain is not easily caused, mycelium is strong, anti- miscellaneous bacteria performance is good, the strain vitality cultivated using the method for the present invention is indomitable, and medical value is high, it can be laid a good foundation for the cultivating superior high-yield of ganoderma lucidum.
Description
【Technical field】
The present invention relates to mushroom cultivation technical field, and in particular to a kind of breeding method of Wild ganoderma strain.
【Background technology】
Ganoderma lucidum is also known as red sesame, grass of returning to life from the nether world, and fungi Ganoderma, is made up of cap and stem, and smell is fragrant, bitter and puckery flavor.It is modern
Medical evidence, ganoderma lucidum contain several physiological active substances, can adjust, strengthen body immunity, and neurasthenia, rheumatic are closed
Section inflammation, coronary heart disease, hypertension etc. have good synergistic therapeutic action, in addition, also with antifatigue, beautifying face and moistering lotion, delay
Aging, the effect of preventing and treating a variety of diseases.
Strain is the basis of ganoderma lucidum production, and the excellent kind of seed selection is to ensure the important step of ganoderma lucidum good quality and high output, is refined
Wild ganoderma strain, obtained ganoderma lucidum drug effect are higher than the ganoderma lucidum that greenhouse is cultivated, however, the wild Ganoderma from nature collection
Kind, although can also carry out artificial cultivation, particular/special requirement of the industrialization large-scale production Garnoderma product to strain can not be met,
Including：Fermentation condition, culture medium, temperature, humidity, and the life of its synthesis to matrix, conversion, degraded etc. during the fermentation
Manage Biochemical changes.Therefore Wild ganoderma strain must be cultivated using special mutation breeding technologies, in the hope of obtaining excellent life
Strain is produced to meet large-scale production requirement.
【The content of the invention】
In view of this, it is of the invention from choosing object of the present invention is to provide a kind of breeding method of Wild ganoderma strain
Material is very rigorous to cultivating process, and the repetition for avoiding cultivation strain is cultivated, and is improved culture medium prescription, is optimized cultivation work
Skill, the degeneration of strain is not easily caused, mycelium is strong, anti-miscellaneous bacteria performance is good, the strain life cultivated using the method for the present invention
Power is indomitable, and medical value is high, can be that the cultivating superior high-yield of ganoderma lucidum is laid a good foundation.
In order to solve the above-mentioned technical problem, present invention employs following technical scheme：
A kind of breeding method of Wild ganoderma strain, the breeding method of the Wild ganoderma strain comprise the following steps：
(1) preparation of tissue culture medium (TCM)
The tissue culture medium (TCM) is made up of the raw material of following parts by weight：Soya-bean cake 30-40 parts, rice bran 15-25 parts, wheat bran 10-
15 parts, sucrose 10-15 parts, land plaster 20-25 parts, soybean stalk 10-15 parts, agar 3-8 parts, boric acid 1-5 parts, forchlorfenuron 5-
9 parts, rape element sterol 5-10 parts and water 80-100 parts；
The configuration of the tissue culture medium (TCM) is disinfection at being then 95-110 DEG C in temperature after above-mentioned raw materials are mixed
25-35 hours, you can it is standby to obtain tissue culture medium (TCM)；
(2) obtain and cultivate kind
Wild ganoderma is found from remote mountains, takes robust growth, no disease and pests harm, the young tender stem or cap for not yet forming cap
The fructification grown, the yellowish-white position grown is torn, with the cap former base or cap at the top of transfer needle picking stem
The fritter of tender tissue one at edge, as cultivating, kind is standby；
(3) cultivation of test tube kind
Tissue culture medium (TCM) is sub-packed in the test tube after sterilizing, obtains Tube propagation base, will be disappeared by high temperature
Cultivation kind after poison is seeded on Tube propagation base inclined-plane, is carried out inclined-plane culture 10-15 days at being 25-28 DEG C in temperature, is worked as bacterium
Filament length stops culture when expiring test tube, then carries out low-temperature preservation, and it is standby to obtain test tube kind；
(4) cultivation of Wild ganoderma strain
A. breading protospecies：The Chinese medicine of the weed tree sawdust of 30-40 parts, the wheat bran of 15-20 parts, 5-10 parts is taken to remain slag, 5-10 parts
Sugar and the land plaster of 2-4 parts are thoroughly mixed uniformly and then added suitable quantity of water and adjust humidity of materials to 45-55%, obtain
Culture matrix A, it is 5-6 that pH adjusting agent regulation pH is then added in culture matrix A, connects test tube kind after high-temperature sterilization cooling
Enter, grown 30-45 days at 15-25 DEG C, obtain original seed；B. parent species are cultivated：The raw material of following parts by weight is mixed to get culture
Matrix B：Weed tree sawdust 30-40 parts, wheat bran 15-20 parts, agricultural crop straw 10-15 parts, brown sugar 5-10 parts, Chinese medicine remain slag 5-10 parts,
Urea 1-3 parts, -4 parts of Riboflavin Tetrabutyrate, land plaster 2-4 parts and appropriate amount of water, culture matrix B is fitted into bag, again will after carrying out disinfection
Obtained original seed is put into the sack after sterilization, is cultivated 30-50 days under conditions of 20-30 DEG C, with the growth of mycelia, bacterium colony
Extension, pick out that ganoderma lucidum mycelium growth is neat, long speed is normal, mycelia outward appearance is full, growing way is vigorous, aerial hyphae becomes sworn brothers or sisters,
Very thin, fine and close, uniform part is as parent species；C. cultigen is cultivated：Parent species are connected on wood, cultivated up to covering with mycelia,
The cultivation of Wild ganoderma strain can be completed.
