CN207362256U - A kind of suspension cell culture plate - Google Patents
A kind of suspension cell culture plate Download PDFInfo
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- CN207362256U CN207362256U CN201721338405.1U CN201721338405U CN207362256U CN 207362256 U CN207362256 U CN 207362256U CN 201721338405 U CN201721338405 U CN 201721338405U CN 207362256 U CN207362256 U CN 207362256U
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- culture
- culture plate
- plate
- permeable membrane
- colour developing
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Abstract
The utility model discloses a kind of suspension cell culture plate, including hydrophobic permeable membrane, develop the color ring and culture plate, culture plate surface is uniformly distributed some culture holes, culture hole is surrounded with colour developing ring for cylinder and surrounding, label is provided with below colour developing ring, culture plate is fixed on backplate surface, bottom plate both sides are provided with frosted area, culture hole bottom is fixed with cylinder seal groove, it is connected filled with sterile water inside cylinder seal groove and by pipeline with colour developing ring, and cylinder sealing trench bottom is fixed with hydrophobic permeable membrane, by creating newly-installed hydrophobic permeable membrane, increase the area of gas exchanges, solve the problems, such as that gas permeability is insufficient；Whether by creating newly-installed colour developing ring, the effect of cell and culture medium can added in the culture hole at the first time by having reached, solve the problems, such as repetition leakage plus；By creating newly-installed bottom plate, the effect for being capable of in the vertical direction placement multilayer culture plate is reached, has greatlyd save using space, solve the problems, such as to plant oneself.
Description
Technical field
A kind of culture plate is the utility model is related to, is specially a kind of suspension cell culture plate.
Background technology
Cell growth does not depend on support surface, is grown in nutrient solution in suspended state, and this kind of cell is known as suspending thin
Born of the same parents, the culture that suspends refer to a kind of in the fluid nutrient medium for constantly being stirred or being shaken, the unicellular and small cell cluster of culture
Tissue culture system, be non-anchorage-dependent cell a kind of training method.
Existing suspension cell culture plate in use, there are many problems, such as：There are asking for gas permeability deficiency
Topic, existing Tissue Culture Plate are all made of culture plate and culture hole, since atmospheric density is less than carbon dioxide, cause to train
It is very big to support gas concentration lwevel above hole, it is not high with air change efficiency, and then the saturation of suspension cell is influenced, limit thin
The quantity of born of the same parents' culture so that experimenter has to use many culture holes at the same time, and wasting manpower and material resources, also increase pollution
Risk；Leaked the problem of adding in the presence of repetition, in biomedicine experiment, experimenter need to be often loaded into cell culture well, due to anti-
Be added with sample, easily occur when absent minded leakage plus or repeat to be loaded, so as to influence experimental result；It is big in the presence of planting oneself
Problem, culture plate occupy many positions in the horizontal direction, and vertical space availability ratio is not high, and sometimes experiment needs many types
Cell mass propgation, cause the tiling of many culture plates together, occupy substantial amounts of space.
In conclusion existing culture plate there are gas permeability deficiency, repeat leakage adduction and plant oneself big etc. three urgently to solve greatly
Certainly the problem of.
The content of the invention
The technical problems to be solved in the utility model is to overcome existing culture plate during the work time, and gas permeability is insufficient, again
Leakage adduction plants oneself the defects of big again, there is provided a kind of suspension cell culture plate, so as to solve the above problems.
In order to solve the above-mentioned technical problem, the utility model provides following technical solution：
A kind of suspension cell culture plate of the utility model, including hydrophobic permeable membrane, colour developing ring and culture plate, cultivate plate surface
Some culture holes are uniformly distributed, culture hole is surrounded with colour developing ring for cylinder and surrounding, is provided with label below colour developing ring, cultivates
Plate is fixed on backplate surface, and bottom plate both sides are provided with frosted area, and culture hole bottom is fixed with cylinder seal groove, in cylinder seal groove
Portion is connected filled with sterile water and by pipeline with colour developing ring, and cylinder sealing trench bottom is fixed with hydrophobic permeable membrane.
As a kind of optimal technical scheme of the utility model, bottom plate and culture plate are using high-quality, transparent non-renewable poly-
Ethene, which is integrally formed, to be made, and culture plate is rectangular, and rectangular aspect is less than bottom plate, conveniently withholds, and has high sterile level, right and wrong
The ideal chose of attached cell suspension culture.
As a kind of optimal technical scheme of the utility model, hydrophobic permeable membrane is polytetrafluoroethylene film or Kynoar
Film, and hydrophobic permeable membrane is circle, corresponds to indentation, there in bottom plate bottom surface upper and lower surface by inlaying to fill up, increases gas exchanges
Area, add the gas permeability of culture hole, inlay and be arranged on indentation, there and can also reduce the cost and weight of device.
