CN1316007C - Outer space carrying device for gene engineering cell - Google Patents
Outer space carrying device for gene engineering cell Download PDFInfo
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- CN1316007C CN1316007C CNB2004100088292A CN200410008829A CN1316007C CN 1316007 C CN1316007 C CN 1316007C CN B2004100088292 A CNB2004100088292 A CN B2004100088292A CN 200410008829 A CN200410008829 A CN 200410008829A CN 1316007 C CN1316007 C CN 1316007C
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- engineered cell
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Abstract
The present invention relates to an outer space carrying device for gene engineering cells, which comprises a vessel (2), a cover (1) on the vessel, culture liquid (4) in the vessel and a micro carrier (3) with holes, wherein the micro carrier (3) is soaked in the culture liquid. The outer space carrying device for gene engineering cells is characterized in that the micro carrier (3) with holes is a non-poisonous high molecular compound selected from cellulose microcrystalline, chitin or collogen and is provided with the holes with the diameter of 20 to 60 microns, and the culture liquid (4) is a culture medium added with glutamine and hydrocortisone. The outer space carrying device for gene engineering cells can maintain the survival of the cells for 30 days at the normal temperature of 4 to 37 DEG C under the conditions of no power supply, no heat source, no oxygen, no carbon dioxide supply and no need of the replacement of the culture medium. After the cells go back at the normal temperature of 4 to 37 DEG C, the cells can continuously grow and propagate at the temperature of 36.5 (+/-) 0.5 DEG C.
Description
Technical field
The present invention relates to the space treatment device of genetically engineered cell, particularly related to and be suitable for utilizing retrievable satellite to carry out the device of the genetically engineered cell long-term surviving of passive lift-launch.
Background technology
Space environment has many characteristics different with earth surface, and as microgravity, little magnetic field, irradiation space etc., these characteristics can have many-sided influence to life on earth.China utilized retrievable satellite to carry plant seed from 1987, through space environment selection by mutation, now cultivated the higher yield of crops strain of multiple good quality and high output on ground.Retrievable satellite is carried with multiple microbiotic bacterial classification in China, and microbiotic output obviously improves.The cynnematin acylase gene engineering strain lift-launch retrievable satellite that wherein will be in the propagation phase in 1994 carries out space mutagenesis, filters out a plant mutant bacterial strain behind the ground return, and acylase improves 4.35 times.Because passive lift-launch retrievable satellite is difficult to provide the mammalian cell life condition, carry retrievable satellite so limited the mammal genetically engineered cell, carry out space mutagenesis, improve the research and the application of genetically engineered drug output.
Goal of the invention of the present invention is to overcome above-mentioned defective, and a kind of space treatment device of easy, cheap, easy-operating genetically engineered cell is provided.
Summary of the invention
The space treatment device of genetically engineered cell of the present invention, wherein: it comprises: the nutrient solution in the lid on container, the container, container and be immersed in tool hole microcarrier in the nutrient solution, described tool hole microcarrier is to be selected from Mierocrystalline cellulose, chitin or collagen, it is the hole of 20-60 micron that diameter is arranged on it, described nutrient solution is the substratum that is added with glutamine and hydrocortisone, and described substratum is any in Dulbecco modified form substratum, Ham-F12 substratum and RPMI 1640 substratum.
The space treatment device of genetically engineered cell of the present invention, wherein: the addition of described glutamine is to add the 0.5-3 gram in every liter of nutrient solution.
The space treatment device of genetically engineered cell of the present invention, wherein: the addition of described hydrocortisone is to add the 0.5-1.5 milligram in every liter of nutrient solution.
The space treatment device of genetically engineered cell of the present invention, wherein: described tool hole microcarrier is a globular.
The space treatment device of genetically engineered cell of the present invention, wherein: the spherical diameter of described tool hole microcarrier is the 100-500 micron.
The space treatment device of genetically engineered cell of the present invention, wherein: the lid on described container and the container is that the screw thread by thereon seals.
The space treatment device of genetically engineered cell of the present invention, wherein: the lid on described container and the container is to be made by the resilient material of aseptic no cytotoxicity.