In the present invention, further, the tissue culture medium (TCM) is made up of the raw material of following parts by weight：35 parts of soya-bean cake, rice bran
20 parts, 12 parts of wheat bran, 12 parts of sucrose, 22 parts of land plaster, 12 parts of soybean stalk, 6 parts of agar, 3 parts of boric acid, 7 parts of forchlorfenuron, oil
90 parts of 7 parts of dish element sterol and water.
In the present invention, further, the step (1) is first passed through and boiled before the disinfection of tissue culture medium (TCM) is carried out
The pH of tissue culture medium (TCM) is adjusted to 5.0-6.0.
In the present invention, further, culture matrix A in the step (4) in breading protospecies by following parts by weight original
Material composition：35 parts of weed tree sawdust, 17 parts of wheat bran, Chinese medicine remain 7 parts of slag, sugared 7 parts and 3 parts of land plaster.
In the present invention, further, in the step (4) parent species cultivate in culture matrix B by following parts by weight original
Material composition：35 parts of weed tree sawdust, 17 parts of wheat bran, 12 parts of agricultural crop straw, 7 parts of brown sugar, Chinese medicine remain 7 parts of slag, 2 parts of urea, riboflavin 3
Part, 3 parts of land plaster and appropriate amount of water.
In the present invention, further, the cultivation temperature that the cultigen in the step (4) is cultivated is the cultivation of first three day
Temperature is 27-30 DEG C, and cultivation temperature afterwards is maintained between 20-26 DEG C.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1st, in tissue culture medium (TCM) of the invention, soya-bean cake is made as nitrogen source, sucrose, rice bran and wheat bran as carbon source, land plaster
For inorganic salts, ensure although to be not easy to be dissolved rich in trace element, wheat bran and rice bran in culture medium, but it is easily broken be cleaved into glucose and
Glucoside, it is also more suitable for the needs of mycelial growth, is additionally provided with the factor needed for ganoderma lucidum culture kind growth, mitogen needs growing
Competence exertion acts in the presence of element, and forchlorfenuron therein is mitogen, rape element sterol is auxin interaction,
Ensure that forchlorfenuron gives full play to its effect in the case where there is auxin, accelerate plant cell division, promote mycelial growth.
2nd, the present invention is used as cultivation kind by choosing Wild ganoderma, then cultivates kind and cultivate to obtain test tube kind by test tube, tries
Guan Zhongzai is changed into original seed, by original seed again to parent species, finally accesses on wood master clock and cultivates at a constant temperature until covering with mycelia
Cultigen is obtained, the present invention is very rigorous from selection to cultivating process, and the repetition for avoiding cultivation strain is cultivated, and is improved
Culture medium prescription, optimizes cultivating technique, does not easily cause the degeneration of strain, and mycelium is strong, anti-miscellaneous bacteria performance is good, using the present invention
The strain vitality cultivated of method it is indomitable, and medical value is high, can lay good base for the cultivating superior high-yield of ganoderma lucidum
Plinth.
【Embodiment】
The following examples can help those skilled in the art that the present invention is more fully understood, but cannot be with any
Mode limits the present invention.
