CN110656048A - Cell culture cell and have its culture vessel - Google Patents
Cell culture cell and have its culture vessel Download PDFInfo
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- CN110656048A CN110656048A CN201911114066.2A CN201911114066A CN110656048A CN 110656048 A CN110656048 A CN 110656048A CN 201911114066 A CN201911114066 A CN 201911114066A CN 110656048 A CN110656048 A CN 110656048A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 61
- 239000012982 microporous membrane Substances 0.000 claims abstract description 42
- 239000003381 stabilizer Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 5
- 230000004308 accommodation Effects 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 4
- 238000009434 installation Methods 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 9
- 238000003756 stirring Methods 0.000 description 6
- 230000002349 favourable effect Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- -1 polytetrafluoroethylene Polymers 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000012007 large scale cell culture Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004952 Polyamide Substances 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010041 electrostatic spinning Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000002062 molecular scaffold Substances 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000008568 cell cell communication Effects 0.000 description 1
- 230000008614 cellular interaction Effects 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000006854 communication Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention relates to the field of bioengineering, and discloses a cell culture chamber and a culture vessel with the cell culture chamber, which comprise a top plate, a bottom plate, a support piece, a microporous membrane and an air bag assembly, wherein through holes are formed in the top plate and the bottom plate, the top plate and the bottom plate are arranged oppositely up and down and are connected through the support piece, the periphery of the top plate and the periphery of the bottom plate are sealed through the microporous membrane, the inner periphery of the top plate and the inner periphery of the bottom plate are sealed through the microporous membrane, so that a sealed accommodating space is defined by the top plate, the bottom plate and the microporous membrane, a sample adding hole is formed in the top plate and is communicated with the accommodating space, and the air bag assembly is arranged on the top plate and protrudes out of. The beneficial effects are that: the cell culture cell can float in the culture medium, so that cells in the cell culture cell can be fully exchanged with other outside, the cell culture cell can be prevented from directly and violently colliding with a cell culture vessel by installing the air bag, and the structural stability of the cell culture cell is kept.
Description
Technical Field
The invention relates to the field of bioengineering, in particular to a cell culture chamber and a culture vessel with the same.
Background
When in vitro large-scale cell culture, a plurality of cell culture cells are placed in a culture vessel, and the cell culture cells are accumulated at the bottom of a culture container due to the self weight, so that the gas exchange of cells in the cell culture cells is not facilitated. When stirring culture is required, the cell culture cells collide with each other or with the wall surface of the culture vessel, which not only easily damages the cell culture cells, but also generates large vibration to affect the growth of cells.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the floatable cell culture chamber with simple and reasonable structure. Another object of the present invention is to provide a culture vessel which is simple in structure, reasonable and suitable for large-scale cell culture.
The purpose of the invention is realized by the following technical scheme: the utility model provides a cell culture cell, includes roof, bottom plate, support piece, microporous membrane and gasbag subassembly, all open the through hole on roof and the bottom plate, roof and bottom plate relative setting from top to bottom to connect through support piece, connect through the microporous membrane between the periphery of roof and the periphery of bottom plate, connect through the microporous membrane between the interior week of roof and the interior week of bottom plate, so that the accommodation space who encloses between roof, bottom plate and the microporous membrane, the roof is opened there is the sampling hole, and this sampling hole and accommodation space intercommunication, the gasbag subassembly is installed in the roof to protrusion in the periphery that is located the microporous membrane in the outside.
Further, the balloon assembly comprises a balloon and a retainer ring; the gasbag is installed in the upper end of roof to protrusion in the periphery that is located the microporous membrane in the outside, the gasbag has the inflation inlet, the inflation inlet adopts the plug sealed, solid fixed ring cup joints in the periphery of gasbag, gu fixed ring is connected with the roof.
Further, the gasbag is the arc, the quantity of gasbag is at least one, the outside protrusion of gasbag is located the periphery of the microporous membrane in the outside.
Further, the gasbag is cylindrical, the quantity of gasbag is at least two, the interval sets up between the gasbag, at least one side protrusion of gasbag is located the periphery of the microporous membrane in the outside.
Further, still include the conveyer pipe, the conveyer pipe is installed in the inboard of roof, conveyer pipe and application of sample hole intercommunication, the conveyer pipe is opened has a plurality of evenly distributed's weeping hole.
Further, the conveying pipe is annular.