As a kind of optimal technical scheme of the utility model, colour developing ring inner homogeneous be filled with copper sulphate, colour developing ring with
Tube contacts place is provided with filter screen, and hydrophobic permeable membrane is provided with the top of the ring that develops the color, and copper sulphate meets water colour developing, effectively prompting
The addition situation of experimenter's culture hole, avoid repetition leakage plus, filter screen prevents copper sulphate to fall, and hydrophobic permeable membrane is protected
The water inside display device has been demonstrate,proved to be not vaporized.
As a kind of optimal technical scheme of the utility model, gasket is bonded with plate inner wall, gasket uses bullet
Property rubber be made, can superposition when in space formed sealing, avoid polluting.
The beneficial effect that the utility model is reached is：The utility model is increased by creating newly-installed hydrophobic permeable membrane
The area of gas exchanges, adds the gas permeability of culture hole, increases the cell in hole and accommodates and reach the standard grade, solves gas permeability not
The problem of sufficient；Whether by creating newly-installed colour developing ring, having reached can be at the first time in culture hole addition cell and training
The effect of foster base, can effectively avoid repeating to leak adding, and simple in structure, of low cost, solve the problems, such as that repetition leakage adds；It is logical
Cross and create newly-installed bottom plate, reached the effect for being capable of in the vertical direction placement multilayer culture plate, greatlyd save using empty
Between, conventional efficient is improved, solves the problems, such as to plant oneself.
Brief description of the drawings
Attached drawing is used to provide a further understanding of the present invention, and a part for constitution instruction, with this practicality
New embodiment is used to explain the utility model together, does not form the limitation to the utility model.
In the accompanying drawings：
Fig. 1 is the utility model overall structure diagram；
Fig. 2 is the utility model culture hole partial structural diagram；
Fig. 3 is the utility model bottom schematic view；
Figure label：1st, bottom plate；2nd, culture plate；3rd, frosted area；4th, label；5th, culture hole；6th, develop the color ring；7th, pipeline；8、
Hydrophobic permeable membrane.
Embodiment
The preferred embodiment of the utility model is illustrated below in conjunction with attached drawing, it will be appreciated that described herein excellent
Select embodiment to be only used for describing and explaining the present invention, be not used to limit the utility model.
, it is necessary to explanation in the description of the utility model, the instruction such as term " vertical ", " on ", " under ", " level "
Orientation or position relationship are based on orientation shown in the drawings or position relationship, are for only for ease of description the utility model and letter
Change description, rather than instruction or imply signified device or element must have specific orientation, with specific azimuth configuration and
Operation, therefore it is not intended that limitation to the utility model.
In the description of the utility model, it is also necessary to which explanation, unless otherwise clearly defined and limited, term " are set
Put ", " installation ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected,
Or it is integrally connected；Can mechanically connect or be electrically connected；It can be directly connected, intermediary can also be passed through
It is indirectly connected, can is the connection inside two elements.For the ordinary skill in the art, can be according to specific
Situation understands concrete meaning of the above-mentioned term in the utility model.
Embodiment：As shown in Figs. 1-3, a kind of suspension cell culture plate of the utility model offer, including hydrophobic permeable membrane 8,
Develop the color ring 6 and culture plate 2, and 2 surface of culture plate is uniformly distributed some culture holes 5, and culture hole 5 is cylinder and is around surrounded with aobvious
Colour circle 6,6 lower section of colour developing ring are provided with label 4, and culture plate 2 is fixed on 1 surface of bottom plate, and 1 both sides of bottom plate are provided with frosted area 3, training
Support 5 bottom of hole and be fixed with cylinder seal groove, be connected inside cylinder seal groove filled with sterile water and by pipeline 7 with colour developing ring 6,
And cylinder sealing trench bottom is fixed with hydrophobic permeable membrane 8.
Specifically, a kind of suspension cell culture plate of the utility model, bottom plate 1 and culture plate 2 using it is high-quality, transparent it is non-again
Raw polyethylene, which is integrally formed, to be made, and culture plate 2 is rectangular, and rectangular aspect is less than bottom plate 1, conveniently withholds, and has high sterile water
It is flat, it is that non-adherent cell suspends the ideal chose of culture, hydrophobic permeable membrane 8 is polytetrafluoroethylene film or polyvinylidene fluoride film, and
Hydrophobic permeable membrane 8 is circle, corresponds to indentation, there in 1 bottom surface upper and lower surface of bottom plate by inlaying to fill up, increases gas exchanges
Area, adds the gas permeability of culture hole 5, inlays and is arranged on indentation, there and can also reduce the cost and weight of device, develop the color ring 6
Inner homogeneous is filled with copper sulphate, and colour developing ring 6 and pipeline 7 contact place and be provided with filter screen, and is provided with the top of the ring 6 that develops the color thin
Water ventilated membrane 8, copper sulphate meet water colour developing, have effectively prompted the addition situation of experimenter's culture hole 5, avoid repetition leakage and add,
Filter screen prevents copper sulphate to fall, and hydrophobic permeable membrane 8 ensure that the water inside display device is not vaporized, on 1 inner wall of bottom plate
Gasket is bonded with, gasket is made of elastic rubber, sealing can be formed in space when superposition, be avoided dirt
Dye.