Embodiment 1
A kind of breeding method for Wild ganoderma strain that the present embodiment provides, comprises the following steps：
(1) preparation of tissue culture medium (TCM)
Tissue culture medium (TCM) is made up of the raw material of following parts by weight：30 parts of soya-bean cake, 15 parts of rice bran, 10 parts of wheat bran, 10 parts of sucrose,
80 parts of 20 parts of land plaster, 10 parts of soybean stalk, 3 parts of agar, 1 part of boric acid, 5 parts of forchlorfenuron, 5 parts of rape element sterol and water；Group
The configuration for knitting culture medium is to first pass through to boil to adjust the pH of tissue culture medium (TCM) to 5.0, then in temperature after above-mentioned raw materials are mixed
Spend for disinfection at 95 DEG C 25 hours, you can it is standby to obtain tissue culture medium (TCM)；
(2) obtain and cultivate kind
Wild ganoderma is found from remote mountains, takes robust growth, no disease and pests harm, the young tender stem or cap for not yet forming cap
The fructification grown, the yellowish-white position grown is torn, with the cap former base or cap at the top of transfer needle picking stem
The fritter of tender tissue one at edge, as cultivating, kind is standby；
(3) cultivation of test tube kind
Tissue culture medium (TCM) is sub-packed in the test tube after sterilizing, obtains Tube propagation base, will be disappeared by high temperature
Cultivation kind after poison is seeded on Tube propagation base inclined-plane, inclined-plane culture is carried out 10 days at being 25 DEG C in temperature, when mycelia is covered with
Stop culture during test tube, then carry out low-temperature preservation, it is standby to obtain test tube kind；
(4) cultivation of Wild ganoderma strain
A. breading protospecies：30 parts of weed tree sawdust, 15 parts of wheat bran, 5 parts of Chinese medicine is taken to remain slag, 5 parts of sugar and 2 parts of stone
Cream powder is thoroughly mixed uniformly and then added suitable quantity of water and adjusts humidity of materials to 45%, obtains culture matrix A, is then training
It is 5 to support and pH adjusting agent regulation pH is added in matrix A, accesses test tube kind after high-temperature sterilization cooling, grows 30 days, obtain at 15 DEG C
To original seed；B. parent species are cultivated：The raw material of following parts by weight is mixed to get culture matrix B：30 parts of weed tree sawdust, 150 parts of wheat bran, agriculture
10 parts of crop material, 5 parts of brown sugar, Chinese medicine remain 5 parts of slag, 1 part of urea, Riboflavin Tetrabutyrate part, 2 parts of land plaster and appropriate amount of water, by culture medium
Matter B is fitted into bag, and obtained original seed is put into the sack after sterilization again after carrying out disinfection, 30 are cultivated under conditions of 20 DEG C
My god, with the growth of mycelia, the extension of bacterium colony, pick out ganoderma lucidum mycelium growth is neat, long speed is normal, mycelia outward appearance is full,
Growing way is vigorous, and aerial hyphae is become sworn brothers or sisters, be very thin, be fine and close, uniform part is as parent species；C. cultigen is cultivated：Parent species are connected to wood
On head, cultivate until covering with mycelia, it is 27 DEG C to cultivate the cultivation temperature that temperature is first three day, and cultivation temperature afterwards is maintained at 20
Between DEG C, you can complete the cultivation of Wild ganoderma strain.