Further, the three-dimensional support is also included and is positioned in the accommodating space.
Further, the three-dimensional scaffold is an electrostatic spinning nanofiber scaffold.
Further, the supporting piece is columnar or arc-shaped.
Further, still include the stabilizer blade, the stabilizer blade evenly distributed is in the bottom of bottom plate.
Further, the stiffness of both the top plate and the bottom plate is greater than that of the microporous membrane.
Further, the microporous membrane is made of polyester, polycarbonate or polytetrafluoroethylene, and the aperture of the microporous membrane is 0.1-12.0 microns.
Further, the top plate and the bottom plate are made of polyethylene and polyamide.
A culture vessel comprising at least one cell culture chamber, said cell culture chamber being a cell culture chamber as described above.
Further, the cell culture chamber comprises a conduit, and the sample adding hole of the cell culture chamber is connected with the outside of the culture vessel through the conduit.
Compared with the prior art, the invention has the following advantages:
1. in the invention, through holes are arranged on the top plate and the bottom plate, and the inner and outer peripheries of the top plate and the bottom plate are sealed by the microporous membrane, so that the contact area between the small chamber and a culture medium in a culture vessel can be increased, and the material communication of cells inside and outside the small chamber is improved. By mounting the balloon assembly, the chamber can float in the culture medium, which helps to improve gas exchange between the cells in the chamber and the outside. The outside protrusion of gasbag subassembly is in the periphery that is located the microporous membrane in the outside, and the gasbag subassembly can play the effect of buffering, protection, effectively avoids cell and the wall of culture ware or other cells to take place violent striking, protects the structural integrity of cell, is favorable to the cell growth.
2. The cell culture chamber is internally provided with a plurality of three-dimensional supports, so that the inside of the chamber is more three-dimensional and is closer to the growth state in a human body; the culture area in the chamber is also increased.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view showing a cell culture chamber according to example 1 of the present invention without a balloon module attached thereto;
FIG. 2 is a schematic view showing the structure of a cell culture chamber according to example 1 of the present invention;
fig. 3 shows a top view of the top plate in embodiment 1 according to the present invention;
FIG. 4 is a schematic view showing the structure of a cell culture chamber in example 2 according to the present invention;
FIG. 5 shows a schematic construction of a culture bag according to the invention;
FIG. 6 is a schematic view showing the structure of a culture tank according to the present invention;
in the figure, 1 is a top plate; 2 is a bottom plate; 3 is a microporous membrane; 4 is a through hole; 5 is a sample adding hole; 6 is an air bag; 7 is a fixed ring; 8 is an inflation inlet; 9 is a conveying pipe; 10 is a liquid leakage hole; 11 is a support leg; 12 is a culture bag; 13 is a conduit; 14 is a culture tank; 15 is a stirring paddle; 16 is a cell culture chamber.
Detailed Description
The invention is further illustrated by the following figures and examples.
Example 1:
the cell culture chamber is suitable for adherent culture or suspension culture; including but not limited to monolayer static culture using petri dishes, dynamic suspension culture using culture bags, or culture using stirred culture tanks, etc.
The cell culture chamber fully considers the influence of intercellular interaction on cells cultured in vitro in the large-scale cell culture process, and the co-cultured cells indirectly have positive influence on the growth state of the applied cells through a membrane technology means and a co-culture mode. Of course, the technique is also applicable to the study of various cell interactions (indirect contact).
The cell culture chamber 16 shown in fig. 1 and 2 comprises a top plate 1, a bottom plate 2, a support, a microporous membrane 3 and an air bag assembly, wherein through holes 4 are formed in the top plate 1 and the bottom plate 2, the top plate 1 and the bottom plate 2 are arranged oppositely up and down and are connected through the support, the outer periphery of the top plate 1 is connected with the outer periphery of the bottom plate 2 through the microporous membrane 3, the inner periphery of the top plate 1 is connected with the inner periphery of the bottom plate 2 through the microporous membrane 3, so that a containing space enclosed among the top plate 1, the bottom plate 2 and the microporous membrane 3 is formed, the top plate 1 is provided with a sample adding hole 5, the sample adding hole 5 is communicated with the containing space, and the air bag assembly is installed on the top plate 1 and protrudes out of the outer periphery of the.