The space treatment device of genetically engineered cell of the present invention, wherein: the lid on described container and the container is made by polypropylene material.
Compare with the space treatment device of existing genetically engineered cell, advantage of the present invention is as follows:
Device of the present invention can be in non-transformer, no thermal source, no oxygen, the supply of no carbonic acid gas, need not to change under the condition of substratum, keep cell and reach 30 days 4-37 ℃ of normal temperature survival, after cell returns from 4-37 ℃ of normal temperature, but continued growth breeding under 36.5 ± 0.5 ℃ temperature.
Description of drawings
Fig. 1 is the forward sectional view of the space treatment device of genetically engineered cell of the present invention;
Fig. 2 is the enlarged diagram of tool hole microcarrier of the space treatment device of genetically engineered cell of the present invention;
Fig. 3 is that 30 days CHO-dhfr-cell of existence amplifies 200 times synoptic diagram in device of the present invention;
Fig. 4 is with the CHO-dhfr of device of the present invention through space treatment
-Cell amplifies 100 times synoptic diagram.
Embodiment
Referring to Fig. 1, the space treatment device of genetically engineered cell comprises: the nutrient solution 4 in the lid 1 on container 2, the container, container and be immersed in tool hole microcarrier 3 in the nutrient solution, tool hole microcarrier 3 is the non-toxic high-molecular compounds that are selected from Mierocrystalline cellulose, chitin or collagen, it is the hole (referring to Fig. 2) of 20-60 micron that diameter is arranged on it, it can be globular or other shape, if the globular words, its spherical diameter is the 100-500 micron; Nutrient solution 4 is the substratum that are added with glutamine and hydrocortisone, wherein the addition of glutamine is to add the 0.5-3 gram in every liter of nutrient solution, the addition of hydrocortisone is to add the 0.5-1.5 milligram in every liter of nutrient solution, and described substratum is to use any in Dulbecco modified form substratum, Ham-F12 substratum and the RPMI1640 substratum commonly used, lid 1 on container 2 and the container by the resilient material of aseptic no cytotoxicity for example is: polypropylene is made, and they are that screw thread by thereon seals.
Embodiment 1:
Shown in Fig. 1-2, this apparatus container is to adopt the frozen pipe of being made by polypropylene material 2, and synthetic nutrient solution 4 is housed in it, and this synthetic nutrient solution is to add glutamine by Ham-F12 substratum commonly used with in above-mentioned nutrient solution in every liter of ratio that adds 2 grams; In every liter of hydrocortisone that adds 1 milligram ratio adding, in described synthetic nutrient solution, be soaked with tool hole microcarrier 3, the particulate composition is a Mierocrystalline cellulose, mean particle dia is 230 microns, the aperture is 30 microns, adds Tetrahydrofolate dehydrogenase defective type Chinese hamster ovary cell (CHO-dhfr in said apparatus
-) after, screw-in covers 1 and seals, and keep cell and survived 30 days under 4-37 ℃ of normal temperature, but the breeding of continued growth is after this seen shown in Figure 3.
Embodiment 2:
Shown in Fig. 1-2, this apparatus container is to adopt the frozen pipe of being made by polypropylene material 2, and synthetic nutrient solution 4 is housed in it, and this synthetic nutrient solution is to add glutamine by Ham-F12 substratum commonly used with in above-mentioned nutrient solution in every liter of ratio that adds 2 grams; In every liter of hydrocortisone that adds 1 milligram ratio adding, in described synthetic nutrient solution, be soaked with tool hole microcarrier 3, the particulate composition is a Mierocrystalline cellulose, mean particle dia is 230 microns, the aperture is 30 microns, adds Tetrahydrofolate dehydrogenase defective type Chinese hamster ovary cell (CHO-dhfr in said apparatus
-) after, screw in and cover 1 and seal, carry China's the 18th st recoverable satellite, through space flight in 17 days and surface detention 8 days, kept cell survival altogether 25 days, this cell after 4-37 ℃, but continued growth breed, see shown in Figure 4.