Embodiment 2
A kind of breeding method for Wild ganoderma strain that the present embodiment provides, comprises the following steps：
(1) preparation of tissue culture medium (TCM)
Tissue culture medium (TCM) is made up of the raw material of following parts by weight：35 parts of soya-bean cake, 20 parts of rice bran, 12 parts of wheat bran, 12 parts of sucrose,
90 parts of 22 parts of land plaster, 12 parts of soybean stalk, 6 parts of agar, 3 parts of boric acid, 7 parts of forchlorfenuron, 7 parts of rape element sterol and water；Group
The configuration for knitting culture medium is to first pass through to boil to adjust the pH of tissue culture medium (TCM) to 5.5, then in temperature after above-mentioned raw materials are mixed
Spend for disinfection at 100 DEG C 30 hours, you can it is standby to obtain tissue culture medium (TCM)；
(2) obtain and cultivate kind
Wild ganoderma is found from remote mountains, takes robust growth, no disease and pests harm, the young tender stem or cap for not yet forming cap
The fructification grown, the yellowish-white position grown is torn, with the cap former base or cap at the top of transfer needle picking stem
The fritter of tender tissue one at edge, as cultivating, kind is standby；
(3) cultivation of test tube kind
Tissue culture medium (TCM) is sub-packed in the test tube after sterilizing, obtains Tube propagation base, will be disappeared by high temperature
Cultivation kind after poison is seeded on Tube propagation base inclined-plane, inclined-plane culture is carried out 12 days at being 27 DEG C in temperature, when mycelia is covered with
Stop culture during test tube, then carry out low-temperature preservation, it is standby to obtain test tube kind；
(4) cultivation of Wild ganoderma strain
A. breading protospecies：35 parts of weed tree sawdust, 17 parts of wheat bran, 7 parts of Chinese medicine is taken to remain slag, 7 parts of sugar and 3 parts
Land plaster is thoroughly mixed uniformly and then added suitable quantity of water and adjusts humidity of materials to 50%, obtains culture matrix A, Ran Hou
It is 5.5 that pH adjusting agent regulation pH is added in culture matrix A, accesses test tube kind after high-temperature sterilization cooling, grows 38 at 20 DEG C
My god, obtain original seed；B. parent species are cultivated：The raw material of following parts by weight is mixed to get culture matrix B：35 parts of weed tree sawdust, wheat bran 17
Part, 12 parts of agricultural crop straw, 7 parts of brown sugar, Chinese medicine remain 7 parts of slag, 2 parts of urea, 3 parts of riboflavin, 3 parts of land plaster and appropriate amount of water, will
Culture matrix B is fitted into bag, and obtained original seed is put into the sack after sterilization again after carrying out disinfection, trained under conditions of 25 DEG C
Support 40 days, with the growth of mycelia, the extension of bacterium colony, pick out that ganoderma lucidum mycelium growth is neat, long speed is normal, mycelia outward appearance is satisfied
Completely, growing way is vigorous, and aerial hyphae is become sworn brothers or sisters, be very thin, be fine and close, uniform part is as parent species；C. cultigen is cultivated：Parent species are connected to
On wood, cultivate until covering with mycelia, it is 28 DEG C to cultivate the cultivation temperature that temperature is first three day, and cultivation temperature afterwards is maintained at
Between 23 DEG C, you can complete the cultivation of Wild ganoderma strain.
Embodiment 3
A kind of breeding method for Wild ganoderma strain that the present embodiment provides, comprises the following steps：
(1) preparation of tissue culture medium (TCM)
Tissue culture medium (TCM) is made up of the raw material of following parts by weight：40 parts of soya-bean cake, 25 parts of rice bran, 15 parts of wheat bran, 15 parts of sucrose,
100 parts of 25 parts of land plaster, 15 parts of soybean stalk, 8 parts of agar, 5 parts of boric acid, 9 parts of forchlorfenuron, 10 parts of rape element sterol and water；
The configuration of tissue culture medium (TCM) is to first pass through to boil to adjust the pH of tissue culture medium (TCM) to 6.0, Ran Hou after above-mentioned raw materials are mixed
Temperature is disinfection 35 hours at 110 DEG C, you can it is standby to obtain tissue culture medium (TCM)；
(2) obtain and cultivate kind
Wild ganoderma is found from remote mountains, takes robust growth, no disease and pests harm, the young tender stem or cap for not yet forming cap
The fructification grown, the yellowish-white position grown is torn, with the cap former base or cap at the top of transfer needle picking stem
The fritter of tender tissue one at edge, as cultivating, kind is standby；
(3) cultivation of test tube kind
Tissue culture medium (TCM) is sub-packed in the test tube after sterilizing, obtains Tube propagation base, will be disappeared by high temperature
Cultivation kind after poison is seeded on Tube propagation base inclined-plane, inclined-plane culture is carried out 15 days at being 28 DEG C in temperature, when mycelia is covered with
Stop culture during test tube, then carry out low-temperature preservation, it is standby to obtain test tube kind；
(4) cultivation of Wild ganoderma strain
A. breading protospecies：40 parts of weed tree sawdust, 20 parts of wheat bran, 10 parts of Chinese medicine is taken to remain slag, 10 parts of sugar and 4 parts
Land plaster is thoroughly mixed uniformly and then added suitable quantity of water and adjusts humidity of materials to 55%, obtains culture matrix A, Ran Hou