The top plate 1 and the bottom plate 2 may be circular, rectangular or other shapes. Through holes 4 are arranged on the top plate 1 and the bottom plate 2, and the inner and outer peripheries of the top plate 1 and the bottom plate 2 are connected by adopting microporous films 3. The arrangement can increase the contact area between the microporous membrane 3 and the culture medium in the culture vessel and improve the material communication between the cells inside and outside the chamber. The top plate 1 is provided with an air bag assembly, and the outer side of the air bag assembly protrudes out of the periphery of the microporous membrane 3 positioned at the outer side. The cell culture chamber floats in the cell culture vessel, gas exchange between cells in the chamber and the outside is facilitated to be improved, severe impact between the chamber and the wall surface of the culture vessel or other chambers is avoided, the structural integrity of the chamber is protected, and cell growth is facilitated.
The air bag assembly comprises an air bag 6 and a fixing ring 7; the gasbag 6 is installed in the upper end of roof 1 to protrusion in the periphery that is located the microporous membrane 3 in the outside, gasbag 6 has inflation inlet 8, inflation inlet 8 adopts the plug sealed, solid fixed ring 7 cup joints in the periphery of gasbag 6, gu fixed ring 7 is connected with roof 1. Be provided with the solid fixed ring 7 that a plurality of silica gel or rubber were made on the gasbag 6, be used for fixed gasbag 6 on the one hand, have the indicative function on the one hand, in case gaseous reduction in the gasbag 6, produce the space between gasbag 6 and the solid fixed ring 7, remind the operator gasbag 6 to need inject into gas. The fixing ring is connected with the top plate by ultrasonic waves or adhesive. In addition to being fixed to the top plate 1 by the fixing ring 7, the airbag 6 is also connected to the top plate 1 by an adhesive. When the gas in the air bag 6 is insufficient, the gas is injected into the air bag 6 through the inflating opening 8.
As shown in fig. 2, the air bags 6 are arc-shaped, and when the number of the air bags 6 is one, the outer sides of the air bags 6 all protrude out of the periphery of the microporous membrane 3 positioned at the outer side; when the number of the air bags 6 is two, the two air bags 6 can be oppositely arranged on the top plate 1, and the outer sides of the air bags 6 protrude out of the periphery of the microporous membrane 3. The bladder 6 may be of other shapes, for particular uses.
As shown in fig. 3, the sample adding device further comprises a delivery pipe 9, wherein the delivery pipe 9 is installed on the inner side of the top plate 1, the delivery pipe 9 is communicated with the sample adding hole 5, and the delivery pipe 9 is provided with a plurality of uniformly distributed liquid leaking holes 10. The liquid leaking holes 10 may have the same diameter, or may have a gradually increasing diameter as they are farther from the wells.
Wherein the delivery pipe 9 is annular. The cells can be uniformly distributed in the small chamber by arranging the conveying pipe 9, and the cells in the small chamber can be uniformly distributed and grow by matching with manual shaking.
The three-dimensional support is positioned in the accommodating space. The three-dimensional scaffold is an electrostatic spinning nanofiber scaffold. The arrangement makes the interior of the small chamber more three-dimensional and closer to the growth state in the human body; the culture area in the chamber is also increased. When in specific use, the three-dimensional bracket can also be made by other methods.
The supporting piece is columnar or arc-shaped. The supporting members are spaced between the top plate 1 and the bottom plate 2, and support the top plate 1 and the bottom plate 2 to form a three-dimensional space.
Still include stabilizer blade 11, stabilizer blade 11 evenly distributed is in the bottom of bottom plate 2. The cell crushing device is used for preventing cells at the bottom of the culture vessel from being crushed when the cell contacts the bottom of the culture vessel where the cell is located.
The stiffness of the top plate 1 and the bottom plate 2 is greater than that of the microporous membrane 3. This be provided with do benefit to and improve the inside and outside material exchange of cell, can also guarantee the structural stability of cell.
The microporous membrane 3 is made of polyester, polycarbonate, polytetrafluoroethylene or other materials, and the aperture of the microporous membrane is 0.1-12.0 mu m. The top plate 1 and the bottom plate 2 are made of polyethylene, polyamide and other materials. The microporous membrane 3 is connected with the top plate 1 and the bottom plate 2 by ultrasonic waves or adhesive.