Claims (8)
1. the space treatment device of a genetically engineered cell, it comprises: the nutrient solution (4) in the lid (1) on container (2), the container, container and be immersed in tool hole microcarrier (3) in the nutrient solution, it is characterized in that: described tool hole microcarrier (3) is to be selected from Mierocrystalline cellulose, chitin or collagen, it is the hole of 20-60 micron that diameter is arranged on it, described nutrient solution (4) is the substratum that is added with glutamine and hydrocortisone, and described substratum is any in Dulbecco modified form substratum, Ham-F12 substratum and the RPMI1640 substratum.
2. the space treatment device of a kind of genetically engineered cell as claimed in claim 1 is characterized in that: the addition of described glutamine is to add the 0.5-3 gram in every liter of nutrient solution.
3. the space treatment device of a kind of genetically engineered cell as claimed in claim 1 is characterized in that: the addition of described hydrocortisone is to add the 0.5-1.5 milligram in every liter of nutrient solution.
4. as the space treatment device of claim 2 or 3 described a kind of genetically engineered cells, it is characterized in that: described tool hole microcarrier (3) is a globular.
5. the space treatment device of a kind of genetically engineered cell as claimed in claim 4, it is characterized in that: the spherical diameter of described tool hole microcarrier (3) is the 100-500 micron.
6. the space treatment device of a kind of genetically engineered cell as claimed in claim 5 is characterized in that: the lid (1) on described container (2) and the container is that the screw thread by thereon seals.
7. the space treatment device of a kind of genetically engineered cell as claimed in claim 6, it is characterized in that: the lid (1) on described container (2) and the container is to be made by the resilient material of aseptic no cytotoxicity.
8. the space treatment device of a kind of genetically engineered cell as claimed in claim 6, it is characterized in that: the lid (1) on described container (2) and the container is made by polypropylene material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100088292A CN1316007C (en) | 2004-03-18 | 2004-03-18 | Outer space carrying device for gene engineering cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100088292A CN1316007C (en) | 2004-03-18 | 2004-03-18 | Outer space carrying device for gene engineering cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1670177A CN1670177A (en) | 2005-09-21 |
| CN1316007C true CN1316007C (en) | 2007-05-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100088292A Expired - Fee Related CN1316007C (en) | 2004-03-18 | 2004-03-18 | Outer space carrying device for gene engineering cell |
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| Country | Link |
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| CN (1) | CN1316007C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103060184B (en) * | 2012-09-26 | 2015-01-14 | 中国人民解放军总医院 | Design method of space carrying device of biological samples |
| CN103060185B (en) * | 2012-09-26 | 2015-02-04 | 中国人民解放军总医院 | Spatial carrier of biological samples |
| CN103060183B (en) * | 2012-09-26 | 2015-01-21 | 中国人民解放军总医院 | Spaceship carrier of microorganisms and cells |
| CN103184150B (en) * | 2012-09-26 | 2015-02-04 | 中国人民解放军总医院 | Design method of microorganism and cell ship carrying device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2076105U (en) * | 1990-10-11 | 1991-05-01 | 中山医科大学孙逸仙纪念医院 | Disposal bacteria, fungi culture box |
| CN2147243Y (en) * | 1992-01-07 | 1993-11-24 | 黑龙江省卫生防疫站 | Cultivating bottle for testing effect of ultraviolet ray sterilizing |
| CN1382209A (en) * | 1999-07-29 | 2002-11-27 | 奥古斯丁纳斯·巴德 | Device for culturing and/or treating cells |
-
2004
- 2004-03-18 CN CNB2004100088292A patent/CN1316007C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2076105U (en) * | 1990-10-11 | 1991-05-01 | 中山医科大学孙逸仙纪念医院 | Disposal bacteria, fungi culture box |
| CN2147243Y (en) * | 1992-01-07 | 1993-11-24 | 黑龙江省卫生防疫站 | Cultivating bottle for testing effect of ultraviolet ray sterilizing |
| CN1382209A (en) * | 1999-07-29 | 2002-11-27 | 奥古斯丁纳斯·巴德 | Device for culturing and/or treating cells |
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|---|---|
| CN1670177A (en) | 2005-09-21 |
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Granted publication date: 20070516 Termination date: 20110318 |