It is 6 that pH adjusting agent regulation pH is added in culture matrix A, accesses test tube kind after high-temperature sterilization cooling, is grown 45 days at 25 DEG C,
Obtain original seed；B. parent species are cultivated：The raw material of following parts by weight is mixed to get culture matrix B：40 parts of weed tree sawdust, 20 parts of wheat bran,
15 parts of agricultural crop straw, 10 parts of brown sugar, Chinese medicine remain 10 parts of slag, 3 parts of urea, 4 parts of riboflavin, 4 parts of land plaster and appropriate amount of water, will train
Support matrix B to be fitted into bag, obtained original seed is put into the sack after sterilization again after carrying out disinfection, cultivated under conditions of 30 DEG C
50 days, with the growth of mycelia, the extension of bacterium colony, pick out that ganoderma lucidum mycelium growth is neat, long speed is normal, mycelia outward appearance is satisfied
Completely, growing way is vigorous, and aerial hyphae is become sworn brothers or sisters, be very thin, be fine and close, uniform part is as parent species；C. cultigen is cultivated：Parent species are connected to
On wood, cultivate until covering with mycelia, it is 30 DEG C to cultivate the cultivation temperature that temperature is first three day, and cultivation temperature afterwards is maintained at
Between 26 DEG C, you can complete the cultivation of Wild ganoderma strain.
Compliance test result
In order to further illustrate the practical value of implantation methods of the present invention, contrast test is done：Five groups of observations below point
The average day growth speed of Ganoderma lucidum mycelium：First group：The methods described of embodiment 1；Second group：The methods described of embodiment 2；3rd group：
The methods described of embodiment 3；4th group：Tissue culture medium (TCM) is changed to：35% agar, 25% glucose, 6% calcium superphosphate, 34%
Wheat bran, other method are carried out in strict accordance with embodiment 2；5th group：Wild cultivation kind is changed to greenhouse and cultivates kind, other method is tight
Lattice are carried out according to embodiment 2；
Situation record such as following table：
The longan of table 1 plants quality versus's table
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (6)
1. a kind of breeding method of Wild ganoderma strain, it is characterised in that the breeding method of the Wild ganoderma strain is included such as
Lower step：
(1) preparation of tissue culture medium (TCM)
The tissue culture medium (TCM) is made up of the raw material of following parts by weight：Soya-bean cake 30-40 parts, rice bran 15-25 parts, wheat bran 10-15 parts,
Sucrose 10-15 parts, land plaster 20-25 parts, soybean stalk 10-15 parts, agar 3-8 parts, boric acid 1-5 parts, forchlorfenuron 5-9 parts,
Rape element sterol 5-10 parts and water 80-100 parts；
The configuration of the tissue culture medium (TCM) is disinfection 25- at being then 95-110 DEG C in temperature after above-mentioned raw materials are mixed
35 hours, you can it is standby to obtain tissue culture medium (TCM)；
(2) obtain and cultivate kind
Wild ganoderma is found from remote mountains, robust growth, no disease and pests harm is taken, is not yet forming the young tender stem or cap of cap
The fructification of growth, the yellowish-white position grown is torn, with the cap former base or cap edge at the top of transfer needle picking stem
The fritter of tender tissue one, it is kind standby as cultivating；
(3) cultivation of test tube kind
Tissue culture medium (TCM) is sub-packed in the test tube after sterilizing, obtains Tube propagation base, will be after high-temperature sterilization
Cultivation kind be seeded on Tube propagation base inclined-plane, inclined-plane culture 10-15 days is carried out at being 25-28 DEG C in temperature, when mycelia grows
Stop culture during full test tube, then carry out low-temperature preservation, it is standby to obtain test tube kind；
(4) cultivation of Wild ganoderma strain
A. breading protospecies：Take the weed tree sawdust of 30-40 parts, the wheat bran of 15-20 parts, 5-10 parts Chinese medicine remain slag, 5-10 parts sugar with
And the land plaster of 2-4 parts is thoroughly mixed uniformly and then added suitable quantity of water and adjusts humidity of materials to 45-55%, is cultivated
Matrix A, it is 5-6 that pH adjusting agent regulation pH is then added in culture matrix A, accesses test tube kind after high-temperature sterilization cooling,
Grown 30-45 days at 15-25 DEG C, obtain original seed；B. parent species are cultivated：The raw material of following parts by weight is mixed to get culture matrix B：
Weed tree sawdust 30-40 parts, wheat bran 15-20 parts, agricultural crop straw 10-15 parts, brown sugar 5-10 parts, Chinese medicine remain slag 5-10 parts, urea 1-3
Part, -4 parts of Riboflavin Tetrabutyrate, land plaster 2-4 parts and appropriate amount of water, culture matrix B are fitted into bag, the original that will be obtained again after carrying out disinfection
Kind it is put into the sack after sterilization, is cultivated 30-50 days under conditions of 20-30 DEG C, with the growth of mycelia, the extension of bacterium colony,
Pick out that ganoderma lucidum mycelium growth is neat, long speed is normal, mycelia outward appearance is full, growing way is vigorous, aerial hyphae becomes sworn brothers or sisters, be very thin, causing
Close, uniform part is as parent species；C. cultigen is cultivated：Parent species are connected on wood, cultivated until covering with mycelia, you can complete
The cultivation of Wild ganoderma strain.