The cell culture chamber 16 is most suitable for specially-made culture vessels (such as large and deep culture vessels), and during operation, a certain culture medium is injected into the culture vessel, cells are injected into the chamber, and the culture medium in the culture vessel and the cells in the cell culture chamber 16 are shaken up. Finally, adding the target cells into a culture vessel, and shaking up for culture.
A culture vessel comprising at least one cell culture chamber 16, said cell culture chamber 16 being a cell culture chamber 16 as described above. The culture vessel is not limited to a culture flask, a culture bag 12, a culture tank 14, and the like.
The cell culture chamber 16 further comprises a conduit 13, and the sample adding hole 5 of the cell culture chamber 16 is connected with the outside of the culture vessel through the conduit 13. As shown in FIG. 5, the cell culture chamber 16 floats in the culture bag 12, the cell culture chamber 16 is connected to the outside of the culture bag 12 through the conduit 13, and nutrients or other substances required for the test can be injected into the cell culture chamber 16 through the conduit 13.
As shown in FIG. 6, the cell culture chamber 16 floats in the culture tank 14 with the paddle, and the cell culture chamber 16 is rotated in the culture tank 14 by the paddle, whereby the uniformity of the substances in the cell culture chamber can be improved, and the exchange of the substances and gases can be promoted. When the stirring paddle 15 is used, the rod part of the stirring paddle 15 can also penetrate through the through hole of the cell culture chamber 16, so that the cell culture chamber 16 is sleeved on the stirring paddle 15, and the cell culture chamber rotates along with the stirring paddle 15.
Example 2:
the present example is the same as example 1 except for the following technical features:
as shown in fig. 4, the airbags 6 are cylindrical, the number of the airbags 6 is two, the airbags 6 are arranged at intervals, the two airbags 6 are respectively positioned at two sides of the top plate 1, and three sides of each airbag 6 protrude out of the periphery of the microporous membrane positioned at the outer side. The buoyancy of this setting multiplicable cell makes the cell float on the culture system, is favorable to increasing the gas exchange of cell in the cell, prevents effectively that cell and cell culture household utensils from taking place violent collision, protects the structural stability of cell, is favorable to the cell to grow.
Example 3:
the present example is the same as example 1 except for the following technical features:
the gasbag 6 is cylindrical, the quantity of gasbag 6 is three, gasbag 6 is cyclic annular distribution, installs on roof 1, each one side protrusion of gasbag 6 is in the periphery that is located the microporous membrane 3 in the outside. The buoyancy of this setting multiplicable cell makes the cell float on the culture system, is favorable to increasing the gas exchange of cell in the cell, prevents effectively that cell and cell culture household utensils from taking place violent collision, protects the structural stability of cell, is favorable to the cell to grow.
The above-mentioned embodiments are preferred embodiments of the present invention, and the present invention is not limited thereto, and any other modifications or equivalent substitutions that do not depart from the technical spirit of the present invention are included in the scope of the present invention.
Claims (10)
1. A cell culture chamber, comprising: including roof, bottom plate, support piece, microporous membrane and gasbag subassembly, all open the through hole on roof and the bottom plate, roof and bottom plate relative setting from top to bottom to connect through support piece, connect through the microporous membrane between the periphery of roof and the periphery of bottom plate, connect through the microporous membrane between the interior week of roof and the interior week of bottom plate, so that the accommodation space who encloses between roof, bottom plate and the microporous membrane, the roof is opened there is the sampling hole, and this sampling hole and accommodation space intercommunication, gasbag subassembly installation is in the roof to the protrusion is in the periphery that is located the microporous membrane in the outside.
2. A cell culture chamber according to claim 1, wherein: the air bag assembly comprises an air bag and a fixing ring; the gasbag is installed in the upper end of roof to protrusion in the periphery that is located the microporous membrane in the outside, the gasbag has the inflation inlet, the inflation inlet adopts the plug sealed, solid fixed ring cup joints in the periphery of gasbag, gu fixed ring is connected with the roof.
3. A cell culture chamber according to claim 2, wherein: the gasbag is the arc, the quantity of gasbag is at least one, the outside protrusion of gasbag is in the periphery that is located the microporous membrane in the outside.
4. A cell culture chamber according to claim 2, wherein: the gasbag is cylindrical, the quantity of gasbag is at least two, the interval sets up between the gasbag, at least one side protrusion of gasbag is in the periphery that is located the microporous membrane in the outside.