Operation principle：Laboratory, can one hand hold culture plate 2, hold the stability in frosted area 3, adding device,
When adding cell and culture medium, cell culture medium falls in culture hole 5, adds the quality of culture hole 5, the culture hole aggravated
Sterile water inside the cylinder seal groove of bottom is squeezed into pipeline 7 by 5, and sterile water is entered in colour developing ring 6 through piping 7, sterile
Water contact colour developing with the copper sulphate for the ring 6 that develops the color, and represents the culture hole 5 plus punishment in advance, avoids repeating to leak adding, and experimenter is by cell
Multiple culture plates 2 on the labels 4, easy to distinguish, all after the completion of addition, can be stacked together by the information records such as title, bottom
Plate 1 is covered on culture plate 2.
Finally it should be noted that：It these are only the preferred embodiment of the utility model, be not limited to this practicality
It is new, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art,
It can still modify the technical solution described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement.Where within the spirit and principles of the present invention, any modification, equivalent replacement, improvement and so on, should all include
Within the scope of protection of the utility model.
Claims (5)
1. a kind of suspension cell culture plate, it is characterised in that including hydrophobic permeable membrane（8）, colour developing ring（6）And culture plate（2）, training
Support plate（2）Surface is uniformly distributed some culture holes（5）, culture hole（5）To be surrounded with colour developing ring around cylinder and top（6）,
Develop the color ring（6）Lower section is provided with label（4）, culture plate（2）It is fixed on bottom plate（1）Surface, bottom plate（1）Both sides are provided with frosted area
（3）, culture hole（5）Bottom is fixed with cylinder seal groove, is filled with sterile water inside cylinder seal groove and passes through pipeline（7）With showing
Colour circle（6）Connection, and cylinder sealing trench bottom is fixed with hydrophobic permeable membrane（8）.
A kind of 2. suspension cell culture plate according to claim 1, it is characterised in that bottom plate（1）And culture plate（2）Using
High-quality, transparent non-renewable polyethylene, which is integrally formed, to be made, and culture plate（2）Rectangular, rectangular aspect is less than bottom plate（1）.
A kind of 3. suspension cell culture plate according to claim 1, it is characterised in that hydrophobic permeable membrane（8）For polytetrafluoro
Vinyl film or polyvinylidene fluoride film, and hydrophobic permeable membrane（8）For circle, filled up by inlaying in bottom plate（1）Bottom surface upper and lower surface
Corresponding indentation, there.
A kind of 4. suspension cell culture plate according to claim 1, it is characterised in that colour developing ring（6）Inner homogeneous is filled
There is copper sulphate, develop the color ring（6）And pipeline（7）Contact place is provided with filter screen, and the ring that develops the color（6）Top is provided with hydrophobic, air-permeability
Film（8）.
A kind of 5. suspension cell culture plate according to claim 1, it is characterised in that bottom plate（1）It is bonded with inner wall close
Packing, gasket are made of elastic rubber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721338405.1U CN207362256U (en) | 2017-10-18 | 2017-10-18 | A kind of suspension cell culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721338405.1U CN207362256U (en) | 2017-10-18 | 2017-10-18 | A kind of suspension cell culture plate |
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| Publication Number | Publication Date |
|---|---|
| CN207362256U true CN207362256U (en) | 2018-05-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201721338405.1U Active CN207362256U (en) | 2017-10-18 | 2017-10-18 | A kind of suspension cell culture plate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109738347A (en) * | 2019-02-25 | 2019-05-10 | 广州牛顿光学研究院有限公司 | A kind of cell count Special sample pond and production method |
| CN110656048A (en) * | 2019-11-14 | 2020-01-07 | 广州瑞铂茵健康管理咨询有限公司 | Cell culture cell and have its culture vessel |
-
2017
- 2017-10-18 CN CN201721338405.1U patent/CN207362256U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109738347A (en) * | 2019-02-25 | 2019-05-10 | 广州牛顿光学研究院有限公司 | A kind of cell count Special sample pond and production method |
| CN110656048A (en) * | 2019-11-14 | 2020-01-07 | 广州瑞铂茵健康管理咨询有限公司 | Cell culture cell and have its culture vessel |
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