A kind of 2. breeding method of Wild ganoderma strain according to claim 1, it is characterised in that the tissue culture medium (TCM)
It is made up of the raw material of following parts by weight：35 parts of soya-bean cake, 20 parts of rice bran, 12 parts of wheat bran, 12 parts of sucrose, 22 parts of land plaster, big beanstalk
90 parts of 12 parts of stalk, 6 parts of agar, 3 parts of boric acid, 7 parts of forchlorfenuron, 7 parts of rape element sterol and water.
3. the breeding method of a kind of Wild ganoderma strain according to claim 1, it is characterised in that the step (1) exists
Before the disinfection for carrying out tissue culture medium (TCM), first pass through to boil and adjust the pH of tissue culture medium (TCM) to 5.0-6.0.
4. the breeding method of a kind of Wild ganoderma strain according to claim 1, it is characterised in that in the step (4)
Culture matrix A in breading protospecies is made up of the raw material of following parts by weight：Surplus 7 parts of the slag of 35 parts of weed tree sawdust, 17 parts of wheat bran, Chinese medicine,
Sugared 7 parts and 3 parts of land plaster.
5. the breeding method of a kind of Wild ganoderma strain according to claim 1, it is characterised in that in the step (4)
Culture matrix B during parent species are cultivated is made up of the raw material of following parts by weight：35 parts of weed tree sawdust, 17 parts of wheat bran, agricultural crop straw 12
Part, 7 parts of brown sugar, Chinese medicine remain 7 parts of slag, 2 parts of urea, 3 parts of riboflavin, 3 parts of land plaster and appropriate amount of water.
6. the breeding method of a kind of Wild ganoderma strain according to claim 1, it is characterised in that in the step (4)
The cultivation temperature cultivated of cultigen be that the cultivation temperature of first three day is 27-30 DEG C, cultivation temperature afterwards is maintained at 20-26 DEG C
Between.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042075A (en) * | 2018-09-14 | 2018-12-21 | 段家忠 | Fang County's local ecosystem edible fungus species raising technology |
| CN109673380A (en) * | 2018-12-13 | 2019-04-26 | 浙江渊健生物药业有限公司 | A kind of field implantation methods of dragon's fountain ganoderma lucidum |
| CN111788988A (en) * | 2020-05-19 | 2020-10-20 | 石狮昌隆农业种植有限公司 | Method and device for efficiently and artificially cultivating lucid ganoderma |
| CN113179855A (en) * | 2021-03-31 | 2021-07-30 | 山东中泰药业有限公司 | Traditional Chinese medicine residue ganoderma lucidum culture medium and preparation method thereof |
-
2017
- 2017-11-08 CN CN201711091249.8A patent/CN107750814A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042075A (en) * | 2018-09-14 | 2018-12-21 | 段家忠 | Fang County's local ecosystem edible fungus species raising technology |
| CN109673380A (en) * | 2018-12-13 | 2019-04-26 | 浙江渊健生物药业有限公司 | A kind of field implantation methods of dragon's fountain ganoderma lucidum |
| CN111788988A (en) * | 2020-05-19 | 2020-10-20 | 石狮昌隆农业种植有限公司 | Method and device for efficiently and artificially cultivating lucid ganoderma |
| CN113179855A (en) * | 2021-03-31 | 2021-07-30 | 山东中泰药业有限公司 | Traditional Chinese medicine residue ganoderma lucidum culture medium and preparation method thereof |
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