5. A cell culture chamber according to claim 1, wherein: still include the conveyer pipe, the conveyer pipe is installed in the inboard of roof, conveyer pipe and application of sample hole intercommunication, the conveyer pipe is opened has a plurality of evenly distributed's weeping hole.
6. A cell culture chamber according to claim 1, wherein: the three-dimensional support is positioned in the accommodating space.
7. A cell culture chamber according to claim 1, wherein: still include the stabilizer blade, stabilizer blade evenly distributed is in the bottom of bottom plate.
8. A cell culture chamber according to claim 1, wherein: the stiffness of the top plate and the bottom plate is greater than that of the microporous membrane.
9. A culture vessel, characterized in that: comprising at least one cell culture chamber according to any one of claims 1 to 8.
10. A culture vessel according to claim 9, wherein: the cell culture chamber also comprises a conduit, and the sample adding hole of the cell culture chamber is connected with the outside of the culture vessel through the conduit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911114066.2A CN110656048A (en) | 2019-11-14 | 2019-11-14 | Cell culture cell and have its culture vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911114066.2A CN110656048A (en) | 2019-11-14 | 2019-11-14 | Cell culture cell and have its culture vessel |
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| CN110656048A true CN110656048A (en) | 2020-01-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115287261A (en) * | 2022-08-15 | 2022-11-04 | 中国人民解放军空军军医大学 | Primary neural stem cell in-vitro three-dimensional culture system and culture method thereof |
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|---|---|---|---|---|
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| US20030143727A1 (en) * | 2002-01-31 | 2003-07-31 | King-Ming Chang | Cell-cultivating device |
| CN204737966U (en) * | 2015-05-28 | 2015-11-04 | 深圳职业技术学院 | Multiple cell culture apparatus |
| CN205347445U (en) * | 2016-01-22 | 2016-06-29 | 天津卫凯生物工程有限公司 | Cell culture fibre membrane support frame, cell culture support and cell culture device |
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| CN207362256U (en) * | 2017-10-18 | 2018-05-15 | 上海柏根生物科技有限公司 | A kind of suspension cell culture plate |
| CN207854797U (en) * | 2018-01-24 | 2018-09-14 | 于茗远 | Inflation rises floating type holothruian cultures device |
| CN209024557U (en) * | 2018-08-03 | 2019-06-25 | 中享生命科技有限公司 | A kind of floated tissue culture plate |
| CN211471436U (en) * | 2019-11-14 | 2020-09-11 | 广州瑞铂茵健康管理咨询有限公司 | Cell culture cell and have its culture vessel |
-
2019
- 2019-11-14 CN CN201911114066.2A patent/CN110656048A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990000595A1 (en) * | 1988-07-05 | 1990-01-25 | Banes Albert J | Floating cell culture device and method |
| US5122470A (en) * | 1988-07-05 | 1992-06-16 | Banes Albert J | Floating cell culture device and method |
| US20030143727A1 (en) * | 2002-01-31 | 2003-07-31 | King-Ming Chang | Cell-cultivating device |
| CN204737966U (en) * | 2015-05-28 | 2015-11-04 | 深圳职业技术学院 | Multiple cell culture apparatus |
| CN205347445U (en) * | 2016-01-22 | 2016-06-29 | 天津卫凯生物工程有限公司 | Cell culture fibre membrane support frame, cell culture support and cell culture device |
| CN206843507U (en) * | 2017-06-08 | 2018-01-05 | 北京焕生汇生物技术研究院 | A kind of suspension cell culture device |
| CN207362256U (en) * | 2017-10-18 | 2018-05-15 | 上海柏根生物科技有限公司 | A kind of suspension cell culture plate |
| CN207854797U (en) * | 2018-01-24 | 2018-09-14 | 于茗远 | Inflation rises floating type holothruian cultures device |
| CN209024557U (en) * | 2018-08-03 | 2019-06-25 | 中享生命科技有限公司 | A kind of floated tissue culture plate |
| CN211471436U (en) * | 2019-11-14 | 2020-09-11 | 广州瑞铂茵健康管理咨询有限公司 | Cell culture cell and have its culture vessel |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115287261A (en) * | 2022-08-15 | 2022-11-04 | 中国人民解放军空军军医大学 | Primary neural stem cell in-vitro three-dimensional culture system and culture method thereof |
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